Sample pretreatment device

Abstract

The utility model relates to the technical field of sample detection, and discloses a sample pretreatment device which is characterized in that a turntable module for driving a test tube disc to rotate is arranged on a bottom plate, and a uniform mixing module is arranged on one side of the turntable module; the uniform mixing module comprises a nozzle seat positioned above the test tube disc, and a nozzle body for injecting a reagent into the test tube is mounted on the nozzle seat; a uniform mixing rod capable of rotating around the axis of the uniform mixing rod is arranged below the test tube disc, the uniform mixing rod can jack test tubes on the test tube disc to be connected with the nozzle main body, after a reagent is injected, the uniform mixing rod drives the test tubes to rotate so as to achieve the purpose of uniformly mixing the reagent, then the uniform mixing rod is reset, and pretreatment of a sample is completed; the test tube disc is driven to rotate by a certain angle through the rotating disc module, the same operation is performed on the next test tube, and the device is compact in structure, small in occupied space, capable of preparing abundant types of samples, capable of working continuously, free of manual intervention and high in sample preparation efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of sample detection, and particularly relates to a sample pretreatment device. Background Art

[0002] At present, the preliminary preparation work of flow cytometer reagents mainly relies on manual operation. When the sample preparation volume is large, the manual sample preparation operation process has many steps, poor accuracy, is prone to errors, and has low efficiency. Therefore, fully automatic pretreatment instruments and semi-automatic pretreatment instruments have emerged as the times require.

[0003] At present, the fully automatic pretreatment instruments on the market can realize fully automatic sample processing, but they are large in volume, heavy in weight, cannot be carried, have high environmental requirements, complex operations, and large consumption of consumables such as tip heads; the semi-automatic pretreatment instruments are relatively small in volume but have simple functions, a single type of sample to be prepared, and some work needs to be completed manually; for reagents with special requirements, they cannot be prepared.

[0004] Therefore, in order to improve the sample preparation efficiency, avoid errors in manual operation, and realize rapid sample preparation, the utility model provides a sample pretreatment device. Summary of the Utility Model

[0005] The purpose of the utility model is to overcome the deficiencies in the prior art and provide a sample pretreatment device. Through the cooperation between the mixing module and the turntable module, the reagent can be automatically injected into and mixed in the test tubes on the test tube tray, which not only has high efficiency but also can avoid errors in manual operation.

[0006] To achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] A sample pretreatment device includes a bottom plate. A turntable module for driving the rotation of the test tube tray is arranged on the bottom plate, and a mixing module is arranged on one side of the turntable module; the mixing module includes a nozzle seat located above the test tube tray, and a nozzle body for injecting reagent into the test tube is installed on the nozzle seat; a mixing rod capable of rotating around its own axis is arranged below the test tube tray, and the mixing rod can lift the test tube to be docked with the nozzle body.

[0008] Optionally, the mixing module further includes a support plate for installing the nozzle seat. A sliding plate capable of lifting is sleeved on the support plate in a sliding manner. A mixing motor is installed at one end of the sliding plate close to the test tube tray, and the lower end of the mixing rod is connected to the output end of the mixing motor.

[0009] Optionally, a lead screw parallel to the mixing rod is rotatably installed on the side of the support plate opposite to the test tube tray, and the lead screw is threadedly penetrated through the sliding plate.

[0010] Optionally, a transverse slide rail and a longitudinal slide rail are fixedly installed on the support plate. The longitudinal slide rail is parallel to the mixing rod, and the sliding plate is slidably connected to the longitudinal slide rail through a slider; the transverse slide rail is perpendicular to the longitudinal slide rail, and a waste liquid bottle seat for placing a waste liquid bottle is slidably installed on the transverse slide rail.

[0011] Optionally, a positioning groove for positioning a test tube is formed at the bottom of the nozzle seat. One end of the nozzle body extends into the positioning groove, and the other end is communicated with a reagent bottle storing a reagent.

[0012] Optionally, a weighing assembly for carrying the reagent bottle is further arranged on the bottom plate, and the weighing assembly corresponds to the position and quantity of the reagent bottles.

[0013] Optionally, an injection pump and a solenoid valve are arranged on the pipeline connecting the nozzle body and the reagent bottle.

[0014] Optionally, the turntable module includes a mounting plate connected to the bottom plate. A support platform and a driving motor are arranged on the mounting plate. A rotating shaft is penetrated through the support platform, and an upper end of the rotating shaft is connected with a fixing plate for mounting the test tube tray. A lower end of the rotating shaft is connected with an output end of the driving motor through a transmission assembly.

[0015] Optionally, a housing is covered on the bottom plate. A flip cover capable of being turned over is further arranged on the housing, and both the nozzle seat and the test tube tray are located between the housing and the flip cover.

[0016] Advantageous Effects

[0017] (1) In the utility model, the mixing rod can lift the test tubes on the test tube tray to be connected with the nozzle body. After injecting the reagent, the mixing rod drives the test tubes to rotate to achieve the purpose of mixing the reagent. Subsequently, the mixing rod resets to complete the pretreatment of the sample; then the turntable module drives the test tube tray to rotate by a certain angle, and the same operation is performed on the next test tube. The device has a compact structure, occupies a small space, can prepare a rich variety of samples, can work continuously and without manual intervention, and has a high sample preparation efficiency.

[0018] (2) For the above-mentioned mixing rod, the lifting of the test tube is realized by driving the sliding plate to lift by a lead screw, and the rotation and mixing of the reagent in the test tube are directly driven by a mixing motor. The structure is compact, greatly reducing the occupied space of the whole machine, and the operation is stable and reliable. Description of the Drawings

[0019] Figure 1 is a schematic structural diagram of the sample pretreatment device according to an embodiment of the utility model;

[0020] Figure 2It is a schematic structural diagram of the mixing module in the embodiment of the present utility model;

[0021] Figure 3 It is a schematic side view structural diagram of the mixing module in the embodiment of the present utility model;

[0022] Figure 4 It is a schematic structural diagram of the nozzle seat in the embodiment of the present utility model;

[0023] Figure 5 It is a schematic structural diagram of the turntable module in the embodiment of the present utility model;

[0024] Figure 6 It is a schematic internal structural diagram of the turntable module in the embodiment of the present utility model;

[0025] Figure 7 It is a schematic structural diagram of the liquid path module in the embodiment of the present utility model;

[0026] Figure 8 It is a schematic structural diagram of the outer shell in the embodiment of the present utility model;

[0027] Figure 9 It is a schematic positional structural diagram of the outer shell and the test tube tray in the embodiment of the present utility model;

[0028] Among them, 1. bottom plate;

[0029] 2. turntable module; 21. mounting plate; 22. support platform; 23. fixed disk; 24. rotating shaft; 25. driving motor; 26. transmission component; 27. support pillar;

[0030] 3. mixing module; 31. support plate; 32. sliding plate; 33. mixing motor; 34. mixing rod; 35. lead screw; 36. nozzle seat; 361. positioning groove; 37. horizontal slide rail; 38. waste liquid bottle seat; 39. vertical slide rail;

[0031] 4. liquid path module; 41. bracket; 42. cleaning pump; 43. injection pump; 44. solenoid valve; 45. liquid path control board; 46. power supply;

[0032] 5. test tube tray; 6. weighing component; 7. nozzle body; 8. outer shell; 81. flip cover; 82. control module; 9. reagent bottle. Detailed implementation manners

[0033] Now, the present utility model will be further described in detail with reference to the accompanying drawings and embodiments. These drawings are all simplified schematic diagrams, only illustrating the basic structure of the present utility model in a schematic manner, so they only show the components related to the present utility model.

[0034] Embodiment 1

[0035] As Figure 1As shown in the figure, a sample pretreatment device includes a turntable module 2, a mixing module 3, a liquid path module 4, and a weighing assembly 6 disposed on a bottom plate 1.

[0036] The turntable module 2 is used to drive the test tube tray 5 to rotate at a certain pitch so that each test tube on it can take turns to be in the working position to complete the pretreatment of the sample; the test tube tray 5 adopts the prior art, and the test tubes are loaded on the outer circumferential surface of the test tube tray 5, which has corresponding mounting grooves, and through holes are provided at the bottoms of the mounting grooves.

[0037] The function of the mixing module 3 is to lift the test tubes on the test tube tray 5 to complete the filling of the reagent and mix the filled reagent; the function of the liquid path module 4 is to pump the reagent from the reagent bottle 9 to the corresponding test tube.

[0038] As Figure 1 、 Figures 2 - 4 shown in the figure, the mixing module 3 is located on one side of the turntable module 2, and the mixing module 3 includes a support plate 31 fixedly installed on the bottom plate 1. A nozzle seat 36 is fixedly installed on the upper part of the support plate 31, and a nozzle body 7 for injecting the reagent into the test tube is installed on the nozzle seat 36.

[0039] A slide plate 32 is arranged below the nozzle seat 36. An opening is provided on the slide plate 32. The slide plate 32 is slidably sleeved on the support plate 31 through the opening. The support plate 31 is perpendicular to the bottom plate 1, the slide plate 32 is parallel to the bottom plate 1, and the slide plate 32 is slidably connected to the support plate 31 through a longitudinal slide rail 39 and a slider at the opening.

[0040] The front end of the slide plate 32 is close to the test tube tray 5 and a mixing motor 33 is installed. The output end of the mixing motor 33 is connected to a mixing rod 34, and the mixing rod 34 is parallel to the axis of the test tube tray 5; a lead screw 35 parallel to the mixing rod 34 is rotatably installed on the side of the support plate 31 opposite to the test tube tray 5, and the lead screw 35 is screwed through the rear end of the slide plate 32.

[0041] Among them, a positioning groove 361 for test tube positioning is provided at the bottom of the nozzle seat 36. One end of the nozzle body 7 extends into the positioning groove 361, and the other end is communicated with the reagent bottle 9 storing the reagent; and when the test tube is in the working position, the test tube is coaxial with the mixing rod 34 and the positioning groove 361.

[0042] Specifically, the nozzle seat 36 and the mixing rod 34 are respectively located on the upper and lower sides of the test tube disk 5; when the test tube rotates to be coaxial with the mixing rod 34 and the positioning groove 361, the screw rod 35 is driven to rotate by the corresponding motor, thereby driving the slide plate 32 to move up along the longitudinal slide rail 39, and the top of the mixing rod 34 passes through the through hole to contact the bottom of the test tube, and continues to move up until the test tube mouth enters the positioning groove 361 and aligns with the nozzle body 7. At this time, the test tube has not completely separated from the test tube disk 5, and then under the control of the liquid circuit module 4, the reagent in the reagent bottle 9 is injected into the test tube through the nozzle body 7; after the injection is completed, the mixing rod 34 moves down a distance, and the mixing motor 33 is started, driving the mixing rod 34 to rotate around its own axis, and then driving the test tube to rotate to mix the reagent inside it, and then the mixing rod 34 is reset, and the turntable module 2 drives the test tube disk 5 to rotate a division, so that the next test tube is in a position coaxial with the mixing rod 34 and the positioning groove 361.

[0043] The top of the mixing rod 34 is provided with a concave pit corresponding to the bottom contour of the test tube, so that after the test tube is lifted up, the test tube can be driven to complete the rotation and mixing.

[0044] like Figure 1 , Figures 7 - 9 As shown, the liquid circuit module 4 includes a bracket 41 fixedly mounted on the base plate 1 , and a cleaning pump 42 , a syringe pump 43 , a solenoid valve 44 , a liquid circuit control board 45 and a power supply 46 are fixedly mounted on the bracket 41 .

[0045] The injection pump 43 and the solenoid valve 44 are both located on the pipeline connecting the nozzle body 7 and the reagent bottle 9. The solenoid valve 44 is used to control the opening and closing of the liquid circuit. Using the injection pump 43 for adding samples can improve the sampling accuracy. The power supply 46 supplies power to the electrical components of the device, and the liquid circuit control board 45 allows the entire liquid circuit system to work in an orderly manner.

[0046] Three nozzle bodies 7 are provided, which are respectively connected to reagent bottles 9 storing different reagents, so that three different reagents can be added. The reagents can be added at one time according to the set program, thereby improving the sample addition efficiency.

[0047] A cleaning pump 42 is also provided on the pipeline connecting the nozzle body 7 and the reagent bottle 9, and a pipeline cleaning program is provided to realize automatic pipeline cleaning.

[0048] Furthermore, a transverse slide rail 37 is fixedly mounted on the support plate 31, and the transverse slide rail 37 is perpendicular to the longitudinal slide rail 39, and a waste liquid bottle seat 38 for placing a waste liquid bottle is slidably mounted on the transverse slide rail 37. The waste liquid generated by the cleaning pipeline can be discharged into the waste liquid bottle through the nozzle body 7, and the waste liquid bottle is placed on the waste liquid bottle seat 38.

[0049] When starting the pipeline cleaning program, remove the test tube tray 5, move the waste liquid bottle holder 38 along the horizontal slide rail 37 to directly below the nozzle body 7, and then place the waste liquid bottle on it to receive the waste liquid generated after cleaning.

[0050] To avoid frequent reagent replacement, the reagents of this device are placed in whole bottles, and the reagent bottle 9 is placed at the weighing position, which can realize the alarm function for insufficient reagents. The weighing position is set on the bottom plate 1, and a weighing component 6 for carrying the reagent bottle 9 is provided at the corresponding weighing position, and the weighing component 6 corresponds to the position and quantity of the reagent bottle 9.

[0051] The above-mentioned weighing component 6 includes a substrate fixedly connected to the bottom plate 1. A pedestal for supporting the reagent bottle 9 is provided above the substrate, and a pressure sensor is provided between the substrate and the pedestal; when the reagent bottle 9 is placed on the pedestal, the following sensor will calculate the remaining reagent volume, and when it is lower than the minimum liquid volume warning value, the device will give an alarm prompt to facilitate the staff to supplement or replace the reagent.

[0052] In addition, a housing 8 is covered on the bottom plate 1, and a flip cover 81 that can be flipped is also provided on the housing 8; the turntable module 2, the mixing module 3, the liquid path module 4, and the weighing component 6 are all located inside the housing 8, while the nozzle seat 36, the test tube tray 5, and the waste liquid bottle holder 38 are all located between the housing 8 and the flip cover 81. After the flip cover 81 is flipped and closed, the nozzle seat 36, the test tube tray 5, and the waste liquid bottle holder 38 can be covered.

[0053] The functions of the housing 8 and the flip cover 81 are to make the overall device have a good appearance and protect the internal modules, and a control module 82 is also provided at the front end of the housing 8. Through this module, the overall operation of the device can be controlled.

[0054] Embodiment 2

[0055] On the basis of Embodiment 1, the present invention also proposes the structure of the turntable module 2 for driving the rotation of the test tube tray 5.

[0056] As Figure 1 、 Figures 5 - 6 shown, the turntable module 2 includes a mounting plate 21 connected to the bottom plate 1. A support platform 22 and a driving motor 25 are provided on the mounting plate 21. A rotating shaft 24 is passed through the support platform 22, and the upper end of the rotating shaft 24 is connected to a fixed plate 23 for installing the test tube tray 5, and the lower end of the rotating shaft 24 is connected to the output end of the driving motor 25 through a transmission component 26.

[0057] The mounting plate 21 is fixedly connected to the support platform 22 and the bottom plate 1 through columns 27, and the three are parallel to each other. The interval between the mounting plate 21 and the bottom plate 1 is for the installation of the driving motor 25, and the interval between the mounting plate 21 and the support platform 22 is for the assembly of the transmission component 26.

[0058] The rotating shaft 24 is rotatably installed on the support platform 22. The transmission assembly 26 adopts an existing belt drive structure, including a driving pulley, a driven pulley and a synchronous belt. The driving pulley is installed at the output end of the driving motor 25, the driven pulley is installed at the lower end of the rotating shaft 24, and the synchronous belt is sleeved on the driving pulley and the driven pulley. And the diameter of the driven pulley is larger than that of the driving pulley, forming a speed reduction structure to ensure that the torque at the output end of the driving motor 25 can be stably transmitted to the rotating shaft 24.

[0059] The fixed disk 23 is fixedly installed on the top of the rotating shaft 24. When the rotating shaft 24 drives the rotation through the driving motor 25 and the transmission assembly 26, the rotating shaft 24 can drive the fixed disk 23 to rotate synchronously; a groove is provided on the outer circumferential surface of the fixed disk 23, and corresponding protrusions are provided at the bottom of the existing test tube tray 5. The two are inserted and matched, and the torque of the rotating shaft 24 can be transmitted to the test tube tray 5 to drive it to rotate synchronously.

[0060] Workflow:

[0061] 1. After power-on, the turntable is initialized. After the system self-check, it is confirmed that all parameter settings are completed, and the turntable is in a state of waiting for testing;

[0062] 2. Open the flip cover 81, take out the test tube tray 5, place the flow cytometry tubes to be prepared, and the maximum sample loading amount is 40. Then install the test tube tray 5 on the fixed disk 23 and close the flip cover 81;

[0063] 3. The driving motor 25 in the turntable module 2 rotates the test tube tray 5 through the transmission assembly 26, and places the test tube in the 1st position directly below the nozzle seat 36;

[0064] 4. The lead screw 35 drives the slide plate 32 and the mixing rod 34 to rise, pushes the test tube out of the test tube tray 5, and the test tube mouth enters the positioning groove 361 of the nozzle seat 36;

[0065] 5. The injection pump 43 adds reagents into the test tube through the nozzle body 7 according to the program setting, and then the mixing motor 33 drives for mixing; after the addition of the three reagents is completed, the slide plate 32 drives the mixing rod 34 to descend, and the test tube returns to its original position;

[0066] 6. The driving motor 25 in the turntable module 2 rotates, places the next test tube directly below the nozzle seat 36, and starts the preparation of the next sample. Repeat the above steps in sequence until the preparation of the entire tray of samples is completed;

[0067] 7. Open the flip cover 81, take out the test tube tray 5, replace the test tubes, and conduct the next round of testing.

[0068] In summary, the sample pretreatment device proposed by the present utility model can reduce manual participation and avoid errors; it has high movement precision and good repeatability; it uses a 40-well test tube turntable, which is universal with the existing test tube tray 5 of flow cytometers. After the reagent is prepared, the whole tray can be directly loaded onto the machine for rapid sampling and analysis; the whole machine is light in weight and small in size, facilitating movement and saving space.

[0069] After the operator edits the reagents and volumes to be added and then places the test tube tray 5 into the instrument and clicks the start button, no subsequent manual intervention is required. The operation is simple and easy to master, providing a good customer experience; the mechanical operation precision and efficiency are both higher than manual operation, reducing the labor intensity and accelerating the testing speed; it uses common flow cytometry tubes on the market, and the consumables are easily obtained. The structure is compact, saving space and facilitating transportation.

[0070] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation of the present utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first", "second", etc. may explicitly or implicitly include one or more of such features. In the description of the present utility model, unless otherwise stated, the meaning of "plurality" is two or more.

[0071] In the description of the present utility model, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood through specific situations.

[0072] Based on the inspiration of the ideal embodiments of the present utility model, through the above description, relevant personnel can make various changes and modifications completely within the scope without departing from the technical idea of this utility model. The technical scope of this utility model is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. A sample pretreatment device, comprising a bottom plate (1), characterized in that: A turntable module (2) for driving the rotation of the test tube tray (5) is provided on the bottom plate (1), and a mixing module (3) is provided on one side of the turntable module (2). The mixing module (3) includes a nozzle seat (36) located above the test tube tray (5), and a nozzle body (7) for injecting reagents into the test tubes is installed on the nozzle seat (36); a mixing rod (34) capable of rotating around its own axis is provided below the test tube tray (5), and the mixing rod (34) can lift the test tube to dock with the nozzle body (7).

2. The sample pretreatment device according to claim 1, wherein: The mixing module (3) further includes a support plate (31) for installing the nozzle seat (36), a slidable lifting slide plate (32) is sleeved on the support plate (31), a mixing motor (33) is installed at one end of the slide plate (32) close to the test tube tray (5), and the lower end of the mixing rod (34) is connected to the output end of the mixing motor (33).

3. The sample pretreatment device according to claim 2, wherein: A lead screw (35) parallel to the mixing rod (34) is rotatably installed on one side of the support plate (31) opposite to the test tube tray (5), and the lead screw (35) is screwed through the slide plate (32).

4. The sample pretreatment device according to claim 3, characterized in that: A transverse slide rail (37) and a longitudinal slide rail (39) are fixedly installed on the support plate (31), the longitudinal slide rail (39) is parallel to the mixing rod (34), and the slide plate (32) is slidably connected to the longitudinal slide rail (39) through a slider; the transverse slide rail (37) is perpendicular to the longitudinal slide rail (39), and a waste liquid bottle seat (38) for placing a waste liquid bottle is slidably installed on the transverse slide rail (37).

5. The sample pretreatment device according to claim 1, wherein: A positioning groove (361) for positioning the test tube is opened at the bottom of the nozzle seat (36), one end of the nozzle body (7) extends into the positioning groove (361), and the other end is communicated with a reagent bottle (9) storing reagents.

6. The sample pretreatment device according to claim 5, wherein: A weighing assembly (6) for carrying the reagent bottle (9) is further provided on the bottom plate (1), and the weighing assembly (6) corresponds to the position and quantity of the reagent bottles (9).

7. The sample pretreatment device according to claim 6, wherein: An injection pump (43) and a solenoid valve (44) are provided on the pipeline connecting the nozzle body (7) and the reagent bottle (9).

8. The sample pretreatment device according to claim 7, characterized in that: The turntable module (2) includes a mounting plate (21) connected to the bottom plate (1), a support platform (22) and a driving motor (25) are provided on the mounting plate (21), a rotating shaft (24) is passed through the support platform (22), and the upper end of the rotating shaft (24) is connected with a fixed plate (23) for installing the test tube tray (5), and the lower end of the rotating shaft (24) is connected to the output end of the driving motor (25) through a transmission assembly (26).

9. The sample pretreatment device according to any one of claims 1-8, characterized in that: A housing (8) is covered on the bottom plate (1), a flip cover (81) capable of being turned over is further provided on the housing (8), and the nozzle seat (36) and the test tube tray (5) are both located between the housing (8) and the flip cover (81).

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