Multifunctional intelligent chromatographic instrument

Abstract

The utility model discloses a multifunctional intelligent chromatographic instrument, which relates to the field of liquid chromatography column passing machines, and adopts the technical scheme that the multifunctional intelligent chromatographic instrument comprises a mobile phase eluent bottle, a liquid conveying device, a chromatographic separation column, a molecular content detector and a collecting device which are communicated in sequence, the mobile phase eluent bottle is used for providing different eluents; the liquid conveying device is used for conveying the eluent corresponding to the mobile phase eluent bottle to the chromatographic separation column in proportion; the chromatographic separation column is used for separating and purifying a compound; the molecular content detector is used for detecting the content of the separated and purified chemical components; the collecting device is used for collecting the separated and purified substances. The separation and purification of a compound can be simply and quickly realized through the common reusable glass chromatographic column, the proportion preparation of an eluent is quickly performed through the matching of the main infusion pump and the branch infusion pump, and the liquid preparation, separation, detection and collection are integrated.

Description

Technical Field

[0001] The utility model relates to the field of liquid chromatography column passing machines, in particular to a multi-functional intelligent chromatograph instrument. Background Technique

[0002] Flash chromatography is a method for separating chemical mixtures. It is also often used in what can be called rapid purification methods. Sometimes it is also called low to medium pressure chromatography. This technique can be used to separate any molecule, including small chemical molecules, biological proteins, natural compound components or synthetic compounds. It is also a method of separating chemical molecules from complex mixtures. Currently, the separation of many compounds is carried out manually.

[0003] Currently, the separation technology using flash chromatography, such as the rapid preparation chromatograph disclosed in the patent document with the publication number CN215179952U, mainly sets up a storage component for collecting waste liquid. Its function in separation and purification is very simple, with all standardized fixed connections. After the method and parameters are set, the separation process ends according to the program. There are many inconveniences in the actual separation and purification operations for unknown separation methods. For example, the pressure in the column cannot be adjusted, and test tubes are used to equally collect the elution liquid containing compound components. Some only use waste liquid pipelines for collection, which is inconvenient. It cannot automatically collect different amounts of the required component elution liquid according to on-line detection to quickly complete the separation and purification process. This situation is particularly common in the separation and refinement of residues in organic chemical synthesis. Content of the Utility Model

[0004] To solve the above technical problems, the utility model provides a multi-functional intelligent chromatograph instrument, which can quickly realize the separation and purification of compounds through a commonly used reusable glass chromatography column with simple manual or automatic operations. Through the cooperation of the main path infusion pump and the branch path infusion pump, the proportion of the elution liquid can be quickly prepared, integrating liquid preparation, separation, detection and collection. The invention can better utilize the experience of manual separation workers, improve efficiency and reduce costs.

[0005] The technical solution adopted by the utility model to solve its technical problems is: a multi-functional intelligent chromatograph instrument, including a mobile phase elution liquid bottle, an infusion device, a chromatographic separation column, a compound component content detector and a collection device that are connected in sequence;

[0006] The mobile phase elution liquid bottle is used to provide different elution liquids;

[0007] The infusion device is used to transport the elution liquid corresponding to the mobile phase elution liquid bottle to the chromatographic separation column in proportion;

[0008] The chromatographic separation column is used to separate and purify compounds;

[0009] The compound component content detector is used to detect the content of chemical components obtained by separation and purification;

[0010] The collection device is used to collect the substances obtained by separation and purification.

[0011] In this solution, the eluent in the mobile phase eluent bottle is transported to the chromatographic column according to a specific ratio through the infusion device. The compounds in the chromatographic separation column are purified and separated under the action of the eluent, and their content is detected by the compound component content detector. Finally, the collection device is used to collect various compound components, such as proteins, drugs or lead drug molecules, etc.

[0012] Preferably, the mobile phase eluent bottle includes a first elution bottle, a second elution bottle, a third elution bottle and a fourth elution bottle;

[0013] The infusion device includes a main road infusion pump, a first branch infusion pump, a second branch infusion pump, a first inlet solenoid valve, a second inlet solenoid valve, a third inlet solenoid valve and a fourth inlet solenoid valve;

[0014] The outlet end of the main road infusion pump is connected to the inlet end of the chromatographic separation column;

[0015] The inlet end of the main road infusion pump is respectively connected to the outlet ends of the first branch infusion pump and the second branch infusion pump;

[0016] The inlet end of the first branch infusion pump is connected to the first elution bottle through the first inlet solenoid valve; the inlet end of the first branch infusion pump is connected to the second elution bottle through the second inlet solenoid valve;

[0017] The inlet end of the second branch infusion pump is connected to the third elution bottle through the third inlet solenoid valve; the inlet end of the second branch infusion pump is connected to the fourth elution bottle through the fourth inlet solenoid valve.

[0018] When in use, start the inlet solenoid valve and the branch infusion pump of the corresponding eluent, and start the main road infusion pump to transport the eluent in the mobile phase eluent bottle and mix it in the pipeline, so as to automatically configure the required ratio of the eluent.

[0019] Preferably, a pressure needle valve and a pressure sensor are provided between the main road infusion pump and the chromatographic separation column. The pressure of the pipeline can be detected and adjusted.

[0020] Preferably, a selection valve is provided on the pipeline at the inlet end of the chromatographic separation column, and the selection valve is connected to the waste liquid bottle. The pipeline can be connected to the waste liquid bottle through the selection valve so as to drain the residual liquid in the pipeline.

[0021] Preferably, the chromatographic separation column includes one or more columns in parallel or series, and the liquid inlet ends of the chromatographic separation columns are input through a multi-way solenoid valve. The multi-way solenoid valve is used to control the selection of the eluent.

[0022] Preferably, a metering valve is arranged between the multi-way solenoid valve and the selection valve, and the metering valve is provided with a sampling port communicating with the outside. Other functions can be extended.

[0023] Preferably, the collection device includes an X-axis slide rail, an X-axis slider, a Y-axis slide rail, a Y-axis slider, a liquid outlet solenoid valve and a liquid outlet nozzle;

[0024] The X-axis slide rail is fixed by a bracket, the X-axis slider is slidably fitted with the X-axis slide rail, the X-axis slider is provided with a Y-axis slide rail, the Y-axis slider is slidably fitted with the Y-axis slider, the Y-axis slider is provided with a liquid outlet solenoid valve, the liquid inlet end of the liquid outlet solenoid valve is communicated with the liquid outlet end of the compound component content detector, and the liquid outlet end of the liquid outlet solenoid valve is provided with the liquid outlet nozzle.

[0025] Through the cooperation of the X-axis slide rail and the Y-axis slide rail, the liquid outlet nozzle can be transported to any point on the two-dimensional plane covered by the slide rail, so as to collect the separated and purified substances, and the collection operation is more convenient.

[0026] Preferably, a collection bottle and a collection test tube group are arranged below the collection device. So as to collect separately.

[0027] Preferably, it includes a controller and a touch screen, and the controller is electrically connected to the touch screen and each electronic device respectively. So as to realize full-automatic separation and purification with higher efficiency.

[0028] Preferably, the chromatographic separation column adopts a self-packed glass chromatography column. It can greatly reduce the cost of the separation operation process.

[0029] The beneficial effects of the present utility model:

[0030] This solution can simply and quickly realize the separation and purification of compounds, quickly prepare the proportion of the eluent through the cooperation of the main road infusion pump and the branch road infusion pump, on-line detect the content of the chemical components after separation and purification, and use the pressure needle valve and the pressure sensor to adjust and detect the pipeline pressure, and quickly collect the separated and purified liquid by using the collection device. Description of the Drawings

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only 2 of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained according to these drawings.

[0032] Figure 1 Schematic diagram of an embodiment of the present utility model;

[0033] Figure 2 Schematic diagram of the principle of an embodiment of the present utility model;

[0034] Among them, 1, chromatographic separation column; 2, touch screen; 3, metering valve; 4, selection valve; 5, three-way solenoid valve; 6, pressure needle valve; 7, controller; 8, pressure sensor; 9, main path infusion pump; 10, first branch infusion pump; 11, second branch infusion pump; 12, X-axis slide rail; 13, Y-axis slide rail; 14, liquid outlet solenoid valve; 15, collection bottle; 16, collection test tube group; 17, compound component content detector; 18, mobile phase eluent bottle; 19, waste liquid bottle; 20, first liquid inlet solenoid valve; 21, second liquid inlet solenoid valve; 22, third liquid inlet solenoid valve; 23, fourth liquid inlet solenoid valve. Specific implementation mode

[0035] In order to deepen the understanding of the present utility model, the following will further describe the present utility model in detail with reference to the drawings and embodiments. The embodiments are only used to explain the present utility model and do not limit the protection scope of the present utility model.

[0036] Embodiment

[0037] As Figure 1 shown, a multifunctional intelligent chromatograph includes a mobile phase eluent bottle 18, an infusion device, a chromatographic separation column 1, a compound component content detector 17, and a collection device that are connected in sequence; the mobile phase eluent bottle 18 is used to provide different eluents; the infusion device is used to transport the eluent corresponding to the mobile phase eluent bottle 18 to the chromatographic separation column 1 in proportion; the chromatographic separation column 1 is used to separate and purify compounds; the compound component content detector 17 is used to detect the content of the compound components obtained by separation and purification; the collection device is used to collect the separated and purified substances.

[0038] In actual separation, sometimes it is necessary to adjust the conditions of the separation eluent at any time according to the separation and detection situation, such as solvent polarity, flow rate, collection bottle 15, etc.

[0039] In this solution, the eluent in the mobile phase eluent bottle is transported to the chromatographic column according to a specific ratio through the infusion device. The compounds in the chromatographic separation column 1 are purified and separated under the action of the eluent, and the content is detected by the compound component content detector 17. Finally, the collection device collects various compound components, such as proteins, drugs or lead drug molecules, etc.

[0040] In this embodiment, the compound component content detector 17 uses an ultraviolet (UV) detector to perform on-line detection of the ultraviolet absorbance of compound components when the solution after column passing passes through the flow cell. The detected data can be connected to the control system software and stored in the database.

[0041] The mobile phase eluent bottle 18 includes a first elution bottle, a second elution bottle, a third elution bottle, and a fourth elution bottle. In actual use, the number of mobile phase eluent bottles is set according to actual needs.

[0042] The infusion device includes a main path infusion pump 9, a first branch infusion pump 10, a second branch infusion pump 11, a first inlet solenoid valve 20, a second inlet solenoid valve 21, a third inlet solenoid valve 22, and a fourth inlet solenoid valve 23; the outlet end of the main path infusion pump 9 is connected to the inlet end of the chromatographic separation column 1; the inlet end of the main path infusion pump 9 is respectively connected to the outlet ends of the first branch infusion pump 10 and the second branch infusion pump 11; the inlet end of the first branch infusion pump 10 is connected to the first elution bottle through the first inlet solenoid valve 20; the inlet end of the first branch infusion pump 10 is connected to the second elution bottle through the second inlet solenoid valve 21; the inlet end of the second branch infusion pump 11 is connected to the third elution bottle through the third inlet solenoid valve 22; the inlet end of the second branch infusion pump 11 is connected to the fourth elution bottle through the fourth inlet solenoid valve 23.

[0043] During use, start the inlet solenoid valve and the branch infusion pump of the corresponding eluent, and start the main path infusion pump 9 to transport the eluent in the mobile phase eluent bottle 18 and mix it in the pipeline, so as to automatically configure the required proportion of the eluent.

[0044] A pressure needle valve 6 and a pressure sensor 8 are arranged between the main path infusion pump 9 and the chromatographic separation column 1. The pressure of the pipeline can be detected and adjusted. The pressure needle valve 6 can adopt a manual or automatic pressure regulating valve. In this embodiment, a manual pressure regulating valve is adopted. Using the pressure needle valve 6 and the pressure sensor 8 to control the pressure in the separation column and the pipeline can achieve separation conditions of low flow rate and high column pressure, so that low-cost infusion pumps such as magnetic pumps and ceramic plunger pumps can be used.

[0045] A selection valve 4 is arranged on the pipeline at the inlet end of the chromatographic separation column 1, and the selection valve 4 is connected to the waste liquid bottle 19. The pipeline can be connected to the waste liquid bottle 19 through the selection valve 4 to drain the residual liquid in the pipeline.

[0046] The chromatographic separation column 1 includes two columns arranged in parallel, and the liquid inlet ends of the two chromatographic separation columns 1 are input through a three-way solenoid valve 5. The three-way solenoid valve 5 is used to control the selection of the eluent. In practical applications, the number of chromatographic separation columns 1 can be set according to actual needs, which can be one, two or more, and can be a sample column, a separation column or a combined separation column of multiple separation columns.

[0047] A metering valve 3 is arranged between the three-way solenoid valve 5 and the selection valve 4, and the metering valve 3 is provided with a sampling port communicating with the outside. It can be used to expand other functions.

[0048] The collection device includes an X-axis slide rail 12, an X-axis slider, a Y-axis slide rail 13, a Y-axis slider, a liquid outlet solenoid valve 14 and a liquid outlet nozzle; the X-axis slide rail 12 is fixed by a bracket, the X-axis slide rail 12 is slidably fitted with the X-axis slider, the X-axis slider is provided with a Y-axis slide rail 13, the Y-axis slide rail 13 is slidably fitted with the Y-axis slider, the Y-axis slider is provided with a liquid outlet solenoid valve 14, the liquid inlet end of the liquid outlet solenoid valve 14 is communicated with the liquid outlet end of the compound component content detector 17, and the liquid outlet end of the liquid outlet solenoid valve 14 is provided with the liquid outlet nozzle.

[0049] Through the cooperation of the X-axis slide rail 12 and the Y-axis slide rail 13, the liquid outlet nozzle can be transported to any point on the two-dimensional plane covered by the slide rail to collect the separated and purified substances.

[0050] A collection bottle 15 and a collection test tube group 16 are arranged below the collection device. For separate collection. The collection bottle 15 is a rotary evaporation flask, and the main collection method is by using a rotary evaporation flask, with test tube collection as an auxiliary.

[0051] It includes a controller 7 and a touch screen 2, and the controller 7 is electrically connected to the touch screen 2 and each electronic device respectively. To achieve full-automatic separation and purification with higher efficiency. The controller 7 is a PLC, DSC or single-chip microcomputer, which is used to control the operation of the pump, the solvent polarity, the pipeline, the sampling valve, the on-line detector, etc.; operating the touch screen 2 can be used to input parameters such as solvent polarity and flow rate, monitor the separation column pressure, etc., and can manually or automatically change the separation conditions according to the on-line detection results at any time.

[0052] The chromatographic separation column 1 uses a self-packed glass chromatography column. It can greatly reduce the cost of the separation operation process, and the self-packed glass chromatography column can be reused. Of course, in actual use, a plastic medium and low pressure chromatography column or a metal chromatography column can also be used.

[0053] This solution can simply and quickly achieve the separation and purification of compounds, with higher separation and purification efficiency, and can handle a larger amount of separation and purification at the kilogram level.

[0054] The proportion of the eluent is quickly prepared through the cooperation of the main path infusion pump 9 and the branch path infusion pump. The content of the chemical components after separation and purification is detected online. The pipeline pressure can be adjusted and detected by using the pressure needle valve 6 and the pressure sensor 8. The separated and purified liquid is quickly collected by using the collection device.

[0055] The pump in this solution can adopt any one of plunger pumps, magnetic pumps, peristaltic pumps, etc. Of course, other liquid pumps can also be used. In this embodiment, a plunger pump is adopted.

[0056] The technical features of the above embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0057] The above-described embodiments merely represent several implementation manners of the present application. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the utility model patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. A multi-functional intelligent chromatograph, characterized in that, It includes a mobile phase eluent bottle (18), an infusion device, a chromatographic separation column (1), a compound component content detector (17), and a collection device that are connected in sequence; The mobile phase eluent bottle (18) is used to provide different eluents; The infusion device is used to transport the eluent corresponding to the mobile phase eluent bottle (18) to the chromatographic separation column (1) in proportion; The chromatographic separation column (1) is used to separate and purify compounds; The compound component content detector (17) is used to detect the content of the chemical components obtained by separation and purification; The collection device is used to collect the separated and purified substances.

2. The multifunctional intelligent chromatograph instrument according to claim 1, wherein: The mobile phase eluent bottle (18) includes a first elution bottle, a second elution bottle, a third elution bottle, and a fourth elution bottle; The infusion device includes a main path infusion pump (9), a first branch infusion pump (10), a second branch infusion pump (11), and a first inlet solenoid valve (20), a second inlet solenoid valve (21), a third inlet solenoid valve (22), and a fourth inlet solenoid valve (23); The liquid outlet end of the main path infusion pump (9) is connected to the liquid inlet end of the chromatographic separation column (1); The liquid inlet end of the main path infusion pump (9) is respectively connected to the liquid outlet ends of the first branch infusion pump (10) and the second branch infusion pump (11); The liquid inlet end of the first branch infusion pump (10) is connected to the first elution bottle through the first inlet solenoid valve (20); the liquid inlet end of the first branch infusion pump (10) is connected to the second elution bottle through the second inlet solenoid valve (21); The liquid inlet end of the second branch infusion pump (11) is connected to the third elution bottle through the third inlet solenoid valve (22); the liquid inlet end of the second branch infusion pump (11) is connected to the fourth elution bottle through the fourth inlet solenoid valve (23).

3. The multifunctional intelligent chromatograph according to claim 2, wherein: A pressure needle valve (6) and a pressure sensor (8) are provided between the main path infusion pump (9) and the chromatographic separation column (1).

4. The multifunctional intelligent chromatograph instrument according to claim 3, wherein: A selection valve (4) is provided on the pipeline at the liquid inlet end of the chromatographic separation column (1), and the selection valve (4) is connected to a waste liquid bottle (19).

5. The multifunctional intelligent chromatograph according to claim 4, characterized in that: The chromatographic separation column (1) includes one or more connected in parallel or in series, and the liquid inlet ends of each chromatographic separation column (1) are input through a multi-way solenoid valve.

6. The multifunctional intelligent chromatograph instrument according to claim 5, characterized in that: A metering valve (3) is provided between the multi-way solenoid valve and the selection valve (4), and the metering valve (3) is provided with a sampling port communicating with the outside.

7. The multifunctional intelligent chromatograph instrument according to claim 1, wherein: The collection device includes an X-axis slide rail (12), an X-axis slider, a Y-axis slide rail (13), a Y-axis slider, an outlet solenoid valve (14), and an outlet nozzle; The X-axis slide rail (12) is fixed by a bracket, the X-axis slide rail (12) is slidably fitted with the X-axis slider, the X-axis slider is provided with a Y-axis slide rail (13), the Y-axis slide rail (13) is slidably fitted with the Y-axis slider, the Y-axis slider is provided with an outlet solenoid valve (14), the liquid inlet end of the outlet solenoid valve (14) is connected to the liquid outlet end of the compound component content detector (17), and the liquid outlet end of the outlet solenoid valve (14) is provided with the outlet nozzle.

8. The multifunctional intelligent chromatograph according to claim 1, characterized in that: A collection bottle (15) and a collection test tube group (16) are provided below the collection device.

9. The multi-functional intelligent chromatograph according to claim 1, wherein: It includes a controller (7) and a touch screen (2), and the controller (7) is electrically connected to the touch screen (2) and each electronic device respectively.

10. The multifunctional intelligent chromatograph instrument according to claim 1, wherein: The chromatographic separation column (1) uses a self-packed glass chromatography column.

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