Preparative high performance liquid chromatography pre-column

Abstract

The utility model belongs to the technical field of chromatographic separation, and particularly relates to a preparative high performance liquid chromatography pre-column, which comprises an upper sieve plate, a column tube, a lower sieve plate and a column foot, a middle column tube is positioned between the upper sieve plate and the lower sieve plate and is respectively connected with the upper sieve plate and the lower sieve plate in a sealing manner, a space for accommodating chromatographic packing is arranged in the column tube, and a sealing gasket is arranged between the upper sieve plate and the column tube. The sealing gasket is positioned above the space; the top surface of the sealing gasket is higher than the upper surface of the column pipe; the upper sieve plate is provided with a liquid inlet communicated with the space, the lower sieve plate is provided with a liquid outlet communicated with the space, and the lower sieve plate is further connected with detachable column feet. According to the utility model, the main column of the high-performance preparative liquid chromatographic column can be protected from being polluted by impurities, and the service life of the high-performance preparative liquid chromatographic column is prolonged; according to the utility model, a column assembling machine is not needed, the column can be repeatedly assembled and used, the maximum withstand voltage can reach 20Mpa, and the column efficiency, the peak shape and the separation degree of the main column are not influenced when the column is connected in series with the main column.

Description

Technical Field

[0001] The utility model belongs to the technical field of chromatographic separation, and specifically relates to a preparative high-performance liquid chromatography pre-column. Background Technique

[0002] High-performance preparative liquid chromatography is a separation technique, which is characterized by high column efficiency, large sample throughput, and less solvent consumption; however, the price of high-performance liquid chromatography columns is generally high, especially for columns with small particle size chromatographic packing materials. When using a high-performance liquid chromatography column for separation and purification experiments or preparation, the material components are complex. As the material is separated and purified, the packing material in the chromatographic column tube will be gradually contaminated, resulting in problems such as an increase in the column pressure of the chromatographic column, a decrease in the resolution, and a decrease in the adsorption capacity. To reduce the contamination of the chromatographic column packing material and extend the service life of the chromatographic column, a short column is generally added at the front end of the chromatographic column as a pre-column to filter and protect the chromatographic column, while not affecting the peak shape and separation effect of the chromatographic column.

[0003] Since the function of the pre-column is to protect the main chromatographic column, it is generally connected to the front end of the chromatographic column, and the presence of the pre-column cannot affect the peak shape and resolution of the main chromatographic column, etc.; therefore, the column height of the pre-column is generally made shorter, and its pressure resistance should also be good. Especially in the field of high-performance preparative chromatographic columns, the pre-column needs to be able to be used normally under a pressure of 10 Mpa, and some even need to be used normally under a pressure of 20 Mpa. In order for the pre-column not to affect the peak shape and separation effect of the chromatographic column, the corresponding pre-column should also be a high-pressure pre-column, and its column bed should be able to remain stable and not collapse under the impact of the high-pressure mobile phase, otherwise it will affect the peak shape and separation effect of the main column. For a pre-column with a short column height, to achieve the stability of the column bed of the pre-column under high pressure, a corresponding pressure should be applied to the column bed during column packing; and how to make the column bed of the pre-column obtain a large pressure has always been a difficult problem, especially for packing columns with packing materials with a particle size of less than 10 μm. The column packing is not tight, and under the impact of the high-pressure mobile phase, the column bed of the pre-column is very easy to collapse and form a cavity inside the pre-column, thus affecting the peak shape and separation effect of the main chromatographic column.

[0004] In the prior art, there is a pre-column of the column core type. Its principle is to press a filter sieve plate into the pre-column tube through a column packing machine device to form a column core, and then wrap and seal the column core with a housing to make a pre-column. Although this method can achieve high-pressure column packing, and the column bed of the pre-column can withstand high pressure after packing; but when packing the column, it is necessary to accurately calculate the dosage of the packing in advance. If too much packing is used, the sieve plate cannot be completely pressed into the column tube, and if too little packing is used, there will be cavities or excessive dead volume inside, which will also affect the peak shape and separation effect of the chromatographic main column. The conditions for successful column packing are extremely harsh. At the same time, for this column core type of pre-column, the column core as the core cannot be repacked; therefore, each column core can only be filled with one specification of packing and cannot be replaced, so it is extremely inflexible in actual use. Especially in the scenario application of drug R & D, the demand for pre-columns is often diverse and immediate. For each new separation sample, a new column core has to be added, which is likely to cause waste of column cores and increase the separation cost. Summary of the Invention

[0005] Aiming at the above problems existing in the existing pre-columns, the purpose of the present invention is to provide a preparative high-performance liquid chromatography pre-column. The pre-column of the present invention can not only achieve repeated column packing, but also the column bed can withstand high pressure after packing, and the column packing conditions are simple, and it can meet the requirements of the high-performance chromatographic column for connecting with the pre-column.

[0006] The purpose of the present invention is achieved by the following technical solutions:

[0007] The present invention includes an upper sieve plate, a column tube, a lower sieve plate and a column foot. The column tube is located between the upper sieve plate and the lower sieve plate and is hermetically connected to the upper sieve plate and the lower sieve plate respectively. A space for accommodating chromatographic packing is provided inside the column tube. A sealing gasket is provided between the upper sieve plate and the column tube. The sealing gasket is located above the space, and the top surface of the sealing gasket is higher than the upper surface of the column tube. An inlet is provided on the upper sieve plate and is communicated with the space. An outlet is provided on the lower sieve plate and is communicated with the space. A detachable column foot is also connected to the lower sieve plate.

[0008] Wherein: a through hole is provided in the middle of the column tube, and the through hole is the space for accommodating chromatographic packing. An inner groove is provided on the upper surface of the column tube, and the sealing gasket is accommodated in the inner groove. A plurality of threaded holes A for connecting with the upper sieve plate and the lower sieve plate are uniformly provided on the outer edge of the column tube along the circumferential direction.

[0009] The inner diameter of the sealing gasket is the same as the aperture of the through hole, and the thickness of the sealing gasket is greater than the depth of the inner groove.

[0010] Threaded holes B are evenly arranged along the circumferential direction on the outer edges of the upper sieve plate and the lower sieve plate respectively, and the threaded holes B are connected to the threaded holes A by screws; the number of the threaded holes B on the upper sieve plate is the same as that on the lower sieve plate, and both are even numbers, and the number of the threaded holes A is equal to the sum of the number of the threaded holes B on the upper sieve plate and the number of the threaded holes B on the lower sieve plate, and the threaded holes A on the column tube connected to the threaded holes B on the upper sieve plate and the threaded holes A connected to the threaded holes B on the lower sieve plate are arranged at intervals.

[0011] The upper sieve plate includes a cover plate, a distributor, a sieve mesh and a sealing ring. A groove is provided on the lower surface of the cover plate facing the column tube along the thickness direction. The distributor and the sieve mesh are respectively accommodated in the groove from top to bottom. The distributor is installed on the cover plate, the sieve mesh is installed on the distributor, and the sealing ring is located at the position where the lower surface of the cover plate facing the column tube contacts the gasket; the liquid inlet is opened on the cover plate and communicated with the groove.

[0012] The lower sieve plate includes a cover plate, a distributor, a sieve mesh and a sealing ring. A groove is provided on the upper surface of the cover plate facing the column tube along the thickness direction. The distributor and the sieve mesh are respectively accommodated in the groove from bottom to top. The distributor is installed on the cover plate, the sieve mesh is installed on the distributor, and the sealing ring is located at the position where the upper surface of the cover plate facing the column tube contacts the column tube; a column foot hole for connecting the column foot is opened on the lower surface of the cover plate; the liquid outlet is opened on the cover plate and communicated with the groove.

[0013] The advantages and positive effects of the present utility model are as follows:

[0014] 1. The upper and lower sieve plates of the present utility model and the column tube adopt a detachable design, which is flexible and convenient to use. The design of the sieve plate adds a distributor, which can effectively solve the problem of uneven distribution of the mobile phase brought about by the amplification of the analytical column to the preparative column, so that the pre-column can be used in conjunction with the high-performance chromatographic column in terms of hardware without affecting the peak shape and separation effect of the high-performance chromatographic column.

[0015] 2. The column tube structure of the present utility model, combined with the shrinkage of the gasket, conveniently and effectively solves the problem of how to obtain high pressure in the column bed inside the column tube and does not damage the sealing performance of the pre-column when the pre-column tube is generally short and there is no piston, and the column bed can always remain stable.

[0016] 3. The present utility model has obvious advantages over the prior art column core type pre-column. The pre-column can be filled with columns repeatedly, and the structure is simple and practical. There is no need to equip a professional column filling machine. The whole set of pre-columns can be used flexibly and variably, so that the efficiency is increased and the cost is reduced; the preparative high-performance liquid chromatography pre-column of the present utility model has a simple structure, is convenient and practical to operate, and has a large commercial promotion space. Description of the Drawings

[0017] Figure 1Schematic three - dimensional structure diagram of the present utility model;

[0018] Figure 2 Internal structure cross - sectional view of the present utility model;

[0019] Figure 3 is Figure 1 Schematic three - dimensional structure diagram after removing the upper sieve plate in;

[0020] Figure 4 Schematic three - dimensional structure diagram of the upper sieve plate of the present utility model;

[0021] Figure 5 Schematic three - dimensional structure diagram of the column tube of the present utility model;

[0022] Figure 6 Internal structure cross - sectional view of the column tube of the present utility model;

[0023] Figure 7 Internal structure diagram of the lower sieve plate of the present utility model;

[0024] Figure 8 Separate column efficiency test spectrogram of the C18 - 5μm packing pre - column of the present utility model;

[0025] Figure 9 Series column efficiency test spectrogram of the C18 - 5μm packing pre - column and main column of the present utility model;

[0026] Figure 10 Series column efficiency test spectrogram of the C18HCE - 5μm packing pre - column and main column of the present utility model;

[0027] Wherein: 1 is the upper sieve plate, 2 is the column tube, 3 is the lower sieve plate, 4 is the column foot, 5 is the screw, 6 is the sealing gasket, 7 is the liquid inlet, 8 is the liquid outlet, 9 is the cover plate, 10 is the distributor, 11 is the screen, 12 is the sealing ring, 13 is the inner groove, 14 is the threaded hole A, 15 is the column foot hole, 16 is the threaded hole B, 17 is the through - hole. Detailed implementation manners

[0028] The present utility model will be further described in detail below with reference to the accompanying drawings.

[0029] As Figures 1 - 7 shown, the present utility model includes an upper sieve plate 1, a column tube 2, a lower sieve plate 3 and a column foot 4. The column tube 2 is located between the upper sieve plate 1 and the lower sieve plate 3 and is hermetically connected to the upper sieve plate 1 and the lower sieve plate 3 respectively. A space for accommodating chromatographic packing is provided inside the column tube 2. A sealing gasket 6 is provided between the upper sieve plate 1 and the column tube 2. The sealing gasket 6 is located above the space, and the top surface of the sealing gasket 6 is higher than the upper surface of the column tube 2. A liquid inlet 7 communicating with the space is opened on the upper sieve plate 1, a liquid outlet 8 communicating with the space is opened on the lower sieve plate 3, and a detachable column foot 4 is further connected to the lower sieve plate 3.

[0030] The column tube 2 of this embodiment is a cylindrical tube for packing chromatographic packing; the chromatographic packing of this embodiment is chromatographic packing with a particle size of 3 to 100 microns. A through hole 17 is provided in the middle of the column tube 2, and the through hole 17 is the space for accommodating the chromatographic packing. An inner groove 13 is provided on the upper surface of the column tube 2, and the gasket 6 is accommodated in the inner groove 13; the inner diameter of the gasket 6 is the same as the aperture of the through hole 17, and the thickness of the gasket 6 is greater than the depth of the inner groove 13. The height of the column tube 2 in this embodiment is not higher than 30 mm, the depth of the inner groove 13 is less than 3 mm, the gasket 6 is an O-ring gasket, and the thickness is more than 0.2 mm thicker than the depth of the inner groove 13. The function of the gasket 6 is to make the packing protrude a small section more smoothly than the column tube 2 during column packing, and at the same time keep the protruding small section of packing from collapsing under pressure. During column packing, the upper sieve plate 1 compresses the gasket 6, and the shrinkability of the gasket 6 is used to make the corresponding pressure also applied to the protruding small section of packing, and at the same time prevent the packing from collapsing, so that the packing in the column tube 2 is in a high-pressure column packing state. A plurality of threaded holes A14 for connecting with the upper sieve plate 1 and the lower sieve plate 3 are evenly provided on the outer edge of the column tube 2 along the circumferential direction.

[0031] The upper sieve plate 1 of this embodiment is disc-shaped and includes a cover plate 9, a distributor 10, a sieve mesh 11 and a sealing ring 12. A groove is provided on the lower surface of the cover plate 9 facing the column tube 2 along the thickness direction. The distributor 10 and the sieve mesh 11 are respectively accommodated in the groove from top to bottom. The distributor 10 is embedded in the cover plate 9, the sieve mesh 11 is embedded in the distributor 10, and the sealing ring 12 is located at the position where the lower surface of the cover plate 9 facing the column tube 2 contacts the gasket 6; the liquid inlet 7 is provided at the middle position of the cover plate 9, and is opened from the upper surface of the upper sieve plate 1 to the top of the groove and communicated with the groove.

[0032] The lower sieve plate 3 of this embodiment is disc-shaped and includes a cover plate 9, a distributor 10, a sieve mesh 11 and a sealing ring 12. A groove is provided on the upper surface of the cover plate 9 facing the column tube 2 along the thickness direction. The distributor 10 and the sieve mesh 11 are respectively accommodated in the groove from bottom to top. The distributor 10 is embedded in the cover plate 9, the sieve mesh 11 is embedded in the distributor 10, and the sealing ring 12 is located at the position where the upper surface of the cover plate 9 facing the column tube 2 contacts the column tube 2; a column foot hole 15 for connecting with the column foot 4 is provided on the lower surface of the cover plate 9; the liquid outlet 8 is provided at the middle position of the cover plate 9, and is opened from the lower surface of the lower sieve plate 3 to the bottom of the groove and communicated with the groove.

[0033] The distributor 10 of this embodiment is a prior art and can adopt the distributor produced by Changzhou Ruixi Biotechnology Co., Ltd.; the function of the distributor 9 is to evenly distribute the mobile phase to the cross-section of the preparation pre-column bed to reduce the influence caused by uneven distribution of the mobile phase between the center and the edge of the pre-column.

[0034] The sieve 11 in this embodiment is a stainless steel sintered plate or other types of porous filter plates, with a filtration accuracy of 0.2 to 5 microns. The function of the sieve 11 is to block the packing and allow the sample and the mobile phase to pass through.

[0035] The sealing ring 12 in this embodiment is an O-ring, and its material is polytetrafluoroethylene. The function of the sealing ring 12 is to seal the connection between the column tube 2 and the upper sieve plate 1 and the connection between the column tube 2 and the lower sieve plate 3.

[0036] Threaded holes B16 are evenly arranged along the circumferential direction on the outer edges of the upper sieve plate 1 and the lower sieve plate 3 respectively. The threaded holes B16 are connected to the threaded holes A14 by screws 5. The number of threaded holes B16 on the upper sieve plate 1 is the same as that on the lower sieve plate 3, and both are even numbers. The number of threaded holes A14 is equal to the sum of the number of threaded holes B16 on the upper sieve plate 1 and the number of threaded holes B16 on the lower sieve plate 3. The threaded holes A14 on the column tube 2 connected to the threaded holes B16 on the upper sieve plate 1 and the threaded holes A14 connected to the threaded holes B16 on the lower sieve plate 3 are arranged at intervals. In this embodiment, 12 threaded holes A are evenly arranged along the circumferential direction on the outer edge of the column tube 2, and 6 threaded holes B16 are evenly arranged along the circumferential direction on the outer edges of the upper sieve plate 1 and the lower sieve plate 3 respectively.

[0037] The column packing method of the present utility model uses the slurry wet packing method and cooperates with negative pressure suction to suck out the slurry solvent, so that the chromatographic packing forms a column bed layer by layer from the bottom. After the column bed is filled in the pre-column, the column bed obtains high pressure in cooperation with the structural design of the pre-column. Specifically:

[0038] First, ultrasonically homogenize the chromatographic packing to be packed with a solvent, then install the lower sieve plate 3, the column tube 2 and the column foot 4 in the pre-column. Install the sealing gasket 6 in the inner groove 13 at the upper end of the column tube 2. Connect the liquid outlet 8 at the bottom of the lower sieve plate 3 to one end of the pipeline and connect it to the suction filtration vacuum pump. Gradually pour the homogenized slurry solution into the space in the column tube 2 for placing the chromatographic packing, start the suction filtration vacuum pump, so that the connecting pipeline of the liquid outlet 8 forms negative pressure, and the slurry solvent will be quickly sucked out. The packing will accumulate at the bottom of the column tube 2 and form a layer of column bed. Turn off the suction filtration vacuum pump. Continue to pour the slurry solution and start the suction filtration vacuum pump again to suck dry the solvent. Repeat the operation like this until the packing layer fills the column tube 2 and the packing layer reaches the height of the sealing gasket 6. Then use a flat scraper to scrape off the excess packing to make the packing layer flush with the sealing gasket 6. Cover the upper sieve plate 1 and gradually tighten it with screws 5 until the upper sieve plate 1 exerts sufficient pressure on the packing.

[0039] The dosage of the slurry solvent is more than 3 times the mass of the packing. The ultrasonic time of the slurry solvent is more than 5 minutes.

[0040] The upper sieve plate 1 is tightened step by step with screws 5 in a diagonal and progressive manner, and it is necessary to keep the upper sieve plate 1 horizontal during the tightening process, and the final tightening pressure needs to reach the required pressure.

[0041] Experimental Example 1

[0042] Pre-column specifications: inner diameter 50 mm, column height 10 mm; inner groove 13 has a grooving depth of 1 mm; gasket 6 has a thickness of 2 mm.

[0043] Column packing: C18CE, particle size 5 μm.

[0044] Column packing: First, weigh 18 g of C18CE packing in a beaker, pour 60 mL of ethanol, place it on an ultrasonic instrument and stir while ultrasonicating for 5 min. Install the lower sieve plate 3 of the pre-column on the column foot 4 and place it on a horizontal experimental table, then install the column tube 2 on the lower sieve plate 3, install the gasket 6 on the column tube 2, connect the liquid outlet 8 at the bottom of the lower sieve plate 3 to a vacuum pipeline, and connect the other end of the pipeline to a suction filtration vacuum pump. Carefully pour the homogenized slurry into the column tube 2, noting that the homogenized slurry should not overflow from the column tube 2. Turn on the suction filtration vacuum pump, and the homogenizing solvent quickly flows out from the liquid outlet 8, and the packing is evenly accumulated at the bottom of the column tube 2. Continue to pour the homogenized slurry to form the column bed layer by layer from the bottom of the column tube 2 until the packing layer fills the column tube 2 and reaches the height of the gasket 6. Turn off the suction filtration vacuum pump, use a flat scraper to scrape off the excess packing, make the packing interface flush with the gasket 6, cover the upper sieve plate 1, install the screws 5, and gradually tighten the screws 5 diagonally. During the tightening process, always keep the upper sieve plate 1 in a horizontal position until the screws 5 cannot be tightened further.

[0045] The test results of the pre-column alone after column packing are as Figure 8 shown.

[0046] The symmetry of the peak shape of the pre-column alone is 1.16, the column efficiency is 65000 N / m, and the test pressure is 4 Mpa.

[0047] Connect the pre-column to the high-performance chromatography main column for testing, and the test results are as Figure 9 shown.

[0048] The symmetry of the test peak shape is 1.06, the column efficiency of the high-performance chromatography main column is 83000 N / m, and the test pressure is 16.5 Mpa.

[0049] Experimental Example 2

[0050] Pre-column specifications: inner diameter 30 mm, column height 10 mm; inner groove 13 has a grooving depth of 0.5 mm; gasket 6 has a thickness of 2 mm.

[0051] Column packing: C18HCE, particle size 5 μm.

[0052] Column packing: First, weigh 5 g of C18HCE packing material in a beaker, pour 20 mL of ethanol into it, place it in an ultrasonic cleaner and stir while ultrasonicating for 8 min. Install the lower sieve plate 3 of the pre-column on the column foot 4 and place it on a horizontal experimental table. Then install the column tube 2 on the lower sieve plate 3, install the gasket 6 on the column tube 2. Connect the liquid outlet 8 at the bottom of the lower sieve plate 3 to a vacuum pipeline, and connect the other end of the pipeline to a vacuum filtration pump. Carefully pour the well-mixed slurry into the column tube 2, taking care not to let the slurry overflow from the column tube 2. Turn on the vacuum filtration pump, and the homogenizing solvent quickly flows out from the liquid outlet 8, and the packing material is evenly deposited at the bottom of the column tube 2. Continue to pour the homogenized slurry to form the column bed layer by layer from the bottom of the column tube 2 until the packing layer fills the column tube 2 and reaches the height of the gasket 6. Turn off the vacuum filtration pump, use a flat scraper to scrape off the excess packing material, making the interface of the packing material flush with the gasket 6. Cover the upper sieve plate 1, install the screw 5, and gradually tighten the screw 5 diagonally. During the tightening process, always keep the upper sieve plate 1 in a horizontal position until the screw 5 can no longer be tightened downward.

[0053] The test results of the pre-column connected to the high-performance chromatography main column after column packing are as Figure 10 shown.

[0054] After connection, the peak shape symmetry of the main column is 1.23, and the column efficiency is 95000 N / m.

[0055] Experimental Example 3

[0056] Pre-column specifications: inner diameter 50 mm, column height 30 mm; the depth of the inner groove 13 is 3 mm; the gasket 6 is 4 mm thick.

[0057] Column packing material: C18CE, particle size 5 μm.

[0058] After packing the column in the manner of Experimental Example 1, test its pressure resistance. When the test pressure reaches 3500 psi and is tested for 30 min, that is, the pressure reaches 24 Mpa, the pre-column seal is still good, there is no liquid leakage, and after disassembling the pre-column after testing, the column bed inside is still intact and there is no collapse or other situations.

[0059] The utility model belongs to an auxiliary device for a high-performance preparative liquid chromatography column, which plays a role in protecting the column before use; the pre-column can protect the main column of the high-performance preparative liquid chromatography column from impurity contamination and improve the service life of the high-performance preparative liquid chromatography column. The column packing of the utility model is simple and practical, does not require a column packing machine, can be reused for column packing, the maximum pressure resistance can reach 20 Mpa, and when used in series with the main column, it does not affect the column efficiency, peak shape and resolution of the main column.

Claims

1. A preparative high performance liquid chromatography pre-column, characterized in that: It includes an upper sieve plate (1), a column tube (2), a lower sieve plate (3) and a column foot (4). The column tube (2) is located between the upper sieve plate (1) and the lower sieve plate (3) and is hermetically connected to the upper sieve plate (1) and the lower sieve plate (3) respectively. A space for accommodating chromatographic packing is provided inside the column tube (2). A gasket (6) is provided between the upper sieve plate (1) and the column tube (2), and the gasket (6) is located above the space. The top surface of the gasket (6) is higher than the upper surface of the column tube (2). An inlet port (7) communicating with the space is provided on the upper sieve plate (1), and an outlet port (8) communicating with the space is provided on the lower sieve plate (3). A detachable column foot (4) is also connected to the lower sieve plate (3). A through hole (17) is provided in the middle of the column tube (2), and the through hole (17) is the space for accommodating chromatographic packing. An inner groove (13) is provided on the upper surface of the column tube (2), and the gasket (6) is accommodated in the inner groove (13). A plurality of threaded holes A (14) for connecting with the upper sieve plate (1) and the lower sieve plate (3) are evenly provided on the outer edge of the column tube (2) along the circumferential direction.

2. The preparative high performance liquid chromatography pre-column according to claim 1, wherein: The inner diameter of the gasket (6) is the same as the aperture of the through hole (17), and the thickness of the gasket (6) is greater than the grooving depth of the inner groove (13).

3. The preparative high performance liquid chromatography pre-column according to claim 1, wherein: Threaded holes B (16) are evenly provided on the outer edges of the upper sieve plate (1) and the lower sieve plate (3) along the circumferential direction respectively. The threaded holes B (16) are connected to the threaded holes A (14) by screws (5). The number of the threaded holes B (16) on the upper sieve plate (1) is the same as that on the lower sieve plate (3) and is an even number. The number of the threaded holes A (14) is equal to the sum of the number of the threaded holes B (16) on the upper sieve plate (1) and the number of the threaded holes B (16) on the lower sieve plate (3). The threaded holes A (14) on the column tube (2) connected to the threaded holes B (16) on the upper sieve plate (1) and the threaded holes A (14) connected to the threaded holes B (16) on the lower sieve plate (3) are arranged at intervals.

4. The preparative high performance liquid chromatography pre-column according to claim 1, wherein: The upper sieve plate (1) includes a cover plate (9), a distributor (10), a sieve mesh (11) and a sealing ring (12). A groove is provided on the lower surface of the cover plate (9) facing the column tube (2) along the thickness direction. The distributor (10) and the sieve mesh (11) are respectively accommodated in the groove from top to bottom. The distributor (10) is installed on the cover plate (9), the sieve mesh (11) is installed on the distributor (10), and the sealing ring (12) is located at the position where the lower surface of the cover plate (9) facing the column tube (2) contacts the gasket (6). The inlet port (7) is provided on the cover plate (9) and communicates with the groove.

5. The preparative high performance liquid chromatography pre-column according to claim 1, characterized in that: The lower sieve plate (3) includes a cover plate (9), a distributor (10), a sieve mesh (11) and a sealing ring (12). A groove is provided in the thickness direction on the upper surface of the cover plate (9) facing the column tube (2). The distributor (10) and the sieve mesh (11) are respectively accommodated in the groove from bottom to top. The distributor (10) is installed on the cover plate (9), the sieve mesh (11) is installed on the distributor (10), and the sealing ring (12) is located at the position where the upper surface of the cover plate (9) facing the column tube (2) contacts the column tube (2). A column foot hole (15) for connecting the column foot (4) is provided on the lower surface of the cover plate (9). The liquid outlet (8) is provided on the cover plate (9) and communicates with the groove.

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