Purification device for biosurfactant rhamnolipid

Abstract

The utility model relates to the technical field of microbial fermentation, and discloses a biosurfactant rhamnolipid purification device which comprises a fermentation tank and an electric stirring rod installed at the top end of the fermentation tank in a penetrating mode, a feeding frame is installed at the upper end of the outer side of the fermentation tank in a penetrating mode, and a material collecting structure is installed on one side of the fermentation tank. The cutting component comprises a discharge pipe penetrating through the outer surface of the fermentation tank, one end of the discharge pipe is provided with an electromagnetic valve, one end of the electromagnetic valve is provided with a connecting pipe, the stirred raw materials are subjected to fermentation and liquid separation in the fermentation tank, a group of electromagnetic valves are opened, clear liquid is discharged into the vertical pipe and the J-shaped bent pipe through the discharge pipe, the electromagnetic valves and the connecting pipe, and after the electromagnetic valves are closed, the clear liquid is discharged into the vertical pipe and the J-shaped bent pipe. The air cylinder extends, the rubber plug moves downwards in the vertical pipe, the nanofiltration membrane filters out large impurities or particles in the clear liquid, and the filtered clear liquid enters the middle hole, is discharged into the liquid discharging pipe through the one-way valve B and is collected in the liquid tank.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial fermentation, in particular to a purification device for biosurfactant rhamnolipid. Background Art

[0002] Rhamnolipids are a class of natural surfactants composed of rhamnose and fatty acids. They are typically synthesized by certain bacteria (such as Pseudomonas, particularly Pseudomonas aeruginosa) during their growth. Rhamnolipids have applications in various fields, particularly in bio-cleaning, environmental protection, and medicine.

[0003] For example, a dedicated device for purifying a biosurfactant rhamnolipid, disclosed in publication number CN211677607U, comprises a main body, a control switch group disposed on the outer surface of the main body, the input end of the control switch group being electrically connected to the output end of an external power supply, three feeding tubes evenly mounted on the upper surface of the main body, the feeding tubes being connected to an external feeding mechanism, and a discharge tube disposed on the lower side surface of the main body. This dedicated device for purifying a biosurfactant rhamnolipid utilizes a plurality of staggered first and second clear liquid discharge tubes to discharge a corresponding amount of upper clear liquid by gravity based on the height of the clear liquid within the main body, thereby facilitating the removal of the clear liquid and adjusting its pH. Furthermore, an observation window and an illumination device allow for convenient observation of the clear liquid level, thereby facilitating the opening of a solenoid valve at the corresponding position for more convenient removal of the clear liquid. This device is simple to operate, improves work efficiency, and facilitates rapid purification.

[0004] The above patent proposes that the level of the clear liquid can be easily observed through an observation window and a lighting device. However, in actual use, light may affect the bacterial fatty acid synthesis, sugar metabolism and other biosynthetic pathways, indirectly affecting the production of rhamnolipids, resulting in reduced efficiency in the preparation of rhamnolipids and inconvenience in use.

[0005] Therefore, we proposed a purification device for biosurfactant rhamnolipid to solve the above problems. Utility Model Content

[0006] The purpose of the utility model is to provide a purification device for biosurfactant rhamnolipid, so as to solve the problem in the background art that light may affect the yield of rhamnolipid.

[0007] To achieve the above-mentioned object, the present invention provides the following technical solution: a device for purifying rhamnolipid, a biosurfactant, comprising a fermentation tank and an electric stirring rod installed through the top of the fermentation tank, a feed frame installed through the outer upper end of the fermentation tank, a material receiving structure installed on one side of the fermentation tank, the material receiving structure comprising a cutting member, a liquid transport member installed at one end of the cutting member, a filter member installed on one side of the liquid transport member, a material storage member installed at the top of one side of the liquid transport member, and the material storage member is arranged above the filter member;

[0008] The material opening component comprises a pipe installed through the outer surface of the fermentation tank, one end of the pipe is installed with a solenoid valve, and one end of the solenoid valve is installed with a connecting pipe.

[0009] The liquid transport component includes a vertical pipe and a J-bend installed at the bottom end of the vertical pipe. A groove is opened at the upper end of one side of the J-bend. A one-way valve A is installed inside the J-bend and at the lower end of the groove. A filling block is installed inside the J-bend and on the outer surface of the groove.

[0010] Preferably, a cylinder is installed through the top end of the vertical tube, the movable end of the cylinder is located inside the vertical tube, and a rubber stopper is installed on the movable end of the cylinder.

[0011] Preferably, the filter component includes a bent plate arranged inside the slot, an internal ring is installed inside the bent plate, and a nanofiltration membrane is installed inside the internal ring.

[0012] Preferably, the storage component includes a liquid tank and a tank cover threadedly connected to the top of the liquid tank, a T-shaped threaded tube is installed at the bottom end of the liquid tank, a center hole is opened through the middle end of the T-shaped threaded tube, a one-way valve B is installed inside the center hole, a drain pipe is installed at the bottom end of the liquid tank, the liquid outlet end of the one-way valve B is located inside the drain pipe, and a rubber ring is installed at the top of the J-bend pipe.

[0013] Preferably, the outer surface of the J-bend pipe is provided with connecting hole plates on both sides of the slot, and side plates are provided on both sides of the bend plate, and the middle ends of the side plates are threadedly connected with bolts.

[0014] Preferably, a rubber strip is installed between the side plate and the built-in ring, and one side of the rubber strip is in contact with the J-bend.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] 1. The utility model discloses a device for purifying rhamnolipid, a biosurfactant. The raw materials after stirring are fermented and separated inside a fermentation tank. A set of solenoid valves is opened. As the clear liquid inside the fermentation tank flows, a second set of solenoid valves can be opened to facilitate the extraction of the clear liquid. The fermentation tank and the material receiving structure are made of light-shielding materials to avoid affecting the yield of rhamnolipid.

[0017] 2. The utility model provides a purification device for a biosurfactant rhamnolipid. The clear liquid is discharged into the interior of the vertical pipe and the J-bend pipe through a discharge pipe, a solenoid valve and a connecting pipe. When the clear liquid inside the fermentation tank is completely discharged into the interior of the vertical pipe, the solenoid valve is closed, the cylinder is extended, the nanofiltration membrane filters out larger impurities or particulate matter in the clear liquid, and the liquid tank collects the liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the three-dimensional structure of the material cutting component and the liquid transport component of the present utility model;

[0020] Figure 3 This is a schematic diagram of the three-dimensional structure of the filter component of the present utility model;

[0021] Figure 4 This is a schematic diagram of the three-dimensional structure of the material storage component of the present utility model.

[0022] In the figure: 1. fermentation tank; 2. electric stirring rod; 3. feeding frame; 4. material receiving structure; 41. material opening component; 411. discharge pipe; 412. solenoid valve; 413. connecting pipe; 42. liquid transport component; 421. vertical pipe; 422. cylinder; 423. rubber plug; 424. J-bend pipe; 425. slot; 426. one-way valve A; 427. filling block; 428. connecting hole plate; 429. rubber ring; 43. filtering component; 431. bent plate; 432. built-in ring; 433. rubber strip; 434. side plate; 435. bolt; 436. nanofiltration membrane; 44. material storage component; 441. liquid tank; 442. tank cover; 443. T-shaped threaded pipe; 444. center hole; 445. one-way valve B; 446. discharge pipe. DETAILED DESCRIPTION

[0023] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] Example 1: Please refer to Figure 1-Figure 3A device for purifying rhamnolipid, a biosurfactant, comprises a fermentation tank 1 and an electric stirring rod 2 installed through the top of the fermentation tank 1, a feeding frame 3 is installed through the upper end of the outer side of the fermentation tank 1, a material receiving structure 4 is installed on one side of the fermentation tank 1, and the material receiving structure 4 comprises a cutting member 41, a liquid transport member 42 is installed at one end of the cutting member 41, a filtering member 43 is installed on one side of the liquid transport member 42, a storage member 44 is installed on the top of one side of the liquid transport member 42, and the storage member 44 is arranged above the filtering member 43.

[0025] The material opening component 41 includes a drain pipe 411 installed through the outer surface of the fermentation tank 1, a solenoid valve 412 is installed at one end of the drain pipe 411, and a connecting pipe 413 is installed at one end of the solenoid valve 412. There are multiple groups of drain pipes 411, solenoid valves 412 and connecting pipes 413.

[0026] The liquid transport component 42 includes a vertical pipe 421 and a J-bend pipe 424 installed at the bottom end of the vertical pipe 421. A groove 425 is provided at the upper end of one side of the J-bend pipe 424. A one-way valve A426 is installed inside the J-bend pipe 424 and at the lower end of the groove 425. A filling block 427 is installed inside the J-bend pipe 424 and on the outer surface of the groove 425. The clear liquid inside the cutting component 41 can be discharged into the interior of the vertical pipe 421 and the J-bend pipe 424.

[0027] A cylinder 422 is installed through the top of the vertical tube 421, and the movable end of the cylinder 422 is inside the vertical tube 421. A rubber plug 423 is installed on the movable end of the cylinder 422. The extension of the vertical tube 421 can push the rubber plug 423 downward, thereby pushing the clear liquid to flow.

[0028] The filter component 43 includes a bent plate 431 disposed inside the slot 425 . An internal ring 432 is installed inside the bent plate 431 . A nanofiltration membrane 436 is installed inside the internal ring 432 . The nanofiltration membrane 436 is used to filter the clear liquid.

[0029] The material storage component 44 includes a liquid tank 441 and a tank cover 442 threadedly connected to the top of the liquid tank 441. A T-shaped threaded tube 443 is installed at the bottom end of the liquid tank 441. A center hole 444 is penetrated through the middle end of the T-shaped threaded tube 443. A one-way valve B445 is installed inside the center hole 444. A drain pipe 446 is installed at the bottom end of the liquid tank 441. The liquid outlet end of the one-way valve B445 is located inside the drain pipe 446. A rubber ring 429 is installed at the top of the J-bend 424. The one-way valve B445 can maintain the one-way flow of the filtered clear liquid. The T-shaped threaded tube 443 is threadedly connected to the inside of the J-bend 424. The rubber ring 429 is provided to seal the T-shaped threaded tube 443 and the J-bend 424.

[0030] In this embodiment, the raw materials are discharged into the interior of the fermentation tank 1 through the feed frame 3, and the raw materials are stirred by the electric stirring rod 2. The stirred raw materials are fermented and separated in the interior of the fermentation tank 1. A set of electromagnetic valves 412 is opened, and the clear liquid is discharged into the interior of the vertical pipe 421 and the J-bend pipe 424 through the discharge pipe 411, the electromagnetic valve 412 and the connecting pipe 413. As the clear liquid in the fermentation tank 1 flows, the second set of electromagnetic valves 412 can be opened, and the clear liquid accumulates in the interior of the liquid transport component 42, which promotes the clear liquid to pass through the fermentation tank 1. The one-way valve A426 discharges into one side of the J-bend 424, and the clear liquid is filtered through the nanofiltration membrane 436 to remove larger impurities or particulate matter in the clear liquid. The filtered clear liquid enters the interior of the central hole 444 and is discharged into the interior of the discharge pipe 446 through the one-way valve B445. As the filtered clear liquid accumulates inside the discharge pipe 446, it is caused to overflow and is collected inside the liquid tank 441. The fermentation tank 1 and the material collection structure 4 use light-shielding materials to avoid affecting the production of rhamnolipids.

[0031] Example 2: This example is an improvement based on Example 1. For details, please refer to Figure 2-Figure 4 The outer surface of the J-bend 424 is provided with a connecting hole plate 428 on both sides of the slot 425, and side plates 434 are provided on both sides of the bent plate 431. The middle end of the side plate 434 is threadedly connected with a bolt 435, and the threaded end of the bolt 435 is threaded into the interior of the connecting hole plate 428 to install the filter component 43.

[0032] A rubber strip 433 is installed between the side plate 434 and the built-in ring 432. One side of the rubber strip 433 contacts the J-bend 424. When the filter component 43 is installed, the rubber strip 433 can seal the filter component 43 and the J-bend 424.

[0033] In this embodiment, when the clear liquid inside the fermentation tank 1 is completely discharged into the vertical pipe 421, the solenoid valve 412 is closed, the cylinder 422 is extended, and the rubber plug 423 moves downward inside the vertical pipe 421. The rubber plug 423 is used to push the clear liquid in the vertical pipe 421 and the J-bend pipe 424, so that the clear liquid completely passes through the nanofiltration membrane 436 to avoid waste of the clear liquid. After the clear liquid is collected, the storage component 44 can be rotated, and the T-shaped threaded tube 443 is rotated out from the top of one side of the J-bend pipe 424. The storage component 44 can be disassembled separately. The clear liquid is inside the liquid tank 441. The valve B445 prevents the filtered clear liquid from being discharged from the drain pipe 446 and the center hole 444 when the storage component 44 is disassembled. Afterwards, the bolt 435 can be rotated to pull the filter component 43 out from the inside of the slot 425. The operator can clean the impurities or particulate matter inside the J-bend 424, clean the nanofiltration membrane 436, insert the filter component 43 into the inside of the slot 425, and use the bolt 435 to thread it into the inside of the connecting hole plate 428. The rubber strip 433 is used to seal the side plate 434 and the J-bend 424 to prevent the filtered clear liquid from leaking.

[0034] Working principle: the raw materials after stirring are fermented and separated inside the fermentation tank 1, and a set of solenoid valves 412 are opened, and the clear liquid is discharged into the vertical pipe 421 and the J-bend 424 through the discharge pipe 411, the solenoid valve 412 and the connecting pipe 413. When the clear liquid inside the fermentation tank 1 is completely discharged into the vertical pipe 421, the solenoid valve 412 is closed, the cylinder 422 is extended, and the rubber plug 423 moves downward inside the vertical pipe 421, prompting the clear liquid to completely pass through the nanofiltration membrane 436, and filter out larger impurities or particulate matter in the clear liquid. The filtered clear liquid enters the interior of the center hole 444 and is discharged into the interior of the discharge pipe 446 through the one-way valve B445. As the filtered clear liquid accumulates inside the discharge pipe 446, the clear liquid is prompted to overflow and the liquid is collected inside the liquid tank 441.

[0035] The contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0036] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for purifying rhamnolipid as a biosurfactant, comprising a fermentation tank (1) and an electric stirring rod (2) installed through the top of the fermentation tank (1), a feed frame (3) installed through the upper end of the outer side of the fermentation tank (1), and a material receiving structure (4) installed on one side of the fermentation tank (1), characterized in that: The material receiving structure (4) comprises a material cutting component (41), a liquid transporting component (42) is installed at one end of the material cutting component (41), a filtering component (43) is installed at one side of the liquid transporting component (42), a material storage component (44) is installed at the top end of one side of the liquid transporting component (42), and the material storage component (44) is arranged above the filtering component (43); The material opening component (41) includes a pipe (411) installed through the outer surface of the fermentation tank (1), a solenoid valve (412) is installed at one end of the pipe (411), and a connecting pipe (413) is installed at one end of the solenoid valve (412); The liquid transport component (42) includes a vertical pipe (421) and a J-bend pipe (424) installed at the bottom end of the vertical pipe (421). A slot (425) is provided at the upper end of one side of the J-bend pipe (424). A one-way valve A (426) is installed inside the J-bend pipe (424) and at the lower end of the slot (425). A filling block (427) is installed inside the J-bend pipe (424) and on the outer surface of the slot (425).

2. The device for purifying a biosurfactant rhamnolipid according to claim 1, characterized in that: A cylinder (422) is installed through the top end of the vertical tube (421), the movable end of the cylinder (422) is located inside the vertical tube (421), and a rubber plug (423) is installed at the movable end of the cylinder (422).

3. The device for purifying a biosurfactant rhamnolipid according to claim 1, characterized in that: The filter component (43) includes a bent plate (431) arranged inside the slot (425), a built-in ring (432) is installed inside the bent plate (431), and a nanofiltration membrane (436) is installed inside the built-in ring (432).

4. The device for purifying rhamnolipid as a biosurfactant according to claim 3, characterized in that: The material storage member (44) comprises a liquid tank (441) and a tank cover (442) threadedly connected to the top of the liquid tank (441); a T-shaped threaded tube (443) is installed at the bottom of the liquid tank (441); a center hole (444) is provided through the middle of the T-shaped threaded tube (443); a one-way valve B (445) is installed inside the center hole (444); a liquid discharge pipe (446) is installed at the bottom of the liquid tank (441); the liquid outlet end of the one-way valve B (445) is located inside the liquid discharge pipe (446); and a rubber ring (429) is installed at the top of the J-bend pipe (424).

5. The device for purifying rhamnolipid as claimed in claim 4, characterized in that: The outer surface of the J-bend (424) and both sides of the slot (425) are equipped with connecting hole plates (428), and both sides of the bent plate (431) are equipped with side plates (434), and the middle ends of the side plates (434) are threadedly connected with bolts (435).

6. The device for purifying rhamnolipid as claimed in claim 5, characterized in that: A rubber strip (433) is installed between the side plate (434) and the built-in ring (432), and one side of the rubber strip (433) is in contact with the J-bend (424).

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