Detection kit for induced pluripotent stem cell marker

Abstract

The utility model discloses an induced pluripotent stem cell marker detection kit which comprises a kit body, a kit turning cover is connected to the kit body, a placing frame is arranged in the kit body, reagent bottles are inserted into the placing frame, a lock hole is formed in the surface of the kit turning cover, a lock column is arranged in the lock hole, and a reagent bottle is inserted into the lock column. The lock column is fixed on the surface of the box body; through the designed assembly cylinder, the extrusion column and the spring, the assembly cylinder provided with the extrusion column and the spring is installed on the box turning cover, reagent bottles are placed in the placing frame in the box body, the box turning cover is covered, the bent movable end of the box turning cover is adjusted, the lock hole of the box turning cover is clamped on the lock column, and the box turning cover is fixed. Meanwhile, the extrusion columns are arranged at the tops of the corresponding reagent bottles, the springs apply elastic force to the extrusion columns to extrude the extrusion columns, the extrusion columns are pushed to be tightly pressed on the reagent bottles, the reagent bottles in the box are fixed, and the situation that the reagent bottles shake at will during transportation in the box, consequently, the bottle bodies are prone to being collided and damaged, and reagent liquid leaks out is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of detection kits, and particularly relates to a detection kit for induced pluripotent stem cell markers. Background Technique

[0002] The detection kit for induced pluripotent stem cell immune cell markers can perform better image-based analysis on two key markers of human pluripotent stem cells: the markers are OCT4 and SSEA4 respectively. This high-performance immunohistochemistry technology kit includes a complete set of primary antibodies and secondary antibodies, a nuclear DNA stain, and a pre-prepared buffer solution for optimizing the staining experiment.

[0003] In the existing detection kit for induced pluripotent stem cell markers, a placement rack is arranged inside, and reagent bottle structures containing a complete set of primary antibodies and secondary antibodies, a nuclear DNA stain, and a pre-prepared buffer solution are respectively inserted on the placement rack. By covering a box flip cover structure with one end that can be flipped to the port of the box body, and then flipping the movable end of the box flip cover, the lock post on the box body is inserted into the lock hole on the box flip cover for fixation. However, there are the following defects: (1) During the transportation of the detection kit for induced pluripotent stem cell markers, when the transportation tool passes through a bumpy section, the box body shakes, which easily causes the reagent bottles inside to shake up and down and fall off the placement rack, resulting in the problem that the reagent bottles collide with each other and are damaged, causing the liquid to leak out. For this reason, we propose a detection kit for induced pluripotent stem cell markers. Content of the Utility Model

[0004] The purpose of the utility model is to provide a detection kit for induced pluripotent stem cell markers, so as to solve the problem that the reagent bottles in the detection kit for induced pluripotent stem cell markers are prone to swing and collide and be damaged during transportation, resulting in liquid leakage as mentioned in the above background technique.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A detection kit for induced pluripotent stem cell markers, including a box body, a box flip cover is connected to the box body, a placement rack is arranged inside the box body, reagent bottles are inserted on the placement rack, a lock hole is opened on the surface of the box flip cover, a lock post is arranged inside the lock hole, the lock post is fixed on the surface of the box body, an assembly cylinder is fixedly embedded on the surface of the box flip cover, a pressing column is connected inside the assembly cylinder in a sliding manner, the bottom end of the pressing column is located on the top of the reagent bottle, and a spring is arranged at the top end of the pressing column, and the spring is located inside the assembly cylinder.

[0006] Preferably, a blocking hole is opened on the side wall of the pressing column, a blocking block is arranged inside the blocking hole, and the blocking block is fixed on the inner wall of the assembly cylinder.

[0007] Preferably, the cross-sections of the extrusion column and the assembly cylinder are both circular structures, and the central axes of the extrusion column and the assembly cylinder coincide with each other.

[0008] Preferably, a rubber block is fixed on the bottom surface of the extrusion column, and the cross-section of the rubber block is a circular structure.

[0009] Preferably, a threaded hole is provided on the top surface of the assembly cylinder, a stud is threadedly connected inside the threaded hole, one end of the stud is connected with a movable plate, and the movable plate is at one end of a spring.

[0010] Preferably, the cross-sections of the movable plate and the stud are both circular structures, and the central axes of the movable plate and the stud coincide with each other.

[0011] Preferably, a screwing groove is provided on the top surface of the stud, and the cross-section of the screwing groove is a hexagonal structure.

[0012] Compared with the prior art, the beneficial effects of the present utility model are:

[0013] (1) Through the designed assembly cylinder, extrusion column and spring, the assembly cylinder equipped with the extrusion column and the spring is installed on the box flip cover. The reagent bottle is placed in the placement rack inside the box body, the box flip cover is covered, and the bent movable end of the box flip cover is adjusted so that its lock hole is stuck on the lock post to fix the box flip cover. At the same time, the extrusion column is placed on the top of the corresponding reagent bottle, and the spring applies an elastic force to the extrusion column to push the extrusion column to press tightly on the reagent bottle, realizing the fixation of the reagent bottle in the box, preventing the reagent bottle from shaking randomly during transportation in the box and easily causing the bottle body to collide and be damaged, resulting in the leakage of the reagent liquid. Through the designed stop hole and stop block, the extrusion column is limited to prevent the extrusion column from detaching from the assembly cylinder. Through the designed rubber block, a soft contact buffering effect is achieved, avoiding the direct extrusion and fixation of the reagent bottle by the extrusion column and easily causing damage to the reagent bottle. Through the designed threaded hole, stud and movable plate, the stud is screwed, and the threaded connection between the stud and the threaded hole is adjusted to drive the movable plate to squeeze the spring and adjust its compression deformation degree to achieve the best elastic extrusion effect. Through the designed screwing groove, it is convenient for tools to be inserted into the groove to screw and adjust the stud. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is the front view of the present utility model;

[0015] Figure 2 is the partial structural sectional view of the box body, box flip cover, extrusion column and assembly cylinder of the present utility model;

[0016] Figure 3 is the assembled three-dimensional view of the box flip cover, assembly cylinder and extrusion column of the present utility model;

[0017] Figure 4This is the top view of the assembly of the movable plate and the stud of the present utility model;

[0018] In the figure: 1. Box body; 2. Box flip cover; 3. Lock hole; 4. Lock stud; 5. Assembly cylinder; 6. Threaded hole; 7. Stud; 8. Screwing groove; 9. Movable plate; 10. Spring; 11. Stopper; 12. Stopper hole; 13. Reagent bottle; 14. Placing rack; 15. Rubber block; 16. Extrusion column. Detailed implementation manners

[0019] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0020] Embodiment

[0021] Please refer to Figures 1 to 4, the present utility model provides a technical solution: an induced pluripotent stem cell marker detection kit, including a box body 1, a box flip cover 2 is connected to the box body 1, a placement rack 14 is arranged inside the box body 1, reagent bottles 13 are inserted on the placement rack 14, a lock hole 3 is opened on the surface of the box flip cover 2, a lock post 4 is arranged inside the lock hole 3, the lock post 4 is fixed on the surface of the box body 1, an assembly cylinder 5 is inlaid and fixed on the surface of the box flip cover 2, an extrusion column 16 is slidably connected inside the assembly cylinder 5. Through the designed assembly cylinder 5, extrusion column 16 and spring 10, the assembly cylinder 5 containing the extrusion column 16 and spring 10 is installed on the box flip cover 2. The reagent bottles 13 are placed in the placement rack 14 inside the box body 1, the box flip cover 2 is covered, and the bent movable end of the box flip cover 2 is adjusted so that the lock hole 3 is stuck on the lock post 4 to fix the box flip cover 2. At the same time, the extrusion column 16 is placed on the top of the corresponding reagent bottle 13, and the spring 10 exerts an elastic force on the extrusion column 16 to push the extrusion column 16 to press tightly on the reagent bottle 13, realizing the fixation of the reagent bottles 13 inside the box, preventing the reagent bottles 13 from shaking randomly during transportation inside the box and easily causing the bottle body to collide and be damaged, resulting in the leakage of the reagent liquid. The bottom end of the extrusion column 16 is at the top of the reagent bottle 13, a spring 10 is arranged at the top end of the extrusion column 16, and the spring 10 is inside the assembly cylinder 5. A blocking hole 12 is opened on the side wall of the extrusion column 16, a blocking block 11 is arranged inside the blocking hole 12, and the blocking block 11 is fixed on the inner wall of the assembly cylinder 5. Through the designed blocking hole 12 and blocking block 11, the extrusion column 16 is limited to prevent the extrusion column 16 from detaching from the assembly cylinder 5. The cross-sections of the extrusion column 16 and the assembly cylinder 5 are both circular structures, and the central axes of the extrusion column 16 and the assembly cylinder 5 coincide with each other. A rubber block 15 is fixed on the bottom surface of the extrusion column 16. Through the designed rubber block 15, a soft contact buffering effect is achieved, avoiding the reagent bottle being easily damaged when the extrusion column 16 directly presses and fixes the reagent bottle. The cross-section of the rubber block 15 is a circular structure.

[0022] In this embodiment, preferably, a threaded hole 6 is opened on the top surface of the assembly cylinder 5, a stud 7 is threadedly connected inside the threaded hole 6, and one end of the stud 7 is connected with a movable plate 9. Through the designed threaded hole 6, stud 7 and movable plate 9, the stud 7 is rotated, and the stud 7 and the threaded hole 6 are threadedly connected and adjusted to drive the movable plate 9 to squeeze the spring 10 and adjust its compression deformation degree to achieve the best elastic extrusion effect. And the movable plate 9 is at one end of the spring 10. The cross-sections of the movable plate 9 and the stud 7 are both circular structures, and the central axes of the movable plate 9 and the stud 7 coincide with each other. A screwing groove 8 is opened on the top surface of the stud 7, and the cross-section of the screwing groove 8 is a hexagonal structure. Through the designed screwing groove 8, it is convenient for a tool to be inserted into the groove to rotate and adjust the stud 7.

[0023] Working principle and usage process of the utility model: Tools of the utility model are inserted into the screwing groove 8 to screw the stud 7. The stud 7 is threadedly connected with the threaded hole 6 for adjustment, driving the movable plate 9 to squeeze the spring 10 and adjusting its compression deformation degree to achieve the best elastic extrusion effect. The reagent bottle 13 is placed in the placement rack 14 in the box body 1, the box flip cover 2 is covered, and the bent movable end of the box flip cover 2 is adjusted so that its lock hole 3 is stuck on the lock post 4 to fix the box flip cover 2. At the same time, the extrusion post 16 is placed on the top of the corresponding reagent bottle 13, and the spring 10 applies an elastic force to the extrusion post 16 to push the extrusion post 16 to press tightly on the reagent bottle 13, realizing the fixation of the reagent bottle 13 in the box and preventing the reagent bottle 13 from shaking arbitrarily during transportation in the box, which is likely to cause the collision and damage of the bottle body and lead to the leakage of the reagent liquid.

[0024] Although the embodiments of the present utility model have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. An induced pluripotent stem cell marker detection kit, comprising a box body (1), a box flip cover (2) is connected to the box body (1), a placement rack (14) is arranged inside the box body (1), reagent bottles (13) are inserted on the placement rack (14), a lock hole (3) is formed on the surface of the box flip cover (2), a lock post (4) is arranged inside the lock hole (3), and the lock post (4) is fixed on the surface of the box body (1), characterized in that: An assembly cylinder (5) is fixedly inlaid on the surface of the box flip cover (2). An extrusion column (16) is connected inside the assembly cylinder (5) in a sliding manner. The bottom end of the extrusion column (16) is located at the top of the reagent bottle (13). A spring (10) is arranged at the top end of the extrusion column (16), and the spring (10) is inside the assembly cylinder (5).

2. The induced pluripotent stem cell marker detection kit according to claim 1, characterized in that: A retaining hole (12) is formed in the side wall of the extrusion column (16). A retaining block (11) is arranged inside the retaining hole (12), and the retaining block (11) is fixed on the inner wall of the assembly cylinder (5).

3. The detection kit for induced pluripotent stem cell markers according to claim 2, wherein: The cross-sections of the extrusion column (16) and the assembly cylinder (5) are both circular structures, and the central axes of the extrusion column (16) and the assembly cylinder (5) coincide with each other.

4. The detection kit for induced pluripotent stem cell markers according to claim 3, wherein: A rubber block (15) is fixed on the bottom end surface of the extrusion column (16), and the cross-section of the rubber block (15) is a circular structure.

5. The detection kit for induced pluripotent stem cell markers according to claim 1, wherein: A threaded hole (6) is formed in the top end surface of the assembly cylinder (5). A stud (7) is connected inside the threaded hole (6) by means of a thread. One end of the stud (7) is connected to a movable plate (9), and the movable plate (9) is located at one end of the spring (10).

6. The detection kit for induced pluripotent stem cell markers according to claim 5, wherein: The cross-sections of the movable plate (9) and the stud (7) are both circular structures, and the central axes of the movable plate (9) and the stud (7) coincide with each other.

7. The detection kit for induced pluripotent stem cell markers according to claim 6, wherein: A screwing groove (8) is formed in the top end surface of the stud (7), and the cross-section of the screwing groove (8) is a hexagonal structure.

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