Magnetic cleaning and separating device for single-molecule immunity detector

Abstract

The utility model discloses a magnetic cleaning and separating device for a single molecule immunity detector, which comprises a base, a stand column is mounted at the top of the base, a rotating plate is rotatably connected to the top of the stand column, and two centrifugal tubes for mixing magnetic beads and cleaning fluid are mounted on the rotating plate. According to the magnetic bead cleaning device, magnetic beads and cleaning liquid are poured into the centrifugal tubes, stirring blades are used for stirring and mixing, and after the rotating plate rotates, the centrifugal tubes are attached to the magnet pieces, so that the magnetic beads and the cleaning liquid are separated from each other, and the magnetic beads and the cleaning liquid are separated from each other. The magnetic beads are adsorbed by the magnet sheet, mixed liquid is pumped out through the pipettor, although many steps are carried out, operation on the device is very convenient, manual stirring and pipetting are not needed, meanwhile, the two centrifugal tubes can be used for cleaning the two sets of magnetic beads at the same time, and mutual interference is avoided; therefore, the cleaning efficiency of the magnetic beads of the single-molecule immunodetector is effectively improved.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic bead magnetic cleaning of single molecule immunoassay instruments, and specifically relates to a magnetic cleaning and separation device for single molecule immunoassay instruments. Background Technique

[0002] In a single molecule immunoassay instrument, magnetic beads, as important separation and enrichment tools, play a crucial role in the accuracy and reliability of experimental results. Therefore, the cleaning process of magnetic beads is particularly critical. Magnetic bead magnetic cleaning is an efficient cleaning technique that, based on the characteristics of magnetic particles (magnetic beads), removes dirt and impurities through the action of a magnetic field.

[0003] Currently, in order to improve the cleaning effect of magnetic beads, it is necessary to repeatedly mix and stir the magnetic beads with the cleaning solution several times, and then use magnetic force to adsorb the magnetic beads after pipetting. The operation volume is large and the efficiency is low.

[0004] Therefore, a magnetic cleaning and separation device for single molecule immunoassay instruments is proposed to solve the above problems. Content of the Utility Model

[0005] 1. Technical Problems to be Solved by the Utility Model

[0006] Aiming at the deficiencies of the existing technology, the purpose of the present utility model is to provide a magnetic cleaning and separation device for single molecule immunoassay instruments, aiming to solve the problems in the existing technology that in order to improve the cleaning effect of magnetic beads, it is necessary to repeatedly mix and stir the magnetic beads with the cleaning solution several times, and then use magnetic force to adsorb the magnetic beads after pipetting. The operation volume is large and the efficiency is low.

[0007] 2. Technical Solution

[0008] To achieve the above purpose, the present utility model provides the following technical solution:

[0009] A magnetic cleaning and separation device for single molecule immunoassay instruments includes a base. A column is installed on the top of the base. A rotating plate is rotatably connected to the top of the column. Two centrifuge tubes for mixing magnetic beads and the cleaning solution are installed on the rotating plate. A magnet sheet is installed on one side surface of the column and is attached to one side surface of one of the centrifuge tubes. An electric push rod is installed on the top of the rotating plate. A liquid transfer and mixing mechanism is installed on the electric push rod. A pipette and a stirring blade are respectively installed on the liquid transfer and mixing mechanism. The liquid transfer and mixing mechanism can extend the pipette and the stirring blade into the interior of the centrifuge tube through rotation and lifting. One end of the stirring blade is connected to a rotating rod. The top of the rotating rod is connected to the rotating shaft of a motor. The motor drives the stirring blade to rotate through the rotating rod.

[0010] As a preferred embodiment of the present utility model, magnet sheets are embedded on one side of the top of the base and on one side of the column, and the two magnet sheets are connected together.

[0011] As a preferred embodiment of the present utility model, the electric push rod is rotatably connected to the top of the column, and a circular hole is formed in the top of the rotating plate and sleeved on the outer ring surface of the electric push rod. The liquid transfer and mixing mechanism includes a support frame, and the support frame is installed on the top of the electric push rod.

[0012] As a preferred embodiment of the present utility model, through holes are symmetrically formed at both ends of the rotating plate. An edge baffle with a size larger than the through hole is provided at the top of the centrifuge tube. After the centrifuge tube is inserted into the through hole, its bottom just touches the top of the base. When the rotating plate rotates 180° on the column, the positions of the two centrifuge tubes can be alternated.

[0013] As a preferred embodiment of the present utility model, the top of the support frame is a right-angled baffle. A pipette and a motor are respectively installed at both ends of the baffle. The pipette is clamped on the support frame and the liquid suction tube is located below the baffle.

[0014] 3. Beneficial effects

[0015] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0016] In the present utility model, magnetic beads and cleaning liquid are poured into the centrifuge tube, and stirred and mixed by the stirring blade. After the rotating plate rotates, the centrifuge tube fits with the magnet sheet, and the magnet sheet adsorbs the magnetic beads. At the same time, the mixed liquid is drawn out by the pipette. Although the steps are more, the operation on this device is very convenient, without manual stirring and liquid transfer. At the same time, two centrifuge tubes can be used to clean two groups of magnetic beads simultaneously without interference, thereby effectively improving the efficiency of magnetic bead cleaning of the single molecule immunoassay detector. Description of the drawings

[0017] Figure 1 It is a schematic diagram of the overall structure of a magnetic cleaning and separation device for a single molecule immunoassay detector of the present utility model;

[0018] Figure 2 It is a schematic diagram of the unfolded structure of the column and the rotating plate of a magnetic cleaning and separation device for a single molecule immunoassay detector of the present utility model;

[0019] Figure 3 It is a schematic diagram of the structure of the liquid transfer and mixing mechanism of a magnetic cleaning and separation device for a single molecule immunoassay detector of the present utility model.

[0020] In the figure: 1. Base; 2. Column; 21. Electric push rod; 22. Magnet sheet; 3. Rotating plate; 31. Through hole; 32. Centrifuge tube; 4. Liquid transfer and mixing mechanism; 41. Support frame; 42. Motor; 43. Pipette; 44. Rotating rod; 45. Stirring blade. Detailed implementation mode

[0021] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0022] Embodiment:

[0023] Please refer to Figures 1 - 3 , this embodiment provides a magnetic cleaning and separation device for a single molecule immunoassay instrument, including a base 1. A column 2 is installed on the top of the base 1. A rotating plate 3 is rotatably connected to the top of the column 2. Two centrifuge tubes 32 for mixing magnetic beads and cleaning liquid are installed on the rotating plate 3. A magnet sheet 22 is installed on one side surface of the column 2 and is attached to one side surface of one of the centrifuge tubes 32. An electric push rod 21 is installed on the top of the rotating plate 3. A liquid transfer and mixing mechanism 4 is installed on the electric push rod 21. A pipette 43 and a stirring blade 45 are respectively installed on the liquid transfer and mixing mechanism 4. The liquid transfer and mixing mechanism 4 can extend the pipette 43 and the stirring blade 45 into the centrifuge tube 32 through rotation and lifting. One end of the stirring blade 45 is connected to a rotating rod 44. The top of the rotating rod 44 is connected to the rotating shaft of the motor 42. The motor 42 drives the stirring blade 45 to rotate through the rotating rod 44. When the magnetic cleaning and separation device for the single molecule immunoassay instrument is in use, the magnetic beads and the cleaning liquid are poured into the centrifuge tube 32, and the stirring blade 45 is used for stirring and mixing. After the rotating plate 3 rotates, the centrifuge tube 32 is attached to the magnet sheet 22, and the magnet sheet 22 is used to adsorb the magnetic beads. At the same time, the mixed liquid is drawn out through the pipette 43. Although there are many steps, the operation on this device is very convenient, without manual stirring and liquid transfer. At the same time, there are two centrifuge tubes 32 that can clean two groups of magnetic beads simultaneously without interference, thereby effectively improving the efficiency of magnetic bead cleaning of the single molecule immunoassay instrument.

[0024] In this embodiment, as Figure 1 and Figure 2 shown, the installation groove 101 is a stepped groove structure. Magnet sheets 22 are embedded and installed on one side of the top of the base 1 and one side surface of the column 2, and the two magnet sheets 22 are connected together, so that the side and bottom of the centrifuge tube 32 can be adsorbed by magnetic force to prevent the magnetic beads from being sucked into the pipette 43.

[0025] In this embodiment, as Figure 1 , Figure 2 and Figure 3 show, the electric push rod 21 is rotatably connected to the top of the column 2, and a round hole is formed in the top of the rotating plate 3 and sleeved on the outer ring surface of the electric push rod 21. The liquid transfer and mixing mechanism 4 includes a support frame 41, and the support frame 41 is installed on the top of the electric push rod 21. Therefore, both the liquid transfer and mixing mechanism 4 and the rotating plate 3 can rotate freely without interfering with each other. At the same time, the electric push rod 21 can drive the liquid transfer and mixing mechanism 4 to move up and down, so that the pipette 43 and the stirring blade 45 can enter the inside of the centrifuge tube 32.

[0026] In this embodiment, as Figure 1 and Figure 2 show, through holes 31 are symmetrically formed at both ends of the rotating plate 3. The top of the centrifuge tube 32 is provided with an edge baffle with a size larger than that of the through hole 31. After the centrifuge tube 32 is inserted into the through hole 31, its bottom just touches the top of the base 1. When the rotating plate 3 rotates 180° on the column 2, the positions of the two centrifuge tubes 32 can be alternated, thus eliminating the step of manually transferring the mixed magnetic beads and cleaning solution into the container on the magnetic rack.

[0027] In this embodiment, as Figure 1 and Figure 3 show, the top of the support frame 41 is a right-angled baffle. A pipette 43 and a motor 42 are respectively installed at both ends of the baffle. The pipette 43 is clamped on the support frame 41 and the liquid suction tube is located below the baffle. This structural design can ensure that when operating on one centrifuge tube 32, the other centrifuge tube 32 will not be interfered by the positions of the pipette 43 and the stirring blade 45.

[0028] Working principle: When the magnetic cleaning and separation device of the single-molecule immunoassay detector is in use, first, the magnetic beads to be cleaned and the cleaning solution are placed into the centrifuge tube 32. Then, the electric push rod 21 drives the support frame 41 to move downward, so that the stirring blade 45 extends into the centrifuge tube 32 to stir the mixed solution. Then, the rotating plate 3 is rotated to make the centrifuge tube 32 fit with the magnet sheet 22 on one side of the column 2, thus eliminating the step of manually transferring the mixed magnetic beads and cleaning solution into the container on the magnetic rack. The magnetic beads in the mixed solution are attracted by magnetic force. Then, the pipette 43 is used to extend into the centrifuge tube 32 to extract the cleaning solution and impurity mixed solution. Although there are many steps, the operation on this device is very convenient, without manual stirring and liquid transfer. At the same time, two centrifuge tubes 32 can be used to clean two groups of magnetic beads simultaneously without interference, thus effectively improving the efficiency of magnetic bead cleaning of the single-molecule immunoassay detector.

[0029] All technical features in this embodiment can be freely combined according to actual needs.

[0030] The above embodiments are preferred implementation solutions of the present utility model. In addition, the present utility model can also be implemented in other ways. Any obvious replacement without departing from the concept of the technical solution is within the protection scope of the present utility model.

Claims

1. A magnetic cleaning and separation device for a single molecule immunoassay detector, comprising a base (1), characterized in that: A column (2) is installed on the top of the base (1). A rotating plate (3) is rotatably connected to the top of the column (2). Two centrifuge tubes (32) for mixing magnetic beads and cleaning liquid are installed on the rotating plate (3). A magnet sheet (22) is installed on one side surface of the column (2) and is attached to one side surface of one of the centrifuge tubes (32). An electric push rod (21) is installed on the top of the rotating plate (3). A liquid transfer and mixing mechanism (4) is installed on the electric push rod (21). A pipette (43) and a stirring blade (45) are respectively installed on the liquid transfer and mixing mechanism (4). The liquid transfer and mixing mechanism (4) can extend the pipette (43) and the stirring blade (45) into the interior of the centrifuge tube (32) by rotation and lifting. One end of the stirring blade (45) is connected to a rotating rod (44). The top of the rotating rod (44) is connected to the rotating shaft of the motor (42). The motor (42) drives the stirring blade (45) to rotate through the rotating rod (44).

2. The magnetic cleaning and separation device of a single-molecule immunoassay detector according to claim 1, wherein: Magnet sheets (22) are embedded and installed on one side of the top of the base (1) and on one side surface of the column (2), and the two magnet sheets (22) are connected together.

3. The magnetic cleaning and separation device of a single-molecule immunoassay detector according to claim 2, wherein: The electric push rod (21) is rotatably connected to the top of the column (2), and a circular hole is formed in the top of the rotating plate (3) and sleeved on the outer ring surface of the electric push rod (21). The liquid transfer and mixing mechanism (4) includes a support frame (41), and the support frame (41) is installed on the top of the electric push rod (21).

4. The magnetic cleaning and separation device of a single-molecule immunoassay detector according to claim 1, wherein: Through holes (31) are symmetrically formed at both ends of the rotating plate (3). The top of the centrifuge tube (32) is provided with an edge baffle with a size larger than the through hole (31). After the centrifuge tube (32) is inserted into the through hole (31), its bottom just touches the top of the base (1). When the rotating plate (3) rotates 180° on the column (2), the positions of the two centrifuge tubes (32) can be alternated.

5. The magnetic cleaning and separation device of a single-molecule immunoassay detector according to claim 3, wherein: The top of the support frame (41) is a right-angled baffle. The pipette (43) and the motor (42) are respectively installed at both ends of the baffle. The pipette (43) is clamped on the support frame (41) and the liquid suction tube is located below the baffle.

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