Cotton mature fiber paraffin embedding device

Abstract

The utility model discloses a cotton mature fiber embedding device, and relates to the technical field of fiber paraffin section manufacturing. The device comprises an embedding box (1), a first dense fiber comb (2) and a second dense fiber comb (3), the embedding box (1) is a cuboid groove-shaped device, and clamping grooves for fixing the first dense fiber comb (2) and the second dense fiber comb (3) are formed in two opposite side surfaces of the embedding box (1); the first dense fiber comb (2) or the second dense fiber comb (3) is made of stainless steel or high-density plastic, and the tail ends of comb teeth are sharp and can be repeatedly used; the depth of comb teeth of the first dense fiber comb (2) or the second dense fiber comb (3) is gradually deepened or deepened / shallower in a W shape from one end to the other end. The cotton mature fiber paraffin embedding device disclosed by the utility model is simple in structure, high in practicability and capable of effectively improving the paraffin slicing efficiency of the cotton mature fiber and ensuring the paraffin slicing quality.

Description

Technical Field

[0001] The utility model relates to the technical field of making paraffin sections of fibers, and particularly relates to a paraffin embedding device for cotton mature fibers. Background Technique

[0002] Paraffin sections are mainly used to observe the morphological structure inside tissues, for observing, differentiating and studying the morphological changes inside tissues, so as to better study the development and growth changes of sample materials. Excellent paraffin sections require characteristics such as complete sample structure, flat cut surface and appropriate thickness, and can clearly observe the tissue structure of the sample cut surface. Although the current paraffin section technology is already very mature and there are many paraffin embedding devices. However, there is currently no device specifically for embedding cotton mature fibers. Cotton fibers are very slender and soft tissues. When making sections, whether excellent paraffin sections can be made mainly depends on whether the tissues can be embedded in paraffin neatly and uniformly. The effect of paraffin embedding will directly affect the quality of paraffin sections, and also has a great impact on the observed tissue morphology, scoring, positional relationship, etc.

[0003] There are already many mature products for paraffin embedding devices for tissues that are easy to pick up and have a certain hardness. When using a conventional embedding device to make paraffin sections of cotton mature fibers, the fibers are easy to bend and not neat, resulting in uneven sections, thereby reducing the quality of cotton mature fiber sections and affecting the observation effect. Content of the Utility Model

[0004] In order to overcome the defect that the fibers of paraffin sections of cotton mature fibers are easy to bend and not neat described in the background technique, the utility model provides a paraffin embedding device for cotton mature fibers.

[0005] One object of the utility model is to provide a device suitable for paraffin embedding and wax infiltration of cotton mature fibers, which can quickly and simply make sections that can be used to observe the state of cotton mature fibers from soft and slender cotton mature fibers by using paraffin section technology;

[0006] Another object of the utility model is to provide a fiber comb suitable for fixing cotton mature fibers when making paraffin sections of cotton mature fibers, which can disperse cotton mature fibers evenly in paraffin and keep them in a straightened state.

[0007] In order to achieve the above objects, the utility model proposes the following technical solutions:

[0008] A paraffin embedding device for cotton mature fibers, comprising an embedding box (1), a first fiber comb (2), and a second fiber comb (3).

[0009] The embedding box (1) is a cuboid trough-shaped device, and there are card slots for fixing the first fiber comb (2) and the second fiber comb (3) on two opposite side surfaces;

[0010] The embedding cassette (1) is made of one or more of paper, medium-density polyethylene, and high-density polyethylene, is easy to cut, does not leak the embedding agent, and is not easily corroded by organic compounds.

[0011] The first fiber fine comb (2) or the second fiber fine comb (3) is made of one of stainless steel and high-density plastic, is not easily corroded by organic compounds, and the ends of the comb teeth are pointed.

[0012] The depth of the comb teeth of the first fiber fine comb (2) or the second fiber fine comb (3) gradually deepens or presents a "W" shape of deepening / shallowing from one end to the other end;

[0013] The first fiber fine comb (2) or the second fiber fine comb (3) has the same comb teeth, and the comb teeth are dense and of the same depth;

[0014] Further, except for the outermost comb teeth, the depth of the deepest comb teeth of the first fiber fine comb (2) or the second fiber fine comb (3) is not higher than the depth of the embedding cassette (1).

[0015] The width of the first fiber fine comb (2) or the second fiber fine comb (3) is slightly larger than the width of the embedding cassette (1);

[0016] The first fiber fine comb (2) or the second fiber fine comb (3) can slide on the opposite two side surfaces of the embedding cassette (1);

[0017] Further, except for the outermost comb teeth, the distance between any two adjacent comb teeth of the first fiber fine comb (2) or the second fiber fine comb (3) is 1-2 mm.

[0018] Further, the distance between the outermost comb teeth of any one of the pair of fiber fine combs is slightly larger than the thickness of the opposite two side surfaces of the embedding cassette (1), and the depth is higher than the depth of the embedding cassette (1), so as to be stuck on the opposite two side surfaces of the embedding cassette and can slide back and forth.

[0019] A method for using a paraffin embedding device for mature cotton fibers includes the following steps:

[0020] 1) Comb a bunch of mature cotton fibers evenly between the comb teeth of the first fiber fine comb (2) and the second fiber fine comb (3);

[0021] 2) Insert the first fiber fine comb (2) and the second fiber fine comb (3) with combed mature cotton on the opposite two side surfaces of the embedding cassette (1);

[0022] 3) Fix the first fiber fine comb (2) on the card slots at one end of the opposite two side surfaces of the embedding cassette (1);

[0023] 4) Slide the second fiber fine comb (3) slowly towards the other end on the opposite two sides of the embedding cassette (1), and snap it into the card slots at the other ends of the opposite two sides of the embedding cassette (1);

[0024] 5) Place the paraffin embedding device for cotton mature fibers in a high-temperature environment, and add liquid paraffin embedding agent to the embedding cassette (1);

[0025] 6) After the paraffin embedding agent completely submerges the cotton mature fibers, take out the paraffin embedding device for cotton mature fibers from the high-temperature environment after placing it for more than 12 hours;

[0026] 7) After the paraffin embedding agent cools, perform manual sectioning with a blade or mechanical sectioning with a microtome;

[0027] 8) The obtained fiber paraffin sections can be preserved or observed microscopically.

[0028] The above high-temperature environment is an environment higher than the melting point of the paraffin embedding agent by more than 3°C.

[0029] The beneficial effects of the present utility model are as follows: 1) After the cotton mature fibers are combed into the first fiber fine comb (2) and the second fiber fine comb (3), they can be relatively evenly distributed in the embedding agent according to a certain rule, which is conducive to the blade cutting the soft fibers; 2) The first fiber fine comb (2) or the second fiber fine comb (3) made of stainless steel can smoothly comb the cotton mature fibers and is not easily corroded by organic compounds; 3) The paraffin embedding device for cotton mature fibers has a simple structure and strong practicability. The fiber fine comb can be reused, is easy to process, has a low manufacturing cost, and is convenient for large-scale production and application; 4) When using the paraffin embedding device for cotton mature fibers to perform paraffin embedding on cotton mature fibers, the operation is simple and convenient. The cotton mature fibers can be neatly embedded in the paraffin, and it is convenient to position the cotton mature fibers in the paraffin, improving the sectioning efficiency, ensuring the flatness of the fiber sections, and having a high section quality. Description of the Drawings

[0030] Figure 1 It is a schematic diagram of the paraffin embedding device for cotton mature fibers.

[0031] Figure 2 It is a schematic diagram of the embedding cassette

[0032] Figure 3 It is a schematic diagram of a fiber fine comb with the same comb tooth depth.

[0033] Figure 4 It is a schematic diagram of a fiber fine comb with the comb tooth depth gradually increasing from one end to the other end.

[0034] Figure 5 It is a schematic diagram of a fiber fine comb with the comb tooth depth showing a "W" shape of deepening or shallowing from one end to the other end.

[0035] Figure 6 Schematic diagram of a fiber dense comb with the depth of the comb teeth showing a continuous "W" - shaped deepening or shallowing from one end to the other

[0036] Figure 7 Schematic diagram of the teeth of a fiber dense comb

[0037] In the figure: 1 is an embedding box; 2 and 3 are a pair of fiber dense combs; 4 and 5 are card slots Detailed implementation mode

[0038] To make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the following further elaborates on the present invention in conjunction with specific embodiments

[0039] A cotton mature fiber embedding device includes an embedding box (1), a first fiber dense comb (2), and a second fiber dense comb (3), as Figure 1 shown

[0040] The embedding box (1) is a cuboid - shaped trough - type device, and there are card slots on two opposite side surfaces for fixing the first fiber dense comb (2) and the second fiber dense comb (3), as Figure 2 shown; the material of the embedding box (1) is one or several of paper, medium - density polyethylene, and high - density polyethylene, which is easy to cut, does not leak the embedding agent, and is not easily corroded by organic compounds

[0041] The material of the first fiber dense comb (2) or the second fiber dense comb (3) is stainless steel, which is not easily corroded by the organic compound xylene, and the ends of the comb teeth are pointed (as Figure 7 shown). The depth of the comb teeth of the first fiber dense comb (2) or the second fiber dense comb (3) gradually deepens or shows a "W" - shaped deepening / shallowing from one end to the other; the first fiber dense comb (2) or the second fiber dense comb (3) has the same comb teeth, and the comb teeth are close and have the same depth (as Figure 3 shown);

[0042] Furthermore, for the first fiber dense comb (2) or the second fiber dense comb (3), except for the outermost comb teeth, the depth of the deepest comb teeth is not higher than the depth of the embedding box (1); the width of the first fiber dense comb (2) or the second fiber dense comb (3) is slightly larger than the width of the embedding box (1); the first fiber dense comb (2) or the second fiber dense comb (3) can slide on two opposite side surfaces of the embedding box (1);

[0043] Furthermore, for the first fiber dense comb (2) or the second fiber dense comb (3), except for the outermost comb teeth, the distance between any two adjacent comb teeth is 1 - 2 mm

[0044] Further, the distance between the outermost comb teeth of any one of the pair of fiber combs is slightly larger than the thickness of the opposite two side faces of the embedding cassette (1), and the depth is higher than the depth of the embedding cassette (1), so as to be stuck on the opposite two side faces of the embedding cassette and can slide back and forth.

[0045] A method for using a paraffin embedding device for cotton mature fibers, comprising the following steps:

[0046] 1) Comb a bunch of cotton mature fibers evenly between the comb teeth of the first fiber comb (2) and the second fiber comb (3);

[0047] 2) Insert the first fiber comb (2) and the second fiber comb (3) with combed cotton maturity on the opposite two side faces of the embedding cassette (1);

[0048] 3) Fix the first fiber comb (2) on the card slots at one end of the opposite two side faces of the embedding cassette (1);

[0049] 4) Slowly slide the second fiber comb (3) on the opposite two side faces of the embedding cassette (1) towards the other end, and snap it into the card slots at the other end of the opposite two side faces of the embedding cassette (1);

[0050] 5) Place the paraffin embedding device for cotton mature fibers in a high-temperature environment above 3 °C higher than the melting point of the paraffin embedding agent, and add liquid paraffin embedding agent to the embedding cassette (1);

[0051] 6) After the paraffin embedding agent completely submerges the cotton mature fibers, leave it for more than 12 h and then take out the paraffin embedding device for cotton mature fibers from the high-temperature environment;

[0052] 7) After the paraffin embedding agent cools, manually slice with a blade or mechanically slice with a microtome;

[0053] 8) The obtained fiber paraffin sections can be preserved or microscopically observed.

[0054]

Example 1

[0055] A paraffin embedding device for cotton mature fibers, comprising an embedding cassette (1), a first fiber comb (2), and a second fiber comb (3).

[0056] The embedding cassette (1) is a rectangular parallelepiped trough-shaped device, and there are card slots for fixing the first fiber comb (2) and the second fiber comb (3) on the opposite two side faces. The width of the rectangular parallelepiped is 3.0 cm, the length is 5.0 cm, and the depth is 3.0 cm; the material of the embedding cassette (1) is paper, and the thickness of the opposite two side faces is 1.5 mm, which is easy to cut, does not leak the embedding agent, and is not easily corroded by organic compounds.

[0057] The material of the first fiber fine comb (2) or the second fiber fine comb (3) is high-density plastic and is not easily corroded by organic compounds. The depth of the teeth of the first fiber fine comb (2) or the second fiber fine comb (3) gradually increases (as shown in Figure 4 ); the first fiber fine comb (2) or the second fiber fine comb (3) has the same teeth, and the teeth are close and of the same depth;

[0058] Furthermore, except for the outermost teeth, the depth of the deepest teeth of the first fiber fine comb (2) or the second fiber fine comb (3) is not higher than the depth of the embedding cassette (1), which is 2.8 cm; the width of the first fiber fine comb (2) or the second fiber fine comb (3) is slightly larger than the width of the embedding cassette (1), which is 3.5 cm; the first fiber fine comb (2) or the second fiber fine comb (3) can slide on the opposite two side surfaces of the embedding cassette (1);

[0059] Furthermore, except for the outermost teeth, the distance between any two adjacent teeth of the first fiber fine comb (2) or the second fiber fine comb (3) is 1 mm.

[0060] Furthermore, the distance between the outermost teeth of any one of the pair of fiber fine combs is 1.6 mm, which is slightly larger than the thickness of the opposite two side surfaces of the embedding cassette (1), and the depth of the teeth is 3.2 cm, higher than the depth of the embedding cassette (1), so as to be stuck on the opposite two side surfaces of the embedding cassette and can slide back and forth.

[0061]

Example 2

[0062] A cotton mature fiber embedding device includes an embedding cassette (1), a first fiber fine comb (2), and a second fiber fine comb (3).

[0063] The embedding cassette (1) is a cuboid trough-shaped device, and there are slots for fixing the first fiber fine comb (2) and the second fiber fine comb (3) on the opposite two side surfaces. The width of the cuboid is 3.0 cm, the length is 3.0 cm, and the depth is 3.0 cm; the material of the embedding cassette (1) is medium-density polyethylene, and the thickness of the opposite two side surfaces is 2.0 mm, which is easy to cut, does not leak the embedding agent, and is not easily corroded by organic compounds.

[0064] The material of the first fiber fine comb (2) or the second fiber fine comb (3) is stainless steel and is not easily corroded by organic compounds. The ends of the teeth of the first fiber fine comb (2) or the second fiber fine comb (3) are pointed. The depth of the teeth of the first fiber fine comb (2) or the second fiber fine comb (3) shows a "W" shape of deepening or shallowing from one end to the other end (as shown in Figure 5 ); the first fiber fine comb (2) or the second fiber fine comb (3) has the same teeth, and the teeth are close and of the same depth;

[0065] Further, except for the outermost comb teeth, the deepest comb tooth depth of the first fiber comb (2) or the second fiber comb (3) is not higher than the depth of the embedding cassette (1), which is 2.8 cm; the width of the first fiber comb (2) or the second fiber comb (3) is slightly larger than the width of the embedding cassette (1), which is 3.5 cm; the first fiber comb (2) or the second fiber comb (3) can slide on the opposite two side surfaces of the embedding cassette (1);

[0066] Further, except for the outermost comb teeth, the distance between any two adjacent comb teeth in the first fiber comb (2) or the second fiber comb (3) is 1.5 mm.

[0067] Further, the distance between any one of the outermost comb teeth in the pair of fiber combs is 2.2 mm, which is slightly larger than the thickness of the opposite two side surfaces of the embedding cassette (1), and the comb tooth depth is 3.3 cm higher than the depth of the embedding cassette (1), so as to be stuck on the opposite two side surfaces of the embedding cassette and can slide back and forth.

[0068]

Example 3

[0069] A cotton mature fiber embedding device includes an embedding cassette (1), a first fiber comb (2), and a second fiber comb (3).

[0070] The embedding cassette (1) is a cuboid trough-shaped device, and there are card slots for fixing the first fiber comb (2) and the second fiber comb (3) on the opposite two side surfaces. The cuboid has a width of 4.0 cm, a length of 8.0 cm, and a depth of 3.5 cm; the material of the embedding cassette (1) is high-density polyethylene, and the thickness of the opposite two side surfaces is 1.0 mm, which is easy to cut, does not leak the embedding agent, and is not easily corroded by organic compounds.

[0071] The material of the first fiber comb (2) or the second fiber comb (3) is stainless steel, which is not easily corroded by organic compounds, and the ends of the comb teeth are pointed. The comb tooth depth of the first fiber comb (2) or the second fiber comb (3) shows continuous "W" - shaped deepening or shallowing from one end to the other end (as Figure 6 shown); the first fiber comb (2) or the second fiber comb (3) has the same comb teeth, and the comb teeth are close and have the same depth;

[0072] Further, except for the outermost comb teeth, the deepest comb tooth depth of the first fiber comb (2) or the second fiber comb (3) is not higher than the depth of the embedding cassette (1), which is 3.0 cm; the width of the first fiber comb (2) or the second fiber comb (3) is slightly larger than the width of the embedding cassette (1), which is 4.5 cm; the first fiber comb (2) or the second fiber comb (3) can slide on the opposite two side surfaces of the embedding cassette (1);

[0073] Further, except for the outermost comb teeth, the distance between any two adjacent comb teeth in the first fiber densifier (2) or the second fiber densifier (3) is 2.0 mm.

[0074] Further, the distance between any one of the outermost comb teeth in a pair of fiber densifiers is 2.2 mm, slightly greater than the thickness of the opposite two sides of the embedding cassette (1), and the depth of the comb teeth is 3.8 cm, higher than the depth of the embedding cassette (1), so as to be stuck on the opposite two sides of the embedding cassette and can slide back and forth.

[0075]

Example 4

[0076] A method for using the paraffin embedding device for cotton mature fibers in Example 1 comprises the following steps:

[0077] 1) Comb a bunch of cotton mature fibers evenly between the comb teeth of the first fiber densifier (2) and the second fiber densifier (3);

[0078] 2) Insert the first fiber densifier (2) and the second fiber densifier (3) with combed cotton maturity on the opposite two sides of the embedding cassette (1);

[0079] 3) Fix the first fiber densifier (2) on the card slots at one end of the opposite two sides of the embedding cassette (1);

[0080] 4) Slowly slide the second fiber densifier (3) on the opposite two sides of the embedding cassette (1) towards the other end, so that the fibers between the comb teeth are in a straightened state, and then insert it into the card slots at the other end of the opposite two sides of the embedding cassette (1);

[0081] 5) Put the paraffin embedding device for cotton mature fibers into a high-temperature environment of 60 °C, and add liquid paraffin embedding agent (melting point is 56 °C) into the embedding cassette (1);

[0082] 6) After the paraffin embedding agent completely submerges the cotton mature fibers, take out the paraffin embedding device for cotton mature fibers from the high-temperature environment after standing for 12 h;

[0083] 7) After the paraffin embedding agent cools, manually slice with a blade or mechanically slice with a slicing machine;

[0084] 8) The obtained fiber paraffin sections can be observed microscopically.

[0085]

Comparative Example 1

[0086] Wind the fibers into bundles with fine threads and fix them overnight at room temperature with FAA fixative. Discard the waste liquid, add a series of dehydration solutions to the samples, dehydrate them step by step to the VI-level dehydration solution (stay for 2 h at each level), and dehydrate twice at the VI level. Add fresh tert-butanol and an equal volume of paraffin, and incubate at 65 °C for 2 - 3 h. Replace with fresh paraffin completely and incubate overnight at 65 °C. Add melted paraffin to the folded small paper box, quickly put in the fiber samples, straighten them with forceps, lift appropriately, and place on a condenser to solidify. After solidification, it can be used for sectioning.

[0087] After trimming the embedded materials, use a manual rotary microtome (Leica, RM2235) to section. The section thickness is 10 - 20 μm. Stick the wax ribbons onto glass slides with Haupt adhesive and let them dry. Place the dried sections in a xylene substitute until the paraffin dissolves. Rehydrate the sections step by step in xylene substitute:ethanol (1:1), 100% ethanol, 95% ethanol, 85% ethanol, and 70% ethanol, staying for 5 min at each level. Transfer to safranin alcohol stain and stain for 1 h. Dehydrate the sections step by step in 70% ethanol, 85% ethanol, fast green alcohol stain for 30 s, 95% ethanol, 100% ethanol, xylene substitute:ethanol (1:1), and xylene substitute, staying for 5 min at each level. Take out the sections, drop Canada balsam, cover with cover slips, and let them dry. Take pictures of the sections under a stereomicroscope (Olympus, SZX9).

[0088]

Comparative Example 2

[0089] 1) Fixation: After carding the mature fibers, take a small amount of fibers from the middle of cotton seeds, cross-tie them into bundles with fine linen threads, and fix them overnight with FAA fixative (5 mL 38% formaldehyde: 5 mL glacial acetic acid: 90 mL 70% alcohol).

[0090] 2) Dehydration: Dehydrate step by step with 95% (V / V) and 100% (V / V) alcohol for 2 - 3 h; dehydrate twice with absolute ethanol:tert-butanol (1:1), 2 - 3 h for the first time and overnight for the second time; infiltrate with tert-butanol 3 times, 2 - 3 h for the first two times and overnight for the last time.

[0091] 3) Wax infiltration: Infiltrate with tert-butanol:paraffin (1:1) 3 times, 2 - 3 h for the first two times and overnight for the last time; infiltrate with paraffin 3 times, 2 - 3 h for the first two times and overnight for the last time; the whole process is carried out in an incubator at 65 °C.

[0092] 4) Embedding: Put the fibers into an embedding mold containing resin, and adjust the fiber positions with forceps; pull both sides of the embedding mold with both hands until the resin is completely solidified.

[0093] 5) Sectioning: Take out the resin block from the embedding cassette. Select the end farther from the material as the bottom, trim it flat with a blade and fix it on the microtome (Leica, RM2235). Set the cross-section thickness to 10 μm. Add a drop of clear water onto a glass slide coated with Haup adhesive, slowly shake to spread the clear water evenly over the glass slide, and then place the section on the glass slide.

[0094] 6) Photographing: Observe and take pictures with an upright microscope.

[0095] In summary, a cotton mature fiber embedding device proposed by the present utility model solves the problems of the existing paraffin embedding device, and can effectively solve the technical problems that the paraffin sections of cotton mature fibers are prone to bending and being uneven.

[0096] Those of ordinary skill in the art should understand that the above are only specific embodiments of the present utility model and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A paraffin embedding device for mature cotton fibers, characterized in that It includes an embedding cassette (1), a first fiber fine comb (2), and a second fiber fine comb (3); the embedding cassette (1) is a cuboid trough-shaped device, and there are clamping grooves for fixing the first fiber fine comb (2) and the second fiber fine comb (3) on two opposite side faces; the comb tooth depth of the first fiber fine comb (2) or the second fiber fine comb (3) gradually deepens or presents a "W" shape of deepening / shallowing from one end to the other end; the first fiber fine comb (2) or the second fiber fine comb (3) has the same comb teeth, and the comb teeth are close and of the same depth.

2. The paraffin embedding device for mature cotton fibers according to claim 1, wherein The material of the embedding cassette (1) is one or several of paper, medium-density polyethylene, and high-density polyethylene; the material of the first fiber fine comb (2) or the second fiber fine comb (3) is one of stainless steel and high-density plastic, and the ends of the comb teeth are pointed.

3. The paraffin embedding device for mature cotton fibers according to claim 1, characterized in that, Except for the outermost comb teeth, the depth of the deepest comb teeth of the first fiber fine comb (2) or the second fiber fine comb (3) is not higher than the depth of the embedding cassette (1); the width of the first fiber fine comb (2) or the second fiber fine comb (3) is slightly larger than the width of the embedding cassette (1).

4. A paraffin embedding device for mature cotton fibers according to claim 1, characterized in that, Except for the outermost comb teeth, the distance between any two adjacent comb teeth in the first fiber fine comb (2) or the second fiber fine comb (3) is 1-2 mm; further, the distance between any one of the outermost comb teeth in a pair of fiber fine combs is slightly larger than the thickness of the two opposite side faces of the embedding cassette (1), and the depth is higher than the depth of the embedding cassette (1).

Images