Handheld fluorescence immunoassay analyzer

Abstract

The utility model provides a handheld fluorescence immunoassay analyzer which comprises a bottom shell and an upper shell, the bottom shell and the upper shell are connected to form a containing space, and a main control board, a mounting substrate, a battery, a battery pressing piece, a reagent card bin assembly and a fluorescence light box assembly are arranged in the containing space; a battery is arranged above the bottom shell, a battery pressing piece is arranged above the battery, the battery pressing piece is fixedly connected with the bottom shell, and the battery is fixed on the bottom plate; a main control board is arranged above the battery pressing piece, a mounting substrate is arranged above the main control board, and a reagent card bin assembly and a fluorescent light box assembly are mounted on the mounting substrate. The handheld fluorescence immunoassay analyzer disclosed by the utility model solves the problems that the existing equipment is limited in use scene, inconvenient to carry about, free of a temperature incubation function and unstable in detection sensitivity, and has the advantages of high integration, miniaturization, heating incubation, adaptation to a low-temperature environment and stable detection sensitivity.

Description

Technical Field

[0001] The utility model relates to the field of fluorescence detection, and particularly to a handheld fluorescence immunoassay analyzer. Background Art

[0002] Fluorescence immunoassay chromatography technology is a new membrane detection technology based on specific antigen-antibody immune reactions. This method has the characteristics of specificity, simple operation, rapidity, etc., and is widely used in important fields such as clinical diagnosis, environmental monitoring, food safety, etc. At present, there are various types of fluorescence immunoassay analyzers on the market, which can be classified into single-channel fluorescence immunoassay analyzers, multi-channel fluorescence immunoassay analyzers, and fully automatic fluorescence immunoassay analyzers according to categories. Different types of instruments can be selected according to application scenarios. However, there are not many handheld portable fluorescence immunoassay analyzers. Handheld instruments can be carried around, with flexible usage scenarios, bringing great convenience to users and better serving patients.

[0003] Most of the fluorescence immunoassay analyzers on the market are desktop types, including single-channel, multi-channel, and fully automatic forms. However, the above products have limited application scenarios, are inconvenient to carry around, and basically do not have a temperature incubation function for handheld instruments on the market. They cannot provide a suitable reaction environment for sample reactions in a low-temperature environment, seriously affecting the detection sensitivity and resulting in misinterpretation of detection results. Summary of the Utility Model

[0004] The main purpose of the utility model is to propose a handheld fluorescence immunoassay analyzer, which overcomes the above technical problems.

[0005] To achieve the above purpose, the utility model proposes the following technical solutions:

[0006] A handheld fluorescence immunoassay analyzer, comprising:

[0007] A bottom case and an upper case, the bottom case and the upper case are connected to form an accommodation space, and the following are arranged in the accommodation space: a main control board, a mounting substrate, a battery, a battery pressing part, a reagent card bin assembly, and a fluorescence light box assembly;

[0008] A battery is arranged above the bottom case, a battery pressing part is arranged above the battery, and the battery pressing part is fixedly connected to the bottom case to fix the battery on the bottom case;

[0009] A main control board is arranged above the battery pressing part, a mounting substrate is arranged above the main control board, and a reagent card bin assembly and a fluorescence light box assembly are mounted on the mounting substrate;

[0010] The reagent card cartridge assembly includes: a lead screw stepper motor, a motor base, a reagent card cartridge, a heating film, a heat - uniforming plate, a connecting member, and a linear guide. The lead screw stepper motor is fixedly connected to the mounting substrate through the motor base. The reagent card cartridge is connected to the lead screw of the lead screw stepper motor. The reagent card cartridge is also slidably connected to the linear guide through the connecting member. A heating film is provided on the upper surface of the bottom of the reagent card cartridge, and the heat - uniforming plate is adhered to the upper surface of the heating film.

[0011] Further, the fluorescence light box assembly includes: a fluorescence light box, a scanning module, and a fixing bracket. The fluorescence light box is connected to the mounting substrate through the fixing bracket, and the scanning module is connected to the fixing bracket.

[0012] Further, an LED board is provided inside the fluorescence light box. On one side of the lower end of the LED board, a first filter and a first plano - convex lens are sequentially provided. A dichroic mirror is provided on the side of the first plano - convex lens away from the first filter. A cylindrical lens is provided below the dichroic mirror, a second plano - convex lens is provided above the dichroic mirror, a second filter is provided above the second plano - convex lens, and a receiving board is provided above the second filter.

[0013] Further, the reagent card cartridge assembly further includes a temperature probe and a temperature fuse. Both the temperature probe and the temperature fuse are connected to the heating film for temperature control and over - temperature protection. The reagent card cartridge is also provided with a through - slot for the heating film to lead out wires.

[0014] Further, a zero - position optoelectronic device is also provided on the mounting substrate, which cooperates with the lead screw stepper motor and the linear guide 17 to realize the detection of the high - precision and stable operation of the reagent card.

[0015] Further, the upper shell is also provided with a switch button for controlling the on - off of the overall circuit of the handheld fluorescence immunoassay analyzer.

[0016] Further, the reagent card cartridge is provided with a first protrusion and a second protrusion for fixing the reagent card.

[0017] Further, a display screen is provided above the upper shell.

[0018] Further, it is characterized in that the reagent card cartridge is also provided with a receiving groove for accommodating the temperature probe and the temperature fuse.

[0019] Further, a printer is installed on the upper shell.

[0020] Through the handheld fluorescence immunoassay analyzer of the present utility model, the problems of limited use scenarios of existing devices, inconvenience in carrying around, lack of temperature incubation function, and unstable detection sensitivity are solved. It has the advantages of high integration, miniaturization, heat - able incubation, adaptability to low - temperature environments, and stable detection sensitivity. Brief Description of the Drawings

[0021] The accompanying drawings forming a part of this application are used to provide a further understanding of the present utility model. The schematic embodiments of the present utility model and their descriptions are used to explain the present utility model and do not constitute an improper limitation to the present utility model. In the drawings:

[0022] Figure 1 It is a three-dimensional assembly diagram of a handheld fluorescence immunoassay analyzer of the present utility model.

[0023] Figure 2 It is a schematic structural diagram of a temperature probe and a temperature fuse.

[0024] Figure 3 and Figure 4 They are schematic diagrams of a reagent card bin at different angles.

[0025] Figure 5 It is a schematic diagram of the internal structure of a fluorescence light box.

[0026] Among them, the above-mentioned drawings include the following reference numerals:

[0027] 01, bottom case; 02, upper case; 03, display screen; 04, printer; 05, main control board; 06, mounting substrate; 07, battery; 08, battery pressing part; 09, lead screw stepping motor; 10, motor base; 11, reagent card bin; 12, heating film; 13, heat equalizing plate; 14, temperature probe; 15, temperature fuse; 16, connecting piece; 17, linear track; 18, zero position optoelectronic; 19, switch button; 20, fluorescence light box; 21, scanning module; 22, fixing bracket; 110, first protrusion; 111, through slot; 112, second protrusion; 113, receiving groove; 201, LED board; 202, receiving board; 203, first filter; 204, first plano-convex lens; 205, dichroic mirror; 206, cylindrical lens; 207, second plano-convex lens; 208, second filter. Detailed implementation manners

[0028] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. The description of at least one exemplary embodiment below is actually only illustrative and in no way limits the present utility model and its application or use. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts belong to the scope of protection of the present utility model.

[0029] It should be noted that the terms used herein are for the purpose of describing specific embodiments only and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular forms are also intended to include the plural forms. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they specify the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0030] Unless otherwise specifically stated, the relative arrangements of the components and steps set forth in these embodiments, numerical expressions, and values do not limit the scope of the present invention. At the same time, it should be understood that, for the sake of convenience of description, the dimensions of the various parts shown in the drawings are not drawn in actual proportional relationships. Technologies, methods, and devices known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the said technologies, methods, and devices should be regarded as part of the authorized specification. In all the examples shown and discussed here, any specific value should be construed as merely exemplary and not as a limitation. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that like reference numerals and letters denote like items in the following drawings, and thus, once an item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0031] Next, with reference to Figures 1 to 5 , the present invention will be further described:

[0032] A handheld fluorescence immunoassay analyzer, comprising:

[0033] A bottom shell 01 and a top shell 02, the bottom shell 01 and the top shell 02 are connected to form an accommodation space, and the following are arranged in the accommodation space: a main control board 05, a mounting substrate 06, a battery 07, a battery pressing member 08, a reagent card bin assembly, and a fluorescence light box assembly;

[0034] A battery 07 is arranged above the bottom shell 01, a battery pressing member 08 is arranged above the battery 07, and the battery pressing member 08 is fixedly connected to the bottom shell 01 to fix the battery 07 on the bottom shell 01;

[0035] A main control board 05 is arranged above the battery pressing member 08, a mounting substrate 06 is arranged above the main control board, and a reagent card bin assembly and a fluorescence light box assembly are mounted on the mounting substrate 06;

[0036] The reagent card bin assembly includes: a lead screw stepper motor 09, a motor base 10, a reagent card bin 11, a heating film 12, a heat - uniforming plate 13, a connecting piece 16, and a linear guide 17. The lead screw stepper motor 09 is fixedly connected to the mounting substrate 06 through the motor base 10. The reagent card bin 11 is connected to the lead screw of the lead screw stepper motor 09. The reagent card bin 11 is also slidably connected to the linear guide 17 through the connecting piece 16. A heating film 12 is provided on the upper surface of the bottom of the reagent card bin 11, and the heat - uniforming plate 13 is pasted on the upper surface of the heating film 12.

[0037] The reagent card bin assembly provides a loading position for the reagent card and a suitable incubation temperature. The lead screw nut in the lead screw stepper motor 09 is fixedly connected to the side fixing holes of the reagent card bin. The reagent card bin is fixed to the linear guide 17 through the connecting piece 16. The lead screw stepper motor 09 cooperates with the linear guide 17 to drive the reagent card assembly to move smoothly and evenly, realizing the scanning detection of the detection line position of the reagent card and reading the fluorescence signal value.

[0038] In this embodiment, the reagent card bin 11 is provided with a first protrusion 110 and a second protrusion 112 for fixing the reagent card, and is also provided with a through - slot 111 for the heating film to lead out. The heating film lead - out interface is connected to the temperature control interface of the main control board to realize temperature control. The heating film 12 is pasted on the inner bottom of the reagent card bin, and the heat - uniforming plate 13 is pasted on the heating film 12.

[0039] In this embodiment, the reagent card bin assembly further includes a temperature probe 14 and a temperature fuse 15. The temperature probe 14 and the temperature fuse 15 are both connected to the heating film 12 for temperature control and over - temperature protection. The reagent card bin 11 is also provided with a through - slot 111 for the heating film to lead out.

[0040] The temperature probe 14 and the temperature fuse 15 are welded to the back pads of the heating film 12. The reagent card bin 11 has a corresponding receiving groove 113. After welding, thermal conductive glue is used for glue sealing to play a role in heat conduction and fixation.

[0041] In this embodiment, the fluorescence light box assembly includes: a fluorescence light box 20, a scanning module 21, and a fixing bracket 22. The fluorescence light box 20 is connected to the mounting substrate 06 through the fixing bracket 22, and the scanning module 21 is connected to the fixing bracket 22.

[0042] In this embodiment, an LED board 201 is disposed inside the fluorescent light box 20. On one side of the lower end of the LED board 201, a first filter 203 and a first plano-convex lens 204 are sequentially arranged. On the side of the first plano-convex lens 204 away from the first filter 203, a dichroic mirror 205 is provided. Below the dichroic mirror 205, a cylindrical lens 206 is provided. Above the dichroic mirror 205, a second plano-convex lens 207 is provided. Above the second plano-convex lens 207, a second filter 208 is provided. Above the second filter 208, a receiving board 202 is provided.

[0043] The LED board 201 is used to provide an excitation light source for the optical path, and the receiving board 202 is used to receive the excited fluorescence signal. The LED light source emits light and passes through the first filter 203. The first plano-convex lens 204 corrects the light into parallel light. Then, the dichroic mirror 205 reflects the parallel light downward and passes through the cylindrical lens 206. The cylindrical lens 206 converges the parallel light into a linear light spot or a rectangular light spot, and the thickness dimension of the light spot is adjustable according to the focal length of the lens. The excitation light spot irradiates the detection line on the reagent card, and the reaction substance on the detection line excites a fluorescence signal, which is converged into parallel light by the cylindrical lens 206, passes through the dichroic mirror 205, is converged by the second plano-convex lens 207, then passes through the second filter 208, and is received by the receiving board 202 on the receiving board. The receiver can be a photoelectric receiver such as a PD or a PMT.

[0044] Generally, for a circular light spot, the excitation efficiency is not high relative to the sample detection line, and the signal background noise is large. However, the cylindrical lens 206 in the optical system of this application converges the excitation light source into a linear light spot or a rectangular light spot, with high light efficiency, and can just cover the sample detection line, and the signal noise is low.

[0045] In this embodiment, a zero-position optoelectronic device 18 is further provided on the mounting substrate 06, which cooperates with the lead screw stepping motor 09 and the linear guide 17 to realize the high-precision and stable operation detection of the reagent card.

[0046] In this embodiment, the upper shell 02 is further provided with a switch button 19 for controlling the on / off of the overall circuit of the handheld fluorescence immunoassay analyzer.

[0047] In this embodiment, a display screen 03 is provided above the upper shell 02 for information display.

[0048] In this embodiment, it is characterized in that the reagent card bin 11 is further provided with a receiving groove 113 that can accommodate a temperature probe 14 and a temperature fuse 15.

[0049] In this embodiment, a printer 04 is installed on the upper shell 02, and a test report can be printed after the detection is completed.

[0050] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom", etc. is usually based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description. Without contrary explanations, these orientation words do not indicate and imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation on the protection scope of the present utility model; the orientation words "inside, outside" refer to the inside and outside relative to the contour of each component itself.

[0051] The above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. For those skilled in the art, the present utility model can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A handheld fluorescence immunoassay analyzer, characterized in that, Comprising: A bottom case (01) and an upper case (02), the bottom case (01) and the upper case (02) are connected to form an accommodation space, and the accommodation space is provided with: a main control board (05), a mounting substrate (06), a battery (07), a battery pressing member (08), a reagent card bin assembly, and a fluorescence light box assembly; The battery (07) is arranged above the bottom case (01), the battery pressing member (08) is arranged above the battery (07), and the battery pressing member (08) is fixedly connected to the bottom case (01) to fix the battery (07) on the bottom case (01); The main control board (05) is arranged above the battery pressing member (08), the mounting substrate is arranged above the main control board, and the reagent card bin assembly and the fluorescence light box assembly are mounted on the mounting substrate (06); The reagent card bin assembly includes: a lead screw stepping motor (09), a motor seat (10), a reagent card bin (11), a heating film (12), a heat - equalizing plate (13), a connecting member (16), and a linear guide (17). The lead screw stepping motor (09) is fixedly connected to the mounting substrate (06) through the motor seat (10). The reagent card bin (11) is connected to the lead screw of the lead screw stepping motor (09), and the reagent card bin (11) is also slidably connected to the linear guide (17) through the connecting member (16). A heating film (12) is arranged on the upper surface of the bottom of the reagent card bin (11), and the heat - equalizing plate (13) is pasted on the upper surface of the heating film (12).

2. The hand-held fluorescence immunoassay analyzer according to claim 1, wherein The fluorescence light box assembly includes: a fluorescence light box (20), a scanning module (21), and a fixing bracket (22). The fluorescence light box (20) is connected to the mounting substrate (06) through the fixing bracket (22), and the scanning module (21) is connected to the fixing bracket (22).

3. The handheld fluorescence immunoassay analyzer according to claim 2, wherein Inside the fluorescence light box (20), an LED board (201) is arranged. On one side of the lower end of the LED board (201), a first filter (203) and a first plano - convex lens (204) are arranged in sequence. A dichroic mirror (205) is arranged on the side of the first plano - convex lens (204) away from the first filter (203). A cylindrical lens (206) is arranged below the dichroic mirror (205). A second plano - convex lens (207) is arranged above the dichroic mirror (205). A second filter (208) is arranged above the second plano - convex lens (207). A receiving board (202) is arranged above the second filter (208).

4. A hand-held fluorescence immunoassay analyzer according to claim 1, wherein, The reagent card bin assembly further includes a temperature probe (14) and a temperature fuse (15). The temperature probe (14) and the temperature fuse (15) are both connected to the heating film (12) for temperature control and over - temperature protection. The reagent card bin (11) is also provided with a through - hole notch (111) for the heating film wire to pass through.

5. A handheld fluorescence immunoassay analyzer according to claim 1, wherein, A zero - position optoelectronic device (18) is also arranged on the mounting substrate (06), which cooperates with the lead screw stepping motor (09) and the linear guide (17) to realize the high - precision and stable operation detection of the reagent card.

6. The handheld fluorescence immunoassay analyzer according to claim 1, characterized in that, The upper shell (02) is also provided with a switch button (19) for controlling the on / off of the overall circuit of the handheld fluorescence immunoassay analyzer.

7. A handheld fluorescence immunoassay analyzer according to claim 1, characterized in that, The reagent card bin (11) is provided with a first protrusion (110) and a second protrusion (112) for fixing the reagent card.

8. The hand-held fluorescence immunoassay analyzer according to claim 1, characterized in that, A display screen (03) is arranged above the upper shell (02).

9. The hand-held fluorescence immunoassay analyzer according to claim 4, wherein The reagent card bin (11) is further provided with a receiving groove (113) for accommodating the temperature probe (14) and the temperature fuse (15).

10. A handheld fluorescence immunoassay analyzer according to claim 1, characterized in that, A printer (04) is installed on the upper shell (02).

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