Electrophoretic mask device
By designing an electrophoretic mask device, using the structure of the mask plate and the mask strip, uniform addition of reactants was achieved, which solved the problem of uneven addition of reactants in existing electrophoretic experiments, and improved the adequacy of the reaction and the standardization level of the test.
Patent Information
- Application Number
- CN202421706166.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-18
- Publication Date
- 2025-06-24
- Estimated Expiration
- 2034-07-18
AI Technical Summary
In existing electrophoresis experiments, manual coating of reactants leads to uneven addition of reactants and insufficient reaction.
An electrophoretic mask device is designed. By opening an additive hole on the mask plate and communicating with the opening on the mask strip, the liquid falls into the mask strip through the addition hole, and the liquid spreads evenly along the mask strip under the action of surface tension to achieve uniform addition of reactants.
The uniform addition of reactants is achieved, the adequacy of the reaction is ensured, the test operation is simplified, and the standardization level of the test is improved.
Smart Images
Figure CN223022020U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of mask devices, in particular to an electrophoresis mask device. Background Art
[0002] Electrophoresis experiment is an experimental method for separating substances. This method separates atoms, molecules, cells and other molecules in a mixture through the action of an electric field, so as to obtain a sample with high purity.
[0003] Electrophoresis experiments can be used to separate biological macromolecules such as DNA, RNA, and proteins, and can also be used to separate organic substances such as drugs and cosmetics. In the biological field, electrophoresis experiments are often used to study microscopic structures such as genes, human cells, and bacteria, so as to find new drugs or treatment plans.
[0004] Electrophoresis experiment is one of the important separation technologies in the fields of modern biology and chemistry, and its application scope is wide. With the development of fields such as molecular biology and biotechnology, electrophoresis experiments play an increasingly important role in research, and its technology has been continuously improved and developed.
[0005] In the existing electrophoresis experiments, manual coating is mainly used. This method is not convenient for the standardized operation of the experiment. When adding reactants, it is easy to cause uneven addition of reactants and insufficient reaction. Summary of the Utility Model
[0006] In view of this, to solve the technical problem that in the existing electrophoresis experiment, when manually coating reactants, it is easy to cause uneven addition of reactants, resulting in insufficient reaction, the utility model provides an electrophoresis mask device, which opens adding holes on a mask plate and communicates with the openings on a mask strip. The liquid falls into the mask strip through the adding holes, and under the action of surface tension, the liquid uniformly diffuses along the mask strip, so as to achieve the purpose of uniformly adding reactants.
[0007] To achieve the above object, the utility model provides the following technical solutions:
[0008] An electrophoresis mask device, comprising:
[0009] A mask plate, on which a plurality of adding holes are opened;
[0010] A mask frame, which is arranged on the side with a smaller opening of the adding hole and plays a supporting role;
[0011] A mask strip, which is a guide groove, and a through hole for communicating with the smaller opening end of the adding hole is opened on the back of its opening;
[0012] A height difference is formed between the mask frame and the mask strip, and the height of the mask strip is lower than the height of the mask frame;
[0013] Liquid enters the guiding groove from the adding hole and diffuses evenly within the guiding groove.
[0014] Preferably, it further includes:
[0015] A positioning device, which is arranged at the bottom of the mask plate and is used to accurately place the mask plate above the film.
[0016] Preferably, it further includes:
[0017] A handle, which is arranged on the upper part of the mask plate.
[0018] Preferably, the adding hole is at least one of a conical hole, a circular hole, a square hole and an irregular hole.
[0019] Preferably, the adding hole is a conical hole.
[0020] Preferably, the height difference between the mask frame and the mask strip is 0.05 mm to 5 mm.
[0021] Preferably, the height difference between the mask frame and the mask strip is 0.1 - 2 mm.
[0022] Preferably, the material of the mask device is a transparent material.
[0023] The utility model has the following beneficial effects compared with the prior art:
[0024] The electrophoresis mask device provided by the utility model opens an adding hole on the mask plate and communicates with the opening on the mask strip. Liquid (reactant) falls into the mask strip through the adding hole. Under the action of surface tension, since the mask strip is in the form of a guiding groove, the liquid diffuses evenly along the mask strip, so as to achieve the purpose of uniformly adding reactants.
[0025] Since the utility model uses an adding hole to add reactants, the diameter and depth of the adding hole determine the volume of liquid that can be stored, and the diameter and depth of the adding hole can be set according to the actual amount of reactants to be added. The volume formed by the width of the mask strip and the height difference from the mask frame is adapted to this, thus ingeniously solving the problem of controlling the addition amount of reactants.
[0026] The bottom of the mask plate of the utility model is provided with a positioning device, so that the user can conveniently and accurately place the mask plate above the film. No handle is arranged on the mask plate, which can prevent the user from touching the film when taking the mask plate. At the same time, the position distribution of the adding holes on the mask plate is provided with a first hole position anti-fooling setting, which is more user-friendly and can prevent wrong addition.
[0027] In summary, when using the device of the present utility model to uniformly add reactants on the film lane, the reactant addition method is convenient, the reactant coating is uniform, and the reaction is sufficient. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 is an exploded view of the present utility model;
[0029] Figure 2 is a three-dimensional view of the present utility model;
[0030] Figure 3 is the front view of the present utility model;
[0031] Figure 4 is the rear view of the present utility model;
[0032] In the figure, 1 is a mask plate; 11 is an addition hole; 12 is a positioning device; 13 is a handle; 2 is a mask frame; 3 is a mask strip; 31 is a through hole. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0033] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model; obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.
[0034] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "top / bottom end", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.
[0035] In the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "provided with", "sheathed / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.
[0036] Such as Figures 1-4As shown, the present utility model provides an electrophoresis mask device, comprising:
[0037] A mask plate 1, on which a plurality of adding holes 11 are provided. The adding holes 11 have one end with a large opening and one end with a small opening. The adding holes 11 are preferably at least one of a tapered hole, a circular hole, a square hole, and an irregular hole, and more preferably a tapered hole. Its diameter and depth can be determined according to the amount of reactants to be added; the shape and size of the adding holes 11 can be selected according to actual needs, and no special requirements are made for this, as long as the added liquid can uniformly diffuse through the through holes in the following mask strips.
[0038] A mask frame 2, which is arranged on the side with the small opening of the adding hole 11, plays a supporting role, and forms a height difference with the mask strip, as Figure 2 、 4 shown. It shows that the mask frame 2 is preferably rectangular and is arranged on the left and right sides of the mask plate 1.
[0039] It should be noted that the shape of the mask frame 2 is not limited to a rectangle, not limited to the left, right, front, and back sides of the mask plate, and can also be set as a column shape and placed in the middle position of the mask plate 1;
[0040] A mask strip 3, which is a guide groove, and a through hole 31 for communicating with the small opening end of the adding hole 11 is provided on the back of its opening;
[0041] The height of the mask strip 3 is lower than the height of the mask frame 2 to form a space for accommodating the film;
[0042] The liquid enters the guide groove from the adding hole 11 and uniformly diffuses in the guide groove.
[0043] In the present utility model, it further comprises:
[0044] A positioning device 12, which is arranged at the bottom of the mask plate 1 and is used to accurately place the mask plate 1 above the film. Among them, the positioning device 12 can be integrally formed with the mask plate 1, and its shape is as Figure 1 shown.
[0045] In the present utility model, it further comprises:
[0046] A handle 13, which is arranged on the upper part of the mask plate 1. It is preferably integrally formed with the mask plate 1, and its shape is as Figure 1 shown.
[0047] In the present utility model, the adding hole is at least one of a tapered hole, a circular hole, a square hole, and an irregular hole.
[0048] In the present utility model, the adding hole is a tapered hole.
[0049] In the present invention, the height difference between the mask frame 2 and the mask strip 3 is preferably 0.05 mm to 5 mm, more preferably 0.1-2 mm.
[0050] In the present invention, the material of the mask device is transparent acrylic material, but is not limited to acrylic material, and other materials that have no effect on the experiment can be used.
[0051] The utility model has a first hole position foolproof setting for the position distribution of the adding hole 11 on the mask plate 1, which is more humane and prevents adding errors. Figure 3 As shown, every certain number of wells are set at another horizontal line to prevent the user from visual fatigue when adding samples, resulting in wrong addition or omission.
[0052] The following are some examples:
[0053] The mask strips can be made with the same width and height and arranged at equal intervals. For example, if there are 12 horizontal mask strips, a patient needs 3 mask strips for 3 tests, then the next patient needs strips 4 to 6, and the next patient needs strips 7 to 9. In order to prevent the user from clicking the wrong sample, a hole needs to be opened on another horizontal line for marking.
[0054] In the mask device provided by the present invention, the mask plate 1 can be hydrophobic or hydrophilic, and the selection can be made according to actual needs.
[0055] The number of channels formed by the mask strip 3 can be selected according to actual needs, and its width can be narrower, equal to or wider than the width of the swimming lane. The length of the channel formed by the mask strip 3 can be adjusted according to the length of the swimming lane, and the middle can also be partitioned. The carrier of the swimming lane can be different media such as agarose gel, PAGE gel, cellulose acetate, etc.
[0056] The method of using the utility model is as follows:
[0057] Assemble the mask plate 1, the mask frame 2 and the mask strip 3, and place the film under the mask plate 1 and within the mask frame 2; at this time, the mask strip 3 is precisely distributed below the mask plate 1 and above the film to form a space; the adding hole 11 is aligned with the hole position of the through hole 31 on the mask strip 3, and when adding reactants, the liquid falls into this space through the conical hole, and the liquid is evenly diffused along the mask strip 3 under the action of surface tension, thereby achieving the purpose of evenly adding reactants.
[0058] It should be noted that the above-mentioned reactants may be antibodies, antigens, enzyme substrates, color developing solutions, luminescent solutions, PCR reactants, enzymes, etc.; the reactants may be one or more; when there are more than one reactant, they may be mixed and added at once, or may be added sequentially;
[0059] The position of the pores of the mask plate 1, the height of the frame, the width, depth and quantity of the mask strips 3, and the gaps between the mask strips 3 can all be adjusted according to customer requirements.
[0060] The above is only the preferred specific embodiment of the present utility model; however, the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, making equivalent substitutions or changes according to the technical solution and its improved concept of the present utility model, shall be covered by the protection scope of the present utility model.
Claims
1. An electrophoretic mask device, characterized in that: include: A mask plate having a plurality of adding holes formed thereon; A mask frame, which is arranged on the side with the smaller opening of the adding hole and plays a supporting role; The mask strip is a guide groove, and a through hole for communicating with the small end of the adding hole opening is provided on the back of the opening of the mask strip; The mask frame and the mask strip form a height difference, and the height of the mask strip is lower than the height of the mask frame; The liquid enters the guide groove from the adding hole and spreads evenly in the guide groove.
2. The electrophoretic mask device according to claim 1, characterized in that: Also includes: The positioning device is arranged at the bottom of the mask plate and is used to accurately place the mask plate above the film.
3. The electrophoretic mask device according to claim 1, characterized in that: Also includes: A handle is arranged on the upper part of the mask plate.
4. The electrophoretic mask device according to claim 1, characterized in that: The adding hole is at least one of a conical hole, a circular hole, a square hole and an irregular hole.
5. The electrophoretic mask device according to claim 4, characterized in that: The adding hole is a tapered hole.
6. The electrophoretic mask device according to claim 1, characterized in that: The height difference between the mask frame and the mask strip is 0.05 mm to 5 mm.
7. The electrophoretic mask device according to claim 6, characterized in that: The height difference between the mask frame and the mask strip is 0.1-2 mm.
8. An electrophoresis mask device according to any one of claims 1 to 7, characterized in that: The material of the mask device is transparent material.