Six-groove plate special for cell counting

Abstract

The utility model discloses a special six-groove plate for cell counting, and belongs to the technical field of biological cell counting. A special six-groove cell counting plate comprises a PC pressing plate which is arranged on the topmost portion of the device, and further comprises a double-faced adhesive tape layer arranged below the PC pressing plate, and a glass bottom plate arranged below the double-faced adhesive tape layer. Rectangular air holes which are linearly distributed at equal intervals are formed in the PC pressing plate, and semicircular air holes which are linearly distributed at equal intervals are formed in the PC pressing plate; a plurality of rectangular slots are formed in the double-sided adhesive layer; the semicircular air hole and the rectangular air hole are located over the two sides of the rectangular groove position respectively and communicate with the rectangular groove position. Compared with a traditional non-fixed attaching type counting plate, the cell counting plate has the advantages that sample plate abrasion caused in the attaching and assembling process of the cell counting plate can be reduced, meanwhile, operation is more convenient, and the problems that traditional sample preparation is complex in process and long in preparation time are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell counting, in particular to a six-slot special plate for cell counting. Background Art

[0002] With the development of biomedical experiments in society, biological cell experiments have also become an important part of the biomedical field. They include experiments such as cell culture, cell proliferation and migration, cell transfection and cell immunofluorescence. In these experiments, there is an operational process of counting cells.

[0003] At present, in most existing cell counting and observation processes, loose components are used and then sent to a microscope or electronic instrument for observation or counting. During this process, loosening and offset of the components will affect the observation results. In addition, the replacement of loose components is troublesome and complicated, which affects the user's operation. Based on this, the present utility model is proposed. Utility Model Content

[0004] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a six-slot cell counting plate that can overcome the above problems or at least partially solve the above problems.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A six-slot cell counting plate comprises a PC pressing plate, which is arranged at the top of the device. The plate further comprises: a double-sided adhesive layer provided below the PC pressing plate, and a glass bottom plate provided below the double-sided adhesive layer; rectangular air holes are provided on the PC pressing plate in an equidistant linear distribution, and semicircular air holes are provided on the PC pressing plate in an equidistant linear distribution; a plurality of rectangular slots are provided on the double-sided adhesive layer; the semicircular air holes and the rectangular air holes are respectively located directly above two sides of the rectangular slots, and the semicircular air holes and the rectangular air holes are respectively connected to the rectangular slots.

[0007] Preferably, the number of rectangular air holes and semicircular air holes opened on the PC pressing plate are both six.

[0008] Furthermore, the number of rectangular slots opened on the double-sided adhesive layer is , and each of the rectangular slots corresponds to a rectangular air hole and a semicircular air hole.

[0009] Preferably, the glass bottom plate is made of transparent material.

[0010] Preferably, the PC pressing plate, the double-sided adhesive layer and the glass bottom plate have the same shape.

[0011] Preferably, chamfers are provided around the PC pressing plate, the double-sided adhesive layer and the glass bottom plate.

[0012] Preferably, the long side of the rectangular slot is fifteen to twenty times the wide side of the rectangular air hole, and the wide side of the rectangular slot is slightly longer than the long side of the rectangular air hole.

[0013] Preferably, the long side of the rectangular air hole is equal to the diameter of the semicircular air hole.

[0014] Compared with the prior art, the present invention provides a six-slot cell counting plate with the following advantages:

[0015] 1. The six-slot cell counting plate can reduce the wear of the sample plate caused by the cell counting plate during the assembly process by fixing and bonding, thereby making the counting results more accurate, simplifying the early assembly preparation process, and saving time.

[0016] The parts not involved in this device are the same as the existing technology or can be implemented using existing technology. Compared with the traditional non-fixed bonding counting plate, the utility model can reduce the wear of the sample plate caused by the bonding and assembly of the cell counting plate, and at the same time make the operation more convenient, solving the problems of complex process and long preparation time of traditional sample preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a structural diagram of a six-slot cell counting plate proposed by the utility model;

[0018] Figure 2 This is a top view of the PC pressing plate in a six-slot cell counting plate proposed in the utility model;

[0019] Figure 3 This is a top view of the glass bottom plate in a six-slot cell counting plate proposed in the present invention;

[0020] Figure 4 This is a top view of the double-sided adhesive layer in a six-slot cell counting plate proposed in the utility model;

[0021] Figure 5 This is a side view of a six-slot cell counting plate proposed by the utility model.

[0022] In the figure: 1. PC pressing plate; 11. Rectangular air hole; 12. Semicircular air hole; 2. Double-sided adhesive layer; 21. Rectangular slot; 3. Glass bottom plate. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0024] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0025] Example 1:

[0026] Reference Figure 1-Figure 5 A six-slot cell counting plate includes a PC pressing plate 1, which is arranged at the top of the device, and also includes: a double-sided adhesive layer 2 is provided below the PC pressing plate 1, and a glass bottom plate 3 is provided below the double-sided adhesive layer 2; rectangular air holes 11 are provided on the PC pressing plate 1 and are distributed linearly at equal intervals, and semicircular air holes 12 are provided on the PC pressing plate 1 and are distributed linearly at equal intervals; a plurality of rectangular slots 21 are provided on the double-sided adhesive layer 2; the semicircular air holes 12 and the rectangular air holes 11 are respectively located directly above the two sides of the rectangular slots 21, and the semicircular air holes 12 and the rectangular air holes 11 are respectively connected to the rectangular slots 21.

[0027] The present invention is composed of a three-layer structure. The top layer of the three-layer structure is a PC pressing plate 1, which has rectangular air holes 11 and semicircular air holes 12. The middle layer is a double-sided adhesive layer 2 structure, which retains rectangular slots 21. The bottom layer is a glass bottom plate 3. The structure of the glass bottom plate 3 is double-sided transparent. The top PC pressing plate 1 and the bottom glass bottom plate 3 are bonded together by the double-sided adhesive layer 2, so that the sample can be injected and discharged through the rectangular air holes 11 and the semicircular air holes 12. While the double-sided adhesive layer 2 bonds the upper and lower layers, the reserved rectangular slots 21 also provide a slot thickness of 0.20 mm, which is convenient for sample placement and observation.

[0028] By bonding and fixing the top PC pressing plate 1 and the bottom glass base plate 3 with a double-sided adhesive layer 2, the test sample can be injected through the rectangular air holes 11 and the semicircular air holes 12, which is beneficial to the diffusion of the sample without generating air and affecting the test results. The rectangular air holes 11 and the semicircular air holes 12 make the sample more hydrophilic during injection, making the injection of the sample more convenient. The different air hole designs on both sides facilitate the injection of the sample on the one hand, and on the other hand, while retaining the air holes, it also reduces the sample loss caused by the shaking of the cell plate, which ultimately affects the test results.

[0029] The device is made of three layers of material, and is a fixed set of plates whether in use or not. When use is completed, one only needs to pinch the blank area of the device and slowly take it out, which reduces the operation process of the device and thus reduces the wear of the sample plate caused by the assembly process of the device.

[0030] Example 2:

[0031] Reference Figure 1-Figure 5 , which is basically the same as Example 1, and furthermore, the number of the rectangular air holes 11 and the semicircular air holes 12 opened on the PC pressing plate 1 is six, the number of the rectangular slots 21 opened on the double-sided adhesive layer 2 is six, each rectangular slot 21 corresponds to a rectangular air hole 11 and a semicircular air hole 12, the glass base plate 3 is made of transparent material, the PC pressing plate 1, the double-sided adhesive layer 2 and the glass base plate 3 have the same shape, the PC pressing plate 1, the double-sided adhesive layer 2 and the glass base plate 3 are chamfered around, the long side of the rectangular slot 21 is fifteen to twenty times the wide side of the rectangular air hole 11, the wide side of the rectangular slot 21 is slightly longer than the long side of the rectangular air hole 11, and the long side of the rectangular air hole 11 is equal to the diameter of the semicircular air hole 12.

[0032] Preferably, the long sides of the PC pressing plate 1, the double-sided adhesive layer 2, and the glass bottom plate 3 are all 96.00 mm and the wide sides are all 25.50 mm. The long side of the rectangular slot 21 is 17.50 mm and the wide side is 6.00 mm. The diameter of the semicircular air hole 12 is 5.60 mm. The long side of the rectangular air hole 11 is 5.60 mm and the wide side is 1.00 mm. The distance between two adjacent rectangular air holes 11 is 5.40 mm. The distance between two adjacent semicircular air holes 12 is 5.40 mm. The spacing between the rectangular air holes 11 and the semicircular air holes 12 is 13.71 mm. The distance between two adjacent rectangular slots 21 and adjacent slots is 5.00 mm.

[0033] When using, the user should first confirm whether the detection instrument is connected correctly and can be used normally, and then take out the device. Inject the sample to be tested into the rectangular slot 21 through the semicircular air hole 12. During the injection process, pay attention to the injection speed to avoid injecting too quickly and squeezing the test sample out of the rectangular slot 21. Then pinch the tail end of the cell counting plate to keep the PC pressure plate 1 facing upwards, and place the device steadily at the bottom of the cell counter to start the test. After use, pinch the tail end of the device and take it out steadily to prevent the test sample in the rectangular slot 21 from dripping. Then discharge the test sample inside the rectangular slot 21 through the rectangular air hole 11 and the semicircular air hole 12, and use medical alcohol to disinfect and clean it and then package it.

[0034] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the present invention can make equivalent replacements or changes based on the technical solution and utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A six-slot cell counting plate, comprising a PC pressing plate (1), wherein the PC pressing plate (1) is arranged at the top of the device, characterized in that: Also includes: A double-sided adhesive layer (2) is provided below the PC pressing plate (1), and a glass bottom plate (3) is provided below the double-sided adhesive layer (2); The PC pressing plate (1) is provided with rectangular air holes (11) distributed in an equidistant linear manner, and the PC pressing plate (1) is provided with semicircular air holes (12) distributed in an equidistant linear manner; The double-sided adhesive layer (2) is provided with a plurality of rectangular slots (21); The semicircular air holes (12) and the rectangular air holes (11) are respectively located directly above the two sides of the rectangular slot (21), and the semicircular air holes (12) and the rectangular air holes (11) are respectively connected to the rectangular slot (21).

2. A six-slot cell counting plate according to claim 1, characterized in that: The number of rectangular air holes (11) and semicircular air holes (12) provided on the PC pressing plate (1) is six.

3. A six-slot cell counting plate according to claim 2, characterized in that: The number of rectangular slots (21) provided on the double-sided adhesive layer (2) is six, and each rectangular slot (21) corresponds to a rectangular air hole (11) and a semicircular air hole (12).

4. A six-slot cell counting plate according to claim 1, characterized in that: The glass bottom plate (3) is made of transparent material.

5. A six-slot cell counting plate according to claim 1, characterized in that: The PC pressing plate (1), the double-sided adhesive layer (2) and the glass bottom plate (3) have the same shape.

6. A six-slot cell counting plate according to claim 1, characterized in that: The PC pressing plate (1), the double-sided adhesive layer (2) and the glass bottom plate (3) are provided with chamfers around their peripheries.

7. A six-slot cell counting plate according to claim 1, characterized in that: The long side of the rectangular slot (21) is 15 to 20 times the wide side of the rectangular air hole (11), and the wide side of the rectangular slot (21) is slightly longer than the long side of the rectangular air hole (11).

8. The six-slot cell counting plate according to claim 1, characterized in that: The long side of the rectangular air hole (11) is equal to the diameter of the semicircular air hole (12).

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