Small anaerobic fermentation integrated device capable of continuously sampling

Abstract

The small anaerobic fermentation integrated device comprises a feed liquid bottle, an operation box and a heat preservation cover, a PET filter membrane bottle cap is arranged at the upper end of the feed liquid bottle, and an openable top cap is arranged at the upper end of the PET filter membrane bottle cap. An inoculation opening with a butyl rubber plug at one end and a material supplementing opening connected with a filter and a pipe clamp are respectively formed in the surfaces of the two sides of the upper end of the feed liquid bottle, and a discharge opening is formed in one side of the lower end of the feed liquid bottle. According to the small anaerobic fermentation integrated device capable of continuously sampling, disclosed by the utility model, a relatively stable anaerobic environment can be created, so that the environment requirements of small-volume anaerobic liquid culture and anaerobic continuous sampling are met; therefore, on the premise that the anaerobic environment is not damaged in one device, liquid culture is completed, continuous sampling can be conducted at any time, the limitation that in a traditional device, an oxygen removal bag needs to be used and replaced for multiple times is avoided, the test cost is greatly reduced, and the device is practical.

Description

Technical Field

[0001] The utility model relates to the technical field of medicine and chemical industry, in particular to a small-scale anaerobic fermentation integrated device capable of continuous sampling. Background Art

[0002] In biomedical research and development, medical experiments, and other work, research anaerobic culture and fermentation cannot use large or medium-sized anaerobic fermentation tanks due to the small volume of materials. Usually, sealed anaerobic tanks or anaerobic bags with disposable deoxygenation bags are used to establish an anaerobic environment. If the research requires sampling at different time points, the existing anaerobic environment will be destroyed each time the sample is taken. After sampling, a new deoxygenation bag must be placed and the container must be sealed again to re-establish the anaerobic environment. When there are many sampling points and the time interval is short, the above operation will cause a large amount of disposable deoxygenation bags to be consumed, making the research cost high. What is more disadvantageous is that frequent sampling will cause the anaerobic environment to be incompletely established in a short period of time, causing large errors in the research results. It can be seen that the existing experimental equipment has great defects. Therefore, there is an urgent need for a small-scale anaerobic fermentation integrated device that can continuously sample without destroying the anaerobic environment. To this end, we propose a small-scale anaerobic fermentation integrated device that can continuously sample. Utility Model Content

[0003] The utility model provides a small-sized anaerobic fermentation integrated device capable of continuous sampling, which solves the technical problems raised by the background technology in the prior art.

[0004] The utility model solves the above technical problems as follows: it comprises a liquid bottle, an operation box and a heat preservation cover, the upper end of the liquid bottle is provided with a PET filter membrane bottle cap, the upper end of the PET filter membrane bottle cap is provided with an openable top cover, the two sides of the upper end of the liquid bottle are respectively provided with an inoculation port with a butyl rubber plug at one end and a feeding port connected to a filter and a tube clamp, a discharge port is provided on one side of the lower end of the liquid bottle, a rotary valve is provided at one end of the discharge port that penetrates the heat preservation cover and is fixedly connected to the penetration part, a hose is provided at one end of the rotary valve that is sleeved with the discharge port, and the liquid A ventilation pipe with a filter and connected to the operation box is provided on the other side of the lower end of the bottle, a stirrer is provided inside the liquid bottle, an electromagnetic induction sheet is provided at the upper end of the operation box, a magnetic device is provided inside the electromagnetic induction sheet, the liquid bottle is fixedly connected to the electromagnetic induction sheet through a fixed buckle, a detachable small nitrogen compression tank, a heating device and a sensor device are provided inside the operation box, the small nitrogen compression tank is connected to the ventilation pipe through a gas interface, a control panel is provided on the front side of the operation box, and a storage bag is provided on the inside of the insulation cover.

[0005] On the basis of the above technical solution, the present invention can also be improved as follows.

[0006] Furthermore, the heat preservation cover and the operation box are sealed and connected by means of a silicone groove and a sealing strip.

[0007] Furthermore, the heat-insulating cover is a transparent cover body, and a top cover 2 is provided on the upper end of the heat-insulating cover, and the top cover 2 has a sealing ring and a buckle.

[0008] Furthermore, the control panel surface is provided with a display screen, temperature control buttons, a gas control knob, a magnetic control knob and a main switch.

[0009] The beneficial effects of the present invention are as follows: the present invention provides a small-scale anaerobic fermentation integrated device capable of continuous sampling, which has the following advantages:

[0010] 1. With simple structure, ingenious design and reasonable layout, it can create a relatively stable anaerobic environment, meeting the environmental requirements of small-volume anaerobic liquid culture and anaerobic continuous sampling, thus achieving the completion of liquid culture and continuous sampling at any time in one device without destroying the anaerobic environment, breaking away from the limitation of traditional devices that must use and replace deoxygenation bags many times, greatly reducing the test cost;

[0011] 2. It integrates stirring, temperature control, ventilation, feeding and other functions into one, and can achieve inoculation, culture and sampling in an integrated manner. It can achieve liquid culture and continuous sampling in two modes, static and oscillating, under anaerobic conditions, without the need for additional incubators. In case of emergencies, it can meet the needs of continued use without being affected by changes in the external environment to a certain extent;

[0012] 3. The production process is simple, no special materials are required, the manufacturing cost is low, the volume is small, it is easy to disassemble and move, and there are no excessive requirements and restrictions on the site of use.

[0013] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and to implement it according to the contents of the description, the following preferred embodiments of the present invention are described in detail with reference to the accompanying drawings. The specific implementation methods of the present invention are given in detail in the following embodiments and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0015] Figure 1 A schematic diagram of the overall structure of a small-scale anaerobic fermentation integrated device capable of continuous sampling provided by one embodiment of the present invention;

[0016] Figure 2 for Figure 1 A schematic diagram of the internal structure of the operating box in a small-scale anaerobic fermentation integrated device capable of continuous sampling is provided.

[0017] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0018] 1. Liquid bottle; 2. Operation box; 3. Insulation cover; 4. PET filter membrane bottle cap; 5. Inoculation port; 6. Feed port; 7. Discharge port; 8. Rotary valve; 9. Vent tube; 10. Gas interface; 11. Fixing buckle; 12. Electromagnetic induction plate; 13. Stirring bar; 14. Display screen; 15. Temperature control button; 16. Gas control knob; 17. Magnetic control knob; 18. Main switch; 19. Storage bag; 20. Small nitrogen compression tank; 21. Heating device; 22. Sensor device; 23. Magnetic device. DETAILED DESCRIPTION

[0019] The following is combined with Figure 1-2 The principles and features of the present invention are described, and the examples provided are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The following paragraphs describe the present invention in more detail by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become more apparent from the following description and claims. It should be noted that the drawings are greatly simplified and not to exact scale, and are intended solely to facilitate and clearly illustrate the embodiments of the present invention.

[0020] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may also be a central component. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may also be a central component. When a component is considered to be "set on" another component, it may be directly set on the other component or there may also be a central component. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of this invention are intended only to describe specific embodiments and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0022] like Figure 1-2As shown, the utility model provides a small-scale anaerobic fermentation integrated device capable of continuous sampling, comprising a liquid bottle 1, an operation box 2 and a heat preservation cover 3, a PET filter membrane bottle cap 4 is provided on the upper end of the liquid bottle 1, an openable top cover is provided on the upper end of the PET filter membrane bottle cap 4, an inoculation port 5 with a butyl rubber plug at one end and a feeding port 6 connected to a filter and a tube clamp are respectively provided on the surfaces of the upper end of the liquid bottle 1, a discharge port 7 is provided on one side of the lower end of the liquid bottle 1, a rotary valve 8 is provided at one end of the discharge port 7 that penetrates the heat preservation cover 3 and is fixedly connected to the penetration portion, a hose is provided at one end of the rotary valve 8 that is sleeved with the discharge port 7, and the lower end of the liquid bottle 1 is provided with a butyl rubber plug. A ventilation pipe 9 with a filter and connected to the operation box 2 is provided on the other side of the end, a stirrer 13 is provided inside the liquid bottle 1, an electromagnetic induction sheet 12 is provided on the upper end of the operation box 2, a magnetic device 23 is provided inside the electromagnetic induction sheet 12, the liquid bottle 1 is fixedly connected to the electromagnetic induction sheet 12 through a fixing buckle 11, a detachable small nitrogen compression tank 20, a heating device 21 and a sensor device 22 are provided inside the operation box 2, the small nitrogen compression tank 20 is connected to the ventilation pipe 9 through a gas interface 10, a control panel is provided on the front side of the operation box 2, and a storage bag 19 is provided on the inside of the thermal insulation cover 3.

[0023] Preferably, the heat-insulating cover 3 and the operating box 2 are sealed and connected by means of a silicone groove and a sealing strip.

[0024] Preferably, the heat-insulating cover 3 is a transparent cover body, and a top cover 2 is provided on the upper end of the heat-insulating cover 3, and the top cover 2 has a sealing ring and a buckle.

[0025] Preferably, the control panel surface is provided with a display screen 14 , a temperature control button 15 , a gas control knob 16 , a magnetic control knob 17 and a main switch 18 .

[0026] The specific working principle and usage of this utility model are as follows:

[0027] The utility model provides a small-scale anaerobic fermentation integrated device capable of continuous sampling. When in use, 1 / 3 to 1 / 2 of the bottle volume of feed liquid is added to the inside of the feed liquid bottle 1. If stirring and culturing is required, a stirrer 13 is placed in the feed liquid bottle 1, the PET filter membrane bottle cap 4 is tightened, the top cover on the PET filter membrane bottle cap 4 is opened, the filter membrane ventilation mode is used, the butyl rubber plug of the inoculation port 5 is replaced with a new one, the pipe clamps of the feeding port 6 and the vent pipe 9 are clamped, if feeding is required, the feeding port 6 can be connected to the feeding bottle, the discharge port 7 is tightened by the cap, and then the entire feed liquid bottle 1 is sterilized by high-pressure steam. After sterilization, the top cover of the PET filter membrane bottle cap 4 is closed while hot, the feed liquid bottle 1 is tilted toward the side of the vent pipe 9, the cap of the discharge port 7 is quickly removed under flame protection, and the discharge port 7 is connected to the rotary valve 8 through a hose. After the connection is completed, the feed liquid bottle 1 is fixed back to the fixing buckle 11, and the vent pipe 9 is connected to the gas interface 10. Turn on the main switch 18 of the operating box 2, adjust the gas control knob 16, and loosen the pipe clamp on the vent tube 9 after about 3 seconds. Let nitrogen gas flow into the liquid bottle 1. After uniform large bubbles appear in the liquid in the liquid bottle 1, gently rotate the rotary valve 8. After the liquid flows out, quickly close the rotary valve 8. Open the top cover of the PET filter membrane bottle cap 4. Under flame protection, inject the inoculum from the inoculation port 5 into the liquid bottle 1. Close the top cover of the PET filter membrane bottle cap 4 and adjust the gas control knob 16 to stop ventilation. Place the anaerobic bag and indicator into the storage bag 19 on the inner wall of the insulation cover 3. Quickly and tightly buckle the insulation cover 3 to the operating box 2 along the silicone groove and sealing strip. The device is ready.

[0028] After the liquid is cooled, the temperature control button 15 is adjusted to set the desired temperature inside the device. The sensor device 22 controls the heating device 21 to start working, and the electromagnetic induction plate 12 is used to increase the temperature inside the device. The real-time temperature inside the device and the set temperature can be displayed on the display screen 14. If the liquid culture needs to be stirred, the magnetic control knob 17 can be adjusted to magnetize. The stirrer 13 can stir the liquid under the action of the electromagnetic induction plate 12. The electromagnetic induction plate 12 is connected to the heating device 21 and the sensor device 22 for temperature display and adjustment. A magnetic device 23 is built in the middle to control the operation of the stirrer 13 through magnetization.

[0029] When sampling is required during the culture process, the gas control knob 16 can be adjusted to ventilate the liquid bottle 1 to generate positive pressure, and then the rotary valve 8 can be opened. After obtaining the liquid, the rotary valve 8 can be closed and the gas control knob 16 can be adjusted to stop ventilation. During the preparation of the device and the initial cultivation, under the action of the anaerobic bag and the introduction of nitrogen, a good anaerobic environment will be created inside the device and the liquid bottle 1. During the subsequent multiple sampling processes, only a short ventilation is required during sampling to ensure a positive pressure inside the liquid bottle 1. No ventilation is required during the rest of the time, the gas consumption is small, and there is no need to replace the anaerobic bag. When the culture time is long or the anaerobic environment inside the liquid bottle 1 is found to be insufficient, the top cover 2 on the insulation cover 3 can be opened to replace the anaerobic bag in the storage bag 19, and the top cover 1 of the PET filter membrane bottle cap 4 can be opened. After a short ventilation, the gas control knob 16 can be adjusted to restore a good anaerobic environment.

[0030] After the cultivation is completed, it is necessary to first open the gas control knob 16 and the rotary valve 8 to discharge the liquid, then close the gas control knob 16, the magnetic control knob 17 and the main switch 18, open the top cover 2 on the thermal insulation cover 3, separate the hose at one end of the rotary valve 8 from the discharge port 7, put the cap of the discharge port 7 back on, remove the thermal insulation cover 3, take the liquid bottle 1 out of the fixing buckle 11, disinfect and clean it for next use.

[0031] After using it several times, when the nitrogen in the small nitrogen compression tank 20 is used up, the rear cover of the operation box 2 can be opened for replacement. After replacement, the rear cover can be closed and the machine can be turned on for use.

[0032] The above description is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Any ordinary technician in this industry can smoothly implement the present invention as shown in the drawings and described above. However, any equivalent changes, modifications and evolutions made by technicians familiar with this profession without departing from the scope of the technical solution of the present invention using the technical content disclosed above are all equivalent embodiments of the present invention. At the same time, any equivalent changes, modifications and evolutions made to the above embodiments based on the essential technology of the present invention are still within the scope of protection of the technical solution of the present invention.

Claims

1. A small-scale anaerobic fermentation integrated device capable of continuous sampling, comprising a liquid bottle (1), an operation box (2) and a heat-insulating cover (3), characterized in that: The upper end of the liquid bottle (1) is provided with a PET filter membrane bottle cap (4), and the upper end of the PET filter membrane bottle cap (4) is provided with an openable top cover. The surfaces of the upper end of the liquid bottle (1) are respectively provided with an inoculation port (5) with a butyl rubber plug at one end and a feeding port (6) connected to a filter and a tube clamp. A discharge port (7) is provided on one side of the lower end of the liquid bottle (1). A rotary valve (8) is provided at one end of the discharge port (7) that penetrates the heat-insulating cover (3) and is fixedly connected to the penetration portion. A rubber hose is provided at one end of the rotary valve (8) that is sleeved with the discharge port (7). A ventilation pipe (9) with a filter and connected to the operation box (2) is provided on the other side of the lower end of the liquid bottle (1). A stirring rod (13) is provided inside the liquid bottle (1), an electromagnetic induction sheet (12) is provided at the upper end of the operation box (2), a magnetic device (23) is provided inside the electromagnetic induction sheet (12), the liquid bottle (1) is fixedly connected to the electromagnetic induction sheet (12) through a fixing buckle (11), a detachable small nitrogen compression tank (20), a heating device (21) and a sensor device (22) are provided inside the operation box (2), the small nitrogen compression tank (20) is connected to the ventilation pipe (9) through a gas interface (10), a control panel is provided on the front side of the operation box (2), and a storage bag (19) is provided inside the heat-insulating cover (3).

2. A small-scale anaerobic fermentation integrated device capable of continuous sampling according to claim 1, characterized in that: The heat-insulating cover (3) and the operating box (2) are sealed and connected by means of a silica gel groove and a sealing strip.

3. A small-scale anaerobic fermentation integrated device capable of continuous sampling according to claim 1, characterized in that: The heat-insulating cover (3) is a transparent cover body, and a second top cover is provided at the upper end of the heat-insulating cover (3), and the second top cover is provided with a sealing ring and a buckle.

4. A small-scale anaerobic fermentation integrated device capable of continuous sampling according to claim 1, characterized in that: The control panel surface is provided with a display screen (14), a temperature control button (15), a gas control knob (16), a magnetic control knob (17) and a main switch (18).

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