Magnetic stirring rod and stirring type suspension cell culture bottle

Abstract

The utility model provides a magnetic stirring rod and a stirring type suspension cell culture bottle, and the magnetic stirring rod comprises a fixed shaft, a magnetic stirring rod and a stirring type suspension cell culture bottle, the bottom of the fixed shaft is provided with a lower limiting ring; the connector comprises a lantern ring and an upper baffle ring which are concentrically arranged, and the upper baffle ring is a baffle ring arranged at the upper part of the outer wall of the lantern ring; the outer diameter of the lower limiting ring of the fixing shaft is larger than the inner diameter of the lantern ring. The lantern ring is arranged on the periphery of the fixing shaft in a sleeving mode, and the lower limiting ring of the fixing shaft is limited to the bottom end of the lantern ring. The upper part of the rotating paddle is provided with a sleeve with an opening, the lower part of the rotating paddle is provided with a blade and a magnetic bar, and the sleeve is inserted between the lantern ring and the upper baffle ring. By designing the connection mode of the fixed shaft and the rotating paddle, the number of accessories can be reduced, the fixed shaft and the rotating paddle can be fixedly connected only through a connector, and the installation steps of the magnetic stirring rod are simplified.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic stirring cell culture, and particularly relates to a magnetic stirring rod and a stirring type suspension cell culture bottle. Background Art

[0002] A magnetic stirring cell culture bottle is a culture container used in conjunction with a low-speed magnetic stirrer for cell culture. The principle is to use a magnet located at the bottom of the bottle to drive a suspension impeller or paddle to disturb the culture solution in the bottle, so that the cells remain in a suspended state. The magnetic stirring cell culture bottle is suitable for large-scale in vitro cell culture. For cell lines that can grow in suspension, using a suspension culture bottle can simply and economically culture a large number of cells. Using such a suspension culture bottle, the density of many suspension-adapted cell lines can reach 1x10 6 -2x10 6 cells per milliliter or even higher. Under the condition of culturing the same number of cells, the magnetic stirring cell culture bottle greatly reduces the workload required compared to using culture dishes, culture bottles, roller bottles or multi-layer culture devices.

[0003] Patent document CN215103320U discloses a novel stirring type cell culture bottle, which includes a bottle body, an upper cover and a stirring assembly. The upper cover is buckled on the top of the bottle body; the stirring assembly is located inside the bottle body and includes a mounting rod and a stirring paddle. The stirring paddle includes an outer cylinder, two blades oppositely arranged on the side wall of the outer cylinder, two mounting seats oppositely arranged on the side wall of the outer cylinder, an outer sleeve fixed on the mounting seat, and a magnet located inside the outer sleeve. The two mounting seats and the two blades are evenly distributed in the circumferential direction of the outer cylinder; the upper end of the mounting rod is arranged inside the upper cover. However, the above design of the magnetic stirring cell culture bottle has the defects of many components and complicated installation. Summary of the Utility Model

[0004] Therefore, the technical problem to be solved by the utility model is that the existing design of the magnetic stirring cell culture bottle has the defects of many components and complicated installation, so as to provide a magnetic stirring rod and a stirring type suspension cell culture bottle, the magnetic stirring rod has a small number of components and simplifies the installation steps of the magnetic stirring rod.

[0005] For this purpose, the utility model provides the following technical solutions:

[0006] A magnetic stirring rod, comprising:

[0007] A fixed shaft, the bottom of the fixed shaft is provided with a lower limit ring;

[0008] A connector, including a concentrically arranged collar and an upper retaining ring. The upper retaining ring is a retaining ring arranged on the upper part of the outer wall of the collar; the outer diameter of the lower limit ring of the fixed shaft is larger than the inner diameter of the collar; the collar is sleeved on the outer periphery of the fixed shaft, and the lower limit ring of the fixed shaft is limited at the bottom end of the collar;

[0009] A rotating paddle, an open sleeve is provided on the upper part of the rotating paddle, and blades and magnetic rods are provided on the lower part. The sleeve is inserted between the collar and the upper retaining ring.

[0010] Optionally, the outer diameter of the sleeve is larger than the inner diameter of the upper retaining ring, so that an interference fit is formed when the sleeve is inserted between the collar and the upper retaining ring.

[0011] Optionally, the outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.1 - 0.3 mm; and / or, the outer diameter of the lower limiting ring of the fixed shaft exceeds the inner diameter of the collar by 0.4 - 0.8 mm.

[0012] Optionally, the outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.2 mm; and / or, the outer diameter of the lower limiting ring of the fixed shaft exceeds the inner diameter of the collar by 0.6 mm.

[0013] Optionally, both between the outer wall of the sleeve and the inner wall of the upper retaining ring, and between the inner wall of the sleeve and the outer wall of the collar are further fixed by UV curing.

[0014] Optionally, the fixed shaft is cross-shaped.

[0015] Optionally, a cavity is provided at the bottom of the blade, and a magnetic rod is accommodated in the cavity.

[0016] Optionally, the cavities are symmetrically arranged on both sides of the blade.

[0017] Optionally, one of the openings in the cavity is in a sealed state.

[0018] A stirring type suspension cell culture flask, comprising a flask body, a bottle cap and the magnetic stirring rod arranged inside the flask body; the top end of the fixed shaft of the magnetic stirring rod is fixedly connected below the bottle cap.

[0019] The technical solution of the present utility model has the following advantages:

[0020] 1. The present utility model provides a magnetic stirring rod, comprising: a fixed shaft, a lower limiting ring is provided at the bottom of the fixed shaft; a connector, comprising a concentrically arranged collar and an upper retaining ring, the upper retaining ring is a retaining ring arranged on the upper part of the outer wall of the collar; the outer diameter of the lower limiting ring of the fixed shaft is larger than the inner diameter of the collar; the collar is sleeved on the outer periphery of the fixed shaft, and the lower limiting ring of the fixed shaft is limited at the bottom end of the collar; a rotating paddle, an open sleeve is provided on the upper part of the rotating paddle, and blades and magnetic rods are provided on the lower part. The sleeve is inserted between the collar and the upper retaining ring. By designing the connection method between the fixed shaft and the rotating paddle, the number of parts can be reduced, and only the connector is needed to fixedly connect the fixed shaft and the rotating paddle, simplifying the installation steps of the magnetic stirring rod.

[0021] 2. The present utility model provides a magnetic stirring rod. The outer diameter of the sleeve is larger than the inner diameter of the upper retaining ring, such that the sleeve is inserted between the collar and the upper retaining ring to form an interference fit. Through the interference fit, the rotating paddle can be more firmly connected to the outside of the sleeve.

[0022] 3. The present utility model provides a magnetic stirring rod. The outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.1 - 0.3 mm. Preferably, the outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.2 mm. It has been found that within the above range, a better interference fit is formed between the sleeve and the upper retaining ring. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific embodiments of the present utility model or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0024] Figure 1 is a schematic cross-sectional structure view of the magnetic stirring rod according to Embodiment 1 of the present utility model;

[0025] Figure 2 is a front view of the magnetic stirring rod according to Embodiment 1 of the present utility model;

[0026] Figure 3 is a side view of the magnetic stirring rod according to Embodiment 1 of the present utility model;

[0027] Figure 4 is a three-dimensional view of the magnetic stirring rod according to Embodiment 1 of the present utility model;

[0028] Figure 5 is a schematic connection view of the magnetic stirring rod and the bottle cap according to Embodiment 2 of the present utility model;

[0029] Figure 6 is a schematic view of the stirred suspension cell culture bottle according to Embodiment 3 of the present utility model.

[0030] 1 - fixed shaft, 11 - lower limit ring, 2 - connector, 21 - collar, 22 - upper retaining ring, 3 - rotating paddle, 31 - sleeve, 32 - blade, 321 - cavity, 33 - magnetic rod, 4 - bottle cap, 5 - bottle body. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] The following embodiments are provided to better understand the present utility model further, and are not limited to the best implementation mode. They do not constitute a limitation to the content and protection scope of the present utility model. Any product identical or similar to the present utility model obtained by anyone under the inspiration of the present utility model or by combining the features of the present utility model with those of other prior arts falls within the protection scope of the present utility model.

[0032] For those not specifying specific experimental steps or conditions in the embodiments, operations or conditions of conventional experimental steps described in the literature in this field can be followed. For reagents or instruments without indicating the manufacturer, they are all conventional reagent products that can be obtained through commercial purchase.

[0033] Embodiment 1

[0034] This embodiment provides a magnetic stirring rod, as Figures 1 - 4 shown, including:

[0035] A fixed shaft 1, with a lower limit ring 11 provided at the bottom of the fixed shaft 1. The lower limit ring 11 is a flange or round table surface extending outward from the bottom of the fixed shaft 1 for limiting.

[0036] A connector 2, including a concentrically arranged collar 21 and an upper retaining ring 22. The upper retaining ring 22 is a retaining ring provided on the upper part of the outer wall of the collar 21. The outer diameter of the lower limit ring 11 of the fixed shaft 1 is greater than the inner diameter of the collar 21. The collar 21 is sleeved on the outer periphery of the fixed shaft 1, and the lower limit ring 11 of the fixed shaft 1 is limited at the bottom end of the collar 21, so as to realize the connection between the fixed shaft 1 and the connector 2.

[0037] A rotating paddle 3, with an open sleeve 31 provided at the upper part, and blades 32 and a magnetic bar 33 provided at the lower part. The sleeve 31 is inserted between the collar 21 and the upper retaining ring 22, so as to realize the fixed connection between the rotating paddle 3 and the connector 2, and further realize the fixed connection between the fixed shaft 1 and the rotating paddle 3 through the connector 2, thus obtaining the magnetic stirring rod.

[0038] As a preferred implementation mode, the outer diameter of the sleeve 31 is greater than the inner diameter of the upper retaining ring 22, so that the sleeve 31 is inserted between the collar 21 and the upper retaining ring 22 to form an interference fit. Through the interference fit, the sleeve 31 of the rotating paddle 3 can be more firmly connected to the connector 2, and further the rotating paddle 3 can be more firmly connected to the connector 2. As a more preferred implementation mode, the outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.1 - 0.3 mm. Further more preferably, the outer diameter of the sleeve is selected to exceed the inner diameter of the upper retaining ring by 0.2 mm. As an optional implementation mode, the inner wall of the sleeve 31 and the outer wall of the upper retaining ring 22, and the outer wall of the sleeve 31 and the inner wall of the collar 21 can be further fixed by UV curing.

[0039] As a preferred embodiment, the outer diameter of the lower limit ring of the fixed shaft exceeds the inner diameter of the collar by 0.4 to 0.8 mm. Further more preferably, the outer diameter of the lower limit ring 11 of the fixed shaft 1 exceeds the inner diameter of the collar 21 by 0.6 mm. Within this range, it can better abut against the bottom of the collar 21, making it easier to position, so that the fixed connection between the fixed shaft 1 and the rotating paddle 3 through the connector 2 is less likely to become loose.

[0040] As a preferred embodiment, the fixed shaft 1 is cross-shaped, which reduces the weight of the fixed shaft on the premise of ensuring strength.

[0041] As an implementable way, a cavity 321 is provided at the bottom of the blade 32, and a magnetic rod 33 is accommodated in the cavity 321. As a preferred embodiment, the cavity 321 is symmetrically arranged on both sides of the blade 32. Such a symmetrical design makes it easier to control the accuracy of the mold during the injection molding process. As a preferred embodiment, one of the openings in the cavity is designed to be in a sealed state. When injecting the rotating paddle, directly inject one end opening into a sealed end. After putting the magnetic rod in, only the other end opening needs to be sealed. The process is simpler. The other opening can be sealed by using a sealing plug through ultrasonic welding or gluing.

[0042] As an implementable way, during assembly, the fixed shaft 1 is inserted into the collar 21 from the bottom of the connector 2, and then the sleeve 31 of the rotating paddle 3 is forcefully inserted between the collar 21 and the upper retaining ring 22 until it is completely fixed, forming an interference fit to achieve the assembly of the magnetic stirring rod. The method is simple and easy to operate.

[0043] As a preferred embodiment, the fixed shaft 1 and the connector 2 can be integrally formed by injection molding. The part of the rotating paddle 3 except for the sealing plug and the magnetic rod is also integrally formed. The magnetic rod is placed in the cavity, and then the sealing plug is fixed and sealed.

[0044] Embodiment 2

[0045] This embodiment provides a stirred suspension cell culture flask, as Figures 5 - 6 shown, including a flask body, a bottle cap and the magnetic stirring rod described in Embodiment 1 arranged inside the flask body; the top end of the fixed shaft 1 of the magnetic stirring rod is fixedly connected below the bottle cap.

[0046] Obviously, the above embodiments are only examples clearly described and not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A magnetic stirring rod, characterized in that, Comprising: A fixed shaft, at the bottom of which there is a lower limit ring; A connector, including a collar and an upper retaining ring arranged concentrically, the upper retaining ring being a retaining ring provided on the upper part of the outer wall of the collar; the outer diameter of the lower limit ring of the fixed shaft is larger than the inner diameter of the collar; the collar is sleeved on the outer periphery of the fixed shaft, and the lower limit ring of the fixed shaft is limited at the bottom end of the collar; A rotating paddle, on the upper part of which there is a sleeve with an opening, and on the lower part there are blades and a magnetic bar, and the sleeve is inserted between the collar and the upper retaining ring.

2. The magnetic stirring rod according to claim 1, wherein, The outer diameter of the sleeve is larger than the inner diameter of the upper retaining ring, so that an interference fit is formed when the sleeve is inserted between the collar and the upper retaining ring.

3. The magnetic stirring rod according to claim 2, characterized in that, The outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.1 - 0.3 mm; And / or, the outer diameter of the lower limit ring of the fixed shaft exceeds the inner diameter of the collar by 0.4 - 0.8 mm.

4. A magnetic stirring rod according to claim 3, characterized in that, The outer diameter of the sleeve exceeds the inner diameter of the upper retaining ring by 0.2 mm; And / or, the outer diameter of the lower limit ring of the fixed shaft exceeds the inner diameter of the collar by 0.6 mm.

5. A magnetic stirring rod according to claim 1, characterized in that, Both between the outer wall of the sleeve and the inner wall of the upper retaining ring, and between the inner wall of the sleeve and the outer wall of the collar are further fixed by UV curing.

6. A magnetic stirring rod according to any one of claims 1-5, characterized in that, The fixed shaft is cross-shaped.

7. A magnetic stirring rod according to any one of claims 1-5, characterized in that, At the bottom of the blade there is a cavity, and a magnetic bar is accommodated in the cavity.

8. A magnetic stirring rod according to claim 7, characterized in that, The cavities are symmetrically arranged on both sides of the blade.

9. A magnetic stirring rod according to claim 7, characterized in that, One of the openings in the cavity is in a sealed state.

10. A stirred suspension cell culture flask, characterized in that, Including a bottle body, a bottle cap, and the magnetic stirring rod according to any one of claims 1 - 9 arranged inside the bottle body; the top end of the fixed shaft of the magnetic stirring rod is fixedly connected below the bottle cap.

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