Novel urokinase titer measuring equipment
By designing the urokinase titer measurement equipment for feeding plates, collection components and fixing components, the problem of sample spilling of the microplate reader is solved, the automatic sample collection and equipment stability and sealing are achieved, and the practicality and accuracy of the detection are improved.
Patent Information
- Application Number
- CN202422338696.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-25
- Publication Date
- 2025-08-01
- Estimated Expiration
- 2034-09-25
AI Technical Summary
Existing microplate reader equipment can easily cause samples to spill during sampling, affecting detection accuracy and equipment cleaning.
A new type of urokinase titer measurement equipment is designed, using feeding plate, collection assembly and fixing assembly. Through the cooperation of the rotating rod and helical gear, the samples are automatically collected, avoiding manual pickup, and a protective plate and spring are set up for sealing.
It improves the practicality of the sample measurement process, avoids the spilling of samples, ensures detection accuracy and equipment cleaning, and enhances the stability and sealing of the equipment.
Smart Images
Figure CN223180220U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of urokinase detection, in particular to a novel device for determining the titer of urokinase. Background Art
[0002] Urokinase is an alkaline proteolytic hydrolate isolated and purified from fresh human urine of healthy people. It can directly act on the endogenous fibrinolytic system, catalyze the cleavage of plasminogen into plasmin. The determination of urokinase titer is one of the important steps in its preparation process, and the activity of urokinase is directly related to its efficacy. An enzyme-labeled instrument is usually used to detect the activity of urokinase during the determination.
[0003] In the prior art, for some enzyme-labeled instrument devices used for the determination of urokinase titer, during the determination, the sample needs to be dropped onto the inner wall of the groove of the groove plate, and then the groove plate is placed on the movable support plate of the device. The sample is sent into the enzyme-labeled instrument for detection through the movable support plate. To ensure the stability of the groove plate and the accuracy of positioning, a groove for positioning the groove plate is generally opened on the movable support plate. However, this will cause the groove plate to tilt when taking out the groove plate after the detection is completed, resulting in the sample spilling onto the support plate, which may affect the accuracy of the next detection, and the practicability is poor. Therefore, a novel device for determining the titer of urokinase is proposed to solve the above problems. Summary of the Utility Model
[0004] In order to make up for the above deficiencies, the utility model provides a novel device for determining the titer of urokinase, aiming to improve the problem that the sample is likely to accidentally spill onto the device during the process of using an enzyme-labeled instrument to determine urokinase in the prior art.
[0005] To achieve the above purpose, the utility model adopts the following technical scheme: A novel device for determining the titer of urokinase, including a detector. A feeding plate is arranged on the inner wall of the front end of the detector. A second through groove is opened on the lower surface of the feeding plate. A limiting groove is opened at the bottom end of the front surface of the detector. A placing component is arranged on the inner wall of the second through groove. A collecting component is arranged on the lower side of the front surface of the detector adjacent to the feeding plate. A fixing component is arranged on the right surface of the collecting component. The placing component includes a connecting block. The connecting block slides on the inner wall of the second through groove. A baffle is fixedly connected to the left surface of the connecting block. A rotating rod is fixedly connected through the front surface of the connecting block. The rotating rod passes through and is rotatably connected to the right end of the front surface of the feeding plate. The collecting component includes a collecting box. The collecting box is in contact with the front surface of the detector.
[0006] As a further description of the above technical solution:
[0007] The collection component further includes a protective plate, which is slidably connected to the inner wall of the top of the collection box. Through grooves I are opened on the front and rear sides of the inner wall of the top of the collection box, and the left end of the front side of the protective plate is slidably connected to the inner wall of the through groove I.
[0008] As a further description of the above technical solution:
[0009] The fixing component includes a fixing column, which is fixedly connected to the right surface of the collection box. A rotating rod is rotatably connected through the front surface of the fixing column, and a connecting handle is fixedly connected to the front surface of the rotating rod.
[0010] As a further description of the above technical solution:
[0011] An external wall at the front end of the rotating rod is fixedly connected with a helical gear. A torsion spring I is sleeved on the outer wall of the rotating rod near the rear side of the helical gear. One end of the torsion spring I is fixedly connected to the inner wall of the through groove II, and the other end of the torsion spring I is fixedly connected to the front surface of the connecting block.
[0012] As a further description of the above technical solution:
[0013] The right surface of the front end of the protective plate is fixedly connected with a spring, and the right end of the spring is fixedly connected to the inner wall of the through groove I.
[0014] As a further description of the above technical solution:
[0015] A limiting plate is fixedly connected to the rear surface of the rotating rod, and the limiting plate contacts the inner wall of the limiting groove.
[0016] As a further description of the above technical solution:
[0017] A torsion spring II is fixedly connected to the outer wall of the front end of the rotating rod. One end of the torsion spring II is fixedly connected to the rear surface of the connecting handle, and the other end of the torsion spring II is fixedly connected to the front surface of the fixing column.
[0018] As a further description of the above technical solution:
[0019] Multiple groups of the fixing components are provided, and the multiple groups of fixing components are symmetrically arranged with the center line of the collection box as the axis of symmetry.
[0020] The utility model has the following beneficial effects:
[0021] 1. In the utility model, after the sample determination is completed, only the rotating rod needs to be rotated, and the baffle will rotate accordingly, so that the sample together with the sample placement plate will fall into the interior of the collection box, without the need for the measuring personnel to take it out manually, avoiding sample spillage and pollution to the equipment, and improving the practicability.
[0022] 2. In the present utility model, through the arranged protective plate and spring, the collection box can be sealed to a certain extent, avoiding the odor of the samples inside the collection box from escaping, improving the practicability. At the same time, through the arranged fixing component, the stability of the collection box can be ensured, improving the practicability. Description of the Drawings
[0023] Figure 1 It is a schematic diagram of the three-dimensional structure of the overall device in the present utility model;
[0024] Figure 2 It is a schematic diagram of the split cross-section of the three-dimensional structure of the measuring instrument and the collection box in the present utility model;
[0025] Figure 3 It is a schematic diagram of the split cross-section of the three-dimensional structure of the feeding plate and the baffle in the present utility model;
[0026] Figure 4 It is a schematic diagram of the bottom view of the three-dimensional structure of the feeding plate in the present utility model;
[0027] Figure 5 In the present utility model Figure 2 The enlarged schematic diagram of the three-dimensional structure of area A;
[0028] Figure 6 In the present utility model Figure 3 The enlarged schematic diagram of the three-dimensional structure of area B.
[0029] Legend Explanation:
[0030] 1. Measuring instrument; 2. Feeding plate; 31. Rotating rod; 32. Baffle; 33. Connecting block; 34. Helical gear; 35. First torsion spring; 41. Collection box; 42. Protective plate; 43. Spring; 44. First through groove; 51. Fixed column; 52. Connecting handle; 53. Second torsion spring; 54. Limiting plate; 55. Rotating rod; 6. Limiting groove; 7. Second through groove. Detailed Embodiment
[0031] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.
[0032] Refer to Figure 1 - Figure 4, an embodiment provided by the present utility model: a novel determination device for urokinase titer, including a detector 1. The detector 1 is a prior art, through which the activity of urokinase can be determined, and those skilled in the art can implement it. Since it is a prior art, it will not be described in detail in this case. The inner wall of the front end of the detector 1 is provided with a feeding plate 2. The feeding plate 2 is a prior art, and the groove plate for placing samples can be placed therein and sent into the interior of the detector 1 through the feeding plate 2. At the same time, the feeding plate 2 is of a hollow design to ensure that the groove plate can fall smoothly. Four groups of through grooves two 7 are opened on the lower surface of the feeding plate 2, and the four groups are respectively arranged at the four sides of the front, rear, left and right of the lower surface of the hollow part of the feeding plate 2. A limiting groove 6 is opened at the bottom end of the front surface of the detector 1. A placing component is arranged on the inner wall of the through groove two 7. One placing component is arranged on the inner walls of multiple groups of through grooves two 7, and multiple groups of placing components are cooperatively connected through a helical gear 34 to ensure that when the rotating rod 31 rotates, the baffles 32 in multiple groups of placing components can rotate together. A collecting component is arranged on the lower side of the front surface of the detector 1 adjacent to the feeding plate 2, and a fixing component is arranged on the right surface of the collecting component.
[0033] Refer to Figure 2 - Figure 3 , Figure 6 , the placing component includes a connecting block 33 that ensures the stable rotation of the baffle 32. The connecting block 33 slides on the inner wall of the through groove two 7. The left surface of the connecting block 33 is fixedly connected with the baffle 32. When the baffle 32 is in a horizontal state, the groove plate for discharging materials can be placed on its upper surface. The front surface of the connecting block 33 is fixedly connected with a rotating rod 31 through. By rotating the rotating rod 31, the baffle 32 can be controlled to rotate, so that the measured sample falls into the interior of the collecting box 41. The rotating rod 31 penetrates and is rotatably connected to the right end of the front surface of the feeding plate 2. The collecting component includes a collecting box 41 that can collect the measured sample. The collecting box 41 contacts the front surface of the detector 1.
[0034] Refer to Figure 2 , Figure 5 , the collecting component further includes a protection plate 42. When putting the measured sample into the interior of the collecting box 41, it is necessary to pull the protection plate 42 to open it, and it will automatically close after the sample completely enters. The protection plate 42 is slidably connected to the inner wall of the top end of the collecting box 41. Through grooves one 44 are opened on the front and rear sides of the inner wall of the top end of the collecting box 41. The left end of the front side of the protection plate 42 is slidably connected to the inner wall of the through groove one 44. A spring 43 is fixedly connected to the right surface of the front end of the protection plate 42. The spring 43 always pulls the protection plate 42 to move to the right through its own elasticity, ensuring that when the protection plate 42 is opened and then closed, it can be automatically closed through the elasticity of the spring 43, improving the practicability. The right end of the spring 43 is fixedly connected to the inner wall of the through groove one 44.
[0035] Refer toFigure 1 - Figure 2 The fixing component includes a fixing column 51 that ensures the stable rotation of the rotating rod 55. The fixing column 51 is fixedly connected to the right surface of the collection box 41. The front surface of the fixing column 51 is rotatably connected through the rotating rod 55. A connecting handle 52 is fixedly connected to the front surface of the rotating rod 55. The rotating rod 55 can be smoothly rotated by pushing the connecting handle 52.
[0036] Refer to Figure 3 , Figure 6 On the outer wall of the front end of the rotating rod 31, a helical gear 34 is fixedly connected. The helical gear 34 is a prior art. When rotating, it can drive another set of helical gears 34 to rotate. A first torsion spring 35 is sleeved on the outer wall of the rotating rod 31 near the rear side of the helical gear 34. The first torsion spring 35 has a certain elasticity. It ensures that the baffle 32 always remains horizontal without rotating the rotating rod 31 through its own elasticity. One end of the first torsion spring 35 is fixedly connected to the inner wall of the second through groove 7, and the other end of the first torsion spring 35 is fixedly connected to the front surface of the connecting block 33.
[0037] Refer to Figure 1 - Figure 2 On the rear surface of the rotating rod 55, a limiting plate 54 is fixedly connected. The limiting plate 54 contacts the inner wall of the limiting groove 6. The left end of the limiting groove 6 is open, ensuring that the limiting plate 54 can be smoothly inserted into it. The front side of the right end is closed. When the limiting plate 54 is in a horizontal state in its inner wall, it can be stuck in it. A second torsion spring 53 is fixedly connected to the outer wall of the front end of the rotating rod 55. The second torsion spring 53 ensures that the limiting plate 54 always remains horizontal without rotating the rotating rod 55 through its own elasticity. One end of the second torsion spring 53 is fixedly connected to the rear surface of the connecting handle 52, and the other end of the second torsion spring 53 is fixedly connected to the front surface of the fixing column 51. Multiple groups of fixing components are provided. Setting multiple groups of fixing components ensures the stability of the collection box 41, and multiple groups of fixing components are symmetrically arranged with the center line of the collection box 41 as the axis of symmetry.
[0038] Working principle: Before measuring the urokinase titer, first place the groove plate for placing the sample on the feeding plate 2. At this time, due to the action of the first torsion spring 35, the baffle 32 remains horizontal and can stably support the groove plate. Then, start the measuring instrument 1, and the feeding plate 2 starts to work, sending the groove plate into the measuring instrument 1 for measuring the urokinase titer. The hollow design of the feeding plate 2 ensures the stability of the groove plate.
[0039] After the measurement is completed, the groove plate with the sample is sent out through the feeding plate 2. Subsequently, the operator rotates the rotating rod 31 to drive the helical gear 34 to rotate. The rotation of the rotating rod 31 causes the connecting block 33 to drive the baffle 32 to rotate. Through the cooperation of the helical gears 34 in multiple placement components, it can be ensured that multiple baffles 32 rotate together to the vertical state. The tested sample together with the groove plate is placed inside the inner wall of the collection box 41. When the rotating rod 31 rotates, it will mobilize the first torsion spring 35 to twist and deform. After the sample drops, release the rotating rod 31, and the first torsion spring 35 will drive the rotating rod 31 to rotate in the reverse direction through its own elasticity. Multiple baffles 32 and the connecting block 33 will also rotate in the reverse direction to reset.
[0040] To prevent the odor of the sample stored inside the collection box 41 from escaping, a protective plate 42 is provided. When it is necessary to place the measured sample, pull the protective plate 42, and the protective plate 42 slides on the inner wall at the top of the collection box 41. At this time, the spring 43 is stretched. After the sample completely enters the collection box 41, release the protective plate 42, and the elastic contraction force of the spring 43 will drive the protective plate 42 to automatically close, providing a certain degree of sealing for the collection box 41.
[0041] When it is necessary to process the sample inside the collection box 41, hold the connecting handle 52 and rotate the rotating rod 55, and the limiting plate 54 will also rotate accordingly, from the horizontal state to the vertical state. At the same time, the connecting handle 52 will drive the second torsion spring 53 to twist and deform, so that the collection box 41 can be disassembled. During installation, the operation is the same as above. When the limiting plate 54 is completely inserted into the inner wall of the limiting groove 6, release the connecting handle 52. The second torsion spring 53 will drive the rotating rod 55 and the limiting plate 54 to rotate in the reverse direction through its own elasticity, so that the limiting plate 54 is stuck on the inner wall of the limiting groove 6.
[0042] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A novel device for determining the potency of urokinase, comprising a measuring instrument (1), characterized in that: The inner wall at the front end of the measuring instrument (1) is provided with a feeding plate (2). A second through groove (7) is formed on the lower surface of the feeding plate (2). A limiting groove (6) is formed at the bottom end of the front surface of the measuring instrument (1). A placing component is arranged on the inner wall of the second through groove (7). A collecting component is arranged on the lower side of the front surface of the measuring instrument (1) near the feeding plate (2). A fixing component is arranged on the right surface of the collecting component. The placing component includes a connecting block (33). The connecting block (33) slides on the inner wall of the second through groove (7). A baffle (32) is fixedly connected to the left surface of the connecting block (33). A rotating rod (31) is fixedly connected through the front surface of the connecting block (33). The rotating rod (31) is rotatably connected through the right end of the front surface of the feeding plate (2). The collecting component includes a collecting box (41). The collecting box (41) is in contact with the front surface of the measuring instrument (1).
2. The determination device for the titer of a novel urokinase according to claim 1, characterized in that: The collecting component further includes a protection plate (42). The protection plate (42) is slidably connected to the inner wall of the top end of the collecting box (41). First through grooves (44) are formed on the front and rear sides of the inner wall of the top end of the collecting box (41). The left end of the front side of the protection plate (42) is slidably connected to the inner wall of the first through groove (44).
3. A novel determination device for urokinase titer according to claim 1, characterized in that: The fixing component includes a fixing column (51). The fixing column (51) is fixedly connected to the right surface of the collecting box (41). A rotating rod (55) is rotatably connected through the front surface of the fixing column (51). A connecting handle (52) is fixedly connected to the front surface of the rotating rod (55).
4. A novel determination device for urokinase titer according to claim 1, characterized in that: An external wall at the front end of the rotating rod (31) is fixedly connected with a helical gear (34). A first torsion spring (35) is sleeved on the outer wall of the rotating rod (31) near the rear side of the helical gear (34). One end of the first torsion spring (35) is fixedly connected to the inner wall of the second through groove (7). The other end of the first torsion spring (35) is fixedly connected to the front surface of the connecting block (33).
5. A novel determination device for urokinase titer according to claim 2, characterized in that: A spring (43) is fixedly connected to the right surface of the front end of the protection plate (42). The right end of the spring (43) is fixedly connected to the inner wall of the first through groove (44).
6. A novel determination device for urokinase titer according to claim 3, characterized in that: A limiting plate (54) is fixedly connected to the rear surface of the rotating rod (55). The limiting plate (54) is in contact with the inner wall of the limiting groove (6).
7. A novel determination device for urokinase titer according to claim 3, characterized in that: A second torsion spring (53) is fixedly connected to the outer wall of the front end of the rotating rod (55). One end of the second torsion spring (53) is fixedly connected to the rear surface of the connecting handle (52). The other end of the second torsion spring (53) is fixedly connected to the front surface of the fixing column (51).
8. A novel determination device for urokinase titer according to claim 1, characterized in that: Multiple groups of the fixing components are provided, and the multiple groups of fixing components are symmetrically arranged with the center line of the collecting box (41) as the axis of symmetry.