Separated hybridization incubation box suitable for large-batch samples

Abstract

The utility model discloses a separated hybridization incubation box suitable for large-batch samples, which comprises a containing box, a sieve capable of being placed in the containing box and a cover covering an opening of the containing box, and the containing box is provided with a drain valve; the containing box is provided with supporting legs, a drainage hole is formed in the bottom of the containing box, the drainage valve comprises a valve which covers the drainage hole and can be sealed, the valve is connected with the containing box through a hinge, and the other side, away from the hinge, of the valve is connected with the containing box through a buckle. The utility model has the following beneficial effects: the drainage valve with large discharge capacity can quickly discharge liquid; according to the membrane strip washing device, a large batch of membrane strips can be rapidly filtered, washing liquid can be rapidly discharged after washing, the time of the membrane washing process is greatly shortened, and the situation that due to long-time transfer, the membrane strips are exposed in a room for a long time, non-specific reaction points are caused, and result interpretation is affected is avoided. The drain valve is convenient to open / close and simple to operate.

Description

Technical Field

[0001] The utility model relates to the field of medical devices, and particularly to a separable hybridization incubation box suitable for a large number of samples. Background Art

[0002] The detection of thalassemia is a routine detection item in the molecular group of the clinical laboratory. Currently, the most widely used technical method in the market is the reverse dot blot method. This method is cheap and the technical method is stable, suitable for large-scale clinical detection. However, the whole process of the hybridization method is cumbersome, with high temperature and technical requirements. The whole process reaction needs to be carried out at a temperature of 43.7 degrees in a constant temperature water bath. Each step will affect the final hybridization result, especially the influence of the incubation temperature during the hybridization process. Because the change of temperature will affect the denaturation and renaturation of nucleic acid molecules, resulting in non-specific sites on the membrane strip or hybridization failure, affecting the result interpretation. And the step most affected by temperature is the membrane washing process after hybridization. This process needs to transfer the membrane strip from the reaction tube containing the hybridization solution to the washing solution. Currently, the ordinary iron cans used in the laboratory do not have a filter screen and no function of retaining the membrane strip. It is necessary to use tweezers to take out the membrane strip from a large number of hybridization reaction tubes, and then put it into the washing tank for washing. The time-consuming is long during this period, and the membrane strip is exposed to room temperature for a long time, and the temperature change leads to non-specific hybridization points, affecting the result interpretation. When using an ordinary iron can to pour out the washing solution after washing, it is easy to pour out the membrane strip together, resulting in the failure of the experiment. Summary of the Utility Model

[0003] The technical problem to be solved by the utility model is to provide a separable hybridization incubation box suitable for a large number of samples, which can quickly transfer the membrane strip to the washing solution, and can also quickly drain the washing solution after washing, greatly reducing the time of the membrane washing process.

[0004] The technical solution for realizing the purpose of the utility model is: a separable hybridization incubation box suitable for a large number of samples, characterized in that: it includes a containing box, a sieve that can be put into the containing box, and a lid that covers the opening of the containing box, and the containing box is provided with a drain valve;

[0005] The containing box is provided with feet, the bottom of the containing box is provided with a drain hole, the drain valve includes a valve that covers the drain hole and can be sealed, the valve is connected to the containing box through a hinge, and the other side of the valve away from the hinge is connected to the containing box through a buckle.

[0006] A sealing rubber strip is arranged between the valve and the bottom of the containing box.

[0007] The buckle includes a latch connected to the valve and a clamping seat provided on the accommodation box. The latch includes a connecting line connected to the valve. The other end of the connecting line away from the valve is provided with a guide tube. The other end of the guide tube away from the connecting line is provided with a locking block. The side of the locking block is provided with a clamping hole. The clamping seat is provided with a spring pin matching the clamping hole. The accommodation box is provided with a guide block, and the guide block is provided with a through hole matching the guide tube.

[0008] The other end of the spring pin away from the locking block is provided with a pull rope.

[0009] Hooks are provided on the opposite sides of the sieve.

[0010] The separable hybridization incubation box suitable for a large number of samples of the present utility model has the following beneficial effects: a large-displacement drain valve can quickly discharge liquid; a large number of membrane strips can be quickly filtered, and the washing liquid can also be quickly discharged after washing, greatly reducing the time of the membrane washing process, avoiding the membrane strips being exposed to room temperature for too long during long-term transfer, thereby causing non-specific reaction points and affecting the result interpretation. Just gently pull the pull rope to open the drain valve, and lift the locking block upward to close the drain valve. The operation is simple and convenient, reducing the time for the user to open / close the drain valve, and the hand does not need to touch the liquid in the box. Description of the Drawings

[0011] The present utility model will be described in detail below in conjunction with the drawings and embodiments;

[0012] Figure 1 is a schematic structural diagram of the present utility model;

[0013] Figure 2 is a schematic structural diagram of the accommodation box of the present utility model;

[0014] Figure 3 is an exploded structural diagram of the present utility model. Detailed Embodiments

[0015] As Figures 1 to 3 shown, the separable hybridization incubation box suitable for a large number of samples includes an accommodation box 1, a sieve 2 that can be placed in the accommodation box 1, and a lid 3 that covers the opening of the accommodation box 1. Hooks 2.1 are provided on the opposite sides of the sieve 2, and the accommodation box 1 is provided with a drain valve 4.

[0016] The accommodation box 1 is provided with feet 1.1, and a drain hole 1.2 is provided at the bottom of the accommodation box 1. The drain valve 4 includes a valve 4.1 that covers and can seal the drain hole 1.2. The valve 4.1 is connected to the accommodation box 1 through a hinge 5. A sealing rubber strip 6 is provided between the valve 4.1 and the bottom of the accommodation box 1. The other side of the valve 4.1 away from the hinge 5 is connected to the accommodation box 1 through a buckle 4.2.

[0017] The buckle 4.2 includes a latch 4.3 connected to the valve 4.1 and a socket 1.3 provided on the accommodation box 1. The latch 4.3 includes a connecting line 4.4 connected to the valve 4.1. The other end of the connecting line 4.4 away from the valve 4.1 is provided with a guide tube 4.5. The other end of the guide tube 4.5 away from the connecting line 4.4 is provided with a locking block 4.6. A card hole 4.7 is provided on the side of the locking block 4.6. The socket 1.3 is provided with a spring pin 7 matching the card hole 4.7. The accommodation box 1 is provided with a guide block 1.4. The guide block 1.4 is provided with a through hole 1.5 matching the guide tube 4.5.

[0018] The other end of the spring pin 7 away from the locking block 4.6 is provided with a pull cord 8.

[0019] During use

[0020] After the membrane strip hybridization reaction is completed, open the drain valve, pour the hybridization solution and the membrane strip into the sieve together, so that the poured hybridization solution drains away quickly, and the membrane strip remains in the sieve. After the hybridization solution is filtered, close the drain valve, pour the washing solution into the accommodation box, wash after the washing solution covers all the membrane strips. After the washing is completed, open the drain valve. After the washing solution is drained, close the drain valve, add the incubation solution into the accommodation box for incubation, and cover the lid for light avoidance.

Claims

1. A separable hybridization incubation box suitable for a large number of samples, characterized in that: It includes a containing box, a sieve that can be placed in the containing box, and a lid that covers the opening of the containing box. The containing box is provided with a drain valve; The containing box is provided with feet. The bottom of the containing box is provided with a drain hole. The drain valve includes a valve that covers and seals the drain hole. The valve is connected to the containing box by a hinge. The other side of the valve away from the hinge is connected to the containing box by a buckle.

2. The separable hybridization incubation box suitable for a large number of samples according to claim 1, characterized in that: The buckle includes a lock connected to the valve and a clamping seat provided on the containing box. The lock includes a connecting line connected to the valve. The other end of the connecting line away from the valve is provided with a guide tube. The other end of the guide tube away from the connecting line is provided with a locking block. The side of the locking block is provided with a card hole. The clamping seat is provided with a spring pin matching the card hole. The containing box is provided with a guide block. The guide block is provided with a through hole matching the guide tube.

3. The separable hybridization incubation box suitable for a large number of samples according to claim 1, characterized in that: A sealing rubber strip is provided between the valve and the bottom of the containing box.

4. The separable hybridization incubation box suitable for a large number of samples according to claim 2, wherein: A pull rope is provided at the other end of the spring pin away from the locking block.

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