Multifunctional microplate reader

Abstract

The utility model relates to the technical field of microplate readers, and provides a multifunctional microplate reader which comprises a rack with a plurality of holes, and an absorbed light detection assembly, a microwell plate tray and a fluorescence detection assembly which are arranged on the rack, the rack comprises a lower layer, a middle layer and an upper layer, one part of the absorbed light detection assembly is installed in the lower layer, the other part is installed in the middle layer, the microwell plate tray is arranged in the middle layer and located on the inner side of the absorbed light detection assembly, and the fluorescence detection assembly is installed in the upper layer; the utility model has the beneficial effects that the absorption light detection component and the fluorescence detection component can share the middle layer as a sample detection layer, so that the space on the rack is reasonably utilized, and convenience is provided for a user to place an instrument; compared with the prior art that the absorption light detection assembly and the fluorescence detection assembly are distributed on the same layer, the distance between the sample and the detection assembly is shortened, and the detection effect is better.

Description

Technical Field

[0001] The utility model relates to the technical field of microplate readers, and relates to a multifunctional microplate reader. Background Art

[0002] The multifunctional microplate reader, also known as a multifunctional microplate tray detector (machine) or a multifunctional microplate tray detection platform, can provide various detection modes for experiments using a microplate tray as a system. Generally, a multifunctional microplate reader refers to an instrument with two or more detection functions. Usually, it can at least provide two most common detection functions, namely "fluorescence" and "absorbance". An independent optical path combination realizes multifunctional measurement in one instrument.

[0003] The technical principle is as follows:

[0004] Fluorescence detection uses an external light source to excite a sample at a specific wavelength. The fluorescent group is excited by an appropriate wavelength, and the molecule is converted from the ground state to the excited state. As the molecule returns to the ground state, the energy is released in the form of heat (energy loss) and light with lower energy and longer wavelength. The fluorescent group then emits an emission light with a different wavelength, which enters the detector PMT through a filter or a monochromator. The photomultiplier tube (PMT) can detect the fluorescence value of this emission, and the fluorescence intensity of the sample is expressed in relative fluorescence intensity units (RFU).

[0005] Absorbance detection uses the light wave emitted by a light source lamp to become a beam of monochromatic light through another filter or monochromator, and enters the sample to be measured in a plastic microplate tray. Part of the light is absorbed by the sample, and the other part passes through the sample and irradiates onto a photodetector. The photodetector converts the light signals with different intensities of different samples to be measured into corresponding electrical signals, and then after signal processing such as pre-amplification, logarithmic amplification, and analog-to-digital conversion, it is sent to a microprocessor for data processing and calculation, and finally the result is displayed.

[0006] In the prior art, the fluorescence detection component and the absorbance detection component in the multifunctional microplate reader are arranged in the same layer, which will cause the multifunctional microplate reader to occupy a large space and is not suitable for users with limited space. Moreover, it will cause slow single-channel absorbance detection speed and poor detection effect. Summary of the Utility Model

[0007] In view of the above technical problems existing in the prior art, a multifunctional microplate reader is provided. The absorbance detection component and the fluorescence detection component can share the middle layer as the detection layer of the sample, reasonably utilizing the space on the rack and providing convenience for users to place the instrument. Compared with the arrangement in the prior art where the absorbance detection component and the fluorescence detection component are arranged in the same layer, the distance between the sample and the detection component in this application is shortened, and the detection effect is better.

[0008] The purpose and effect of the utility model are achieved by the following specific technical means:

[0009] A multifunctional microplate reader comprises a frame with a plurality of holes, and an absorption light detection component, a microplate tray and a fluorescence detection component installed on the frame;

[0010] The rack includes a lower layer, a middle layer and an upper layer. A part of the absorption light detection component is installed in the lower layer, and another part is installed in the middle layer. The microplate tray is arranged in the middle layer and located inside the absorption light detection component. The fluorescence detection component is installed in the upper layer.

[0011] Optionally, the absorption light detection component includes a light source, an absorption light wheel, an optical fiber, an absorption light fixture and a mounting component, the light source is fixed to the lower layer, the absorption light wheel is mounted on one side of the light source, and a filter is mounted on the absorption light wheel, the absorption light fixture is inserted and fixed between the lower layer and the middle layer, and a plano-concave lens is mounted inside the absorption light fixture, the optical fiber is mounted between the absorption light wheel and the absorption light fixture, the mounting component is mounted in the middle layer, and an integrating sphere is mounted inside the mounting component, a photocell is mounted on the upper surface of the mounting component, and the microplate tray is located between the absorption light fixture and the mounting component.

[0012] Optionally, a bracket is fixed in the upper layer;

[0013] The fluorescence detection component includes a photomultiplier tube, a second light source, a first motor, an excitation wheel, a second motor and a transmitting wheel. The photomultiplier tube and the second light source are both installed in the upper layer, and the second light source is located at the input end of the photomultiplier tube. The first motor and the second motor are both fixed on a bracket. The excitation wheel and the transmitting wheel are both rotatably connected to the bracket, and the excitation wheel is transmission-connected to the output end of the first motor, and the transmitting wheel is transmission-connected to the output end of the second motor.

[0014] Optionally, a sample placement area is provided on the top of the microplate tray, and a reference channel is provided in the microplate tray next to the sample placement area, and the reference channel is used to pass the light emitted by the reference optical fiber.

[0015] Optionally, the optical fiber is a 13-channel optical fiber.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] 1. In this multifunctional microplate reader, since the microplate tray is set in the middle layer, the absorption light detection component and the fluorescence detection component can share the middle layer as the sample detection layer, which makes reasonable use of the space on the rack and provides convenience for users to place the instrument;

[0018] 2. The light source in the fluorescence detection component is above the sample, while the light source in the absorption light detection component is below the sample. Compared with the prior art where the absorption light detection component and the fluorescence detection component are arranged in the same layer, the distance between the sample and the detection component in this application is shortened, and the detection effect is better. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic perspective view of the overall structure of the present utility model;

[0020] Figure 2 is a schematic front view of the overall structure of the present utility model;

[0021] Figure 3 is a schematic side sectional view of the microplate tray of the present utility model.

[0022] Reference numerals in the figures: frame 1, lower layer 11, middle layer 12, upper layer 13, absorption light detection component 2, light source one 21, absorption light wheel 22, optical fiber 23, absorption light fixing member 24, mounting member 25, fluorescence detection component 3, photomultiplier tube 31, light source two 32, motor one 33, excitation wheel 34, motor two 35, emission wheel 36, microplate tray 4, sample placement area 41, reference channel 42, bracket 5. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] Please refer to Figures 1-3 , for a further description of the embodiments of the present utility model;

[0024] A multifunctional microplate reader includes a frame 1 with a plurality of holes and an absorption light detection component 2, a microplate tray 4, and a fluorescence detection component 3 installed on the frame 1;

[0025] The microplate tray 4 is used to place the sample to be detected, the absorption light detection component 2 is used to detect the absorbance value of the sample, and the fluorescence detection component 3 is used to detect the fluorescence value of the sample.

[0026] The frame 1 includes a lower layer 11, a middle layer 12, and an upper layer 13. A part of the absorption light detection component 2 is installed in the lower layer 11, and another part is installed in the middle layer 12. The microplate tray 4 is arranged in the middle layer 12 and is located inside the absorption light detection component 2. The fluorescence detection component 3 is installed in the upper layer 13;

[0027] Combined with Figures 1-2, the sample to be detected can be placed on the microplate tray 4. At this time, the absorption light detection component 2, the sample, and the fluorescence detection component 3 are longitudinally distributed, and both the absorption light detection component 2 and the fluorescence detection component 3 can detect the sample at a close distance. Compared with the prior art where the absorption light detection component 2 and the fluorescence detection component 3 are arranged in the same layer, the detection effect is better. Moreover, the absorption light detection component 2 and the fluorescence detection component 3 share the middle layer 12 as the detection layer of the sample, making reasonable use of the space inside the frame 1;

[0028] When the absorption light detection component 2 detects, it emits light and vertically passes through the microplate tray 4 and the sample, and then processes the light to convert the light energy into electrical energy to obtain the absorbance value of the sample;

[0029] When the fluorescence detection component 3 detects, it emits light and passes through the perforated frame 1 and irradiates the sample on the microplate tray 4. After being excited, the sample emits fluorescence to the fluorescence detection component 3, and the fluorescence value of the sample is obtained after being processed by the fluorescence detection component 3.

[0030] Compared with the prior art, the advantages of this application are as follows:

[0031] 1. Since the microplate tray 4 is arranged in the middle layer 12, the absorption light detection component 2 and the fluorescence detection component 3 can share the middle layer 12 as the detection layer of the sample, making reasonable use of the space on the frame 1 and providing convenience for users to place instruments;

[0032] 2. The light source in the fluorescence detection component 3 is above the sample, while the light source in the absorption light detection component 2 is below the sample. Compared with the prior art where the absorption light detection component 2 and the fluorescence detection component 3 are arranged in the same layer, this application shortens the distance between the sample and the detection component, and the detection effect is better.

[0033] The absorption light detection component 2 includes a light source 21, an absorption light wheel 22, an optical fiber 23, an absorption light fixing member 24, and a mounting member 25. The light source 21 is fixed to the lower layer 11. The absorption light wheel 22 is installed on one side of the light source 21, and a filter is installed on the absorption light wheel 22. The absorption light fixing member 24 is inserted and fixed between the lower layer 11 and the middle layer 12, and a plano-concave lens is installed inside the absorption light fixing member 24. The optical fiber 23 is installed between the absorption light wheel 22 and the absorption light fixing member 24, and the optical fiber 23 is a 13-channel optical fiber. The mounting member 25 is installed in the middle layer 12, and an integrating sphere is installed inside the mounting member 25. A photovoltaic cell is installed on the upper surface of the mounting member 25. The microplate tray 4 is located between the absorption light fixing member 24 and the mounting member 25;

[0034] A sample placement area 41 is provided at the top of the microplate tray 4, and a reference channel 42 is provided in the microplate tray 4 beside the sample placement area 41. The reference channel 42 is used for the light emitted by the reference optical fiber 23.

[0035] The working principle of the absorption light detection component 2 is as follows: The light generated by the light source one 21 passes through the filter on the absorption light wheel 22 for spectral splitting to become corresponding wavelengths. The light passes through the optical fiber 23 to become 13 beams of absorption light. After being focused by the plano-convex lens in the absorption light fixing member 24, 12 beams of absorption light enter the sample, and 1 beam of absorption light enters the reference channel 42. After the light passes through the sample and the reference channel 42, the integrating sphere in the mounting member 25 focuses the light, and then enters the photovoltaic cell to be converted from light energy to electrical energy, obtaining the absorbance value of the sample and the absorption photometric value of the reference. Then, the sample result is corrected using the reference result. It should be noted that the use of the absorption light detection component 2 is based on the prior art.

[0036] A bracket 5 is fixed in the upper layer 13.

[0037] The fluorescence detection component 3 includes a photomultiplier tube 31, a light source two 32, a motor one 33, an excitation wheel 34, a motor two 35, and an emission wheel 36. The photomultiplier tube 31 and the light source two 32 are both installed in the upper layer 13, and the light source two 32 is located at the input end of the photomultiplier tube 31. The motor one 33 and the motor two 35 are both fixed on the bracket 5. The excitation wheel 34 and the emission wheel 36 are both rotatably connected to the bracket 5, and the excitation wheel 34 is drivingly connected to the output end of the motor one 33, and the emission wheel 36 is drivingly connected to the output end of the motor two 35.

[0038] The working principle of the fluorescence detection component 3 is as follows: Two groups of filters are provided on the bracket 5 and are respectively facing the excitation wheel 34 and the emission wheel 36. The light generated by the light source two 32 passes through the filter on the excitation wheel 34 as excitation light and irradiates the sample on the microplate tray 4. The sample is excited to emit emission light, which passes through the filter on the emission wheel 36 and enters the photomultiplier tube 31 to obtain the fluorescence value of the sample. The motor one 33 is used to drive the excitation wheel 34 to adjust or control the selection of the excitation filter for specific optical measurements. The motor two 35 is used to drive the emission wheel 36 to adjust or select different emission filters to change the nature of the light reaching the photomultiplier tube 31. It should be noted that the use of the fluorescence detection component 3 is based on the prior art.

Claims

1. A multifunctional microplate reader, comprising a frame (1) with a number of holes, an absorption light detection component (2), a microplate tray (4) and a fluorescence detection component (3) mounted on the frame (1), characterized in that: The frame (1) includes a lower layer (11), a middle layer (12) and an upper layer (13). A part of the absorption light detection component (2) is mounted in the lower layer (11), and another part is mounted in the middle layer (12). The microplate tray (4) is arranged in the middle layer (12) and is located inside the absorption light detection component (2). The fluorescence detection component (3) is mounted in the upper layer (13).

2. The multifunctional microplate reader according to claim 1, characterized in that: The absorption light detection component (2) includes a first light source (21), an absorption light wheel (22), an optical fiber (23), an absorption light fixing member (24) and a mounting member (25). The first light source (21) is fixed to the lower layer (11). The absorption light wheel (22) is mounted on one side of the first light source (21), and a filter is mounted on the absorption light wheel (22). The absorption light fixing member (24) is inserted and fixed between the lower layer (11) and the middle layer (12), and a plano-concave lens is mounted inside the absorption light fixing member (24). The optical fiber (23) is mounted between the absorption light wheel (22) and the absorption light fixing member (24). The mounting member (25) is mounted in the middle layer (12), and an integrating sphere is mounted inside the mounting member (25). A photovoltaic cell is mounted on the upper surface of the mounting member (25). The microplate tray (4) is located between the absorption light fixing member (24) and the mounting member (25).

3. A multifunctional microplate reader according to claim 1, wherein: A bracket (5) is fixed in the upper layer (13); The fluorescence detection component (3) includes a photomultiplier tube (31), a second light source (32), a first motor (33), an excitation wheel (34), a second motor (35) and an emission wheel (36). The photomultiplier tube (31) and the second light source (32) are both mounted in the upper layer (13), and the second light source (32) is located at the input end of the photomultiplier tube (31). The first motor (33) and the second motor (35) are both fixed to the bracket (5). The excitation wheel (34) and the emission wheel (36) are both rotatably connected to the bracket (5), and the excitation wheel (34) is in transmission connection with the output end of the first motor (33). The emission wheel (36) is in transmission connection with the output end of the second motor (35).

4. The multifunctional microplate reader according to claim 2, wherein: A sample placing area (41) is provided at the top of the microplate tray (4), and a reference channel (42) is provided in the microplate tray (4) beside the sample placing area (41). The reference channel (42) is used for the light emitted by the reference optical fiber (23).

5. The multifunctional microplate reader according to claim 2, characterized in that: The optical fiber (23) is a 13-channel optical fiber.

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