Stem cell resuscitation water bath frame
Through the design of lifting and shaking components, the problem of the frozen storage tube not being able to shake when placed in the water bath is solved, and the efficient recovery of the frozen storage tube is achieved.
Patent Information
- Application Number
- CN202421890622.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-06
- Publication Date
- 2025-07-08
- Estimated Expiration
- 2034-08-06
AI Technical Summary
In the prior art, the frozen storage tube cannot be shaken at the same time when it is placed in the water bath, resulting in a long recovery time.
A stem cell resuscitation water bath rack is designed, including lifting components and shaking components. By adjusting the height of the frame and shaking the frozen storage tube, convenient height matching and shaking operations are achieved.
Improve the resuscitation efficiency of frozen storage tubes in water bath and shorten the recovery time.
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Figure CN223074191U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of stem cell water bath, and particularly relates to a stem cell resuscitation water bath rack. Background Art
[0002] Cell resuscitation refers to the process of thawing cells frozen in liquid nitrogen or a -70°C liquid nitrogen tank and then culturing them again so that the cells resume growth. Cell resuscitation operations require taking out the cryopreservation tube from the liquid nitrogen tank, putting it into a 37°C water bath, and shaking it from time to time to completely melt it within 1 minute, and then taking out the cells aseptically and putting them into the prepared culture medium. In order to perform resuscitation in batches, a bracket for cryopreservation tubes is often placed in a water bath device. For example, Chinese Patent No. 202122876580.9 discloses a cell cryopreservation tube water bath resuscitation device, which includes a bracket main body, limiting holes, limiting grooves, and a bracket base. On the inner walls of the bottom ends on both sides of the bracket main body, there are two groups of limiting grooves, and limiting holes are arranged inside the limiting grooves. A cryopreservation tube hole rack is arranged inside the bracket main body.
[0003] For the above-mentioned existing technology, there are still the following problems: Since the height of the rack is fixed and the structure only has a basic supporting function, it is not convenient to shake the rack and the cryopreservation tubes on it, resulting in a relatively long resuscitation time. Therefore, how to shake while performing water bath support for multiple cryopreservation tubes is a technical problem that needs to be solved. Summary of the Utility Model
[0004] The purpose of the utility model is to overcome the above technical deficiencies, and propose a stem cell resuscitation water bath rack to solve the technical problem of how to shake while performing water bath support for multiple cryopreservation tubes in the existing technology.
[0005] To achieve the above technical purpose, the utility model adopts the following technical solutions:
[0006] The utility model provides a stem cell resuscitation water bath rack, including:
[0007] A base;
[0008] A lifting assembly installed on the top of the base;
[0009] A shaking assembly, which includes a connecting piece, a locking piece, and a handheld part. The connecting piece is rotatably connected to the movable end of the lifting assembly. The locking piece is arranged on the connecting piece and is used to lock and unlock the relative rotation between the connecting piece and the movable end of the lifting assembly. The handheld part is arranged on the top of the connecting piece; and
[0010] A rack arranged on the connecting piece. A number of jacks are arranged in an array on the rack for inserting cryopreservation tubes.
[0011] In some embodiments, the lifting assembly includes a rotating head, a screw rod, a slider, and a guiding member. The screw rod is rotatably connected to the base and is threadedly connected to the slider. The guiding member is disposed on the base and is slidably connected to the slider for limiting and guiding the slider. The rotating head is disposed at the top end of the screw rod.
[0012] In some embodiments, the connecting member includes a base and a rotating shaft. The rotating shaft is disposed on one side of the base, penetrates into the interior of the slider, and is rotatably connected to the slider.
[0013] In some embodiments, the locking member includes an elastic telescopic structure, a lifting rod, and a plug pin. The elastic telescopic structure is mounted on the base. Both the lifting rod and the plug pin are connected to the movable end of the elastic telescopic structure. A through hole is formed at the top of the slider, and a limiting hole corresponding to the position of the through hole is formed at the top of the rotating shaft. The bottom end of the plug pin is aligned with the limiting hole and has a position state of inserting into the limiting hole and the through hole, and a position state of disengaging from the limiting hole and the through hole.
[0014] In some embodiments, the elastic telescopic structure includes a sleeve, a spring, a rod body, and a limiting ring. The rod body is slidably connected to the inside of the sleeve, and its two ends respectively penetrate through the top end and the bottom end of the sleeve. The limiting ring is disposed on the outside of the rod body and is connected to the bottom end of the spring. The top end of the spring is connected to the inner top wall of the sleeve. The lifting rod is disposed at the top end of the rod body, and the plug pin is disposed at the bottom end of the rod body.
[0015] In some embodiments, a stabilizing frame assembly is disposed at the bottom of the frame body for sleeving between the outsides of a plurality of cryogenic tubes.
[0016] In some embodiments, the stabilizing frame assembly includes a layer rack, a column rod, and a nut. The nut is threadedly connected to the column rod. The two nuts respectively abut against the bottom and the top of the layer rack. A plurality of alignment holes are formed on the layer rack, and the plurality of alignment holes correspond to the plurality of jacks one by one.
[0017] In some embodiments, a limiting rod is disposed on the movable end of the lifting assembly for limiting the swing amplitude of the shaking assembly.
[0018] In some embodiments, a limiting cover is inserted at the top of the frame body. Slots are formed on both sides of the frame body. L-shaped convex edges are disposed on both sides of the limiting cover, and the L-shaped convex edges are inserted into the slots.
[0019] In some embodiments, the limiting cover is a transparent cover body.
[0020] Compared with the prior art, the stem cell resuscitation water bath rack provided by the utility model adjusts the height position of the rack body directly through the lifting assembly, and can conveniently adjust the placement height of the cryopreservation tubes thereon according to the actual water bath depth, so that they are fully immersed in the water bath, but the tube caps are not submerged in the water bath. Moreover, the whole rack body can be shaken through the shaking assembly, so as to form a shaking action on the cryopreservation tubes, which is convenient to operate and improves the efficiency of water bath resuscitation. Brief Description of the Drawings
[0021] Figure 1 is a schematic structural diagram of the stem cell resuscitation water bath rack provided by an embodiment of the utility model;
[0022] Figure 2 is a schematic structural diagram of another perspective of the stem cell resuscitation water bath rack provided by an embodiment of the utility model;
[0023] Figure 3 is a partial front sectional view of the stem cell resuscitation water bath rack provided by an embodiment of the utility model;
[0024] Figure 4 is a three-dimensional view of the stem cell resuscitation water bath rack provided by an embodiment of the utility model with a limit cover thereon;
[0025] Figure 5 is an exploded three-dimensional view of the stem cell resuscitation water bath rack provided by an embodiment of the utility model with a limit cover thereon;
[0026] Figure 6 is a three-dimensional view of the stem cell resuscitation water bath rack provided by an embodiment of the utility model at an elevation angle;
[0027] Figure 7 is the utility model Figure 6 Partial enlarged view of part A.
[0028] Explanation of the reference numerals:
[0029] 1. Base;
[0030] 2. Lifting assembly; 21. Rotating head; 22. Screw rod; 23. Slide block; 24. Guide member; 2301. Through hole; 201. Limit rod;
[0031] 3. Shaking assembly; 31. Connecting member; 32. Locking member; 33. Holding part; 311. Base; 312. Rotating shaft; 321. Elastic telescopic structure; 322. Pull rod; 323. Plug; 3211. Sleeve; 3212. Spring; 3213. Rod body; 3214. Limit ring; 301. Limit hole;
[0032] 4. Rack body; 401. Insertion hole; 402. Slot;
[0033] 5. Stabilizer assembly; 51. Shelf; 52. Column rod; 53. Nut; 501. Alignment hole;
[0034] 6. Limit cover; 601. L-shaped flange. Detailed implementation manner
[0035] In order to make the purpose, technical solution and advantages of the present utility model clearer, the following further details the present utility model in conjunction with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.
[0036] In order to solve the technical problem of how to shake while supporting and placing multiple cryotubes in a water bath, the present utility model provides a stem cell resuscitation water bath rack, which can be conveniently adjusted according to the actual water bath depth, and is convenient for shaking the cryotubes to accelerate water bath resuscitation.
[0037] It should be noted that the stem cell resuscitation water bath rack described in the present utility model is used for but not limited to stem cell water bath resuscitation, etc. For the convenience of description, in the present utility model, only the application of the stem cell resuscitation water bath rack to stem cell water bath resuscitation is taken as an example for description, and the principle of the stem cell resuscitation water bath rack applied to other types of equipment is substantially the same as that applied to other cell water bath resuscitations, and will not be elaborated here one by one.
[0038] Please refer to Figure 1 , Figure 1 which is a schematic structural diagram of the stem cell resuscitation water bath rack in an embodiment of the present utility model. The stem cell resuscitation water bath rack includes a base 1, a lifting assembly 2, a shaking assembly 3, and a rack body 4; the lifting assembly 2 is installed on the top of the base 1 and is used for height adjustment of the rack body 4 and the shaking assembly 3. It forms the overall water bath rack with the base 1, the shaking assembly 3, and the rack body 4. Only by adjusting the height position of the rack body 4, the corresponding water bath depth can be matched, and there is no need for additional padding or replacing a new water bath rack, and the operation is more convenient.
[0039] Specifically, please refer to Figure 1 , the lifting assembly 2 includes a rotating head 21, a screw rod 22, a slider 23, and a guiding member 24. The screw rod 22 is rotatably connected to the base 1 and is threadedly connected to the slider 23. The guiding member 24 is disposed on the base 1 and is slidably connected to the slider 23 for limiting and guiding the slider 23. The rotating head 21 is disposed at the top of the screw rod 22. By rotating the rotating head 21, under the rotation of the screw rod 22, the slider 23 slides up and down along the guiding member 24, and the effect of lifting adjustment can be achieved. Just stop adjusting to the corresponding water bath height.
[0040] Among them, the guiding member 24 can adopt a semi - enclosed housing, which is attached to the two side walls of the slider 23 for limiting and guiding it. It can be understood that the guiding member 24 can also adopt a straight rod passing through the slider 23 to achieve the specific guiding effect.
[0041] In this embodiment, please refer to Figure 1 , in order to shake the cryotube while placing it, and improve its resuscitation speed, a shaking assembly 3 is arranged between the lifting assembly 2 and the frame body 4. Among them, the shaking assembly 3 includes a connecting member 31, a locking member 32 and a hand - holding part 33. The connecting member 31 is rotatably connected to the movable end of the lifting assembly 2. The locking member 32 is arranged on the connecting member 31 and is used to lock and unlock the relative rotation between the connecting member 31 and the movable end of the lifting assembly 2. The hand - holding part 33 is arranged at the top of the connecting member 31, and the hand - holding part 33 is in the shape of a ring - shaped handle for hand - holding. When operating the locking member 32 to unlock the rotational locking between the connecting member 31 and the movable end of the lifting assembly 2, shaking the hand - holding part 33 can drive the connecting member 31 to rotate, thereby driving the frame body 4 to rotate, forming a shaking action on the cryotubes on the frame body 4. When the locking member 32 locks the rotational connection between the connecting member 31 and the movable end of the lifting assembly 2, the entire water - bath rack can be stabilized for lifting or transferring, and for taking or placing cryotubes.
[0042] Furthermore, the frame body 4 is arranged on the connecting member 31, and a plurality of jacks 401 are arranged in an array on the frame body 4 for inserting cryotubes.
[0043] In one of the embodiments, please refer to Figure 2 and Figure 3 , the connecting member 31 includes a base 311 and a rotating shaft 312. The rotating shaft 312 is arranged on one side of the base 311, penetrates into the interior of the slider 23, and is rotatably connected to the slider 23. The base 311 is detachably connected to the frame body 4.
[0044] In this embodiment, the frame body 4 and the base 311 can be installed by a detachable connection method, which is convenient for installation and disassembly, such as snap - connection, bolt - connection, etc.
[0045] Furthermore, in order to lock and unlock the rotation of the rotating shaft 312, please refer to Figure 3, the locking member 32 includes an elastic telescopic structure 321, a lifting rod 322 and a bolt 323. The elastic telescopic structure 321 is installed on the base 311. Both the lifting rod 322 and the bolt 323 are connected to the movable end of the elastic telescopic structure 321. By pulling the lifting rod 322, the movable end of the elastic telescopic structure 321 is telescoped, thereby driving the bolt 323 to move. A through hole 2301 is provided at the top of the slider 23, and a limiting hole 301 corresponding to the position of the through hole is provided at the top of the rotating shaft 312. The bottom end of the bolt 323 is aligned with the limiting hole 301, and has a position state of inserting into the limiting hole 301 and the through hole 2301, and a position state of disengaging from the limiting hole 301 and the through hole 2301. When the limiting hole 301 is aligned with the through hole 2301 and the bolt 323 penetrates through the limiting hole 301 and the through hole 2301, the rotation limit of the rotating shaft 312 is formed. On the contrary, when pulling the lifting rod 322 to drive the movable end of the elastic telescopic structure 321 to disengage the bolt 323 from the limiting hole 301 and the through hole 2301, the rotation limit of the rotating shaft 312 can be released.
[0046] Specifically, in order to achieve the effect of elastic telescoping, please refer to Figure 2 and Figure 3 , the elastic telescopic structure 321 includes a sleeve 3211, a spring 3212, a rod body 3213 and a limiting ring 3214. The rod body 3213 is slidably connected to the inside of the sleeve 3211, and both ends thereof penetrate through the top end and the bottom end of the sleeve 3211 respectively. The limiting ring 3214 is arranged on the outer side of the rod body 3213 and is connected to the bottom end of the spring 3212. The top end of the spring 3212 is connected to the inner top wall of the sleeve 3211. The lifting rod 322 is arranged at the top end of the rod body 3213, and the bolt 323 is arranged at the bottom end of the rod body 3213. When pulling the lifting rod 322, the rod body 3213 is driven, so that the limiting ring 3214 compresses the spring 3212 upward, thereby lifting the bolt 323. On the contrary, when the lifting rod 322 is released, under the rebound elongation of the spring 3212, the limiting ring 3214 is pushed to move the rod body 3213 downward and insert the bolt 323 downward.
[0047] In one embodiment, in order to improve the stability of placing the cryotubes, please refer to Figure 4 and Figure 6 , a stabilizing frame assembly 5 is provided at the bottom of the frame body 4, which is used to be sleeved outside a plurality of cryotubes, and together with the frame body 4 forms a two-layer plate frame for inserting the cryotubes.
[0048] Specifically, the stabilizing frame assembly 5 includes a shelf 51, a column rod 52, and a nut 53. The nut 53 is threadedly connected to the column rod 52. The two nuts 53 respectively abut against the bottom and top of the shelf 51. A plurality of alignment holes 501 are formed in the shelf 51. The plurality of alignment holes 501 correspond to the plurality of insertion holes 401 one by one. The cryotubes are inserted into the vertically aligned alignment holes 501 and insertion holes 401. By rotating the nut 53, the height position of the shelf 51 can be adjusted. By tightening the upper and lower nuts 53 to clamp the shelf 51, the position can be fixed.
[0049] In one embodiment, in order to prevent excessive shaking from causing the test tubes to be submerged in the water bath, please refer to Figure 7 , a limiting rod 201 is provided on the movable end of the lifting assembly 2 to limit the swinging amplitude of the shaking assembly 3. Among them, a bump extends downward from the base 311 of the shaking assembly 3. The number of limiting rods 201 is two, which are respectively located on the left and right sides of the bump. When rotating, it serves to limit the rotation angle of the base 311, thereby achieving the effect of limiting the shaking amplitude of the entire shaking assembly 3.
[0050] In one embodiment, in order to prevent the test tubes from floating in the water bath, please refer to Figure 4 and Figure 5 , a limiting cover 6 is inserted at the top of the frame body 4. Slots 402 are formed on both sides of the frame body 4. L-shaped convex edges 601 are provided on both sides of the limiting cover 6. The L-shaped convex edges 601 are inserted into the slots 402. By means of sliding insertion, the limiting cover 6 is covered on the top of the frame body 4 to limit the top of the cryotube, thereby preventing it from floating in the water bath.
[0051] Furthermore, the limiting cover 6 is a transparent cover body, which is convenient for observing the situation of the cryotubes.
[0052] For a better understanding of the present invention, the technical solutions of the present invention will be described in detail below in conjunction with Figures 1 to 7 : Rotate the rotating head 21 to adjust the lifting of the slider 23, and adjust the height positions of the shaking assembly 3 and the frame body 4 to match the water bath depth of the corresponding water bath device. Place it in the water bath tank of the water bath device. Hold the holding part 33 by hand, pull up the lifting rod 322 with fingers, and separate the pin 323 from the limiting hole 301 and the through hole 2301, that is, rotate the connecting piece 31, drive the frame body 4 and the cryotubes thereon to shake. After shaking, turn it back to the initial position, release the lifting rod 322, and under the elastic push of the spring 3212, the pin 323 penetrates the through hole 2301 and is inserted into the limiting hole 301 to form a lock, and then hold the holding part 33 to transfer the entire water bath rack.
[0053] The specific embodiments of the present utility model described above do not constitute a limitation on the protection scope of the present utility model. Any other corresponding changes and deformations made according to the technical concept of the present utility model shall be included within the protection scope of the claims of the present utility model.
Claims
1. A stem cell resuscitation water bath rack, characterized in that, Comprising: Base; Lifting assembly, which is installed on the top of the base; Shaking assembly, which includes a connecting piece, a locking piece and a handheld part. The connecting piece is rotatably connected to the movable end of the lifting assembly. The locking piece is arranged on the connecting piece and is used to lock and unlock the relative rotation between the connecting piece and the movable end of the lifting assembly. The handheld part is arranged on the top of the connecting piece; And Frame body, which is arranged on the connecting piece. A plurality of jacks are arranged in an array on the frame body for inserting cryopreservation tubes.
2. The stem cell resuscitation water bath rack according to claim 1, characterized in that The lifting assembly includes a rotating head, a screw rod, a slider and a guiding piece. The screw rod is rotatably connected to the base and is threadedly connected to the slider. The guiding piece is arranged on the base and is slidably connected to the slider for limiting and guiding the slider. The rotating head is arranged at the top end of the screw rod.
3. The stem cell resuscitation water bath rack according to claim 2, wherein The connecting piece includes a base and a rotating shaft. The rotating shaft is arranged on one side of the base, penetrates into the inside of the slider and is rotatably connected to the slider.
4. The stem cell resuscitation water bath rack according to claim 3, characterized in that, The locking piece includes an elastic telescopic structure, a lifting rod and a plug pin. The elastic telescopic structure is installed on the base. The lifting rod and the plug pin are both connected to the movable end of the elastic telescopic structure. A through hole is opened at the top of the slider, and a limiting hole corresponding to the position of the through hole is opened at the top of the rotating shaft. The bottom end of the plug pin is aligned with the limiting hole and has a position state of inserting into the limiting hole and the through hole, and a position state of disengaging from the limiting hole and the through hole.
5. The stem cell resuscitation water bath rack according to claim 4, wherein The elastic telescopic structure includes a sleeve, a spring, a rod body and a limiting ring. The rod body is slidably connected inside the sleeve, and its two ends respectively penetrate through the top end and the bottom end of the sleeve. The limiting ring is arranged on the outside of the rod body and is connected to the bottom end of the spring. The top end of the spring is connected to the inner top wall of the sleeve. The lifting rod is arranged at the top end of the rod body, and the plug pin is arranged at the bottom end of the rod body.
6. The stem cell resuscitation water bath rack according to claim 5, wherein, A stabilizing frame assembly is arranged at the bottom of the frame body for sleeving outside multiple cryopreservation tubes.
7. The stem cell resuscitation water bath rack according to claim 6, wherein The stabilizing frame assembly includes a shelf, a column rod and a nut. The nut is threadedly connected to the column rod. The two nuts respectively abut against the bottom and the top of the shelf. A plurality of alignment holes are opened on the shelf, and the plurality of alignment holes correspond to the plurality of jacks one by one.
8. The stem cell resuscitation water bath rack according to claim 7, wherein A limiting rod is arranged on the movable end of the lifting assembly for restricting the swinging amplitude of the shaking assembly.
9. The stem cell resuscitation water bath rack according to claim 8, wherein A limiting cover is inserted at the top of the frame body. Slots are opened on both sides of the frame body. L-shaped convex edges are arranged on both sides of the limiting cover, and the L-shaped convex edges are inserted into the slots.
10. The stem cell resuscitation water bath rack according to claim 9, wherein The limiting cover is a transparent cover body.
Citation Information
Patent Citations
Cell cryopreservation tube water bath resuscitation device
CN216688163U