Raw material gradient dilution device for enzyme-linked immunosorbent assay

Abstract

The raw material gradient dilution device comprises a dilution tank and a stirring assembly, a bearing plate is fixedly installed on one side of the top face of the dilution tank, the stirring assembly is installed on the bearing plate through a lead screw module, a feeding port is formed in the other side of the top face of the dilution tank, and a feeding port is formed in the other side of the top face of the dilution tank. A cleaning pipe is hoisted on the inner top surface of the dilution tank, cleaning nozzles are obliquely arranged on the surface of the cleaning pipe, and a booster pump is fixedly mounted on the surface of one side of the dilution tank. According to the device, the stirring assembly is arranged, the outer cylinder and the inner cylinder rotate in opposite directions, a detection sample in the dilution box can be stirred in multiple directions, and meanwhile, the lead screw module can drive the stirring assembly to reciprocate up and down in the stirring process of the detection sample, so that the stirring range of the detection sample can be expanded; the stirring effect and efficiency of the detection sample are improved, the time required for uniformly stirring the detection sample is shortened, and the dilution effect of the detection sample is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental equipment, in particular to a raw material gradient dilution device for enzyme-linked immunosorbent assay (ELISA). Background Technique

[0002] When analyzing and testing samples with existing sample analysis instruments, the requirements for the samples to be tested are different according to different test items. For example, some test items require diluting high-concentration samples first to complete the test with the diluted low-concentration samples. At the same time, after the high-concentration sample solution and the diluent are jointly injected, corresponding dilution and mixing are required to facilitate the test analysis by the analysis instrument.

[0003] The existing technology has the following problems:

[0004] After a large number of searches, it is found that the Chinese patent publication number: CN202322405635.7 discloses a medical sample dilution device. The above patent still has problems in actual use. More obviously, the time required to stir the test sample evenly is relatively long, and the stirring range of the test sample is limited, which affects the stirring efficiency and effect of the test sample.

[0005] Therefore, we propose a raw material gradient dilution device for enzyme-linked immunosorbent assay to solve the above drawbacks. Content of the Utility Model

[0006] The purpose of the utility model is to provide a raw material gradient dilution device for enzyme-linked immunosorbent assay to solve the deficiencies existing in the prior art.

[0007] To achieve the above purpose, the utility model adopts the following technical scheme: A raw material gradient dilution device for enzyme-linked immunosorbent assay, including a dilution tank and a stirring component. One side of the top surface of the dilution tank is fixedly installed with a bearing plate, and the stirring component is installed on the bearing plate through a lead screw module. The other side of the top surface of the dilution tank is provided with a feed inlet. A cleaning pipe is suspended from the inner top surface of the dilution tank, and cleaning nozzles are inclined on the surface of the cleaning pipe. One side surface of the dilution tank is fixedly installed with a booster pump, and the water outlet end of the booster pump is communicated with the cleaning pipe. A liquid storage tank is arranged on one side of the dilution tank. The water inlet end of the booster pump is installed with a water inlet pipe, and one end of the water inlet end extends into the interior of the liquid storage tank. The bottom surface of the dilution tank is fixedly installed with a discharge port;

[0008] The stirring assembly includes a carrier frame, a driving motor, a first bevel gear, an outer cylinder, an inner cylinder, a second bevel gear, a third bevel gear, a support cylinder, a chamber, a connecting rod and a scraper. The inner cylinder penetrates through the interior of the outer cylinder. Support cylinders are fixedly connected to the surfaces of both the outer cylinder and the inner cylinder, and a chamber is provided at one end of the support cylinder. A connecting rod is slidably connected inside the chamber, and a scraper is installed at the other end of the connecting rod. The surface of the scraper is in close contact with the inner wall of the dilution tank. One ends of the outer cylinder and the inner cylinder are rotatably connected to the carrier frame, and a driving motor is fixedly installed on the surface of the carrier frame. A first bevel gear is installed at the output end of the driving motor. Second bevel gears and third bevel gears are respectively installed on the surfaces of the outer cylinder and the inner cylinder. The first bevel gear meshes with the second bevel gear and the third bevel gear.

[0009] Preferably, a control panel is fixedly installed on the outer wall of the dilution tank, and the control panel is electrically connected to the driving motor, the lead screw module and the booster pump through wires.

[0010] Preferably, there are multiple scrapers, and the multiple scrapers are distributed in a circular array inside the dilution tank.

[0011] Preferably, the angle between the cleaning spray head and the inner wall of the dilution tank is 15°-55°.

[0012] Preferably, there are multiple cleaning spray heads, and the multiple cleaning spray heads are distributed in a circular array on the surface of the cleaning pipe.

[0013] Preferably, the diameter of the cleaning pipe is smaller than the diameter of the dilution tank.

[0014] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0015] 1. In the present utility model, by setting the stirring assembly, the outer cylinder and the inner cylinder rotate in opposite directions, which can stir the test sample inside the dilution tank in multiple directions. At the same time, during the stirring process of the test sample, the lead screw module can drive the stirring assembly to move up and down reciprocally, thereby expanding the stirring range of the test sample, being beneficial to improving the stirring effect and efficiency of the test sample, reducing the time required for the test sample to be stirred evenly, and ensuring the dilution effect of the test sample.

[0016] 2. In the present utility model, the cleaning agent inside the liquid storage tank is pumped into the cleaning pipe by the booster pump and then sprayed out from several cleaning spray heads annularly arranged on the surface of the cleaning pipe, which can be sprayed on the inner wall of the dilution tank. On the one hand, it is convenient to rinse the inner wall of the dilution tank after use. On the other hand, it will not cause the problem of residual test sample on the inner wall of the dilution tank. On the third hand, it is convenient for the next use. Description of the Drawings

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0018] Figure 1 Structural schematic diagram of the raw material gradient dilution device for enzyme-linked immunosorbent assay proposed by the present invention;

[0019] Figure 2 Structural schematic diagram of the stirring assembly;

[0020] Figure 3 External structural schematic diagram of the raw material gradient dilution device for enzyme-linked immunosorbent assay proposed by the present invention;

[0021] Figure 4 Bottom view of the cleaning pipe and the cleaning nozzle.

[0022] Legend:

[0023] 1. Dilution tank; 2. Feed inlet; 3. Discharge port; 4. Carrier plate; 5. Lead screw module; 6. Stirring assembly; 61. Carrier frame; 62. Driving motor; 63. First bevel gear; 64. Outer cylinder; 65. Inner cylinder; 66. Second bevel gear; 67. Third bevel gear; 68. Support cylinder; 69. Chamber; 610. Connecting rod; 611. Scraper; 7. Liquid storage tank; 8. Booster pump; 9. Water inlet pipe; 10. Cleaning pipe; 11. Cleaning nozzle; 12. Control panel. Detailed implementation manners

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0025] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation to the present utility model; the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance; in addition, unless otherwise clearly specified and defined, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0026] Please refer to Figures 1-4 , the raw material gradient dilution device for enzyme-linked immunosorbent assay, includes a dilution tank 1 and a stirring assembly 6. One side of the top surface of the dilution tank 1 is fixedly installed with a bearing plate 4, and a stirring assembly 6 is installed on the bearing plate 4 through a lead screw module 5. The other side of the top surface of the dilution tank 1 is provided with a feed port 2. A cleaning pipe 10 is suspended from the inner top surface of the dilution tank 1, and cleaning nozzles 11 are inclined on the surface of the cleaning pipe 10. One side surface of the dilution tank 1 is fixedly installed with a booster pump 8, and the water outlet end of the booster pump 8 is communicated with the cleaning pipe 10. A liquid storage tank 7 is arranged on one side of the dilution tank 1. The water inlet end of the booster pump 8 is installed with a water inlet pipe 9, and one end of the water inlet end extends into the interior of the liquid storage tank 7. The bottom surface of the dilution tank 1 is fixedly installed with a discharge port 3;

[0027] The stirring assembly 6 includes a bearing frame 61, a driving motor 62, a first bevel gear 63, an outer cylinder 64, an inner cylinder 65, a second bevel gear 66, a third bevel gear 67, a support cylinder 68, a chamber 69, a connecting rod 610 and a scraper 611. The inner cylinder 65 penetrates through the interior of the outer cylinder 64. Support cylinders 68 are fixedly connected to the surfaces of the outer cylinder 64 and the inner cylinder 65, and a chamber 69 is opened at one end of the support cylinder 68. A connecting rod 610 is slidably connected to the interior of the chamber 69, and a scraper 611 is installed at the other end of the connecting rod 610, and the surface of the scraper 611 is in close contact with the inner wall of the dilution tank 1. One ends of the outer cylinder 64 and the inner cylinder 65 are rotatably connected to the bearing frame 61, and a driving motor 62 is fixedly installed on the surface of the bearing frame 61, and a first bevel gear 63 is installed at the output end of the driving motor 62. Second bevel gears 66 and third bevel gears 67 are respectively installed on the surfaces of the outer cylinder 64 and the inner cylinder 65, and the first bevel gear 63 meshes with the second bevel gear 66 and the third bevel gear 67.

[0028] During use, the drive motor 62 drives the first bevel gear 63 to rotate. The rotation of the first bevel gear 63 drives the second bevel gear 66 and the third bevel gear 67 to rotate in the opposite direction, so that the outer cylinder 64 and the inner cylinder 65 rotate in the opposite direction, enabling multi-directional stirring of the test sample inside the dilution tank 1. At the same time, during the stirring process of the test sample, the lead screw module 5 can drive the stirring assembly 6 to move up and down reciprocally, thereby expanding the stirring range of the test sample, facilitating improving the stirring effect and efficiency of the test sample, reducing the time required for uniformly stirring the test sample. The cleaning agent in the liquid storage tank 7 is pumped into the cleaning pipe 10 through the booster pump 8 and then sprayed out from a number of cleaning nozzles 11 annularly arranged on the surface of the cleaning pipe 10, which can be sprayed on the inner wall of the dilution tank 1. On the one hand, it is convenient to rinse the inner wall of the dilution tank 1 after use. On the other hand, it will not cause the problem of residual test sample on the inner wall of the dilution tank 1. On the third hand, it is convenient for the next use.

[0029] In this embodiment: A control panel 12 is fixedly installed on the outer wall of the dilution tank 1, and the control panel 12 is electrically connected to the drive motor 62, the lead screw module 5, and the booster pump 8 through wires.

[0030] Specifically, it is a common circuit connection structure and will not be elaborated here.

[0031] In this embodiment: There are multiple scraping plates 611, and the multiple scraping plates 611 are annularly and arrayedly distributed inside the dilution tank 1.

[0032] Specifically, it improves the cleaning effect and efficiency of the test sample on the inner wall of the dilution tank 1.

[0033] In this embodiment: The included angle between the cleaning nozzle 11 and the inner wall of the dilution tank 1 is 15° - 55°.

[0034] Specifically, the included angle between the cleaning nozzle 11 and the inner wall of the dilution tank 1 can be flexibly selected and adjusted as needed to ensure the cleaning effect and efficiency of the inner wall of the dilution tank 1.

[0035] In this embodiment: There are multiple cleaning nozzles 11, and the multiple cleaning nozzles 11 are annularly and arrayedly distributed on the surface of the cleaning pipe 10.

[0036] Specifically, it further improves the cleaning effect and efficiency of the inner wall of the dilution tank 1.

[0037] In this embodiment: The diameter of the cleaning pipe 10 is smaller than the diameter of the dilution tank 1.

[0038] Specifically, the cleaning pipe 10 can be installed inside the dilution tank 1.

[0039] In this embodiment: The control panel 12 is of an existing structure, and the control circuit can be realized by simple programming by those skilled in the art, which belongs to the common general knowledge in the art. Only its use is involved without modification, so the control method and circuit connection will not be described in detail.

[0040] Working principle: During use, the driving motor 62 drives the first bevel gear 63 to rotate. The rotation of the first bevel gear 63 drives the second bevel gear 66 and the third bevel gear 67 to rotate in the opposite direction, so that the outer cylinder 64 and the inner cylinder 65 rotate in the opposite direction, which can stir the test sample inside the dilution tank 1 in multiple directions. At the same time, during the stirring process of the test sample, the lead screw module 5 can drive the stirring assembly 6 to move up and down reciprocally, so as to expand the stirring range of the test sample, which is beneficial to improving the stirring effect and efficiency of the test sample and reducing the time required for the test sample to be stirred evenly. The cleaning agent in the liquid storage tank 7 is pumped into the cleaning pipe 10 by the booster pump 8 and then sprayed out from several cleaning nozzles 11 annularly arranged on the surface of the cleaning pipe 10, which can be sprayed on the inner wall of the dilution tank 1. On the one hand, it is convenient to rinse the inner wall of the dilution tank 1 after use. On the other hand, it will not cause the problem of residual test sample on the inner wall of the dilution tank 1. On the third hand, it is convenient for the next use.

[0041] It should be noted that: The model specifications of the driving motor 62, the lead screw module 5 and the booster pump 8 need to be selected and determined according to the actual specifications of the device, etc. The specific selection calculation method adopts the existing technology in the art, so it will not be elaborated in detail.

[0042] The power supply and its principle of the driving motor 62, the lead screw module 5 and the booster pump 8 are clear to those skilled in the art and will not be described in detail here.

[0043] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A raw material gradient dilution device for enzyme-linked immunosorbent assay, comprising a dilution box (1) and a stirring assembly (6), characterized in that: A bearing plate (4) is fixedly mounted on one side of the top surface of the dilution box (1), and a stirring assembly (6) is mounted on the bearing plate (4) via a screw module (5); a feed port (2) is mounted on the other side of the top surface of the dilution box (1); a cleaning pipe (10) is suspended on the inner top surface of the dilution box (1), and a cleaning nozzle (11) is obliquely arranged on the surface of the cleaning pipe (10); a booster pump (8) is fixedly mounted on one side of the surface of the dilution box (1), and a water outlet of the booster pump (8) is connected to the cleaning pipe (10); a liquid storage tank (7) is arranged on one side of the dilution box (1); a water inlet pipe (9) is mounted on the water inlet end of the booster pump (8), and one end of the water inlet end extends to the interior of the liquid storage tank (7); and a discharge port (3) is fixedly mounted on the bottom surface of the dilution box (1); The stirring assembly (6) comprises a carrier frame (61), a driving motor (62), a first bevel gear (63), an outer cylinder (64), an inner cylinder (65), a second bevel gear (66), a third bevel gear (67), a support cylinder (68), a chamber (69), a connecting rod (610) and a scraper (611), wherein the inner cylinder (65) is provided through the interior of the outer cylinder (64), the surfaces of the outer cylinder (64) and the inner cylinder (65) are fixedly connected with the support cylinder (68), and a chamber (69) is provided at one end of the support cylinder (68), and the interior of the chamber (69) is slidably connected with the connecting rod (610), and the connecting rod A scraper (611) is installed at the other end of the outer cylinder (610), and the surface of the scraper (611) is tightly fitted with the inner wall of the dilution box (1). One end of the outer cylinder (64) and the inner cylinder (65) are rotatably connected to the support frame (61), and a driving motor (62) is fixedly installed on the surface of the support frame (61), and a first bevel gear (63) is installed at the output end of the driving motor (62). A second bevel gear (66) and a third bevel gear (67) are installed on the surfaces of the outer cylinder (64) and the inner cylinder (65), respectively, and the first bevel gear (63) is meshed with the second bevel gear (66) and the third bevel gear (67).

2. The raw material gradient dilution device for ELISA according to claim 1, characterized in that: A control panel (12) is fixedly mounted on the outer wall of the dilution box (1), and the control panel (12) is electrically connected to the drive motor (62), the lead screw module (5) and the booster pump (8) via wires.

3. The raw material gradient dilution device for ELISA according to claim 1, characterized in that: A plurality of scrapers (611) are provided, and the plurality of scrapers (611) are distributed in a circular array inside the dilution box (1).

4. The raw material gradient dilution device for ELISA according to claim 1, characterized in that: The angle between the cleaning nozzle (11) and the inner wall of the dilution box (1) is 15°-55°.

5. The raw material gradient dilution device for ELISA according to claim 1, characterized in that: A plurality of cleaning nozzles (11) are provided, and the plurality of cleaning nozzles (11) are distributed in a circular array on the surface of the cleaning pipe (10).

6. The raw material gradient dilution device for ELISA according to claim 1, characterized in that: The diameter of the cleaning pipe (10) is smaller than the diameter of the dilution tank (1).

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