Cell culture device for environmental toxicology research

By setting up activated carbon plates in the cell culture device to treat harmful gases and using dual fixed components to stabilize the plates, the problems of gas treatment and placing plates in cell culture are solved, and environmental pollution reduction and sample stability guarantee are achieved.

CN223002943UActive Publication Date: 2025-06-20INSTITUTE OF MICROBIOLOGY JIANGXI ACADEMY OF SCIENCES (JIANGXI INSTITUTE OF WATERSHED ECOLOGY)
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Patent Information

Application Number
CN202422027995.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-08-21
Publication Date
2025-06-20
Estimated Expiration
2034-08-21

AI Technical Summary

Technical Problem

The harmful gases produced during cell culture are directly discharged without treatment, resulting in environmental pollution; at the same time, the installation stability of the plates is poor, which can easily lead to sample leakage.

Method used

An environmental toxicology research cell culture device was designed, including an incubator, a processing chamber and a fixing assembly. There are multiple activated carbon plates in the treatment box for adsorbing and filtering harmful gases; the fixing assembly is double-fixed with telescopic springs and plug-in slots to ensure the stable installation of the placement plate.

Benefits of technology

The adsorption and filtration of activated carbon plates reduces the pollution to the environment; at the same time, the design of the fixed components improves the stability of the placing plates and reduces the risk of sample leakage.

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Abstract

The utility model relates to the technical field of cell culture, and discloses an environmental toxicology research cell culture device which comprises a culture box, the culture box is internally divided into a culture chamber and an equipment chamber, electric heating pipes are arranged in interlayers of the inner walls of the culture chamber, and the bottom of the culture chamber is communicated with a treatment box through an exhaust pipe I; an exhaust pipe II is arranged at the bottom of the treatment box, a plurality of activated carbon plates are inserted into the treatment box, a plurality of L-shaped brackets are symmetrically arranged in the culture chamber, a placing plate is arranged on the brackets in a sliding manner, a fixing assembly for fixing the placing plate is arranged on the brackets, the fixing assembly comprises a fixing plate, a cavity is formed in each bracket, and the fixing plate is arranged in the cavity. One end of the fixing plate is connected into the cavity through a telescopic spring, a limiting groove is formed in the support, the fixing plate is slidably located in the limiting groove, harmful gas is adsorbed and filtered through the activated carbon plate, pollution to the environment is reduced, the stability of the placing plate is guaranteed through the fixing assembly, and a culture sample is prevented from being scattered and leaked.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device for environmental toxicology research. Background Art

[0002] With the rapid development of industrialization, environmental problems have gradually become severe. It is necessary to conduct research on the environment in order to formulate appropriate protection or treatment measures, which involves environmental toxicology research. Environmental toxicology research is a scientific field that studies the effects of harmful substances existing in the environment on organisms and their mechanisms. It is crucial for environmental protection, public health, and ecosystem management, and helps us understand and address various environmental challenges faced by modern society.

[0003] When conducting research, the influence of the environment on cells is usually observed by culturing cells. However, some harmful gases are generated during the cell culture process. If directly discharged, it will pollute the environment. At the same time, the placement plate in the incubator is usually set to be slidable, without a fixing component, resulting in poor stability and easy sample leakage. Content of the Utility Model

[0004] (1). Technical Problems to be Solved

[0005] The technical problem to be solved by the utility model is to overcome the defects that the above-mentioned gases are directly discharged without treatment and the installation stability of the placement plate is poor, and to provide a cell culture device for environmental toxicology research.

[0006] (2). Technical Solutions

[0007] To solve the above technical problems, the technical solution provided by the utility model is: a cell culture device for environmental toxicology research, including an incubator. The incubator is divided into a culture chamber and an equipment chamber. Electric heating tubes are arranged in the inner wall interlayer of the culture chamber. The bottom of the culture chamber is connected with a treatment box through an exhaust pipe 1. An exhaust pipe 2 is arranged at the bottom of the treatment box. A plurality of activated carbon plates are inserted in the treatment box. A plurality of L-shaped brackets are symmetrically arranged in the culture chamber. A placement plate is slidably arranged on the brackets. A fixing component for fixing the placement plate is arranged on the brackets. The fixing component includes a fixing plate. A cavity is arranged in the bracket. One end of the fixing plate is connected and arranged in the cavity through a telescopic spring. A limiting groove is arranged on the bracket. The fixing plate is slidably located in the limiting groove.

[0008] As an improvement: An air tank is placed in the equipment chamber. An air pump is arranged at the top of the incubator. An air pipe 1 and an air pipe 2 are respectively connected to the air pump. The air pipe 1 is connected to the air tank, and the air pipe 2 is communicated with the culture chamber. The air pump introduces the culture gas in the air tank into the culture chamber.

[0009] As an improvement: a temperature sensor is connected to the top inside the culture chamber, and a control box is provided on the top of the incubator. The temperature sensor and the electric heating tube are respectively electrically connected to the control box. The temperature sensor senses the temperature inside the culture chamber and is controlled by the control box to ensure a constant temperature inside the culture chamber.

[0010] As an improvement: insertion rods are provided on the fixed plate, and slots adapted to the insertion rods are provided on both sides of the placement plate. The insertion rods are inserted into the slots to fix the placement plate and prevent the placement plate from slipping.

[0011] As an improvement: the number of the placement plates is three, and a plurality of ventilation holes are provided on the surface of the placement plate. The arrangement of the ventilation holes ensures the circulation of gas and enables the same gas concentration on each layer of the placement plate.

[0012] As an improvement: a box door is hinged on the outer wall of the incubator, and a transparent glass window is provided on the box door. The glass window on the box door facilitates observing the internal cell culture state.

[0013] (III) Beneficial effects

[0014] The advantages of the present utility model compared with the prior art are as follows:

[0015] 1. By arranging a plurality of activated carbon plates in the treatment box, harmful substances contained in the discharged gas are adsorbed and filtered, thereby reducing environmental pollution. At the same time, the activated carbon plates are convenient to disassemble and clean to ensure the treatment effect;

[0016] 2. The placement plate is clamped by the fixed plate through the action of the telescopic spring, and at the same time, the insertion rod is inserted into the slot, playing a dual fixing role, improving the stability of the placement plate and reducing the probability of the placement plate slipping and the sample spilling. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic structural diagram of a cell culture device for environmental toxicology research of the present utility model.

[0018] Figure 2 is a partially enlarged schematic structural diagram of part A of a cell culture device for environmental toxicology research of the present utility model.

[0019] Figure 3 is a right-view schematic structural diagram of a cell culture device for environmental toxicology research of the present utility model.

[0020] Figure 4 is a sectional structural diagram taken along line A-A of a cell culture device for environmental toxicology research of the present utility model.

[0021] Figure 5 is a partially enlarged schematic structural diagram of part B of a cell culture device for environmental toxicology research of the present utility model.

[0022] Figure 6 It is a schematic structural diagram of a placement plate of a cell culture device for environmental toxicology research of the present utility model.

[0023] As shown in the figure: 1. Incubator; 2. Culture chamber; 3. Equipment room; 4. Electric heating tube; 5. Exhaust pipe 1; 6. Treatment box; 7. Exhaust pipe 2; 8. Activated carbon plate; 9. Bracket; 10. Placement plate; 11. Fixed plate; 12. Telescopic spring; 13. Limit groove; 14. Gas tank; 15. Air pump; 16. Vent pipe 1; 17. Vent pipe 2; 18. Temperature sensor; 19. Control box; 20. Plug rod; 21. Slot; 22. Vent hole; 23. Chamber door. Specific embodiments

[0024] The following further describes the present utility model in detail with reference to the accompanying drawings. The same components are denoted by the same reference numerals.

[0025] It should be noted that the terms "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to the directions in the accompanying drawings, and the terms "inner" and "outer" respectively refer to the directions towards or away from the geometric center of a specific component.

[0026] In order to make the content of the present utility model easier to be clearly understood, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

[0027] Embodiment 1

[0028] Combined with the attached Figures 1 to 5 As shown, an environmental toxicology research cell culture device includes an incubator 1. The incubator 1 is divided into a culture chamber 2 and an equipment room 3. Electric heating tubes 4 are provided in the inner wall interlayer of the culture chamber 2. The bottom of the culture chamber 2 is communicated with a treatment box 6 through an exhaust pipe 1. An exhaust pipe 2 is provided at the bottom of the treatment box 6. A plurality of activated carbon plates 8 are inserted in the treatment box 6. A plurality of L-shaped brackets 9 are symmetrically provided in the culture chamber 2. A placement plate 10 is slidably provided on the bracket 9. A fixing component for fixing the placement plate 10 is provided on the bracket 9. The fixing component includes a fixed plate 11. A cavity is provided in the bracket 9. One end of the fixed plate 11 is connected to be arranged in the cavity through a telescopic spring 12. A limit groove 13 is provided on the bracket 9. The fixed plate 11 is slidably located in the limit groove 13.

[0029] Through the above structure, the discharged gas passes through a plurality of activated carbon plates 8. Activated carbon has a large specific surface area, which can adsorb harmful gases in the discharged gas and remove particulate matter in the gas, reducing environmental pollution caused by direct discharge. The placement plate 10 is firmly connected to the bracket 9 through the fixing component, reducing the probability of the placement plate 10 slipping and ensuring the stability of sample placement.

[0030] Combined with the attached Figure 4 As shown, an air tank 14 is placed in the equipment room 3. An air pump 15 is provided at the top of the incubator 1. An air pipe 16 and an air pipe 17 are respectively connected to the air pump 15. The air pipe 16 is connected to the air tank 14, and the air pipe 17 is connected to the culture chamber 2. A temperature sensor 18 is connected to the top of the culture chamber 2. A control box 19 is provided at the top of the incubator 1. The temperature sensor 18 and the electric heating tube 4 are respectively electrically connected to the control box 19.

[0031] With the above structure, the air pump 15 introduces the culture gas in the air tank 14 into the culture chamber 2. The temperature sensor 18 senses the temperature in the culture chamber 2 and is controlled by the control box 19 to ensure a constant temperature in the culture chamber 2.

[0032] Example Two

[0033] On the basis of Example One, combined with the attached Figure 1 、 Figure 5 and Figure 6 As shown, a plug rod 20 is provided on the fixed plate 11. Slots 21 adapted to the plug rod 20 are provided on both sides of the placement plate 10. The number of placement plates 10 is three. A plurality of ventilation holes 22 are provided on the surface of the placement plate 10. A box door 23 is hinged on the outer wall of the culture chamber 2. A transparent glass window is provided on the box door 23.

[0034] With the above structure, the plug rod 20 is inserted into the slot 21 to fix the placement plate 10, preventing the placement plate 10 from slipping. The arrangement of the ventilation holes 22 ensures the circulation of gas, enabling the same gas concentration on each layer of the placement plate 10. The glass window on the box door 23 facilitates observing the internal cell culture state.

[0035] The specific usage method is as follows:

[0036] The placement plate 10 is slidably installed on the bracket 9. The fixed plate 11 is lifted upward so that the plug rod 20 is aligned with the slot 21 and inserted. Under the action of the telescopic spring 12, the fixed plate 11 clamps the placement plate 10, enabling the fixed plate 11 to be stably placed. The culture dish is placed on the placement plate 10;

[0037] The air pump 15 pumps the gas in the air tank 14 into the culture chamber 2. At the same time, the electric heating tube 4 heats up. The temperature sensor 18 detects the temperature in the culture chamber 2 and transmits the signal to the control box 19 for processing by the control box 19. After reaching the set temperature, the control box 19 controls the electric heating tube 4 to stop heating. If the temperature is lower than the set value, the control box 19 controls the electric heating tube 4 to start heating again. The gas enters the treatment box 6 through the discharge pipe 1, and after being adsorbed and filtered by multiple activated carbon plates 8, it is finally discharged into the air through the discharge pipe 2.

[0038] The above describes the present utility model and its implementation manners. Such description is not restrictive. What is shown in the drawings is only one of the implementation manners of the present utility model, and the actual structure is not limited thereto. In general, if those of ordinary skill in the art are inspired by it and without departing from the gist of the creation of the present utility model, they design without creativity structural manners and embodiments similar to the technical solution, which shall fall within the protection scope of the present utility model.

Claims

1. A cell culture device for environmental toxicology research, comprising an incubator (1), characterized in that: The culture box (1) is divided into a culture chamber (2) and an equipment chamber (3), the inner wall interlayer of the culture chamber (2) is provided with an electric heating pipe (4), the bottom of the culture chamber (2) is connected to a treatment box (6) through an exhaust pipe 1 (5), the bottom of the treatment box (6) is provided with an exhaust pipe 2 (7), and a plurality of activated carbon plates (8) are inserted in the treatment box (6); A plurality of L-shaped brackets (9) are symmetrically arranged in the culture chamber (2), a placement plate (10) is slidably arranged on the bracket (9), a fixing assembly for fixing the placement plate (10) is arranged on the bracket (9), and the fixing assembly comprises a fixing plate (11), a cavity is arranged in the bracket (9), one end of the fixing plate (11) is connected to the cavity via a telescopic spring (12), a limiting groove (13) is arranged on the bracket (9), and the fixing plate (11) is slidably located in the limiting groove (13).

2. The cell culture device for environmental toxicology research according to claim 1, characterized in that: A gas tank (14) is placed in the equipment room (3), an air pump (15) is provided on the top of the culture box (1), and a ventilation pipe 1 (16) and a ventilation pipe 2 (17) are respectively connected to the air pump (15), the ventilation pipe 1 (16) is connected to the gas tank (14), and the ventilation pipe 2 (17) is connected to the culture room (2).

3. The cell culture device for environmental toxicology research according to claim 1, characterized in that: A temperature sensor (18) is connected to the top of the culture chamber (2), a control box (19) is provided on the top of the culture box (1), and the temperature sensor (18) and the electric heating tube (4) are electrically connected to the control box (19) respectively.

4. The cell culture device for environmental toxicology research according to claim 1, characterized in that: The fixing plate (11) is provided with an insertion rod (20), and both sides of the placement plate (10) are provided with slots (21) adapted to the insertion rod (20).

5. The cell culture device for environmental toxicology research according to claim 1, characterized in that: The number of the placement plates (10) is three, and a plurality of ventilation holes (22) are provided on the surface of the placement plates (10).

6. The cell culture device for environmental toxicology research according to claim 1, characterized in that: A door (23) is hingedly provided on the outer wall of the culture chamber (2), and a transparent glass window is provided on the door (23).