Allergen transgene specificity detection column

Abstract

The utility model provides an allergen transgene specificity detection column which comprises a shell, a water absorption layer is filled at the bottom in the shell, a detection layer is arranged on the water absorption layer, a detection antibody is arranged in the middle of the detection layer, a cap is arranged at the upper end of the shell, the middle of the cap is concave downwards and extends to the detection layer, and the detection antibody is arranged on the detection layer. According to the utility model, the simplified operation process allows a user to quickly complete detection, and the immunochromatographic test strip is suitable for on-site quick screening, simple to operate, free of professional equipment, convenient for non-professional personnel to use, capable of intuitively judging the detection result through the change of color or fluorescence, easy to observe and suitable for detection of various allergens or transgenic components; the method has a wide application scene.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to an allergen transgenic specific detection column. Background Art

[0002] With the acceleration of the modern life rhythm and the improvement of health awareness, it has become particularly important to quickly detect allergens and transgenic components in food. Traditional detection methods are time-consuming, costly, and complex to operate, and are not suitable for rapid on-site detection. With the increasing concern about food safety and personal health, the demand for rapid, simple, and accurate detection methods is growing.

[0003] Therefore, a rapid and simple detection means is needed to meet the requirements of on-site rapid screening. The detection method should have high sensitivity and high specificity to reduce misjudgment. The operation process needs to be simplified to adapt to the use of non-professionals. Summary of the Utility Model

[0004] The technical problem to be solved by the utility model is that the traditional detection method is time-consuming, costly, and complex to operate, and is not suitable for rapid on-site detection.

[0005] To solve the above technical problem, an allergen transgenic specific detection column provided by the utility model includes a housing. The bottom inside the housing is filled with a water-absorbing layer. A detection layer is arranged on the water-absorbing layer. A detection antibody is arranged in the middle of the detection layer. A cap is arranged at the upper end of the housing. The middle of the cap is concave downward and extends to the detection layer. Air holes are arranged on the outer side of the housing.

[0006] A technical solution provided by this application further has the following technical features:

[0007] Preferably, the housing is cylindrical or rectangular, and the housing is a plastic outer shell.

[0008] Preferably, the water-absorbing layer is filled with a water-absorbing material.

[0009] Preferably, the detection layer is a PVDF membrane.

[0010] Preferably, the detection antibody is an allergen antibody or a transgenic antibody.

[0011] Preferably, the fixed area of the detection antibody is a region with a diameter of 2 mm at the center of the detection layer.

[0012] Advantages of the Utility Model

[0013] 1. The simplified operation process of the present utility model allows users to quickly complete the detection, which is suitable for on-site rapid screening. The operation is simple and does not require professional equipment, facilitating the use by non-professional personnel. The uneven PVDF membrane surface increases the adsorption capacity of antibodies, improving the detection sensitivity. Using highly specific antibodies ensures the accuracy and reliability of the detection. The detection results can be visually judged through color or fluorescence changes, which are easy to observe. It is applicable to the detection of various allergens or genetically modified components and has a wide range of application scenarios.

[0014] 2. The present utility model effectively reduces non-specific binding through the use of multiple washing solutions, improving the specificity of the detection. According to different detection requirements, enzyme-labeled antibodies or fluorescently labeled antibodies can be selected for use. Compared with traditional detection methods, the detection column has high cost-effectiveness and is suitable for large-scale applications.

[0015] 3. The dry-state storage method of the detection column of the present utility model facilitates long-term preservation and transportation. Using a disposable plastic outer shell avoids cross-contamination and improves the safety of the detection. Whether it is color change or fluorescence signal, the judgment criteria for the results are clear, reducing the error of subjective interpretation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a three-dimensional view of a specific detection column for allergens and genetically modified components.

[0017] Figure 2 is a schematic structural diagram of a specific detection column for allergens and genetically modified components.

[0018] As shown in the figure:

[0019] 1. Housing; 2. Water-absorbing layer; 3. Detection layer; 4. Cap; 5. Air hole; 6. Detection antibody. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] The following will further illustrate the specific embodiments of the present utility model with reference to the accompanying drawings. Among them, the same components are denoted by the same reference numerals.

[0021] It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to the directions in the drawings, and the words "inner" and "outer" respectively refer to the directions towards or away from the geometric center of a specific component.

[0022] In order to make the content of the present utility model more clearly understood, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.

[0023] Embodiment 1

[0024] Combined with the attached Figure 1As shown in the figure, an allergen transgenic specific detection column includes a housing 1. The bottom of the housing 1 is filled with a water-absorbing layer 2. A detection layer 3 is provided on the water-absorbing layer 2. A detection antibody 6 is provided in the middle of the detection layer 3. A cap 4 is provided at the upper end of the housing 1. The middle of the cap 4 is concave downward and extends to the detection layer 3. The housing 1 is cylindrical or rectangular, and the housing 1 is a plastic outer shell. The detection antibody 6 is an allergen antibody or a transgenic antibody. The fixed area of the detection antibody 6 is a region with a diameter of 2 mm in the center of the detection layer 3.

[0025] The detection layer 3 is a PVDF membrane. As a solid-phase carrier, it fixes the detection antibody 6 for immunological reaction. It has good chemical stability, high mechanical strength, and an uneven surface to increase the antibody adsorption capacity. It is made by casting or spinning processes and the antibody is fixed in a specific area. It has the advantages of improving detection sensitivity, good biocompatibility and stability.

[0026] The detection antibody 6 specifically binds to the allergen or transgenic component in the sample. It has high specificity and affinity. The detection antibody 6 is obtained through antibody preparation technology and fixed in the central region of the PVDF membrane. It realizes the rapid qualitative detection of the target analyte.

[0027] Air holes 5 are provided on the outer side of the housing 1.

[0028] The air holes 5 allow air exchange between the inside and the outside environment of the housing 1, which helps to maintain the air pressure balance inside the detection column. When the sample and washing solution are added to the detection column, the air holes 5 have an appropriate pore size, which can adjust the flow rate of the liquid in the water-absorbing layer 2, preventing both the liquid from flowing too slowly and resulting in too long a detection time, and the liquid from flowing too fast and causing insufficient reaction. When adding the liquid, the air holes 5 can serve as exhaust holes to reduce the formation of bubbles and avoid liquid overflow from the detection column.

[0029] The air holes 5 can prevent the stability and activity of the detection antibody 6 from being affected due to too high humidity inside the housing 1. For the case of using enzyme-labeled antibodies, the air holes 5 can provide the necessary air permeability, which helps the contact between the substrate and the enzyme and promotes the development of the color reaction.

[0030] The cap 4 is used to cover the PVDF membrane, providing a reaction container to prevent the volatilization of the sample and reagents. It is manufactured by injection molding process. It has the advantages of facilitating the addition of the sample and reagents and simplifying the operation process.

[0031] The housing 1 provides the structural support of the detection column and protects the internal components. It is usually made of materials such as polypropylene or polyethylene, which are lightweight and have good chemical stability. It is manufactured by injection molding process. It has the advantage of protecting the internal water-absorbing material and detection membrane from the influence of external physical and chemical factors.

[0032] The water-absorbing layer 2 is filled with a water-absorbing material. The water-absorbing layer 2 can quickly absorb and evenly distribute the sample and the reagent. It has high water absorbency and can rapidly diffuse the liquid. High water-absorbing polymers or fiber materials can be used. It has the advantages of ensuring sufficient contact between the sample and the detection antibody 6 and improving the reaction efficiency.

[0033] The working process of the present utility model:

[0034] Add the extract of the sample to be tested into the cap 4 of the concave plastic of the detection column. The sample is absorbed by the water-absorbing material and diffuses along the PVDF membrane. The allergen or transgenic component in the sample specifically binds to the antibody immobilized on the PVDF membrane. Add the washing solution in portions to remove the non-specifically bound materials and reduce the background signal. Add the enzyme-labeled antibody or fluorescently labeled antibody of the allergen transgenic to further react with the bound antigen.

[0035] Color reaction:

[0036] Enzyme-labeled antibody: Add the TMB substrate. The positive sample reacts with the substrate, and the central area of the PVDF membrane shows blue.

[0037] Fluorescently labeled antibody: Use an ultraviolet lamp to irradiate, and the positive sample produces a fluorescent signal.

[0038] Result judgment: Judge whether the sample is positive or negative according to the color change or fluorescent signal of the PVDF membrane.

[0039] The present utility model has the following advantages:

[0040] 4. The simplified operation process of the present utility model allows users to quickly complete the detection, which is suitable for on-site rapid screening. The operation is simple, no professional equipment is required, and it is convenient for non-professional personnel to use. The uneven surface of the PVDF membrane increases the antibody adsorption capacity and improves the detection sensitivity. Using highly specific antibodies ensures the accuracy and reliability of the detection. The detection result can be intuitively judged through the color or fluorescence change, which is easy to observe. It is applicable to the detection of a variety of allergens or transgenic components and has a wide range of application scenarios.

[0041] 5. The present utility model effectively reduces non-specific binding through the use of multiple washing solutions, improving the specificity of the detection. According to different detection requirements, enzyme-labeled antibodies or fluorescently labeled antibodies can be selected. Compared with traditional detection methods, the detection column of the present utility model has high cost-effectiveness and is suitable for large-scale applications.

[0042] 6. The dry-state storage method of the detection column of the present utility model is convenient for long-term storage and transportation. Using a disposable plastic shell avoids cross-contamination and improves the safety of the detection. Whether it is color change or fluorescent signal, the judgment criteria for the results are clear, reducing the error of subjective interpretation.

[0043] Embodiment Two

[0044] Prepare the test strip: The test strip contains a water-absorbing material and a PVDF membrane, and a detection antibody 6 for allergens or genetically modified organisms is fixed in the central area of the PVDF membrane.

[0045] I. Add the sample to be tested: Take 50 μL of the extract of the sample to be tested and add it to the concave cover of the test strip.

[0046] II. Wait for absorption: Wait for the sample liquid to be completely absorbed by the PVDF membrane and the water-absorbing material.

[0047] III. Washing step: Add 100 μL of washing solution to the test strip and repeat this step 3 times to wash away the unbound sample.

[0048] IV. Add the antibody: After the washing solution is completely absorbed, add 50 μL of the enzyme-labeled antibody or fluorescently labeled antibody for allergens or genetically modified organisms.

[0049] V. Wait for absorption again: Wait for the enzyme-labeled antibody or fluorescently labeled antibody to be completely absorbed.

[0050] VI. Washing step again: Add 100 μL of washing solution again and repeat 3 times to remove the unspecifically bound labeled antibody.

[0051] VII. Color reaction (enzyme-labeled antibody): If an enzyme-labeled antibody is used, add 50 μL of TMB substrate. A positive sample will cause the PVDF membrane to turn blue, while a negative sample will keep the PVDF membrane white.

[0052] VIII. Fluorescence detection (fluorescently labeled antibody): If a fluorescently labeled antibody is used, irradiate the PVDF membrane with an ultraviolet lamp. A positive sample will produce fluorescence on the PVDF membrane, while a negative sample will have no fluorescence.

[0053] All the standard parts used in the present utility model can be purchased from the market. The special-shaped parts can be customized according to the description in the specification and the drawings. The specific connection methods of each part all adopt conventional means such as bolts, rivets, and welding that are mature in the prior art. The machines, parts, and equipment all adopt conventional models in the prior art. In addition, the circuit connection adopts the conventional connection method in the prior art, which will not be elaborated here. The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0054] The above describes the present utility model and its implementation manners. Such description is not restrictive. What is shown in the drawings is only one of the implementation manners of the present utility model, and the actual structure is not limited thereto. Generally speaking, if those skilled in the art are inspired by it and design, without creative work, a structural manner and an embodiment similar to the technical solution without departing from the creative purpose of the present utility model, they shall fall within the protection scope of the present utility model.

Claims

1. A specific detection column for allergen transgenes, comprising a housing, characterized in that: The bottom inside the housing is filled with a water-absorbing layer, a detection layer is arranged on the water-absorbing layer, a detection antibody is provided in the middle of the detection layer, a cap is arranged at the upper end of the housing, the middle of the cap is concave downward and extends to the detection layer, and air holes are arranged on the outer side of the housing.

2. The allergen transgenic specific detection column according to claim 1, wherein: The housing is cylindrical or rectangular, and the housing is a plastic shell.

3. The allergen transgenic specific detection column according to claim 1, characterized in that: The water-absorbing layer is filled with a water-absorbing material.

4. The allergen transgenic specific detection column according to claim 1, wherein: The detection layer is a PVDF membrane.

5. The allergen transgenic specific detection column according to claim 1, wherein: The detection antibody is an allergen antibody or a transgenic antibody.

6. The specific detection column for allergen transgene according to claim 1 or 5, characterized in that: The fixed area of the detection antibody is an area with a diameter of 2 mm in the center of the detection layer.

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