293 cell culture device

Abstract

The utility model discloses a 293 cell culture device which comprises a base, wherein a rack and a culture seat are arranged on the base; a plurality of groups of mounting ports are formed in the culture base, and a clamping structure for synchronously fixing a plurality of groups of culture dishes is also arranged on the culture base; a transverse driving structure for transversely oscillating the culture dishes is arranged on the rack; the transverse driving structure comprises a connecting rod, the connecting rod is arranged on one side of the culture base, and one end of the connecting rod is movably arranged in the rack; the rack is further provided with a transmission box, and a transmission assembly used for driving the connecting rod to move transversely is arranged in the transmission box. A guide rod is arranged on the other side of the culture base, a guide sleeve is arranged on the machine frame, and the guide rod is movably arranged in the guide sleeve. According to the utility model, a plurality of groups of culture dishes can be transversely oscillated in a continuous reciprocating manner, so that nutrient solution and cells can be fully mixed, the cell culture efficiency is improved, and the culture dishes can be fixed simply, conveniently and quickly.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and particularly relates to a 293 cell culture device. Background Technique

[0002] 293 cells are a human renal epithelial cell line with multiple derivative strains, such as HEK293, 293T / 17, etc. They all originate from human embryonic kidney cells, which rarely express endogenous receptors required for extracellular ligands and are relatively easy to transfect. It is a very commonly used cell line for expressing exogenous genes in research. Usually, a cell culture device is needed to culture 293 cells in the laboratory.

[0003] At present, during the cell culture process of the cell culture device, the nutrient solution in the culture dish cannot be horizontally oscillated with 293 cells, and the nutrient solution and 293 cells cannot be quickly mixed, which affects the 293 cell culture efficiency. At the same time, it cannot synchronously fix multiple groups of culture dishes, and multiple groups of culture dishes need to be fixed sequentially, and the fixing operation is cumbersome. Therefore, we propose to develop a new 293 cell culture device. Content of the Utility Model

[0004] The purpose of the utility model is to provide a 293 cell culture device to solve the problems in the above background technique that during the cell culture process of the current cell culture device, the nutrient solution in the culture dish cannot be horizontally oscillated with 293 cells, the nutrient solution and 293 cells cannot be quickly mixed, which affects the 293 cell culture efficiency, and at the same time, it cannot synchronously fix multiple groups of culture dishes, and multiple groups of culture dishes need to be fixed sequentially, and the fixing operation is cumbersome.

[0005] To achieve the above purpose, the utility model provides the following technical solutions:

[0006] A 293 cell culture device, comprising:

[0007] A base, on which a frame and a culture base are provided; multiple mounting ports are provided on the culture base, and a clamping structure for synchronously fixing multiple groups of culture dishes is also provided on the culture base;

[0008] A horizontal driving structure for horizontally oscillating the culture dish is provided on the frame;

[0009] The horizontal driving structure includes a connecting rod, the connecting rod is arranged on one side of the culture base, and one end of the connecting rod is movably arranged in the frame;

[0010] A transmission box is also provided on the frame, and a transmission component for driving the connecting rod to move horizontally is arranged in the transmission box;

[0011] On the other side of the culture base, a guide rod is provided. A guide sleeve is provided on the frame, and the guide rod is movably arranged in the guide sleeve. A return spring is also arranged in the guide sleeve, and the return spring is connected to the guide rod.

[0012] In one embodiment, the driving structure includes a cam. The cam is arranged on a driving shaft. The driving shaft is rotatably arranged in a transmission box and is connected to a driving motor located on the transmission box. By the rotation of the cam, the connecting rod can be driven to move horizontally back and forth.

[0013] In one embodiment, an arc-shaped top block is arranged at one end of the connecting rod. The cam is intermittently connected to the arc-shaped top block, which is convenient for the rotation of the cam to drive the connecting rod to move.

[0014] In one embodiment, a guide slider is arranged at the bottom of the culture base. The guide slider is slidably arranged in a guide groove. The guide groove is arranged on the base, which improves the stability of the culture base when it moves.

[0015] In one embodiment, the clamping structure includes a mounting frame. The mounting frame is arranged on the culture base. A first electric push rod is arranged in the mounting frame. The piston rod of the first electric push rod is connected to a support plate. Support rods are equidistantly arranged on the support plate. One end of each support rod extends into a groove in turn. The grooves are arranged in corresponding mounting openings, and multiple groups of culture dishes can be fixed synchronously.

[0016] In one embodiment, a pressing plate is further arranged at one end of the support rod. The pressing plate is arranged in the groove, which further improves the firmness of fixing multiple groups of culture dishes.

[0017] In one embodiment, a second electric push rod is also arranged in the culture base. The piston rod of the second electric push rod is connected to a top plate. A discharging assembly for jacking up the culture dish is arranged on the top plate.

[0018] In one embodiment, the discharging assembly includes a top rod. The top rod is arranged on the top plate. A jacking plate is arranged on the top rod. The jacking plate is received in a bottom receiving opening. The bottom receiving opening is arranged at the bottom of the mounting opening, and multiple groups of culture dishes can be automatically jacked up, which is convenient for taking and placing the culture dishes.

[0019] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0020] (1) The present utility model can perform continuous reciprocating horizontal oscillation on multiple groups of culture dishes, which is convenient for the full mixing of nutrient solution and cells, improves the cell culture efficiency, and ensures the survival rate.

[0021] (2) The present utility model can synchronously clamp and fix multiple groups of culture dishes, and at the same time, the fixing is firm, and the fixing operation of the culture dishes is simple and fast. Brief Description of the Drawings

[0022] Figure 1 It is a schematic structural diagram of an embodiment of the present utility model.

[0023] Figure 2 It is Figure 1 a half-sectional structural diagram of

[0024] Figure 3 It is Figure 2 an enlarged structural diagram of part A in

[0025] Figure 4 It is Figure 2 an enlarged structural diagram of part B in

[0026] Figure 5 It is a half-sectional structural diagram of the clamping structure in an embodiment.

[0027] Figure 6 It is Figure 5 an enlarged structural diagram of part C in Detailed Description of the Embodiments

[0028] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0029] Please refer to Figures 1-3 , the present utility model provides a 293 cell culture device, including a base 5, a frame 4 provided on the base 5, a culture base 6 provided on the base 5, and three sets of mounting ports 3 are further provided on the culture base 6. Specifically, the number of the mounting ports 3 is not limited to three sets. A lateral driving structure 1 for horizontally oscillating the culture dish is provided on the frame 4.

[0030] The lateral driving structure 1 includes a connecting rod 102. The connecting rod 102 is provided on one side of the culture base 6 and is movably arranged in the frame 4. A transmission box 111 is further provided on the frame 4. A transmission component for driving the connecting rod 102 to move horizontally is provided in the transmission box 111. A guide rod 105 is provided on the other side in the culture base 6. The guide rod 105 is movably arranged in a guide sleeve 107. The guide sleeve 107 is provided on the frame 4. A return spring 106 is further provided in the guide sleeve 107. The return spring 106 is connected to the guide rod 105.

[0031] The transmission assembly includes a cam 109, which is arranged on a drive shaft 108. The drive shaft 108 is rotatably arranged in a transmission box 111, and the drive shaft 108 is also connected to a drive motor 101 located on the transmission box 111. By the rotation of the cam 109, the connecting rod 102 can be driven to move horizontally back and forth. The cam 109 is intermittently connected to an arc-shaped top block 110. The arc-shaped top block 110 is arranged at one end of the connecting rod 102, which is convenient for the rotation of the cam 109 to drive the connecting rod 102 to move.

[0032] In specific applications, first, a plurality of culture dishes are placed in the installation opening 3, and then the plurality of culture dishes are fixed in the installation opening 3 through the clamping structure 2. Then, the drive motor 101 drives the drive shaft 108 to rotate, and the drive shaft 108 drives the cam 109 to rotate. When the cam 109 is in contact with the arc-shaped top block 110, with the rotation of the cam 109, the arc-shaped top block 110 is driven to move horizontally. The arc-shaped top block 110 drives the connecting rod 102 to move horizontally, and the connecting rod 102 drives the culture seat 6 to move horizontally. The culture seat 6 drives the guide rod 105 to move in the guide sleeve 107 and compresses the return spring 106. When the cam 109 is separated from the arc-shaped top block 110, the return spring 106 drives the guide rod 105 to quickly return, and then the culture seat 6 quickly returns. With the intermittent contact between the cam 109 and the arc-shaped top block 110, the culture seat 6 can drive a plurality of culture dishes to oscillate horizontally synchronously, which is convenient for the nutrient solution to be quickly mixed with 293 cells.

[0033] Furthermore, a guide slider 104 is arranged at the bottom of the culture seat 6. The guide slider 104 is slidably arranged in a guide groove 103. The guide groove 103 is arranged on the base 5. During the movement of the culture seat 6, the guide slider 104 is driven to move in the guide groove 103, which improves the stability of the culture seat 6 during movement.

[0034] As a specific embodiment of the present application, please refer to Figure 2 、 Figure 5 and Figure 6 , the culture seat 6 is also provided with a clamping structure 2 for synchronously fixing a plurality of culture dishes. The clamping structure 2 includes a mounting frame 21. The mounting frame 21 is arranged on the culture seat 6. A first electric push rod 23 is arranged in the mounting frame 21. The piston rod of the first electric push rod 23 is connected to a support plate 24. Support rods 22 are arranged at equal intervals on the support plate 24. One ends of the support rods 22 respectively extend into grooves 25. The grooves 25 are sequentially arranged in the corresponding installation openings 3, and a plurality of culture dishes can be synchronously fixed. A pressing plate 26 is also arranged at one end of the support rod 22. The pressing plate 26 is arranged in the groove 25, which further improves the firmness of the fixation of a plurality of culture dishes.

[0035] Specifically, first, three groups of culture dishes are placed in the corresponding installation ports 3. Then, the support plate 24 is driven to move by the first electric push rod 23. The support plate 24 simultaneously drives the three support rods 22 to move. The movement of the support rods 22 drives the pressing plate 26 to move. When the pressing plate 26 is in contact with the culture dish, the pressing plate 26 drives the culture dish to move and makes the culture dish contact with the other side in the installation port 3, thereby synchronously fixing the three groups of culture dishes in the installation port 3.

[0036] In one embodiment, please refer to Figure 2 and Figure 3 , a second electric push rod 61 is further provided in the culture base 6. The piston rod of the second electric push rod 61 is the top plate 62. The top plate 62 is provided with a discharging assembly for jacking up the culture dish. The discharging assembly includes a jacking rod 63. The jacking rod 63 is provided on the top plate 62. An elevating plate 64 is provided on the jacking rod 63. The elevating plate 64 is received in the bottom receiving port 65. The bottom receiving port 65 is provided at the bottom of the installation port 3, which can automatically jack up multiple groups of culture dishes, facilitating the picking and placing of the culture dishes.

[0037] When the 293 cells in the culture dish are cultured, the culture dish is released. The second electric push rod 61 drives the top plate 62 to jack up. The top plate 62 drives the jacking rod 63 to move upward. The upward movement of the jacking rod 63 drives the elevating plate 64 to move upward. As the elevating plate 64 moves, the three groups of culture dishes can be synchronously jacked up, facilitating the experimenter to pick and place the culture dishes.

[0038] The first electric push rod 23, the second electric push rod 61, and the drive motor 101 in this application are all electrically connected to the control panel 7 on the transmission box 111, and the models of the first electric push rod 23 and the second electric push rod 61 are both DT50.

[0039] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A 293 cell culture device, characterized in that: include: A base, wherein a frame and a culture seat are arranged on the base; the culture seat is provided with a plurality of mounting ports, and the culture seat is also provided with a clamping structure for synchronously fixing a plurality of groups of culture dishes; The frame is provided with a transverse driving structure for transversely oscillating the culture dish; The lateral driving structure includes a connecting rod, which is arranged on one side of the culture seat, and one end of the connecting rod is movably arranged in the frame; A transmission box is also arranged on the frame, and a transmission assembly for driving the connecting rod to move horizontally is arranged in the transmission box; A guide rod is arranged on the other side of the culture seat, a guide sleeve is arranged on the frame, and the guide rod is movably arranged in the guide sleeve; a reset spring is also arranged in the guide sleeve, and the reset spring is connected to the guide rod.

2. The 293 cell culture device according to claim 1, characterized in that: The driving structure comprises a cam, and the cam is arranged on a driving shaft. The driving shaft is rotatably arranged in a transmission box, and the driving shaft is connected to a driving motor located on the transmission box.

3. The 293 cell culture device according to claim 2, characterized in that: An arc-shaped top block is arranged at one end of the connecting rod, and the cam is intermittently connected to the arc-shaped top block.

4. The 293 cell culture device according to claim 1, characterized in that: The base is also provided with a guide groove, and the bottom of the culture seat is provided with a guide sliding block, and the guide sliding block is slidably arranged in the guide groove.

5. The 293 cell culture device according to claim 1, characterized in that: The clamping structure includes a mounting frame, which is arranged on a culture seat; a first electric push rod is arranged in the mounting frame, and a piston rod of the first electric push rod is connected to a support plate, and support rods are equidistantly arranged on the support plate (24); a groove is arranged in each of the mounting openings, and one end of the support rod extends into the groove.

6. The 293 cell culture device according to claim 5, characterized in that: A pressing plate is also arranged at one end of the support rod, and the pressing plate is arranged in the groove.

7. The 293 cell culture device according to claim 1 or 5, characterized in that: A second electric push rod is also arranged in the culture seat, a top plate is arranged on the piston rod of the second electric push rod, and a discharge assembly for lifting the culture dish is arranged on the top plate.

8. The 293 cell culture device according to claim 7, characterized in that: The unloading assembly includes a push rod, which is arranged on the top plate. A lifting plate is arranged on the push rod, and the lifting plate is received in a bottom receiving opening, and the bottom receiving opening is arranged at the bottom of the installation opening.

Images