Microfluidic device
By designing a microfluidic device that includes a desktop test box and a microfluidic device, using the power supply of artificially controlled heaters and temperature sensors, the problem of existing equipment requiring additional manpower and material resources to deal with the test results is solved, simplifying the operation process and reducing costs.
Patent Information
- Application Number
- CN202422466914.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-12
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2034-10-12
AI Technical Summary
Existing microfluidic devices require additional manpower and material resources to handle the test results, which are complex and costly.
A microfluidic device is designed, including a desktop test box and a fixedly connected microfluidic device. The device includes a substrate, a microfluidic chip, a film electrode, a heater, a temperature sensor, a printed circuit board, a microcontroller and a display screen. By artificially controlling the connection between the spring connector and the heater and the temperature sensor, the heater and the temperature sensor are provided with power or power supply to the heater and the temperature sensor, and the operation process is simplified.
It enables display and control of detection results without additional personnel, reducing operational complexity and cost.
Smart Images
Figure CN223170942U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of microfluidics, and particularly relates to a microfluidic device. Background Art
[0002] Microfluidic technology is a powerful tool for diagnosing cancer and infectious diseases because it can integrate different biological processes into a microdevice for various applications. These microdevices can efficiently and accurately process small volumes of solution and reduce the risk of contamination. In addition, they are cost-effective, easy to manufacture on a large scale, and may not require qualified personnel like molecular diagnostic technologies, thus helping to reduce costs. Moreover, these devices integrate various laboratory functions on a single chip and have significant advantages in terms of speed and portability compared to traditional diagnostic methods.
[0003] Microfluidic devices have significantly promoted the field of molecular diagnostics, especially in the detection of cancer using DNA and RNA sequence analysis. Importantly, some microfluidic platforms can detect genetic material without amplification techniques such as PCR, thus reducing complexity and accelerating the diagnostic speed. For example, the Gene-Z device uses a smartphone-based platform for electrochemical detection of DNA sequences (Stedtfeld et al., 2012). It is designed for point-of-care diagnosis and can quickly and sensitively detect pathogens such as HIV and tuberculosis without nucleic acid amplification. Another microfluidic device method is that the VerePLEX biosystem of Veredus Laboratories combines a microarray-based chip with fluorescence detection to simultaneously identify multiple pathogens (Andrea et al., 2015). It can detect specific DNA / RNA sequences from clinical samples without amplification, such as respiratory viruses. Meanwhile, Affymetrix's gene chip system performs nucleic acid hybridization on a microarray chip to detect specific DNA / RNA sequences (Karsten et al., 2008). It is widely used in research and clinical diagnosis to detect gene mutations and pathogens. Similarly, the Nanosphere Verigene system uses gold nanoparticle probes for hybridization-based detection. In the field of surface plasmon resonance (SPR)-based detection, General Electric Healthcare has developed the Biacore T200, and Texas Instruments has developed the Spreeta SPR sensor. (Wear et al., 2017; Brenda et al., 2001). The Biacore T200 is an SPR-based system that can detect the interaction between biomolecules in real time and detect pathogens by monitoring the binding of DNA / RNA sequences to complementary probes on the sensor surface. The Spreeta SPR sensor uses SPR technology for label-free detection of DNA / RNA hybridization. These sensors can be integrated into microfluidic devices for real-time monitoring of genetic material. In addition, the LumiraDx platform also provides a portable microfluidic platform that uses fluorescence-based lateral flow immunoassay for rapid detection of infectious diseases including COVID-19. The device can provide rapid test results and is suitable for point-of-care testing (Tait et al., 2019). Devices that do not require genetic material amplification have obvious advantages such as reduced complexity, reduced cost, and faster results. However, all of the above devices require additional equipment to display the results of processed samples and sometimes additional personnel to complete the analysis process, thus increasing complexity and cost. Summary of the Invention
[0004] The purpose of the present invention is to provide a microfluidic device to solve the problems in the prior art that additional human and material resources are required to obtain test results, and the complexity and cost are relatively high.
[0005] To achieve the above object, the present utility model provides the following technical solutions: a microfluidic device, including a desktop test box and a microfluidic device fixedly connected to the desktop test box. The microfluidic device includes a substrate and a microfluidic chip, the microfluidic chip is fixedly connected to the substrate, a thin-film electrode is arranged on the substrate, a heater and a temperature sensor are arranged on the thin-film electrode, a printed circuit board is arranged on the desktop test box, and the printed circuit board is movably connected to the heater and the temperature sensor.
[0006] Preferably, a spring-type connector is fixedly arranged on the printed circuit board, a rotating shaft for driving one end of the printed circuit board to lift upward is rotatably installed on the desktop test box, and the rotating shaft is movably connected to the printed circuit board.
[0007] Preferably, a sample chamber and a mixing chamber are arranged on the microfluidic chip, a microchannel is arranged between the sample chamber and the mixing chamber, the microchannel penetrates through the sample chamber and the mixing chamber, a peristaltic pump is fixedly installed on the desktop test box, and one end of the microchannel is fixedly connected to the peristaltic pump.
[0008] Preferably, a lateral flow strip is fixedly installed on the substrate, and the lateral flow strip is located on one side close to the microchannel.
[0009] Preferably, a silicon nitride passivation layer is arranged between the microfluidic chip and the thin-film electrode.
[0010] Preferably, a laser is arranged in the mixing chamber.
[0011] Preferably, a display screen is arranged on the desktop test box, a microcontroller is arranged on the microfluidic device, and the display screen is electrically connected to the microcontroller.
[0012] The technical effects and advantages of the present utility model: During the test, the liquid is detected by the microfluidic device, connected to the display screen on the desktop test box through the microcontroller to display data, and the connection relationship between the spring-type connector and the heater and the temperature sensor is controlled by manual cooperation to supply power or stop supplying power to the heater and the temperature sensor, achieving the purpose of convenient use. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 It is a schematic structural diagram of the microfluidic device of the present utility model;
[0014] Figure 2 It is a schematic installation structure diagram of the microfluidic device of the present utility model;
[0015] Figure 3Schematic diagram of the spring-type connector structure of the present utility model.
[0016] In the figure: 1. Microfluidic device; 11. Substrate; 12. Microfluidic chip; 13. Silicon nitride passivation layer; 14. Sample chamber; 15. Mixing chamber; 16. Microchannel; 2. Desktop test box; 21. Peristaltic pump; 3. Printed circuit board; 4. Display screen; 5. Spring-type connector; 6. Rotating shaft. Specific embodiments
[0017] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.
[0018] The present utility model provides a microfluidic device as shown in the figure, including a desktop test box 2 and a microfluidic device 1 fixedly connected to the desktop test box 2. The microfluidic device 1 includes a substrate 11 and a microfluidic chip 12. The microfluidic chip 12 is fixedly connected to the substrate 11. A thin-film electrode is provided on the substrate 11, and a heater and a temperature sensor are provided on the thin-film electrode. A printed circuit board 3 is provided on the desktop test box 2, and the printed circuit board 3 is movably connected to the heater and the temperature sensor. A silicon nitride passivation layer 13 is provided between the microfluidic chip 12 and the thin-film electrode. The microfluidic device 1 is composed of a combination of the substrate 11, the thin-film electrode, and the microfluidic chip 12. The thin-film electrode is drawn on the outer wall of the upper side of the substrate 11. The microfluidic chip 12 covers the base, and a silicon nitride passivation layer 13 is provided between the microfluidic chip 12 and the thin-film electrode to achieve electrical isolation between the electrode and the sample and reagent. A microcontroller is provided on the microfluidic device 1 to control the microchannel 16 and the microfluidic chip 12. The microcontroller is connected to the display screen 4 on the desktop test box 2 and connected to the power supply to display the detection data. A printed circuit board 3 for controlling the start and stop of the heater and the temperature sensor on the thin-film electrode is provided on the desktop test box 2. During the test, the connection relationship between the spring-type connector 5 and the heater and the temperature sensor can be manually controlled to supply power or stop supplying power to the heater and the temperature sensor.
[0019] Specifically, a spring-type connector 5 is fixedly provided on the printed circuit board 3, and a rotating shaft 6 for driving one end of the printed circuit board 3 to lift upward is rotatably installed on the desktop test box 2. The rotating shaft 6 is movably connected to the printed circuit board 3. Refer to the attached Figure 3As shown, the printed circuit board 3 is connected to a power source. When controlling the spring-loaded connector 5, the rotating shaft 6 can be manually rotated. A protruding connecting block is provided on the outer wall of the rotating shaft 6. By changing the position of the connecting block, it contacts the spring-loaded connector 5 provided on the printed circuit board 3 and moves upward. The spring-loaded connector 5 is electrically contacted with the heater, driving the heater to start, thereby heating the microfluidic device 1.
[0020] Specifically, a sample chamber 14 and a mixing chamber 15 are provided on the microfluidic chip 12, a microchannel 16 is provided between the sample chamber 14 and the mixing chamber 15, and the microchannel 16 runs through the sample chamber 14 and the mixing chamber 15. A peristaltic pump 21 is fixedly installed on the desktop test box 2, and one end of the microchannel 16 is fixedly connected to the peristaltic pump 21. A transverse flow belt is fixedly installed on the base 11, and the transverse flow belt is located on the side close to the microchannel 16. A laser is provided in the mixing chamber 15. A display screen 4 is provided on the desktop test box 2, and a microcontroller is provided on the microfluidic device 1. The display screen 4 is electrically connected to the microcontroller. Figure 1 As shown, a sample chamber 14 and a mixing chamber 15 are provided on the microfluidic chip 12 and are connected by a microchannel 16. When conducting a test, a peristaltic pump 21 delivers the sample and reagent from the microchannel 16 into the sample chamber 14, and the sample and reagent are mixed through the microchannel 16 portion between the sample chamber 14 and the mixing chamber 15, and finally enter the mixing chamber 15. A laser is provided in the mixing chamber 15 for irradiating the mixed reagent. After irradiation, the sample and reagent gradually flow from the mixing chamber 15 to the other end of the microchannel 16 under the action of the peristaltic pump 21. The other end of the microchannel 16 is connected to the base, and a transverse flow belt is provided on the base to adsorb the mixed liquid through the transverse flow belt.
[0021] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A microfluidic device, characterized in that: It includes a desktop test chamber (2) and a microfluidic device (1) fixedly connected to the desktop test chamber (2). The microfluidic device (1) includes a substrate (11) and a microfluidic chip (12). The microfluidic chip (12) is fixedly connected to the substrate (11). A thin-film electrode is provided on the substrate (11), and a heater and a temperature sensor are provided on the thin-film electrode. A printed circuit board (3) is provided on the desktop test chamber (2), and the printed circuit board (3) is movably connected to the heater and the temperature sensor.
2. The microfluidic device according to claim 1, characterized in that: A spring-type connector (5) is fixedly provided on the printed circuit board (3). A rotating shaft (6) for driving one end of the printed circuit board (3) to lift upward is rotatably installed on the desktop test chamber (2), and the rotating shaft (6) is movably connected to the printed circuit board (3).
3. The microfluidic device according to claim 2, characterized in that: A sample chamber (14) and a mixing chamber (15) are provided on the microfluidic chip (12). A microchannel (16) is provided between the sample chamber (14) and the mixing chamber (15). The microchannel (16) penetrates through the sample chamber (14) and the mixing chamber (15). A peristaltic pump (21) is fixedly installed on the desktop test chamber (2), and one end of the microchannel (16) is fixedly connected to the peristaltic pump (21).
4. The microfluidic device according to claim 3, characterized in that: A lateral flow strip is fixedly installed on the substrate (11), and the lateral flow strip is located on one side close to the microchannel (16).
5. The microfluidic device according to claim 4, wherein: A silicon nitride passivation layer (13) is provided between the microfluidic chip (12) and the thin-film electrode.
6. The microfluidic device according to claim 5, characterized in that: A laser is provided in the mixing chamber (15).
7. The microfluidic device according to claim 6, characterized in that: A display screen (4) is provided on the desktop test chamber (2). A microcontroller is provided on the microfluidic device (1), and the display screen (4) is electrically connected to the microcontroller.