Western blot detection device

Abstract

The utility model provides a western blot detection device. Relates to the technical field of western blot detection. The western blot detection device comprises a substrate and a detection base station arranged above the substrate, the bottom of the detection base station is fixedly provided with a U-shaped support frame, the U-shaped support frame is in sliding connection with the base station, the top of the detection base station is provided with two upper covers, the two upper covers are in contact with each other, and the two upper covers are in contact with each other. A plurality of reaction tanks are formed in the top of the detection base station, flat-bottom grooves are formed in the inner walls of the bottoms of the reaction tanks, bearing pieces are arranged in the flat-bottom grooves and make contact with the inner walls of the flat-bottom grooves, western blot membranes are placed on the bearing pieces, and the western blot membranes are arranged in the flat-bottom grooves. And the diameter of the supporting sheet is smaller than that of the western blot membrane. The western blot membrane clamping device has the advantage that the western blot membrane is prevented from being stabbed in the clamping process to the maximum extent.

Description

Technical Field

[0001] The utility model relates to the technical field of western blot detection, in particular to a western blot detection device. Background Technique

[0002] The western blot technique is a laboratory technique used to detect protein expression levels, quantify, and determine molecular weights. Its main principle is to color the protein samples treated by gel electrophoresis with specific antibodies, and analyze the coloring position and depth to obtain protein expression information. It usually includes steps such as sample preparation, electrophoresis, membrane transfer, detection, and image acquisition.

[0003] For example, a western blot detection device is proposed in the patent document with the authorization announcement number CN213544587U. This western blot detection device places a rubber ring between the upper cover plate and the lower cover plate, and then uses a clamping assembly to press the upper cover plate and the lower cover plate tightly, so that the antibody and the western blot membrane can be in full contact. Moreover, the rubber ring and the upper cover plate and the lower cover plate form a regular three-dimensional space, and there is no situation where the antibody and the western blot membrane are in uneven contact due to narrow corners. Moreover, when gently shaking on a shaker, the liquid and the western blot membrane can be in full contact.

[0004] However, it is found during the use of the above detection device that since the western blot membrane is inside the rubber ring and is very close to or even in contact with the inner wall of the rubber ring, when it is necessary to take out the western blot membrane from the rubber ring, it is necessary to insert forceps into it from above the rubber ring for clamping. The relatively sharp forceps are likely to pierce the blot membrane vertically from above, thus affecting subsequent detections.

[0005] Therefore, it is necessary to provide a new western blot detection device to solve the above technical problems. Content of the Utility Model

[0006] The purpose of the utility model is to provide a western blot detection device that can prevent the western blot membrane from being pierced during the clamping process to the greatest extent.

[0007] To solve the above technical problems, the protein blotting detection device provided by the utility model includes: a substrate and a detection base disposed above the substrate. A U-shaped support frame is fixedly installed at the bottom of the detection base, and the U-shaped support frame is slidably connected to the base. Two upper covers are provided on the top of the detection base, and the two upper covers are in contact with each other. A plurality of reaction grooves are formed on the top of the detection base, and flat bottom grooves are formed on the inner walls of the bottoms of the plurality of reaction grooves. Support sheets are provided in the plurality of flat bottom grooves, and the support sheets are in contact with the inner walls of the flat bottom grooves. Protein blotting membranes are placed on the plurality of support sheets. The diameter of the support sheet is smaller than the diameter of the protein blotting membrane. A lifting plate is disposed inside the U-shaped support frame. A plurality of guide posts are fixedly installed on the top of the lifting plate. The top ends of the plurality of guide posts respectively extend into the plurality of flat bottom grooves and are fixedly connected to the corresponding support sheets, and the guide posts are slidably connected to the bottom of the detection base. A toothed column is fixedly installed at the bottom of the lifting plate. A back plate is fixedly installed at the bottom of the detection base, and a rotating shaft is rotatably installed on the back plate. A gear is fixedly sleeved on the rotating shaft, and the gear meshes with the toothed column.

[0008] Preferably, a plurality of liquid inlet holes are formed on the upper cover, and the plurality of liquid inlet holes are respectively communicated with the plurality of reaction grooves.

[0009] Preferably, a first reduction motor is fixedly installed on the top of the substrate. A disc is fixedly sleeved on the output shaft of the first reduction motor. An eccentric rod is fixedly installed on the disc. A hinge arm is rotatably installed on the eccentric rod, and one side of the hinge arm is hinged to the U-shaped support frame.

[0010] Preferably, two slide rails are fixedly installed on the top of the substrate. Sliders are slidably installed on the two slide rails. The tops of the two sliders are fixedly connected to the U-shaped support frame. A second reduction motor is fixedly installed on one side of the back plate, and the output shaft of the second reduction motor is fixedly connected to one end of the rotating shaft.

[0011] Preferably, a concave seat is fixedly installed on the outer wall of one side of the U-shaped support frame. A bidirectional screw rod is rotatably installed in the concave seat. Two linkage pieces are threadedly installed on the bidirectional screw rod. The tops of the two linkage pieces are fixedly installed with connecting folding rods, and the end parts of the two connecting folding rods are respectively fixedly connected to the two upper covers.

[0012] Preferably, a third reduction motor is fixedly installed on the outer wall of one side of the concave seat. The output shaft of the third reduction motor is fixedly connected to one end of the bidirectional screw rod. Two limiting slide rods are fixedly installed in the concave seat. The two limiting slide rods penetrate through the two linkage pieces and are slidably connected to the two linkage pieces.

[0013] Compared with the related technologies, the protein blotting detection device provided by the present utility model has the following beneficial effects:

[0014] The present utility model provides a protein blotting detection device. Through the meshing between the arranged gear and the tooth column, the operation of the second reduction motor can be utilized to make the lifting plate drive the supporting piece to rise, thereby being able to eject the protein blotting membrane from the reaction tank. Moreover, the ejected protein blotting membrane protrudes from the supporting piece, so that it can be quickly and safely clamped away, providing guarantee for the subsequent detection results. And, through the rotation of the disc, the articulated arm can be utilized to drive the detection base to move reciprocally, enabling the antibody solution to fully contact and mix with the protein blotting membrane, improving the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic structural diagram of a preferred embodiment of the protein blotting detection device provided by the present utility model;

[0016] Figure 2 It is a front view structural diagram of the present utility model;

[0017] Figure 3 It is a schematic diagram of the placement position of the protein blotting membrane in the present utility model;

[0018] Figure 4 It is a schematic diagram of the opening structure of the flat bottom groove in the present utility model;

[0019] Figure 5 It is a schematic diagram of the connection structure between the lifting plate and the tooth column in the present utility model;

[0020] Figure 6 It is a schematic diagram of the connection structure between the upper cover and the connecting folding rod in the present utility model.

[0021] Reference numerals in the figures: 1, substrate; 2, U-shaped support frame; 3, detection base; 4, upper cover; 5, reaction tank; 6, liquid inlet hole; 7, flat bottom groove; 8, supporting piece; 9, lifting plate; 10, guiding column; 11, tooth column; 12, back plate; 13, rotating shaft; 14, gear; 15, first reduction motor; 16, disc; 17, eccentric rod; 18, articulated arm; 19, concave seat; 20, bidirectional screw; 21, linkage piece; 22, connecting folding rod; 23, protein blotting membrane. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0023] Please refer to Figures 1 - 6 , wherein, Figure 1 It is a schematic structural diagram of a preferred embodiment of the protein blotting detection device provided by the present utility model; Figure 2Front view structural diagram of the present utility model; Figure 3 Schematic diagram of the placement position of the protein blotting membrane in the present utility model;

[0024] Figure 4 Schematic diagram of the opening structure of the flat bottom groove in the present utility model; Figure 5 Schematic diagram of the connection structure between the lifting plate and the toothed column in the present utility model; Figure 6 Schematic diagram of the connection structure between the upper cover and the connecting folding rod in the present utility model. The protein blotting detection device includes: a substrate 1 and a detection base 3 disposed above the substrate 1. A U-shaped support frame 2 is fixedly installed at the bottom of the detection base 3, and two slide rails are fixedly installed at the top of the substrate 1. Sliders are slidably installed on both slide rails, and the tops of the two sliders are fixedly connected to the U-shaped support frame 2, enabling the U-shaped support frame 2 to move linearly on the substrate 1. A plurality of reaction grooves 5 are opened at the top of the detection base 3, flat bottom grooves 7 are opened on the bottom inner walls of the plurality of reaction grooves 5, support sheets 8 are provided in the plurality of flat bottom grooves 7, the support sheets 8 are in contact with the inner walls of the flat bottom grooves 7, protein blotting membranes 23 are placed on the plurality of support sheets 8, and the diameter of the support sheet 8 is smaller than the diameter of the protein blotting membrane 23. In this way, after the protein blotting membrane 23 is pushed out of the reaction groove 5, its outer edge protrudes from the support sheet 8, facilitating people to safely clamp it. Two upper covers 4 are provided at the top of the detection base 3, the two upper covers 4 are in contact to cover the reaction grooves 5, and a plurality of liquid inlet holes 6 are opened on the upper covers 4, and each liquid inlet hole 6 corresponds to a reaction groove 5 respectively, so that the antibody solution can be smoothly injected into it. A lifting plate 9 is provided in the U-shaped support frame 2, a plurality of guide columns 10 are fixedly installed at the top of the lifting plate 9, the tops of the plurality of guide columns 10 respectively extend into the plurality of flat bottom grooves 7 and are fixedly connected to the corresponding support sheets 8, and the guide columns 10 are slidably connected to the bottom of the detection base 3. A toothed column 11 is fixedly installed at the bottom of the lifting plate 9, a back plate 12 is fixedly installed at the bottom of the detection base 3, a rotating shaft 13 is rotatably installed on the back plate 12, a gear 14 is fixedly sleeved thereon, and the gear 14 meshes with the toothed column 11. By using their meshing, the support sheet 8 can be driven to rise and fall, and a second reduction motor is fixedly installed on one side of the back plate 12, and its output shaft is fixedly connected to one end of the rotating shaft 13.

[0025] In the above method, in order to drive the U-shaped support frame 2 to perform reciprocating motion to ensure that the antibody solution can be fully mixed with the protein blotting membrane 23, a first reduction motor 15 is fixedly installed at the top of the substrate 1, a disc 16 is fixedly sleeved on its output shaft, an eccentric rod 17 is fixedly installed on the disc 16, a hinge arm 18 is rotatably installed on the eccentric rod 17, and one side of the hinge arm 18 is hinged to the U-shaped support frame 2.

[0026] In this method, in order to be able to open the upper cover 4 and facilitate ejecting the protein blotting membrane 23 from the reaction tank 5, a concave seat 19 is fixedly installed on the outer wall of one side of the U-shaped support frame 2. A bidirectional screw 20 is rotatably installed therein. Two linkage pieces 21 are threadedly installed on the bidirectional screw 20. The tops of the two linkage pieces 21 are fixedly installed with connecting folding rods 22. The end parts of the two connecting folding rods 22 are respectively fixedly connected to the two upper covers 4. And a third reduction motor is fixedly installed on the outer wall of one side of the concave seat 19, and its output shaft is fixedly connected to one end of the bidirectional screw 20. And two limiting sliding rods are fixedly installed in the concave seat 19. The two limiting sliding rods penetrate through the two linkage pieces 21 and are slidably connected to the two linkage pieces 21, playing a limiting effect on the linkage pieces 21.

[0027] The working principle of the protein blotting method detection device provided by the present utility model is as follows:

[0028] During use, first inject specific antibody liquid into the plurality of reaction tanks 5 through the plurality of liquid inlet holes 6, so that the protein blotting membrane 23 is fused with the specific antibody solution to form an incubation process;

[0029] During the fusion process of the two, start the first reduction motor 15, and its output shaft drives the disc 16 to rotate. At this time, by using the hinged movement function of the hinged arm 18, the U-shaped support frame 2 can make a linear reciprocating movement along with the circular motion of the disc 16, so that the injected antibody solution can be sloshed back and forth to form a shaking effect, enabling the protein blotting membrane 23 to fully contact the antibody solution;

[0030] When it is necessary to take out the protein blotting membrane 23 after the fusion is completed, first start the third reduction motor in the forward direction, and its output shaft drives the bidirectional screw 20 to rotate, so that the two linkage pieces 21 move in a direction away from each other. Then, driven by the connecting folding rod 22, the two upper covers 4 move away from each other, and finally the plurality of reaction tanks 5 are opened. Then start the second reduction motor in the forward direction, and its output shaft drives the rotating shaft 13 to rotate, and then can drive the gear 14 to rotate. As the gear 14 rotates, the tooth column 11 meshing with it will drive the lifting plate 9 to rise, and then the supporting piece 8 will drive the protein blotting membrane 23 to rise until the protein blotting membrane 23 rises above the detection base 3. Then turn off the second reduction motor. At this time, the outer edge of the protein blotting membrane 23 protrudes from the supporting piece 8, and then it can be clamped from the side and safely removed.

[0031] Compared with the related technology, the protein blotting method detection device provided by the present utility model has the following beneficial effects:

[0032] The utility model provides a protein blotting detection device. Through the meshing between the gear 14 and the tooth column 11, the operation of the second reduction motor can be utilized to make the lifting plate 9 drive the supporting sheet 8 to rise, so as to eject the protein blotting membrane 23 from the reaction tank 5. Moreover, the ejected protein blotting membrane 23 protrudes from the supporting sheet 8, and thus it can be quickly and safely clamped away, ensuring the subsequent detection results. In addition, through the rotation of the disc 16, the articulated arm 18 can be utilized to drive the detection base 3 to move reciprocally, enabling the antibody solution to fully contact and mix with the protein blotting membrane 23, improving the accuracy of the detection results.

[0033] The above are only the embodiments of the present utility model, and do not limit the patent scope of the present utility model accordingly. Any equivalent structural or equivalent process transformation made by using the content of the specification and drawings of the present utility model, or directly or indirectly applied in other related technical fields, shall be similarly included in the patent protection scope of the present utility model.

Claims

1. A Western blot detection device, comprising a substrate and a detection base station arranged above the substrate, characterized in that, A U-shaped support frame is fixedly installed at the bottom of the detection base platform. The U-shaped support frame is slidably connected to the base platform. Two upper covers are provided on the top of the detection base platform, and the two upper covers are in contact with each other. A plurality of reaction grooves are formed in the top of the detection base platform. Flat bottom grooves are formed in the inner walls of the bottoms of the plurality of reaction grooves. Support sheets are arranged in the plurality of flat bottom grooves, and the support sheets are in contact with the inner walls of the flat bottom grooves. Protein blotting membranes are placed on the plurality of support sheets. The diameter of the support sheet is smaller than the diameter of the protein blotting membrane. A lifting plate is arranged in the U-shaped support frame. A plurality of guide columns are fixedly installed on the top of the lifting plate. The top ends of the plurality of guide columns respectively extend into the plurality of flat bottom grooves and are fixedly connected to the corresponding support sheets, and the guide columns are slidably connected to the bottom of the detection base platform. A toothed column is fixedly installed at the bottom of the lifting plate. A back plate is fixedly installed at the bottom of the detection base platform. A rotating shaft is rotatably installed on the back plate. A gear is fixedly sleeved on the rotating shaft, and the gear is meshed with the toothed column.

2. The protein blotting detection device according to claim 1, wherein A plurality of liquid inlet holes are formed in the upper cover, and the plurality of liquid inlet holes are respectively communicated with the plurality of reaction grooves.

3. The western blot detection device according to claim 1, wherein A first reduction motor is fixedly installed on the top of the substrate. A disc is fixedly sleeved on the output shaft of the first reduction motor. An eccentric rod is fixedly installed on the disc. A hinge arm is rotatably installed on the eccentric rod. One side of the hinge arm is hinged to the U-shaped support frame.

4. The Western blot detection device according to claim 1, wherein, Two slide rails are fixedly installed on the top of the substrate. Sliders are slidably installed on the two slide rails. The tops of the two sliders are fixedly connected to the U-shaped support frame. A second reduction motor is fixedly installed on one side of the back plate. The output shaft of the second reduction motor is fixedly connected to one end of the rotating shaft.

5. The protein blotting detection device according to claim 1, wherein A concave seat is fixedly installed on the outer wall of one side of the U-shaped support frame. A bidirectional screw rod is rotatably installed in the concave seat. Two linkage pieces are threadedly installed on the bidirectional screw rod. The tops of the two linkage pieces are fixedly installed with connecting folding rods. The end parts of the two connecting folding rods are respectively fixedly connected to the two upper covers.

6. The Western blot detection device according to claim 5, characterized in that, A third reduction motor is fixedly installed on the outer wall of one side of the concave seat. The output shaft of the third reduction motor is fixedly connected to one end of the bidirectional screw rod. Two limiting slide rods are fixedly installed in the concave seat. The two limiting slide rods penetrate through the two linkage pieces and are slidably connected to the two linkage pieces.

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