Device for sufficient oxygenation of perfusate and oxygen

Abstract

The utility model discloses a device for sufficient oxygenation of perfusate and oxygen, which comprises a container body, a cover with an oxygenating port is detachably mounted at the top of the container body, a liquid inlet and a liquid outlet are further formed in the cover, and a pipeline can be connected through the liquid inlet and the liquid outlet to maintain the height of the perfusate in the container body. The tail end of the pipeline is arranged above the liquid level, so that the pipeline does not need to go deep below the liquid level; a groove is formed in the outer wall of the rotating wheel, the rotating wheel is detachably installed in the container body, the rotating wheel is transversely arranged, a machine shaft is installed along the center of the rotating wheel in a penetrating mode, the two ends of the machine shaft are detachably fixed to the container body, and a part of the machine shaft penetrates through the container body; when the rotating wheel is rotated, the perfusate can enter the groove and finally fall into the perfusate, so that the surface area of the perfusate is increased, sufficient exchange of oxygen and the perfusate is achieved, and the effect of sufficient mixing of the oxygen and the perfusate is achieved.

Description

Technical Field

[0001] The utility model relates to the field of experimental devices, and particularly to a device for fully oxygenating perfusion fluid and oxygen. Background Art

[0002] Ex vivo organ perfusion experiment is a commonly used experimental method, often used in research on aspects such as drug metabolism, toxicity, and efficacy. In such experiments, organs such as those of rodents (such as rats and mice) are usually isolated and the blood circulation in the body is simulated through a perfusion system, enabling the perfusion fluid to flow through the organ tissue, thereby simulating the physiological environment in the body and studying the metabolism of drugs in the organ and the corresponding biological effects.

[0003] In ex vivo organ perfusion experiments, oxygen plays an important role in maintaining oxygen supply to organ cells, promoting drug metabolism, preventing cell damage caused by hypoxia, and maintaining acid-base balance. During the perfusion process, in some cases, an appropriate amount of bovine serum albumin needs to be added to the perfusion fluid to increase the osmotic pressure of the perfusion fluid and make it closer to the physiological level. However, continuous oxygenation can cause foaming problems in the perfusion fluid during the perfusion process, resulting in the inability to meet the experimental requirements. Summary of the Utility Model

[0004] The technical problem to be solved by the utility model is that in the existing ex vivo organ perfusion process, in some cases, an appropriate amount of bovine serum albumin needs to be added to the perfusion fluid to increase the osmotic pressure of the perfusion fluid and make it closer to the physiological level. However, continuous oxygenation can cause foaming problems in the perfusion fluid during the perfusion process, resulting in the inability to meet the experimental requirements. The utility model provides a device for fully oxygenating perfusion fluid and oxygen. A container body with a runner is used to oxygenate the perfusion fluid. Grooves are provided on the runner, and when rotating, the runner can lift part of the perfusion fluid and fully oxygenate it with oxygen, effectively solving the problem of foaming of the perfusion fluid caused by oxygenation while achieving the purpose of fully mixing oxygen and perfusion fluid, greatly improving the accuracy of the experiment and the reliability of experimental data, and thus setting up a structure that can replace imported experimental consumables with the same efficacy that need to be purchased, reducing the experimental cost, being simple in structure and convenient to use, so as to solve the defects caused by the existing technology.

[0005] To solve the above technical problems, the utility model provides the following technical solutions:

[0006] A device for fully oxygenating perfusion fluid and oxygen, which includes a container body. A lid with an oxygen inlet is detachably installed on the top of the container body. The oxygen inlet is used to connect to an oxygen pipeline, and the end of the oxygen pipeline is placed above the liquid level of the perfusion fluid in the container body and does not need to extend below the liquid level.

[0007] A runner with grooves on its outer wall, and the runner is detachably installed inside the container body.

[0008] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, the runner is horizontally arranged and a machine shaft is installed through its central part. Both ends of the machine shaft are detachably fixed on the container body and part of the machine shaft penetrates through the container body.

[0009] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, a motor is installed at any one end of the machine shaft. After the motor is started, it drives the machine shaft to rotate. While the machine shaft rotates, it drives the runner to rotate.

[0010] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, the lid is a glass lid, which is convenient for observation.

[0011] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, a liquid inlet and a liquid outlet are also opened on the lid. Through the liquid inlet and the liquid outlet, pipelines can be connected to maintain the height of the perfusion fluid in the container body.

[0012] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, the liquid inlet and the liquid outlet are arranged on both sides of the oxygen filling port.

[0013] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, the outer wall of the runner is provided with a plurality of grooves. When the runner rotates, part of the perfusion fluid in the container body will enter the grooves of the runner, driving the perfusion fluid up, increasing the surface area of the perfusion fluid, facilitating the full oxygenation of the perfusion fluid and oxygen, and reducing the problem of perfusion fluid foaming.

[0014] In the above-mentioned device for fully oxygenating perfusion fluid and oxygen, the outer wall of the runner is provided with a plurality of irregular grooves. When the runner rotates, part of the perfusion fluid in the container body will enter the grooves of the runner, driving the perfusion fluid up, increasing the surface area of the perfusion fluid, facilitating the full oxygenation of the perfusion fluid and oxygen, and reducing the problem of perfusion fluid foaming. The irregular grooves can maximize oxygenation.

[0015] During the oxygen filling process, part of the runner is below the liquid level of the perfusion fluid and part is above the liquid level of the perfusion fluid. The motor drives the runner to rotate self - clockwise (continuously rotating out of the perfusion fluid and then rotating into the perfusion fluid), increasing the surface area of contact between the perfusion fluid and the oxygen in the container body, enabling the perfusion fluid to come into full contact with oxygen and other gases, imitating the gas - blood exchange of an animal's lungs to achieve the oxygenation effect.

[0016] The above-mentioned device for fully oxygenating perfusion fluid and oxygen, wherein a partition integrally formed with the container body is installed inside the container body, and the partition and the container body form an interlayer chamber. An inlet pipe and an outlet pipe respectively communicating with the interlayer chamber are installed on the container body. The outlet pipe is higher than the inlet pipe in height. After the inlet pipe and the outlet pipe are communicated with a circulating water path, a water bath circulation is carried out on the container body to achieve a constant temperature state, so as to maintain the temperature of the perfusion fluid in the container body.

[0017] The above-mentioned device for fully oxygenating perfusion fluid and oxygen, wherein the inlet pipe and the outlet pipe are respectively arranged on both sides of the container body.

[0018] According to the technical solution provided by the above-mentioned device for fully oxygenating perfusion fluid and oxygen of the present invention, the following technical effects are achieved:

[0019] The container body with a runner is used to oxygenate the perfusion fluid. The runner is provided with grooves, which can lift part of the perfusion fluid while rotating and fully oxygenate it with oxygen. In the in vitro organ perfusion model, while achieving the purpose of fully mixing oxygen and perfusion fluid, it can effectively solve the problem of perfusion fluid foaming caused by oxygenation, greatly improving the operability, accuracy of the experiment and the reliability of experimental data. Therefore, it is set to replace imported experimental consumables with the same efficacy that need to be purchased, reducing the experimental cost. The structure is simple and easy to use. Description of the Drawings

[0020] Figure 1 It is a schematic structural diagram of the device for fully oxygenating perfusion fluid and oxygen of the present invention;

[0021] Figure 2 It is a schematic structural diagram of the runner with irregular grooves in the device for fully oxygenating perfusion fluid and oxygen of the present invention;

[0022] Figure 3 It is a front view structural diagram of the device for fully oxygenating perfusion fluid and oxygen of the present invention;

[0023] Figure 4 It is a top view structural diagram of the device for fully oxygenating perfusion fluid and oxygen of the present invention without a lid;

[0024] Figure 5 It is a top view structural diagram of the lid in the device for fully oxygenating perfusion fluid and oxygen of the present invention.

[0025] Among them, the reference numerals are as follows:

[0026] Container body 1, water outlet pipe 2, water inlet pipe 3, machine shaft 4, runner 5, lid 6, liquid inlet 7, liquid outlet 8, oxygenation port 9. Detailed implementation mode

[0027] In order to make the technical means, creative features, achieved purposes and functions realized by the utility model easy to understand, the technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with specific drawings. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all of the embodiments.

[0028] All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts belong to the scope protected by the present utility model.

[0029] It should be noted that the structures, ratios, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those who are familiar with this technology to understand and read, and are not used to limit the limiting conditions under which the present utility model can be implemented. Therefore, they do not have technical substantive significance. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the functions that the present utility model can produce and the purposes that can be achieved, should still fall within the scope that can be covered by the technical content disclosed by the present utility model.

[0030] At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" cited in this specification are only for the convenience of clear narration, rather than used to limit the scope under which the present utility model can be implemented. The change or adjustment of their relative relationships, without substantial change of the technical content, should also be regarded as the scope under which the present utility model can be implemented.

[0031] As Figures 1-5 shown, in the first embodiment, a device for fully oxygenating perfusion fluid and oxygen, which includes a container body 1. A lid 6 with an oxygenation port 9 is detachably installed on the top of the container body 1. The lid 6 is also provided with a liquid inlet 7 and a liquid outlet 8. Through the liquid inlet 7 and the liquid outlet 8, pipelines can be connected to maintain the height of the perfusion fluid in the container body 1. Specifically, the liquid inlet 7 and the liquid outlet 8 can be connected to pipelines, and under the action of an external peristaltic pump, the constant height of the perfusion fluid level can be maintained. The oxygenation port 9 can be connected to a pipeline for oxygenation, and the end of the pipeline is placed above the liquid level and does not need to extend below the liquid level;

[0032] As Figure 2As shown, there is a runner 5 with grooves on its outer wall. The runner 5 is detachably installed inside the container body 1. The runner 5 is horizontally arranged and a machine shaft 4 is installed through its central part. Both ends of the machine shaft 4 are detachably fixed on the container body 1 and part of the machine shaft 4 penetrates through the container body 1. A motor is installed at any one end of the machine shaft 4. After the motor is started, it drives the machine shaft 4 to rotate. While the machine shaft 4 rotates, it drives the runner 5 to rotate. At the same time, the perfusion liquid will enter the grooves and finally fall into the perfusion liquid, increasing the surface area of the perfusion liquid, so as to realize the full exchange of oxygen and the perfusion liquid and achieve the effect of fully mixing oxygen and the perfusion liquid.

[0033] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the lid 6 is a glass lid.

[0034] As Figure 5 As shown, in the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the liquid inlet 7 and the liquid outlet 8 are arranged on both sides of the oxygen filling port 9.

[0035] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the outer wall of the runner 5 is provided with a plurality of irregular grooves. When the runner 5 rotates, part of the perfusion liquid in the container body 1 will enter the grooves of the runner 5, driving the perfusion liquid up and increasing the surface area of the perfusion liquid, facilitating the full oxygenation of the perfusion liquid and oxygen and reducing the problem of the perfusion liquid foaming. The irregular grooves can maximize the oxygenation.

[0036] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, a partition plate integrally formed with the container body 1 is installed inside the container body 1. The partition plate and the container body 1 form an interlayer chamber. The container body 1 is provided with a water inlet pipe 3 and a water outlet pipe 2 respectively communicating with the interlayer chamber. The water outlet pipe 2 is higher than the water inlet pipe 3 in height.

[0037] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the water inlet pipe 3 and the water outlet pipe 2 are respectively arranged on both sides of the container body 1.

[0038] As Figures 1-5 As shown, in the second embodiment, a device for fully oxygenating the perfusion liquid and oxygen includes a container body 1. A lid 6 with an oxygen filling port 9 is detachably installed on the top of the container body 1. The lid 6 is also provided with a liquid inlet 7 and a liquid outlet 8. Through the liquid inlet 7 and the liquid outlet 8, pipelines can be connected to maintain the height of the perfusion liquid in the container body 1. Specifically, the liquid inlet 7 and the liquid outlet 8 can be connected to pipelines, and under the action of an external peristaltic pump, the constant height of the perfusion liquid level can be maintained. The oxygen filling port 9 can be connected to a pipeline for oxygen filling. The end of the pipeline is placed above the liquid level and does not need to go deep below the liquid level.

[0039] The runner 5 with grooves on its outer wall is detachably installed inside the container body 1. The runner 5 is horizontally arranged and a machine shaft 4 is installed through its central part. Both ends of the machine shaft 4 are detachably fixed on the container body 1 and partially penetrate the container body 1. A motor is installed at any one end of the machine shaft 4. After the motor is started, it drives the machine shaft 4 to rotate. While the machine shaft 4 rotates, it drives the runner 5 to rotate. When the runner 5 rotates, the perfusion liquid will enter the grooves and finally fall into the perfusion liquid, increasing the surface area of the perfusion liquid, so as to realize the full exchange of oxygen and the perfusion liquid and achieve the effect of fully mixing oxygen and the perfusion liquid.

[0040] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the lid 6 is a glass lid.

[0041] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the liquid inlet 7 and the liquid outlet 8 are arranged on both sides of the oxygen filling port 9.

[0042] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the outer wall of the runner 5 is provided with a plurality of grooves. When the runner 5 rotates, part of the perfusion liquid in the container body 1 will enter the grooves of the runner 5, driving the perfusion liquid up and increasing the surface area of the perfusion liquid, facilitating the full oxygenation of the perfusion liquid and oxygen and reducing the problem of the perfusion liquid foaming.

[0043] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, a partition plate integrally formed with the container body 1 is installed inside the container body 1. The partition plate and the container body 1 form a sandwich chamber. An inlet pipe 3 and an outlet pipe 2 respectively communicating with the sandwich chamber are installed on the container body 1. The outlet pipe 2 is higher than the inlet pipe 3 in height.

[0044] In the above-mentioned device for fully oxygenating the perfusion liquid and oxygen, the inlet pipe 3 and the outlet pipe 2 are respectively arranged on both sides of the container body 1.

[0045] In summary, for the device for fully oxygenating the perfusion liquid and oxygen of the present utility model, a container body with a runner is used to oxygenate the perfusion liquid. The runner is provided with grooves, which can lift part of the perfusion liquid while rotating and fully oxygenate it with oxygen. While achieving the purpose of fully mixing oxygen and the perfusion liquid, it can effectively solve the problem of the perfusion liquid foaming caused by oxygen filling, greatly improving the accuracy of the experiment and the reliability of the experimental data. And thus, it is set to replace the imported experimental consumables that need to be purchased with the same efficacy, reducing the experimental cost. The structure is simple and convenient to use.

[0046] The specific embodiments of the utility model have been described above. It should be understood that the utility model is not limited to the above specific embodiments, and the devices and structures not described in detail therein should be understood to be implemented in a common manner in the art; those skilled in the art can make various deformations or modifications within the scope of the claims, make several simple deductions, deformations or substitutions, which do not affect the essential content of the utility model.

Claims

1. A device for fully oxygenating a perfusion fluid with oxygen, characterized in that: It comprises a container body, and a cover with an oxygen filling port is detachably mounted on the top of the container body; The outer wall is provided with a rotating wheel with a groove, and the rotating wheel can be detachably installed inside the container body.

2. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 1, characterized in that: The rotating wheel is arranged transversely and a machine shaft is installed through the center of the rotating wheel. Both ends of the machine shaft are detachably fixed on the container body and a part of the machine shaft passes through the container body.

3. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 2, characterized in that: A motor is mounted on either end of the shaft.

4. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 1, characterized in that: The cover is a glass cover.

5. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 1, characterized in that: The cover is also provided with a liquid inlet and a liquid outlet.

6. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 5, characterized in that: The liquid inlet and the liquid outlet are arranged on both sides of the oxygen filling port.

7. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 1, characterized in that: The outer wall of the rotating wheel is provided with a plurality of grooves.

8. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 1, characterized in that: The outer wall of the rotating wheel is provided with a plurality of irregular grooves.

9. A device for fully oxygenating a perfusion fluid with oxygen according to any one of claims 1 to 8, characterized in that: A partition formed integrally with the container body is installed inside the container body, and the partition and the container body form an interlayer chamber. A water inlet pipe and a water outlet pipe respectively connected to the interlayer chamber are installed on the container body, and the water outlet pipe is higher than the water inlet pipe.

10. A device for fully oxygenating a perfusion fluid with oxygen as claimed in claim 9, characterized in that: The water inlet pipe and the water outlet pipe are respectively arranged on two sides of the container body.

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