Extraction equipment capable of easily preserving SOD (superoxide dismutase) enzyme activity

Abstract

The utility model relates to the technical field of SOD (superoxide dismutase) enzyme preservation and extraction, in particular to extraction equipment easy for SOD enzyme activity preservation, which comprises a main body frame, a cooling-water machine is arranged on the inner side surface of the main body frame, and a fixing ring is arranged in the main body frame; an ultrasonic device is arranged at the top of the operation chamber, a feeding pipe is arranged at the top of the operation chamber, and a discharging pipe is arranged at the bottom of the operation chamber; the device has the beneficial effects that a material can be poured into the operation chamber through the feeding pipe, an ultrasonic device is arranged at the top in the operation chamber, cells are broken through ultrasonic waves, the cells are broken to generate SOD enzyme, then the generated SOD enzyme can enter the discharging pipe through the ultrafiltration membrane, in the process, ice water is contained in the heat preservation tank, and the SOD enzyme can be discharged through the water suction pump. Water in the heat preservation tank and the transfer frame is circulated, and the water cooler is arranged on the surface of the transfer frame, so that it is guaranteed that the interior of the operation chamber is in a low-temperature environment all the time, and the activity of SOD enzyme is maintained while the SOD enzyme is extracted.

Description

Technical Field

[0001] The utility model relates to the field of SOD enzyme preservation and extraction, and specifically relates to an extraction device for easily preserving the activity of SOD enzyme. Background Art

[0002] SOD enzyme extraction equipment plays a crucial role in the biotech and pharmaceutical industries. The design and application of such equipment are directly related to the extraction efficiency, purity, and activity retention of SOD enzyme. Efficient extraction equipment can adopt advanced separation technologies, such as membrane filtration and centrifugal separation, to ensure extraction under mild conditions, thereby maximizing the retention of enzyme activity and structural integrity. In addition, the automation level and precise control function of the equipment are also key factors affecting the extraction effect, which can reduce human operation errors and achieve standardization and repeatability of the extraction process.

[0003] In the prior art, traditional SOD enzyme extraction equipment mainly relies on mechanical crushing or chemical treatment methods to break cell walls and release SOD enzyme, and then physical methods such as filtration, precipitation, or centrifugation are used to separate and purify the enzyme solution, thereby ensuring the effective extraction and purification of SOD enzyme.

[0004] However, when using traditional SOD enzyme extraction equipment, since SOD enzyme is relatively easy to inactivate, when the operating environment is not properly controlled, such as too high temperature will accelerate the denaturation and loss of enzyme activity, which leads to low extraction efficiency and low product purity, and further affects subsequent application research and the quality of biological products. Therefore, the utility model proposes an extraction device for easily preserving the activity of SOD enzyme to solve the above-mentioned technical problems. Content of the Utility Model

[0005] The purpose of the utility model is to provide an extraction device for easily preserving the activity of SOD enzyme to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the utility model provides the following technical solution: an extraction device for easily preserving the activity of SOD enzyme, the extraction device for easily preserving the activity of SOD enzyme includes: a main frame, a chiller is arranged on the inner side surface of the main frame, and a fixing ring is arranged inside the main frame;

[0007] An operation chamber, an ultrasonic device is arranged on the top of the operation chamber, a feeding pipe is arranged on the top of the operation chamber, and a discharging pipe is arranged on the bottom of the operation chamber.

[0008] Preferably, a moisture-proof bracket is arranged at the bottom of the main frame, a fixing block is arranged on the inner side surface of the main frame, the fixing ring is fixedly connected to the surface of the fixing block, and the operation chamber is arranged inside the fixing ring.

[0009] Preferably, a transfer frame is provided on the surface of the chiller, the surface of the transfer frame is fixedly connected to the inner side surface of the main frame, a water outlet pipe is provided at the bottom of the transfer frame, a sealing cover is provided at one end of the water outlet pipe, water absorption holes are provided on the surface of the transfer frame, a water absorption pipe is provided in the water absorption holes, a water absorption pump is provided at one end of the water absorption pipe, a threaded pipe is provided at the other end of the water absorption pipe, the threaded pipe is externally screwed with a water absorption pipe, a transmission hole is provided on the surface of the transfer frame, and a transmission pipe is provided in the transmission hole.

[0010] Preferably, holes are provided on the surface of the operation chamber, an ultrasonic device is provided in the holes, holes are provided at the top of the operation chamber, a feeding pipe is provided in the holes, a sealing cover is provided at the top of the feeding pipe, an ultrafiltration membrane is provided in the operation chamber, a discharge hole is provided at the bottom of the operation chamber, and a heat preservation tank is provided in the operation chamber.

[0011] Preferably, holes are provided on the surface of the heat preservation tank, a water absorption pipe is provided in the holes, a transmission hole is provided on the surface of the heat preservation tank, a transmission pipe is provided in the transmission hole, and a water outlet pipe is provided at the top of the heat preservation tank.

[0012] Preferably, a clamping block is provided in the discharge pipe, a spherical valve is clamped in the clamping block, the spherical valve can rotate in the clamping block, a hole is provided on the surface of the discharge pipe, a rotating rod is provided in the hole, and one end of the rotating rod is fixedly connected to the surface of the spherical valve.

[0013] Compared with the prior art, the beneficial effects of the present utility model are:

[0014] The extraction device for easily preserving the activity of SOD enzyme proposed by the present utility model, when in use, the material can be poured into the operation chamber through the feeding pipe. An ultrasonic device is provided at the top of the operation chamber, and ultrasonic waves are used to break cells. The broken cells produce SOD enzyme. Then, the produced SOD enzyme will pass through the ultrafiltration membrane and enter the discharge pipe. During this process, ice water is contained in the heat preservation tank, and through the action of the water absorption pump, the water in the heat preservation tank and the transfer frame is circulated. A chiller is provided on the surface of the transfer frame, so as to ensure that the operation chamber is always in a low-temperature environment, thereby realizing the maintenance of the activity of SOD enzyme while extracting it. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is a schematic structural diagram of the present utility model;

[0016] Figure 2 is an overall sectional view of the operation chamber of the structure of the present utility model;

[0017] Figure 3 is a schematic diagram of the internal structure of the operation chamber of the structure of the present utility model;

[0018] Figure 4 is a schematic diagram of the overall structure of the spherical valve of the structure of the present utility model.

[0019] In the figure: 1. Main body frame; 2. Chiller; 3. Fixed ring; 4. Operation room; 5. Ultrasonic device; 6. Feeding pipe; 7. Discharge pipe; 8. Fixed block; 9. Transfer frame; 10. Water outlet pipe; 11. Sealing cover;

[0020] 12. Suction pipe; 13. Suction pump; 14. Transmission pipe; 15. Ultrafiltration membrane; 16. Ball valve; 17. Insulation tank; 18. Rotating rod; 19. Clamping block. Specific embodiments

[0021] In order to clearly and completely describe the purpose, technical solution of the present utility model and make the advantages more clear, the following further details the embodiments of the present utility model with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are part of the embodiments of the present utility model, rather than all embodiments, and are only used to explain the embodiments of the present utility model, not to limit the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present utility model.

[0022] Embodiment 1: Please refer to Figures 1 - 4 , the present utility model provides a technical solution: An extraction device for easy preservation of SOD enzyme activity, the extraction device for easy preservation of SOD enzyme activity includes: a main body frame 1, a chiller 2 is arranged on the inner side surface of the main body frame 1, and a fixed ring 3 is arranged inside the main body frame 1;

[0023] An operation room 4, an ultrasonic device 5 is arranged on the top of the operation room 4, a feeding pipe 6 is arranged on the top of the operation room 4, and a discharge pipe 7 is arranged at the bottom of the operation room 4;

[0024] During use, materials can be poured into the operation room 4 through the feeding pipe 6. An ultrasonic device 5 is arranged on the top of the operation room 4, and ultrasonic waves are used to break cells. The broken cells produce SOD enzyme. Then, the produced SOD enzyme will pass through the ultrafiltration membrane 15 and enter the discharge pipe 7. During this process, ice water is contained in the insulation tank 17. Through the action of the suction pump 13, the water in the insulation tank 17 and the transfer frame 9 is circulated. And a chiller 2 is arranged on the surface of the transfer frame 9, so as to ensure that the operation room 4 is always in a low-temperature environment, thereby realizing the maintenance of the activity of SOD enzyme while extracting it.

[0025] Embodiment 2: On the basis of Embodiment 1, in order to enable the extraction device to better extract SOD enzyme, an operation room 4 is provided. Holes are opened on the surface of the operation room 4, and an ultrasonic device 5 is arranged in the holes. Holes are opened on the top of the operation room 4, and a feeding pipe 6 is arranged in the holes. A sealing cover 11 is arranged on the top of the feeding pipe 6. An ultrafiltration membrane 15 is arranged inside the operation room 4. A discharge hole is opened at the bottom of the operation room 4, and an insulation tank 17 is arranged inside the operation room 4;

[0026] A moisture-proof bracket is provided at the bottom of the main frame 1, and a fixing block 8 is provided on the inner side of the main frame 1. A fixing ring 3 is fixedly connected to the surface of the fixing block 8, and an operation chamber 4 is provided inside the fixing ring 3;

[0027] A clamping block 19 is provided inside the discharge pipe 7, and a spherical valve 16 is clamped into the clamping block 19. The spherical valve 16 can rotate inside the clamping block 19. A hole is provided on the surface of the discharge pipe 7, and a rotating rod 18 is provided inside the hole. One end of the rotating rod 18 is fixedly connected to the surface of the spherical valve 16

[0028] To ensure that the extraction device can successfully extract SOD enzyme, an ultrasonic device 5 is installed at the top inside the operation chamber 4. This device uses ultrasonic energy to achieve efficient cell disruption, thereby releasing SOD enzyme. Subsequently, the released SOD enzyme molecules pass through the microporous structure of the ultrafiltration membrane 15 under the designed process and are effectively collected inside the discharge pipe 7. If it is necessary to take out the SOD enzyme, the spherical valve 16 can be rotated by rotating the rotating rod 18, thereby taking out the SOD enzyme.

[0029] Example 3: On the basis of Example 2, a chiller 2 is provided to ensure the activity of SOD enzyme during the extraction process. A transfer frame 9 is provided on the surface of the chiller 2. The surface of the transfer frame 9 is fixedly connected to the inner side of the main frame 1. A water outlet pipe 10 is provided at the bottom of the transfer frame 9. One end of the water outlet pipe 10 is provided with a sealing cover 11. A water absorption hole is provided on the surface of the transfer frame 9, and a water absorption pipe 12 is provided inside the water absorption hole. One end of the water absorption pipe 12 is provided with a water suction pump 13, and the other end of the water absorption pipe 12 is provided with a threaded pipe. The threaded pipe is externally screwed with the water absorption pipe 12. A transfer hole is provided on the surface of the transfer frame 9, and a transfer pipe 14 is provided inside the transfer hole;

[0030] A hole is provided on the surface of the insulation tank 17, and a water absorption pipe 12 is provided inside the hole. A transfer hole is provided on the surface of the insulation tank 17, and a transfer pipe 14 is provided inside the transfer hole. A water outlet pipe 10 is provided at the top of the insulation tank 17;

[0031] To ensure the activity of the enzyme during the extraction of SOD enzyme, the ice water filled in the insulation tank 17 is driven by the water suction pump 13 to form a closed circulation with the water flow inside the transfer frame 9. A chiller 2 is assembled outside the transfer frame 9 to further enhance the cooling effect, thereby creating an ideal low-temperature condition conducive to the stable extraction of SOD enzyme, thus continuously maintaining the low-temperature environment inside the operation chamber 4 and ensuring that the activity of SOD enzyme is not damaged.

[0032] In actual use, the material can be poured into the operation chamber 4 through the feeding pipe 6. An ultrasonic device 5 is arranged at the top of the operation chamber 4, and ultrasonic waves are used to break cells. The broken cells produce SOD enzyme, and then the produced SOD enzyme will pass through the ultrafiltration membrane 15 and enter the discharge pipe 7. During this process, ice water is contained in the heat preservation tank 17. Through the action of the water suction pump 13, the water in the heat preservation tank 17 and the transfer frame 9 is circulated. A chiller 2 is arranged on the surface of the transfer frame 9, so as to ensure that the operation chamber 4 is always in a low-temperature environment, thus realizing the maintenance of the activity of SOD enzyme while extracting it.

[0033] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An extraction device for facilitating the preservation of SOD enzyme activity, characterized in that: The extraction device for facilitating the preservation of SOD enzyme activity includes: a main body frame (1), a chiller (2) is arranged on the inner side surface of the main body frame (1), and a fixing ring (3) is arranged inside the main body frame (1); An operation chamber (4), an ultrasonic device (5) is arranged on the top of the operation chamber (4), a feeding pipe (6) is arranged on the top of the operation chamber (4), and a discharging pipe (7) is arranged at the bottom of the operation chamber (4).

2. An extraction device for easily preserving the activity of SOD enzyme according to claim 1, characterized in that: A moisture-proof support is arranged at the bottom of the main body frame (1), a fixing block (8) is arranged on the inner side surface of the main body frame (1), a fixing ring (3) is fixedly connected to the surface of the fixing block (8), and an operation chamber (4) is arranged inside the fixing ring (3).

3. An extraction device for easily preserving the activity of SOD enzyme according to claim 1, characterized in that: A transfer frame (9) is arranged on the surface of the chiller (2), the surface of the transfer frame (9) is fixedly connected to the inner side surface of the main body frame (1), a water outlet pipe (10) is arranged at the bottom of the transfer frame (9), a sealing cover (11) is arranged at one end of the water outlet pipe (10), a water absorption hole is formed on the surface of the transfer frame (9), a water absorption pipe (12) is arranged inside the water absorption hole, a water absorption pump (13) is arranged at one end of the water absorption pipe (12), a threaded pipe is arranged at the other end of the water absorption pipe (12), the water absorption pipe (12) is externally screwed to the threaded pipe, and a transfer pipe (14) is arranged inside a transfer hole formed on the surface of the transfer frame (9).

4. An extraction device for easily preserving the activity of SOD enzyme according to claim 1, characterized in that: Holes are formed on the surface of the operation chamber (4), and an ultrasonic device (5) is arranged inside the holes. A hole is formed on the top of the operation chamber (4), and a feeding pipe (6) is arranged inside the hole. A sealing cover (11) is arranged at the top of the feeding pipe (6). An ultrafiltration membrane (15) is arranged inside the operation chamber (4). A discharging hole is formed at the bottom of the operation chamber (4), and a heat preservation tank (17) is arranged inside the operation chamber (4).

5. An extraction device for easily preserving the activity of SOD enzyme according to claim 4, characterized in that: Holes are formed on the surface of the heat preservation tank (17), and a water absorption pipe (12) is arranged inside the holes. A transfer hole is formed on the surface of the heat preservation tank (17), and a transfer pipe (14) is arranged inside the transfer hole. A water outlet pipe (10) is arranged at the top of the heat preservation tank (17).

6. An extraction device for easily preserving the activity of SOD enzyme according to claim 1, characterized in that: A clamping block (19) is arranged inside the discharging pipe (7), a spherical valve (16) is clamped inside the clamping block (19), the spherical valve (16) can rotate inside the clamping block (19), a hole is formed on the surface of the discharging pipe (7), and a rotating rod (18) is arranged inside the hole. One end of the rotating rod (18) is fixedly connected to the surface of the spherical valve (16).

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