Cell culture device for evaluating cell migration behavior

Abstract

The utility model relates to the field of cell culture devices, in particular to a cell culture device for evaluating cell migration behaviors. The utility model provides the cell culture device for evaluating the cell migration behavior, which can add a nutrient solution into the sample table without opening the top cover and prevent external bacteria from entering the sample table to influence the normal cell migration behavior. A cell culture device for evaluating cell migration behaviors comprises a cylinder body, a top cover, fixing screws and the like, the top cover is connected to the upper side of the cylinder body in a clamped mode, and the fixing screws are connected to the inner side of the cylinder body in a threaded mode. According to the utility model, the handle is pressed to move downwards, and the spring is extruded and contracted to drive the connecting frame to move downwards, so that the spray head moves downwards to spray a mixed nutrient solution into the sample table, and the nutrient solution can be added into the sample table under the condition that the top cover is not opened; external bacteria are prevented from entering the sample table to influence the normal migration behavior of the cells.

Description

Technical Field

[0001] The utility model relates to the field of cell culture devices, in particular to a cell culture device for evaluating cell migration behavior. Background Art

[0002] Cell migration, also known as cell crawling, cell movement or cell locomotion, refers to the movement of cells after receiving migration signals or sensing gradients of certain substances. When evaluating cell migration behavior, it is usually carried out on a cell culture device.

[0003] In the existing cell culture device for evaluating cell migration behavior, cells are cultured in the culture solution in a culture barrel to facilitate the evaluation of cell migration behavior. However, since nutrient solution needs to be added when culturing cells, and the top cover needs to be opened when adding the nutrient solution, it may cause external bacteria to enter the culture solution, affecting the cell migration behavior.

[0004] Therefore, a cell culture device for evaluating cell migration behavior has now been developed, which can add nutrient solution to the sample table without opening the top cover, avoiding external bacteria from entering the sample table and affecting the normal cell migration behavior. Summary of the Utility Model

[0005] In order to overcome the defect that in the existing cell culture device for evaluating cell migration behavior, the top cover needs to be opened when adding nutrient solution, which may cause external bacteria to enter the culture solution and affect the cell migration behavior, the utility model provides a cell culture device for evaluating cell migration behavior, which can add nutrient solution to the sample table without opening the top cover, avoiding external bacteria from entering the sample table and affecting the normal cell migration behavior.

[0006] Technical Solution: A cell culture device for evaluating cell migration behavior includes a cylinder body, a top cover, fixing screws, a sample table and a feeding mechanism. The top cover is clamped on the upper side of the cylinder body. A plurality of fixing screws are connected to the inner side of the cylinder body in a threaded manner. The sample tables are clamped on the fixing screws, and the sample tables are all in contact with the cylinder body. The top cover is provided with a feeding mechanism capable of adding nutrient solution to the sample table.

[0007] In addition, particularly preferably, a placement groove is formed in the sample table.

[0008] In addition, particularly preferably, the feeding mechanism includes a nutrient frame, a spring, a handle, a mixing frame, a nozzle and a connecting frame. Two left and right nutrient frames are clamped on the upper side of the top cover. The handle is slidably connected to the middle of the top cover. A spring is connected between the handle and the top cover. The mixing frame is connected to the lower side of the top cover. The nutrient frames are all connected to the mixing frame. A plurality of nozzles are slidably connected to the lower side of the mixing frame. The lower part of the handle is connected with a connecting frame, and the nozzles are all connected to the connecting frame.

[0009] In addition, it is particularly preferred that a soft pad is provided on the handle.

[0010] In addition, it is particularly preferred that a filter screen is provided on the nozzle.

[0011] In addition, it is particularly preferred that a splicing mechanism is further included. The splicing mechanism includes a connecting block and a connecting rod. A plurality of connecting blocks are connected to the outside of the barrel body, and connecting rods are connected between two adjacent connecting blocks in the upper and lower parts.

[0012] Compared with the prior art, the present utility model has the following advantages: 1. By pressing the handle to move downward, the spring is compressed and contracted, driving the connecting frame to move downward, so that the nozzle moves downward to spray the mixed nutrient solution onto the sample table, achieving the effect of being able to add nutrient solution to the sample table without opening the top cover and avoiding external bacteria from entering the sample table and affecting the normal migration behavior of cells.

[0013] 2. By the mutual clamping and cooperation of the connecting blocks, the barrel bodies are spliced with each other, and the connecting rods strengthen the stability of the splicing, achieving the effect of being able to splice the barrel bodies and facilitating the improvement of the placement stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a three-dimensional structural schematic diagram of the present utility model.

[0015] Figure 2 It is a three-dimensional structural sectional schematic diagram of a part of the present utility model.

[0016] Figure 3 It is a three-dimensional structural sectional schematic diagram of the blanking mechanism and the splicing mechanism of the present utility model.

[0017] Figure 4 It is a partial three-dimensional structural sectional schematic diagram of the blanking mechanism of the present utility model.

[0018] Among them, the above-mentioned drawings include the following reference numerals: 1. Barrel body, 2. Top cover, 3. Fixing screw, 4. Sample table, 5. Blanking mechanism, 51. Nutrient frame, 52. Spring, 53. Handle, 54. Mixing frame, 55. Nozzle, 56. Connecting frame, 6. Splicing mechanism, 61. Connecting block, 62. Connecting rod. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] To make the purpose, technical solution and advantages of the present utility model clearer and more understandable, the present utility model will be further described in detail below in conjunction with the specific embodiments and with reference to the accompanying drawings. It should be understood that these descriptions are exemplary and are not intended to limit the scope of the present utility model. In addition, in the following description, the descriptions of well-known structures and technologies are omitted to avoid unnecessarily confusing the concepts of the present utility model.

[0020] A cell culture device for evaluating cell migration behavior, as Figure 1 and Figure 2 shown, comprising a barrel body 1, a top cover 2, fixing screws 3, a sample stage 4 and a feeding mechanism 5. The top cover 2 is snap-connected to the upper side of the barrel body 1. A plurality of fixing screws 3 are threadedly connected to the inner side of the barrel body 1. The sample stages 4 are snap-connected to the fixing screws 3. A placement groove is formed on the sample stage 4 to facilitate cell culture. The sample stages 4 are all in contact with the barrel body 1. The feeding mechanism 5 is provided on the top cover 2.

[0021] As Figure 1 and Figure 3 shown, the feeding mechanism 5 comprises a nutrient frame 51, a spring 52, a handle 53, a mixing frame 54, a nozzle 55 and a connecting frame 56. Two left and right nutrient frames 51 are snap-connected to the upper side of the top cover 2. The handle 53 is slidably connected to the middle of the top cover 2. A soft pad is provided on the handle 53 for easy grasping. A spring 52 is connected between the handle 53 and the top cover 2. The mixing frame 54 is connected to the lower side of the top cover 2. The nutrient frames 51 are all connected to the mixing frame 54. Four nozzles 55 are slidably connected to the lower side of the mixing frame 54. A filter screen is provided on the nozzle 55 to facilitate filtering of the nutrient solution. The lower part of the handle 53 is connected to the connecting frame 56. The nozzles 55 are all connected to the connecting frame 56.

[0022] When using the present utility model, first place the barrel body 1 in the cell culture area, fix the sample stage 4 inside the barrel body 1 through the fixing screws 3, then place the cells for evaluating cell migration behavior on the sample stage 4 and culture them with a culture solution. After that, snap-fit the top cover 2 with the barrel body 1 so that the top cover 2 closes the barrel body 1 to facilitate sealed culture of the cells and prevent external bacteria from entering the cells. After the cell culture is completed, the cell migration behavior can be evaluated. When it is necessary to add a nutrient solution to the cells, the two nutrient solutions can be respectively poured into the nutrient frames 51 so that the nutrient solutions flow into the mixing frame 54 for mixing. After mixing is completed, press the handle 53 to move downward, the spring 52 is compressed and contracted, driving the connecting frame 56 to move downward, so that the nozzles 55 move downward to spray the mixed nutrient solution into the sample stage 4, thus playing the role of being able to add the nutrient solution to the sample stage 4 without opening the top cover 2 and preventing external bacteria from entering the sample stage 4 and affecting the normal migration behavior of the cells. After spraying, release the handle 53 to make the spring 52 rebound and drive the nozzles 55 to reset.

[0023] As Figure 1 and Figure 4 shown, it further comprises a splicing mechanism 6. The splicing mechanism 6 comprises a connecting block 61 and a connecting rod 62. Twelve connecting blocks 61 are connected to the outer side of the barrel body 1. Connecting rods 62 are connected between two adjacent connecting blocks 61 in the upper and lower parts.

[0024] Using the splicing mechanism 6 of the present device, the barrel body 1 can be spliced. Through the mutual clamping and cooperation of the connecting blocks 61, the barrel bodies 1 are spliced with each other, and the connecting rod 62 strengthens the stability of the splicing, thereby playing a role in being able to splice the barrel body 1 and facilitating the improvement of the placement stability.

[0025] It should be understood that this embodiment is only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of this application.

Claims

1. A cell culture device for evaluating cell migration behavior, characterized in that, It includes a cylinder body (1), a top cover (2), fixing screws (3), a sample table (4) and a blanking mechanism (5). The top cover (2) is snap-connected to the upper side of the cylinder body (1). A plurality of fixing screws (3) are connected to the inner side of the cylinder body (1) in a threaded manner. The sample table (4) is snap-connected to each of the fixing screws (3), and the sample tables (4) are all in contact with the cylinder body (1). A blanking mechanism (5) capable of adding nutrient solution to the sample table (4) is provided on the top cover (2).

2. The cell culture apparatus for evaluating cell migration behavior according to claim 1, characterized in that, A placement groove is provided on the sample table (4).

3. The cell culture apparatus for evaluating cell migration behavior according to claim 1, characterized in that, The blanking mechanism (5) includes a nutrient frame (51), a spring (52), a handle (53), a mixing frame (54), a nozzle (55) and a connecting frame (56). Two left and right nutrient frames (51) are snap-connected to the upper side of the top cover (2). A handle (53) is slidably connected to the middle of the top cover (2), and a spring (52) is connected between the handle (53) and the top cover (2). A mixing frame (54) is connected to the lower side of the top cover (2). The nutrient frames (51) are all connected to the mixing frame (54). A plurality of nozzles (55) are slidably connected to the lower side of the mixing frame (54). A connecting frame (56) is connected to the lower part of the handle (53), and the nozzles (55) are all connected to the connecting frame (56).

4. The cell culture device for evaluating cell migration behavior according to claim 3, characterized in that, A soft pad is provided on the handle (53).

5. The cell culture apparatus for evaluating cell migration behavior according to claim 3, characterized in that, A filter screen is provided on the nozzle (55).

6. The cell incubator for evaluating cell migration behavior according to claim 3, characterized in that, It further includes a splicing mechanism (6). The splicing mechanism (6) includes a connecting block (61) and a connecting rod (62). A plurality of connecting blocks (61) are connected to the outer side of the cylinder body (1), and connecting rods (62) are connected between two adjacent connecting blocks (61) in the upper and lower parts.

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