Half-dry transfer electrophoresis apparatus

Abstract

The utility model discloses a semi-dry transfer electrophoresis apparatus, which relates to the technical field of electrophoresis devices, and comprises an upper cover component and a lower groove component, the upper cover component is used for connecting a negative electrode of a power supply, the lower groove component is used for connecting a positive electrode of the power supply, a stainless steel plate is arranged on the lower end face of the upper cover component, and a platinum-plated titanium plate is arranged on the upper end face of the lower groove component. The upper end of the platinum-plated titanium plate is used for placing a transfer printing unit, the upper cover assembly can be buckled in the lower groove assembly, and the stainless steel plate and the platinum-plated titanium plate are aligned up and down and press the transfer printing unit. The transfer printing device is long in service life, and the transfer printing efficiency and the transfer printing effect can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of electrophoresis devices, in particular to a semi-dry transfer electrophoresis apparatus. Background Art

[0002] An electrophoresis device consists of a power supply, electrodes, and an electrophoresis apparatus. The principle is to utilize the property that charged particles have different moving speeds and directions due to different charged properties to achieve the purpose of separation. Its intended use is for the separation of components in human samples. For an electrophoresis apparatus used for transfer, it is a device used for transfer electrophoresis of a small amount of nucleic acid and protein samples. During the transfer process, a gel, filter paper, transfer membrane, etc. need to be placed inside for transfer, and an electric field is used to transfer the protein in the gel to the transfer membrane.

[0003] However, the existing electrophoresis apparatus uses a carbon plate as the conductive material for connecting the positive electrode. However, during the transfer process, since the carbon plate needs to be in contact with the buffer solution for a long time, it is extremely easy to produce liquid leakage and foaming phenomena, and it is easily corroded by the buffer solution, affecting the overall service life. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a semi-dry transfer electrophoresis apparatus to solve the problems existing in the above-mentioned prior art, extend the service life, and improve the transfer efficiency and transfer effect.

[0005] To achieve the above purpose, the utility model provides the following solution:

[0006] The utility model provides a semi-dry transfer electrophoresis apparatus, which includes an upper cover assembly and a lower tank assembly. The upper cover assembly is used to connect to the negative electrode of the power supply, and the lower tank assembly is used to connect to the positive electrode of the power supply. A stainless steel plate is provided on the lower end surface of the upper cover assembly, and a platinum-plated titanium plate is provided on the upper end surface of the lower tank assembly. The upper end of the platinum-plated titanium plate is used to place the transfer unit. The upper cover assembly can be buckled into the lower tank assembly, and the stainless steel plate and the platinum-plated titanium plate are vertically aligned and press the transfer unit.

[0007] Preferably, the upper cover assembly includes a handle, a top cover, and a top sealing plate. The handle can be detachably installed on the upper end of the top cover, and the top sealing plate can be detachably installed on the lower end of the top cover, and the outer edge of the top sealing plate is flush with the outer edge of the top cover.

[0008] Preferably, the two connecting ends of the handle are installed on the upper end surface of the top cover through bolts.

[0009] Preferably, the four corners of the top cover are respectively connected to the four corners of the top sealing plate through bolts.

[0010] Preferably, the negative electrode of the power supply passes through the top cover and the top sealing plate in sequence and is connected to the stainless steel plate.

[0011] Preferably, the lower tank assembly includes an outer tank body and a bottom sealing plate. The bottom sealing plate is detachably mounted on the inner wall of the outer tank body, and the upper cover assembly can be buckled into the outer tank body.

[0012] Preferably, the four corners of the bottom sealing plate are bolted to the four corners of the inner bottom surface of the outer tank body.

[0013] Preferably, the positive electrode of the power supply passes through the side wall of the outer tank body and the bottom sealing plate and is connected to the platinum-plated titanium plate.

[0014] Preferably, an insulating gasket is further included. The insulating gasket is a rectangular frame. The insulating gasket is placed between the stainless steel plate and the platinum-plated titanium plate. The outer edge of the insulating gasket extends close to the inner side wall of the lower tank assembly, and the outer edge of the insulating gasket extends out of the outer edges of the stainless steel plate and the platinum-plated titanium plate. The inner edge of the insulating gasket extends between the stainless steel plate and the platinum-plated titanium plate.

[0015] Preferably, the outer edge of the lower end surface of the upper cover assembly is bolted to the outer edge of the stainless steel plate; the outer edge of the platinum-plated titanium plate is bolted to the outer edge of the upper end surface of the lower tank assembly.

[0016] The utility model has achieved the following technical effects compared with the prior art:

[0017] The semi-dry transfer electrophoresis apparatus provided by the utility model, the upper cover assembly is used to connect the negative electrode of the power supply, the lower tank assembly is used to connect the positive electrode of the power supply. The lower end surface of the upper cover assembly is provided with a stainless steel plate, the upper end surface of the lower tank assembly is provided with a platinum-plated titanium plate. The upper end of the platinum-plated titanium plate is used to place the transfer unit. The upper cover assembly can be buckled into the lower tank assembly, and the stainless steel plate and the platinum-plated titanium plate are vertically aligned and press the transfer unit. By energizing the stainless steel plate and the platinum-plated titanium plate, the transfer process is realized. At the same time, since the platinum-plated titanium plate is used to replace the traditional titanium plate, it has various characteristics such as electrolytic corrosion resistance, high temperature resistance, and good electrical conductivity, prolongs the service life, and is convenient for cleaning, maintenance and replacement, improving the transfer efficiency and transfer effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0019] Figure 1 It is a schematic structural diagram of the semi-dry transfer electrophoresis apparatus in the present invention;

[0020] Figure 2 is Figure 1 Schematic structural view of the semi-dry transfer electrophoresis apparatus in the present invention when partial side walls are omitted;

[0021] Figure 3 is Figure 2 Schematic structural view of the semi-dry transfer electrophoresis apparatus in the present invention when the upper cover assembly is omitted;

[0022] Figure 4 Top view of the semi-dry transfer electrophoresis apparatus of the present invention;

[0023] Figure 5 is Figure 4 A - A cross-sectional view of

[0024] In the figure: 1 - top cover, 2 - top sealing plate, 3 - stainless steel plate, 4 - insulating gasket, 5 - platinum-plated titanium plate, 6 - bottom sealing plate, 7 - outer tank body, 8 - handle, 9 - negative power supply, 10 - positive power supply, 11 - buckling groove. Specific embodiments

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

[0026] The purpose of the present invention is to provide a semi-dry transfer electrophoresis apparatus to solve the problems existing in the prior art, extend the service life, and improve the transfer efficiency and transfer effect.

[0027] To make the above objects, features, and advantages of the present invention more obvious and understandable, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0028] Such as Figures 1 - 5As shown in the figure, this embodiment provides a semi-dry transfer electrophoresis apparatus, which is applicable to electrophoresis tests carried out in medical institutions and laboratories. It includes an upper cover assembly and a lower tank assembly. Both the upper cover assembly and the lower tank assembly are made of corrosion-resistant insulating materials. The upper cover assembly is used to connect to the negative electrode 9 of the power supply, and the lower tank assembly is used to connect to the positive electrode 10 of the power supply. The lower end surface of the upper cover assembly is provided with a stainless steel plate 3 serving as the cathode. The surface of the stainless steel plate 3 is smooth and flat. The upper end surface of the lower tank assembly is provided with a platinum-plated titanium plate 5 serving as the anode. The stainless steel plate 3 and the platinum-plated titanium plate 5 have the same size, and their installation positions can be exchanged. The upper end of the platinum-plated titanium plate 5 is used to place the transfer unit, that is, the transfer membrane gasket, transfer membrane, gel, etc. The upper cover assembly can be buckled into the lower tank assembly, so that the stainless steel plate 3 and the platinum-plated titanium plate 5 are aligned vertically and press the transfer unit. By energizing the stainless steel plate 3 and the platinum-plated titanium plate 5, the transfer process is realized. At the same time, since the platinum-plated titanium plate 5 is used to replace the traditional titanium plate, it has various characteristics such as resistance to electrolytic corrosion, high temperature resistance, and good electrical conductivity, which prolongs the service life, and is also convenient for cleaning, maintenance and replacement, improving the transfer efficiency and transfer effect.

[0029] Specifically, the upper cover assembly includes a handle 8, a top cover 1 and a top sealing plate 2. The handle 8 is detachably installed on the upper end of the top cover 1, which is convenient for opening and closing the top cover 1 when placing and taking out the transfer unit. The top sealing plate 2 is detachably installed on the lower end of the top cover 1, and the outer edge of the top sealing plate 2 is flush with the outer edge of the top cover 1.

[0030] The two connection ends of the handle 8 are installed on the upper end surface of the top cover 1 through bolts, improving the connection stability.

[0031] The four corners of the top cover 1 are respectively connected to the four corners of the top sealing plate 2 through bolts. While realizing the reliable connection between the top cover 1 and the top sealing plate 2, it also avoids affecting the installation of the stainless steel plate 3 and the progress of the transfer operation.

[0032] The negative electrode 9 of the power supply passes through the top cover 1 and the top sealing plate 2 in sequence and is connected to the stainless steel plate 3 to connect the stainless steel plate 3 to the negative electrode 9 of the power supply.

[0033] The lower tank assembly includes an outer tank body 7 and a bottom sealing plate 6. The bottom sealing plate 6 is detachably installed on the inner wall of the outer tank body 7, and the upper cover assembly can be buckled into the outer tank body 7. The four side walls of the outer tank body 7 are arranged in pairs opposite to each other, and two opposite side walls are provided with buckles 11 at the upper ends. The buckles 11 can be used for hand-held, which is convenient for taking the whole semi-dry transfer electrophoresis apparatus.

[0034] The four corners of the bottom sealing plate 6 are connected to the four corners of the inner bottom surface of the outer tank body 7 through bolts. While realizing the reliable connection between the bottom sealing plate 6 and the outer tank body 7, it also avoids affecting the installation of the platinum-plated titanium plate 5 and the progress of the transfer operation.

[0035] The positive electrode 10 of the power supply passes through the side wall and the bottom sealing plate 6 of the outer tank body 7 and is connected to the platinum-plated titanium plate 5 to connect the platinum-plated titanium plate 5 to the positive electrode 10 of the power supply.

[0036] This embodiment further includes an insulating gasket 4. The insulating gasket 4 is a rectangular frame, that is, the middle part of the insulating gasket 4 is hollow. The insulating gasket 4 is placed between the stainless steel plate 3 and the platinum-plated titanium plate 5. The outer edge of the insulating gasket 4 extends to be close to the inner side wall of the lower tank assembly, and the outer edge of the insulating gasket 4 extends to extend beyond the outer edges of the stainless steel plate 3 and the platinum-plated titanium plate 5. The inner edge of the insulating gasket 4 extends to be located between the stainless steel plate 3 and the platinum-plated titanium plate 5. Thus, the area surrounded by the inner edge of the insulating gasket 4, the lower end face of the stainless steel plate 3, and the upper end face of the platinum-plated titanium plate 5 forms a transfer working area for placing the transfer unit. The insulating gasket 4 is preferably made of PE material.

[0037] The outer edge of the lower end face of the upper cover assembly is bolted to the outer edge of the stainless steel plate 3; the outer edge of the platinum-plated titanium plate 5 is bolted to the outer edge of the upper end face of the lower tank assembly, which is convenient for installation and disassembly.

[0038] The normal working conditions of the semi-dry transfer electrophoresis apparatus in this embodiment are: ambient temperature 0°C to 40°C; relative humidity ≤80%; no strong vibration around; the experimental table should be flat.

[0039] The usage method of the semi-dry transfer electrophoresis apparatus in this embodiment is as follows:

[0040] S1. Prepare the gel, filter paper, and transfer membrane.

[0041] S2. Cut the filter paper and transfer membrane with scissors, note that it should be the same size as or slightly smaller than the gel cut, and wear gloves during operation.

[0042] S3. Immerse the filter paper and transfer membrane fully in the electrotransfer buffer.

[0043] S4. Stack and combine the gel, filter paper, and transfer membrane to form a transfer unit. To ensure uniform transfer, carefully roll a stirring rod or use a transfer roller on the surface of each layer in the transfer unit to remove air bubbles, and do not apply too much pressure to avoid damaging the transfer membrane and gel.

[0044] S5. Place the insulating gasket 4 into the lower tank assembly.

[0045] S6. Place the transfer unit at the hollow position of the insulating gasket 4, and cover the upper cover assembly, with the stainless steel plate 3 on top and the alloy-plated titanium plate below, so that the cathode is on top and the anode is below.

[0046] S7. Connect the electrophoresis wire between the semi-dry transfer electrophoresis apparatus and the power supply.

[0047] S8. Turn on the power switch of the semi-dry transfer electrophoresis instrument and set the electrophoresis parameters (it is recommended to use a constant current generally, 1 - 3 mA / cm 2 ), start electrophoresis. During the operation, do not collide with the upper cover assembly to avoid disturbing the alignment of the stacked layer in the transfer unit and making the results inaccurate;

[0048] S9. After electrophoresis is completed, first turn off the power switch, then pull out the electrophoresis wire, and take out the transfer unit.

[0049] In the present utility model, specific examples are used to elaborate on the principle and implementation manner of the present utility model. The description of the above embodiments is only used to help understand the method of the present utility model and its core idea; at the same time, for those of ordinary skill in the art, according to the idea of the present utility model, there will be changes in the specific implementation manner and application scope. In summary, the content of this specification should not be construed as a limitation to the present utility model.

Claims

1. A semi-dry transfer electrophoresis apparatus, characterized in that: It includes an upper cover assembly and a lower tank assembly, the upper cover assembly is used to connect to the negative pole of the power supply, the lower tank assembly is used to connect to the positive pole of the power supply, the lower end surface of the upper cover assembly is provided with a stainless steel plate, the upper end surface of the lower tank assembly is provided with a platinum-plated titanium plate, the upper end of the platinum-plated titanium plate is used to place the transfer unit, the upper cover assembly can be buckled into the lower tank assembly, and the stainless steel plate and the platinum-plated titanium plate are aligned up and down and press the transfer unit.

2. The semi-dry transfer electrophoresis apparatus according to claim 1, characterized in that: The upper cover assembly includes a handle, a top cover and a top sealing plate, wherein the handle is detachably mounted on the upper end of the top cover, the top sealing plate is detachably mounted on the lower end of the top cover, and the outer edge of the top sealing plate is flush with the outer edge of the top cover.

3. The semi-dry transfer electrophoresis apparatus according to claim 2, characterized in that: The two connecting ends of the handle are mounted on the upper end surface of the top cover by bolts.

4. The semi-dry transfer electrophoresis apparatus according to claim 2, characterized in that: The four corners of the top cover are respectively connected to the four corners of the top sealing plate by bolts.

5. The semi-dry transfer electrophoresis apparatus according to claim 2, characterized in that: The negative electrode of the power supply passes through the top cover and the top sealing plate in sequence and is connected to the stainless steel plate.

6. The semi-dry transfer electrophoresis apparatus according to claim 1, characterized in that: The lower tank assembly includes an outer tank body and a bottom sealing plate. The bottom sealing plate can be detachably mounted on the inner wall of the outer tank body, and the upper cover assembly can be buckled into the outer tank body.

7. The semi-dry transfer electrophoresis apparatus according to claim 6, characterized in that: The four corners of the bottom sealing plate are connected to the four corners of the inner bottom surface of the outer tank body by bolts.

8. The semi-dry transfer electrophoresis apparatus according to claim 6, characterized in that: The positive electrode of the power supply passes through the side wall of the outer tank body and the bottom sealing plate and is connected to the platinum-plated titanium plate.

9. The semi-dry transfer electrophoresis apparatus according to claim 1, characterized in that: It also includes an insulating gasket, which is a rectangular frame. The insulating gasket is placed between the stainless steel plate and the platinum-plated titanium plate. The outer edge of the insulating gasket extends to the inner wall close to the lower tank assembly, and the outer edge of the insulating gasket extends to extend beyond the outer edge of the stainless steel plate and the outer edge of the platinum-plated titanium plate, and the inner edge of the insulating gasket extends to be located between the stainless steel plate and the platinum-plated titanium plate.

10. The semi-dry transfer electrophoresis apparatus according to claim 1, characterized in that: The outer edge of the lower end surface of the upper cover assembly is connected to the outer edge of the stainless steel plate via bolts; the outer edge of the platinum-plated titanium plate is connected to the outer edge of the upper end surface of the lower tank assembly via bolts.

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