Nucleic acid purification device convenient to operate

Abstract

The utility model discloses a nucleic acid purification device convenient to operate, which belongs to the technical field of nucleic acid extraction and purification and comprises a nucleic acid purification device shell, and a stirring component convenient for stirring and mixing and convenient to disassemble and replace is mounted on the nucleic acid purification device shell; the stirring assembly convenient to disassemble and replace comprises a driving device and a stirring device convenient to disassemble, the nucleic acid purification device shell is connected with the driving device, and the driving device is connected with the stirring device convenient to disassemble; a waste liquid collecting assembly for preventing waste liquid from dripping is mounted on the stirring assembly convenient to disassemble and replace; a fixing assembly for fixing and clamping is mounted on the nucleic acid purification device shell; and a door assembly for isolating and sealing is mounted on the nucleic acid purification device shell. Through the mode, the magnetic bead rod is quickly replaced, so that the problem that the purification effect is reduced due to the reduction of the adsorption capacity of the magnetic beads due to the reduction of the magnetism after the magnetic bead rod is used for a long time is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction and purification, and particularly relates to a nucleic acid purification device which is convenient to operate. Background Art

[0002] In the extraction and purification of nucleic acids, magnetic beads that adsorb nucleic acid molecules are usually stirred and adsorbed by a magnetic rod, and then transferred to an eluent for elution, so that purified nucleic acids can be obtained.

[0003] For example, the Chinese utility model patent CN220766961U discloses a nucleic acid extraction and purification device, in which a deep well plate encapsulating reagent set is fixedly arranged on a well plate preheating plate, a magnetic needle sleeve is vertically and fixedly arranged on a magnetic sleeve rack, and a magnetic rod assembly is arranged above the magnetic needle sleeve.

[0004] However, it still has the following drawbacks. When the device finishes extracting nucleic acids and is ready for elution, the upward-moving magnetic rod may carry the liquid during stirring and may drip on the device during the transfer process.

[0005] Based on this, the utility model designs a nucleic acid purification device which is convenient to operate to solve the above problems. Summary of the Utility Model

[0006] In view of the above drawbacks of the prior art, the utility model provides a nucleic acid purification device which is convenient to operate.

[0007] To achieve the above purposes, the utility model is realized through the following technical solutions:

[0008] A nucleic acid purification device which is convenient to operate, including a housing of the nucleic acid purification device, on which a stirring assembly that is convenient for stirring and mixing and is convenient for disassembly and replacement is installed;

[0009] The stirring assembly that is convenient for disassembly and replacement includes a driving device and a stirring device that is convenient for disassembly. The housing of the nucleic acid purification device is connected to the driving device, and the driving device is connected to the stirring device that is convenient for disassembly;

[0010] A waste liquid collection assembly for preventing waste liquid from dripping is installed on the stirring assembly that is convenient for disassembly and replacement;

[0011] A fixing assembly for fixing and clamping is installed on the housing of the nucleic acid purification device;

[0012] A door assembly for isolation and sealing is installed on the housing of the nucleic acid purification device.

[0013] Further, the driving device includes a T-shaped support plate, a second motor, a pulley, a belt, a mounting plate, a support block, and a plastic sleeve. A T-shaped support plate is fixedly connected to the inner top of the nucleic acid purification device housing. The right end of the T-shaped support plate is fixedly connected to the second motor. The output end of the second motor passes through the T-shaped support plate and is fixedly connected to the upper pulley. The left side of the lower end of the T-shaped support plate is rotatably connected to a pulley. A pair of pulleys are connected by a belt drive. The outer wall of the belt is fixedly connected to a support block. The lower end of the support block is fixedly connected to a mounting plate. The lower end of the mounting plate is slidably connected to a plastic sleeve.

[0014] Further, the T-shaped support plate is connected to a stirring device that is easy to disassemble.

[0015] Further, the T-shaped support plate is connected to a waste liquid collection assembly.

[0016] Further, the stirring device that is easy to disassemble includes a cylinder, a fixing plate, a magnetic bead rod, a second clamping block, a second spring, a locking groove, a limiting post, and a T-shaped sliding groove. The front end of the T-shaped support plate is fixedly connected to a cylinder. The output end of the cylinder is fixedly connected to a fixing plate. The magnetic bead rod is slidably connected to the lower end of the fixing plate. T-shaped sliding grooves are opened at both the left and right ends of the fixing plate. A second clamping block is slidably connected to the T-shaped sliding groove. A locking groove is opened at the upper end of the T-shaped sliding groove. A limiting post is fixedly connected to the outer wall of the second clamping block. The limiting post is slidably connected to the locking groove. One end of the second spring is fixedly connected to the inner wall of the larger end of the T-shaped sliding groove, and the other end of the second spring is fixedly connected to the second clamping block.

[0017] Further, the waste liquid collection assembly includes a first motor, a rotating shaft, a baffle, and a sponge. The rear end of the T-shaped support plate is fixedly connected to a first motor. The output end of the first motor passes through the T-shaped support plate and is fixedly connected to a rotating shaft. A baffle is fixedly connected to the outer wall of the rotating shaft. A sponge is fixedly connected to the left end of the baffle.

[0018] Further, the fixing assembly includes a rotary table, a limiting block, a limiting sliding groove, a first clamping block, a sliding block, and a first spring. The rotary table is fixedly connected to the inner bottom of the nucleic acid purification device housing. A plurality of pairs of limiting blocks are fixedly connected to the upper end of the rotary table at equal intervals. A pair of limiting sliding grooves are opened on each limiting block. A pair of sliding blocks are slidably connected to the limiting sliding grooves. The opposite ends of the pair of sliding blocks are fixedly connected to a first clamping block. One end of the first spring is fixedly connected to the inner wall of the limiting sliding groove, and the other end of the first spring is fixedly connected to the first clamping block.

[0019] Further, the door assembly includes a sealing door, a handle, and a hinge. A pair of hinges are fixedly connected to the front end of the nucleic acid purification device housing. The sealing door is rotatably connected to the lower ends of the pair of hinges. The lower side of the front end of the sealing door is fixedly connected to a handle.

[0020] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0021] 1. In the present utility model, the magnetic bead rod of the stirring device with a conveniently detachable stirring component is slid into the fixing plate. At this time, by pressing the second clamping block, the limiting column slides along the locking groove while compressing the second spring to contract. When the second clamping block clamps the magnetic bead rod, the second clamping block is locked by rotating the second clamping block to drive the limiting column to rotate in the locking groove. When the device is ready to stir the material, power is provided by the second motor on the T-shaped support plate of the driving device. The upper pulley is driven to rotate by the output end of the second motor, and the upper pulley drives the lower pulley to rotate through the belt. The support block and the mounting plate are driven to move by the rotation of the belt, and the plastic sleeve is driven to move downward by the movement of the mounting plate and inserted into the reagent kit for stirring. When the device is ready to adsorb the magnetic beads, power is provided by the cylinder. The fixing plate is driven to move downward by the output end of the cylinder, and the magnetic bead rod is driven to move downward by the movement of the fixing plate. The magnetic bead rod moves downward and is inserted into the plastic sleeve to adsorb the magnetic beads in the reagent kit. By quickly replacing the magnetic bead rod, it is avoided that the magnetic adsorption ability of the magnetic bead rod decreases after long-term use, resulting in a decrease in the purification effect.

[0022] 2. In the present utility model, power is provided by the first motor of the waste liquid collection component. The rotating shaft is driven to rotate by the output end of the first motor, the baffle is driven to rotate by the rotation of the rotating shaft, and the sponge is driven to rotate to directly below the plastic sleeve by the rotation of the baffle. The sponge absorbs the liquid dripping when the plastic sleeve moves upward. By absorbing the nucleic acid purification extract, it is avoided that the liquid drips into the device and causes pollution to the device, resulting in possible impurities in the subsequent purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0024] Figure 1 is a three-dimensional view of a nucleic acid purification device that is easy to operate according to the present utility model Figure 1 ;

[0025] Figure 2 is a front view of a nucleic acid purification device that is easy to operate according to the present utility model;

[0026] Figure 3 is a left view of a nucleic acid purification device that is easy to operate according to the present utility model;

[0027] Figure 4 is a sectional view taken along the Figure 3 A-A direction;

[0028] Figure 5 Partial part drawing of the fixing component of a nucleic acid purification device that is easy to operate according to the present utility model;

[0029] Figure 6 Partial part drawing of the waste liquid collection component of a nucleic acid purification device that is easy to operate according to the present utility model;

[0030] Figure 7 Partial part of the stirring component that is easy to disassemble and replace of a nucleic acid purification device that is easy to operate according to the present utility model Figure 1 ;

[0031] Figure 8 Partial part of the stirring component that is easy to disassemble and replace of a nucleic acid purification device that is easy to operate according to the present utility model Figure 2 ;

[0032] Figure 9 is Figure 8 The enlarged view of position B in

[0033] The reference numerals in the figure respectively represent:

[0034] 1. Nucleic acid purification device housing; 2. Door assembly; 21. Sealed door; 22. Handle; 23. Hinge; 3. Fixing component; 31. Rotary table; 32. Limit block; 33. Limit sliding groove; 34. Clamping block 1; 35. Slide block; 36. Spring 1; 4. Waste liquid collection component; 41. Motor 1; 42. Rotating shaft; 43. Baffle; 44. Sponge; 5. Stirring component that is easy to disassemble and replace; 51. Driving device; 52. Stirring device that is easy to disassemble; 511. T-shaped support plate; 512. Motor 2; 513. Pulley; 514. Belt; 515. Mounting plate; 516. Support block; 517. Plastic sleeve; 521. Cylinder; 522. Fixed plate; 523. Magnetic bead rod; 524. Clamping block 2; 525. Spring 2; 526. Locking groove; 527. Limit post; 528. T-shaped sliding groove. Detailed implementation manners

[0035] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0036] The "left", "right", "front", "rear", "upper" and "lower" mentioned in the following description are oriented in the perspective direction of the front view.

[0037] Example 1

[0038] In some embodiments, please refer to the appendix of the specification. Figures 1-9 , a nucleic acid purification device that is easy to operate, comprising a nucleic acid purification device housing 1;

[0039] The housing 1 of the nucleic acid purification device is provided with a stirring assembly 5 that is easy to disassemble and replace for stirring and mixing, and the stirring assembly 5 that is easy to disassemble and replace is connected to the waste liquid collection assembly 4;

[0040] The easily disassembled and replaced stirring assembly 5 includes a driving device 51 and an easily disassembled stirring device 52. The nucleic acid purification device housing 1 is connected to the driving device 51. The driving device 51 is connected to the easily disassembled stirring device 52 and the waste liquid collection assembly 4.

[0041] The driving device 51 includes a T-shaped support plate 511, a second motor 512, a pulley 513, a belt 514, a mounting plate 515, a support block 516 and a plastic sleeve 517. The T-shaped support plate 511 is fixedly connected to the top of the nucleic acid purification device housing 1. The right end of the T-shaped support plate 511 is fixedly connected to the second motor 512. The output end of the second motor 512 passes through the T-shaped support plate 511 and is fixedly connected to the pulley 513 at the upper end. The left side of the lower end of the T-shaped support plate 511 is rotatably connected to the pulley 513. A pair of pulleys 513 are connected through a belt 514. The outer wall of the belt 514 is fixedly connected to the support block 516. The lower end of the support block 516 is fixedly connected to the mounting plate 515. The lower end of the mounting plate 515 is slidably connected to the plastic sleeve 517. The T-shaped support plate 511 is connected to the stirring device 52 which is easy to disassemble. The T-shaped support plate 511 is connected to the waste liquid collection component 4;

[0042] The stirring device 52 that is easy to disassemble includes a cylinder 521, a fixing plate 522, a magnetic rod 523, a clamping block 524, a spring 525, a locking groove 526, a limiting column 527 and a T-shaped slide 528. The front end of the T-shaped support plate 511 is fixedly connected to the cylinder 521, and the output end of the cylinder 521 is fixedly connected to the fixing plate 522. The lower end of the fixing plate 522 is slidably connected to the magnetic rod 523. T-shaped slides 528 are provided at both ends of the fixing plate 522. The clamping block 524 is slidably connected in the T-shaped slide 528. The locking groove 526 is provided at the upper end of the T-shaped slide 528. The outer wall of the clamping block 524 is fixedly connected to the limiting column 527. The limiting column 527 is slidably connected in the locking groove 526. The inner wall of the larger end of the T-shaped slide 528 is fixedly connected to one end of the spring 525, and the other end of the spring 525 is fixedly connected to the clamping block 524.

[0043] In the embodiment of the present utility model, when the device starts to be installed, the magnetic bead rod 523 of the easily detachable stirring device 52 of the easily detachable and replaceable stirring assembly 5 is slid into the fixing plate 522. At this time, by pressing the second clamping block 524, the limiting column 527 is driven to slide along the locking groove 526 while compressing the second spring 525 to contract. When the second clamping block 524 clamps the magnetic bead rod 523, by rotating the second clamping block 524, the limiting column 527 is driven to rotate in the locking groove 526 to lock the second clamping block 524, which is convenient for operating and replacing the magnetic bead rod. When the device is ready to stir the materials, power is provided by the second motor 512 on the T-shaped support plate 511 of the driving device 51. The upper pulley 513 is driven to rotate by the output end of the second motor 512. The upper pulley 513 drives the lower pulley 513 to rotate through the belt 514. The support block 516 and the mounting plate 515 are driven to move by the rotation of the belt 514. The plastic sleeve 517 is driven to move downward by the movement of the mounting plate 515 and inserted into the reagent kit for stirring. When the device is ready to adsorb the magnetic beads, power is provided by the cylinder 521. The fixing plate 522 is driven to move downward by the output end of the cylinder 521. The magnetic bead rod 523 is driven to move downward by the movement of the fixing plate 522. The magnetic bead rod 523 is inserted into the plastic sleeve 517 downward to adsorb the magnetic beads in the reagent kit. By quickly replacing the magnetic bead rod 523, it is avoided that the magnetic adsorption ability of the magnetic bead rod 523 is reduced after long-term use, resulting in a decrease in the purification effect.

[0044] A waste liquid collection assembly 4 for preventing waste liquid from dripping is installed on the easily detachable and replaceable stirring assembly 5;

[0045] The waste liquid collection assembly 4 includes a first motor 41, a rotating shaft 42, a baffle 43 and a sponge 44. The first motor 41 is fixedly connected to the rear end of the T-shaped support plate 511. The output end of the first motor 41 passes through the T-shaped support plate 511 and is fixedly connected to the rotating shaft 42. The outer wall of the rotating shaft 42 is fixedly connected to the baffle 43. The left end of the baffle 43 is fixedly connected to the sponge 44;

[0046] In the embodiment of the present utility model, when the device finishes stirring, power is provided by the first motor 41 of the waste liquid collection assembly 4. The rotating shaft 42 is driven to rotate by the output end of the first motor 41. The baffle 43 is driven to rotate by the rotation of the rotating shaft 42. The sponge 44 is driven to rotate to the directly below of the plastic sleeve 517 by the rotation of the baffle 43. The liquid dripping when the plastic sleeve 517 moves upward is absorbed by the sponge 44. By absorbing the nucleic acid purification extraction solution, it is avoided that the liquid drips into the device interior, causing pollution to the device and making impurities likely to appear in the subsequent purification.

[0047] A fixing assembly 3 for fixing and clamping is installed on the housing 1 of the nucleic acid purification device;

[0048] The fixed component 3 includes a rotary table 31, a limit block 32, a limit chute 33, a first clamping block 34, a slider 35, and a first spring 36. The rotary table 31 is fixedly connected to the inner bottom of the nucleic acid purification device housing 1. A plurality of pairs of limit blocks 32 are fixedly connected to the upper end of the rotary table 31 at equal intervals. A pair of limit chutes 33 are provided on each of the limit blocks 32. A pair of sliders 35 are slidably connected in the limit chutes 33. A first clamping block 34 is fixedly connected to the opposite ends of the pair of sliders 35. One end of the first spring 36 is fixedly connected to the inner wall of the limit chute 33, and the other end of the first spring 36 is fixedly connected to the first clamping block 34;

[0049] In the embodiment of the present invention, when the device starts to install the reagent kit, by inserting the reagent kit into the limit block 32, the first spring 36 pushes the first clamping block 34 to move outward along the limit chute 33 to clamp the reagent kit. The movement of the first clamping block 34 drives the slider 35 to move along the limit chute 33 to prevent the first clamping block 34 from shifting when moving. The rotary table 31 rotates to transport the next reagent kit to the purification location. By clamping and fixing the reagent kit, it is avoided that the reagent kit shifts or flips during the stirring and purification of the device, resulting in extraction failure.

[0050] A door assembly 2 for isolation and sealing is installed on the nucleic acid purification device housing 1;

[0051] The door assembly 2 includes a sealing door 21, a handle 22, and a hinge 23. A pair of hinges 23 are fixedly connected to the front end of the nucleic acid purification device housing 1. The sealing door 21 is rotatably connected to the lower ends of the pair of hinges 23. The handle 22 is fixedly connected to the lower side of the front end of the sealing door 21;

[0052] In the embodiment of the present invention, when the device starts to purify, by pulling the handle 22 of the door assembly 2 to drive the sealing door 21 to move, the sealing door 21 rotates on the nucleic acid purification device housing 1 through the hinge 23 to complete the sealing. By sealing the device, it is avoided that foreign impurities float into the device during purification, causing pollution and resulting in purification failure.

[0053] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements will not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nucleic acid purification device that is easy to operate, comprising a nucleic acid purification device housing (1), characterized in that: The housing (1) of the nucleic acid purification device is provided with a stirring assembly (5) which is easy to disassemble and replace and is convenient for stirring and mixing; The easily disassembled and replaced stirring assembly (5) comprises a driving device (51) and an easily disassembled stirring device (52); the nucleic acid purification device housing (1) is connected to the driving device (51), and the driving device (51) is connected to the easily disassembled stirring device (52); The stirring assembly (5) that is easy to disassemble and replace is equipped with a waste liquid collecting assembly (4) that prevents waste liquid from dripping; A fixing component (3) for fixing and clamping is installed on the housing (1) of the nucleic acid purification device; A door assembly (2) for isolation and sealing is installed on the housing (1) of the nucleic acid purification device.

2. The nucleic acid purification device convenient for operation according to claim 1, wherein The driving device (51) comprises a T-shaped support plate (511), a second motor (512), a pulley (513), a belt (514), a mounting plate (515), a support block (516) and a plastic sleeve (517). The top of the nucleic acid purification device housing (1) is fixedly connected with the T-shaped support plate (511), the right end of the T-shaped support plate (511) is fixedly connected with the second motor (512), the output end of the second motor (512) passes through the T-shaped support plate (511) and is fixedly connected to the pulley (513) at the upper end, the left side of the lower end of the T-shaped support plate (511) is rotatably connected with the pulley (513), a pair of pulleys (513) are connected by a belt (514), the outer wall of the belt (514) is fixedly connected with the support block (516), the lower end of the support block (516) is fixedly connected with the mounting plate (515), and the lower end of the mounting plate (515) is slidably connected with the plastic sleeve (517).

3. The nucleic acid purification device facilitating operation according to claim 2, wherein, The T-shaped support plate (511) is connected to a stirring device (52) that is easy to disassemble.

4. The nucleic acid purification device that is convenient to operate according to claim 3, wherein, The T-shaped support plate (511) is connected to the waste liquid collection assembly (4).

5. The nucleic acid purification device convenient for operation according to claim 4, wherein The stirring device (52) that is easy to disassemble comprises a cylinder (521), a fixing plate (522), a magnetic rod (523), a second clamping block (524), a second spring (525), a locking groove (526), a limiting column (527) and a T-shaped sliding groove (528). The front end of the T-shaped supporting plate (511) is fixedly connected to the cylinder (521), the output end of the cylinder (521) is fixedly connected to the fixing plate (522), the lower end of the fixing plate (522) is slidably connected to the magnetic rod (523), and the fixing plate (522) is fixedly connected to the output end of the cylinder (521). Both left and right ends are provided with T-shaped slots (528), a clamping block 2 (524) is slidably connected in the T-shaped slots (528), a locking slot (526) is provided at the upper end of the T-shaped slots (528), the outer wall of the clamping block 2 (524) is fixedly connected to a limiting column (527), the locking slot (526) is slidably connected to the limiting column (527), the inner wall of the larger end of the T-shaped slots (528) is fixedly connected to one end of the spring 2 (525), and the other end of the spring 2 (525) is fixedly connected to the clamping block 2 (524).

6. The nucleic acid purification device facilitating operation according to claim 5, wherein, The waste liquid collection component (4) includes a first motor (41), a rotating shaft (42), a baffle (43) and a sponge (44). A first motor (41) is fixedly connected to the rear end of the T-shaped support plate (511). The output end of the first motor (41) passes through the T-shaped support plate (511) and is fixedly connected to a rotating shaft (42). A baffle (43) is fixedly connected to the outer wall of the rotating shaft (42), and a sponge (44) is fixedly connected to the left end of the baffle (43).

7. The nucleic acid purification device facilitating operation according to claim 6, wherein The fixing component (3) includes a rotating table (31), a limiting block (32), a limiting sliding groove (33), a first clamping block (34), a sliding block (35) and a first spring (36). A rotating table (31) is fixedly connected to the inner bottom of the nucleic acid purification device housing (1). A plurality of pairs of limiting blocks (32) are fixedly connected to the upper end of the rotating table (31) at equal intervals. A pair of limiting sliding grooves (33) are formed in each of the limiting blocks (32). A pair of sliding blocks (35) are slidably connected in the limiting sliding grooves (33). A first clamping block (34) is fixedly connected to the opposite ends of the pair of sliding blocks (35). One end of a first spring (36) is fixedly connected to the inner wall of the limiting sliding groove (33), and the other end of the first spring (36) is fixedly connected to the first clamping block (34).

8. The nucleic acid purification device facilitating operation according to claim 7, wherein, The door component (2) includes a sealing door (21), a handle (22) and a hinge (23). A pair of hinges (23) are fixedly connected to the front end of the nucleic acid purification device housing (1). A sealing door (21) is rotatably connected to the lower ends of the pair of hinges (23). A handle (22) is fixedly connected to the lower side of the front end of the sealing door (21).

Images