Rhododendron lapponicum somatic embryo culture device

Abstract

The utility model relates to the technical field of plant cell culture equipment, and provides a rhododendron lapponicum somatic embryo culture device which comprises a cell culture box body, a sealing box door is rotatably mounted on the front wall of the cell culture box body, and an observation window is mounted on the sealing box door; the device comprises a mounting plate, a rotating disc is rotatably mounted in the center of the mounting plate, a plurality of culture dish placing seats are symmetrically and rotatably mounted on the top wall of the rotating disc, a bottom cover is fixed to the bottom wall of the mounting plate, a grooved wheel mechanism for driving the rotating disc to rotate is mounted in the bottom cover, and a rotating assembly for driving the culture dish placing seats to rotate is mounted in the rotating disc; the sealing assembly is fixed on the top wall of the inner cavity of the cell culture box body; according to the technical scheme, the problems that a plurality of culture dishes are generally placed in a cell culture box in use in the prior art, so that the culture dishes in the cell culture box need to be taken out and observed one by one when needing to be observed, the use is inconvenient, the observation time is wasted, and the operation is tedious are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of plant cell culture equipment, and particularly relates to a somatic embryo culture device for Rhododendron lapponicum. Background Technique

[0002] Rhododendron lapponicum is an evergreen dwarf shrub of the genus Rhododendron in the family Ericaceae. The branches are dense, prostrate or straight. The young branches are densely covered with rusty brown scales and mixed with fine hairs. The leaves are often scattered at the top of the branches, leathery, oblong-elliptic to ovate-elliptic, or oblong-obovate, densely covered with almost adjacent or overlapping grayish-white scales. The inflorescence is terminal and umbellate, with red or purple lobes, ovate-triangular or nearly round, covered with sparse or dense scales, and the margin is covered with long ciliate or occasionally scales. The capsule is oblong-ovate, densely covered with scales.

[0003] During the culture process of somatic embryos of Rhododendron lapponicum, they are generally cultured in a cell culture incubator. However, when the existing cell culture incubator is in use, multiple culture dishes are generally placed. Therefore, when it is necessary to observe the internal culture dishes, they need to be taken out one by one for observation, which is inconvenient to use, wastes the observation time, and the operation is cumbersome. Content of the Utility Model

[0004] The utility model provides a somatic embryo culture device for Rhododendron lapponicum, which solves the problem that when the existing cell culture incubator in the related technology is in use, multiple culture dishes are generally placed, so when it is necessary to observe the internal culture dishes, they need to be taken out one by one for observation, which is inconvenient to use, wastes the observation time, and the operation is cumbersome.

[0005] The technical solution of the utility model is as follows:

[0006] A somatic embryo culture device for Rhododendron lapponicum, comprising:

[0007] A cell culture incubator body, a sealed box door is rotatably installed on the front wall of the cell culture incubator body, and an observation window is installed on the sealed box door;

[0008] A mounting plate, a turntable is rotatably installed in the center of the mounting plate, a plurality of culture dish placement seats are symmetrically and rotatably installed on the top wall of the turntable, a bottom cover is fixed to the bottom wall of the mounting plate, a Geneva mechanism for driving the turntable to rotate is installed in the bottom cover, and a rotating assembly for driving the culture dish placement seats to rotate is installed in the turntable;

[0009] A sealing assembly, the sealing assembly is fixed on the top wall of the inner cavity of the cell culture incubator body.

[0010] Preferably, the Geneva mechanism includes a driving dial and a driven Geneva wheel. A sleeve is fixed in the center of the driven Geneva wheel, the top end of the sleeve is fixed to the center of the bottom wall of the turntable, and the bottom end of the sleeve is rotatably connected to the top wall of the bottom cover;

[0011] A dial rod that cooperates with the driven grooved wheel is fixed to the top wall of the active dial, and the active dial is rotatably installed on the top wall of the bottom cover;

[0012] A second motor is fixed to one side of the bottom wall of the bottom cover, and the power shaft on the top wall of the second motor passes through the bottom wall of the bottom cover through a bearing and is fixedly connected to the central position of the bottom of the active dial.

[0013] Preferably, the number of radial grooves on the driven grooved wheel is the same as the number of culture dish placement seats.

[0014] Preferably, the rotating assembly includes a driving gear, and the driving gear is rotatably installed in the center of the inner cavity of the turntable;

[0015] An external toothed ring is fixed to one end of the culture dish placement seat located in the inner cavity of the turntable, and the external toothed rings are all meshed with the driving gear;

[0016] A first motor is fixed to the center of the bottom wall of the bottom cover, and the power shaft of the first motor passes through the bottom walls of the bottom cover and the turntable through a bearing and is fixedly connected to the central position of the bottom of the driving gear. The shaft sleeve is movably sleeved outside the power shaft of the first motor.

[0017] Preferably, both the first motor and the second motor are servo motors or stepper motors.

[0018] Preferably, the sealing assembly includes a cylinder, the cylinder is fixed to the top wall of the inner cavity of the cell culture box, and a connecting plate is fixed to the movable end at the bottom of the cylinder. Rotating plates are symmetrically rotatably installed on the bottom wall of the connecting plate.

[0019] Preferably, the rotating plates correspond to the positions of the culture dish placement seats, and a sealing gasket is detachably fixed to the bottom wall of the rotating plates.

[0020] Preferably, a handle is fixed to the front wall of the sealing box door.

[0021] The beneficial effects of the present utility model are as follows:

[0022] 1. In the present utility model, the grooved wheel mechanism is provided to drive the turntable to rotate, so as to rotate the culture dish located inside to the outside, which is convenient for observation and the operation is simple and convenient.

[0023] 2. In the present utility model, the rotating assembly provided can drive the culture dish placement seat to rotate, so as to realize the shaking culture of Rhododendron lapponicum somatic cells in the culture dish, and the sealing assembly provided is used to block the top of the culture dish, so as to avoid the liquid spilling out during the rotation of the culture dish. Description of the Drawings

[0024] The present utility model will be further described in detail below in conjunction with the drawings and specific embodiments.

[0025] Figure 1 This is a three-dimensional view of the external structure of the present utility model;

[0026] Figure 2 This is a three-dimensional view of the external structure of the present utility model in the open state of the sealed box door;

[0027] Figure 3 This is a three-dimensional view of the bottom structure of the mounting plate of the present utility model;

[0028] Figure 4 This is a three-dimensional view of the Geneva wheel mechanism structure of the present utility model;

[0029] Figure 5 This is a three-dimensional view of the internal structure of the turntable of the present utility model;

[0030] Figure 6 This is a three-dimensional view of the sealing component structure of the present utility model.

[0031] In the figure: 1. Cell culture box body; 2. Sealed box door; 3. Observation window; 4. Mounting plate; 5. Turntable; 6. Petri dish placement seat; 7. Bottom cover; 8. Driving dial; 9. Driven Geneva wheel; 10. Dial rod; 11. Sleeve; 12. First motor; 13. Second motor; 14. Driving gear; 15. External toothed ring; 16. Cylinder; 17. Connecting plate; 18. Rotating plate; 19. Handle; 20. Circular plate. Specific embodiments

[0032] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts fall within the scope of protection of the present utility model.

[0033] Embodiment 1

[0034] As Figures 1 to 2 shown, this embodiment proposes:

[0035] A somatic embryo culture device for Rhododendron lapponicum, comprising:

[0036] A cell culture box body 1, a sealed box door 2 is rotatably installed on the front wall of the cell culture box body 1, and an observation window 3 is installed on the sealed box door 2;

[0037] A mounting plate 4, a turntable 5 is rotatably installed in the center of the mounting plate 4, a plurality of petri dish placement seats 6 are symmetrically and rotatably installed on the top wall of the turntable 5, a bottom cover 7 is fixed to the bottom wall of the mounting plate 4, a Geneva wheel mechanism for driving the turntable 5 to rotate is installed in the bottom cover 7, and a rotating assembly for driving the petri dish placement seats 6 to rotate is installed in the turntable 5;

[0038] The sealing component is fixed on the top wall of the inner cavity of the cell incubator body 1.

[0039] A handle 19 is fixed on the front wall of the sealed chamber door 2.

[0040] In this embodiment, the cell incubator body 1 set is a conventional plant cell culture device, which can provide the required ecological environment for the somatic cell culture of Rhododendron lapponicum, such as specific temperature, humidity, carbon dioxide content, etc. The sealed chamber door 2 set can facilitate the removal or insertion of the culture dish from the cell incubator body 1. The observation window 3 set can facilitate the direct observation of the development state of the somatic embryos of Rhododendron lapponicum. The culture dish placement seat 6 set is used to place the culture dish for culturing the somatic cells of Rhododendron lapponicum. The groove set on the culture dish placement seat 6 coincides with the bottom of the culture dish. At the same time, a rubber pad is provided on the inner wall of the groove, which can stably snap the culture dish into the groove of the culture dish placement seat 6. The grooved wheel mechanism set is used to drive the turntable 5 to rotate, so as to rotate the culture dish on the inner side to the outer side for convenient observation. The rotating component set can drive the culture dish placement seat 6 to rotate, so as to realize the shaking culture of the somatic cells of Rhododendron lapponicum in the culture dish. The sealing component set is used to block the top of the culture dish, so as to prevent the liquid from spilling out during the rotation of the culture dish. The handle 19 set can facilitate the opening and closing of the sealed chamber door 2.

[0041] Embodiment 2

[0042] As Figures 3 to 6 shown, based on the same concept as the above Embodiment 1, this embodiment also proposes:

[0043] The grooved wheel mechanism includes a driving dial 8 and a driven grooved wheel 9. A sleeve 11 is fixed in the center of the driven grooved wheel 9. The top of the sleeve 11 is fixed in the center of the bottom wall of the turntable 5. The bottom of the sleeve 11 is rotatably connected to the top wall of the bottom cover 7;

[0044] A dial rod 10 that cooperates with the driven grooved wheel 9 is fixed on the top wall of the driving dial 8. The driving dial 8 is rotatably installed on the top wall of the bottom cover 7;

[0045] A second motor 13 is fixed on one side of the bottom wall of the bottom cover 7. The power shaft on the top wall of the second motor 13 passes through the bottom wall of the bottom cover 7 through a bearing and is fixedly connected to the bottom center position of the driving dial 8.

[0046] The number of radial grooves on the driven grooved wheel 9 is the same as the number of culture dish placement seats 6.

[0047] In this embodiment, the Geneva mechanism is provided to drive the turntable 5 to rotate, so as to rotate the culture dish located inside to the outside for convenient observation. When the Geneva mechanism works, the second motor 13 drives the driving dial 8 to rotate. The driving dial 8 cooperates with the dial rod 10 to drive the driven Geneva wheel 9 to rotate a certain angle each time, driving the culture dish to rotate to the front side in turn, facilitating the observation of the Rhododendron lapponicum somatic cells cultured in the culture dish.

[0048] The rotating assembly includes a driving gear 14, and the driving gear 14 is rotatably installed at the center of the inner cavity of the turntable 5;

[0049] Outer tooth rings 15 are fixedly arranged at one end of the culture dish placement seat 6 located in the inner cavity of the turntable 5, and the outer tooth rings 15 are all meshed with the driving gear 14;

[0050] A first motor 12 is fixedly arranged at the center of the bottom wall of the bottom cover 7. The power shaft of the first motor 12 passes through the bottom walls of the bottom cover 7 and the turntable 5 through bearings and is fixedly connected to the bottom center position of the driving gear 14. The sleeve 11 is movably sleeved outside the power shaft of the first motor 12.

[0051] Both the first motor 12 and the second motor 13 are servo motors or stepper motors.

[0052] The sealing assembly includes a cylinder 16. The cylinder 16 is fixedly arranged on the top wall of the inner cavity of the cell culture box body 1. The movable end at the bottom of the cylinder 16 is fixedly provided with a connecting plate 17, and rotating plates 18 are symmetrically and rotatably installed on the bottom wall of the connecting plate 17.

[0053] The rotating plates 18 correspond to the positions of the culture dish placement seats 6, and a sealing gasket is detachably fixed to the bottom wall of the rotating plates 18.

[0054] The provided rotating assembly can drive the culture dish placement seat 6 to rotate, so as to realize the shaking culture of the Rhododendron lapponicum somatic cells in the culture dish. When the rotating assembly works, the first motor 12 drives the driving gear 14 to rotate, and the driving gear 14 drives the culture dish placement seat 6 to rotate through the outer tooth ring 15, thereby driving the culture dish to rotate. The provided sealing assembly is used to block the top wall of the culture dish during the rotation of the culture dish to prevent the liquid from spilling out during the rotation. When using the sealing assembly, control the cylinder 16 to extend until the rotating plate 18 fits on the top of the culture dish.

[0055] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A device for culturing somatic embryos of Rhododendron albifrons, characterized in that: include: A cell culture box body (1), wherein a sealed box door (2) is rotatably mounted on the front wall of the cell culture box body (1), and an observation window (3) is mounted on the sealed box door (2); A mounting plate (4), a rotating disc (5) being rotatably mounted in the center of the mounting plate (4), a plurality of culture dish placement seats (6) being symmetrically rotatably mounted on the top wall of the rotating disc (5), a bottom cover (7) being fixed to the bottom wall of the mounting plate (4), a grooved wheel mechanism for driving the rotating disc (5) to rotate being mounted in the bottom cover (7), and a rotating assembly for driving the culture dish placement seats (6) to rotate being mounted in the rotating disc (5); A sealing component, wherein the sealing component is fixed on the top wall of the inner cavity of the cell culture chamber body (1).

2. The device for culturing somatic embryos of Rhododendron albifrons according to claim 1, characterized in that: The sheave mechanism comprises an active dial (8) and a driven sheave (9), a shaft sleeve (11) being fixed at the center of the driven sheave (9), a top end of the shaft sleeve (11) being fixed at the center of the bottom wall of the rotating disk (5), and a bottom end of the shaft sleeve (11) being rotatably connected to the top wall of the bottom cover (7); A lever (10) cooperating with the driven groove wheel (9) is fixed on the top wall of the active dial (8), and the active dial (8) is rotatably mounted on the top wall of the bottom cover (7); A second motor (13) is fixed to one side of the bottom wall of the bottom cover (7), and a power shaft on the top wall of the second motor (13) passes through the bottom wall of the bottom cover (7) through a bearing and is fixedly connected to the bottom axis position of the active dial (8).

3. The device for culturing somatic embryos of Rhododendron albifrons according to claim 2, characterized in that: The number of radial grooves on the driven groove wheel (9) is the same as the number of the culture dish placement seat (6).

4. The device for culturing somatic embryos of Rhododendron albifrons according to claim 2, characterized in that: The rotating assembly comprises a driving gear (14), and the driving gear (14) is rotatably mounted in the center of the inner cavity of the rotating disk (5); An outer toothed ring (15) is fixed to one end of the culture dish placement seat (6) located in the inner cavity of the turntable (5), and the outer toothed ring (15) is meshed with the driving gear (14); A No. 1 motor (12) is fixed at the center of the bottom wall of the bottom cover (7); a power shaft of the No. 1 motor (12) passes through the bottom cover (7) and the bottom wall of the turntable (5) via a bearing and is fixedly connected to the bottom axis position of the driving gear (14); and the shaft sleeve (11) is movably sleeved outside the power shaft of the No. 1 motor (12).

5. The device for culturing somatic embryos of Rhododendron albifrons according to claim 4, characterized in that: The first motor (12) and the second motor (13) are both servo motors or stepper motors.

6. The device for culturing somatic embryos of Rhododendron albifrons according to claim 1, characterized in that: The sealing assembly comprises a cylinder (16), the cylinder (16) being fixed on the top wall of the inner cavity of the cell culture box body (1), a connecting plate (17) being fixed to the movable end at the bottom of the cylinder (16), and a rotating plate (18) being symmetrically rotatably mounted on the bottom wall of the connecting plate (17).

7. The device for culturing somatic embryos of Rhododendron albifrons according to claim 6, characterized in that: The rotating plate (18) corresponds to the position of the culture dish placement seat (6), and a sealing gasket is detachably fixed on the bottom wall of the rotating plate (18).

8. The device for culturing somatic embryos of Rhododendron albifrons according to claim 1, characterized in that: A handle (19) is fixed to the front wall of the sealed box door (2).

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