Double-sleeve continuous flushing negative pressure aspiration liquid culture medium set

Abstract

The utility model discloses a double-sleeve continuous flushing negative pressure aspiration liquid culture medium set, which comprises an aerobic culture bottle, an anaerobic culture bottle and a culture bottle placing box, the aerobic culture bottle and the anaerobic culture bottle are arranged in the culture bottle placing box, an air cleaning component and an injection component are arranged in the anaerobic culture bottle, drainage liquid is stored in the injection component, and the drainage liquid is filled in the culture bottle placing box. And the air removal assembly exhausts air in the drainage liquid in the injection assembly. The device has the advantages that culture flasks containing aerobic and anaerobic culture solutions are stored in the culture flask placement boxes, the culture flasks can be opened for use during sampling, the negative pressure connector and the liquid inlet connector are directly connected with the negative pressure tube and the double sleeves, negative pressure sampling of drainage liquid is achieved, operation can be achieved by a single person, medical staff are prevented from making contact with the drainage liquid, and the air removal assembly is used for removing air from the drainage liquid. The air in the drainage liquid in the injection tube is discharged, and then the drainage liquid with the air removed is injected into the anaerobic culture bottle through the injection assembly, so that the air is prevented from entering the anaerobic culture liquid, and the accuracy of a culture result is ensured.

Description

Technical Field

[0001] The utility model specifically relates to a double-tube continuous flushing negative pressure suction liquid culture medium set. Background Art

[0002] Infectious pancrealic necrosis (IPN) is a deep soft tissue abscess, formerly known as pancreatic abscess. Currently, with the continuous release of medical evidence, the minimally invasive "step-up" approach has become the preferred treatment strategy for IPN. Double-cannula continuous flushing and negative pressure aspiration is the second step of the "step-up" approach. IPN patients often have repeated high fever clinically. While taking cooling and fever reduction measures, it is necessary to find the cause of the fever. Clinically, blood culture and drainage fluid culture (aerobic and anaerobic) are often used to identify pathogenic bacteria. For the drug resistance of the bacteria cultured from the blood and drainage fluid, targeted antibiotic treatment is used, which is particularly important for controlling the patient's condition.

[0003] The front end of the double cannula is usually placed in one or more abscess cavities, and the tube is exposed for a long time. When the double cannula needs to be retained for drainage fluid culture, the following two methods are commonly used clinically:

[0004] 1. Use a syringe to directly draw out liquid from the end of the tube. However, due to pressure problems, it is difficult to draw out the liquid or the amount retained is far from meeting the standard. Repeated forceful drawing not only causes local discomfort to the patient but is also prone to contamination.

[0005] 2. The pus drawn out by negative pressure suction during continuous flushing with double cannulas will be stored in a bag. When taking specimens, the nurse will assist the doctor in pausing flushing, separating the pipeline from the inner cyst of the suction bottle, and pouring the solution out from the entrance of the inner cyst. Two people must cooperate and pour it into a sterile cup. Then, pus is drawn from the sterile cup and injected into the blood oxygen and anaerobic blood culture bottles respectively. After the specimen is taken, the inner cyst is stuffed back into the drainage bottle or directly replaced with a new one. In this process, the operator may be splashed with pus if he is not careful, causing physical and mental discomfort. It also wastes time, human resources, and invisibly increases the cost of patients. In addition, the solution poured out from the inner cyst is kept outside for a long time, and secondary contamination cannot be ruled out.

[0006] In the Chinese patent: 201620258972.5, the name is: A VSD negative pressure suction device capable of instant sampling and detection, which records that: the drainage channel includes at least one double-set pipeline, the double-set pipeline has a drainage section and a negative pressure section; a plurality of drainage holes are opened on the wall of the drainage section, the VSD dressing wraps the drainage section, and the negative pressure section is connected to a negative pressure source;

[0007] At least one sampling tee is provided near the negative pressure section of the double - sleeve pipe. One end of the sampling tee is connected to the drainage section, one end is connected to the negative pressure section, and the other end is connected to a sampling interface; the sampling interface is hermetically connected to a color - developing culture dish and / or a liquid storage bottle.

[0008] As shown in the attached drawings of the patent: 201620258972.5, this negative - pressure suction device mainly uses a negative - pressure drainage device that can instantaneously sample and detect to instantaneously detect the physiological parameters of wound secretions. This device mainly uses a three - way valve to control the suction of the drainage fluid. When the drainage fluid is suctioned into the culture dish, oxygen will enter the culture dish along with it. Therefore, this structure is only applicable to aerobic culture media and is not applicable to anaerobic culture media. Summary of the Invention

[0009] The technical problem to be solved by the present utility model is that in the background technology, it is inconvenient to collect liquid samples of the drainage fluid in the existing double - sleeve pipe for continuous flushing and negative - pressure suction, and when sampling the drainage fluid, external air easily enters the anaerobic culture medium, affecting the culture results.

[0010] In view of the above - mentioned technical problem, a set of double - sleeve pipe for continuous flushing and negative - pressure suction liquid culture medium is proposed; it is realized through the following technical solutions: A set of double - sleeve pipe for continuous flushing and negative - pressure suction liquid culture medium includes an aerobic culture bottle, an anaerobic culture bottle, and a culture - bottle placement box. The aerobic culture bottle for storing aerobic culture medium is detachably arranged in the culture - bottle placement box, and the anaerobic culture bottle for storing anaerobic culture medium is detachably arranged in the culture - bottle placement box;

[0011] The aerobic culture bottle includes a bottle body and an aerobic bottle cap. The aerobic bottle cap is detachably connected to the bottle body. The aerobic bottle cap includes a negative - pressure joint and a liquid - inlet joint. The negative - pressure joint and the liquid - inlet joint are controllably sealed by a sealing cover. The negative - pressure joint is connected to an external negative - pressure pipe, and the liquid - inlet joint is connected to an external double - sleeve pipe. The liquid in the double - sleeve pipe flows into the bottle body through the liquid - inlet joint;

[0012] The anaerobic culture bottle includes a bottle body and an anaerobic bottle cap. The anaerobic bottle cap is detachably connected to the bottle body. The anaerobic bottle cap includes an air - scavenging component and an injection component. The injection component is connected to the liquid - inlet joint on the anaerobic bottle cap, and the air - scavenging component is connected to the negative - pressure joint on the anaerobic bottle cap. The air - scavenging component is communicated with the injection component. The air - scavenging component discharges the air sucked into the injection component, and the injection component injects the air - free suction liquid into the bottle body storing the anaerobic bacteria culture medium.

[0013] For the optimization of the technical solution of the present utility model, an elastic sealing assembly is provided on the aerobic bottle cap and the anaerobic bottle cap. The elastic sealing assembly includes an installation rod, a sealing cone, and a first spring. The sealing cone is inserted into the installation rod. The installation rod is arranged inside the negative pressure connector and the liquid inlet connector. The first spring is sleeved on the sealing cone. The sealing cone can move controllably along the installation rod to control the opening and closing of the negative pressure connector and the liquid inlet connector. The setting of the elastic sealing assembly enables the elastic sealing assembly to seal the negative pressure connector and the liquid inlet connector when the negative pressure tube and the double-tube are not connected, preventing external air from entering the bottle body, ensuring the sealing performance of the culture bottle, and preventing secondary contamination.

[0014] For the optimization of the technical solution of the present utility model, the elastic sealing assembly is provided on both the negative pressure connector and the liquid inlet connector of the aerobic bottle cap and the anaerobic bottle cap. Among them, the sealing cone of the elastic sealing assembly arranged inside the negative pressure connector closes the upper end of the negative pressure connector, and the sealing cone of the elastic sealing assembly inside the liquid inlet connector closes the lower end of the liquid inlet connector. Such a setting enables the elastic sealing assembly to open only during negative pressure suction, sucking the drainage fluid into the culture bottle.

[0015] For the optimization of the technical solution of the present utility model, the injection assembly includes an injection tube and an injection push rod. The injection push rod is inserted into the injection tube. The injection tube is arranged on the anaerobic bottle cap, and the injection push rod passes through the anaerobic bottle cap and exposes outside the anaerobic bottle cap. The setting of the injection assembly facilitates the temporary storage of the drainage fluid and also facilitates the injection of the air-free drainage fluid into the anaerobic culture bottle.

[0016] For the optimization of the technical solution of the present utility model, a liquid inlet is provided on the side wall of the injection tube, and a liquid inlet tube is arranged on the liquid inlet. One end of the liquid inlet tube is connected to the liquid inlet, and the other end is connected to the liquid inlet connector on the anaerobic bottle cap. The setting of the liquid inlet tube facilitates guiding the drainage fluid into the injection tube.

[0017] For the optimization of the technical solution of the present utility model, a first exhaust port is provided on the side wall of the injection tube. The first exhaust port is communicated with the air removal assembly. A second exhaust port is provided on the injection piston of the injection push rod. The second exhaust port is communicated with the first exhaust port, and the second exhaust port can be controllably opened and closed through an exhaust port sealing rod installed on the injection push rod. The setting of the second exhaust port facilitates the discharge of the air in the drainage fluid and also facilitates guiding the overflowing drainage fluid into the overflow bin, ensuring the complete discharge of the air in the drainage fluid.

[0018] For the optimization of the technical solution of the present utility model, the air removal assembly includes an overflow bin, a functional bin, and an exhaust pipe. The overflow bin is sleeved outside the injection tube. The functional bin is connected to the overflow bin. An exhaust port communicated with the first exhaust port is provided on the functional bin. An exhaust pipe is provided on the functional bin, and the exhaust pipe is connected to the negative pressure connector on the anaerobic bottle cap. The setting of the air removal assembly facilitates the discharge of the air in the drainage fluid, making the drainage fluid injected into the anaerobic culture bottle air-free, ensuring the accuracy of the detection.

[0019] For the optimization of the technical solution of the present utility model, the bottle body is made of a transparent material, and a scale for observing the quantity of the liquid inside the bottle body is provided on the side wall of the bottle body. Such a setting facilitates observing the quantity of the drainage fluid in the culture bottle and is convenient to use.

[0020] For the optimization of the technical solution of the present utility model, the culture bottle placement box includes a partition board and an observation window. The partition board is arranged inside the culture bottle placement box and divides the culture bottle placement box into two culture bottle placement cavities. The observation windows are opened on both sides of the culture bottle placement box, and the quantity of the liquid inside the bottle body is observed through the observation windows.

[0021] The beneficial effects of the present utility model compared with the prior art are as follows:

[0022] In the technical solution of the present utility model, the culture bottle placement box is used to store the culture bottles containing two kinds of culture media, namely aerobic and anaerobic culture media. Two culture bottles are combined and placed in one box. When sampling, they can be directly opened and used respectively, meeting the clinical needs, with simple operation. Also, the negative pressure connector and the liquid inlet connector are directly connected to the negative pressure tube and the double - sleeve tube respectively to realize the negative pressure sampling of the drainage fluid, which can be completed in one extraction, avoiding repeated extraction. It can be operated by a single person, avoiding the contact between medical staff and the drainage fluid, preventing occupational exposure and secondary pollution at the same time. Moreover, the air removal component arranged in the anaerobic culture bottle is used to remove the air in the drainage fluid sucked into the syringe, and then the injection component is used to inject the drainage fluid with the air removed into the anaerobic culture bottle, preventing oxygen from entering the anaerobic culture medium and ensuring the accuracy of the culture result. Description of the Drawings

[0023] Figure 1 is a three - dimensional schematic diagram of the present utility model;

[0024] Figure 2 is a three - dimensional schematic diagram of the aerobic culture bottle;

[0025] Figure 3 is an exploded view of the aerobic culture bottle;

[0026] Figure 4 is a partial cross - sectional view of the aerobic culture bottle;

[0027] Figure 5 is Figure 4 the enlarged view at A in

[0028] Figure 6 is a three - dimensional schematic diagram of the anaerobic culture bottle;

[0029] Figure 7 is a three - dimensional schematic diagram of the air removal component and the injection component;

[0030] Figure 8 is a partial cross - sectional view of the air removal component and the injection component;

[0031] Figure 9 is Figure 8 The enlarged view at position B in

[0032] Figure 10 is Figure 8 The enlarged view at position C in

[0033] Figure 11 is the three-dimensional schematic diagram of the push rod limit component;

[0034] Figure 12 is the partial sectional view of the anaerobic culture bottle;

[0035] Figure 13 is the three-dimensional schematic diagram of the culture bottle placement box;

[0036] Explanation of reference numerals: 1 - aerobic culture bottle, 11 - bottle body, 12 - aerobic bottle cap, 13 - scale, 14 - hand support, 15 - screw joint, 16 - negative pressure joint, 17 - liquid inlet joint, 18 - sealing gasket, 19 - sealing cover, 2 - elastic sealing component, 21 - mounting rod, 22 - sealing cone, 23 - connecting rod, 24 - first spring, 25 - spring mounting block, 3 - anaerobic culture bottle, 31 - anaerobic bottle cap, 32 - functional hole, 4 - air scavenging component, 41 - overflow chamber, 42 - functional chamber, 43 - exhaust pipe, 5 - injection component, 501 - injection tube, 502 - injection push rod, 503 - exhaust port sealing rod, 504 - liquid inlet, 505 - injection port, 506 - first exhaust port, 507 - injection piston, 508 - second exhaust port, 509 - sealing head, 510 - second spring, 511 - limit ring, 512 - liquid inlet pipe, 513 - sealing piece, 6 - culture bottle placement box, 61 - partition board, 62 - observation window, 63 - culture bottle placement cavity, 7 - push rod limit component, 71 - limit rod, 72 - limit groove, 73 - limit clamp. Detailed implementation manners

[0037] Next, the technical solutions in the embodiments of the present utility model will be described in detail in conjunction with the attached Figure 1-13 drawings in the embodiments of the present utility model. Embodiment

[0038] As Figure 1 shown, a double - sleeve continuous flushing negative - pressure suction liquid culture medium set includes an aerobic culture bottle 1, an anaerobic culture bottle 3, and a culture bottle placement box 6. The aerobic culture bottle 1 for storing aerobic culture solution is placed in the culture bottle placement box 6, and the anaerobic culture bottle 3 for storing anaerobic culture solution is placed in the culture bottle placement box 6.

[0039] The main function of the aerobic culture bottle 1 is to store the aerobic culture medium. At the same time, a negative pressure connector 16 and a liquid inlet connector 17 are also provided on the aerobic culture bottle 1. The negative pressure connector 16 is connected to an external negative pressure tube, and the liquid inlet connector 17 is connected to a double cannula on the patient. Through the negative pressure tube, the drainage fluid in the double cannula can be attracted into the aerobic culture bottle 1 for culturing the drainage fluid.

[0040] The main function of the anaerobic culture bottle 3 is to store the anaerobic culture medium. At the same time, a negative pressure connector 16 and a liquid inlet connector 17 are also provided on the anaerobic culture bottle 3. The negative pressure connector 16 is connected to an external negative pressure tube, and the liquid inlet connector 17 is connected to a double cannula on the patient. An air removal component 4 and an injection component 5 are also provided in the anaerobic culture bottle 3. The air removal component 4 and the injection component 5 are connected, and the negative pressure connector 16 and the liquid inlet connector 17 are communicated with the air removal component 4 and the injection component 5.

[0041] Through the negative pressure tube, the drainage fluid in the double cannula can be attracted into the injection component 5, and the air removal component 4 can discharge the air carried when attracting the drainage fluid, so that only the drainage fluid remains in the injection tube 501 of the injection component 5, without air. The injection component 5 injects the drainage fluid after removing air into the anaerobic culture bottle 3 for culturing the drainage fluid.

[0042] The main function of the culture bottle placement box 6 is to serve as a carrier for storing the aerobic culture bottle 1 and the anaerobic culture bottle 3, facilitating the placement of the culture bottles, and also facilitating the medical staff to take and sample the drainage fluid.

[0043] As Figure 1 、 2 As shown in Figures 1 and 3, the aerobic culture bottle 1 includes a bottle body 11, an aerobic bottle cap 12 and an elastic sealing component 2. The aerobic bottle cap 12 is detachably screwed onto the bottle body 11. The elastic sealing component 2 is provided on the aerobic bottle cap 12 to controllably open and close the opening on the aerobic bottle cap 12.

[0044] The bottle body 11 is a hollow rigid plastic bottle with a rectangular cross-section. In this embodiment, it is preferably made of PP plastic. In addition, for easy observation, the bottle body 11 is transparent. For easy installation of the aerobic bottle cap 12, a circular hole is opened on the upper end surface of the bottle body 11, and a thread is provided on the inner side wall of this circular hole. The through hole with the thread is named the spiral interface 15. The aerobic bottle cap 12 is screwed onto the bottle body 11 through the spiral interface 15.

[0045] In order to facilitate observing the amount of the attracted drainage fluid in the bottle body 11 of the aerobic culture bottle 1, a scale 13 is also printed on the outer side wall of the bottle body 11. Through the scale 13, it is convenient to check the amount of the drainage fluid attracted into the bottle body 11.

[0046] To facilitate the user's grip on the bottle body 11, on one of the sides of the bottle body 11 where no scale 13 is printed, a handrest 14 is fixedly installed perpendicular to the side. The handrest 14 is made of flexible plastic, semi-circular when unfolded and sheet-shaped when folded, and adheres to the side wall of the bottle body 11. When sampling, the operator reversely inserts the hand into the handrest 14 (like putting on a ring), which can play a fixing role and facilitate the aspiration operation.

[0047] The aerobic bottle cap 12 is a plastic cap with a rectangular cross-section. On the end face of the aerobic bottle cap 12, an annular tube protrudes perpendicularly to the end face. Threads are provided on the surface of the annular tube, and through the threads, the aerobic bottle cap 12 can be screwed onto the spiral interface 15 of the bottle body 11, facilitating the perfusion of the aerobic culture medium into the bottle body 11 in the early stage and also facilitating the sampling and detection of the culture medium in the later stage of cultivation, which is convenient to use.

[0048] To ensure the sealing between the aerobic bottle cap 12 and the bottle body 11, a sealing gasket 18 is placed on the end face of the aerobic bottle cap 12. The sealing gasket 18 is placed between the aerobic bottle cap 12 and the bottle body 11. When the bottle body 11 and the aerobic bottle cap 12 are tightened, the sealing performance between the two can be improved through the sealing gasket 18.

[0049] To facilitate the drainage fluid to enter the aerobic culture bottle 1, a negative pressure connector 16 and a liquid inlet connector 17 are provided on the upper end face of the aerobic bottle cap 12. Both the negative pressure connector 16 and the liquid inlet connector 17 are circular tubular connectors, fixedly connected to the aerobic bottle cap 12 and communicating with the bottle body 11.

[0050] External threads are provided on the outer side walls of the negative pressure connector 16 and the liquid inlet connector 17. Through the threads, the negative pressure tube connecting the outside and the negative pressure gauge can be screwed onto the negative pressure connector 16 through a threaded joint, and at the same time, the end of the double catheter connected to the patient can be connected to the liquid inlet connector 17 through a threaded joint.

[0051] The negative pressure tube and the negative pressure gauge are existing devices. The main function of the negative pressure gauge is to generate negative pressure. Through the negative pressure gauge, a negative pressure suction force can be generated on the aerobic culture bottle 1, and then the drainage fluid can be attracted into the aerobic culture bottle 1 through the double catheter and the liquid inlet connector 17, which is convenient to use.

[0052] When not in use, to prevent external dust from falling into the aerobic culture bottle 1 and contaminating the culture medium, caps 19 are also screwed onto the negative pressure connector 16 and the liquid inlet connector 17. The caps 19 are connected to the negative pressure connector 16 or the liquid inlet connector 17 through a traction rope. When in use, unscrew the caps 19, and after use, screw the caps 19 back on to prevent external dust and debris from falling into the aerobic culture bottle 1.

[0053] Such as Figure 3 、 4As shown in FIGS. 4 and 5, in order to prevent the culture medium in the bottle body 11 from leaking from the negative pressure connector 16 and the liquid inlet connector 17 due to shaking during transportation, and at the same time to prevent dust from falling into the negative pressure connector 16 and the liquid inlet connector 17 when the sealing cover 19 is opened and the negative pressure tube or the double tube is not connected, and in order to facilitate further restriction on the opening and closing of the negative pressure connector 16 and the liquid inlet connector 17, an elastic sealing assembly 2 is provided in the negative pressure connector 16 and the liquid inlet connector 17, and the elastic sealing assembly 2 controls the opening and closing of the negative pressure connector 16 and the liquid inlet connector 17.

[0054] The elastic sealing assembly 2 includes a mounting rod 21, a sealing cone 22 and a first spring 24. The mounting rod 21 is a plastic rod with a rectangular cross-section. The mounting rod 21 is fixed on the inner wall of the negative pressure connector 16 or the liquid inlet connector 17. A circular through hole penetrating the mounting rod 21 is opened perpendicularly to the mounting rod 21 in the middle of the mounting rod 21, and the sealing cone 22 can be inserted into the circular through hole on the mounting rod 21.

[0055] The sealing cone 22 is a frustum of a cone made of rubber. The diameter of the larger end of the sealing cone 22 is slightly larger than the inner diameter of the negative pressure connector 16 and the liquid inlet connector 17, and the diameter of the smaller end of the sealing cone 22 is smaller than the outer diameter of the negative pressure connector 16 and the liquid inlet connector 17. A cylindrical connecting rod 23 is fixed on the smaller end face of the sealing cone 22. The connecting rod 23 can be inserted into the circular through hole on the mounting rod 21, and the connecting rod 23 can move up and down along this circular through hole.

[0056] A circular spring mounting block 25 is fixed at the other end of the connecting rod 23. The diameter of the spring mounting block 25 is smaller than the inner diameter of the negative pressure connector 16 and the liquid inlet connector 17. The first spring 24 is sleeved on the connecting rod 23. One end of the first spring 24 abuts against the spring mounting block 25, and the other end abuts against the mounting rod 21. When the sealing cone 22 is pulled, the connecting rod 23 moves along the circular through hole on the mounting rod 21. At this time, the first spring 24 is compressed by force. After the sealing cone 22 is released, the sealing cone 22 resets under the action of the first spring 24.

[0057] The elastic sealing assembly 2 is provided in both the negative pressure connector 16 and the liquid inlet connector 17. The elastic sealing assembly 2 provided in the negative pressure connector 16 is located at the upper end of the negative pressure connector 16. The sealing cone 22 in the elastic sealing assembly 2 here is stuck on the outer end face of the negative pressure connector 16.

[0058] The elastic sealing assembly 2 provided in the liquid inlet connector 17 is located at the lower end of the liquid inlet connector 17. The sealing cone 22 in the elastic sealing assembly 2 here is stuck on the inner end face of the liquid inlet connector 17.

[0059] Regarding the sealing of the negative pressure joint 16 and the liquid inlet joint 17 by the elastic sealing assembly 2, when not in use, the sealing cone 22 is stuck at the port of the negative pressure joint 16 or the liquid inlet joint 17 under the action of the first spring 24. When negative pressure suction is applied, the sealing cone 22 in the elastic sealing assembly 2 at the negative pressure joint 16 is lifted upward under the action of the negative pressure. At this time, the first spring 24 is compressed. When continuous negative pressure is applied, the sealing cone 22 in the elastic sealing assembly 2 at the inner end of the liquid inlet joint 17 moves downward under the action of the negative pressure, opening the liquid inlet joint 17. At this time, the drainage liquid in the double-layer tube can flow into the bottle body 11 through the liquid inlet joint 17. After use, the negative pressure is closed. At this time, under the action of the first spring 24, the sealing cones 22 in the negative pressure joint 16 and the liquid inlet joint 17 are reset to re-seal the negative pressure joint 16 and the liquid inlet joint 17.

[0060] Definition: In this embodiment, with the bottle body 11 as the reference, the direction from the bottle body 11 to the bottle cap is upward, the direction from the bottle cap to the bottle body 11 is downward, the direction from the outside to the bottle body 11 is the inside, and the direction from the bottle body 11 to the outside is the outside.

[0061] As Figure 1 、 6 As shown in Figures 7, 8, 9, 10 and 12, the anaerobic culture bottle 3 includes a bottle body 11 and an anaerobic bottle cap 31, and the anaerobic bottle cap 31 is detachably screwed to the bottle body 11.

[0062] The bottle body 11 of the aerobic culture bottle 1 is exactly the same as the bottle body 11 of the anaerobic culture bottle 3, and the anaerobic bottle cap 31 is generally the same as the aerobic bottle cap 12, both including a negative pressure joint 16 and a liquid inlet joint 17. The difference between the anaerobic bottle cap 31 and the aerobic bottle cap 12 is that a functional hole 32 for assisting in installing the injection assembly 5 is provided on the anaerobic bottle cap 31, and at the same time, an air removal assembly 4 for removing air from the drainage liquid is also provided on the anaerobic bottle cap 31.

[0063] The main function of the air removal assembly 4 is to discharge the air in the drainage liquid attracted into the injection tube 501 of the injection assembly 5, and finally fill the injection tube 501 with air-free drainage liquid.

[0064] The main function of the injection assembly 5 is to inject the air-free drainage liquid into the anaerobic culture bottle 3 to facilitate the anaerobic culture of the drainage liquid.

[0065] The air removal assembly 4 includes an overflow chamber 41, a functional chamber 42 and an exhaust pipe 43. The functional chamber 42 is connected to the overflow chamber 41, and one end of the exhaust pipe 43 is connected to the functional chamber 42 and the other end is connected to the negative pressure joint 16 on the anaerobic bottle cap 31.

[0066] The overflow chamber 41 is a circular tube made of plastic. The upper and lower ends of the overflow chamber 41 are closed. The main function of the overflow chamber 41 is to store the drainage liquid overflowing from the injection tube 501.

[0067] To facilitate the connection with the negative pressure connector 16 and thus achieve negative pressure suction, an arc-shaped chamber protrudes from the upper surface of the function chamber 42. This chamber is named the function chamber 42. The lower end of the function chamber 42 communicates with the overflow chamber 41. A circular connecting pipe is provided on the upper end surface of the function chamber 42, and this connecting pipe is named the exhaust pipe 43. The exhaust pipe 43 covers the lower end of the negative pressure connector 16 on the anaerobic bottle cap 31, and a connection seal is made at the covering position. In this way, when the negative pressure pipe is connected to the negative pressure connector 16, the overflow chamber 41 will also be in a negative pressure state.

[0068] To facilitate the installation of the injection assembly 5, a circular through-hole is opened on the upper end surface of the overflow chamber 41. The injection tube 501 in the injection assembly 5 is inserted into this circular through-hole, and a seal is made at the connection between the injection tube 501 and the overflow chamber 41.

[0069] The injection assembly 5 includes an injection tube 501, an injection push rod 502, and an exhaust port sealing rod 503. One end of the injection tube 501 is fixed on the anaerobic bottle cap 31 and covers the function hole 32, and the other end is inserted into the overflow chamber 41. To ensure tightness and prevent liquid leakage and air leakage at the contact points, seals are made at all the connection points of the injection tube 501. The exhaust port sealing rod 503 is arranged in parallel with the injection push rod 502. The exhaust port sealing rod 503 passes through the push plate on the injection push rod 502 and is distributed parallel to the injection push rod 502. The injection push rod 502 and the exhaust port sealing rod 503 pass through the anaerobic bottle cap 31 and are inserted into the injection tube 501.

[0070] The injection tube 501 is a cylindrical tube, similar in shape to a syringe. The upper end of the injection tube 501 is open, and the injection push rod 502 and the exhaust port sealing rod 503 are inserted into the injection tube 501 through this opening. A injection port 505 protrudes from the lower end of the injection tube 501. The injection port 505 passes through the bottom surface of the overflow chamber 41 and communicates with the bottle body 11 of the anaerobic culture bottle 3. Through the injection port 505, air-free drainage fluid can be injected into the bottle body 11 of the anaerobic culture bottle 3.

[0071] The injection push rod 502 is similar to the push rod of a common syringe, including an injection piston 507 and a push plate. The injection piston 507 passes through the function hole 32 and is inserted into the injection tube 501. Through the injection piston 507, the drainage fluid in the injection tube 501 can be injected into the bottle body 11 of the anaerobic culture bottle 3.

[0072] To form a negative pressure suction path, a circular through-hole penetrating the surface of the injection piston 507 is provided on the surface of the injection piston 507, and this through-hole is named the second exhaust port 508. At the same time, on the injection tube 501 near the upper end of the injection tube 501 and on the side wall of the function chamber 42, a circular hole penetrating a single side wall of the injection tube 501 is provided, and this circular hole is named the first exhaust port 506. At the same time, a circular through-hole is also provided at the corresponding position of the function chamber 42. Through this circular through-hole, the first exhaust port 506 and the second exhaust port 508, the liquid overflow chamber 41 can be communicated with the injection tube 501.

[0073] On the side wall of the injection tube 501 near the upper end, a circular hole penetrating a single side wall of the injection tube 501 is provided, and this circular hole is named the liquid inlet 504. The height of the liquid inlet 504 is slightly lower than the height of the first exhaust port 506. An "L"-shaped pipe is connected to the liquid inlet 504, and this pipe is named the liquid inlet pipe 512. One end of the liquid inlet pipe 512 is connected to the liquid inlet 504, and the other end covers the lower end face of the anaerobic bottle cap 31, covering the lower port of the liquid inlet joint 17. And to ensure that the sealing cone 22 in the elastic sealing assembly 2 in the liquid inlet joint 17 can be opened smoothly, the inner diameter of the liquid inlet pipe 512 is larger than the outer diameter of the sealing cone 22.

[0074] A path is formed through the liquid inlet pipe 512, the liquid inlet 504, the second exhaust port 508, the first exhaust port 506 and the exhaust pipe 43. When negative pressure suction is performed, the drainage liquid in the double-layer tube can be sucked into the injection tube 501 through the liquid inlet joint 17 and the liquid inlet pipe 512, and at the same time, the air sucked in together with the drainage liquid can be discharged through the first exhaust port 506 and the second exhaust port 508.

[0075] When the injection tube 501 is filled with drainage liquid and contains no air, to prevent the drainage liquid from entering the liquid overflow chamber 41 through the first exhaust port 506 and the second exhaust port 508 during the injection process, the second exhaust port 508 can be sealed by using the exhaust port sealing rod 503.

[0076] The exhaust port sealing rod 503 includes a sealing head 509. The sealing head 509 is made of rubber material, and the size of the sealing head 509 is slightly larger than the size of the second exhaust port 508. The sealing head 509 is fixed at one end of the exhaust port sealing rod 503. By pressing the exhaust port sealing rod 503, the sealing head 509 can be inserted into the second exhaust port 508 to seal the second exhaust port 508.

[0077] In order to make the closing of the second exhaust port 508 controllable, an annular limit ring 511 is fixed on the rod body of the exhaust port sealing rod 503. At the same time, a spring is sleeved outside the exhaust port sealing rod 503, and this spring is named the second spring 510. One end of the second spring 510 abuts against the anaerobic bottle cap 31, and the other end abuts against the limit ring 511. When the exhaust port sealing rod 503 does not close the second exhaust port 508, the exhaust port sealing rod 503 is suspended under the action of the second spring 510. The opening of the second exhaust port 508 is the initial state. In the initial state, the injection piston 507 is located between the liquid inlet 504 and the first exhaust port 506.

[0078] During negative pressure suction, in order to prevent the gas in the bottle body 11 of the anaerobic culture bottle 3 from being sucked away, a sealing piece 513 is hinged on the overflow chamber 41 through a hinge platform. One end of the sealing piece 513 is hinged on the hinge platform, and the other end covers the injection port 505 to close the injection port 505. In order to make the sealing piece 513 also close the injection port 505 under normal conditions, a torsion spring is sleeved on the hinge rod of the sealing piece 513. A force is applied to the sealing piece 513 through the torsion spring, so that the sealing piece 513 can close the injection port 505 under normal conditions. When injecting through the injection tube 501, the liquid in the injection tube 501 squeezes the sealing piece 513, causing the sealing piece 513 to open along the hinge axis. When the injection stops, the sealing piece 513 closes the injection port 505 under the action of the torsion spring.

[0079] As Figure 6 、 11 As shown in 12, in order to make the injection of the injection push rod 502 controllable, a push rod limit assembly 7 is provided on the anaerobic bottle cap 31. The push rod limit assembly 7 includes a limit rod 71 and a limit card 73.

[0080] The limit rod 71 is a rectangular rod made of plastic. One end of the limit rod 71 is fixed on the upper surface of the anaerobic bottle cap 31, and the other end passes through the push plate on the injection push rod 502. The injection push rod 502 can move up and down along the limit rod 71.

[0081] On one side surface of the limit rod 71, a plurality of rectangular grooves are recessed perpendicular to the limit rod 71. These rectangular grooves are named limit grooves 72, and a plurality of limit grooves 72 are arranged at intervals on the limit rod 71.

[0082] The limit card 73 is a card with a "Z"-shaped cross-section. The limit card 73 is hinged on the push plate in the injection push rod 502 through a hinge platform. One end of the limit card 73 can be stuck in the limit groove 72 to limit the injection push rod 502. In order to make the limit of the injection push rod 502 by the limit card 73 controllable, a torsion spring is sleeved on the hinge shaft of the limit card 73. Under the action of the torsion spring, one end of the limit card 73 is always stuck in the limit groove 72. Only by manually pressing the other end of the limit card 73 can the clamping connection between the limit card 73 and the limit groove 72 be released, thereby realizing the controllable control of the injection of the injection push rod 502.

[0083] Regarding the process of sucking the drainage fluid into the anaerobic culture bottle 3 by negative pressure: During use, first unscrew the seal 19 on the anaerobic bottle cap 31, then screw the negative pressure tube onto the negative pressure connector 16, connect the double tube to the liquid inlet connector 17, and then turn on the negative pressure gauge on the negative pressure tube. At this time, under the action of negative pressure, the drainage fluid in the double tube flows into the injection tube 501 through the liquid inlet connector 17 and the liquid inlet pipe 512. Then continue the negative pressure suction until the injection tube 501 is filled with drainage fluid. The overflowing drainage fluid flows into the overflow chamber 41 through the first exhaust port 506 and the second exhaust port 508. At this time, the negative pressure suction can be stopped. Then first press the exhaust port sealing rod 503 to use the sealing head 509 on the exhaust port sealing rod 503 to seal the second exhaust port 508. Then press the limit card 73 on the push rod limit assembly 7 to release the restriction on the position of the injection push rod 502. Then push the injection push rod 502. The drainage fluid in the injection tube 501 squeezes the sealing piece 513 at the injection port 505, and the sealing piece 513 opens, and the drainage fluid in the injection tube 501 can be injected into the bottle body 11 of the anaerobic culture bottle 3. After injecting an appropriate amount, stop pushing the injection push rod 502 and release the limit card 73. At this time, the limit card 73 is stuck in the limit groove 72 in the limit rod 71 to limit the position of the injection push rod 502;

[0084] When the drainage fluid in the double tube is not enough to fill the injection tube 501, do not seal the second exhaust port 508 before pushing the injection push rod 502. Open the seal 19 on the negative pressure connector 16. At this time, the gas in the injection tube 501 will first be discharged from the negative pressure connector 16 (here, the elastic force of the torsion spring in the sealing piece 513 needs to be greater than the elastic force of the first spring 24 in the negative pressure connector 16) until part of the drainage fluid flows into the overflow chamber 41, and then seal the second exhaust port 508 for injection, ensuring that the drainage fluid injected into the bottle body 11 of the anaerobic culture bottle 3 does not contain air, ensuring the accuracy of anaerobic culture.

[0085] In addition, when the injection push rod 502 injects into the bottle body 11, in order to facilitate the smooth entry of the drainage fluid in the injection tube 501 into the bottle body 11, a one-way valve can be installed on the bottle body 11 of the anaerobic culture bottle 3. During injection, a part of the gas in the bottle body 11 (this gas is pre-filled inert gas, which is the prior art) is discharged through the one-way valve. At this time, the drainage fluid in the injection tube 501 can be smoothly injected into the bottle body 11.

[0086] To facilitate the observation of the amount of the drainage fluid, both the injection tube 501 and the overflow chamber 41 are preferably made of transparent materials.

[0087] As Figure 13 shown, the culture bottle placement box 6 is a box with a rectangular cross-section. The upper end of the culture bottle placement box 6 is open, and the aerobic culture bottle 1 and the anaerobic culture bottle 3 can be placed in the culture bottle placement box 6.

[0088] In the middle of the culture bottle placement box 6, a rectangular partition 61 protrudes vertically from the bottom surface of the culture bottle placement box 6. The culture bottle placement box 6 is divided into two culture bottle placement cavities 63 of the same size by the partition 61. The aerobic culture bottle 1 and the anaerobic culture bottle 3 are respectively placed in the two culture bottle placement cavities 63.

[0089] To facilitate the observation of the aerobic culture bottle 1 and the anaerobic culture bottle 3 placed in the culture bottle placement cavity 63, a rectangular observation window 62 is respectively opened on the two side surfaces of the culture bottle placement box 6. Through the observation window 62, the aerobic culture bottle 1 and the anaerobic culture bottle 3 placed in the culture bottle placement cavity 63 can be observed.

[0090] The usage process of this embodiment:

[0091] Drainage fluid collection of the aerobic culture bottle 1: Unscrew the caps 19 on the negative pressure connector 16 and the liquid inlet connector 17 on the aerobic culture bottle cap 12, screw the negative pressure tube onto the negative pressure connector 16, connect the double tube connected to the patient to the liquid inlet connector 17, and open the negative pressure gauge connected to the negative pressure tube. Under the suction of the negative pressure gauge, the elastic sealing assembly 2 in the negative pressure connector 16 and the liquid inlet connector 17 opens, and the drainage fluid flows into the aerobic culture bottle 1 through the liquid inlet connector 17;

[0092] Judge the amount of the drainage fluid flowing into the aerobic culture bottle 1 according to the scale 13 on the bottle body 11. When appropriate, close the negative pressure gauge. At this time, the elastic sealing assembly 2 in the negative pressure connector 16 and the liquid inlet connector 17 closes. Remove the double tube and the negative pressure tube from the negative pressure connector 16 and the liquid inlet connector 17, and use the cap 19 to seal the negative pressure connector 16 and the liquid inlet connector 17 to complete the collection of the drainage fluid of the aerobic culture bottle 1.

[0093] Anaerobic culture bottle 3 drainage fluid collection: Unscrew the caps 19 on the negative pressure connector 16 and the liquid inlet connector 17 on the anaerobic bottle cap 31. Screw the negative pressure tube onto the negative pressure connector 16, connect the double cannula connected to the patient to the liquid inlet connector 17, and open the negative pressure gauge connected to the negative pressure tube. Under the suction of the negative pressure gauge, the elastic sealing assembly 2 inside the negative pressure connector 16 and the liquid inlet connector 17 opens. The negative pressure gauge sucks the drainage fluid to flow into the syringe tube 501 through the liquid inlet tube 512. After the drainage fluid enters the syringe tube 501, it accumulates below the syringe tube 501, and the air in the drainage fluid is above the syringe tube 501. As the drainage fluid gradually enters the syringe tube 501, the air above the syringe tube 501 is discharged through the second exhaust port 508 and the first exhaust port 506 until the syringe tube 501 is filled with the drainage fluid. The overflowing drainage fluid is discharged into the overflow chamber 41 through the second exhaust port 508 and the first exhaust port 506;

[0094] When the drainage fluid in the double cannula is not enough to fill the syringe tube 501, before pushing the syringe plunger 502, do not seal the second exhaust port 508 first. Open the cap 19 on the negative pressure connector 16. At this time, the gas in the syringe tube 501 will be discharged from the negative pressure connector 16 first until part of the drainage fluid flows into the overflow chamber 41, and then seal the second exhaust port 508 for injection.

[0095] Press the limit card 73 to unlock the syringe plunger 502, and then push the syringe plunger 502 to inject the drainage fluid in the syringe tube 501 into the bottle body 11 of the anaerobic culture bottle 3. Judge the amount of injected drainage fluid according to the scale 13 on the bottle body 11. Stop injecting after the amount is appropriate. Close the negative pressure tube, remove the double cannula and the negative pressure tube from the negative pressure connector 16 and the liquid inlet connector 17, and use the cap 19 to seal the negative pressure connector 16 and the liquid inlet connector 17 to complete the collection of the drainage fluid of the anaerobic culture bottle 3.

[0096] The above embodiments are only to illustrate the technical idea of the present invention, and the protection scope of the present invention cannot be limited thereby. Any modification made on the basis of the technical solution according to the technical idea proposed by the present invention falls within the protection scope of the present invention.

Claims

1. A double-cannula continuous flushing negative pressure suction liquid culture medium set, characterized in that: It includes an aerobic culture bottle (1), an anaerobic culture bottle (3) and a culture bottle placement box (6). The aerobic culture bottle (1) storing aerobic culture medium is detachably arranged in the culture bottle placement box (6), and the anaerobic culture bottle (3) storing anaerobic culture medium is detachably arranged in the culture bottle placement box (6); The aerobic culture bottle (1) includes a bottle body (11) and an aerobic bottle cap (12). The aerobic bottle cap (12) is detachably connected to the bottle body (11). The aerobic bottle cap (12) includes a negative pressure connector (16) and a liquid inlet connector (17). The negative pressure connector (16) and the liquid inlet connector (17) are controllably sealed by a sealing cover (19). The negative pressure connector (16) is connected to an external negative pressure tube, and the liquid inlet connector (17) is connected to an external double tube. The liquid in the double tube flows into the bottle body (11) through the liquid inlet connector (17); The anaerobic culture bottle (3) includes a bottle body (11) and an anaerobic bottle cap (31). The anaerobic bottle cap (31) is detachably connected to the bottle body (11). The anaerobic bottle cap (31) includes an air removal component (4) and an injection component (5). The injection component (5) is connected to the liquid inlet connector (17) on the anaerobic bottle cap (31). The air removal component (4) is connected to the negative pressure connector (16) on the anaerobic bottle cap (31). The air removal component (4) is communicated with the injection component (5). The air removal component (4) discharges the air sucked into the injection component (5), and the injection component (5) injects the air-free suction liquid into the bottle body (11) storing anaerobic bacteria culture medium.

2. The double cannula continuous flushing negative pressure suction liquid culture medium set according to claim 1, characterized in that: An elastic sealing component (2) is arranged on the aerobic bottle cap (12) and the anaerobic bottle cap (31). The elastic sealing component (2) includes a mounting rod (21), a sealing cone (22) and a first spring (24). The sealing cone (22) is inserted on the mounting rod (21). The mounting rod (21) is arranged in the negative pressure connector (16) and the liquid inlet connector (17). The first spring (24) is sleeved on the sealing cone (22). The sealing cone (22) moves controllably along the mounting rod (21) to control the opening and closing of the negative pressure connector (16) and the liquid inlet connector (17).

3. The double-cannula continuous irrigation negative pressure aspiration liquid culture medium set according to claim 2, wherein: The elastic sealing component (2) is arranged on the negative pressure connector (16) and the liquid inlet connector (17) of both the aerobic bottle cap (12) and the anaerobic bottle cap (31). The sealing cone (22) of the elastic sealing component (2) arranged in the negative pressure connector (16) closes the upper end of the negative pressure connector (16), and the sealing cone (22) of the elastic sealing component (2) in the liquid inlet connector (17) closes the lower end of the liquid inlet connector (17).

4. The double-cannula continuous flushing negative pressure aspiration liquid culture medium set according to claim 1, wherein: The injection component (5) includes an injection tube (501) and an injection push rod (502). The injection push rod (502) is inserted in the injection tube (501). The injection tube (501) is arranged on the anaerobic bottle cap (31), and the injection push rod (502) passes through the anaerobic bottle cap (31) and exposes outside the anaerobic bottle cap (31).

5. The double-cannula continuous flushing negative pressure aspiration liquid culture medium set according to claim 4, characterized in that: A liquid inlet (504) is opened on the side wall of the injection tube (501), and a liquid inlet pipe (512) is arranged on the liquid inlet (504). One end of the liquid inlet pipe (512) is connected to the liquid inlet (504), and the other end is connected to the liquid inlet connector (17) on the anaerobic bottle cap (31).

6. The double-cannula continuous flushing negative pressure aspiration liquid culture medium set according to claim 4, wherein: A first exhaust port (506) is provided on the side wall of the syringe tube (501). The first exhaust port (506) is communicated with the air scavenging assembly (4). A second exhaust port (508) is provided on the injection piston (507) of the injection push rod (502). The second exhaust port (508) is communicated with the first exhaust port (506), and the second exhaust port (508) can be controllably opened and closed by an exhaust port sealing rod (503) mounted on the injection push rod (502).

7. The double cannula continuous flushing negative pressure suction liquid culture medium set according to claim 1, characterized in that: The air scavenging assembly (4) includes an overflow chamber (41), a functional chamber (42) and an exhaust pipe (43). The overflow chamber (41) is sleeved outside the syringe tube (501). The functional chamber (42) is connected to the overflow chamber (41). An exhaust port communicated with the first exhaust port (506) is provided on the functional chamber (42). An exhaust pipe (43) is provided on the functional chamber (42), and the exhaust pipe (43) is connected to a negative pressure joint (16) on the anaerobic bottle cap (31).

8. The double-cannula continuous flushing negative pressure aspiration liquid culture medium set according to claim 1, wherein: The bottle body (11) is made of a transparent material, and a scale (13) for observing the quantity of the liquid in the bottle body (11) is provided on the side wall of the bottle body (11).

9. The double-cannula continuous flushing negative pressure suction liquid culture medium set according to claim 1, wherein: The culture bottle placement box (6) includes a partition board (61) and an observation window (62). The partition board (61) is arranged inside the culture bottle placement box (6) and divides the culture bottle placement box (6) into two culture bottle placement cavities (63). The observation windows (62) are opened on both sides of the culture bottle placement box (6), and the liquid volume in the bottle body (11) is observed through the observation windows (62).

Images