Simulated rumen fermentation device

Abstract

The utility model provides a rumen fermentation simulation device which comprises an annular water bath, the upper portion of the water bath is provided with an opening, and the lower portion of the water bath is connected with the ground through a supporting frame; the rotating support is installed in the middle of the water bath, a rotating shaft of the rotating support coincides with the center line of the water bath, and a plurality of clamps are arranged on the rotating support; and the fermentation tank is positioned in the water bath. The fermentation tank is simultaneously clamped by the plurality of clamps on the rotating bracket and is immersed into the water bath, a constant-temperature state is kept during an experiment, the magnetic stirring beads are placed in the fermentation tank and can sequentially pass through the bulges at the bottom of the water bath under the rotation of the rotating bracket, and the magnetic stirring beads are close to the magnetic stirrer, so that the magnetic stirring beads are uniformly stirred. The stirring device is driven by the rotating magnetic field to rotate, so that liquid in the fermentation tank is stirred to simulate rumen wriggling while sealing of the fermentation tank is guaranteed, liquid layering is avoided, and experimental accuracy is improved.

Description

Technical Field

[0001] The present application relates to the technical field of livestock research, and particularly relates to a simulated rumen fermentation device. Background Art

[0002] Ruminants can utilize the nutritional components of feed with the help of microorganisms through their rumens with special structures. The degradation degree of feed in the rumen, the synthesis efficiency of microbial protein, and the digestibility of undegraded protein and microbial protein of feed entering the small intestine are important parameters in nutritional value evaluation and feeding standards. For example, the paper "Manufacture and Application of Static Fermentation Device" published in the 10th issue of "Heilongjiang Animal Husbandry and Veterinary Medicine" in 2015 discloses a device that simulates the rumen of ruminants. After opening holes in the cover of the tank body, the gas in the tank is exported through a trachea for analysis, and such experiments have become an extended application of in vitro gas production experiments. In order to simulate the rumen environment, in vitro gas production experiments usually require a water bath, and the tank body needs to be kept sealed. At the same time, multiple control groups are set up during the experiment. Since the device lacks a stirring device during the experiment, during the simulation process, the device needs to be shaken regularly, so that the liquid components in the tank body will be mixed with the solid components stratified in the vertical direction, resulting in a decline in the simulation effect. And due to various limitations of the experiment, the existing experimental equipment cannot stir the sealed tank body while in the water bath. Therefore, a simulated rumen fermentation device is needed to stir the liquid in the tank body by magnetic stirring to avoid liquid stratification and reduce the accuracy of test results. Content of the Utility Model

[0003] In order to solve the above problems, the present application proposes a simulated rumen fermentation device, which stirs the liquid in the tank body by magnetic stirring to avoid the stratification of liquid and solid and reduce the accuracy of test results.

[0004] The present application is achieved through the following technical solutions:

[0005] The present application proposes a simulated rumen fermentation device, including:

[0006] A water bath tank, the water bath tank is annular, there is an opening at the upper part of the water bath tank, and the lower part of the water bath tank is connected to the ground through a support frame;

[0007] A rotating bracket, the rotating bracket is installed in the middle of the water bath tank, and the rotating shaft of the rotating bracket coincides with the center line of the water bath tank. A plurality of clips are provided on the rotating bracket;

[0008] A fermentation tank, the fermentation tank is located in the water bath tank, and the fermentation tank is connected to the rotating bracket through a clip;

[0009] Among them, a hollow protrusion is provided at the bottom of the water bath tank, a magnetic stirrer is installed inside the protrusion, and magnetic stirring beads are also installed in the fermentation tank.

[0010] Further, a motor is installed on the support frame, the rotating shaft of the motor is connected to the rotating bracket, and the motor is a servo motor.

[0011] Further, the fermentation tank includes a tank body and a cover body. A neck is provided at the upper part of the tank body, and the clip is clamped on the neck. The cover body is connected to the tank body by threads. An exhaust pipe is provided on the cover body. The upper end of the exhaust pipe is connected to the cover body, and the lower end of the exhaust pipe is fixed to the lower part outside the tank body by a buckle. During water bath, the lower end of the exhaust pipe is located below the liquid level.

[0012] Further, a bent part is provided on the rotating bracket, and the bent part bypasses the upper edge of the water bath tank.

[0013] Further, the upper end surface of the protrusion and the bottom of the fermentation tank are made of non-metallic materials, and the distance therebetween is 3 mm to 8 mm.

[0014] Further, 4 to 10 clips are provided on the rotating bracket, and the clips are distributed in a circular array on the outer periphery of the rotating bracket.

[0015] The beneficial effects of the present application: Multiple clips on the rotating bracket simultaneously clamp the fermentation tank and immerse it in the water bath tank to keep it at a constant temperature during the experiment. Magnetic stirring beads are placed in the fermentation tank. Under the rotation of the rotating bracket, it can sequentially pass through the protrusion at the bottom of the water bath tank. After the magnetic stirring beads approach the magnetic stirrer, they can rotate under the drive of the rotating magnetic field, so as to stir the liquid in the fermentation tank while ensuring the sealing of the fermentation tank, imitate rumen peristalsis, avoid liquid stratification at the same time, and improve the accuracy of the experiment. Description of the Drawings

[0016] Figure 1 is a schematic structural diagram of the present utility model;

[0017] Figure 2 is a schematic structural diagram of the rotating bracket of the present utility model;

[0018] Figure 3 is a schematic cross-sectional view of the water bath tank of the present utility model;

[0019] Figure 4 is a schematic structural diagram of the fermentation tank of the present utility model;

[0020] In the figure: 1 - water bath tank, 2 - rotating bracket, 3 - fermentation tank, 4 - support frame, 5 - clip, 6 - protrusion, 7 - magnetic stirrer, 8 - motor, 9 - exhaust pipe, 10 - bent part. Detailed Embodiments

[0021] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions from beginning to end. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

[0022] It should be noted that all the directional indications (such as up, down, left, right, front, back...) in the embodiments of the present application are only used to explain the relative position relationship and movement conditions between components in a specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0023] In addition, the descriptions involving "first", "second", etc. in the present application are only for descriptive purposes, and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of the said features. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present invention.

[0024] As Figures 1 to 4 shown, an embodiment of the present invention provides a simulated rumen fermentation device, including: a water bath tank 1, the water bath tank 1 is annular, there is an opening in the upper part of the water bath tank 1, and the lower part of the water bath tank 1 is connected to the ground through a support frame 4; a rotating bracket 2, the rotating bracket 2 is installed in the middle of the water bath tank 1, and the axis of the rotating bracket 2 coincides with the center line of the water bath tank 1, and a plurality of clips 5 are provided on the rotating bracket 2; a fermentation tank 3, the fermentation tank 3 is located in the water bath tank 1, and the fermentation tank 3 is connected to the rotating bracket 2 through the clip 5; wherein, a hollow protrusion 6 is provided at the bottom of the water bath tank 1, a magnetic stirrer 7 is installed in the protrusion 6, and magnetic stirring beads are also installed in the fermentation tank 3.

[0025] During the experiment, the pre-prepared digestive juice, experimental samples, and magnetic stirring beads are placed into the sterilized fermenter 3 in a carbon dioxide atmosphere. The lid is tightened to keep the inside of the fermenter 3 airtight. Then, the fermenter 3 is placed into the water bath tank 1 and clamped firmly by the clamp 5. Warm water is poured into the water bath tank 1 in advance, and the water bath tank 1 is connected to the heater through a circulation pump. The water in the water bath tank 1 is maintained within the temperature range specified by the experiment through the circulation pump and the heater. After the experiment starts, the motor 8 rotates regularly. Driven by the motor 8, the rotating bracket 2 rotates synchronously, and drives the multiple fermenters 3 on the rotating bracket 2 to pass by the upper part of the protrusion 6 in turn. The upper end surface of the protrusion 6 is close to the bottom of the fermenter 3. When the fermenter 3 is directly above the magnetic stirrer 7, the magnetic stirrer 7 is turned on, and the speed increases from low to high. The magnetic field generated by the magnetic stirrer 7 can drive the magnetic stirring beads in the fermenter 3 to rotate. Thus, under the agitation of the magnetic stirring beads, the digestive juice and the samples in the fermenter 3 are remixed, avoiding the stratification of the digestive juice and improving the accuracy of the experiment. According to the experimental standard, after the fermenter 3 ferments in the water bath for 16 to 96 hours, the water bath is stopped, and the fermenter 3 is opened. By detecting the bottom liquid and residue after fermentation, it can be used to judge the digestion situation of the feed.

[0026] Since the magnetic field generated by the magnetic stirrer 7 needs to be within a 2-cm distance to drive the magnetic stirring beads to rotate, but the water temperature at the bottom of the fermenter 3 is relatively lower than that of the upper layer. Therefore, by setting the protrusion 6, the fermenter 3 can be water-bathed in the middle of the water bath tank 1, and at the same time, when agitation is needed, it can be rotated to the protrusion 6. Since only regular stirring is required in the fermenter 3 to avoid stratification, this device only needs to be equipped with one magnetic stirrer 7 to meet the stirring requirements of all fermenters 3, reducing the cost of the equipment.

[0027] The magnetic stirrer 7 uses a commercially available magnetic stirrer. Its principle is that a motor drives a magnet to generate a rotating magnetic field. When the magnet approaches the magnetic stirring beads, the magnetic stirring beads will rotate under the action of the magnetic force, thus realizing the stirring operation in a sealed tank.

[0028] Specifically, a motor 8 is installed on the support frame 4. The rotating shaft of the motor 8 is connected to the rotating bracket 2. The motor 8 is a servo motor, and the servo motor can control the rotation angle so that the fermenter 3 can stay above the protrusion 6 for 15 to 30 seconds, enabling the liquid in the fermenter 3 to be fully stirred.

[0029] In a specific embodiment, such as Figure 3 、 Figure 4As shown in the figure, the fermentation tank 3 includes a tank body and a cover body. The upper part of the tank body is provided with a neck, and the clip 5 is clamped on the neck so that the fermentation tank 3 can rotate synchronously with the rotating bracket 2. The cover body is connected to the tank body by threads to keep the inside of the fermentation tank 3 airtight, so as to simulate the anaerobic environment in the rumen of ruminants. An exhaust pipe 9 is provided on the cover body. The upper end of the exhaust pipe 9 is connected to the cover body, and the lower end of the exhaust pipe 9 is fixed to the lower part outside the tank body by a buckle. When the digestive juice decomposes the sample, gas will be generated, and the gas is discharged outside the fermentation tank 3 through the exhaust pipe 9 to maintain the internal and external air pressure balance. During water bath, the lower end of the exhaust pipe 9 is located below the liquid level, thus forming a one-way valve, enabling the gas inside the fermentation tank 3 to be freely discharged, while the outside air cannot enter the fermentation tank 3, avoiding the outside air from destroying the anaerobic environment in the fermentation tank 3.

[0030] Preferably, as Figure 2 shown in the figure, the rotating bracket 2 is provided with a bending part 10, and the bending part 10 bypasses the upper edge of the water bath tank 1, so that the clip 5 on the rotating bracket 2 can penetrate deeper into the water bath tank 1, enabling the fermentation tank 3 to fully perform water bath and simulate the constant temperature environment in the rumen of ruminants.

[0031] In a preferred embodiment, the upper end surface of the protrusion 6 and the bottom of the fermentation tank 3 are made of non-metallic materials, and the distance is 3 mm to 8 mm, so that the magnetic stirring beads at the bottom of the fermentation tank 3 are close to the magnetic stirrer 7 installed inside the protrusion 6, and the magnetic stirring beads are rotated through the magnetic field. And the bottom of the fermentation tank 3 is concave, so that when the magnetic stirrer below the protrusion 6 works, it can effectively act on the magnetic stirring beads in the fermentation tank 3 above the protrusion 6 and make them rotate.

[0032] In a specific embodiment, the rotating bracket 2 is provided with 4 to 10 clips 5, and the clips 5 are distributed in a circular array on the outer periphery of the rotating bracket 2. Multiple fermentation tanks 3 can be clamped on the rotating bracket 2 at the same time, so that multiple control groups can be synchronously simulated, enabling the control groups to carry out simulated fermentation under the same conditions.

[0033] Preferably, the volume of the fermentation tank 3 is 300 milliliters to 500 milliliters. The large volume of the fermentation tank 3 can hold more digestive juice and samples, improving the accuracy of the experiment. At the same time, more residues can be collected for subsequent experimental analysis.

[0034] Of course, the present application can also have other various implementation manners. Based on this implementation manner, other implementation manners obtained by those of ordinary skill in the art without any creative labor belong to the scope protected by the present application.

Claims

1. A simulated rumen fermentation device, characterized in that: include: A water bath (1), wherein the water bath (1) is annular, an opening is provided at the top of the water bath (1), and a bottom of the water bath (1) is connected to the ground via a support frame (4); A rotating bracket (2), the rotating bracket (2) being installed in the middle of the water bath (1), and the rotating axis of the rotating bracket (2) coincides with the center line of the water bath (1), and the rotating bracket (2) is provided with a plurality of clips (5); A fermentation tank (3), wherein the fermentation tank (3) is located in the water bath (1), and the fermentation tank (3) is connected to the rotating support (2) via a clamp (5); The bottom of the water bath (1) is provided with a hollow protrusion (6), a magnetic stirrer (7) is installed in the protrusion (6), and magnetic stirring beads are also installed in the fermentation tank (3).

2. A simulated rumen fermentation device according to claim 1, characterized in that: A motor (8) is installed on the support frame (4), the rotating shaft of the motor (8) is connected to the rotating bracket (2), and the motor (8) is a servo motor.

3. The simulated rumen fermentation device according to claim 1, characterized in that: The fermentation tank (3) comprises a tank body and a cover body, the tank body is provided with a neck at the top, the clamp (5) is clamped on the neck, the cover body is connected to the tank body via a thread, an exhaust pipe (9) is provided on the cover body, the upper end of the exhaust pipe (9) is connected to the cover body, and the lower end of the exhaust pipe (9) is fixed to the lower part of the outer side of the tank body via a buckle, and when in a water bath, the lower end of the exhaust pipe (9) is located below the liquid surface.

4. The simulated rumen fermentation device according to claim 1, characterized in that: The rotating bracket (2) is provided with a bending portion (10), and the bending portion (10) bypasses the upper edge of the water bath (1).

5. The simulated rumen fermentation device according to claim 1, characterized in that: The upper end surface of the protrusion (6) and the bottom of the fermentation tank (3) are made of non-metallic material, and the distance between them is 3 mm to 8 mm.

6. The simulated rumen fermentation device according to claim 1, characterized in that: The rotating bracket (2) is provided with 4 to 10 clips (5), and the clips (5) are distributed on the periphery of the rotating bracket (2) in a ring array.

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