Auxiliary bracket for water bath of elisa plate

Abstract

The utility model discloses a water bath auxiliary bracket for an elisa plate. The water bath auxiliary bracket comprises an upper frame with a rectangular structure and a bracket body which is formed by four groups of supporting legs uniformly distributed at four corners of the upper frame, a supporting plate is horizontally erected in the upper frame, an adjusting screw rod is vertically screwed and penetrates through the supporting plate, a holding head is arranged at the upper end of the adjusting screw rod, and a supporting basket with a front opening is rotationally connected to the lower end of the adjusting screw rod through a bearing seat; the middle part of the bottom wall of the supporting basket is concave downwards to form an elisa plate clamping groove with a rectangular structure, and the top wall of the supporting basket is fixedly connected with a positioning guide rod which vertically and upwards penetrates through the supporting plate. The ELISA plate fixing device has the advantages that the ELISA plate fixing device can be easily supported and fixed in the water bath kettle, so that the lower half part of the ELISA plate can be horizontally and stably immersed in liquid in the water bath kettle, the ELISA plate is prevented from shaking, the liquid in the water bath kettle is prevented from submerging the upper surface of the ELISA plate, and a sample in a plate hole of the ELISA plate is ensured not to be polluted; the heating rate of a sample water bath on the elisa plate is ensured, the incubation effect is improved, and the success rate of a test and the accuracy of a test result are ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of enzyme-linked immunosorbent assay, in particular to a water bath auxiliary bracket for an enzyme-labeled plate. Background Art

[0002] Enzyme-linked immunosorbent assay (ELISA) is a detection technique that uses enzyme-labeled antigen-antibody samples to specifically bind to known antigens or antibodies adsorbed on the surface of a solid-phase carrier (enzyme-labeled plate), then washes away the free components in the liquid sample, and then qualitatively or quantitatively analyzes the target by measuring the absorbance (OD value) of the sample. The antigen-antibody binding reaction process in this test needs to be carried out at a specific temperature. Its test steps mainly include: adding samples → adding enzymes → incubating → washing → adding developers → incubating → terminating. Multiple incubations are required during the process, usually in a 37°C water bath, keeping the lower half of the enzyme-labeled plate always immersed in water.

[0003] Currently, most often the enzyme-labeled plate is directly placed in the water bath so that it floats on the water surface. This method results in an insufficient depth of the enzyme-labeled plate immersed in water, a slow heating rate of the sample in the water bath, and poor incubation effects. In addition, there are many wells on the enzyme-labeled plate (usually a 96-well enzyme-labeled plate or a 48-well enzyme-labeled plate), and it cannot be guaranteed that all wells have samples added, nor can it be guaranteed that the sample addition amounts in all wells are the same. Therefore, it is difficult to ensure the level of the enzyme-labeled plate floating on the water surface. At this time, once the water surface shakes or the enzyme-labeled plate shakes due to sampling, it is very easy for the liquid in the water bath to overflow the upper surface of the enzyme-labeled plate, resulting in contamination of the samples in the wells, thus causing the entire test to fail or the results to be inaccurate. Summary of the Invention

[0004] The purpose of the utility model is to provide a water bath auxiliary bracket for an enzyme-labeled plate aiming at the defects of the prior art.

[0005] To achieve the above purpose, the utility model can adopt the following technical solutions:

[0006] The water bath auxiliary bracket for an enzyme-labeled plate described in the utility model includes a bracket body composed of an upper frame with a rectangular structure and four groups of legs evenly distributed at the four corners of the upper frame; a support plate is horizontally installed in the upper frame, and an adjusting screw rod is vertically screwed through the support plate. The upper end of the adjusting screw rod is provided with a holding head, and its lower end is rotatably connected to a support basket with a front opening through a bearing seat; the middle part of the bottom wall of the support basket is recessed to form an enzyme-labeled plate card slot with a rectangular structure, and a positioning and guiding rod vertically upwardly passing through the support plate is fixedly connected to the top wall of the support basket.

[0007] Furthermore, the support plate is in a cross-shaped structure, which can minimize the weight while ensuring stability. At this time, the adjusting screw rod is vertically screwed through the center of the support plate, and there are two positioning guide rods, symmetrically arranged on the left and right sides of the adjusting screw rod, to improve the stability when the adjusting screw rod drives the support basket to move up and down.

[0008] Furthermore, the outer surface of the holding head is a non-slip rough surface to increase the friction when grasping and rotating the holding head.

[0009] Furthermore, a bowl-shaped suction cup with an opening facing down is provided at the lower end of each leg. The bowl-shaped suction cup can be tightly adsorbed on the inner wall of the water bath pot, thereby improving the supporting stability of the entire bracket body.

[0010] The advantages of the present utility model are that it can be easily fixed in the water bath pot, and the height of the lower support basket can be adjusted by screwing the adjusting screw rod, so as to adjust the height of the microplate fixed in the support basket, so that the lower half of the microplate can be horizontally and stably immersed in the liquid in the water bath pot, preventing the microplate from shaking, avoiding the liquid in the water bath from overflowing the upper surface of the microplate, ensuring that the samples in the microplate wells are not contaminated, ensuring the heating rate of the samples on the microplate during water bath, improving the incubation effect, and ensuring the success rate of the experiment and the accuracy of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 is a schematic structural view of the present utility model. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0012] Hereinafter, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0013] As Figure 1 described, the microplate water bath auxiliary bracket of the present utility model includes a bracket body 1 for being fixed in a water bath pot. The bracket body is composed of an upper frame 1.1 in a rectangular structure and four groups of legs 1.2 evenly distributed at the four corners of the upper frame; to make the bracket body 1 more stably fixed in the water bath pot, a bowl-shaped suction cup 2 with an opening facing down can also be provided at the lower end of each leg 1.2. The bowl-shaped suction cup 2 can be tightly adsorbed on the bottom surface of the water bath pot, thereby improving the supporting stability of the entire bracket body 1.

[0014] A support plate 3 is horizontally installed in the upper frame 1.1. The support plate 3 is in a cross-shaped structure, which can minimize the weight while ensuring stability.

[0015] A adjusting screw rod 4 is arranged on a support plate 3, and the adjusting screw rod 4 vertically penetrates through the center of the support plate 3 in a screwed manner; a gripping head 5 with an anti-slip rough surface on the outer surface is arranged at the upper end of the adjusting screw rod 4 to increase the friction force when rotating the gripping head 5 for gripping operation. The lower end of the adjusting screw rod 4 is rotatably connected with a support basket 7 with a front opening through a bearing seat 6. The middle part of the bottom wall of the support basket 7 is concave to form an ELISA plate card slot 8 in a rectangular structure. The ELISA plate to be water-bathed can be stably placed in the ELISA plate card slot 8, so that the ELISA plate can move up and down smoothly together with the support basket 7.

[0016] In addition, a positioning and guiding rod 9 vertically upward passing through the support plate 3 is fixedly connected to the top wall of the support basket 7. Preferably, two positioning and guiding rods 9 are arranged, and the two positioning and guiding rods 9 are symmetrically arranged on the left and right sides of the adjusting screw rod 4. The support basket 7 is limited in rotation upward through the positioning and guiding rod 9 to prevent the support basket 7 from rotating randomly or rotating together with the adjusting screw rod 4, and improve the stability when the adjusting screw rod 4 drives the support basket 7 to move up and down.

[0017] During use, the ELISA plate to be water-bathed is stably placed in the ELISA plate card slot 8 in the support basket 7, and the entire bracket body 1 is fixed in a water bath pot; then, the adjusting screw rod 4 is adjusted according to the liquid level position of the liquid in the water bath pot, so that the lower half of the ELISA plate in the support basket 7 can be horizontally and stably immersed in the liquid of the water bath pot; then, the water-bathing operation of the ELISA plate can be carried out, preventing the ELISA plate from shaking, avoiding the liquid in the water bath pot from exceeding the upper surface of the ELISA plate, ensuring that the samples in the ELISA plate holes are not contaminated, ensuring the heating rate of the samples on the ELISA plate during water bath, improving the incubation effect, and ensuring the success rate of the test and the accuracy of the test results.

[0018] It should be noted that all the directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relative position relationship and movement conditions between components in a specific posture (as shown in the drawings). If the specific posture changes, the directional indications will also change accordingly.

Claims

1. An ELISA plate water bath auxiliary support, comprising a support body composed of an upper frame with a rectangular structure and four groups of legs evenly distributed at the four corners of the upper frame; characterized in that: A support plate is horizontally installed within the upper frame. An adjusting screw rod is vertically screwed through the support plate. A gripping head is provided at the upper end of the adjusting screw rod, and the lower end thereof is rotatably connected to a support basket with a front opening through a bearing seat. The middle of the bottom wall of the support basket is recessed to form an ELISA plate card slot with a rectangular structure. A positioning and guiding rod vertically extending upward through the support plate is fixedly connected to the top wall of the support frame.

2. The enzyme-labeled plate water bath auxiliary bracket according to claim 1, wherein: The support plate is in a cross-shaped structure. The adjusting screw rod is vertically screwed through the center of the support plate. There are two positioning and guiding rods, symmetrically arranged on the left and right sides of the adjusting screw rod.

3. The ELISA plate water bath auxiliary bracket according to claim 1, wherein: The outer surface of the gripping head is an anti-slip rough surface.

4. The enzyme-labeled plate water bath auxiliary bracket according to claim 1, wherein: A bowl-shaped suction cup with a downward opening is provided at the lower end of each leg.

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