Decoloration kettle used in L-carnosine enzymatic synthesis

Abstract

A discharging barrel is arranged at the bottom of a kettle body, a liquid outlet and an activated carbon recovery port are respectively arranged on the discharging barrel, a filter cartridge is arranged in the liquid outlet, a piston block is movably arranged in the discharging barrel, a concentrated solution can be firstly discharged from the discharging port through the piston block, and the activated carbon recovery port is arranged at the bottom of the kettle body. Activated carbon is left in the discharging barrel through the filter cylinder, after concentrated liquid is discharged, the piston block continues to descend, the activated carbon can be discharged from the activated carbon recycling opening, a filtering device does not need to be additionally arranged, cost and convenience are greatly improved, and meanwhile the recycling efficiency of the activated carbon is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of decolorization reactors, and specifically relates to a decolorization kettle used in the enzymatic synthesis of L-carnosine. Background Art

[0002] L-carnosine, also known as β-alanyl-L-histidine, has the molecular formula: C9H14N4O3 and a molecular weight of 226.23. It is a dipeptide obtained by condensing two amino acids, β-alanine and L-histidine, and is a crystalline solid. It was first discovered in beef in 1900 and is widely present in the brains, muscles and other tissues of mammals. It is a naturally active dipeptide.

[0003] L-carnosine has antioxidant and anti-aging functions. It has therapeutic effects on hypertension, heart disease, senile cataract, ulcers, etc., and also has biological activities such as anti-tumor. Adding carnosine during the storage of fresh meat can improve the flavor of the meat and extend the shelf life. L-carnosine has been proven to scavenge reactive oxygen species (ROS) and α-β unsaturated aldehydes formed by the excessive oxidation of fatty acids in cell membranes during oxidative stress. Adding carnosine to cosmetics can delay skin aging. Compared with other antioxidants, L-carnosine has the advantages of strong antioxidant ability, no toxic side effects, and multiple physiological activities, and has broad application prospects in the fields of medicine, health care, hygiene, beauty, etc. At present, the exploration of new physiological activities and mechanisms of L-carnosine remains a hot topic.

[0004] The steps of the enzymatic synthesis of L-carnosine are: strain transformation, enzyme fermentation culture, cell separation, enzyme immobilization, enzyme catalysis, separation and purification, membrane concentration, decolorization, crystallization, centrifugal drying. Among them, the concentrated retentate after membrane concentration is transferred into a decolorization kettle, and decolorization treatment is carried out in the decolorization kettle by adding activated carbon. After decolorization, crystallization and centrifugal drying are carried out to obtain L-carnosine.

[0005] At present, after the decolorization is completed with the cooperation of activated carbon in the decolorization kettle used in the enzymatic synthesis of L-carnosine, the activated carbon and the concentrated solution are discharged together into a separate filtration device. After filtration, the concentrated solution and the activated carbon are separated. The concentrated solution is separated and transferred into a crystallization kettle, and the activated carbon is recycled. However, this structure requires a separate filtration device on the one hand, increasing the cost, and on the other hand, filtering through the filtration device reduces the production efficiency. Therefore, an improved technology is urgently needed to solve this problem existing in the prior art. Summary of the Utility Model

[0006] The purpose of the present utility model is to provide a decolorization kettle used in the enzymatic synthesis of L-carnosine. The concentrated liquid can be first discharged from the discharge port through the piston block, and the activated carbon is left in the discharge cylinder body through the filter cylinder. After the concentrated liquid is discharged, the piston block continues to descend, and the activated carbon can be discharged from the activated carbon recovery port. There is no need to additionally set up a filtering device, which greatly improves the cost and convenience, and at the same time improves the recovery efficiency of the activated carbon, so as to solve the problems raised in the above background technology.

[0007] To achieve the above purpose, the present utility model provides the following technical solution: A decolorization kettle used in the enzymatic synthesis of L-carnosine, including a kettle body, a kettle cover, a reduction motor, a stirrer main shaft, a stirring paddle, an activated carbon cylinder, a hydraulic cylinder, a liquid outlet and an activated carbon recovery port. The upper surface of the kettle body is provided with a kettle cover. The center position of the upper surface of the kettle cover is provided with a motor seat. The upper surface of the motor seat is provided with a reduction motor. The output shaft of the reduction motor is connected to the top end of the stirrer main shaft through a coupling. The bottom end of the stirrer main shaft is provided with a stirring paddle. The stirring paddle is rotatably arranged inside the kettle body. The upper surface of the kettle cover is also provided with a liquid inlet and an activated carbon addition port. The upper surface of the activated carbon addition port is installed with an activated carbon cylinder through an electric valve. The outside of the kettle body is provided with a jacket. The bottom of the kettle body is provided with a discharge cylinder body. The bottom of the discharge cylinder body is provided with a hydraulic cylinder through a connecting plate. The piston rod of the hydraulic cylinder is installed with a piston block. The piston block is slidably arranged inside the discharge cylinder body. One side of the discharge cylinder body is provided with a liquid outlet. The other side of the discharge cylinder body and obliquely below the liquid outlet is provided with an activated carbon recovery port. A filter cylinder is arranged inside the liquid outlet.

[0008] Preferably, in the decolorization kettle used in the enzymatic synthesis of L-carnosine provided by the present utility model, the top of the piston block is arched, and the outer diameter of the piston block is the same as the inner diameter of the discharge cylinder body.

[0009] Preferably, in the decolorization kettle used in the enzymatic synthesis of L-carnosine provided by the present utility model, the upper surface of the kettle cover is also provided with a thermometer port, a pressure gauge port, a protective gas inlet and a protective gas outlet.

[0010] Preferably, in the decolorization kettle used in the enzymatic synthesis of L-carnosine provided by the present utility model, the lower part of the jacket is provided with a steam inlet, and one side of the upper part of the jacket is provided with a steam outlet.

[0011] Preferably, in the decolorization kettle used in the enzymatic synthesis of L-carnosine provided by the present utility model, a plurality of support seats are evenly arranged on the outer surface of the jacket.

[0012] Preferably, in the decolorization kettle used in the enzymatic synthesis of L-carnosine provided by the present utility model, a through hole is opened at the center position of the kettle cover, and a sealing ring is arranged on the lower surface of the kettle cover at the through hole, and the stirrer main shaft is matched with the sealing ring.

[0013] Preferably, a decolorization kettle used in the enzymatic synthesis of L-carnosine provided by the present utility model, wherein one end of the filter cylinder is open and provided with an outer folding plate, the outer folding plate is provided with a plurality of through holes, the other end of the filter cylinder is provided with a plurality of filter holes, and the outer diameter of the filter cylinder is consistent with the inner diameter of the liquid outlet.

[0014] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0015] A discharge cylinder body is arranged at the bottom of the kettle body. The discharge cylinder body is respectively provided with a liquid outlet and an activated carbon recovery port. A filter cylinder is arranged inside the liquid outlet. A piston block is movably arranged inside the discharge cylinder body. The concentrated liquid can be discharged from the discharge port through the piston block first, and the activated carbon is left in the discharge cylinder body through the filter cylinder. When the concentrated liquid is discharged, the piston block continues to descend, and the activated carbon can be discharged from the activated carbon recovery port. There is no need to additionally arrange a filtering device, which greatly improves the cost and convenience, and at the same time improves the recovery efficiency of the activated carbon. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic cross-sectional structure diagram of the present utility model;

[0017] Figure 2 is a schematic external structure diagram of the present utility model;

[0018] Figure 3 is a schematic cross-sectional structure diagram of the filter cylinder;

[0019] Figure 4 is a schematic front view structure diagram of the filter cylinder.

[0020] In the figure: kettle body 1, kettle cover 2, reduction motor 3, stirrer main shaft 4, stirring paddle 5, activated carbon cylinder 6, hydraulic cylinder 7, liquid outlet 8, activated carbon recovery port 9, motor base 10, liquid inlet 11, activated carbon addition port 12, electric valve 13, jacket 14, discharge cylinder body 15, piston block 16, filter cylinder 17, thermometer port 18, pressure gauge port 19, protective gas inlet 20, protective gas outlet 21, steam inlet 22, steam outlet 23, support seat 24, sealing ring 25, outer folding plate 26, filter hole 27. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] Next, the technical solutions of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model;

[0022] It should be noted that in the description of the present utility model, the orientation or positional relationship indicated by terms such as "inner", "outer", "upper", "lower", "both sides", "one end", "the other end", "left", "right", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model.

[0023] Please refer to Figures 1-4, the present utility model provides a technical solution: a decolorization kettle used in the enzymatic synthesis of L-carnosine, comprising a kettle body 1, a kettle cover 2, a reduction motor 3, a stirrer main shaft 4, a stirrer paddle 5, an activated carbon cartridge 6, a hydraulic cylinder 7, a liquid outlet 8 and an activated carbon recovery port 9. The upper surface of the kettle body 1 is provided with a kettle cover 2. The center position of the upper surface of the kettle cover 2 is provided with a motor base 10. The upper surface of the motor base 10 is provided with a reduction motor 3. The output shaft of the reduction motor 3 is connected to the top end of the stirrer main shaft 4 through a coupling. The bottom end of the stirrer main shaft 4 is provided with a stirrer paddle 5. The stirrer paddle 5 is rotatably arranged inside the kettle body 1. A through hole is opened at the center position of the kettle cover 2. A sealing ring 25 is arranged on the lower surface of the kettle cover 2 and at the position of the through hole. The stirrer main shaft 4 is matched with the sealing ring 25 to ensure the sealing performance at the position of the stirrer main shaft 4 and the kettle cover 2. The upper surface of the kettle cover 2 is also provided with a liquid inlet 11 and an activated carbon addition port 12. The upper surface of the activated carbon addition port 12 is installed with an activated carbon cartridge 6 through an electric valve 13. The upper surface of the kettle cover 2 is also provided with a thermometer port 18, a pressure gauge port 19, a protective gas inlet 20 and a protective gas outlet 21. The thermometer port 18 is used for installing a thermometer. The pressure gauge port 19 is used for installing a pressure gauge. The protective gas inlet 20 and the protective gas outlet 21 are used for the entry and discharge of nitrogen. A jacket 14 is arranged outside the kettle body 1. A steam inlet 22 is arranged at the lower part of the jacket 14. A steam outlet 23 is arranged at one side of the upper part of the jacket 14 to realize the entry and discharge of high-temperature steam into and out of the jacket 14. A plurality of support seats 24 are evenly arranged on the outer surface of the jacket 14 to support the decolorization kettle, so as to support the decolorization kettle on the platform. The bottom of the kettle body 1 is provided with a discharge cylinder body 15. The bottom of the discharge cylinder body 15 is provided with a hydraulic cylinder 7 through a connecting plate. The piston rod of the hydraulic cylinder 7 is installed with a piston block 16. The piston block 16 is slidably arranged inside the discharge cylinder body 15. The top of the piston block 16 is arched. The arched structure avoids the accumulation of activated carbon. The outer diameter of the piston block 16 is the same as the inner diameter of the discharge cylinder body 15 to ensure that the concentrated liquid will not leak out between the discharge cylinder body 15 and the piston block 16. A liquid outlet 8 is arranged at one side of the discharge cylinder body 15. An activated carbon recovery port 9 is obliquely arranged at the other side of the discharge cylinder body 15 and below the liquid outlet 8. A filter cartridge 17 is arranged inside the liquid outlet 8. One end of the filter cartridge 17 is open and provided with an outer folding plate 26. A plurality of through holes are opened in the outer folding plate 26. The outer folding plate 26 is used for the limit of the filter cartridge 17. The through holes of the outer folding plate 26 are used for the bolts to pass through when the liquid outlet 8 is connected to the external pipeline. The other end of the filter cartridge 17 is provided with a plurality of filter holes 27. The outer diameter of the filter cartridge 17 is the same as the inner diameter of the liquid outlet 8. The activated carbon is blocked through the filter holes 27, and the concentrated liquid can be smoothly discharged.

[0024] Installation method and operating principle: First, install the reduction motor 3 on the upper surface of the motor base 10. Then, pass the top of the agitator main shaft 4 with the agitator paddle 5 through the sealing ring 25 and connect it to the output shaft of the reduction motor 3 through a coupling. Next, place the agitator paddle 5 into the kettle body 1 by a crane, and fix the kettle cover 2 on the upper surface of the kettle body 1 with bolts. Then, fix the kettle body 1 at the platform through the support base 24, connect the piston block 16 to the piston rod of the hydraulic cylinder 7, insert the piston block 16 into the discharge cylinder body 15, and install the hydraulic cylinder 7 at the connecting plate at the bottom of the discharge cylinder body 15. Finally, insert the filter cartridge 17 into the liquid outlet 8 to complete the installation. Before use, connect the liquid outlet pipe to the flange at the outer end of the liquid outlet 8 through a flange. At this time, the outer folding plate 26 of the filter cartridge 17 is clamped between the liquid outlet 8 and the flange of the liquid outlet pipe. Connect the steam inlet pipe to the steam inlet 22, connect the steam discharge pipe to the steam outlet 23, install the thermometer and pressure gauge at the thermometer port 18 and the pressure gauge port 19 respectively, connect the nitrogen inlet pipe to the protective gas inlet 20, connect the nitrogen discharge pipe to the protective gas outlet 21, install the activated carbon cartridge 6 on the upper surface of the activated carbon addition port 12 through the electric valve 13, and connect the ultrafiltration membrane device to the liquid inlet 11 through a pipe to complete the connection with the external pipes. During use, make the top of the piston block 16 flush with the bottom inside the kettle body 1 through the hydraulic cylinder 7. The retentate obtained by the ultrafiltration membrane device enters the kettle body 1 from the liquid inlet 11 through a pipe. At the same time, add activated carbon into the kettle body 1, then add nitrogen into the kettle body 1 through the protective gas inlet 20, add high-temperature steam into the jacket 14 from the steam inlet 22, and keep the temperature inside the kettle body 1 at 40 - 60 °C. Start the reduction motor 3, and the reduction motor 3 drives the agitator paddle 5 to rotate through the agitator main shaft 4, so as to perform decolorization treatment on the concentrated solution of L-carnosine. After 30 - 60 minutes, the reduction motor 3 decelerates or stops, and the hydraulic cylinder 7 drives the piston block 16 to descend to the activated carbon recovery port 9. The upper end of the piston block 16 is aligned with the bottom of the liquid outlet 8, and the decolorized concentrated solution is discharged from the liquid outlet 8. Then, the piston block 16 continues to descend, and the upper end of the piston block 16 is lower than the bottom of the activated carbon recovery port 9, and the activated carbon is discharged from the activated carbon recovery port 9 and recycled. The structure of the present utility model is reasonable. The bottom of the kettle body 1 is provided with a discharge cylinder body 15, the discharge cylinder body 15 is respectively provided with a liquid outlet 8 and an activated carbon recovery port 9, a filter cartridge 17 is arranged in the liquid outlet 8, and a piston block 16 is movably arranged inside the discharge cylinder body 15. The concentrated solution can be discharged from the discharge port by the piston block 16 first, and the activated carbon is left in the discharge cylinder body 15 through the filter cartridge 17. When the concentrated solution is discharged, the piston block 16 continues to descend, and the activated carbon can be discharged from the activated carbon recovery port 9, without additionally arranging a filtering device, greatly improving the cost and convenience, and at the same time improving the recovery efficiency of the activated carbon.

[0025] For those parts not detailed in the present utility model, they are all well-known technologies to those skilled in the art.

[0026] Finally, it should be noted that the above specific embodiments are only used to illustrate the technical solutions of the present invention rather than to limit them. Although the present invention has been described in detail with reference to the embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified and equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered by the scope of the claims of the present invention.

Claims

1. A decolorizing kettle used in the enzymatic synthesis of L-carnosine, characterized in that: The invention comprises a kettle body (1), a kettle cover (2), a reduction motor (3), a stirrer main shaft (4), a stirring paddle (5), an activated carbon barrel (6), a hydraulic cylinder (7), a liquid outlet (8) and an activated carbon recovery port (9), wherein the upper surface of the kettle body (1) is provided with a kettle cover (2), a motor seat (10) is provided at the center position of the upper surface of the kettle cover (2), a reduction motor (3) is provided on the upper surface of the motor seat (10), an output shaft of the reduction motor (3) is connected to the top of the stirrer main shaft (4) through a coupling, a stirring paddle (5) is provided at the bottom end of the stirrer main shaft (4), and the stirring paddle (5) is rotatably arranged inside the kettle body (1), and a liquid inlet (11) and an activated carbon adding port (9) are also provided on the upper surface of the kettle cover (2). The kettle body (1) is provided with a charging port (12), an activated carbon material cylinder (6) is installed on the upper surface of the activated carbon charging port (12) through an electric valve (13), a jacket (14) is provided on the outside of the kettle body (1), a discharge cylinder (15) is provided on the bottom of the kettle body (1), a hydraulic cylinder (7) is provided on the bottom of the discharge cylinder (15) through a connecting plate, a piston block (16) is installed on the piston rod of the hydraulic cylinder (7), and the piston block (16) is slidably arranged in the discharge cylinder (15), a liquid outlet (8) is provided on one side of the discharge cylinder (15), an activated carbon recovery port (9) is obliquely provided on the other side of the discharge cylinder (15) and below the liquid outlet (8), and a filter cartridge (17) is provided inside the liquid outlet (8).

2. The decolorizing kettle used in the enzymatic synthesis of L-carnosine according to claim 1, characterized in that: The top of the piston block (16) is arched, and the outer diameter of the piston block (16) is consistent with the inner diameter of the discharge cylinder (15).

3. The decolorizing kettle used in the enzymatic synthesis of L-carnosine according to claim 1, characterized in that: The upper surface of the kettle cover (2) is also provided with a thermometer port (18), a pressure gauge port (19), a protective gas inlet (20) and a protective gas outlet (21).

4. The decolorizing kettle used in the enzymatic synthesis of L-carnosine according to claim 1, characterized in that: The lower part of the jacket (14) is provided with a steam inlet (22), and one side of the upper part of the jacket (14) is provided with a steam outlet (23).

5. The decolorizing kettle used in the enzymatic synthesis of L-carnosine according to claim 1, characterized in that: A plurality of support seats (24) are evenly arranged on the outer surface of the jacket (14).

6. The decolorizing kettle used in the enzymatic synthesis of L-carnosine according to claim 1, characterized in that: A through hole is provided at the center of the kettle cover (2), a sealing ring (25) is provided on the lower surface of the kettle cover (2) and located at the through hole, and the stirrer main shaft (4) cooperates with the sealing ring (25).

7. The decolorizing kettle used in the enzymatic synthesis of L-carnosine according to claim 1, characterized in that: One end of the filter cartridge (17) is open and provided with an outer folded plate (26), the outer folded plate (26) is provided with a plurality of through holes, and the other end of the filter cartridge (17) is provided with a plurality of filter holes (27), and the outer diameter of the filter cartridge (17) is consistent with the inner diameter of the liquid outlet (8).

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