Incubator for arbuscular mycorrhizal fungi

By designing an incubator containing the main body, a sealing rack, a culture plate and a culture box, the problem of inconvenience of fungal root entanglement and sampling is solved, and the stable placement of multiple sets of culture boxes and separate sampling experiments are achieved, which is convenient for labeling and experimental operations.

CN223047483UActive Publication Date: 2025-07-01ZHAODONG QING EAST FERTILIZER IND
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Patent Information

Application Number
CN202421555689.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-07-03
Publication Date
2025-07-01
Estimated Expiration
2034-07-03

AI Technical Summary

Technical Problem

When the existing incubator cultivates arbuscular mycorrhizal fungi, the fungal root systems are prone to wrap around and grow, which affects the sampling operation and makes it difficult to take samples separately.

Method used

An incubator including the main body, a sealing rack, a culture plate and a cultivation box was designed. The placement space was formed by an equidistantly arranged disc rack and a baffle rack. Each group of incubator boxes was separated for easy labeling, and the incubator box was fixed through the slot and the water seepage hole to avoid root entanglement.

Benefits of technology

This incubator can accommodate multiple sets of incubation boxes at one time, maintain stability, avoid entanglement of fungal roots, facilitate separate sampling experiments, and improve the convenience and efficiency of the experiment.

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Abstract

The utility model discloses a culture box for arbuscular mycorrhizal fungi, which belongs to the technical field of arbuscular mycorrhizal fungi culture tests, and comprises a main body, a sealing plate frame is clamped at the front end of the main body, a culture tray is connected to the inner wall of the sealing plate frame, the culture tray comprises a tray frame, one end of the tray frame is connected with a glass frame, and the other end of the tray frame is connected with a light source. Baffle frames are distributed on the top face of the tray frame, clamping grooves are formed between the baffle frames, cultivation boxes are clamped to the periphery of the top face of each clamping groove, and water seepage holes are formed in the tray frame. According to the incubator, the placing space is formed by the tray racks which are distributed along the inner wall of the box sealing door at equal intervals, multiple sets of cultivation boxes can be contained at a time, the multiple sets of baffle racks distributed on the top faces of the tray racks are matched to separate the sets of cultivation boxes, marking and taking are convenient, each set of cultivation boxes are clamped and fixed to the top face through the clamping grooves, and stable placing is kept; single fungus plants are cultivated in the cultivation box, so that mutual winding growth of fungus root systems is avoided, and independent sampling experiments are facilitated.
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Description

Technical Field

[0001] The utility model relates to the technical field of arbuscular mycorrhizal fungi cultivation experiments, and particularly relates to an incubator for arbuscular mycorrhizal fungi. Background Technique

[0002] Arbuscular mycorrhizal fungi are a type of soil microorganism widely existing in nature. They form a symbiotic system with the roots of most plants and have a significant promoting effect on plant growth and development. It is a consortium formed by the mycelium of mycorrhizal fungi in the soil and the nutrient roots of higher plants. The symbiotic fungi obtain necessary carbohydrates and other nutrients from the plant, while the plant also gets the required nutrients and water from the fungi, thus achieving a high degree of unity of mutual benefit and mutual exchange. It has both the characteristics of general plant roots and the characteristics of obligate fungi. When conducting experiments on the cultivation of arbuscular mycorrhizal fungi mycelium, it is necessary to observe and analyze the growth state of plants and the influence of arbuscular mycorrhizal fungi mycelium on the soil. Generally, an incubator is used to cultivate arbuscular mycorrhizal fungi.

[0003] When the existing incubator conducts cultivation experiments on fungi, the cultivation process is generally carried out through the cultivation trays inside the incubator. When it is necessary to sample individual fungi, due to the growth of fungal roots, there is a phenomenon that the roots are intertwined and grow together, which affects the transplanting operation of sampling and is extremely inconvenient. Therefore, we propose an incubator for arbuscular mycorrhizal fungi. Content of the Utility Model

[0004] The content part of this application is used to briefly introduce the concepts, which will be described in detail in the subsequent specific implementation part. The content part of this application is not intended to identify the key features or essential features of the claimed technical solution, nor is it intended to limit the scope of the claimed technical solution.

[0005] The purpose of the utility model is to provide an incubator for arbuscular mycorrhizal fungi to solve the problems raised in the above background technique.

[0006] To achieve the above purpose, the utility model provides the following technical solution: An incubator for arbuscular mycorrhizal fungi, including a main body, a sealing plate frame is clamped at the front end of the main body, and a cultivation tray is connected to the inner wall of the sealing plate frame. The cultivation tray includes a tray frame, and a glass frame is connected to one end of the tray frame. Baffle frames are arranged on the top surface of the tray frame, and card slots are opened between the baffle frames. Cultivation boxes are clamped around the top surface of the card slots, and water seepage holes are opened inside the tray frame.

[0007] Furthermore, the tray frame and the glass frame are fixedly connected, and the tray frames are arranged equidistantly along the inner wall of the sealing plate frame.

[0008] Further, the cultivation boxes and the water seepage holes are arranged equidistantly along the top surface of the tray rack, and the cultivation boxes are engaged with the top surface of the tray rack through card slots.

[0009] Further, the main body includes a box body, ventilation openings are distributed on both sides of the box body, a water storage box is opened on one side of the bottom end of the box body, and a base is connected to the bottom end of the box body.

[0010] Further, the center of the base is in a hollow shape, and the water storage box is communicated with the inner bottom end of the box body.

[0011] Further, the sealing plate rack includes a sealing box door, handle racks are connected to both sides of the front end of the sealing box door, guide rail racks are distributed on both sides of the inner wall of the sealing box door, and a guide rail groove is opened on one side of the guide rail rack.

[0012] Further, the sealing box door is slidably matched with both sides of the inner wall of the box body through the guide rail rack and the guide rail groove, and the inside of the sealing box door is nested and fixed around the glass frame.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] In this incubator, a placement space is formed by the tray racks arranged equidistantly along the inner wall of the sealing box door, and multiple groups of cultivation boxes can be accommodated at one time. The multiple groups of baffle racks distributed on the top surface of the tray rack are used to separate the cultivation boxes in each group, which is convenient for labeling and taking. Each group of cultivation boxes is fixed on the top surface by card slots, keeping the placement stable. Each of these cultivation boxes cultivates a single strain of fungus, thus avoiding the intertwined growth of the fungal roots and being more conducive to individual sampling experiments;

[0015] This incubator uses the box body as the main carrier, and cooperates with the sealing plate rack and multiple cultivation trays connected and carried inside to carry out block cultivation on arbuscular mycorrhizal fungi, providing a stable planting and cultivation space. The water storage box at the bottom end can be arranged at the inner bottom end of the box body to provide a water storage function for it, avoiding the phenomenon of internal water seepage and water accumulation;

[0016] In this collection device, by installing a sealing box door at the front end of the box body, a sealed door can be formed. The stability of the pull of the sealing box door and the front end of the box body can be maintained by relying on the guide rail rack and the guide rail groove. At the same time, the structure of the sealing box door can keep the connection stability of the tray rack through the nested glass frame. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a three-dimensional structural schematic diagram of the main body of the present utility model;

[0018] Figure 2 is a front view structural schematic diagram of the shovel rack of the present utility model;

[0019] Figure 3 is a three-dimensional structural schematic diagram of the shovel platform in the shovel rack of the present utility model;

[0020] Figure 4 This is a schematic three-dimensional sectional view of the positioning pile of the present utility model.

[0021] In the figure: 1. Main body; 101. Box body; 102. Ventilation opening; 103. Water storage box; 104. Base; 2. Sealing plate frame; 201. Sealing box door; 202. Handle; 203. Guide rail frame; 204. Guide rail groove; 3. Culture dish; 301. Dish rack; 302. Glass frame; 303. Baffle frame; 304. Card slot; 305. Cultivation box; 306. Water seepage hole. Detailed implementation manners

[0022] Embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although some embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure can be implemented in various forms and should not be construed as limited to the embodiments set forth herein. On the contrary, these embodiments are provided to more thoroughly and completely understand the present disclosure. It should be understood that the drawings and embodiments of the present disclosure are only for exemplary purposes and are not used to limit the protection scope of the present disclosure.

[0023] In addition, it should be noted that for the sake of description, only parts related to the relevant invention are shown in the drawings. Without conflict, the embodiments in the present disclosure and the features in the embodiments can be combined with each other.

[0024] It should be noted that the concepts such as "first" and "second" mentioned in the present disclosure are only used to distinguish different devices, modules or units, and are not used to limit the order or interdependence relationship of the functions performed by these devices, modules or units.

[0025] It should be noted that the modifications of "one" and "multiple" mentioned in the present disclosure are illustrative rather than restrictive. Those skilled in the art should understand that unless otherwise clearly specified in the context, it should be understood as "one or more".

[0026] The present disclosure will be described in detail below with reference to the drawings and in combination with embodiments.

[0027] The present utility model provides a culture box for arbuscular mycorrhizal fungi as Figures 1-4 shown, which includes a main body 1. A sealing plate frame 2 is clamped at the front end of the main body 1, and a culture dish 3 is connected to the inner wall of the sealing plate frame 2. The main body 1 includes a box body 101, ventilation openings 102 are distributed on both sides of the box body 101, a water storage box 103 is opened on one side of the bottom end of the box body 101, and a base 104 is connected to the bottom end of the box body 101;

[0028] In order to maintain the stability of the overall culture box during the culture experiment, as Figure 1As shown, this culture box uses the box body 101 as the main carrier, cooperates with the sealing plate frame 2 and multiple groups of culture plates 3 connected and carried inside to carry out block cultivation of arbuscular mycorrhizal fungi, providing a stable planting and cultivation space, and the water storage box 103 at the bottom can be arranged at the bottom of the box body 101 to provide water storage function for it to avoid internal water seepage and accumulation.

[0029] like Figure 2 As shown, the sealing frame 2 includes a sealing door 201, and handle frames 202 are connected to both sides of the front end of the sealing door 201, and guide rail frames 203 are distributed on both sides of the inner wall of the sealing door 201, and a guide rail groove 204 is opened on one side of the guide rail frame 203;

[0030] In order to maintain the stability of the internal structure and facilitate the collection of fungal samples, Figure 2 As shown, in this collection device, a sealing door 201 is arranged at the front end of the box body 101 to form a sealing door. The sealing door 201 and the front end of the box body 101 can be stably pulled out by means of a guide rail frame 203 and a guide rail groove 204. At the same time, the sealing door 201 structure can maintain a stable connection with the disk rack 301 through a nested glass frame 302.

[0031] like Figure 4 As shown, the culture tray 3 includes a tray frame 301, and one end of the tray frame 301 is connected to a glass frame 302, a baffle frame 303 is arranged on the top surface of the tray frame 301, and a slot 304 is opened between the baffle frames 303, a culture box 305 is clamped around the top surface of the slot 304, and a water seepage hole 306 is opened inside the tray frame 301;

[0032] Finally, in order to facilitate sampling and cultivation, Figure 4 As shown, this device forms a placement space by means of racks 301 arranged equidistantly along the inner wall of the sealing door 201, which can accommodate multiple groups of culture boxes 305 at one time, and cooperates with multiple groups of baffle racks 303 distributed on the top surface of the racks 301 to separate the groups of culture boxes 305 for easy labeling and retrieval, and each group of culture boxes 305 is fixed to the top surface by a card slot 304 to keep the placement stable. Each of the culture boxes 305 is cultured with a single fungus, thereby avoiding the entanglement and growth of fungal roots, which is more conducive to individual sampling experiments.

[0033] In summary, when using this incubator, the fungi to be cultivated and the cultivation soil can be filled and planted in the cultivation box 305 first. Then, multiple groups of cultivation boxes 305 are distributed along the baffle frame 303 and are successively snap-fitted and fixed with the card slots 304 on the top surface of the tray rack 301. After the entire tray rack 301 has carried the fungi to be cultivated, the overall sealing door 201 is stably slid into the interior of the box body 101 along the two-side guide rail frames 203 and the guide rail grooves 204. Ventilation openings 102 are provided on both sides of this box body 101 to maintain the internal ventilation cultivation state. When individual sampling is required, just hold the handle frame 202 and slide the sealing door 201 out. Then, take out the individual cultivation box 305. When watering after pulling it out, the excess water will flow into the water storage box 103 below through the water seepage holes 306 for collection, and it can be taken out for treatment later.

[0034] The above description is only some preferred embodiments of the present disclosure and an explanation of the applied technical principles. Those skilled in the art should understand that the scope of the invention involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the above inventive concept. For example, the technical solutions formed by mutually replacing the above features with the (but not limited to) technical features with similar functions disclosed in the embodiments of the present disclosure.

Claims

1. A culture box for arbuscular mycorrhizal fungi, comprising a main body (1), characterized in that: The front end of the main body (1) is engaged with a plate sealing frame (2), and the inner wall of the plate sealing frame (2) is connected to a culture plate (3), wherein the culture plate (3) comprises a plate frame (301), and one end of the plate frame (301) is connected to a glass frame (302), a baffle frame (303) is arranged on the top surface of the plate frame (301), and slots (304) are provided between the baffle frames (303), a cultivation box (305) is engaged around the top surface of the slot (304), and a water seepage hole (306) is provided inside the plate frame (301).

2. The incubator for arbuscular mycorrhizal fungi according to claim 1, characterized in that: The tray rack (301) and the glass frame (302) are fixedly connected, and the tray rack (301) is arranged at equal distances along the inner wall of the sealing plate rack (2).

3. The incubator for arbuscular mycorrhizal fungi according to claim 1, characterized in that: The cultivation box (305) and the water seepage holes (306) are arranged at equal distances along the top surface of the tray rack (301), and the cultivation box (305) is engaged with the top surface of the tray rack (301) via the engagement groove (304).

4. The incubator for arbuscular mycorrhizal fungi according to claim 1, characterized in that: The main body (1) comprises a box body (101), and ventilation holes (102) are distributed on both sides of the box body (101). A water storage box (103) is provided on one side of the bottom end of the box body (101), and the bottom end of the box body (101) is connected to a base (104).

5. The incubator for arbuscular mycorrhizal fungi according to claim 4, characterized in that: The center of the base (104) is hollowed out, and the water storage box (103) is connected to the bottom end of the interior of the box body (101).

6. The incubator for arbuscular mycorrhizal fungi according to claim 1, characterized in that: The plate sealing frame (2) comprises a box sealing door (201), and handle frames (202) are connected to both sides of the front end of the box sealing door (201), and guide rail frames (203) are distributed on both sides of the inner wall of the box sealing door (201), and a guide rail groove (204) is opened on one side of the guide rail frame (203).

7. The culture box for arbuscular mycorrhizal fungi according to claim 6, characterized in that: The sealing door (201) is slidably matched with two sides of the inner wall of the box body (101) via the guide rail frame (203) and the guide rail groove (204), and the interior of the sealing door (201) is nested and fixed with the glass frame (302) around.