Culture device for cell separation

Abstract

The utility model relates to the technical field of biological experiments, and discloses a culture device for cell separation, which comprises a culture bin, a connecting frame and a plurality of connecting rods, foot stools are fixedly connected to four sides of the bottom end of the culture bin, and a rotating plate is connected to the bottom end of the inner wall of the culture bin through a centrifugal group and used for rotating the rotating plate. The top ends of the connecting frames are rotationally connected with deflection tables, the outer walls of the deflection tables are connected with gear rods through conveying sets so that the gear rods and the deflection tables can synchronously rotate, the inner walls of the top ends of the deflection tables are rotationally connected with rotating rods, and the outer walls of the top ends of the rotating rods are connected with arc groove rings through deflection sets so that the rotating rods can deflect along the arc groove rings. According to the device disclosed by the utility model, the centrifugal force effect of the device on a solution in a test tube is improved under the action of the deflection motion of the rotating rod at the deflection table and the overall circular motion of the rotating plate, and the contraction force of the clamping claws is changed by the displacement of the adjusting ring, so that the test tubes with different diameters can be conveniently clamped.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to a culture device for cell separation. Background Art

[0002] A culture device for cell separation is a device used to separate and culture cells from samples, and is usually used in fields such as biomedical research, cell therapy, and tissue engineering. The core function of such a device is to provide a closed space that can precisely control environmental conditions to support the survival and proliferation of cells, and different types of cells can be separated by centrifugal force.

[0003] After retrieval, a suspension separation device for macrophage culture with the publication number CN218321381U belongs to the technical field of cell culture media. The suspension separation device for macrophage culture includes a driving component and a separation component. The driving component includes a box body, a partition plate, a rotating disk, and a motor. The partition plate is arranged inside the box body. The separation component includes an outer cylinder, an inner cylinder, a filter screen, and a cylinder cover. Between the outer cylinder and the inner cylinder, several slots are opened at the top of the rotating disk, and the outer cylinder is inserted into the slots. By setting the box body, the partition plate, the rotating disk, and the motor, it is used to drive the separation component to rotate for centrifugal separation. By setting the outer cylinder, the inner cylinder, the filter screen, and the cylinder cover, the cell suspension can be separated into each outer cylinder and inner cylinder for separate centrifugal separation. In this way, when taking it, the shaking amplitude of the liquid is small, which largely avoids remixing. At the same time, the independently arranged cylinders are convenient for cleaning and reuse, and are also convenient for taking.

[0004] Based on the above patent, by setting the box body, the partition plate, the rotating disk, and the motor, it is used to drive the separation component to rotate for centrifugal separation. By setting the outer cylinder, the inner cylinder, the filter screen, and the cylinder cover, the cell suspension can be separated into each outer cylinder and inner cylinder for separate centrifugal separation. In this way, when taking it, the shaking amplitude of the liquid is small, which largely avoids remixing. At the same time, the independently arranged cylinders are convenient for cleaning and reuse, and are also convenient for taking. However, when shaking the solution in the test tube in this patent, the centrifugal force effect is not good, and the insufficient centrifugal force causes the cells not to be fully separated during centrifugation, resulting in the target cells being mixed with other cells or impurities, affecting the accuracy and purity of the separation result. Summary of the Utility Model

[0005] The purpose of the utility model is to solve the disadvantages existing in the prior art, and to propose a culture device for cell separation, which improves the centrifugal force effect of the device on the solution in the test tube and is convenient for clamping test tubes with different diameters.

[0006] To achieve the above purpose, the utility model provides the following technical solutions:

[0007] A culture device for cell separation, comprising:

[0008] A culture chamber, with feet fixedly connected to four sides of the bottom end of the culture chamber. The bottom end inner wall of the culture chamber is connected to a rotating plate through a centrifugal group for the rotating plate to rotate.

[0009] A connecting frame, with deflection platforms rotatably connected to the top ends of the connecting frame. The outer wall of the deflection platform is connected to a gear rod through a transmission group for the gear rod to rotate synchronously with the deflection platform. The inner wall of the top end of the deflection platform is rotatably connected to a rotating rod, and the outer wall of the top end of the rotating rod is connected to an arc groove ring through a deflection group for the rotating rod to deflect along the arc groove ring.

[0010] A fixing frame, with a threaded ring fixedly connected to the top end of the fixing frame. The top end of the threaded ring is connected to an adapter ring through a rotating group for the adapter ring to move. The outer wall of the top end of the adapter ring is rotatably connected to four sides of a shrinkage plate, and the top end of the shrinkage plate is connected to a clamping claw through a clamping group for the clamping claw to clamp a test tube.

[0011] Further, the centrifugal group includes a driving motor fixedly connected to the inner wall of the bottom end of the culture chamber, and the driving end of the driving motor is fixedly connected to the outer wall of the bottom end of the rotating plate.

[0012] Further, a sealing cover is rotatably connected to the outer wall of the top end of the culture chamber, and the bottom ends of the connecting frames are respectively fixedly connected to four sides of the outer wall of the top end of the rotating plate.

[0013] Further, the transmission group includes transmission wheels fixedly connected to the outer walls of the bottom ends of the gear rod and the deflection platform, and the transmission wheels are connected by a conveyor belt.

[0014] Further, transmission gears are fixedly connected to the bottom ends of the deflection platforms, a gear ring is fixedly connected to the inner wall of the bottom end of the culture chamber, and the gear ring and the transmission gears are meshed.

[0015] Further, the deflection group includes spherical gaskets fixedly connected to both the upper and lower ends of the rod body of the rotating rod, gear pieces are fixedly connected to the outer wall of the rotating rod corresponding to the opposite ends of the spherical gaskets, and the gear pieces and the gear rod are meshed.

[0016] Further, the rotating group includes adjusting rings threadedly connected to the outer walls of the threaded rings, the bottom end of the adapter ring is rotatably connected to the top end of the adjusting ring, and fixing plates are fixedly connected to four sides of the outer wall of the fixing frame.

[0017] Further, the clamping group includes rotating blocks rotatably connected to opposite ends of the shrinkage plate, the opposite ends of the rotating blocks are rotatably connected to the inner walls of the opposite ends of the fixing plates, traction plates are rotatably connected to the top ends of the rotating blocks, and the bottom ends of the clamping claws are rotatably connected to the top ends of the traction plates.

[0018] The utility model has the following beneficial effects:

[0019] 1. In the utility model, under the action of the deflection movement of the rotating rod at the deflection table and the overall circular movement of the rotating plate, the centrifugal force effect of the device on the solution in the test tube is improved, so that cells and other particulate matters are better stratified according to their density differences, thereby improving the separation effect, and the target cells can be separated from other cells or impurities more effectively, and the separation purity is improved.

[0020] 2. In the utility model, by changing the contraction force of the clamping claws through the displacement of the adjusting ring, it is convenient to clamp test tubes with different diameters, so that the device can adapt to more types and sizes of experimental requirements, can easily meet various experimental requirements, and improve the versatility of the equipment, reduce the diverse requirements for equipment in the laboratory, meet personalized experimental needs, and make the experimental design more flexible. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a three-dimensional view of a cell separation culture device proposed by the utility model;

[0022] Figure 2 is a half-sectional view of the culture chamber of a cell separation culture device proposed by the utility model;

[0023] Figure 3 is a half-sectional view of the rotating plate of a cell separation culture device proposed by the utility model;

[0024] Figure 4 is a half-sectional view of the connecting frame of a cell separation culture device proposed by the utility model;

[0025] Figure 5 is a schematic structural view of the fixing frame of a cell separation culture device proposed by the utility model;

[0026] Figure 6 is a half-sectional view of the adjusting ring of a cell separation culture device proposed by the utility model.

[0027] LEGEND DESCRIPTION:

[0028] 1. Culture chamber; 2. Leg frame; 3. Sealing cover; 4. Driving motor; 5. Gear ring; 6. Rotating plate; 7. Connecting frame; 8. Deflection table; 9. Rotating rod; 10. Arc groove ring; 11. Fixing frame; 12. Threaded ring; 13. Fixed plate; 14. Clamping claw; 15. Transmission wheel; 16. Transmission belt; 17. Gear rod; 18. Transmission gear; 19. Spherical gasket; 20. Gear piece; 21. Adjusting ring; 22. Adapter ring; 23. Traction plate; 24. Shrinkage plate; 25. Rotating block. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present utility model.

[0030] Referring to Figure 1 and Figure 2 , an embodiment provided by the present utility model: a culture device for cell separation, including a culture chamber 1, four sides of the bottom end of the culture chamber 1 are fixedly connected with feet 2, a driving motor 4 is fixedly connected to the inner bottom wall of the culture chamber 1, the driving end of the driving motor 4 is fixedly connected to the outer bottom wall of the rotating plate 6, a sealing cover 3 is rotatably connected to the outer wall of the top end of the culture chamber 1, and the bottom ends of the connecting frames 7 are respectively fixedly connected to the outer walls of the four sides of the top end of the rotating plate 6.

[0031] Specifically: when the inner driving motor 4 of the culture chamber 1 is started, the motor 4 drives the transmission gear 18 connected thereto to rotate along the gear ring 5 through the gear ring 5 driven by it. This rotation enables the movement of the gear ring 5 to be transmitted, thereby driving the operation of related mechanical devices. At the same time, the feet 2 play an important role in this process. It can effectively buffer the vibration generated by the centrifugal force and ensure the stability of the culture chamber 1. When the sealing cover 3 is covered on the opening of the culture chamber 1, it can ensure the tightness inside the culture chamber 1, thereby maintaining the closed state of its internal environment. The closed environment is beneficial to generating conditions such as temperature and humidity during the culture process.

[0032] Referring to Figure 3 and Figure 4 , the connecting frame 7, deflection platforms 8 are rotatably connected to the top ends of the connecting frame 7, conveyor wheels 15 are fixedly connected to the outer bottom walls of the gear rods 17 and the deflection platforms 8, the conveyor wheels 15 are connected by a conveyor belt 16, rotating rods 9 are rotatably connected to the inner walls of the top ends of the deflection platforms 8, transmission gears 18 are fixedly connected to the bottom ends of the deflection platforms 8, a gear ring 5 is fixedly connected to the inner bottom wall of the culture chamber 1, the gear ring 5 and the transmission gear 18 are meshed, spherical gaskets 19 are fixedly connected to both the upper and lower ends of the rod body of the rotating rod 9, and gear pieces 20 are fixedly connected to the outer wall of the rotating rod 9 corresponding to the opposite ends of the spherical gaskets 19, and the gear pieces 20 and the gear rods 17 are meshed.

[0033] Specifically: When the transmission gear 18 starts to rotate, it drives the deflection table 8 to rotate. The rotation of the deflection table 8 transmits the motion to the gear rod 17 through the connected conveyor wheels 15 and conveyor belt 16, causing the gear rod 17 to rotate accordingly. At the same time, the rotation of the deflection table 8 makes the spherical gasket 19 on the rotating rod 9 move along an arc trajectory within the arc groove ring 10. During the synchronous rotation of the gear rod 17, it deflects through the gear piece 20, thereby driving the rotating rod 9. The movement of the rotating rod 9 causes the test tube on the fixed frame 11 to deflect. At the same time, the rotation of the rotating plate 6 makes the test tube perform a circular motion. With the combination of deflection and circular rotation, the test tube can generate a higher centrifugal force, which is of great significance for the material mixing and separation process in the culture chamber 1. Through this comprehensive movement, the test tube can obtain a more uniform centrifugal force effect within the culture chamber 1, thereby improving the culture effect and experimental accuracy.

[0034] Refer to Figure 5 and Figure 6 , the fixed frame 11, a threaded ring 12 is fixedly connected to the top of the fixed frame 11, an adjusting ring 21 is threadedly connected to the outer wall of the threaded ring 12, the bottom end of the adapter ring 22 is rotatably connected to the top end of the adjusting ring 21, fixing plates 13 are fixedly connected to the four sides of the outer wall of the fixed frame 11, retractable plates 24 are rotatably connected to the four sides of the outer wall of the top end of the adapter ring 22, rotating blocks 25 are rotatably connected to the opposite ends of the retractable plates 24, the opposite ends of the rotating blocks 25 are rotatably connected to the inner walls of the opposite ends of the fixing plates 13, traction plates 23 are rotatably connected to the top ends of the rotating blocks 25, and the bottom ends of the clamping claws 14 are rotatably connected to the top ends of the traction plates 23.

[0035] Specifically: When clamping a test tube filled with solution, first rotate the adjusting ring 21 to make it move along the threaded ring 12. The rotation action of the adjusting ring 21 is transmitted through the adapter ring 22, further driving the movement of the retractable plate 24. The movement of the retractable plate 24 causes the rotating block 25 to contract, so that the traction plate 23 also moves accordingly. The movement of the traction plate 23 makes the clamping claws 14 clamp the test tube. The rotation amplitude of the adjusting ring 21 directly affects the clamping force of the clamping claws 14. By rotating the adjusting ring 21, the opening and closing degree of the clamping claws 14 can be precisely controlled. This adjustment function enables the clamping claws 14 to adapt to test tubes of different diameters. Regardless of the size of the test tube, the clamping force of the clamping claws 14 can be adjusted to achieve a stable clamping, thereby ensuring the safety and stability of the test tube during the operation process.

[0036] Working principle: When the drive motor 4 in the culture chamber 1 starts, the drive motor 4 drives the gear ring 5 to make the transmission gear 18 connected thereto rotate along the gear ring 5. The tripod 2 can buffer the vibration caused by the centrifugal force generated in the culture chamber 1. When the sealing cover 3 is covered on the culture chamber 1, the culture chamber 1 can be kept airtight. When the transmission gear 18 rotates, the transmission gear 18 drives the deflection table 8 to rotate. When the deflection table 8 rotates, the conveyor wheel 15 connected thereto drives the gear rod 17 to rotate through the conveyor belt 16. When the deflection table 8 rotates, the spherical gasket 19 at the rotating rod 9 rotates along the arc groove ring 10. When the gear rod 17 rotates synchronously, the gear rod 17 deflects through the gear piece 20, so that the rotating rod 9 drives the test tube connected to the fixed frame 11 to deflect and rotate in a circular motion under the rotation of the rotating plate 6. Thus, the test tube rotates in a circular motion while deflecting, thereby increasing the centrifugal force of the test tube in the culture chamber 1. When clamping the test tube filled with solution, rotate the adjusting ring 21 to make it move along the threaded ring 12, so that the adjusting ring 21 drives the contraction plate 24 through the adapter ring 22 to contract the traction plate 23 by the rotating block 25. The traction plate 23 drives the clamping jaw 14 to clamp the test tube, and the adjusting ring 21 adjusts the clamping force of the clamping jaw 14 through the moving amplitude, so as to facilitate clamping test tubes with different diameters.

[0037] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A culture device for cell separation, characterized in that, Comprising: A culture chamber (1), four sides of the bottom end of the culture chamber (1) are fixedly connected with feet (2), and the bottom end inner wall of the culture chamber (1) is connected with a rotating plate (6) through a centrifugal group for the rotating plate (6) to rotate; A connecting frame (7), the top ends of the connecting frame (7) are rotatably connected with deflection platforms (8), the outer wall of the deflection platform (8) is connected with a gear rod (17) through a transmission group for the gear rod (17) to rotate synchronously with the deflection platform (8), the inner walls of the top ends of the deflection platform (8) are rotatably connected with rotating rods (9), and the outer walls of the top ends of the rotating rods (9) are connected with an arc groove ring (10) through a deflection group for the rotating rods (9) to deflect along the arc groove ring (10); A fixing frame (11), the top end of the fixing frame (11) is fixedly connected with a threaded ring (12), the top end of the threaded ring (12) is connected with an adapter ring (22) through a rotating group for the adapter ring (22) to move, four sides of the outer wall of the top end of the adapter ring (22) are rotatably connected with shrinkage plates (24), and the top ends of the shrinkage plates (24) are connected with clamping claws (14) through a clamping group for the clamping claws (14) to clamp a test tube.

2. The culture device for cell separation according to claim 1, characterized in that: The centrifugal group includes a driving motor (4) fixedly connected to the bottom end inner wall of the culture chamber (1), and the driving end of the driving motor (4) is fixedly connected to the outer wall of the bottom end of the rotating plate (6).

3. The cell separation culture device according to claim 1, wherein: The outer wall of the top end of the culture chamber (1) is rotatably connected with a sealing cover (3), and the bottom ends of the connecting frames (7) are respectively fixedly connected to four sides of the outer wall of the top end of the rotating plate (6).

4. The culture device for cell separation according to claim 1, wherein: The transmission group includes transmission wheels (15) fixedly connected to the outer walls of the bottom ends of the gear rod (17) and the deflection platform (8), and the transmission wheels (15) are connected through a conveyor belt (16).

5. The culture device for cell separation according to claim 1, characterized in that: The bottom ends of the deflection platforms (8) are fixedly connected with transmission gears (18), the bottom end inner wall of the culture chamber (1) is fixedly connected with a gear ring (5), and the gear ring (5) and the transmission gears (18) are meshed with each other.

6. The culture device for cell separation according to claim 1, characterized in that: The deflection group includes spherical gaskets (19) fixedly connected to the upper and lower ends of the rod body of the rotating rod (9), gear pieces (20) are fixedly connected to the outer wall of the rotating rod (9) corresponding to the opposite ends of the spherical gaskets (19), and the gear pieces (20) and the gear rod (17) are meshed with each other.

7. A culture device for cell separation according to claim 1, characterized in that: The rotating group includes adjusting rings (21) threadedly connected to the outer walls of the threaded rings (12), the bottom end of the adapter ring (22) is rotatably connected to the top end of the adjusting ring (21), and fixing plates (13) are fixedly connected to four sides of the outer wall of the fixing frame (11).

8. The culture device for cell separation according to claim 1, wherein: The clamping group includes rotating blocks (25) rotatably connected to the opposite ends of the shrinkage plates (24), the opposite ends of the rotating blocks (25) are rotatably connected to the inner walls of the opposite ends of the fixing plates (13), traction plates (23) are rotatably connected to the top ends of the rotating blocks (25), and the bottom ends of the clamping claws (14) are rotatably connected to the top ends of the traction plates (23).

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