Production device of all-component culture medium

Abstract

The utility model discloses a production device of an all-component culture medium, and relates to the technical field of corn deep processing devices, settling clear liquid of a corn soaking water tank is communicated with a reaction tank, and an inlet of the reaction tank is respectively communicated with a basic metal salt tank and a calcium chloride tank; an outlet of the reaction tank is communicated with a filter, a liquid phase outlet of the filter is communicated with an enzymolysis tank, an inlet of the enzymolysis tank is communicated with a compound enzyme agent tank, an outlet of the enzymolysis tank is communicated with an enzymatic hydrolysate tank, an outlet of the enzymatic hydrolysate tank is communicated with a first concentration tank through a pipeline, and an inlet of the first concentration tank is communicated with a carbon source tank; an outlet of the first concentration tank is communicated with a dryer through a pipeline; an outlet of the dryer is communicated with a product tank through a pipeline. The product is stable in character, not easy to absorb moisture and cake, and convenient to store and use; the ash content is low, and the growth of microorganisms cannot be inhibited; the carbon-nitrogen ratio is reasonable, and the problems of high nitrogen and low carbon of conventional corn steep liquor dry powder are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of corn deep processing devices, and particularly relates to a production device for a whole-component culture medium. Background Art

[0002] Corn steep liquor is a by-product generated in the process of wet production of corn starch. The main components not only include basic nutrients such as proteins, amino acids, and phytic acid that meet the growth requirements of microorganisms, but also contain factors such as vitamins and trace elements that induce the production of secondary metabolites, making corn steep liquor an ideal nutrient source for the fermentation industry.

[0003] At present, corn steep liquor is mostly processed into liquid corn syrup or solid corn syrup dry powder for sale. Liquid corn syrup is prone to fermentation and deterioration, resulting in loss of nutrients, and the transportation distance cost is also high; solid corn syrup dry powder solves the problems of easy deterioration of corn syrup, high transportation cost, and short shelf life, and has a wide range of application scenarios. However, the corn syrup or corn syrup dry powder produced by traditional processes has the following defects: high acidity, which affects the growth of microorganisms and causes slow fermentation; high ash content, especially high potassium element content, which limits the growth and metabolism of microorganisms; low carbon-nitrogen ratio, and additional carbon source needs to be supplemented during use. Summary of the Invention

[0004] The technical problem to be solved by the utility model is: aiming at the deficiencies existing in the prior art, to provide a production device for a whole-component culture medium, and the obtained whole-component culture medium has low acidity and ash content, high carbon-nitrogen ratio, and balanced nutrition.

[0005] To solve the above technical problem, the technical solution of the utility model is:

[0006] A production device for a whole-component culture medium includes a corn steep liquor tank. The outlet of the corn steep liquor tank is connected to a sedimentation tank through a pipeline. The liquid phase outlet of the sedimentation tank is connected to a clear liquid tank through a pipeline. The outlet of the clear liquid tank is connected to a reaction tank through a pipeline. The inlet of the reaction tank is respectively connected to an alkali metal salt tank and a calcium chloride tank through pipelines;

[0007] The outlet of the reaction tank is connected to a filter through a pipeline. The liquid phase outlet of the filter is connected to an enzymolysis tank through a pipeline. The inlet of the enzymolysis tank is connected to a compound enzyme agent tank through a pipeline. The outlet of the enzymolysis tank is connected to an enzymolysis liquid tank through a pipeline. The outlet of the enzymolysis liquid tank is connected to a first concentrator through a pipeline. The inlet of the first concentrator is connected to a carbon source tank through a pipeline. The outlet of the first concentrator is connected to a dryer through a pipeline. The outlet of the dryer is connected to a product tank through a pipeline.

[0008] As an improved technical solution, the solid phase outlet of the filter is connected to a phytic acid calcium tank.

[0009] As an improved technical solution, the outlet of the enzymatic hydrolysate tank is connected to a nanofiltration membrane device through a pipeline, the retentate outlet of the nanofiltration membrane device is connected to a retentate tank through a pipeline, and the outlet of the retentate tank is connected to the first concentration tank through a pipeline.

[0010] As an improved technical solution, the permeate outlet of the nanofiltration membrane device is connected to a permeate tank through a pipeline, the outlet of the permeate tank is connected to a second concentration tank through a pipeline, and the outlet of the second concentration tank is connected to a lactate tank through a pipeline.

[0011] As an improved technical solution, the molecular weight cut-off of the nanofiltration membrane device is 100 - 200 Da.

[0012] As an improved technical solution, the dryer is a spray dryer.

[0013] Due to the adoption of the above technical solution, the beneficial effects of the present utility model are as follows:

[0014] A production device for a full-component culture medium of the present utility model includes a corn steep water tank. The outlet of the corn steep water tank is connected to a sedimentation tank through a pipeline. The liquid-phase outlet of the sedimentation tank is connected to a clear liquid tank through a pipeline. The outlet of the clear liquid tank is connected to a reaction tank through a pipeline. The inlet of the reaction tank is respectively connected to an alkaline metal salt tank and a calcium chloride tank through pipelines. The outlet of the reaction tank is connected to a filter through a pipeline. The liquid-phase outlet of the filter is connected to an enzymatic hydrolysis tank through a pipeline. The inlet of the enzymatic hydrolysis tank is connected to a compound enzyme agent tank through a pipeline. The outlet of the enzymatic hydrolysis tank is connected to an enzymatic hydrolysate tank through a pipeline. The outlet of the enzymatic hydrolysate tank is connected to a first concentration tank through a pipeline. The inlet of the first concentration tank is connected to a carbon source tank through a pipeline. The outlet of the first concentration tank is connected to a dryer through a pipeline. The outlet of the dryer is connected to a product tank through a pipeline. After the corn steep water is statically sedimented in the sedimentation tank for a period of time, the suspended sediment and other foreign matters are discharged from the bottom, the clear liquid is collected and put into the reaction tank. Then, an alkaline metal salt is added to the reaction tank to adjust the pH of the system to 5 - 6, and then calcium chloride is added to promote the complexation of sulfite ions and partial phytic acid ions in the system with calcium salts to form precipitates. After filtering to remove the precipitates, the filtrate is collected and placed into the enzymatic hydrolysis tank, and through reaction with a compound enzyme preparation of cellulase and hemicellulase, the polysaccharide substances in the system are degraded, the viscosity of the feed liquid is reduced, which is beneficial to improving the nanofiltration speed and reducing the moisture absorption phenomenon during the drying process. The enzymatic hydrolysate enters the first concentration tank, is mixed and dissolved with carbon sources such as glucose, maltodextrin or starch, and then the dissolved liquid is concentrated, and finally enters the dryer. After drying, the finished product is obtained. The product has stable properties, is not easy to absorb moisture and caking, and is convenient for storage and use; it has a low ash content and will not inhibit the growth of microorganisms; the carbon-nitrogen ratio is reasonable, solving the problem of high nitrogen and low carbon of conventional corn steep liquor dry powder.

[0015] The solid-phase outlet of the filter of the present utility model is connected to a phytic acid calcium tank, and phytic acid calcium can be sold externally, improving the economic benefits of the product.

[0016] The outlet of the enzyme hydrolysis liquid tank is connected to a nanofiltration membrane device through a pipeline. The retentate outlet of the nanofiltration membrane device is connected to a retentate tank through a pipeline. The outlet of the retentate tank is connected to the first concentration tank through a pipeline. The nanofiltration membrane device can remove excess potassium ions in the feed liquid, avoid the inhibition of the growth and metabolism of microorganisms due to excessive potassium ion concentration, and at the same time can remove some small-molecule organic impurities such as lactic acid, reducing the caking phenomenon after product drying.

[0017] The permeate outlet of the nanofiltration membrane device is connected to a permeate tank through a pipeline. The outlet of the permeate tank is connected to a second concentration tank through a pipeline. The outlet of the second concentration tank is connected to a lactate tank through a pipeline. Lactate can be sold externally, avoiding treatment as hazardous waste, improving the economic value of the product, and being environmentally friendly.

[0018] The cut-off molecular weight of the nanofiltration membrane device is 100 - 200 Da, and it has a good filtering effect on small-molecule organic impurities such as potassium ions and lactic acid.

[0019] The dryer is a spray dryer, which has a good drying effect and shortens the drying cycle. Description of the Drawings

[0020] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0021] Figure 1 is a schematic structural diagram of an embodiment of the present utility model;

[0022] Wherein: 1, corn steep water tank; 2, sedimentation tank; 3, clear liquid tank; 4, reaction tank; 5, basic metal salt tank; 6, calcium chloride tank; 7, filter; 8, enzyme hydrolysis tank; 9, compound enzyme agent tank; 10, enzyme hydrolysis liquid tank; 11, first concentration tank; 12, carbon source tank; 13, dryer; 14, product tank; 15, phytic acid calcium tank; 16, nanofiltration membrane device; 17, retentate tank; 18, permeate tank; 19, second concentration tank; 20, lactate tank. Detailed Embodiments

[0023] The present utility model will be further elaborated below in conjunction with the drawings and embodiments.

[0024] As Figure 1As shown in the figure, a production device for a complete-component culture medium includes a corn steep water tank 1. The outlet of the corn steep water tank 1 is connected to a sedimentation tank 2 through a pipeline. The liquid-phase outlet of the sedimentation tank 2 is connected to a clear liquid tank 3 through a pipeline. The outlet of the clear liquid tank 3 is connected to a reaction tank 4 through a pipeline. The inlet of the reaction tank 4 is respectively connected to an alkali metal salt tank 5 and a calcium chloride tank 6 through pipelines. The outlet of the reaction tank 4 is connected to a filter 7 through a pipeline. The liquid-phase outlet of the filter 7 is connected to an enzymatic hydrolysis tank 8 through a pipeline. The inlet of the enzymatic hydrolysis tank 8 is connected to a compound enzyme agent tank 9 through a pipeline. The outlet of the enzymatic hydrolysis tank 8 is connected to an enzymatic hydrolysate tank 10 through a pipeline. The outlet of the enzymatic hydrolysate tank 10 is connected to a first concentration tank 11 through a pipeline. The inlet of the first concentration tank 11 is connected to a carbon source tank 12 through a pipeline. The outlet of the first concentration tank 11 is connected to a dryer 13 through a pipeline. The outlet of the dryer 13 is connected to a product tank 14 through a pipeline. After the corn steep water is statically sedimented in the sedimentation tank 2 for a period of time, the suspended sediment and other foreign matters are discharged from the bottom, the clear liquid is collected and put into the reaction tank 4, and then an alkali metal salt is added to the reaction tank 4 to adjust the pH of the system to 5-6. The alkali metal salt in this embodiment is one of sodium hydroxide or potassium hydroxide, which promotes the complexation of sulfite ions and part of phytic acid ions in the clear liquid with calcium salts to form precipitates. After filtering to remove the precipitates, the filtrate is collected and placed into the enzymatic hydrolysis tank 8, and through reaction with a compound enzyme preparation of cellulase and hemicellulase, the polysaccharide substances in the system are degraded, the viscosity of the feed liquid is reduced, which is beneficial to improving the nanofiltration speed and reducing the moisture absorption phenomenon during the drying process. The enzymatic hydrolysate enters the first concentration tank 11, is mixed and dissolved with carbon sources such as glucose, maltodextrin or starch, and then the dissolved solution is concentrated, and finally enters the dryer 13. After drying, the finished product is obtained. The product has stable properties, is not easy to absorb moisture and caking, and is convenient for storage and use; it has low ash content and will not inhibit the growth of microorganisms; the carbon-nitrogen ratio is reasonable, solving the problem of high nitrogen and low carbon of conventional corn steep liquor dry powder.

[0025] The solid-phase outlet of the filter 7 is connected to a phytic acid calcium tank 15. Phytic acid calcium can be sold externally, improving the economic benefits of the product.

[0026] The outlet of the enzymatic hydrolysate tank 10 is connected to a nanofiltration membrane device 16 through a pipeline. The retentate outlet of the nanofiltration membrane device 16 is connected to a retentate tank 17 through a pipeline. The outlet of the retentate tank 17 is connected to the first concentration tank 11 through a pipeline. The nanofiltration membrane device 16 can remove the excess potassium ions in the feed liquid, avoid the inhibition of the growth and metabolism of microorganisms due to too high potassium ion concentration, and at the same time can remove some small-molecule organic impurities such as lactic acid, reducing the caking phenomenon after product drying.

[0027] The permeate outlet of the nanofiltration membrane device 16 is connected to a permeate tank 18 through a pipeline. The outlet of the permeate tank 18 is connected to a second concentration tank 19 through a pipeline. The outlet of the second concentration tank 19 is connected to a lactate tank 20 through a pipeline. The lactate can be sold externally, avoiding treatment as hazardous waste, improving the economic value of the product, and being environmentally friendly.

[0028] The nanofiltration membrane device 16 has a retention molecular weight of 100 - 200 Da and has a good filtering effect on small molecule organic impurities such as potassium ions and lactic acid.

[0029] The dryer is a spray dryer, which has a good drying effect and shortens the drying cycle.

[0030] It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of this application.

Claims

1. A production device for a full-component culture medium, characterized in that: It comprises a corn soaking water tank, the outlet of the corn soaking water tank is connected to a sedimentation tank through a pipeline, the liquid phase outlet of the sedimentation tank is connected to a clear liquid tank through a pipeline, the outlet of the clear liquid tank is connected to a reaction tank through a pipeline, and the inlet of the reaction tank is connected to an alkaline metal salt tank and a calcium chloride tank through pipelines respectively; The outlet of the reaction tank is connected to a filter through a pipeline, the liquid phase outlet of the filter is connected to an enzymolysis tank through a pipeline, the inlet of the enzymolysis tank is connected to a composite enzyme tank through a pipeline, the outlet of the enzymolysis tank is connected to an enzymolysis liquid tank through a pipeline, the outlet of the enzymolysis liquid tank is connected to a first concentration tank through a pipeline, the inlet of the first concentration tank is connected to a carbon source tank through a pipeline, the outlet of the first concentration tank is connected to a dryer through a pipeline, and the outlet of the dryer is connected to a product tank through a pipeline.

2. A production device for a full-component culture medium according to claim 1, characterized in that: The solid phase outlet of the filter is communicated with a calcium phytate tank.

3. The production device of a full-component culture medium according to claim 1, characterized in that: The outlet of the enzymatic hydrolyzate tank is connected to a nanofiltration membrane device via a pipeline, the intercepted liquid outlet of the nanofiltration membrane device is connected to an intercepted liquid tank via a pipeline, and the outlet of the intercepted liquid tank is connected to the first concentration tank via a pipeline.

4. A production device for a full-component culture medium as claimed in claim 3, characterized in that: The permeate outlet of the nanofiltration membrane device is connected to a permeate tank via a pipeline, the outlet of the permeate tank is connected to a second concentration tank via a pipeline, and the outlet of the second concentration tank is connected to a lactate tank via a pipeline.

5. The production device of a full-component culture medium according to claim 3, characterized in that: The molecular weight cut-off of the nanofiltration membrane device is 100-200Da.

6. The production device of a full-component culture medium according to claim 1, characterized in that: The dryer is a spray dryer.

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