Portable potato in-vitro leaf inoculation fungal disease resistance identification device

Abstract

The utility model relates to the field of agricultural equipment, in particular to a portable potato in-vitro leaf inoculation fungal disease resistance identification device which comprises an outer box, an inner box, an inoculation measurement observation plate, a water absorption layer and a top box, the outer box and the top box are opaque, and the inner box and the inoculation measurement observation plate are transparent; the water absorption layer is laid in the outer box, and the inner box is placed in the outer box; the interior of the inner box is divided into a plurality of rows of grids, each row of grids are vertically through, one part of one grid in each row of grids is used for pasting a label, and the other part of the grid is used as a water injection port; supporting rods are fixed in the other grids in each row of grids; the inoculation measurement observation plate is placed on the inner box, grid scale marks corresponding to the grids are arranged on the inoculation measurement observation plate, and small holes corresponding to the grids are formed in the inoculation measurement observation plate; the top box covers the outer box, and a temperature control device, a light source and an ultraviolet sterilizing lamp are arranged on the inner top surface of the top box. The method can overcome the regional limitation of inoculation tests, and is suitable for tests of different scales.

Description

Technical Field

[0001] This application relates to the field of agricultural equipment, and particularly to a portable device for identifying the resistance of potato detached leaves to fungal diseases by inoculation. Background Art

[0002] In the research on potatoes, it is usually necessary to measure the resistance level of potato varieties to fungal diseases (such as potato late blight, early blight, etc.). Specifically, a bacterial suspension is used to inoculate potato detached leaves, and in vitro culture is carried out by maintaining appropriate temperature and light duration. Then, by observing the disease occurrence of the leaves and measuring the lesion size, the resistance level of potato varieties to fungal diseases is identified.

[0003] Currently, the inoculation test of potato detached leaves can only be carried out in an artificial climate chamber or an artificial climate incubator, which results in limitations in the test location. In addition, artificial climate chambers or artificial climate incubators are more suitable for large-scale tests, while small-scale tests waste resources and are prone to pollution.

[0004] Therefore, how to overcome the geographical limitations of the inoculation test of potato detached leaves, and also be able to adapt to inoculation tests of different scales, avoiding resource waste and environmental pollution, is a technical problem that needs to be urgently solved by those skilled in the art at present. Summary of the Utility Model

[0005] This application provides a portable device for identifying the resistance of potato detached leaves to fungal diseases by inoculation, so as to overcome the geographical limitations of the inoculation test of potato detached leaves, and also be able to adapt to inoculation tests of different scales, avoiding resource waste and environmental pollution.

[0006] To solve the above technical problems, this application provides the following technical solutions:

[0007] A portable device for identifying the resistance of potato detached leaves to fungal diseases by inoculation includes: an outer box, an inner box, an inoculation measurement and observation board, a water absorption layer, and a top box. The outer box and the top box are opaque, and the inner box and the inoculation measurement and observation board are transparent; the water absorption layer is laid in the outer box, and the inner box is placed in the outer box and is located above the water absorption layer; the interior of the inner box is divided into multiple rows of squares, each row of squares penetrates up and down, and a part of one square in each row of squares is used for pasting labels, and the remaining part of this square is used as a water injection port; the remaining squares in each row of squares are fixed with support rods; the inoculation measurement and observation board is placed on the inner box, and the inoculation measurement and observation board has grid scale lines corresponding to each square, and small holes corresponding to each square are opened on the inoculation measurement and observation board; the top box is covered on the outer box, and the inner top surface of the top box is provided with a temperature control device, a light source, and an ultraviolet sterilization lamp.

[0008] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, there are two opposite handles on the side of the outer box.

[0009] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, there are opposite outer box buckles on the side of the outer box, and the outer box buckles snap the top box to the upper part of the outer box.

[0010] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, the square for pasting labels and serving as a water injection port in each row of squares is the leftmost square.

[0011] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, each square has three support rods, and the three support rods are evenly arranged in the same plane and are located near the bottom of the square.

[0012] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, a fixing clip is clamped on one support rod.

[0013] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, the inoculation measurement and observation board also has digital scales.

[0014] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, there are three LED light sources evenly distributed on the inner top surface of the top box; there are two temperature control devices on the inner top surface of the top box, and each temperature control device is distributed between two LED light sources; there is an ultraviolet sterilization lamp on the inner top surface of the top box, and the ultraviolet sterilization lamp is located between one temperature control device and the middle LED light source.

[0015] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, the outer surface of the top box has a display screen for displaying the temperature, humidity, light time, and ultraviolet lamp irradiation time inside the top box; the outer surface of the top box has a control panel for controlling the on and off of the temperature control device, LED light source, and ultraviolet sterilization lamp.

[0016] The portable device for identifying the resistance of potato detached leaves to fungal diseases as described above, wherein, preferably, the outer surface of the top box has a charging or plugging port; the charging or plugging port is connected to a battery, and the battery is connected to the temperature control device, light source, ultraviolet sterilization lamp, display screen, and control panel.

[0017] Compared with the above background art, the portable device for identifying the resistance of potato detached leaves to fungal diseases in this application can be used by charging or plugging in, which is convenient to carry. After the leaves are collected, tests can be carried out in time or the leaves can be preserved, ensuring the activity of the leaves, improving the accuracy of the tests, and overcoming the geographical limitations of the tests. Moreover, the portable device for identifying the resistance of potato detached leaves to fungal diseases provided in this application can also adapt to inoculation tests of different scales, avoiding waste of resources and environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings described below are only some embodiments recorded in this application. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings.

[0019] Figure 1 is a perspective view of the portable device for identifying the resistance of potato detached leaves to fungal diseases provided by the embodiment of this application;

[0020] Figure 2 is an assembly schematic diagram of the outer box and the inner box provided by the embodiment of this application;

[0021] Figure 3 is an exploded schematic diagram of the outer box and the inner box provided by the embodiment of this application;

[0022] Figure 4 is a partial enlarged view of the inner box provided by the embodiment of this application;

[0023] Figure 5 is a schematic diagram of the inoculation measurement and observation board provided by the embodiment of this application;

[0024] Figure 6 is an internal schematic diagram of the top cover provided by the embodiment of this application;

[0025] Figure 7 is an external schematic diagram of the top cover provided by the embodiment of this application. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] The following details the embodiments of this application. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. In addition, spatial relationship terms such as "upper", "lower", "left", "right", "front", "rear", etc. are used to facilitate the description to explain the positional relationship between two components. The embodiments described below by referring to the drawings are exemplary and are only used to explain this application and should not be construed as a limitation of this application.

[0027] AsFigure 1 , Figure 2 , Figure 3 and Figure 4 As shown in Figure 1 , Figure 2 , Figure 3 and Figure 4 , the present application provides a portable device for identifying the resistance of potato in vitro leaves to fungal diseases, including: an outer box 110, an inner box 120, an inoculation measurement and observation board 130, a water absorption layer (not shown in the figure), and a top box 140; wherein, the outer box 110 and the top box 140 are made of opaque materials, and the inner box 120 and the inoculation measurement and observation board 130 are made of transparent materials.

[0028] The water absorption layer is laid in the outer box 110. Optionally, the water absorption layer is a filter paper and absorbent cotton layer. Further optionally, the side of the outer box 110 has two opposite handles 111, which are convenient for the operator to hold the outer box 110 with both hands. Additionally optionally, the cross-sectional shape of the outer box 110 is square. Still optionally, the side of the outer box 110 also has opposite outer box buckles 112 for clamping the top box 140 to the upper part of the outer box 110. Still optionally, there are two outer box buckles 112 on one side of the outer box 110, and there are also two outer box buckles 112 on the opposite side of the outer box 110 to ensure the clamping effect. Specifically, the lower end of the outer box buckle 112 is hinged to the side of the outer box 110, and by rotating the outer box buckle 112, the upper end of the outer box buckle 112 presses on the upper surface of the top box 140 to complete the clamping.

[0029] The inner box 120 is placed in the outer box 110 and is located above the water absorption layer; the interior of the inner box 120 is divided into multiple rows of squares 121, each row of squares 121 penetrates up and down, and a part of one square 121 in each row of squares 121 is used for pasting a label, and the label is used to record the situation of the leaves in that row of squares. The remaining part of this square 121 is used as a water injection port, and sterile water is injected through the water injection port so that the water absorption layer can adsorb an appropriate amount of sterile water to ensure humidity; in each row of squares 121, the remaining squares 121 are fixed with support rods 122 for supporting the leaves, avoiding the leaves from directly contacting the bottom water absorption layer and rotting, and effectively separating the leaves through the squares 121 of the inner box 120, which can reduce the mutual influence and contamination between samples.

[0030] Optionally, the square 121 in each row of squares 121 for pasting the label and serving as the water injection port is the leftmost square 121. Further optionally, the length of the square 121 for pasting the label and serving as the water injection port is less than the length of the square 121 for holding the leaves. Additionally optionally, the shape of each square 121 for holding the leaves is a cube. Still optionally, there are 10 squares 121 for holding the leaves in each row of squares 121, and there are 10 rows of squares 121. In addition, in order to facilitate pasting the label, the top of the square 121 for pasting the label and serving as the water injection port has a top plate that closes a part of this square 121, and the label can be pasted on this top plate.

[0031] Optionally, each square 121 has three support rods 122, and the three support rods 122 are evenly arranged in the same plane. Optionally, the support rods 122 are located at the position of the square 121 close to the bottom to avoid interfering with the leaves. Optionally, a fixing clip 123 is clamped on one support rod 122 for clamping the petiole, so as to make the main vein centered as much as possible, effectively fix the position of the leaf, reduce the inoculation deviation, and facilitate observation and measurement. Optionally, the fixing clip 123 can be movably clamped onto the support rod 122 to facilitate adjusting the position to adapt to petioles of different shapes. Optionally, two fixing clips 123 are clamped on one support rod 122.

[0032] The inoculation measurement and observation plate 130 is placed on the inner box 120. The inoculation measurement and observation plate 130 has grid scale lines 131 corresponding to each square 121 for observing and measuring the size of the lesions on the leaves located in the corresponding square 121. And small holes 132 corresponding to each square 121 are formed on the inoculation measurement and observation plate 130, and the bacterial liquid is inoculated into the leaves in the square 121 through the small holes 132. Through the inoculation measurement and observation plate 130, the inoculation position can be conveniently controlled and marked, the size of the lesions can be measured quickly and accurately, and the test accuracy can be improved.

[0033] Optionally, the grid scale lines 131 have horizontal scale lines and vertical scale lines, and the horizontal scale lines and vertical scale lines intersect to form the grid scale lines 131. Optionally, the inoculation measurement and observation plate 130 also has digital scales (not shown in the figure) for facilitating the observation of the size of the lesions. Optionally, the small holes 132 are located at the positions corresponding to the centers of the squares 121.

[0034] The top box 140 is covered on the outer box 110, and the inner top surface of the top box 140 has a temperature control device 141, a light source 142 and an ultraviolet sterilization lamp 143. The ultraviolet sterilization lamp 143 is used for ultraviolet sterilization before and after each test, so as to reduce pollution. The light source 140 and the temperature control device 141 are used for providing suitable temperature and light during each test.

[0035] Optionally, the light source 142 is an LED light source, which can be an LED lamp tube or an LED light strip. Optionally, three LED light sources are evenly distributed on the inner top surface of the top box 140. Optionally, the inner top surface of the top box 140 has two temperature control devices 141 (for example: electric heating tube rods), and each temperature control device 141 is distributed between two LED light sources to provide temperature more evenly. Optionally, the inner top surface of the top box 140 has an ultraviolet sterilization lamp 143, and the ultraviolet sterilization lamp 143 is located between one temperature control device 141 and the middle LED light source, so that the ultraviolet sterilization lamp 143 is located in the middle position as much as possible, which is beneficial to sterilizing each position.

[0036] Optionally, the outer surface of the set-top box 140 has a display screen 144. The display screen 144 is used to display the temperature, humidity, light exposure time, and ultraviolet lamp irradiation time inside the set-top box 140. For the measurement of temperature and humidity, it can be measured by sensors. For the measurement of light exposure time and ultraviolet lamp irradiation time, it can be measured by timers. These are all existing conventional technical means. Optionally, the outer surface of the set-top box 140 has a control panel 145. Through the control panel 145, the temperature control device 141, the light source 142, and the ultraviolet sterilization lamp 143 can be controlled to turn on and off. For controlling the turning on and off of electronic devices (the temperature control device 141, the light source 142, and the ultraviolet sterilization lamp 143), it is also an existing conventional technical means. Optionally, the outer surface of the set-top box 140 also has a charging or plugging port 146; the charging or plugging port 146 is connected to a battery (not shown in the figure). The battery is connected to the temperature control device 141, the light source 142, the ultraviolet sterilization lamp 143, the display screen 144, and the control panel 145 to provide the required electrical energy for the temperature control device 141, the light source 142, the ultraviolet sterilization lamp 143, the display screen 144, and the control panel 145.

[0037] When using the portable potato detached leaf inoculation fungal disease resistance identification device provided by the present application for experiments, first collect the healthy small leaves in the upper middle part of the potato plants at the full flowering stage. Put the small leaves with intact petioles into the corresponding grids 121 of the transparent inner box 120. The leaves are supported by three support rods 122 at the bottom of the grid 121. The petioles are inserted into the water-absorbing layer to effectively moisturize the leaves. The movable fixing clips 123 on the support rods 122 are used to fix the petioles, and try to place the main vein in the center. Then, prepare a pathogen spore suspension with a certain concentration, and quantitatively inoculate the middle part of the leaf in the grid 121 near the main vein through the small holes on the inoculation measurement and observation board 130, which is convenient for inoculation at the same position, convenient for later measurement and investigation, and reduces deviation. Then adjust to the appropriate temperature and light exposure time for the pathogen to develop, and incubate for 7 - 10 days. During this period, add sterile water from the water injection port according to the humidity monitoring to ensure the required humidity. During this period, the lesion characteristics can be observed using the inoculation measurement and observation board 130, and the cross method can be used to measure and count the lesion diameter, and the disease resistance of the potato variety to the pathogen can be classified and identified according to the disease identification standard.

[0038] Since the portable potato detached leaf inoculation fungal disease resistance identification device of the present application can be used by charging or plugging in, is convenient to carry, and the leaves can be tested or preserved in time after collection, which ensures the activity of the leaves, improves the accuracy of the experiment, and overcomes the geographical limitations of the experiment. Moreover, the portable potato detached leaf inoculation fungal disease resistance identification device provided by the present application can also adapt to inoculation experiments of different scales, avoiding waste of resources and environmental pollution.

[0039] For those skilled in the art, it is obvious that the present application is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present application, the present application can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present application is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present application. Any reference signs in the claims should not be construed as limiting the claims involved.

[0040] In addition, it should be understood that although this specification is described in terms of embodiments, not every embodiment only contains an independent technical solution. This narrative manner of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A portable device for identifying resistance to fungal diseases by inoculating detached potato leaves, characterized in that: include: An outer box, an inner box, an inoculation measurement observation plate, a water absorption layer and a top box, wherein the outer box and the top box are opaque, and the inner box and the inoculation measurement observation plate are transparent; The water-absorbing layer is laid inside the outer box, and the inner box is placed inside the outer box and is located above the water-absorbing layer; the interior of the inner box is divided into multiple rows of squares, each row of squares is connected from top to bottom, and a portion of a square in each row of squares is used for pasting labels, and the rest of the square is used as a water inlet; supporting rods are fixed in the rest of the squares in each row of squares; The inoculation measurement observation plate is placed on the inner box, the inoculation measurement observation plate has grid scale lines corresponding to each square, and a small hole corresponding to each square is opened on the inoculation measurement observation plate; The top box is covered on the outer box, and the inner top surface of the top box is provided with a temperature control device, a light source and an ultraviolet sterilization lamp.

2. The portable device for identifying resistance to fungal diseases of potato detached leaves by inoculation according to claim 1, characterized in that: The side of the outer box is provided with two opposite handles.

3. The portable potato detached leaf inoculation fungal disease resistance identification device according to claim 2, characterized in that: The side surfaces of the outer box are provided with opposite outer box buckles, and the outer box buckles clamp the top box to the upper part of the outer box.

4. The portable device for identifying resistance to fungal diseases of potato detached leaves by inoculation according to any one of claims 1 to 3, characterized in that: The square in each row of squares used for sticking labels and serving as a water inlet is the leftmost square.

5. The portable device for identifying resistance to fungal diseases of potato detached leaves inoculated according to any one of claims 1 to 3, characterized in that: Each square has three support rods, and the three support rods are evenly arranged in the same plane and located near the bottom of the square.

6. The portable device for identifying resistance to fungal diseases by inoculating potato detached leaves according to claim 5, characterized in that: A fixing clip is mounted on a support rod.

7. The portable device for identifying resistance to fungal diseases of potato detached leaves by inoculation according to any one of claims 1 to 3, characterized in that: The inoculation measurement viewing plate also has a digital scale.

8. The portable device for identifying resistance to fungal diseases of potato detached leaves by inoculation according to any one of claims 1 to 3, characterized in that: The inner top surface of the top box is evenly distributed with three LED light sources; the inner top surface of the top box is provided with two temperature control devices, wherein each temperature control device is distributed between two LED light sources; the inner top surface of the top box is provided with an ultraviolet sterilization lamp, and the ultraviolet sterilization lamp is located between a temperature control device and the middle LED light source.

9. The portable device for identifying resistance to fungal diseases by inoculating detached potato leaves according to claim 8, characterized in that: The outer surface of the top box has a display screen for displaying the temperature, humidity, lighting time, and ultraviolet light irradiation time inside the top box; the outer surface of the top box has a control panel for controlling the opening and disconnection of the temperature control device, the LED light source, and the ultraviolet sterilization lamp.

10. The portable device for identifying resistance to fungal diseases by inoculating detached potato leaves according to claim 8, characterized in that: The outer surface of the top box is provided with a charging or plugging port; the charging or plugging port is connected to a battery, and the battery is connected to a temperature control device, a light source, an ultraviolet sterilization lamp, a display screen and a control panel.

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