Rotor structure for protein separation equipment

Abstract

The utility model relates to the technical field of protein separation equipment matching structures, in particular to a protein separation equipment rotor structure which comprises a shell, a middle sealing sleeve, a top sealing cover, a liquid outlet hole, a liquid adding pipe, a bottom sealing sleeve, an outer pipe body, a liquid outlet pipe, a movable sealing sleeve, a connecting sleeve and a fixing rod. The middle sealing sleeve is connected to the upper end of the shell in a matched mode, the top sealing cover is connected to the upper end of the middle sealing sleeve in a matched mode, the lower end of the top sealing cover is connected with the outer surface of the upper end of the shell in a matched mode, and the liquid outlet holes are formed in the surface, close to the lower end, of the middle sealing sleeve in an annular array penetrating mode. The interior of the middle sealing sleeve is a cavity, the liquid adding pipe is of a double-layer pipe body structure, the lower end of the liquid adding pipe extends into the lower end of the middle sealing sleeve, and the device has the advantages that the operation convenience degree is high, the top is not prone to leakage, the real-time separation requirement is well met, and the separation effect is good.

Description

Technical Field

[0001] This application relates to the technical field of supporting structures for protein separation equipment, and particularly relates to a rotor structure for protein separation equipment. Background Art

[0002] In the manufacturing process of biopharmaceuticals, it is necessary to separate blood raw materials, so that the cells and serum inside are separated, and the large-volume components and serum in the blood are collected separately. Among the existing separation methods, the centrifugation form is preferred, and it is widely used because of its advantages of easy operation and good safety. In the existing separation equipment, for some centrifugal equipment with real-time feeding and discharging, after adding blood into the conical rotor, under the action of centrifugal force, the serum will move upward along the inner wall of the rotor, so as to achieve separation and collection. However, the existing rotor centrifugal separation device still has problems such as easy leakage at the top and inconvenient operation of adding and collecting liquids, so it still needs to be improved. Summary of the Invention

[0003] Aiming at the deficiencies of the existing technology, the purpose of this application is to provide a rotor structure for protein separation equipment with high operation convenience, not easy to leak at the top, and good separation effect.

[0004] The above application purpose of this application is achieved through the following technical solutions:

[0005] A rotor structure for protein separation equipment includes: a housing, a middle sealing sleeve, a top sealing cover, a liquid outlet hole, a liquid adding pipe, a bottom sealing sleeve, an outer pipe body, a liquid outlet pipe, a movable sealing sleeve, a connecting sleeve and a fixing rod. The diameter of the lower end of the housing is larger than that of the upper end. The middle sealing sleeve is connected to the upper end of the housing in a matching manner. The top sealing cover is connected to the upper end of the middle sealing sleeve in a matching manner. The lower end of the top sealing cover is connected to the outer surface of the upper end of the housing in a matching manner. The liquid outlet holes are arranged in an annular array and penetrate through the surface of the middle sealing sleeve near the lower end. The inside of the middle sealing sleeve is a cavity. The liquid adding pipe is a double-layer pipe structure. The lower end of the liquid adding pipe extends into the lower end of the middle sealing sleeve. The bottom sealing sleeve is rotatably connected to the middle of the middle sealing sleeve. The lower end of the liquid adding pipe is connected to the middle of the bottom sealing sleeve in a matching manner. The outer pipe body is arranged outside the liquid adding pipe corresponding to the cavity inside the middle sealing sleeve. The liquid outlet pipe is connected to the upper end of the outer pipe body in a penetrating manner. The movable sealing sleeve is connected to the upper end of the middle position of the top sealing cover in a matching manner. The movable sealing sleeve is rotatably connected to the top sealing cover. The outer surface of the outer pipe body is connected to the middle of the movable sealing sleeve in a matching manner. The connecting sleeve is fixedly connected to the upper end of the liquid adding pipe. One end of the fixing rod is fixedly connected to the connecting sleeve.

[0006] Optionally, it further includes a conical protrusion, and the conical protrusion is fixedly connected to the bottom surface inside the housing.

[0007] Optionally, it further includes a storage tank, and the storage tank is arranged on the inner wall near the edge at the lower end of the housing.

[0008] Optionally, it further includes a connection notch, and the connection notch is equidistantly arranged in an annular array on the outer surface at the lower end of the housing.

[0009] Optionally, it further includes a liquid inlet conduit, and the liquid inlet conduit is connected through and at the upper end of the liquid adding tube.

[0010] Optionally, it further includes a mounting groove, and the mounting groove is arranged at the middle position at the lower end of the housing.

[0011] Optionally, it further includes a liquid guiding slope, and the liquid guiding slope is arranged at the edge position near the lower end of the middle sealing sleeve.

[0012] Optionally, it further includes a clamping groove, and the clamping groove is arranged on the outer surface in the middle of the middle sealing sleeve, and the clamping groove is in fit connection with the edge at the upper end of the housing.

[0013] The rotor structure for the protein separation device can achieve continuous separation of the added blood through a combined structure, enabling large molecular substances such as cells to be separated from the serum, deposited at the bottom, and the serum to be discharged from the upper end, realizing continuous addition and continuous separation processes, and ensuring a faster separation effect.

[0014] The rotor structure for the protein separation device has good sealing performance after installation, and it is not easy to leak at the upper connection position, making the operation station easier to always remain clean, avoiding contamination caused by leaked serum, and meeting the hygiene requirements for the production of blood products.

[0015] The rotor structure for the protein separation device is convenient for collecting the separated serum during use. Only by placing the collection container at the end of the liquid outlet tube can the collection process be realized, which is convenient for operation, simpler to use, and has good practicability. Description of the Drawings

[0016] Figure 1 is a schematic diagram of the overall partial sectional structure provided by an embodiment of the present application;

[0017] Figure 2 is a schematic diagram of the bottom structure provided by an embodiment of the present application;

[0018] Figure 3 is a schematic diagram of the sectional structure of the liquid adding tube provided by an embodiment of the present application;

[0019] Figure 4 is a schematic diagram of the sectional structure of the middle sealing sleeve provided by an embodiment of the present application;

[0020] Figure 5It is a schematic cross-sectional view of the top sealing cover provided by the embodiment of the present application.

[0021] Reference numerals: 1, housing; 2, middle sealing sleeve; 3, top sealing cover; 4, liquid outlet hole; 5, liquid adding pipe; 6, bottom sealing sleeve; 7, outer pipe body; 8, liquid outlet pipe; 9, movable sealing sleeve; 10, connecting sleeve; 11, fixed rod; 12, conical protrusion; 13, storage tank; 14, connecting notch; 15, liquid inlet conduit; 16, installation groove; 17, liquid guiding slope; 18, clamping groove. Detailed implementation manners

[0022] The following further elaborates on the present application with reference to the accompanying drawings.

[0023] To more clearly understand the technical solutions presented in the embodiments of the present application, first, the working principle of the existing rotor structure for protein separation equipment will be introduced.

[0024] In existing protein separation equipment, most separation devices are in the form of rotors. Some use the form of directly placing test tubes in them for overall rotation separation, and some use the method of placing the container filled with blood into the rotor as a whole. Under high-speed rotation, blood cells can be deposited at the bottom. However, the number of separations in one operation of this type of device is limited. For some cases where a large volume of blood needs to be separated, the separation time required for this type of separation equipment is relatively long. Therefore, a continuous separation centrifuge device is more suitable. Since the volume of blood cells contained in blood is small, it is not necessary to remove blood cells when continuously separating a large volume of blood. However, in the existing rotor structure of this kind, the top is not tightly sealed, prone to leakage, and sometimes even jet-like leakage occurs during rotation, contaminating the working station with serum. Therefore, it is necessary to improve the existing rotor structure to solve these problems.

[0025] Please refer to Figures 1 to 5, a rotor structure for a protein separation device disclosed in an embodiment of the present application, comprising: a housing 1, a middle seal sleeve 2, a top seal cover 3, a liquid outlet hole 4, a liquid adding pipe 5, a bottom seal sleeve 6, an outer pipe body 7, a liquid outlet pipe 8, a movable seal sleeve 9, a connecting sleeve 10 and a fixing rod 11. The diameter of the lower end of the housing 1 is larger than that of the upper end. The middle seal sleeve 2 is fitted and connected to the upper end of the housing 1. The top seal cover 3 is fitted and connected to the upper end of the middle seal sleeve 2. The lower end of the top seal cover 3 is fitted and connected to the outer surface of the upper end of the housing 1. The liquid outlet holes 4 are arranged in an annular array and penetrate through the surface of the middle seal sleeve 2 near the lower end. The inside of the middle seal sleeve 2 is a cavity. The liquid adding pipe 5 is a double-layer pipe structure. The lower end of the liquid adding pipe 5 extends into the lower end of the middle seal sleeve 2. The bottom seal sleeve 6 is rotatably connected to the middle of the middle seal sleeve 2. The lower end of the liquid adding pipe 5 is fitted and connected to the middle of the bottom seal sleeve 6. The outer pipe body 7 is arranged outside the liquid adding pipe 5 corresponding to the cavity inside the middle seal sleeve 2. The liquid outlet pipe 8 is connected to the upper end of the outer pipe body 7 in a penetrating manner. The movable seal sleeve 9 is fitted and connected to the upper end of the middle position of the top seal cover 3. The movable seal sleeve 9 is rotatably connected to the top seal cover 3. The outer surface of the outer pipe body 7 is fitted and connected to the middle of the movable seal sleeve 9. The connecting sleeve 10 is fixedly connected to the upper end of the liquid adding pipe 5. One end of the fixing rod 11 is fixedly connected to the connecting sleeve 10.

[0026] Specifically, the upper port of the provided housing 1 has a smaller diameter and the lower end has a larger diameter, which can generate a greater centrifugal force during rotation. The provided middle seal sleeve 2 can be connected to the inlet position at the upper end of the housing 1, enabling the contact surface between the two to achieve a sealed connection. The provided top seal cover 3 can be connected to the upper end of the middle seal sleeve 2, and further, the lower end can also be connected to the outer wall of the housing 1, forming a relatively tight closed effect among them. During actual use, components such as rubber rings are also provided between the connecting surfaces to enhance the sealing effect after connection. The provided liquid outlet hole 4 can collect the serum that floats upward through centrifugal force, enabling the serum to converge in the middle seal sleeve 2 through the liquid outlet hole 4, and then be discharged to the liquid outlet pipe 8 through the outer pipe body 7. The provided liquid adding pipe 5 can conduct the external blood and introduce it into the housing 1 at a certain speed, thereby achieving continuous feeding. The provided bottom seal sleeve 6 can connect the lower end of the liquid adding pipe 5 to the middle seal sleeve 2. Then, when the middle seal sleeve 2 rotates, the liquid adding pipe 5 is kept from rotating and its connection tightness is ensured. The provided movable seal sleeve 9 can be connected to the surface of the outer pipe body 7, and at the same time, ensure that there is no leakage at the connection position during the rotation of the top seal cover 3, enabling the serum to be continuously discharged outward through the outer pipe body 7. The provided connecting sleeve 10 can connect the upper end of the liquid adding pipe 5, and then be fixed on the support structure through the fixing rod 11 to avoid the liquid adding pipe 5 from rotating. This structure can achieve a continuous and stable separation effect, effectively handle large-volume separation scenarios, and ensure a good sealing effect. Compared with the existing rotor structure, it can better maintain the cleanliness of the workbench surface and ensure the safety of operation.

[0027] Please refer to Figure 1 , as another specific implementation provided by the application, it further includes a conical protrusion 12, and the conical protrusion 12 is fixedly connected to the bottom surface inside the housing 1.

[0028] Specifically, the setting of the conical protrusion 12 can disperse the blood introduced by the liquid adding pipe 5 to the surrounding, facilitating the achievement of a uniform separation effect.

[0029] Please refer to Figure 1 , as another specific implementation provided by the application, it further includes a storage tank 13, and the storage tank 13 is arranged on the inner wall near the edge at the lower end of the housing 1.

[0030] Specifically, the setting of the storage tank 13 can store and collect substances with a large weight such as blood cells, causing them to deposit at the bottom position of the housing 1.

[0031] Please refer to Figure 2 , as another specific implementation provided by the application, it further includes a connection notch 14, and the connection notch 14 is annularly arrayed and equally spaced on the outer surface at the lower end of the housing 1.

[0032] Specifically, the connection notch 14 is provided to connect with an external driving device, providing a clamping position to ensure stable connection.

[0033] Please refer to Figure 3 , as another specific implementation provided by the application, it further includes a liquid inlet conduit 15, and the liquid inlet conduit 15 is connected through the upper end of the liquid adding tube 5.

[0034] Specifically, the liquid inlet conduit 15 is provided to introduce an external blood sample, so that it continuously enters the liquid adding tube 5. During actual use, it also needs to have a certain pressure value to avoid being extruded in the reverse direction.

[0035] Please refer to Figure 4 , as another specific implementation provided by the application, it further includes a mounting groove 16, and the mounting groove 16 is arranged at the middle position of the lower end of the housing 1.

[0036] Specifically, the mounting groove 16 is provided to connect with an existing rotor driving structure, improving adaptability.

[0037] Please refer to Figure 4 , as another specific implementation provided by the application, it further includes a liquid guiding ramp 17, and the liquid guiding ramp 17 is arranged at the edge position near the lower end of the middle sealing sleeve 2.

[0038] Specifically, the liquid guiding ramp 17 is provided to enable the upward - moving serum to directly enter the liquid outlet hole 4 along the inner wall of the housing 1, improving the smoothness of its discharge, reducing the number of reciprocating flows inside, and improving the collection efficiency.

[0039] Please refer to Figure 4 , as another specific implementation provided by the application, it further includes a clamping groove 18, and the clamping groove 18 is arranged on the outer surface of the middle part of the middle sealing sleeve 2, and the clamping groove 18 is connected in cooperation with the edge of the upper end of the housing 1.

[0040] Specifically, the clamping groove 18 is provided to cooperate with the edge position of the upper end of the housing 1, and components such as rubber pads and rubber rings are arranged between the connection surfaces to ensure the sealing effect after connection.

[0041] The embodiments of this specific implementation are all preferred embodiments of this application. It does not limit the protection scope of this application accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A rotor structure for protein separation equipment, characterized in that: include: A shell (1), a middle sealing sleeve (2), a top sealing cover (3), a liquid outlet hole (4), a liquid adding pipe (5), a bottom sealing sleeve (6), an outer tube body (7), a liquid outlet pipe (8), a movable sealing sleeve (9), a connecting sleeve (10) and a fixing rod (11); the diameter of the lower end of the shell (1) is greater than the diameter of the upper end; the middle sealing sleeve (2) is connected to the upper end of the shell (1); the top sealing cover (3) is connected to the upper end of the middle sealing sleeve (2); the lower end of the top sealing cover (3) is connected to the outer surface of the upper end of the shell (1); the liquid outlet hole (4) is formed in an annular array and is provided on the surface of the middle sealing sleeve (2) near the lower end; the interior of the middle sealing sleeve (2) is a cavity; the liquid adding pipe (5) is a double-layer pipe structure; the liquid adding pipe (5) The lower end extends into the lower end of the middle sealing sleeve (2); the bottom sealing sleeve (6) is rotatably connected to the middle of the middle sealing sleeve (2); the lower end of the liquid adding pipe (5) is cooperatively connected to the middle of the bottom sealing sleeve (6); the outer tube body (7) is arranged outside the liquid adding pipe (5) corresponding to the internal cavity of the middle sealing sleeve (2); the liquid outlet pipe (8) is connected through the upper end of the outer tube body (7); the movable sealing sleeve (9) is cooperatively connected to the upper end of the middle position of the top sealing cover (3); the movable sealing sleeve (9) is rotatably connected to the top sealing cover (3); the outer surface of the outer tube body (7) is cooperatively connected to the middle of the movable sealing sleeve (9); the connecting sleeve (10) is fixedly connected to the upper end of the liquid adding pipe (5); and one end of the fixing rod (11) is fixedly connected to the connecting sleeve (10).

2. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises a conical protrusion (12), wherein the conical protrusion (12) is fixedly connected to the bottom surface inside the shell (1).

3. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises a material storage trough (13), wherein the material storage trough (13) is arranged on the inner wall of the lower end of the shell body (1) close to the edge.

4. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises connecting notches (14), wherein the connecting notches (14) are arranged in an annular array and are equidistantly arranged on the surface of the outer side of the lower end of the shell (1).

5. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises a liquid inlet conduit (15), wherein the liquid inlet conduit (15) is connected to the upper end of the liquid adding pipe (5).

6. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises a mounting groove (16), wherein the mounting groove (16) is arranged at a middle position of the lower end of the housing (1).

7. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises a liquid guiding slope (17), wherein the liquid guiding slope (17) is arranged at an edge position of the middle sealing sleeve (2) close to the lower end.

8. A rotor structure for protein separation equipment according to claim 1, characterized in that: It also comprises a card slot (18), wherein the card slot (18) is arranged on the outer surface of the middle part of the middle sealing sleeve (2), and the card slot (18) is matched and connected with the edge of the upper end of the shell (1).

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