Experimental microwell plate and microwell plate frame

Abstract

The utility model relates to the technical field of microwell plates, and discloses an experimental microwell plate and a microwell plate frame, which are mainly suitable for observation and counting of single molecule detection by a microscope, and specifically comprise two end plates, a panel and a flat plate, the number of the end plates is two, the panel is arranged between the two end plates and is vertical to each other, and the flat plate is arranged between the two end plates. A plurality of sample adding holes distributed in the length direction of the panel are formed in the panel, sample adding pipes are installed at openings in the bottom ends of the sample adding holes, and the flat plate is installed at the openings in the bottom ends of the sample adding pipes in a sealed mode and is parallel to the panel. According to the utility model, the openings at the bottom ends of the plurality of sample adding pipes are simultaneously blocked by the same flat plate, so that the bottom ends of the plurality of sample adding pipes are flush, the problem that the bottoms of the independently blocked sample adding pipes are not in the same plane is avoided, continuous shooting of a plurality of samples is facilitated, and meanwhile, the imaging precision under continuous shooting is improved, so that the accuracy of an experimental result is improved; therefore, the requirement of single-molecule detection is met.

Description

Technical Field

[0001] The utility model relates to the technical field of microplates, in particular to an experimental microplate and a microplate rack. Background Technique

[0002] When observing and counting by using a microscope for single molecule detection, a microplate is mostly used as a sample adding carrier. The microplate usually has 6, 12, 24, 48, 96 and 384 sample holes, and is applicable to almost all applications in life science research, including filtration, separation, optical detection, storage, reaction mixing, cell culture and detection of antibacterial activity.

[0003] At present, most of the existing microplates and microplate racks are of traditional structural designs. An array structure of holes corresponding to the microplate is arranged on the microplate rack. The microplate is inserted and placed on the supporting microplate rack from top to bottom. Limited by the hole structure on the microplate rack, only a single glass slide can be used at the bottom of the sample adding tube on the microplate, which may lead to different heights. When the experimental lens takes continuous photos, the phenomenon of lens jamming may occur, and then the shooting accuracy is relatively low, affecting the accuracy of the actual experimental results and not meeting the requirements of single molecule detection. Content of the Utility Model

[0004] To solve the technical problems existing in the background technique, the utility model provides an experimental microplate and a microplate rack.

[0005] An experimental microplate provided by the utility model is mainly applicable to the observation and counting of single molecule detection by a microscope. Specifically, it includes end plates, a panel and a flat plate. The number of end plates is two and they are arranged oppositely. The panel is installed between the two end plates and is perpendicular to them. A plurality of sample adding holes distributed along the length direction of the panel are opened on the panel. A sample adding tube is installed at the bottom opening of the sample adding hole. The flat plate is hermetically installed at the bottom opening of the plurality of sample adding tubes and is parallel to the panel.

[0006] As a further optimized scheme of the utility model, it further includes connecting bars. The connecting bars are installed between the two end plates and are arranged parallel to the lower part of the panel. The number of connecting bars is two and they are arranged oppositely. The flat plate is arranged between the two connecting bars.

[0007] As a further optimized scheme of the utility model, the flat plate is a transparent plate with a thickness of 0.08 - 0.25 mm.

[0008] An experimental microplate rack, which is used in combination with the above experimental microplate. Specifically, it includes a frame. A plurality of spaced sliders are arranged on the side part of the frame. The microplate is slidably sleeved in the sliders. A limiting block is arranged at the opening of the slider to limit and stabilize the microplate in the slider.

[0009] As a further optimized solution of the present utility model, a partition is installed inside the frame. The number of partitions is multiple and they are spaced along the length direction of the frame. Two adjacent partitions are parallel to each other to form a slideway.

[0010] As a further optimized solution of the present utility model, slide rails are installed on the opposite surfaces of two adjacent partitions. Slide grooves adapted to the slide rails are provided on both sides of the microplate. The microplate is slidably inserted between the two partitions through the slide grooves.

[0011] As a further optimized solution of the present utility model, the frame is a square box body with an open top, and a transparent cover plate that can be opened outward is installed at the open top.

[0012] The experimental microplate and the microplate rack proposed by the present utility model have the following beneficial effects:

[0013] (1) By simultaneously blocking the bottom openings of multiple sampling tubes with the same flat plate, the bottoms of the multiple sampling tubes are made flush, avoiding the problem that the bottoms of independently blocked sampling tubes are not in the same plane, which is beneficial to the continuous shooting of multiple samples, and at the same time improving the imaging accuracy under continuous shooting, thereby improving the accuracy of experimental results to meet the requirements of single molecule detection;

[0014] (2) By installing multiple partitions spaced inside the frame, two adjacent partitions form a slideway adapted to the microplate, so that the microplate can be slidably inserted into the slideway from the opening on the side of the frame, and then the opening of the slideway is blocked by a limit block, avoiding the limitation of the traditional hole position array structure, facilitating the placement and removal of the microplate in a sliding and extracting manner, and being beneficial to improving the actual use convenience.

[0015] The additional aspects and advantages of the present utility model will be partially given in the following description, partially become obvious from the following description, or be understood through the practice of the present utility model. Description of the Drawings

[0016] Figure 1 is a three-dimensional structural schematic diagram of the microplate of the present utility model;

[0017] Figure 2 is a three-dimensional structural schematic diagram of the microplate rack of the present utility model;

[0018] Figure 3 is a front sectional structural schematic diagram of the microplate rack of the present utility model;

[0019] Figure 4 is of the present utility model Figure 3 in which is a front sectional structural schematic diagram of the microplate.

[0020] Description of the drawings: 1. End plate; 2. Panel; 3. Connecting bar; 4. Sampling hole; 5. Sampling tube; 6. Flat plate; 7. Frame; 8. Slideway; 9. Limit block; 10. Transparent cover plate; 11. Partition; 12. Slide rail. Detailed implementation

[0021] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar symbols represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present invention and should not be construed as a limitation to the present invention.

[0022] It should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention.

[0023] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present invention, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0024] In the present invention, unless otherwise clearly specified and defined, the terms "installed", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection, an electrical connection, or communicable with each other; it may be directly connected, or indirectly connected through an intermediate medium, and may be the internal communication of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] In the present utility model, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0026] As Figure 1 shown, an experimental microplate is mainly applicable to the observation and counting of single molecule detection by a microscope, and specifically includes end plates 1, a panel 2, and a flat plate 6. The number of end plates 1 is two and they are arranged oppositely. The panel 2 is installed between the two end plates 1 and is perpendicular to each other. A plurality of sample addition holes 4 are formed in the panel 2 and are distributed along its length direction. A sample addition tube 5 is installed at the bottom opening of each sample addition hole 4. The flat plate 6 is hermetically installed at the bottom openings of the plurality of sample addition tubes 5 and is parallel to the panel 2.

[0027] Specifically, it further includes connecting bars 3. The connecting bars 3 are installed between the two end plates 1 and are arranged parallel to each other below the panel 2. The number of connecting bars 3 is two and they are arranged oppositely. The flat plate 6 is arranged between the two connecting bars 3 so that the lens of the microscope can extend between the two connecting bars 3 and be in contact with the bottom surface of the flat plate 6 for subsequent imaging experiments.

[0028] The connecting bars 3 are arranged below the panel 2. The two end plates 1, the panel 2, and the two connecting bars 3 surround each other to form a rectangular frame structure. The plurality of sample addition tubes 5 are evenly arranged along the length direction of the rectangular frame, thereby forming a complete microplate structure. The bottom openings of the plurality of sample addition tubes 5 of the microplate are blocked by the same flat plate 6, so that the bottom openings of the plurality of sample addition tubes 5 are flush, avoiding different heights, thereby facilitating continuous and uninterrupted shooting by the subsequent experimental lens and avoiding lens jamming.

[0029] Furthermore, the flat plate 6 is a transparent plate with a thickness of 0.08 - 0.25 mm, which can be a glass plate or a transparent plastic plate. Here, it is preferably a borosilicate glass plate, which has the characteristics of high light transmittance and low fluorescence background, facilitating observation and counting during the experiment.

[0030] As Figure 2As shown in the figure, an experimental microplate rack is used in conjunction with the above-mentioned experimental microplate. Specifically, it includes a frame 7. Multiple spaced slides 8 are provided on the side of the frame 7. The microplate is slidably sleeved in the slide 8. A limit block 9 is provided at the opening of the slide 8 to limit and stabilize the microplate in the slide 8. By providing multiple slides 8, it is convenient to slide multiple microplates into the frame 7 and also convenient to individually access a certain microplate;

[0031] Specifically, one end of the limit block 9 is rotatably installed on the side of the frame 7 through a damping rotating shaft and is located outside the slide 8. The other end of the limit block 9 can rotate and move to the opening of the slide 8, and the inner side surface of the limit block 9 abuts against the end plate 1 of the microplate, so as to lock and limit the microplate in the slide 8;

[0032] Specifically, as Figure 3 shown in the figure, a partition 11 is installed inside the frame 7. The number of partitions 11 is multiple and they are spaced along the length direction of the frame 7. Two adjacent partitions 11 are parallel to each other to form the slide 8;

[0033] Furthermore, as Figure 4 shown in the figure, slide rails 12 are installed on the opposite surfaces of two adjacent partitions 11. Sliding grooves adapted to the slide rails 12 are provided on both sides of the microplate. The microplate is slidably inserted between the two partitions 11 through the sliding grooves, and the sliding grooves are slidably connected to the slide rails, so as to facilitate pulling the microplate outwards or pushing it inwards from the side of the frame 7;

[0034] Specifically, the frame 7 is a square box body with an open top, and a transparent cover plate 10 that can be opened outwards is installed at the open top. The transparent cover plate 10 can be installed at the open top of the frame 7 by means of hinge, or can also be directly placed and fixed with fasteners, which can play a role in dust prevention and avoiding cross-contamination;

[0035] Furthermore, multiple observation ports are provided at the bottom of the frame 7. The number of observation ports is the same as and corresponds one by one to the number of slides 8. Each observation port is respectively communicated with the corresponding slide 8 inside, so that the microscope lens can extend upwards from the bottom to between the two connecting strips 3, thus facilitating imaging experiments on the flat plate 6 and the samples thereon;

[0036] It should be noted that there are also types of ordinary microplate racks with dust-proof cover plates at present. However, when accessing the microplate, the cover plate must be completely opened or partially opened, and then the microplate is taken out from the microplate rack from bottom to top, which will pose a risk of foreign objects falling into the microplate and contaminating the sample. The combination of the above-mentioned microplate and microplate rack, the method of sliding out from the side can solve the above problems;

[0037] In summary, for the experimental microplate and the microplate holder proposed by the present utility model, the bottom openings of multiple sample addition tubes are simultaneously blocked by the same flat plate, making the bottoms of the multiple sample addition tubes flush, avoiding the problem that the bottoms of the independently blocked sample addition tubes are not in the same plane, facilitating the continuous shooting of multiple samples, improving the imaging accuracy under continuous shooting, thereby improving the accuracy of experimental results to meet the requirements of single molecule detection; by installing a plurality of spacers 11 distributed at intervals inside the frame 7, adjacent two spacers 11 form a slideway 8 adapted to the microplate, enabling the microplate to be slidably inserted into the slideway 8 from the opening on the side of the frame 7, and then the opening of the slideway 8 is blocked by the limit block 9, avoiding the limitation of the traditional hole position array structure, facilitating the placement and removal of the microplate in a sliding and extracting manner, and being beneficial to improving the practical use convenience.

[0038] The above is only the preferred specific embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution and the inventive concept of the present utility model, making equivalent substitutions or changes, shall be covered by the protection scope of the present utility model.

Claims

1. An experimental microplate, characterized in that: The invention comprises an end plate (1), a panel (2) and a flat plate (6); the number of the end plates (1) is two and they are arranged opposite to each other; the panel (2) is installed between the two end plates (1) and they are perpendicular to each other; the panel (2) is provided with a plurality of sample addition holes (4) distributed along its length direction; a sample addition tube (5) is installed at the bottom opening of the sample addition hole (4); and the flat plate (6) is sealed and installed at the bottom opening of the plurality of sample addition tubes (5) and is parallel to the panel (2).

2. An experimental microplate according to claim 1, characterized in that: It also includes a connecting bar (3), which is installed between the two end plates (1) and arranged in parallel below the panel (2). There are two connecting bars (3) which are arranged opposite to each other, and the flat plate (6) is arranged between the two connecting bars (3).

3. An experimental microplate according to claim 1, characterized in that: The flat plate (6) is a transparent plate with a thickness of 0.08-0.25 mm.

4. An experimental microplate rack, used in conjunction with an experimental microplate according to any one of claims 1 to 3, characterized in that: The invention comprises a frame (7), a plurality of slideways (8) distributed at intervals are arranged on the side of the frame (7), a micro-porous plate is slidably mounted in the slideway (8), a limiting block (9) is arranged at the opening of the slideway (8), and the micro-porous plate in the slideway (8) is limited and stabilized by the limiting block (9).

5. An experimental microplate rack according to claim 4, characterized in that: A partition (11) is installed inside the frame (7). The partitions (11) are multiple in number and are spaced apart along the length direction of the frame (7). Two adjacent partitions (11) are parallel to each other to form a slideway (8).

6. An experimental microplate rack according to claim 5, characterized in that: The opposite surfaces of two adjacent partitions (11) are both provided with slide rails (12), and both sides of the microporous plate are provided with slide grooves adapted to the slide rails (12), and the microporous plate is slidably inserted between the two partitions (11) through the slide grooves.

7. An experimental microplate rack according to claim 4, characterized in that: The frame (7) is a square box body with an opening on the top, and a transparent cover plate (10) which can be opened outwards is installed at the top opening.

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