Stem cell culture perfusion device

Abstract

The utility model provides a stem cell culture perfusion device, and belongs to the technical field of stem cell culture. Comprising a box body, and a fixed seat is vertically and fixedly connected to the center of the inner bottom wall; the box cover is fixed at the top of the box body, a fixing rod is vertically and fixedly connected to the center of the bottom of the box cover, and the fixing rod and the fixing seat are matched and mutually butted; the storage tray is used for mounting culture test tubes, is positioned in the box body and is clamped with the fixed seat; the sealing disc is in threaded connection with the fixing rod in a matched mode and is in sealing connection with the top of the culture test tube in a normal state, and a through hole is formed in the sealing disc. According to the utility model, the plurality of perfusion ports are arranged to be matched with the plurality of culture test tubes mounted on the storage tray, so that the culture solution perfusion work can be performed on the plurality of culture test tubes at one time, and the working efficiency is improved; the sealing disc which is in threaded connection and is of a disc-shaped structure is used for sealing the culture test tube, and the sealing performance of the stem cell culture environment is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture, in particular to a perfusion device for stem cell culture. Background Art

[0002] Stem cells are a class of cells with unlimited or immortal self-renewal ability, and can produce at least one type of highly differentiated progeny cells. Over the years, the definition of stem cells has been continuously revised and defined from different aspects. Currently, most biologists and medical scientists believe that stem cells are a class of cells derived from embryos, fetuses or adults with the ability of unlimited self-renewal and proliferation and differentiation under certain conditions, which can produce daughter cells with the same phenotype and genotype as themselves, can also produce specialized cells that make up the tissues and organs of the body, and can also differentiate into progenitor cells.

[0003] When the current stem cell tissue fluid is used for detection and other medical purposes, it needs to be cultivated with nutrient solution, and a batch of stem cell test tubes requires a certain amount of nutrient solution for cultivation. After retrieval, the Chinese patent with the publication number CN220467991U discloses a perfusion device suitable for stem cell culture, including a base, a box body is installed at the top of the base, a cross plate is installed inside the box body, a culture bottle is installed at the bottom of the cross plate, a telescopic plate is movably connected inside the cross plate, through holes and grooves are respectively formed on the surfaces of the cross plate and the telescopic plate in a penetrating manner, and an electric push rod is installed on one side of the box body; through the cooperation between the electric push rod and the telescopic plate, the perfusion of the culture bottle can be carried out at one time, reducing the tediousness of manually perfusing multiple culture bottles in sequence. At the same time, the cooperation between the electric push rod and the telescopic plate also facilitates closing the opening of the culture bottle after perfusion, ensuring the sealing performance of the culture bottle after perfusion, and effectively improving the linkage of the perfusion device.

[0004] However, in the above solution, the sealing of the culture bottle is achieved by the cooperation of the cross plate and the telescopic plate connected by sliding. Since it is necessary to ensure the smooth sliding of the cross plate and the telescopic plate, there is inevitably a certain gap between the two, resulting in poor actual sealing effect on the culture bottle. Therefore, the present application provides a perfusion device for stem cell culture to meet the requirements. Summary of the Utility Model

[0005] The technical problem to be solved by the utility model is to provide a perfusion device for stem cell culture to solve the problem that the sealing of the culture bottle is achieved by the cooperation of the cross plate and the telescopic plate connected by sliding in the prior art. Since it is necessary to ensure the smooth sliding of the cross plate and the telescopic plate, there is inevitably a certain gap between the two, resulting in poor actual sealing effect on the culture bottle.

[0006] To solve the above technical problem, the utility model provides the following technical solutions:

[0007] A stem cell culture perfusion device, comprising: a box body, with a fixing seat vertically and fixedly connected to the center of the inner bottom wall; a box cover, fixed to the top of the box body, and the box body and the box cover cooperate with each other to form an internal closed cavity structure. A fixing rod is vertically and fixedly connected to the center of the bottom of the box cover. The fixing rod is docked with the fixing seat in cooperation. An external thread groove is provided on the outer side wall of the fixing rod near the bottom; a placement tray, used for installing culture test tubes, is located inside the box body and is snap-connected to the fixing seat; a sealing disk, threadedly connected with the fixing rod, and is set to be hermetically connected to the top of the culture test tube under normal conditions. A through hole is provided on the sealing disk.

[0008] Preferably, an operation window is provided on the side wall of the box body for manually replacing the sealing disk.

[0009] Preferably, a perfusion port is provided on the inner top of the box cover for perfusing the culture medium.

[0010] Preferably, it further includes a liquid storage tank, fixed to the top of the box cover. A liquid supplement port is provided on the top of the liquid storage tank for supplementing nutrient solution. The liquid storage tank is communicated with the perfusion port through a catheter, and a peristaltic pump is provided on the catheter for pumping the culture medium.

[0011] Preferably, a positioning groove is provided on the top of the fixing seat, and a positioning rod is provided at the bottom of the fixing rod. The positioning rod is connected with the positioning groove in a plug-in connection.

[0012] Preferably, a clamping block is provided in the middle section of the fixing seat, and a clamping groove is provided on the placement tray. The clamping groove is snap-connected with the clamping block.

[0013] Preferably, it further includes a limiting plate, arranged at the bottom end of the clamping block, and the limiting plate protrudes from the outer side wall of the clamping block for supporting the placement tray.

[0014] Preferably, the placement tray is provided with insertion holes, and a boss is provided above the insertion holes for supporting and fixing the culture test tubes.

[0015] Preferably, it further includes a connecting seat, fixed to the center of the sealing disk. The connecting seat is in a cylindrical structure, and an internal thread groove is provided on the inner side wall of the connecting seat for threadedly connecting with the external thread groove. A driven gear is provided on the outside of the connecting seat.

[0016] Preferably, it further includes a motor, fixedly connected inside the box cover. The output end of the motor is fixedly connected with a driving gear, and the driving gear is meshed with the driven gear in cooperation.

[0017] Compared with the prior art, the present utility model has at least the following beneficial effects:

[0018] In the above solution, by providing a plurality of perfusion ports in cooperation with a plurality of culture test tubes installed on the placement tray, the perfusion work of the culture medium for a plurality of culture test tubes can be achieved at one time, improving the work efficiency; a sealing disc with a threaded connection and a disc-shaped structure is used to seal the culture test tubes, ensuring the sealing of the stem cell culture environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings incorporated herein and constituting a part of the specification illustrate embodiments of the present disclosure and, together with the specification, are further used to explain the principles of the present disclosure and enable those skilled in the relevant art to implement and use the present disclosure.

[0020] Figure 1 Schematic diagram of the overall structure of the utility model;

[0021] Figure 2 Schematic diagram of the lid structure of the utility model;

[0022] Figure 3 Schematic diagram of the sealing disc structure of the utility model;

[0023] Figure 4 Schematic diagram of the box body structure of the utility model;

[0024] Figure 5 Schematic diagram of the fixed seat structure of the utility model;

[0025] Figure 6 Schematic diagram of the placement tray structure of the utility model.

[0026] In the figure: 1, box body; 2, lid; 3, liquid storage tank; 4, liquid replenishing port; 5, conduit; 6, peristaltic pump; 7, motor; 8, driving gear; 9, perfusion port; 10, fixing rod; 11, external thread groove; 12, positioning rod; 13, sealing disc; 14, connecting seat; 15, driven gear; 16, internal thread groove; 17, through hole; 18, operation window; 19, fixed seat; 20, clamping block; 21, limiting plate; 22, positioning groove; 23, placement tray; 24, card slot; 25, jack; 26, boss; 27, culture test tube.

[0027] As shown in the figure, in order to clearly implement the structure of the embodiments of the present utility model, specific structures and devices are marked in the figure, but this is only for schematic needs and is not intended to limit the present utility model to this specific structure, device and environment. According to specific needs, those of ordinary skill in the art can adjust or modify these devices and environments, and the adjustments or modifications made are still included within the scope of the appended claims. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0028] The following will combine the accompanying drawings and specific embodiments to describe in detail a stem cell culture perfusion device provided by the present utility model. At the same time, it should be noted here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments. For some well-known technologies, those skilled in the art can also adopt other alternative methods for implementation; moreover, the accompanying drawings are only for more specifically describing the embodiments, and are not intended to specifically limit the present utility model.

[0029] As Figure 1 - Figure 6 shown, an embodiment of the present utility model provides a stem cell culture perfusion device, including: a box body 1, with a fixing seat 19 vertically and fixedly connected to the center of the inner bottom wall; a box cover 2, fixed on the top of the box body 1, and the box body 1 and the box cover 2 cooperate with each other to form an internally enclosed cavity structure. A fixing rod 10 is vertically and fixedly connected to the center position of the bottom of the box cover 2. The fixing rod 10 is in butt joint with the fixing seat 19. An external thread groove 11 is provided on the outer side wall of the fixing rod 10 near the bottom. A perfusion port 9 is provided on the inner top of the box cover 2 for perfusing the culture medium; a placement tray 23 for installing the culture test tube 27, and the placement tray 23 is located inside the box body 1 and is snap-connected to the fixing seat 19; a sealing disk 13, threadedly connected with the fixing rod 10, and is set to be hermetically connected to the top of the culture test tube 27 under normal conditions. A through hole 17 is provided on the sealing disk 13.

[0030] Among them, there are multiple culture test tubes 27 and through holes 17, and the set numbers are the same. In this embodiment, seven are provided with reference to the illustration. The through holes 17 are distributed in an octagonal shape on the sealing disk 13, and the culture test tubes 27 are installed in an octagonal shape on the placement tray 23. Specifically, one orientation of the octagonal structure formed by the culture test tubes 27 on the placement tray 23 is used to open a card slot 24, and one orientation of the octagonal structure formed by the through holes 17 on the sealing disk 13 is not punched (for the specific structure, please refer to Fig. storage tank 3 and Fig. peristaltic pump 6). Further, the number of perfusion ports 9 is also seven, distributed in an octagonal shape, and one orientation of the octagonal structure does not have a perfusion port 9. During assembly, directly below each perfusion port 9, there is a corresponding culture test tube 27. When performing perfusion work through the through hole 17, by rotating the sealing disk 13, it moves to the middle position between the relatively arranged perfusion port 9 and the culture test tube 27 to ensure the passage of the culture medium.

[0031] By providing multiple perfusion ports 9 and cooperating with multiple culture test tubes 27 installed on the placement tray 23, the perfusion work of the culture medium for multiple culture test tubes 27 can be realized at one time, improving the work efficiency; the sealing disk 13, which is threadedly connected and has a disk-shaped structure, is used to seal the culture test tube 27 to ensure the sealing of the stem cell culture environment.

[0032] As Figure 4As shown, an operation window 18 is provided on the side wall of the box body 1 for manually replacing the sealing disc 13.

[0033] Among them, the width of the operation window 18 is greater than the diameter of the storage tray 23, ensuring that the storage tray 23 can pass horizontally through the operation window 18. One side of the operation window 18 is rotatably connected to a cover plate through a hinge. The cover plate has a curved surface structure, and the other end of the cover plate is closed to the operation window 18 through a locking structure such as magnetic attraction or a buckle. When the cover plate closes the operation window 18, the entire outer side wall of the box body 1 has a cylindrical structure.

[0034] As Figure 1 shown, it also includes a liquid storage tank 3 fixed on the top of the box cover 2. A liquid replenishment port 4 is provided on the top of the liquid storage tank 3 for supplementing nutrient solution to ensure sufficient culture solution inside the liquid storage tank 3. The liquid storage tank 3 is connected to the perfusion port 9 through a conduit 5. The conduit 5 is made of a latex hose, and a peristaltic pump 6 is provided on the conduit 5 for pumping the culture solution. The peristaltic pump 6 realizes liquid transportation by bending and squeezing the conduit 5.

[0035] Among them, the liquid storage tank 3 has an annular structure, and both the conduit 5 and the peristaltic pump 6 are arranged at the central cavity position of the liquid storage tank 3. The conduit 5 passes through the center position of the top wall of the box cover 2 and is connected to the perfusion port 9, and the connection part is sealed to ensure the airtightness of the transportation channel.

[0036] As Figure 2 and Figure 5 shown, a positioning groove 22 is provided at the top of the fixing seat 19, and a positioning rod 12 is provided at the bottom of the fixing rod 10. The positioning rod 12 is connected to the positioning groove 22 in a matching socket connection.

[0037] In this embodiment, the radial cross-sectional shapes of both the positioning rod 12 and the positioning groove 22 are rectangular to ensure the docking angle between the box body 1 and the box cover 2. When installing the storage tray 23 subsequently, a culture test tube 27 is corresponding to the position directly below each perfusion port 9.

[0038] As Figure 5 and Figure 6 shown, a clamping block 20 is provided in the middle section of the fixing seat 19, and a clamping groove 24 is provided on the storage tray 23. The clamping groove 24 is engaged with the clamping block 20.

[0039] Among them, by symmetrically opening two notches on the side wall of the fixing seat 19, a clamping block 20 structure with parallel side walls is formed. The clamping groove 24 presents a notch structure extending linearly from the center position of the storage tray 23 to the outside, and is set so that when the clamping groove 24 is engaged with the clamping block 20, the central axes of the storage tray 23 and the fixing seat 19 coincide.

[0040] As Figure 5 shown, it also includes a limiting plate 21 provided at the bottom end position of the clamping block 20, and the limiting plate 21 protrudes from the outer side wall of the clamping block 20 for supporting the storage tray 23.

[0041] The limiting plate 21 provides additional support for the placing tray 23, further enhancing the stability of the placing tray 23. Especially during the perfusion process, it can effectively prevent shaking caused by liquid impact and protect the stem cells in the culture tube 27 from being affected.

[0042] like Figure 6 As shown, a plug hole 25 is provided on the placement plate 23 , and a boss 26 is provided above the plug hole 25 for supporting and fixing a culture tube 27 .

[0043] Among them, the inner wall of the hole 25 is slidably connected with the outer wall of the culture tube 27, the boss 26 is a truncated cone structure, and a sliding groove with a smooth transition to the inner wall of the hole 25 is provided at the center position, which is also slidably connected with the culture tube 27. Through the setting of the boss 26, not only the structural stability of the installation of the culture tube 27 is improved, but also the culture tube 27 is lifted, so that the culture tube 27 and the perfusion port 9 are close to each other, avoiding the problem of splashing of culture fluid due to excessive drop during perfusion operation.

[0044] like Figure 3 As shown, it also includes a connecting seat 14, which is fixed at the center of the sealing disk 13. The connecting seat 14 is a cylindrical structure, and an internal thread groove 16 is opened on the inner wall of the connecting seat 14 for threaded connection with the external thread groove 11. A driven gear 15 is provided on the outer side of the connecting seat 14.

[0045] Through the threaded connection between the sealing disk 13 and the fixing rod 10, when the sealing disk 13 is rotated, not only the position of the through hole 17 and the top opening of the culture tube 27 are staggered, but also the downward rotation force is generated due to the threaded connection, so that the bottom of the sealing disk 13 and the top opening of the culture tube 27 are close to each other and abutted, thereby improving the sealing effect of the top opening of the culture tube 27.

[0046] like Figure 2 As shown, it also includes a motor 7, which is fixedly connected inside the box cover 2, and the output end of the motor 7 is fixedly connected to a driving gear 8, and the driving gear 8 is meshed and connected with a driven gear 15.

[0047] The motor 7 drives the gear 8 to rotate, and then cooperates with the meshing connection between the driving gear 8 and the driven gear 15, so as to realize the rotation drive of the sealing disk 13 as a whole.

[0048] The technical solution provided by the utility model, when actually used, first, ensure that a sufficient amount of culture fluid has been added to the liquid storage tank 3, and replenish it through the liquid infusion port 4. Check whether the peristaltic pump 6 is connected normally and ensure that it is in standby mode. Check the motor 7 and its control system to ensure that the motor 7 can be driven normally. After confirmation, insert the culture tubes 27 containing stem cells into the sockets 25 on the placement tray 23 one by one, and ensure that the top protrusions of the culture tubes 27 are stably placed on the bosses 26 and firmly fixed. Open the cover of the operating window 18 on the side wall of the box body 1, and clamp the placement tray 23 on the block 20 of the fixing seat 19. At this time, the limit plate 21 supports the bottom of the placement tray 23. Close the cover of the operating window 18 and lock it to ensure sealing. Subsequently, the motor 7 is started to drive the sealing disk 13 to rotate to the initial sealing position, ensuring that the through hole 17 at the bottom of the sealing disk 13 is not aligned with the top opening of any culture tube 27, and the sealing effect is verified to ensure that the top of the culture tube 27 is effectively sealed. When the culture fluid perfusion operation is required, the motor 7 is started, and the motor 7 drives the driven gear 15 to rotate through the driving gear 8, thereby driving the sealing disk 13 to rotate as a whole. As the sealing disk 13 rotates, the through holes 17 move one by one to the positions relative to the perfusion port 9 and the culture tube 27, and the sealing is performed. As the disk 13 rotates, it is relatively raised under the action of the thread groove, so that the bottom of the sealing disk 13 does not produce relative friction with the top of the culture tube 27 during the rotation of the sealing disk 13. Subsequently, the peristaltic pump 6 is started to pump the culture fluid, and the culture fluid is discharged from the perfusion port 9, passed through the through hole 17 and poured into the culture tube 27, thereby realizing automatic perfusion of the culture fluid. After the perfusion operation is completed, the motor 7 is reversed to drive the sealing disk 13 to rotate in the opposite direction, so that the through hole 17 and the culture tube 27 are staggered, and the sealing disk 13 is slightly pressed down and abuts against the top of the culture tube 27 to achieve sealing.

[0049] The present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention. In order to make the public have a thorough understanding of the present invention, specific details are described in detail in the above preferred embodiments of the present invention, and those skilled in the art can fully understand the present invention without the description of these details. In addition, in order to avoid unnecessary confusion about the essence of the present invention, well-known methods, processes, procedures, components and circuits are not described in detail.

[0050] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A stem cell culture perfusion device, characterized in that: include: The box body (1) has a fixing seat (19) vertically fixedly connected to the center of the inner bottom wall; A box cover (2) is fixed on the top of the box body (1), and the box body (1) and the box cover (2) cooperate with each other to form an internal closed cavity structure. A fixing rod (10) is vertically fixedly connected to the center of the bottom of the box cover (2), and the fixing rod (10) and the fixing seat (19) cooperate to butt against each other. An external thread groove (11) is provided on the outer side wall of the bottom of the fixing rod (10); A storage tray (23) for mounting a culture tube (27), wherein the storage tray (23) is located inside the box body (1) and is snap-connected to the fixing seat (19); The sealing disk (13) is threadedly connected to the fixing rod (10) and is configured to be sealed with the top of the culture tube (27) under normal conditions. A through hole (17) is provided on the sealing disk (13).

2. The stem cell culture perfusion device according to claim 1, characterized in that: An operating window (18) is provided on the side wall of the box body (1) for manually replacing the sealing disk (13).

3. The stem cell culture perfusion device according to claim 1, characterized in that: The top of the box cover (2) is provided with a perfusion port (9) for perfusing culture fluid.

4. The stem cell culture perfusion device according to claim 3, characterized in that: It also includes a liquid storage tank (3) fixed on the top of the box cover (2), and a liquid replenishment port (4) is provided on the top of the liquid storage tank (3) for replenishing nutrient solution. The liquid storage tank (3) is connected to the perfusion port (9) through a conduit (5), and a peristaltic pump (6) is provided on the conduit (5) for pumping culture solution.

5. The stem cell culture perfusion device according to claim 1, characterized in that: A positioning groove (22) is provided on the top of the fixing seat (19), a positioning rod (12) is provided on the bottom of the fixing rod (10), and the positioning rod (12) is connected to the positioning groove (22) in a socket-and-socket manner.

6. The stem cell culture perfusion device according to claim 1, characterized in that: A clamping block (20) is provided in the middle section of the fixing seat (19), and a clamping slot (24) is provided on the storage tray (23), wherein the clamping slot (24) is engaged with the clamping block (20).

7. The stem cell culture perfusion device according to claim 6, characterized in that: It also includes a limiting plate (21) disposed at the bottom end of the clamping block (20), and the limiting plate (21) protrudes from the outer side wall of the clamping block (20) and is used to support the storage tray (23).

8. The stem cell culture perfusion device according to claim 1, characterized in that: The placement plate (23) is provided with an insertion hole (25), and a boss (26) is provided above the insertion hole (25) for supporting and fixing the culture test tube (27).

9. The stem cell culture perfusion device according to claim 1, characterized in that: It also includes a connecting seat (14) fixed at the center of the sealing disk (13); the connecting seat (14) is in a cylindrical structure, and an internal thread groove (16) is provided on the inner side wall of the connecting seat (14) for threaded connection with the external thread groove (11); and a driven gear (15) is provided on the outer side of the connecting seat (14).

10. The stem cell culture perfusion device according to claim 9, characterized in that: It also includes a motor (7) fixedly connected inside the box cover (2), the output end of the motor (7) being fixedly connected to a driving gear (8), and the driving gear (8) being meshingly connected with the driven gear (15).

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