Extraction and purification instrument for DNA / RNA (deoxyribonucleic acid / ribonucleic acid) nucleic acid

Abstract

The utility model discloses an extraction and purification instrument for DNA / RNA (deoxyribonucleic acid / ribonucleic acid) nucleic acid, and relates to the technical field of extraction and purification instruments for DNA / RNA nucleic acid, in particular to a base, a fixing frame is fixedly connected to the upper surface of the base, a sliding groove is formed in the front of the fixing frame, and a rotating disc is fixedly connected to one end of an output shaft of a motor. A connecting rod is rotatably connected to the inner side wall of the test tube fixing frame; a rubber clamping plate is rotatably connected to one end of the connecting rod; a sealing cover is fixedly connected to the lower surface of the lifting frame; an electromagnetic rod is fixedly connected to the lower surface of the sealing cover; the outer surface of the lower end of the electromagnetic rod is fixedly connected with a forward rotation stirring sheet, the outer surface of the electromagnetic rod is fixedly connected with a reverse rotation stirring sheet above the forward rotation stirring sheet, and the device has the beneficial effects that a test tube is conveniently fixed, and the mixing effect of a reagent and a nucleic acid solution is conveniently improved.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA / RNA nucleic acid extraction and purification instruments, and particularly relates to a DNA / RNA nucleic acid extraction and purification instrument. Background Technique

[0002] The nucleic acid extraction and purification instrument is a high-tech product that uses the general magnetic bead method to extract nucleic acids. It has the advantages of high automation, fast extraction speed, stable results, and simple operation. Nucleic acid extraction mainly includes several main steps such as the release of intracellular contents, nucleic acid separation and purification, nucleic acid precipitation or adsorption, and the release of nucleic acids in buffer solution.

[0003] For the existing DNA / RNA nucleic acid extraction and purification instrument, during use, the test tube is generally fixed on the test tube fixing rack on the shaking rack. The traditional test tube fixing rack generally uses a spring clip for fixation. Due to the setting of the spring, the test tube is prone to detachment during shaking, and the stability is poor. Moreover, for the existing DNA / RNA nucleic acid extraction and purification instrument, during actual use, although magnetic beads are used to improve the stirring and mixing effect, the actual effect of this method is still limited. Therefore, there is an urgent need for a DNA / RNA nucleic acid extraction and purification instrument to solve the above problems. Summary of the Utility Model

[0004] (1) Technical Problems to be Solved

[0005] Aiming at the deficiencies of the prior art, the utility model provides a DNA / RNA nucleic acid extraction and purification instrument, which solves the problems put forward in the above background technique.

[0006] (2) Technical Solutions

[0007] To achieve the above object, the utility model is realized through the following technical solutions: An extraction and purification instrument for DNA / RNA nucleic acids, comprising a base, on the upper surface of the base is fixedly connected with a fixing frame, a sliding groove is opened on the front surface of the fixing frame, a motor is fixedly connected to the back surface of the fixing frame, one end of the output shaft of the motor is fixedly connected with a turntable, the front surface of the fixing frame is slidably connected with a shaking frame through a sliding block, a chute is opened on the front surface of the shaking frame, a test tube fixing frame is fixedly connected to the front surface of the shaking frame, a connecting rod is rotatably connected to the inner side wall of the test tube fixing frame, one end of the connecting rod is rotatably connected with a rubber clamping plate, a through hole is fixedly connected to the outer surface of the connecting rod, a steel wire rope penetrates through the inner side wall of the through hole, a movable plate is slidably connected to the inner side wall of the test tube fixing frame, an adjusting knob is rotatably connected to the inner side wall of the test tube fixing frame, an electric telescopic rod is fixedly connected to the outer surface of the shaking frame, one end of the telescopic rod of the electric telescopic rod is fixedly connected with a lifting frame, a sealing cover is fixedly connected to the lower surface of the lifting frame, an electromagnetic rod is fixedly connected to the lower surface of the sealing cover, a positive rotation stirring blade is fixedly connected to the outer surface of the lower end of the electromagnetic rod, and a reverse rotation stirring blade is fixedly connected to the outer surface of the electromagnetic rod above the positive rotation stirring blade. The upper surface of the base is fixedly connected with a housing, a front cover is rotatably connected to the inner side wall of the housing, a glass window is fixedly connected to the inner side wall of the front cover, a limiting block is slidably connected to the inner side wall of the front cover, a handle is fixedly connected to the outer surface of the limiting block, and a test tube rack is fixedly connected to the inner side wall of the housing.

[0008] Optionally, an eccentric shaft is fixedly connected to the front surface of the turntable, a bearing is fixedly connected to the outer surface of the eccentric shaft, and the outer surface of the bearing is slidably connected to the inner side wall of the chute.

[0009] Optionally, a connecting plate is fixedly connected to the lower surface of the connecting rod, a first spring is fixedly connected to the inner side wall of the test tube fixing frame, and one end of the first spring is fixedly connected to the outer surface of the connecting plate.

[0010] Optionally, a plurality of the connecting rods and the rubber clamping plates are provided and are evenly distributed in a circular array manner, and both ends of the steel wire rope are fixedly connected to the back surface of the movable plate.

[0011] Optionally, a plurality of the test tube fixing frames are provided and are evenly distributed in a rectangular array manner, and one end of the adjusting knob is in transmission connection with the inner side wall of the movable plate.

[0012] Optionally, a plurality of the positive rotation stirring blades and the reverse rotation stirring blades are provided and are evenly distributed in a circular array manner.

[0013] Optionally, a second spring is fixedly connected to the upper end of the limiting block, the upper end of the second spring is fixedly connected to the inner top wall of the front cover, and a card slot adapted to the lower end of the limiting block is opened on the upper surface of the housing.

[0014] The utility model provides an extraction and purification instrument for DNA / RNA nucleic acids, which has the following beneficial effects:

[0015] 1. For the extraction and purification instrument for DNA / RNA nucleic acids, through the settings of the test tube fixing rack, connecting rod, rubber clamping plate, through hole, steel wire rope, movable plate and adjusting knob, the extraction and purification instrument for DNA / RNA nucleic acids has the effect of facilitating the fixation of test tubes. Through the combined settings of the connecting rod, rubber clamping plate, through hole, steel wire rope, movable plate and adjusting knob, the test tube can be clamped and fixed at multiple angles during use, and test tubes of different sizes can be adapted, thus achieving the purpose of facilitating the fixation of test tubes.

[0016] 2. For the extraction and purification instrument for DNA / RNA nucleic acids, through the settings of the electromagnetic rod, positive rotation stirring blade and reverse rotation stirring blade, the extraction and purification instrument for DNA / RNA nucleic acids has the effect of facilitating the improvement of the mixing effect of the reagent and the nucleic acid solution. Through the combined settings of the electromagnetic rod, positive rotation stirring blade and reverse rotation stirring blade, the mixing efficiency of the reagent and the nucleic acid solution in the test tube can be effectively improved during use, thus achieving the purpose of facilitating the improvement of the mixing effect of the reagent and the nucleic acid solution. Description of the Drawings

[0017] Figure 1 is a three-dimensional structural diagram of the utility model;

[0018] Figure 2 is a three-dimensional structural diagram of the interior of the outer shell of the utility model;

[0019] Figure 3 is a side sectional structural diagram of the utility model;

[0020] Figure 4 is the utility model Figure 3 structural diagram at B in;

[0021] Figure 5 is a three-dimensional structural diagram of the fixing rack of the utility model;

[0022] Figure 6 is the utility model Figure 5 structural diagram at A in;

[0023] Figure 7 is a top three-dimensional structural diagram of the test tube fixing rack of the utility model;

[0024] Figure 8 is a front sectional structural diagram of the test tube fixing rack of the utility model;

[0025] Figure 9This is a schematic structural diagram of the electromagnetic rod of the present utility model in three dimensions.

[0026] In the figure: 1. Base; 2. Fixed frame; 3. Sliding groove; 4. Motor; 5. Turntable; 6. Eccentric shaft; 7. Shaking frame; 8. Chute; 9. Test tube fixing frame; 10. Connecting rod; 11. Connecting plate; 12. First spring; 13. Rubber clamping plate; 14. Through hole; 15. Steel wire rope; 16. Movable plate; 17. Adjusting knob; 18. Electric telescopic rod; 19. Lifting frame; 20. Sealing cover; 21. Electromagnetic rod; 22. Positive rotation stirring blade; 23. Reverse rotation stirring blade; 24. Outer shell; 25. Front cover; 26. Glass window; 27. Limit block; 28. Second spring; 29. Handle; 30. Test tube rack. Specific embodiments

[0027] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments.

[0028] Embodiment 1

[0029] The utility model provides a technical solution: an extraction and purification instrument for DNA / RNA nucleic acids, which includes a base 1. A fixed frame 2 is fixedly connected to the upper surface of the base 1. A sliding groove 3 is opened in the front of the fixed frame 2. A motor 4 is fixedly connected to the back of the fixed frame 2. One end of the output shaft of the motor 4 is fixedly connected to a turntable 5. An eccentric shaft 6 is fixedly connected to the front of the turntable 5. A bearing is fixedly connected to the outer surface of the eccentric shaft 6. The outer surface of the bearing is slidably connected to the inner side wall of the chute 8. A shaking frame 7 is slidably connected to the front of the fixed frame 2 through a sliding block. A chute 8 is opened in the front of the shaking frame 7. A test tube fixing frame 9 is fixedly connected to the front of the shaking frame 7. There are several test tube fixing frames 9 and they are evenly distributed in a rectangular array. One end of an adjusting knob 17 is in transmission connection with the inner side wall of the movable plate 16. A connecting rod 10 is rotatably connected to the inner side wall of the test tube fixing frame 9. A connecting plate 11 is fixedly connected to the lower surface of the connecting rod 10. A first spring 12 is fixedly connected to the inner side wall of the test tube fixing frame 9. One end of the first spring 12 is fixedly connected to the outer surface of the connecting plate 11. There are several connecting rods 10 and rubber clamping plates 13 and they are evenly distributed in a circular array. Both ends of a steel wire rope 15 are fixedly connected to the back of the movable plate 16. One end of the connecting rod 10 is rotatably connected to a rubber clamping plate 13. A through hole 14 is fixedly connected to the outer surface of the connecting rod 10. The steel wire rope 15 passes through the inner side wall of the through hole 14. A movable plate 16 is slidably connected to the inner side wall of the test tube fixing frame 9. An adjusting knob 17 is rotatably connected to the inner side wall of the test tube fixing frame 9. An electric telescopic rod 18 is fixedly connected to the outer surface of the shaking frame 7. One end of the telescopic rod of the electric telescopic rod 18 is fixedly connected to a lifting frame 19. A sealing cover 20 is fixedly connected to the lower surface of the lifting frame 19. An electromagnetic rod 21 is fixedly connected to the lower surface of the sealing cover 20. An outer shell 24 is fixedly connected to the upper surface of the base 1. A front cover 25 is rotatably connected to the inner side wall of the outer shell 24. A glass window 26 is fixedly connected to the inner side wall of the front cover 25. A limiting block 27 is slidably connected to the inner side wall of the front cover 25. The upper end of the limiting block 27 is fixedly connected to a second spring 28. The upper end of the second spring 28 is fixedly connected to the inner top wall of the front cover 25. A card slot adapted to the lower end of the limiting block 27 is opened on the upper surface of the outer shell 24. A handle 29 is fixedly connected to the outer surface of the limiting block 27. A test tube rack 30 is fixedly connected to the inner side wall of the outer shell 24.

[0030] In order to facilitate the fixation of test tubes, as shown in the appendix Figures 1 to 9As shown in the figure, the present application adopts the following structure. Through the settings of the test tube fixing rack 9, connecting rod 10, rubber clamping plate 13, through hole 14, steel wire rope 15, movable plate 16 and adjusting knob 17, the DNA / RNA nucleic acid extraction and purification instrument has the effect of facilitating the fixing of test tubes. During actual use, lift the handle 29 upwards. At this time, the limit block 27 is driven to slide upwards and disengages from the clamping of the clamping groove on the upper surface of the outer shell 24. Subsequently, the front cover 25 can be opened upwards. Then, the spare test tubes can be placed on the inner side wall of the test tube rack 30. After that, the test tubes that need to react are inserted into the inner side wall of the test tube fixing rack 9. Then, turn the adjusting knob 17, which drives the movable plate 16 to slide on the inner side wall of the test tube fixing rack 9, thereby pulling the two ends of the steel wire rope 15 to tighten. At this time, the steel wire rope 15 can drive multiple connecting rods 10 to rotate synchronously, and then drive multiple rubber clamping plates 13 to clamp and fix the test tubes. Moreover, during clamping, it can adapt to test tubes of different diameters, and the clamping effect is good, effectively preventing the test tubes from falling off. Subsequently, the lifting frame 19, sealing cover 20 and electromagnetic rod 21 are lowered through the electric telescopic rod 18, so that the sealing cover 20 presses on the upper end of the test tube rack 30. After that, turn on the motor 4, and the output shaft of the motor 4 drives the turntable 5 to rotate. Through the cooperative setting of the eccentric shaft 6, sliding groove 8 and sliding slot 3, the shaking frame 7 can slide up and down in front of the fixing frame 2, thereby driving the test tubes to shake up and down. That is, through the cooperative setting of the connecting rod 10, rubber clamping plate 13, through hole 14, steel wire rope 15, movable plate 16 and adjusting knob 17, the test tubes can be clamped and fixed at multiple angles during use, and can adapt to test tubes of different sizes, thus achieving the purpose of facilitating the fixing of test tubes;

[0031] Embodiment 2

[0032] The technical solution provided by the present utility model is: One end of the telescopic rod of the electric telescopic rod 18 is fixedly connected with a lifting frame 19. The lower surface of the lifting frame 19 is fixedly connected with a sealing cover 20. The lower surface of the sealing cover 20 is fixedly connected with an electromagnetic rod 21. The outer surface of the lower end of the electromagnetic rod 21 is fixedly connected with a positive rotation stirring blade 22. The outer surface of the electromagnetic rod 21 above the positive rotation stirring blade 22 is fixedly connected with a reverse rotation stirring blade 23. Both the positive rotation stirring blade 22 and the reverse rotation stirring blade 23 are provided with a plurality of them and are evenly distributed in an annular array manner.

[0033] In order to achieve the effect of facilitating the mixing of reagents and nucleic acid solutions, as shown in the appendix Figures 1 to 9As shown, the present application adopts the following structure. Through the arrangement of the electromagnetic rod 21, the positive rotation stirring blade 22, and the reverse rotation stirring blade 23, the DNA / RNA nucleic acid extraction and purification instrument is made to have the effect of facilitating the improvement of the mixing effect of the reagent and the nucleic acid solution. During actual use, after adding magnetic beads into the inside of the test tube rack 30, the retraction of the telescopic rod of the electric telescopic rod 18 drives the lifting frame 19 to descend, so that the lower surface of the sealing cover 20 abuts against the upper end of the test tube rack 30. At this time, the lower end of the electromagnetic rod 21 will insert into the inside of the test tube rack 30. During the shaking process, the magnetic beads, solution, and reagent inside the test tube rack 30 shake up and down inside. At this time, through the arrangement of the positive rotation stirring blade 22, the solution and the reagent can be rotated and disturbed. And through the arrangement of multiple reverse rotation stirring blades 23, while the solution and the reagent are being disturbed by positive rotation, they are further disturbed by reverse rotation, thereby effectively improving the stirring and mixing effect. That is, through the combined arrangement of the electromagnetic rod 21, the positive rotation stirring blade 22, and the reverse rotation stirring blade 23, during the use process, the mixing efficiency of the reagent and the nucleic acid solution in the test tube can be effectively improved, thus achieving the purpose of facilitating the improvement of the mixing effect of the reagent and the nucleic acid solution.

[0034] The above is only a preferred specific embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution of the present utility model and its inventive concept, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present utility model.

Claims

1. An extraction and purification instrument for DNA / RNA nucleic acids, comprising a base, characterized in that: The upper surface of the base is fixedly connected with a fixing frame. A sliding groove is formed in the front of the fixing frame. A motor is fixedly connected to the back of the fixing frame. One end of the output shaft of the motor is fixedly connected with a turntable. The front of the fixing frame is slidably connected with a shaking frame through a sliding block. A chute is formed in the front of the shaking frame. A test tube fixing frame is fixedly connected to the front of the shaking frame. A connecting rod is rotatably connected to the inner side wall of the test tube fixing frame. One end of the connecting rod is rotatably connected with a rubber clamping plate. A through hole is fixedly connected to the outer surface of the connecting rod. A steel wire rope penetrates through the inner side wall of the through hole. A movable plate is slidably connected to the inner side wall of the test tube fixing frame. An adjusting knob is rotatably connected to the inner side wall of the test tube fixing frame. An electric telescopic rod is fixedly connected to the outer surface of the shaking frame. One end of the telescopic rod of the electric telescopic rod is fixedly connected with a lifting frame. A sealing cover is fixedly connected to the lower surface of the lifting frame. An electromagnetic rod is fixedly connected to the lower surface of the sealing cover. A positive rotation stirring blade is fixedly connected to the outer surface of the lower end of the electromagnetic rod. A reverse rotation stirring blade is fixedly connected to the outer surface of the electromagnetic rod above the positive rotation stirring blade. The upper surface of the base is fixedly connected with a housing. A front cover is rotatably connected to the inner side wall of the housing. A glass window is fixedly connected to the inner side wall of the front cover. A limiting block is slidably connected to the inner side wall of the front cover. A handle is fixedly connected to the outer surface of the limiting block. A test tube rack is fixedly connected to the inner side wall of the housing.

2. The extraction and purification instrument for DNA / RNA nucleic acid according to claim 1, wherein: An eccentric shaft is fixedly connected to the front of the turntable. A bearing is fixedly connected to the outer surface of the eccentric shaft. The outer surface of the bearing is slidably connected to the inner side wall of the chute.

3. The nucleic acid extraction and purification instrument for DNA / RNA according to claim 1, wherein: A connecting plate is fixedly connected to the lower surface of the connecting rod. A first spring is fixedly connected to the inner side wall of the test tube fixing frame. One end of the first spring is fixedly connected to the outer surface of the connecting plate.

4. An extraction and purification instrument for DNA / RNA nucleic acids according to claim 1, characterized in that: A plurality of connecting rods and rubber clamping plates are provided and are evenly distributed in a circular array. Both ends of the steel wire rope are fixedly connected to the back of the movable plate.

5. An extraction and purification instrument for DNA / RNA nucleic acids according to claim 1, characterized in that: A plurality of test tube fixing frames are provided and are evenly distributed in a rectangular array. One end of the adjusting knob is in transmission connection with the inner side wall of the movable plate.

6. The extraction and purification instrument for DNA / RNA nucleic acid according to claim 1, wherein: A plurality of positive rotation stirring blades and reverse rotation stirring blades are provided and are evenly distributed in a circular array.

7. An extraction and purification instrument for DNA / RNA nucleic acids according to claim 1, characterized in that: A second spring is fixedly connected to the upper end of the limiting block. The upper end of the second spring is fixedly connected to the inner top wall of the front cover. A clamping groove adapted to the lower end of the limiting block is formed in the upper surface of the housing.

Images