Bioreactor

Abstract

The utility model relates to the field of biotechnology equipment, in particular to a bioreactor which comprises a reaction tank, a control cabinet, a pipeline system, a stirring system and a detection feedback system. The reaction tank comprises a tank body and a tank cover; the control cabinet comprises a casing, and a touch screen and a control host which are arranged in the casing, and the touch screen is connected with the control host; the new detection feedback system is mounted in the reaction tank and is connected with the control host; the detection feedback system comprises a dissolved oxygen electrode, a PH electrode, a turbidity electrode, a temperature electrode and a liquid level electrode, and all the electrodes detect information in the reaction tank and feed back and display the information to a touch screen connected with the control host. The reaction tank is responsible for culturing biological cells, information collected by electrodes in the reaction tank is fed back to the control host part, the host part can adjust various numerical values in the reaction tank by controlling different culture solutions and culture gases according to designed parameter values, and the automatic culture of various microbial cells is efficiently and accurately adapted.

Description

Technical Field

[0001] This application relates to the field of biotechnology equipment, and specifically to a bioreactor and a centrifugation system. Background Art

[0002] Cell culture is an essential important link in the field of biotechnology. A large number of cells or their metabolites are obtained through cell culture. The existing biological cell culture system has weak information detection ability in cell culture. Although it can be used for biological cell culture, there are problems of low culture efficiency and low survival rate. Utility Model Content

[0003] To achieve the above object, this application provides a bioreactor to overcome the problems of weak ability to detect cell culture information, low survival rate, and low culture efficiency in the background art.

[0004] This application provides the following technical solutions:

[0005] A bioreactor, comprising: a reaction tank, a control cabinet, a pipeline system, a stirring system, and a detection and feedback system;

[0006] The reaction tank includes a tank body and a tank cover. The tank cover is provided with a liquid inlet, a liquid outlet, a gas inlet, and a detection interface;

[0007] The control cabinet includes a cabinet body, a touch screen and a control host arranged in the cabinet body. The touch screen is connected to the control host;

[0008] The pipeline system is installed in the cabinet body and communicated with the reaction tank; the pipeline system is used to transfer the culture solution and the culture gas to the reaction tank to form an environment required for biological cell culture in the reaction tank;

[0009] The stirring system includes: a stirring shaft, a stirring paddle, and a stirring motor arranged on the tank cover; one end of the stirring shaft is connected to the output end of the stirring motor, the other end of the stirring shaft penetrates through the tank cover and is arranged in the tank body, and the stirring paddle is arranged on the stirring shaft;

[0010] The detection and feedback new system is installed on the reaction tank and connected to the control host; the detection and feedback system includes a dissolved oxygen electrode, a PH electrode, a turbidity electrode, a temperature electrode, and a liquid level electrode. Each electrode detects the information in the reaction tank and feeds back and displays it on the touch screen connected to the control host.

[0011] Further, the pipeline system includes a peristaltic pump, a bubble sensor, a pipe pressure valve, a gas pipeline, and a liquid pipeline;

[0012] The bubble sensor is used to detect the state of the pipeline in the pipeline system. The peristaltic pump is the power source of the entire pipeline system. The pipe pressure valve is used to control the on-off between each pipeline; the gas source chamber required for culture is communicated with the reaction tank through the gas pipeline; the reagent bag required for culture is communicated with the reaction tank through the liquid pipeline.

[0013] Furthermore, the stirring system further includes an antifoaming paddle. A stirring paddle is provided at the lower part of the stirring shaft, and an antifoaming paddle is provided at the upper part of the stirring shaft.

[0014] Furthermore, a refrigerating box is also provided in the casing of the control cabinet to collect and store culture reagents; the gas pipeline is connected to the air inlet of the tank cover through a distributor.

[0015] Furthermore, the tank body is made of a transparent material.

[0016] Furthermore, the bioreactor of the present application further includes a temperature control system for controlling the temperature of the tank body.

[0017] Furthermore, the temperature control system includes a heating blanket wrapped around the outer wall of the tank body and a cooling pipe provided inside the tank body; the heating blanket is used to heat up the tank body, and the cooling pipe is used to cool down the tank body.

[0018] Furthermore, a sampling port and an inoculation port are provided on the tank cover.

[0019] Furthermore, a condenser tube is also installed on the tank cover.

[0020] Furthermore, the bioreactor of the present application further includes a weighing chassis for supporting the tank body.

[0021] Advantageous effects:

[0022] A bioreactor of the present application includes a reaction tank, a control cabinet, a pipeline system, a stirring system, and a detection and feedback system; the reaction tank includes a tank body and a tank cover, and the tank cover is provided with a liquid inlet, a liquid outlet, a gas inlet, and a detection interface; the control cabinet includes a cabinet body, a touch screen and a control host arranged in the cabinet body, and the touch screen is connected to the control host; the pipeline system is installed in the cabinet body and communicated with the reaction tank; the pipeline system is used to transfer the culture medium and the culture gas to the reaction tank to form an environment required for biological cell culture in the reaction tank; the stirring system includes: a stirring shaft, a stirring paddle, and a stirring motor arranged on the tank cover; one end of the stirring shaft is connected to the output end of the stirring motor, the other end of the stirring shaft penetrates through the tank cover and is arranged in the tank body, and the stirring paddle is arranged on the stirring shaft; the detection and feedback new system is installed on the reaction tank and connected to the control host; the detection and feedback system includes a dissolved oxygen electrode, a PH electrode, a turbidity electrode, a temperature electrode, and a liquid level electrode, and each electrode detects the information in the reaction tank and feeds it back and displays it on the touch screen connected to the control host. The reaction tank is responsible for the culture of biological cells, and at the same time, the information collected by each electrode in the reaction tank is fed back to the control host part to realize the detection of the real-time state of cell culture; the host part is equipped with a touch screen for setting culture parameters and displaying real-time data of various detections; the host part can adjust various values in the reaction tank by controlling the addition of different culture media and culture gases according to the designed parameter values, efficiently and accurately adapting to the automated culture of various microbial cells, and adding the required culture gases and culture media through a closed pipeline system to effectively avoid the problem of contamination of the cultured cells in the reaction tank by the external environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0024] Figure 1 It is a schematic diagram of the structure of a bioreactor according to this embodiment;

[0025] Figure 2 It is a schematic top view structure diagram of the reaction tank in the bioreactor according to this embodiment;

[0026] Figure 3 is Figure 2 the internal structure schematic diagram of the A-A section of the reaction tank in;

[0027] In the figure: 1. Tank body, 2. Tank cover, 3. Stirring motor, 4. Stirring shaft, 5. Stirring paddle, 6. Defoaming paddle, 7. Coupling, 8. Touch screen, 9. Cooling pipe, 10. Liquid inlet, 11. Liquid outlet, 12. Air inlet, 13. Inoculation port, 14. Sampling port, 15. Dissolved oxygen electrode, 16. PH electrode, 17. Turbidity electrode, 18. Liquid level electrode, 19. Filtered liquid outlet, 20. Condenser tube, 21. Sealing strip, 22. Peristaltic pump, 23. Bubble sensor, 24. Pipe pressure valve, 25. Acid interface, 26. Alkaline interface, 27. Weighing chassis. Detailed implementation manners

[0028] In order to enable those skilled in the art to better understand the technical solutions in this application, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the scope of protection of this application.

[0029] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly disposed on the other element; when an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0030] It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to this application.

[0031] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of this application, the meanings of "plural" and "several" are two or more, unless otherwise specifically defined.

[0032] It should be noted that the structures, proportions, sizes, etc. shown in the attached drawings of this specification are only used to cooperate with the content disclosed in the specification for those familiar with this technology to understand and read, and are not used to limit the implementation conditions of this application. Therefore, they do not have any technical substance. Any modification of the structure, change in the proportional relationship, or adjustment of the size, without affecting the effects that this application can produce and the purposes that can be achieved, should still fall within the scope covered by the technical content disclosed in this application.

[0033] The embodiments of this application are written in a progressive manner.

[0034] Please refer to Figures 1 to 3 , a bioreactor, comprising: a reaction tank, a control cabinet, a pipeline system, a stirring system, and a detection and feedback system;

[0035] The reaction tank includes a tank body 1 and a tank cover 2. The tank cover is provided with a liquid inlet 10, a liquid outlet 11, a gas inlet 12, and a detection interface;

[0036] The control cabinet includes a cabinet body, and a touch screen 8 and a control host arranged in the cabinet body. The touch screen is connected to the control host;

[0037] The pipeline system is installed in the cabinet body and is communicated with the reaction tank; the pipeline system is used to transfer the culture solution and culture gas to the reaction tank to form an environment required for biological cell culture in the reaction tank;

[0038] The stirring system includes: a stirring shaft 4, a stirring paddle 5, and a stirring motor 3 arranged on the tank cover; one end of the stirring shaft is connected to the output end of the stirring motor, the other end of the stirring shaft penetrates through the tank cover and is arranged in the tank body, and the stirring paddle is arranged on the stirring shaft;

[0039] The detection and feedback new system is installed on the reaction tank and is connected to the control host; the detection and feedback system includes a dissolved oxygen electrode 15, a PH electrode 16, a turbidity electrode 17, a temperature electrode, and a liquid level electrode 18. Each electrode detects the information in the reaction tank and feeds it back and displays it on the touch screen connected to the control host.

[0040] In the above solution, the reaction tank is responsible for the culture of biological cells. At the same time, the information collected by each electrode in the reaction tank is fed back to the control host part to realize the detection of the real-time state of cell culture; the host part is equipped with a touch screen for setting culture parameters and displaying various real-time detection data; the host part can adjust the various values in the reaction tank by controlling the addition of different culture solutions and culture gases according to the designed parameter values, efficiently and accurately adapting to the automated culture of various microbial cells, and adding the required culture gas and culture solution through a closed pipeline system to effectively avoid the problem of the cultured cells in the reaction tank being contaminated by the external environment.

[0041] As a preferred embodiment, the pipeline system includes a peristaltic pump 22, a bubble sensor 23, a tube pressure valve 24, a gas pipeline and a liquid pipeline; the bubble sensor is used to detect the state of the pipelines in the pipeline system, the peristaltic pump is the power source of the entire pipeline system, and the tube pressure valve is used to control the on-off between each pipeline; the gas source chamber required for cultivation is connected to the reaction tank through the gas pipeline; the reagent bag required for cultivation is connected to the reaction tank through the liquid pipeline.

[0042] More preferably, a sealing strip 21 is also provided between the tank lid and the tank body for sealed connection, which ensures the cleanliness in the reaction tank. After the culture is placed in the glass tank, the nutrient solution and gas environment required for cultivation are transported and added through the pipeline system, further ensuring the problem of contamination of the cultured cells in the reaction tank by the external environment.

[0043] As a preferred embodiment, the stirring system further includes an antifoaming paddle 6. A stirring paddle is provided at the lower part of the stirring shaft, and an antifoaming paddle is provided at the upper part of the stirring shaft. More preferably, the output shaft of the stirring motor is connected to the stirring shaft through a coupling 7. The stirring paddle extends into the reaction tank, and the motor drives the stirring shaft to ensure that the nutrient solution is evenly distributed in the tank body. At the same time, an antifoaming paddle is arranged at the upper part of the stirring shaft, which can be used to eliminate the bubbles generated during the cultivation process; the stirring paddle is preferably an ordinary three-blade paddle and is arranged at the lower end of the stirring shaft.

[0044] As a preferred embodiment, the gas pipeline is connected to the air inlet of the tank lid through a distributor to add gases such as carbon dioxide, oxygen, and air required for cultivation to the reaction tank.

[0045] As an alternative embodiment, the tank body is a transparent material tank body, preferably a glass tank; the transparent glass tank is conducive to observing cell culture and the internal working conditions of the equipment.

[0046] As an alternative embodiment, the bioreactor of the present application further includes a temperature control system for controlling the temperature of the tank body. Further, the temperature control system includes a heating blanket wrapped around the outer wall of the tank body and a cooling pipe 9 arranged in the tank body; the heating blanket is used to heat up the tank body, and the cooling pipe is used to cool down the tank body. The temperature control system mainly controls the temperature of the reaction tank to keep the temperature in the tank within the appropriate temperature range for cell growth, ensuring that the temperature in the tank body always remains within the appropriate temperature for cell generation during the cultivation process. The cooling pipe is externally connected to a cooling system for cooling, the heating blanket is a heating electric blanket, and it is heated by being powered on; the temperature electrode in the reaction tank detects and feeds back the temperature information.

[0047] More preferably, a condenser tube 20 is also installed on the tank lid; the condenser tube is used to recover the liquid evaporated due to heating during the cultivation process, and a flowing low-temperature liquid is kept in the condenser tube to condense the evaporated gas.

[0048] As a preferred embodiment, a sampling port 14 and an inoculation port 13 are provided on the tank cover; the sampling port can be used to sample at any time through an external sampling device to observe the cell growth status in the tank; in addition, for liquids with poor fluidity, they can be added separately through the inoculation port to prevent blockage of the pipeline system.

[0049] As a preferred embodiment, an acidic interface 25, a basic interface 26 and a filtered liquid outlet 19 are further provided on the tank cover, and the structure of the filtered liquid outlet is that a filter is provided on the basis of the liquid outlet.

[0050] As a preferred embodiment, the bioreactor of the present application further includes a weighing chassis 27 for supporting the tank body. The weighing chassis is electrically connected to the control host, and can realize weight monitoring during cell culture and centrifugation.

[0051] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present application. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A bioreactor, characterized in that: include: Reaction tank, control cabinet, piping system, stirring system and detection feedback system; The reaction tank comprises a tank body and a tank cover, and the tank cover is provided with a liquid inlet, a liquid outlet, an air inlet and a detection interface; The control cabinet includes a housing, a touch screen and a control host arranged in the housing, and the touch screen is connected to the control host; The pipeline system is installed on the casing and is connected with the reaction tank; The pipeline system is used to transfer the culture fluid and culture gas to the reaction tank to form the environment required for biological cell culture in the reaction tank; The stirring system includes: a stirring shaft, a stirring paddle and a stirring motor arranged on the tank cover; one end of the stirring shaft is connected to the output end of the stirring motor, the other end of the stirring shaft penetrates the tank cover and is arranged in the tank body, and a stirring paddle is arranged on the stirring shaft; The new detection and feedback system is installed in the reaction tank and connected to the control host; the detection and feedback system includes a dissolved oxygen electrode, a pH electrode, a turbidity electrode, a temperature electrode and a liquid level electrode. Each electrode detects the information in the reaction tank and feeds back the information to a touch screen connected to the control host.

2. The bioreactor according to claim 1, characterized in that The pipeline system includes a peristaltic pump, a bubble sensor, a pipe pressure valve, a gas pipeline and a liquid pipeline; The bubble sensor is used to detect the status of the pipeline in the pipeline system, the peristaltic pump is the power source of the entire pipeline system, and the pipeline pressure valve is used to control the on-off of each pipeline; the gas source chamber required for culture is connected to the reaction tank through the gas pipeline; the reagent bag required for culture is connected to the reaction tank through the liquid pipeline.

3. The bioreactor according to claim 1, characterized in that The stirring system also includes a defoaming paddle, a stirring paddle is arranged at the lower part of the stirring shaft, and a defoaming paddle is arranged at the upper part of the stirring shaft.

4. The bioreactor according to claim 1, characterized in that A refrigerator is also provided in the casing of the control cabinet to collect and store culture reagents.

5. The bioreactor according to claim 1, characterized in that The tank body is made of transparent material.

6. The bioreactor according to any one of claims 1 to 5, characterized in that: A temperature control system for controlling the temperature of the tank is also included.

7. The bioreactor according to claim 6, characterized in that The temperature control system includes a heating blanket wrapped around the outer wall of the tank body and a cooling pipe arranged inside the tank body; the heating blanket is used to heat the tank body, and the cooling pipe is used to cool the tank body.

8. The bioreactor according to any one of claims 1 to 5 and 7, characterized in that: The tank cover is provided with a sampling port and an inoculation port.

9. The bioreactor according to any one of claims 1 to 5 and 7, characterized in that: A condenser is also installed on the tank cover.

10. The bioreactor according to any one of claims 1 to 5 and 7, characterized in that: Also included is a weighing chassis for supporting the tank.

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