Detection device for simulating soil respiration rate

By designing an indoor simulation experimental device and using a detection device that simulates soil respiration rate, the problem of difficulty in accurately measuring soil respiration dynamics in the prior art is solved, accurate measurement under indoor conditions is achieved, large-scale experimental applications are supported, and new tools are provided to study soil carbon cycle and global climate change.

CN222979543UActive Publication Date: 2025-06-13GUIZHOU MINZU UNIV
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Patent Information

Application Number
CN202421718142.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-19
Publication Date
2025-06-13
Estimated Expiration
2034-07-19

AI Technical Summary

Technical Problem

It is difficult for the prior art to accurately measure soil respiration dynamics under different land use modes and in different soil levels, and there are errors and inconveniences in field experiments.

Method used

An indoor simulation experimental device is designed, including a box, a lid, a syringe and a gas collection tube. By injecting culture liquid into the soil sample and collecting exhaust gas, the soil respiration rate is calculated using a gas detection component. The device allows for independent cultivation of multiple batches, supporting accurate determination under different land use conditions.

Benefits of technology

It realizes the accurate determination of soil respiration rate under indoor conditions, reduces the error and inconvenience of field experiments, supports large-scale experimental applications, and provides new tools for studying soil carbon cycle and global climate change.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a detection device for simulating the respiration rate of soil, which comprises a box body (1) provided with a containing cavity (11) with an open end, and the containing cavity (11) is internally provided with a soil sample (200); the cover body (2) detachably covers the open end of the box body (1) so as to form a closed accommodating cavity (11); wherein two mounting holes (21) communicated with the accommodating cavity (11) are formed in the cover body (2); the injector (5) is communicated with the accommodating cavity (11) through one of the mounting holes (21) and is configured to be used for injecting culture liquid into the accommodating cavity (11); one end of the gas collecting pipe (6) is communicated with the other mounting hole (21), and the other end of the gas collecting pipe (6) is communicated with a gas detection assembly.
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Description

Technical Field

[0001] The utility model relates to the technical field of measuring soil respiration rate, in particular to a detection device for indoor simulation of measuring soil respiration rate under different soils and different land use patterns. Background Technique

[0002] The soil respiration process, as a key link in the global terrestrial ecosystem carbon cycle, plays a crucial role in maintaining ecological balance and global carbon balance. The mineralization and transformation process of soil organic carbon, that is, soil respiration, is not only the second largest carbon flow pathway within the terrestrial ecosystem, but also its contribution to global carbon emissions accounts for a significant proportion, approximately 60% to 90%. In this process, the subtle fluctuations of soil respiration are extremely sensitive to environmental changes, so it has important indicator significance for predicting and evaluating the impact of climate change. In the carbon exchange between soil and atmosphere, microorganisms play an extremely crucial role. Through their metabolic activities, they convert the organic matter in the soil into carbon dioxide and directly participate in the mineralization process of soil organic carbon. Therefore, in-depth study of soil respiration rate and its influencing factors can not only deepen our understanding of the carbon cycle mechanism of the terrestrial ecosystem, but also provide a new perspective for exploring the role of soil microorganisms in the global carbon cycle.

[0003] Currently, the measurement of soil respiration rate is mainly based on field experiments, which can be roughly divided into two types. One is to use a portable soil respiration system instrument for measurement. This instrument is mainly used to measure the instantaneous soil respiration rate and can continuously detect soil respiration changes for 24 hours. However, this instrument is relatively expensive, cannot be used on a large scale, and is greatly affected by regional factors and is not suitable for forests with dense vegetation. Another method is to set up a closed area in the field, similar to a closed box, and regularly collect and measure the amount of gases such as CO 2 and CH 4 emitted. This method is also divided into two categories: determination by alkali solution absorption and acid-base neutralization titration and determination by instruments for the concentration of CO 2 and CH 4 gases. However, this method is mostly applicable to single plots and is not measured under laboratory conditions, which is prone to errors.

[0004] In view of this, in order to more accurately explore the soil respiration dynamics under different land use patterns and different soil layers, there is an urgent need to develop a new type of indoor simulation experimental device. Summary of the Invention

[0005] In response to the problems and needs mentioned above, this solution proposes a detection device for simulating soil respiration rate. Due to the following technical features, it can achieve the above technical objectives and bring many other technical effects.

[0006] An object of the present utility model is to provide a detection device for simulating soil respiration rate, comprising:

[0007] A box body, which forms a containing cavity with an open end, wherein a soil sample is placed in the containing cavity;

[0008] A cover body, detachably covering the open end of the box body to form a sealed containing cavity; wherein, two mounting holes communicating with the containing cavity are formed on the cover body;

[0009] A syringe, which is communicated with the containing cavity through one of the mounting holes and is configured to inject a culture liquid into the containing cavity;

[0010] A gas collection tube, one end of which is communicated with the other mounting hole, and the other end of which is communicated with a gas detection component.

[0011] In this technical solution, conduct research on the sampling site of the soil to be measured, master environmental characteristics such as precipitation and temperature, measure the volume of the box body, count the volume of the soil to be cultured, the volume of the alkali solution, etc. (ignoring the volume occupied by the materials of the containers for containing the alkali solution and the soil), and calculate the volume of the air in the sealed box during cultivation; for the soil samples transported back by refrigerated transportation, put them into the box body according to different requirements (small amount: 0 - 100 g, medium amount: 10 - 500 g, large amount: 500 - 1000 g, and extra-large amount: more than 1000 g), adjust the temperature of the incubator or the indoor temperature to the appropriate temperature of 25 °C and culture for 3 d. During this period, water or nutrient solution can be added according to requirements. Wait until the soil is in the state at the time of sampling before starting sealed cultivation; during sealed cultivation, use a syringe to inject liquid into the containing cavity, and also keep the water full at all times during cultivation to avoid air entry and reduce the systematic error of the data; collect the discharged gas in the containing cavity through the gas collection tube, and calculate the soil respiration rate by the gas detection component; this detection device allows for multi-batch and independent cultivation of soil samples, and supports accurate determination of soil respiration rate under different land use conditions. The materials of this device are easy to obtain, the cost is low, and it is convenient for large-scale experimental applications. It can not only freely adjust the temperature and humidity in the experiment to better simulate natural conditions, but also effectively avoid the interference of meteorological and regional influencing factors, providing a new experimental tool for soil carbon cycle and global climate change research. In addition, the usage method of this device is simple, providing an effective technical means for studying the effects of different land use patterns and soil layers on soil respiration rate.

[0012] In addition, the detection device for simulating soil respiration rate according to the present utility model may further have the following technical features:

[0013] In an example of the present utility model, it further comprises: at least one valve,

[0014] It is installed on at least one of the two mounting holes and is configured to control the injection amount of the culture liquid in the syringe and / or the discharge amount of the gas in the accommodation cavity.

[0015] In an example of the present utility model, a rubber tube is also connected between the syringe and the valve and between the gas collection tube and the valve.

[0016] In an example of the present utility model, the culture liquid includes tap water, deionized water or compound nutrient solution.

[0017] In an example of the present utility model, the box body includes a transparent plastic part, a translucent plastic part or an opaque plastic part.

[0018] In an example of the present utility model, the gas detection component includes: a gas chromatograph or an infrared carbon dioxide detector.

[0019] In an example of the present utility model, it further includes: a gas collection bag,

[0020] which is communicated with the gas collection tube, is used to collect the gas discharged from the mounting hole, and place it in the gas detection component for detection.

[0021] Another object of the present utility model is to propose a detection device for simulating the soil respiration rate, including:

[0022] A box body, forming an accommodation cavity with an open end, wherein a soil sample is placed in the accommodation cavity;

[0023] A cover body, detachably covering the open end of the box body to form a sealed accommodation cavity; wherein, an installation hole communicated with the accommodation cavity is opened on the cover body;

[0024] A syringe, which is communicated with the accommodation cavity through the installation hole and is configured to inject a culture liquid into the accommodation cavity;

[0025] A beaker, containing an alkaline solution inside, and is arranged in the accommodation cavity and above the soil sample.

[0026] In an example of the present utility model, it further includes: a valve,

[0027] which is installed in the installation hole and is configured to control the injection amount of the culture liquid in the syringe.

[0028] In an example of the present utility model, the culture liquid includes tap water, deionized water or compound nutrient solution.

[0029] In the following, the optimal embodiments of implementing the present utility model will be described in more detail with reference to the accompanying drawings, so as to facilitate the understanding of the features and advantages of the present utility model. Brief Description of the Drawings

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the accompanying drawings of the embodiments of the present utility model will be briefly introduced below. Among them, the accompanying drawings are only used to show some embodiments of the present utility model, rather than limiting all embodiments of the present utility model thereto.

[0031] Figure 1 A detection device for simulating soil respiration rate according to one embodiment of the present utility model;

[0032] Figure 2 A detection device for simulating soil respiration rate according to another embodiment of the present utility model;

[0033] Figure 3 A detection device for simulating soil respiration rate according to still another embodiment of the present utility model.

[0034] List of Reference Numerals:

[0035] Soil sample 200;

[0036] Detection device 100;

[0037] Box body 1;

[0038] Accommodation cavity 11;

[0039] Cover body 2;

[0040] Mounting hole 21;

[0041] Valve 3;

[0042] Hose 4;

[0043] Syringe 5;

[0044] Gas collection tube 6;

[0045] Gas collection bag 7;

[0046] Beaker 8;

[0047] Backing plate 9;

[0048] Infrared carbon dioxide detector 10. Detailed Description of the Preferred Embodiment

[0049] In order to make the objectives, technical solutions, and advantages of the technical solution of the present utility model clearer, the technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the drawings of the specific embodiments of the present utility model. The same reference numerals in the drawings represent the same components. It should be noted that the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the described embodiments of the present utility model without creative efforts fall within the scope of protection of the present utility model.

[0050] Unless otherwise defined, the technical terms or scientific terms used herein shall have the ordinary meanings understood by those of ordinary skill in the art to which the present utility model pertains. The terms "first", "second", and similar terms used in the description and claims of the patent application of the present utility model do not indicate any order, quantity, or importance, but are only used to distinguish different components. Similarly, terms such as "a" or "an" do not necessarily indicate a quantity limitation. Terms such as "comprising" or "including" mean that the elements or items appearing before the term cover the elements or items listed after the term and their equivalents, without excluding other elements or items. Terms such as "connected" or "coupled" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. Terms such as "upper", "lower", "left", "right", etc. are only used to represent relative positional relationships, and when the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0051] According to a detection device 100 for simulating soil respiration rate according to the first aspect of the present utility model, as Figure 1 、 Figure 2 shown, it includes:

[0052] A box body 1, which forms a receiving cavity 11 with an open end. Among them, a soil sample 200 is placed in the receiving cavity 11;

[0053] The cover body 2 is detachably covered on the open end of the box body 1 to form a sealed accommodation cavity 11; wherein, two mounting holes 21 communicating with the accommodation cavity 11 are formed on the cover body 2; for example, the cover body 2 and the box body 1 are connected by threads; that is, internal threads are formed on the cover body 2, external threads are formed on the outer peripheral wall of the box body 1, and the internal threads are adapted to the external threads; preferably, in order to improve the connection sealing performance between the box body 1 and the cover body 2, a sealing ring can be arranged between them; the box body 1 and the cover body 2 adopt a separable connection design, and a special sealing ring is arranged at the connection to ensure that they can form a sealed experimental space to simulate the soil respiration process under different environmental conditions. Of course, the present utility model is not limited thereto. There are glue sealing tapes, buckles and other structures on the cover body 2. After being connected to the box body 1, a fully enclosed space is obtained by closing the buckle.

[0054] The syringe 5 is communicated with the accommodation cavity 11 through one of the mounting holes 21 and is configured to inject culture liquid into the accommodation cavity 11;

[0055] The gas collection tube 6 has one end communicated with the other mounting hole 21 and the other end communicated with the gas detection component.

[0056] Investigate the sampling site of the soil to be measured, master environmental characteristics such as precipitation and temperature, measure the volume of the box body 1, and count the volume of the soil to be cultured, the volume of the alkali solution, etc. (ignoring the volume occupied by the materials of the containers for containing the alkali solution and the soil), and calculate the volume of the air in the sealed box during cultivation; Put the soil sample 200 transported back by refrigeration into the box body 1 according to different requirements (small amount: 0 - 100 g, medium amount: 10 - 500 g, large amount: 500 - 1000 g, and extra-large amount: more than 1000 g), adjust the temperature of the incubator or the indoor air to the appropriate temperature of 25 °C and culture for 3 d. During this period, water or nutrient solution can be added as needed. Wait until the soil is in the state at the time of sampling before starting the sealed culture; During the sealed culture, use the syringe 5 to inject liquid into the accommodation cavity 11, and it is also necessary to always keep the water full during the culture to avoid the entry of air and reduce the systematic error of the data; Collect the discharged gas in the accommodation cavity 11 through the gas collection tube 6, and calculate the respiration rate of the soil by the gas detection component;

[0057] The detection device 100 allows for multi-batch and independent cultivation of soil samples 200, and supports accurate determination of soil respiration rates under different land use conditions. The materials of this device are easy to obtain and the cost is low, making it convenient for large-scale experimental applications. It can not only freely adjust the temperature and humidity in the experiment to better simulate natural conditions, but also effectively avoid the interference of meteorological and regional influencing factors, providing a new experimental tool for soil carbon cycle and global climate change research. In addition, the usage method of this device is simple, providing an effective technical means for studying the effects of different land use patterns and soil layers on soil respiration rates.

[0058] In an example of the present utility model, it further includes: at least one valve 3,

[0059] which is installed on at least one of the two mounting holes 21, and is configured to control the injection amount of the culture liquid in the syringe 5 and / or the discharge amount of the gas in the accommodation chamber 11;

[0060] For example, the valve 3 is fixedly and sealingly connected in the mounting hole 21 by hot melt adhesive; by arranging the valve 3 in the mounting hole 21 connected to the syringe 5, the injection amount of the culture liquid in the syringe 5 can be controlled, and at the same time, by arranging the valve 3 in the mounting hole 21 connected to the gas discharge pipe, the discharge amount of the gas in the accommodation chamber 11 can be controlled; this is beneficial to the control of the detection device 100 and reduces pollution and errors during the detection process.

[0061] In an example of the present utility model, the valve 3 is a piston air extraction joint;

[0062] For example, the valve 3 is a curved piston air extraction joint, and the curved piston air extraction joint and the cover body 2 are connected through the mounting hole 21. Among them, a part of the curved piston air extraction joint is placed below the sealing cover for the input of moisture or nutrient solution, or the output of CO 2 gas, and the part containing the piston is placed above the sealing cover to facilitate the control of the input of moisture and the output of gas. The rubber tube 4 is used for the sealing connection of the curved piston air extraction joint 3 with the syringe 5 and the gas collection tube 6, which can effectively remove the errors caused by the mixing of air.

[0063] For example, the valve 3 is made of polytetrafluoroethylene material and has a straight or curved piston air extraction joint design, which can effectively control the flow of gas and liquid and reduce pollution and errors during the experiment.

[0064] Of course, the present utility model is not limited to this. The valve 3 can also be other valve body structures such as a flow valve or a on-off valve, as long as it can achieve the control of the flow rate.

[0065] In an example of the present utility model, a rubber tube 4 is also connected between the syringe 5 and the valve 3 and between the gas collection tube 6 and the valve 3;

[0066] By providing a rubber tube 4 for sealing connection between the valve 3 and the syringe 5, and for sealing connection between the valve 3 and the gas collection tube 6, errors caused by air mixing can be effectively eliminated.

[0067] In one example of the present utility model, the culture liquid includes tap water, deionized water or a compound nutrient solution.

[0068] In one example of the present utility model, the box body 1 includes a transparent plastic part, a translucent plastic part or an opaque plastic part;

[0069] The box body 1 is made of transparent, translucent or opaque plastic to meet different experimental requirements, allowing researchers to select the most suitable type of box body 1 according to the experimental purpose.

[0070] In one example of the present utility model, the gas detection assembly includes: a gas chromatograph or an infrared carbon dioxide detector 10;

[0071] Specifically, when the gas detection assembly is a gas chromatograph, as Figure 1 shown, the instrument (gas chromatograph) is used to measure the concentration of CO 2 in the gas discharged from the accommodation chamber 11 (for example, the concentration of CO 2 in the gas collected by the collection bag described below), and further calculate the volume of CO 2 released by the culture soil according to the total volume of the gas in the packaging box body 1, and calculate the respiration rate of the soil sample 200.

[0072] As Figure 2 shown, when using the infrared carbon dioxide detector 10 method, only need to connect the infrared carbon dioxide detector 10 to the gas collection tube 6. After it is connected to the accommodation chamber 11, record the change of time and the CO 2 concentration. When the index on the carbon dioxide detector tends to be stable, it is regarded as the completion of the culture. The respiration rate of the soil sample 200 can be calculated according to the time and the CO 2 concentration.

[0073] In one example of the present utility model, it further includes: a gas collection bag 7,

[0074] which is connected to the gas collection tube 6 and is used to collect the gas discharged from the mounting hole 21 and place it in the gas detection assembly for detection;

[0075] When detecting the respiration rate of the soil sample 200 by a gas chromatograph, it is inconvenient to detect the gas discharged from the accommodation chamber 11. By providing the gas collection bag 7, the gas discharged from the accommodation chamber 11 can be effectively collected, facilitating the detection of CO2 The concentration is measured.

[0076] In an example of the present utility model, the cover body 2 and the box body 1 are connected by threads. An external thread is provided on the outer edge of the box body 1, and an internal thread adapted to the external thread is formed inside the cover body 2. Wherein, a sealing ring is further provided between the cover body 2 and the box body 1; that is, a sealing ring is also built in the bottom of the cover body 2. When the cover body 2 is adapted to the box body 1, the sealing ring is compacted between the cover body 2 and the box body 1.

[0077] Of course, the present utility model is not limited thereto. The cover body 2 and the box body 1 can also be connected by snap connection. For example, a snap is pivotally connected to the cover body 2, and a protrusion adapted to the snap is formed on the box body 1. When it is necessary to fixedly seal the connection between the cover body 2 and the box body 1, the snap is rotated to fixedly connect it to the protrusion through friction; preferably, a plurality of snaps are provided and arranged at intervals along the circumferential direction of the cover body 2. Correspondingly, the protrusions and the snaps correspond one by one; it can be understood that a sealing ring is also provided on the cover body 2 to ensure a sealed connection between the cover body 2 and the box body 1 during the connection process.

[0078] A detection device 100 for simulating soil respiration rate according to the second aspect of the present utility model, as Figure 3 shown, includes:

[0079] A box body 1, forming a receiving cavity 11 with an open end. Wherein, a soil sample 200 is placed in the receiving cavity 11;

[0080] A cover body 2, detachably covering the open end of the box body 1 to form a sealed receiving cavity 11; wherein, an installation hole 21 communicating with the receiving cavity 11 is provided on the cover body 2;

[0081] A syringe 5, which is communicated with the receiving cavity 11 through the installation hole 21 and is configured to inject a culture liquid into the receiving cavity 11;

[0082] A beaker 8, containing an alkaline solution inside, and is arranged in the receiving cavity 11 and above the soil sample 200.

[0083] As Figure 3As shown, the detection device 100 uses the alkali solution absorption acid-base titration method to conduct research on the sampling site of the soil to be measured, master environmental characteristics such as precipitation and temperature, measure the volume of the measuring box body 1, and count the volume of the soil to be cultivated, the volume of the alkali solution, etc. (ignoring the volume occupied by the materials of the containers for containing the alkali solution and the soil), and calculate the volume of the air in the sealed box during cultivation; for the soil sample 200 transported back by refrigeration, according to different requirements (small amount: 0 - 100 g, medium amount: 10 - 500 g, large amount: 500 - 1000 g, and extra-large amount: more than 1000 g), put it into the box body 1, adjust the temperature of the incubator or the indoor temperature to the appropriate temperature of 25 °C and cultivate for 3 days. During this period, culture liquid (such as water or nutrient solution) can be added as needed. Wait until the soil is in the state at the time of sampling before starting sealed cultivation; during sealed cultivation, use a syringe 5 to inject liquid into the accommodating cavity 11, and it is also necessary to always keep the moisture full during cultivation to avoid air entry and reduce the systematic error of the data; place a beaker 8 containing a standard concentration of quantitative alkali solution (for absorbing the released CO 2 ) on the soil sample 200. During the cultivation process, the CO 2 released by soil respiration is completely absorbed by the alkali solution, then take out the container containing the alkali solution, and use the acid-base titration method to measure the content of CO 2 absorbed by the alkali solution.

[0084] In an example of the present utility model, it further includes: a valve 3,

[0085] which is installed in the installation hole 21 and configured to control the injection amount of the culture liquid in the syringe 5.

[0086] By arranging the valve 3 in the installation hole 21 connected to the syringe 5, the injection amount of the culture liquid in the syringe 5 can be controlled; this is beneficial to the control of the detection device 100 and reduces pollution and errors during the detection process.

[0087] For example, the valve 3 is a curved piston air extraction joint. The curved piston air extraction joint and the cover body 2 are connected through the installation hole 21. Among them, a part of the curved piston air extraction joint is placed below the sealing cover for the input of water or nutrient solution, or the output of CO 2 gas. The part containing the piston is placed above the sealing cover to facilitate the control of the input of water and the output of gas. The rubber tube 4 is used to seal and connect the curved piston air extraction joint 3 to the syringe 5, which can effectively remove the error caused by air mixing.

[0088] In an example of the present utility model, the culture liquid includes tap water, deionized water or compound nutrient solution.

[0089] In an example of the present utility model, it further includes: a backing plate 9,

[0090] It is laid on the soil sample 200, and the beaker 8 is placed on the upper end of the backing plate 9;

[0091] By providing the backing plate 9, the beaker 8 can be placed flat, preventing the beaker 8 from tilting or toppling, thereby affecting the detection accuracy of the detection device 100.

[0092] It can be understood that for a detection device 100 for simulating soil respiration rate according to the present utility model, the following should be noted in the detection laboratory:

[0093] (1) According to the experimental requirements, the incubator or room temperature can be adjusted to the average temperature of the simulated location for cultivation. When the soil respiration characteristics tend to be stable, a 6 - 8 - hour closed cultivation can be carried out, and then a gas collection bag 7 for collecting CO 2 is used for measurement;

[0094] (2) According to the experimental requirements, the box body 1 is filled with soils of different depths and different soil layers alternately, and different climate conditions and soil humidity levels under different soil layers can be simulated through the syringe 5;

[0095] (3) First, create a suitable temperature (25°C) through an incubator, etc., cultivate the frozen - preserved soil sample 200 for 3 days, and then adjust the temperature to the average temperature of the required simulated environment. After that, according to the soil usage, carry out closed cultivation for 1h, 4h, 8h, 12h, 24h, etc., and then cooperate with the humidity to construct the soil respiration CO 2 release rate.

[0096] Specifically, the detection method of a detection device 100 for simulating soil respiration rate according to the present utility model includes the following steps:

[0097] S10: According to the research plan, calculate the number of required detection devices 100, prepare the corresponding number of detection devices 100, and conduct research on the sampling site of the soil to be measured to master environmental characteristics such as precipitation and temperature;

[0098] S20: Clean the experimental box body 1, the modified cover body 2, the rubber tube 4, etc., and place them for a certain period of time to remove other gases on the surface of the plastic material to ensure that the experiment is not affected;

[0099] S30: Measure the internal volume of the box body 1, count the volume of the soil to be cultivated, the volume of the alkali solution, etc. (ignoring the volume occupied by the materials of the containers for holding the alkali solution and the soil), and calculate the volume of the air in the box body 1 during cultivation;

[0100] S40: Put the soil sample 200 transported back by refrigerated transportation into the box body 1 according to different demand amounts (small amount: 0 - 100 g, medium amount: 10 - 500 g, large amount: 500 - 1000 g, and extra large amount: more than 1000 g), adjust the temperature of the incubator or the indoor temperature to the appropriate temperature of 25 °C and cultivate for 3 days. During this period, water or nutrient solution can be added as needed. Wait until the soil is in the state at the time of sampling before starting the sealed cultivation;

[0101] S50: During sealed cultivation, pay attention to connecting the curved piston air extraction joint to the subsequent structure corresponding to it before sealing. On the basis of tightening the piston, fill the water of one side piston air extraction joint with a syringe 5, and also keep the water full at all times during cultivation to avoid air entry and reduce the systematic error of data;

[0102] S60: As Figure 1 shown, if the method of collecting gas to measure the concentration is adopted, there is no need to place alkali solution. Just close the piston on the air extraction joint, then remove the gas collection bag 7, and use an instrument (gas chromatography) to measure the concentration of CO 2 in the gas collection bag, and further calculate the volume of CO 2 released by the cultivated soil according to the total volume of the gas in the accommodation cavity 11 to calculate the respiration rate;

[0103] As Figure 2 shown, if the infrared CO 2 detection instrument method is adopted, only after connection, record the time and the change of the CO 2 concentration. When the index on the instrument tends to be stable, it is regarded as the completion of cultivation, and the calculation can be carried out according to the time and the CO 2 concentration;

[0104] As Figure 3 shown, if the alkali solution absorption acid-base titration method is adopted, another beaker 8 containing a standard concentration of quantitative alkali solution (for absorbing the released CO 2 ) should be placed on the cushion wood. During the cultivation process, the CO 2 released by soil respiration is completely absorbed by the alkali solution, then take out the container containing the alkali solution, and use the acid-base titration method to measure the content of CO 2 absorbed by the alkali solution.

[0105] In the above text, the exemplary embodiments of the detection device 100 for simulating soil respiration rate proposed by the present utility model are described in detail with reference to the preferred embodiments. However, those skilled in the art can understand that without departing from the concept of the present utility model, various modifications and variations can be made to the above specific embodiments, and various combinations can be made to the various technical features and structures proposed by the present utility model, without exceeding the protection scope of the present utility model. The protection scope of the present utility model is determined by the appended claims.

Claims

1. A detection device for simulating soil respiration rate, characterized in that: include: The box body (1) is formed with a receiving cavity (11) having an open end, wherein a soil sample (200) is placed in the receiving cavity (11); A cover body (2) is detachably covered on the open end of the box body (1) to form a closed accommodating cavity (11); wherein the cover body (2) is provided with two mounting holes (21) which are in communication with the accommodating cavity (11); a syringe (5), which is connected to the accommodating chamber (11) through one of the mounting holes (21) and is configured to inject culture liquid into the accommodating chamber (11); A gas collecting pipe (6) has one end connected to another of the mounting holes (21) and the other end connected to the gas detection assembly.

2. The detection device for simulating soil respiration rate according to claim 1, characterized in that: Also includes: at least one valve (3), It is mounted on at least one of the two mounting holes (21) and is configured to control the injection amount of the culture liquid in the syringe (5) and / or the discharge amount of the gas in the containing chamber (11).

3. The detection device for simulating soil respiration rate according to claim 2, characterized in that: A rubber hose (4) is also connected between the injector (5) and the valve (3) as well as between the gas collecting pipe (6) and the valve (3).

4. The detection device for simulating soil respiration rate according to claim 1, characterized in that: The culture liquid includes tap water, deionized water or compound nutrient solution.

5. The detection device for simulating soil respiration rate according to claim 1, characterized in that: The box body (1) comprises a transparent plastic part, a translucent plastic part or an opaque plastic part.

6. The detection device for simulating soil respiration rate according to claim 1, characterized in that: The gas detection component comprises: a gas chromatograph or an infrared carbon dioxide detector (10).

7. The detection device for simulating soil respiration rate according to claim 1, characterized in that: Also includes: a gas collection bag (7), It is connected to the gas collection pipe (6) and is used to collect the gas discharged from the installation hole (21) and place it in the gas detection component for detection.

8. A detection device for simulating soil respiration rate, characterized in that: include: The box body (1) is formed with a receiving cavity (11) having an open end, wherein a soil sample (200) is placed in the receiving cavity (11); A cover body (2) is detachably covered on the open end of the box body (1) to form a closed accommodating cavity (11); wherein the cover body (2) is provided with a mounting hole (21) which is in communication with the accommodating cavity (11); a syringe (5), which is connected to the accommodating chamber (11) through the mounting hole (21) and is configured to inject culture liquid into the accommodating chamber (11); A beaker (8) containing an alkaline solution is disposed in the containing cavity (11) and is located on the soil sample (200).

9. The detection device for simulating soil respiration rate according to claim 6, characterized in that: Also includes: a valve (3), It is installed in the installation hole (21) and is configured to control the injection amount of the culture liquid in the syringe (5).

10. The detection device for simulating soil respiration rate according to claim 6, characterized in that: The culture liquid includes tap water, deionized water or compound nutrient solution.