Microfluidic detection device

Abstract

The utility model relates to the technical field of detection, in particular to a microfluidic detection device. A microfluidic detection device comprises: a dilution cup, the bottom of which is provided with an insertion column groove, a liquid outlet hole communicated with the inner cavity of the dilution cup, and a pull ring film passing through the groove opening of the insertion column groove and covering the liquid outlet hole; the reagent disc is provided with a cup containing cavity which is open towards the top and is used for containing the dilution cup, the bottom of the reagent disc is provided with a connecting part, and the connecting part is provided with a first magnetic attraction piece, a guide opening communicated with the cup containing cavity and centering upper meshing teeth; and the fixed seat is provided with a guide film tearing column matched with the column inserting groove, a second magnetic attraction piece matched with the first magnetic attraction piece and centering lower grinding teeth matched with the centering upper grinding teeth. According to the scheme, the whole installation process is simple and efficient, accessories are few, the manufacturing cost is low, the cost is reduced, automatic centering is achieved, the steps of automatic centering installation of the reagent disc and film tearing can be achieved as long as the reagent disc is slightly pushed into the fixing base, and the installation efficiency and the convenience degree are effectively improved.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and particularly to a device for microfluidic detection. Background Art

[0002] Microfluidic detection technology can complete sample processing and detection on small-sized consumables, and can integrate the detection that originally required multiple operation chambers (sample pretreatment, mixing, reaction, separation, and detection chambers) onto a reagent disk, and automatically complete the entire analysis process on a small device. It has the advantages of short detection time and simultaneous detection of multiple indicators. Due to its inherent characteristics of being fast, sensitive, small, and portable, it can achieve "point-of-care testing" and has the potential to replace conventional chemical laboratories.

[0003] The common pre-packaging device for liquid reagents in a microfluidic reagent disk provided by the prior art injects the liquid reagent into a pre-packaged liquid capsule, then seals the pre-packaged liquid capsule with a sealing film, and then places the sealed pre-packaging device in a sample slot on the reagent disk (also known as a microfluidic chip). A puncturing end is provided at a corresponding position above the pre-packaging device on the reagent disk. During use, an upward force is applied below the reagent disk corresponding to the pre-packaging device to lift the pre-packaging device, and then the sealing film is punctured by the puncturing end above, and then the liquid reagent in the pre-packaged liquid capsule is completely released by the action of a centrifuge.

[0004] For example, Chinese Patent No. CN109967144A (hereinafter referred to as Document 1) discloses a dry chemical analyzer for detecting a microfluidic chip and a microfluidic chip. For a microfluidic chip using the liquid reagent pre-packaging device shown in Document 1, the whole process can be automated. Specifically, a lifting mechanism, a centrifugal mechanism, and a chip holding mechanism are used. The chip holding mechanism is provided with a chip fixing component for locking the microfluidic chip and a chip puncturing component for pressing the liquid reagent pre-packaging device in the sample slot of the microfluidic chip. The chip puncturing component is fixed on the chip fixing component, and the chip fixing component is connected to the centrifugal mechanism. The lifting mechanism drives the centrifugal mechanism to move up and down. Specifically, its specification records that "driven by the lifting motor 190, the lead screw 110 drives the transmission fixing seat 170 to rise, and the centrifugal mechanism 180 sleeved by the transmission fixing seat 170 rises accordingly. The chip holding mechanism fixed above the centrifugal mechanism 180 also rises and passes through the platform through hole or opening of the access platform 150 to contact the microfluidic chip 130. While the chip fixing component 160 locks the microfluidic chip to be tested, the chip puncturing component 162 lifts the liquid reagent pre-packaging device 131 in the sample slot of the microfluidic chip 130, so that the sealing film on the upper surface of the liquid reagent pre-packaging device 131 is punctured by the puncturing end of the microfluidic chip 130." Figure 1 ​

[0005] As can be seen from the above, the puncturing solution in Document 1 is consistent with the prior art principle of its attachment Figure 1 That is, the puncturing end is provided at the top of the pre-packaging device 131 and the sealing film. The pre-packaging device 131 is lifted by the chip puncturing component 162, so that the sealing film at the top of the pre-packaging device 131 contacts the upper puncturing end, and then the puncturing end punctures the sealing film. Then, under the action of the centrifuge, the liquid reagent in the pre-packaged liquid capsule is completely released.

[0006] However, the solution in Document 1 still has the following deficiencies: adding a motor and corresponding mechanisms (such as a lifting mechanism, a lead screw or a transfer motor, etc.) results in higher costs, and there is no automatic centering between its microfluidic chip (also known as a reagent tray) and the chip fixing component. Summary of the Invention

[0007] The purpose of the present invention is to provide a device for microfluidic detection in view of the deficiencies of the prior art, aiming to reduce the cost of the detection device while realizing the microfluidic detection function, and realizing the automatic centering of the reagent tray and its fixing component to improve the installation efficiency and convenience of the reagent tray.

[0008] The present invention realizes the above purpose through the following technical solutions: A device for microfluidic detection, comprising:

[0009] A dilution cup, having a plug post groove at the bottom, a liquid outlet hole communicating with the inner cavity of the dilution cup, and a pull ring film covering the liquid outlet hole through the notch of the plug post groove;

[0010] A reagent tray, having a cup placing cavity that is open at the top and on which the dilution cup is placed, a connecting portion at the bottom, the connecting portion being provided with a first magnetic attraction member, a guiding port communicating with the cup placing cavity, and an upper centering ratchet tooth; and

[0011] A fixing seat, having a guiding film tearing post matching the plug post groove, a second magnetic attraction member matching the first magnetic attraction member, and a lower centering ratchet tooth matching the upper centering ratchet tooth.

[0012] As a further solution of the present invention: The bottom of the dilution cup is further provided with a guiding film groove passing through the notch of the plug post groove, the liquid outlet hole is provided at the bottom of the guiding film groove and on one side of the plug post groove, and the pull ring film is attached to the bottom of the guiding film groove.

[0013] As a further solution of the present invention: the end of the guiding film tearing column is of an arc surface structure and protrudes from the top of the fixing base. The lower meshing tooth for centering and the second magnetic attracting member are arranged on the top of the fixing base. The microfluidic detection device further includes a centrifuge or a separating motor connected to the bottom of the fixing base. Specifically, the end of the guiding film tearing column is arranged to be of an arc surface structure, which is beneficial to applying a pushing and tearing force to the pull ring film, effectively reducing the possibility of the pull ring film being torn, and avoiding the situation that the part covering the liquid outlet hole cannot be completely torn open due to the pull ring film being torn or broken, resulting in the inability to open the liquid outlet hole. Furthermore, the failure rate is reduced.

[0014] As a further solution of the present invention: the first magnetic attracting member is replaced by an iron sheet, and the second magnetic attracting member is a magnet; or

[0015] the second magnetic attracting member is replaced by an iron sheet, and the first magnetic attracting member is a magnet.

[0016] As a further solution of the present invention: the lower meshing tooth for centering is replaced by a centering arc surface limiting convex, and the upper meshing tooth for centering is replaced by a centering limiting hole into which the centering arc surface limiting convex is inserted in a matching manner; or

[0017] the upper meshing tooth for centering is replaced by a centering arc surface limiting convex, and the lower meshing tooth for centering is replaced by a centering limiting hole into which the centering arc surface limiting convex is inserted in a matching manner. Specifically, under the automatic attraction of the first magnetic attracting member and the second magnetic attracting member, the centering arc surface limiting convex can be adaptively clamped into the limiting hole to achieve automatic centering. Furthermore, the entire installation process is simple and efficient, with few accessories and low manufacturing cost, effectively reducing the cost of the detection device. The automatic attraction of the first magnetic attracting member and the second magnetic attracting member, and the meshing of the upper meshing tooth for centering and the lower meshing tooth for centering achieve automatic centering, so that as long as the reagent tray is slightly pushed into the fixing base, the reagent tray can be automatically centered and the film tearing step can be carried out, effectively improving the installation efficiency and convenience.

[0018] As a further solution of the present invention: a guiding film rib is also arranged at the bottom of the guiding film groove and horizontally inserted between the inserting column groove and the liquid outlet hole.

[0019] As a further solution of the present invention: the outer side wall of the dilution cup is provided with a limiting clamping convex for circumferential limiting of the dilution cup, and a limiting wrapping point for central limiting of the dilution cup. The inner wall of the cup placing cavity near the open end is provided with a limiting clamping opening into which the limiting clamping convex is clamped;

[0020] the reagent tray is provided with a plurality of separation chambers, and a diversion slit communicating with the separation chambers and the cup placing cavity is also provided.

[0021] The present invention also provides another technical solution: a microfluidic detection method, including the following steps:

[0022] Liquid preparation step: Put the liquid reagent into the dilution cup, and then use the film sealing process to seal the mouth of the dilution cup;

[0023] First installation step: Install the dilution cup containing the liquid reagent in the cup placement cavity of the reagent tray. The plug column groove of the dilution cup faces and is adjacent to the guiding port of the cup placement cavity, and then use the covering member with a sample injection hole on the reagent tray to cover the top of the reagent tray;

[0024] Second installation step: Install the reagent tray with the dilution cup installed on the fixed seat. The guiding film tearing column of the fixed seat is inserted into the guiding port, the first magnetic attracting member and the second magnetic attracting member are attracted to each other, the upper meshing gear and the lower meshing gear are engaged, the guiding film tearing column abuts against the pull ring film and is inserted into the plug column groove, the pull ring film is torn open to open the liquid outlet hole at the bottom of the dilution cup, and the liquid reagent flows from the inner cavity of the dilution cup into the cup placement cavity through the liquid outlet hole;

[0025] Reagent separation step: Start the centrifuge or the separation motor to rotate the fixed seat, and the reagent tray and the dilution cup rotate synchronously, and the liquid reagent in the cup placement cavity is thrown out from the diversion slit and released into the corresponding separation chamber. Specifically, the covering member of the reagent tray can be replaced by a top cover or a glue film or a protective film or a heat-sealing film. The dilution cup is assembled in the reagent tray, and the position for pasting the pull ring film is designed at the bottom of the dilution cup, that is, the film guiding groove. The pull ring film is pasted on the film guiding groove, and one end far from the liquid outlet hole is the fixed end with a large adhesive force, and one end close to the liquid outlet hole is the liquid reagent outflow end of the dilution cup. A liquid outlet hole is designed at this end. Between the liquid outlet hole and the plug column groove, that is, a transverse convex strip, namely the film guiding rib, is designed near the center of the liquid outlet hole. Under the action of the film guiding rib, the guiding film tearing column presses against the pull ring film, converts the pressing force into a downward pulling force, and pulls open the pull ring film on the side of the liquid outlet hole, that is, the outflow end.

[0026] As a further solution of the present invention: The previous step of the liquid preparation step further includes:

[0027] Film pasting step: Paste the pull ring film on the bottom of the film guiding groove, so that the pull ring film passes through the notch of the plug column groove and adheres to the arc surface of the film guiding rib until it covers the liquid outlet hole at the bottom of the dilution cup;

[0028] The first installation step is: Install the dilution cup containing the liquid reagent in the cup placement cavity of the reagent tray. The plug column groove of the dilution cup faces and is adjacent to the guiding port of the cup placement cavity. Put the freeze-dried reagent balls into the reaction holes in the reagent tray, and then use the covering member with a sample injection hole on the reagent tray to cover the top of the reagent tray;

[0029] Between the first installation step and the second installation step, it further includes:

[0030] Pre-detection step: Add the sample material into the reagent tray from the sample injection hole of the covering member.

[0031] The beneficial effects of the present invention:

[0032] In this solution, by setting an insertion post groove, a liquid outlet hole and a pull-ring film at the bottom of the dilution cup, the pull-ring film covers the liquid outlet hole to prevent the liquid reagent in the inner cavity of the dilution cup from flowing out. The dilution cup is placed in the cup placement cavity of the reagent tray, and the insertion post groove at the bottom of the dilution cup faces and aligns with the guiding port at the bottom of the cup placement cavity. Then, the reagent tray is installed on the fixed seat. Specifically, the guiding port at the bottom of the cup placement cavity is aligned with the guiding film-tearing post on the fixed seat and inserted. When approaching a certain distance, the first magnetic attracting member and the second magnetic attracting member will automatically attract each other, and the upper meshing teeth and the lower meshing teeth will mesh with each other to achieve automatic centering, making it easier for the guiding film-tearing post to pass through the guiding port and be inserted exactly opposite to the insertion post groove at the bottom of the dilution cup. During this process, the guiding film-tearing post will abut against the pull-ring film at the bottom of the dilution cup, thereby applying a tearing force to the pull-ring film to tear it open, thus opening the liquid outlet hole at the bottom of the dilution cup, and enabling the liquid reagent to flow from the inner cavity of the dilution cup into the cup placement cavity of the reagent tray through the liquid outlet hole. Finally, the centrifuge or the separation motor is started to drive the fixed seat to rotate, and then the reagent tray and the dilution cup rotate synchronously to separate and release the liquid reagent in the reagent tray. The entire installation process is simple and efficient, with few accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic attraction of the first magnetic attracting member and the second magnetic attracting member, and the meshing of the upper meshing teeth and the lower meshing teeth to achieve automatic centering enable the reagent tray to be automatically centered and the film-tearing step to be carried out as long as the reagent tray is slightly pushed into the fixed seat, effectively improving the installation efficiency and convenience. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is a schematic structural diagram of the present invention.

[0034] Figure 2 is Figure 1 a schematic structural diagram from another perspective.

[0035] Figure 3 is Figure 1 a schematic exploded view of the structure.

[0036] Figure 4 is a schematic exploded sectional view of a structure of the present invention.

[0037] Figure 5 is Figure 4 the previous schematic exploded sectional view of the structure.

[0038] Figure 6 is Figure 5 the previous schematic exploded sectional view of the structure.

[0039] Figure 7 is a schematic structural diagram of a dilution cup according to the present invention.

[0040] Figure 8 is a schematic exploded view of a dilution cup according to the present invention.

[0041] Figure 9 This is another structural schematic diagram of the dilution cup of the present invention.

[0042] Figure 10 It is Figure 9 a structural schematic diagram from another perspective.

[0043] Figure 11 This is a structural schematic diagram of another embodiment of the present invention.

[0044] Figure 12 It is Figure 11 a structural schematic diagram from another perspective.

[0045] Reference numerals include:

[0046] 1 - dilution cup,

[0047] 11 - insertion post groove, 12 - film guiding groove, 13 - liquid outlet hole, 14 - film guiding rib, 15 - pull ring film, 16 - limiting clamping projection, 17 - heat-sealing film, 18 - limiting wrapping point;

[0048] 2 - reagent tray,

[0049] 21 - cup placement cavity, 22 - connecting part, 23 - first magnetic attraction part, 24 - separation chamber, 25 - diversion slit, 26 - covering part,

[0050] 211 - limiting bayonet,

[0051] 221 - guiding port, 222 - upper meshing tooth for alignment, 223 - alignment limiting hole,

[0052] 261 - sample injection hole;

[0053] 3 - fixing base,

[0054] 31 - guiding film tearing post, 32 - second magnetic attraction part, 33 - lower meshing tooth for alignment, 34 - alignment arc-shaped limiting projection. Detailed implementation manners

[0055] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention. It can be understood that the accompanying drawings are only for reference and illustration, and are not used to limit the present invention. The connection relationships shown in the drawings are only for clear description and do not limit the connection methods.

[0056] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention; the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance. In addition, unless otherwise clearly specified and defined, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0057] As Figures 1 to 10 shown, in an embodiment of the present invention, a device for microfluidic detection is provided, including: a dilution cup 1, a reagent tray 2, and a fixing base 3. Among them:

[0058] The dilution cup 1 is provided with a plug post groove 11 at the bottom, a liquid outlet hole 13 communicating with the inner cavity of the dilution cup 1, and a pull ring film 15 that passes through the notch of the plug post groove 11 and covers the liquid outlet hole 13;

[0059] The reagent tray 2 is provided with a cup placement cavity 21 that is open at the top and in which the dilution cup 1 is placed. The bottom is provided with a connection part 22. The connection part 22 is provided with a first magnetic attraction part 23, a guiding port 221 communicating with the cup placement cavity 21, and an upper meshing tooth 222 for alignment;

[0060] The fixing base 3 is provided with a guiding film tearing post 31 that matches the plug post groove 11, a second magnetic attraction part 32 that matches the first magnetic attraction part 23, and a lower meshing tooth 33 that matches the upper meshing tooth 222 for alignment. Specifically, the bottom of the fixing base 3 is used to connect to the rotating shaft of a centrifuge or a separation motor, and the guiding port 221 is used for the guiding film tearing post 31 to penetrate. After the dilution cup 1 is filled with the liquid to be detected, the open top of the dilution cup 1 can be sealed with a heat-sealing film 17. The pull ring film 15 can be a thin film.

[0061] In this solution, by providing a plug post groove 11, a liquid outlet hole 13 and a pull-ring film 15 at the bottom of the dilution cup 1, the pull-ring film 15 covers the liquid outlet hole 13 to prevent the liquid reagent in the inner cavity of the dilution cup 1 from flowing out. The dilution cup 1 is placed in the cup placement cavity 21 of the reagent tray 2, and the plug post groove 11 at the bottom of the dilution cup 1 faces and aligns with the guiding port 221 at the bottom of the cup placement cavity 21. Then, the reagent tray 2 is installed on the fixed seat 3. Specifically, the guiding port 221 at the bottom of the cup placement cavity 21 is aligned with the guiding film-tearing post 31 on the fixed seat 3 and inserted. When approaching a certain distance, the first magnetic attraction member 23 and the second magnetic attraction member 32 will automatically attract each other, and the upper meshing teeth 222 and the lower meshing teeth 33 are meshed to achieve automatic centering, making it easier for the guiding film-tearing post 31 to pass through the guiding port 221 and accurately insert into the plug post groove 11 at the bottom of the dilution cup 1. During this process, the guiding film-tearing post 31 will abut against the pull-ring film 15 at the bottom of the dilution cup 1, thereby applying a tearing force to the pull-ring film 15 to tear the pull-ring film 15, thus opening the liquid outlet hole 13 at the bottom of the dilution cup 1, and enabling the liquid reagent to flow from the inner cavity of the dilution cup 1 into the cup placement cavity 21 of the reagent tray 2 through the liquid outlet hole 13. Finally, the centrifuge or the separation motor is started to drive the fixed seat 3 to rotate, and then the reagent tray 2 and the dilution cup 1 rotate synchronously to separate and release the liquid reagent in the reagent tray 2. The entire installation process is simple and efficient, with few accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic attraction of the first magnetic attraction member 23 and the second magnetic attraction member 32, and the meshing of the upper meshing teeth 222 and the lower meshing teeth 33 to achieve automatic centering, enable the reagent tray 2 to be automatically centered and installed and the film-tearing step to be carried out as long as the reagent tray 2 is slightly pushed into the fixed seat 3, effectively improving the installation efficiency and convenience.

[0062] In one embodiment, as Figures 7 to 10 shown, a guiding film groove 12 is further provided at the bottom of the dilution cup 1, passing through the notch of the plug post groove 11. The liquid outlet hole 13 is provided at the bottom of the guiding film groove 12 and is located on one side of the plug post groove 11. The pull-ring film 15 is attached to the bottom of the guiding film groove 12. Specifically, the guiding film groove 12 adopts a strip-shaped groove structure. The pull-ring film 15 can be attached to the bottom of the guiding film groove 12 by double-sided tape. Furthermore, the entire installation process is simple and efficient, with few accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic attraction of the first magnetic attraction member 23 and the second magnetic attraction member 32, and the meshing of the upper meshing teeth 222 and the lower meshing teeth 33 to achieve automatic centering, enable the reagent tray 2 to be automatically centered and installed and the film-tearing step to be carried out as long as the reagent tray 2 is slightly pushed into the fixed seat 3, effectively improving the installation efficiency and convenience.

[0063] In another embodiment, as Figures 3 to 6As shown, the end of the guiding film tearing column 31 is an arc surface structure and protrudes from the top of the fixing base 3. The upper meshing lower rodent 33 and the second magnetic attracting member 32 are arranged on the top of the fixing base 3. This microfluidic detection device further includes a centrifuge or a separation motor connected to the bottom of the fixing base 3. Specifically, the end of the guiding film tearing column 31 is set as an arc surface structure, which is beneficial to applying a pushing and tearing force to the pull ring film 15, effectively reducing the possibility of the pull ring film 15 being torn, and avoiding the situation that the part covering the liquid outlet hole 13 cannot be completely torn open due to the pull ring film 15 being torn or broken, resulting in the inability to open the liquid outlet hole 13. Furthermore, the failure rate is reduced. Furthermore, the entire installation process is simple and efficient, with fewer accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic suction of the first magnetic attracting member 23 and the second magnetic attracting member 32, and the meshing of the upper meshing lower rodent 222 and the lower meshing lower rodent 33 achieve automatic centering, so that as long as the reagent tray 2 is slightly pushed into the fixing base 3, the reagent tray 2 can be automatically centered and installed and the film tearing step can be carried out, effectively improving the installation efficiency and convenience.

[0064] In yet another embodiment, as Figures 3 to 6 shown, the first magnetic attracting member 23 is replaced by an iron sheet, and the second magnetic attracting member 32 is a magnet; or

[0065] the second magnetic attracting member 32 is replaced by an iron sheet, and the first magnetic attracting member 23 is a magnet. Preferably, the first magnetic attracting member 23 is replaced by an iron sheet, and the iron sheet is embedded in the bottom of the reagent tray 2. Furthermore, the entire installation process is simple and efficient, with fewer accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic suction of the first magnetic attracting member 23 and the second magnetic attracting member 32, and the meshing of the upper meshing lower rodent 222 and the lower meshing lower rodent 33 achieve automatic centering, so that as long as the reagent tray 2 is slightly pushed into the fixing base 3, the reagent tray 2 can be automatically centered and installed and the film tearing step can be carried out, effectively improving the installation efficiency and convenience.

[0066] In still another embodiment, as Figure 11 and Figure 12 shown, the lower meshing lower rodent 33 is replaced by a centering arc surface limiting convex 34, and the upper meshing lower rodent 222 is replaced by a centering limiting hole 223 into which the centering arc surface limiting convex 34 is inserted; or

[0067] The upper and middle alignment ratchet 222 is replaced by the alignment arc surface limiting convex 34, and the lower alignment ratchet 33 is replaced by the alignment limiting hole 223 into which the alignment arc surface limiting convex 34 is inserted in a matching manner. Specifically, under the automatic attraction of the first magnetic attraction member 23 and the second magnetic attraction member 32, the alignment arc surface limiting convex 34 can be adaptively clamped into the limiting hole to achieve automatic alignment. Furthermore, the entire installation process is simple and efficient, with few accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic attraction of the first magnetic attraction member 23 and the second magnetic attraction member 32, and the engagement of the upper and middle alignment ratchet 222 and the lower alignment ratchet 33 achieve automatic alignment, so that as long as the reagent tray 2 is slightly pushed into the fixed seat 3, the automatic alignment installation of the reagent tray 2 and the film tearing step can be realized, effectively improving the installation efficiency and convenience.

[0068] In another embodiment, as Figures 7 to 10 shown, a film guiding rib 14 is also provided at the bottom of the film guiding groove 12 and horizontally inserted between the plug post groove 11 and the liquid outlet hole 13. Specifically, the film guiding rib 14 preferably has an arc surface structure. When the pull ring film 15 is attached to the bottom of the film guiding groove 12 and covers the liquid outlet hole 13, the film guiding rib 14 can slightly bulge the pull ring film 15 on the side close to the liquid outlet hole 13, preventing the pull ring film 15 from being stuck or torn when the pull ring film 15 is torn, making the pull ring film 15 easier to tear. Specifically, when the guiding film tearing post 31 is inserted into the plug post groove 11, under the action of the film guiding rib 14, the pull ring film 15 is easier to tear from one end of the liquid outlet hole 13, and it is easier to open the liquid outlet hole 13, avoiding the situation where the liquid outlet hole 13 is still sealed after the end far from the liquid outlet hole 13 is torn, greatly reducing the failure rate of the liquid reagent not flowing out due to the inability to open the liquid outlet hole 13. Furthermore, the entire installation process is simple and efficient, with few accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic attraction of the first magnetic attraction member 23 and the second magnetic attraction member 32, and the engagement of the upper and middle alignment ratchet 222 and the lower alignment ratchet 33 achieve automatic alignment, so that as long as the reagent tray 2 is slightly pushed into the fixed seat 3, the automatic alignment installation of the reagent tray 2 and the film tearing step can be realized, effectively improving the installation efficiency and convenience.

[0069] In another embodiment, as Figure 3 and Figure 4As shown in the figure, a limiting clamping projection 16 for circumferential limiting of the dilution cup 1 is provided on the outer side wall of the dilution cup 1, and a limiting bump point 18 for central limiting of the dilution cup 1 is also provided. One end of the inner wall of the cup placement cavity 21 close to the open end is provided with a limiting bayonet 211 into which the limiting clamping projection 16 is snapped. Specifically, the limiting clamping projection 16 enables the dilution cup 1 to move synchronously with the reagent tray 2, avoiding relative movement between the two. In order to ensure that there is enough clearance between the outer wall of the dilution cup 1 and the inner wall of the cup placement cavity 21 of the reagent tray 2, so that the liquid reagent in the dilution cup 1 can flow from this clearance to the diversion seam 25 and then to the separation chamber 24. Therefore, the diameter of the dilution cup 1 needs to be smaller than the diameter of the cup placement cavity 21 of the reagent tray 2. After adding the limiting bump point 18 to the outer side wall of the dilution cup 1, it can be ensured that the dilution cup 1 can always abut against the inner side wall of the cup placement cavity 21, thereby ensuring that the dilution cup 1 will not be eccentric during rotation. Among them, multiple limiting bump points 18 are provided, preferably 3. The material of the dilution cup 1 is plastic, and the limiting bump point 18 and the dilution cup 1 are integrally formed.

[0070] The reagent tray 2 is provided with a number of separation chambers 24, and a diversion seam 25 communicating with the separation chamber 24 and the cup placement cavity 21 is also provided. Specifically, the diversion seam 25 is provided on the edge of the open end of the cup placement cavity 21. Furthermore, the entire installation process is simple and efficient, with few accessories and low manufacturing cost, effectively reducing the cost of the detection device. The automatic suction of the first magnetic part 23 and the second magnetic part 32, and the meshing of the upper meshing tooth 222 and the lower meshing tooth 33 for centering realize automatic centering, so that as long as the reagent tray 2 is slightly pushed into the fixed seat 3, the reagent tray 2 can be automatically centered and installed and the film tearing step can be carried out, effectively improving the installation efficiency and convenience.

[0071] A microfluidic detection method, as Figures 4 to 6 shown, includes: a liquid preparation step, a first installation step, a second installation step, and a reagent separation step. Among them:

[0072] Liquid preparation step: Put a liquid reagent (such as a diluent) into the dilution cup 1, and then seal the cup mouth of the dilution cup 1 by using a film sealing process. Optionally, the cup mouth of the dilution cup 1 can be heat-sealed with a heat-sealing film 17.

[0073] First installation step: Install the dilution cup 1 containing the liquid reagent into the cup placement cavity 21 of the reagent tray 2. The plug column groove 11 of the dilution cup 1 is opposite to and adjacent to the guiding port 221 of the cup placement cavity 21, and then cover the top of the reagent tray 2 with a covering member 26 provided with a sample injection hole 261 on the reagent tray 2. Among them, the covering member 26 can be a top cover or a film.

[0074] Second installation step: Install the reagent tray 2 with the dilution cup 1 on the fixed seat 3. The guiding film-tearing column 31 of the fixed seat 3 is inserted into the guiding port 221. The first magnetic part 23 and the second magnetic part 32 are magnetically attracted to each other. The upper meshing tooth 222 is aligned with the lower meshing tooth 33 and engaged. The guiding film-tearing column 31 abuts against the pull-ring film 15 and is inserted into the plug column groove 11. The pull-ring film 15 is torn open to open the liquid outlet hole 13 at the bottom of the dilution cup 1. The liquid reagent flows from the inner cavity of the dilution cup 1 into the cup placement cavity 21 through the liquid outlet hole 13.

[0075] Reagent separation step: Start the centrifuge or the separation motor to rotate the fixed seat 3. The reagent tray 2 and the dilution cup 1 rotate synchronously. The liquid reagent in the cup placement cavity 21 is thrown out from the diversion slit 25 and released into the corresponding separation chamber 24. Specifically, the covering member 26 of the reagent tray 2 can be replaced by a top cover, a film, a protective film, or a heat-sealing film 17. The dilution cup 1 is assembled in the reagent tray 2. A position for pasting the pull-ring film 15, that is, the film guiding groove 12, is designed at the bottom of the dilution cup 1. The pull-ring film 15 is pasted on the film guiding groove 12. One end far from the liquid outlet hole 13 is the fixed end with a large adhesive force, and one end close to the liquid outlet hole 13 is the liquid reagent outflow end of the dilution cup 1. A liquid outlet hole 13 is designed at this end. Between the liquid outlet hole 13 and the plug column groove 11, that is, a transverse convex strip, namely the film guiding rib 14, is designed at the center of the liquid outlet hole 13. Under the action of the film guiding rib 14, the guiding film-tearing column 31 presses against the pull-ring film 15, converts the pressing force into a downward pulling force, and pulls open the pull-ring film 15 on the outflow end side of the liquid outlet hole 13. Furthermore, the entire installation process is simple and efficient, with few accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic magnetic attraction of the first magnetic part 23 and the second magnetic part 32, and the meshing of the upper meshing tooth 222 and the lower meshing tooth 33 achieve automatic centering. As long as the reagent tray 2 is slightly pushed into the fixed seat 3, the reagent tray 2 can be automatically centered and installed, and the film-tearing step can be carried out, effectively improving the installation efficiency and convenience.

[0076] In another embodiment, as Figures 7 to 10 shown, the previous step of the stock solution preparation step further includes:

[0077] Film pasting step: Paste the pull-ring film 15 on the bottom of the film guiding groove 12, so that the pull-ring film 15 passes through the notch of the plug column groove 11 and adheres to the arc surface of the film guiding rib 14 until it covers the liquid outlet hole 13 at the bottom of the dilution cup 1.

[0078] The first installation step is: Install the dilution cup 1 containing the liquid reagent in the cup placement cavity 21 of the reagent tray 2. The plug column groove 11 of the dilution cup 1 is opposite to and adjacent to the guiding port 221 of the cup placement cavity 21. The reaction holes in the reagent tray 2 are filled with freeze-dried reagent pellets, and then the covering member 26 provided with the sample injection hole 261 of the reagent tray 2 is used to cover the top of the reagent tray 2.

[0079] Between the first installation step and the second installation step, there is also included:

[0080] Pre - detection steps: Add the sample material (such as blood) into the reagent tray 2 from the sample injection hole 261 of the covering member 26. During the test, inject the blood into the sample injection hole 261 to enter the reagent tray 2. Then, the blood and the liquid reagent in the dilution cup 1 are mixed during the centrifugation process, that is, when centrifuging, the liquid reagent in the dilution cup 1 is separated into the quantitative chamber of the reagent tray 2. Furthermore, the entire installation process is simple and efficient, with fewer accessories and low manufacturing costs, effectively reducing the cost of the detection device. The automatic suction of the first magnetic member 23 and the second magnetic member 32, and the meshing of the upper alignment ratchet 222 and the lower alignment ratchet 33 achieve automatic centering, so that as long as the reagent tray 2 is slightly pushed into the fixed seat 3, the automatic centering installation of the reagent tray 2 and the film - tearing step can be realized, effectively improving the installation efficiency and convenience.

[0081] In addition, through the mutual meshing between the lower alignment ratchet 33 at the top of the fixed seat 3 and the upper alignment ratchet 222 at the bottom of the reagent tray 2, the circumferential limit and central alignment of the reagent tray 2 are achieved; through the adsorption of the second magnetic member 32, that is, the magnet, the pull - ring film at the bottom of the dilution cup 1 is automatically torn. Compared with the method in Document 1 that uses the operation of a motor to lift or the motor and cam structure to eject the pre - encapsulated device, so that the sealing film on the upper surface of the pre - encapsulated device is punctured by the puncturing end above, the cost of this solution is lower and the structure is simpler.

[0082] In addition, it should be mentioned that, in addition, this solution realizes the tearing of the pull - ring film 15 from below the dilution cup 1. Compared with Document 1 where the sealing film on the upper surface of the pre - encapsulated device contacts the puncturing end above, there are the following advantages in tearing the pull - ring film 15 from the bottom of the dilution cup 1:

[0083] Avoiding contamination: Tearing the pull - ring film 15 from the bottom of the dilution cup 1 can generally reduce the risk of contamination of the liquid reagent by the external environment. In contrast, puncturing the sealing film in Document 1 may introduce additional contaminants, which is particularly important for some particularly sensitive detection systems, such as high - precision experiments in the laboratory or medical diagnosis;

[0084] Ease of operation: Tearing the pull - ring film 15 is generally easier to operate without additional tools or special skills. This can reduce the possibility of operation errors in actual use, improve work efficiency and reliability, and effectively reduce the situation where the liquid outlet hole 13 cannot be opened;

[0085] Maintaining the stability of the liquid: Tearing the pull - ring film 15 can reduce the impact on the structure or properties of the liquid itself, maintaining its stability and original characteristics. In contrast, puncturing the sealing film in Document 1 may introduce air bubbles or other unnecessary reactions inside the liquid to be tested, affecting the stability and accuracy of the liquid;

[0086] Safer: Tearing the pull-ring film 15 will not produce sharp debris, reducing the risk of stabbing;

[0087] More environmentally friendly: The waste generated by tearing the pull-ring film 15 is easier to handle, avoiding the sharp fragments and unsealed film fragments that may be generated by piercing the sealing film in Document 1;

[0088] Easier to control: Tearing the pull-ring film 15 enables the liquid reagent to flow out from the liquid outlet hole 13 at the bottom of the dilution cup 1, and the release speed and amount of the liquid reagent are relatively stable, avoiding the splashing or overflow of the liquid to be measured caused by the piercing solution.

[0089] In the description and claims of this application, the words "comprising / including" and the words "having / including" and their variants are used to specify the presence of the stated features, values, steps or components, but do not exclude the presence or addition of one or more other features, values, steps, components or combinations thereof.

[0090] Some features of the present invention are described separately in different embodiments for clarity. However, these features can also be combined and described in a single embodiment. On the contrary, some features of the present invention are described only in a single embodiment for brevity. However, these features can also be described separately or in any suitable combination in different embodiments.

[0091] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microfluidic detection device, characterized in that: include: A dilution cup (1) having a column insertion groove (11) at the bottom, a liquid outlet hole (13) connected to the inner cavity of the dilution cup (1), and a ring-pull film (15) passing through the notch of the column insertion groove (11) and sealing the liquid outlet hole (13); A reagent disk (2) is provided with a cup placement cavity (21) which is open toward the top and in which the dilution cup (1) is placed, and a connecting portion (22) is provided at the bottom, wherein the connecting portion (22) is provided with a first magnetic attraction member (23), a guide port (221) connected to the cup placement cavity (21), and a centering upper tooth (222); and The fixing seat (3) is provided with a film-tearing guide column (31) matching the column insertion slot (11), a second magnetic attraction component (32) matching the first magnetic attraction component (23), and a centering lower tooth (33) matching the centering upper tooth (222).

2. The microfluidic detection device according to claim 1, characterized in that: The bottom of the dilution cup (1) is also provided with a film guide groove (12) passing through the notch of the column insertion groove (11); the liquid outlet hole (13) is provided at the bottom of the film guide groove (12) and is located on one side of the column insertion groove (11); and the pull ring film (15) is attached to the bottom of the film guide groove (12).

3. The microfluidic detection device according to claim 1, characterized in that: The end of the film-tearing guide column (31) is a curved surface structure and extends out of the top of the fixed seat (3). The centering lower teeth (33) and the second magnetic attraction member (32) are arranged on the top of the fixed seat (3). The microfluidic detection device also includes a centrifuge or a separation motor connected to the bottom of the fixed seat (3).

4. The microfluidic detection device according to claim 1, characterized in that: The first magnetic attraction member (23) is replaced by an iron sheet, and the second magnetic attraction member (32) is a magnet; or The second magnetic attraction member (32) is replaced by an iron sheet, and the first magnetic attraction member (23) is a magnet.

5. The microfluidic detection device according to claim 1, characterized in that: The centering lower tooth (33) is replaced by a centering arc-surface limiting protrusion (34), and the centering upper tooth (222) is replaced by a centering limiting hole (223) that matches and is inserted into the centering arc-surface limiting protrusion (34); or The centering upper tooth (222) is replaced by a centering arc surface limiting protrusion (34), and the centering lower tooth (33) is replaced by a centering limiting hole (223) that is matched and inserted into the centering arc surface limiting protrusion (34).

6. The microfluidic detection device according to claim 2, characterized in that: The bottom of the film guiding groove (12) is also provided with a film guiding rib (14) which is inserted transversely between the column inserting groove (11) and the liquid outlet hole (13).

7. The microfluidic detection device according to claim 1, characterized in that: The outer wall of the dilution cup (1) is provided with a limiting clamping protrusion (16) for limiting the circumferential position of the dilution cup (1), and is also provided with a limiting point (18) for limiting the central position of the dilution cup (1); and the inner wall of the cup placement cavity (21) is provided with a limiting clamping notch (211) which is clamped by the limiting clamping protrusion (16); The reagent disk (2) is provided with a plurality of separation chambers (24), and is also provided with a guide slit (25) communicating between the separation chambers (24) and the cup placement cavity (21).

Images