Separate-column separate-control tray device
By designing a split-controlled tray device in a DNA synthesizer, the separate operation of each synthesis column is achieved, which solves the needs of multiple batches of synthesis and reduces the cost of DNA synthesis.
Patent Information
- Application Number
- CN202420040324.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-01-08
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2034-01-08
AI Technical Summary
In existing DNA synthesizers, 96-well synthetic plates cannot meet the synthesis requirements of multiple batches and individualization, resulting in waste of raw materials and high synthesis costs.
A split-column control tray device is designed, and each row of synthetic columns is sealed separately by setting a storage cavity and a sealing structure under the synthetic plate to control liquid addition and liquid extraction operations according to the column.
Multi-batch and individualized DNA synthesis have been achieved, reducing raw material waste and synthesis costs.
Smart Images
Figure CN223197007U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biotechnology, in particular to a separate column and separate control tray device. Background Art
[0002] DNA synthesis, a key foundational technology in synthetic biology, is as important as sequencing technology is to genomics and is widely used in the life sciences. DNA synthesis principles primarily include chemical and biological methods, with chemical methods being the most mature and widely used. DNA synthesizers are the core equipment for DNA synthesis.
[0003] DNA synthesizers commonly use 96-well plates, with wells arranged in a linear pattern of 8 x 12, each containing a synthesis column. During operation, the DNA synthesizer injects reagents into each column, and after the reaction is complete, the remaining liquid is extracted using negative pressure. The 96 columns share a closed chamber, allowing for simultaneous extraction of the post-reaction liquid.
[0004] Since the samples come from multiple customers, each customer has different needs, requires different reagents and dosages, and has different reaction times, the simultaneous extraction of 96 synthesis columns cannot meet the requirements of multi-batch, individualized synthesis, resulting in waste of raw materials and affecting the efficiency of synthesis, thus leading to high costs for DNA synthesis. Utility Model Content
[0005] In response to the above-mentioned shortcomings of the prior art, the utility model provides a column-by-column and control-by-column tray device, which can realize separate synthesis operations for each column or row of synthesis columns on the synthesis plate, realize column-by-column or row-by-row control, meet the requirements of multi-batch and individualized synthesis, avoid waste of raw materials, improve synthesis efficiency, and thus reduce the cost of DNA synthesis.
[0006] The utility model provides a column-by-column and control-by-column pallet device, comprising a composite plate, on which a plurality of composite columns are arranged in a matrix to form N columns of composite columns, and further comprising:
[0007] A tray is disposed below the composite board, the tray being provided with N accommodating cavities, one accommodating cavity being used to accommodate a row of composite columns, one side of the accommodating cavity being provided with an interface for communicating with the interior of the accommodating cavity, the N accommodating cavities corresponding to the positions of the N rows of composite columns, and one end of each row of composite columns being located in a corresponding accommodating cavity;
[0008] The sealing structure is arranged between the tray and the synthetic board, and is used to form a sealed connection between the tray and the synthetic board, so that each accommodating cavity forms a sealed chamber.
[0009] Preferably, an opening is provided at the top of the accommodating cavity, and the four sides of the opening are sealedly connected to the synthetic board.
[0010] Preferably, the sealing structure includes a sealing gasket, which is arranged between the pallet and the synthetic board. The sealing gasket is provided with N avoidance holes, and the shape and position of the avoidance holes match the shape and position of the opening.
[0011] Preferably, a limiting groove is provided on the tray, the sealing gasket is arranged in the limiting groove, and the bottom of the synthetic board is also arranged in the limiting groove.
[0012] Preferably, the N columns of synthetic columns are replaced by N rows of synthetic columns.
[0013] Preferably, a plurality of pressing blocks are provided on the tray, the pressing blocks are detachably connected to the tray, and the pressing blocks are used to press the synthetic board.
[0014] Preferably, one end of the pressing block is connected to a rotating rod, which is rotatably connected to the pressing block. The other end of the pressing block is arranged on a side of the synthetic board away from the pallet, and the rotating rod is threadedly connected to the pallet.
[0015] Preferably, the pallets are provided in plurality and the plurality of pallets are connected as one body, and the synthetic boards are provided in plurality and the plurality of synthetic boards are respectively arranged on corresponding pallets.
[0016] Compared with the prior art, the utility model provides a separate column and separate control tray device, which has the following beneficial effects:
[0017] The utility model provides a receiving cavity on the tray below the synthetic board, arranges each column of synthetic columns in the corresponding receiving cavity, and seals the receiving cavity through a sealing structure. An interface for adding and extracting liquid is provided on the receiving cavity. When in use, the individual synthesis operation of each column of synthetic columns on the synthetic board can be realized through the liquid adding and extracting device, so that a synthetic board can be controlled separately by column, providing the option of multi-type and multi-batch synthesis for production, and reducing costs. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0019] Figure 2 It is a three-dimensional diagram of the utility model;
[0020] Figure 3 It is an exploded view of the overall structure of the utility model.
[0021] Description of reference numerals:
[0022] 1. Tray; 2. Sealing gasket; 3. Synthetic column; 4. Waste liquid box; 5. Interface; 6. Air pipe; 7. Pressure block; 8. Gasket; 9. Fixing bolt; 10. Pressure block bolt; 11. Synthetic board. DETAILED DESCRIPTION
[0023] The following is combined with Figures 1 to 3 , the specific implementation methods of the present utility model are described in detail, but it should be understood that the protection scope of the present utility model is not limited by the specific implementation methods.
[0024] In the description of the present invention, the terms "center", "upper", "lower", "left", "right", "inner", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or components referred to must have a specific direction, be constructed and operated in a specific direction, and should not be understood as limiting the present invention. Unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, an indirect connection through an intermediate medium, or it can be internal communication between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0025] In addition, in the description of this application, "plurality" means two or more than two. The terms "first" and "second" are used for descriptive purposes only and should not be understood to imply or suggest relative importance or implicitly indicate the number of technical features indicated. Therefore, features specified as "first" or "second" may explicitly or implicitly include one or more of the features.
[0026] Example 1
[0027] The present embodiment provides a column-by-column and control-by-control pallet device, comprising a composite plate 11 on which a plurality of composite columns 3 are arranged in a matrix to form N columns of composite columns 3, and further comprising a pallet 1 and a sealing structure.
[0028] The tray 1 is arranged below the synthetic board 11. N accommodating cavities are provided on the tray 1. One accommodating cavity is used to accommodate a column of synthetic columns 3. An interface 5 for connecting to the interior of the accommodating cavity is provided on one side of the accommodating cavity. The N accommodating cavities correspond to the positions of the N columns of synthetic columns 3. One end of each column of synthetic columns 3 is located in a corresponding accommodating cavity. A sealing structure is provided between the tray 1 and the synthetic board 11, for forming a sealed connection between the tray 1 and the synthetic board 11, so that each accommodating cavity forms a sealed chamber.
[0029] The spacing between the tray 1's cavities, the row-to-row spacing of the synthesis columns 3 on the synthesis board 11, and the row-to-row spacing of the needles on the synthesizer's injection needle plate are all identical. After the tray 1 and synthesis board 11 are assembled and pressed together using a sealing structure, the injection needles inject liquid. At this point, each column of the tray 1 and synthesis board 11 forms a separate sealed space. A vacuum device allows for independent liquid extraction from each column of the synthesis board 11, enabling column-by-column control of injection and extraction within a single synthesis board 11.
[0030] In this embodiment, an opening is provided at the top of the accommodating cavity, and the four sides of the opening are sealed and connected to the synthetic board 11 .
[0031] The sealing structure includes a gasket 2 disposed between the tray 1 and the composite board 11. The gasket 2 is provided with N escape holes, the shape and position of which match the shape and position of the opening. The sealing interval of the gasket 2 is consistent with the interval of the cavity of the tray 1.
[0032] The tray 1, the sealing gasket 2 and the synthetic board 11 are installed in order from bottom to top. Since the spacing between the three parts forming the cavity is the same, the cavities isolated from each other are realized in columns.
[0033] In this embodiment, the tray 1 has 12 sealed cavities, each sealed cavity corresponds to the grid cavity of the sealing gasket 2. After installation, the sealing gasket 2 is installed exactly on the table top of the sealed cavity of the tray 1. The table top of the sealed cavity forms a limiting groove, and the width of the sealing gasket 2 is consistent with the width of the limiting groove.
[0034] Tray 1 has 12 sealed cavities, each corresponding to a column of composite board 11. Composite board 11 has 96 composite columns 3, arranged in rows of 12 and columns of 8. The rows and columns function identically and are interchangeable. Each sealed cavity in tray 1 corresponds to eight composite columns 3 on composite board 11.
[0035] After the pallet 1, sealing gasket 2 and synthetic board 11 are installed in order from bottom to top, the sealing gasket 2 is exactly located between the table surface forming the cavity of the pallet 1 and the distance between the two rows of synthetic columns 3 of the synthetic board 11. After pressurization, the pallet 1 and the synthetic board 11 can be kept sealed.
[0036] How to use this embodiment
[0037] In the working position, the tray 1, the sealing gasket 2 and the synthetic board 11 are assembled together and pressed tightly, and the injection needles on the injection needle plate correspond one-to-one to the synthetic columns 3 on the synthetic board 11. At this time, the tray 1 and each column of the synthetic board 11 form a separate sealed space, and the vacuum device can realize independent extraction of liquid from the 8 synthetic columns 3 in each column of the synthetic board 11, so that the synthetic columns 3 on a piece of synthetic board 11 can be synthesized by column.
[0038] Example 2
[0039] The separate column and separate control pallet device provided in this embodiment is provided with a plurality of pressing blocks 7 on the pallet 1 in order to further achieve sealing of the pallet and the synthetic board while facilitating adjustment of the synthetic board. The pressing blocks 7 are detachably connected to the pallet 1 and are used to press the synthetic board 11.
[0040] When in use, the composite board 11 and the tray 1 are clamped by rotating the pressing block 7. During operation, the rows and columns of the composite boards 11 can be easily adjusted by rotating the pressing block 7, which facilitates the selection and replacement of multi-type and multi-batch synthesis.
[0041] Specifically, one end of the pressing block 7 is connected to a rotating rod, which is rotatably connected to the pressing block 7. The other end of the pressing block 7 is arranged on the side of the composite board 11 facing away from the pallet 1, and the rotating rod is threadedly connected to the pallet 1. In this embodiment, one end of the rotating rod is fixedly connected to the pressing block bolt 10, and the pallet 1 has a threaded hole, and the pressing block bolt 10 is threadedly connected to the threaded hole.
[0042] In order to provide more types and batch synthesis options, multiple pallets 1 are provided, and the multiple pallets 1 are connected as one body; multiple synthesis boards 11 are provided, and the multiple synthesis boards 11 are respectively arranged on corresponding pallets 1.
[0043] The interface 5 at the bottom of the tray 1 for extracting liquid is a threaded interface, which is used to connect to the air pipe 6. Each tray 1 has 12 cavities, and each cavity has a threaded interface. A waste liquid box 4 is fixed to one side of the tray 1 by a fixing bolt 9. The extracted liquid can be temporarily stored in the waste liquid box 4. A gasket 8 is provided between the fixing bolt 9 and the tray 1.
[0044] The above disclosure is only a preferred specific embodiment of the present invention, but the embodiments of the present invention are not limited thereto, and any changes that can be conceived by those skilled in the art should fall within the scope of protection of the present invention.
Claims
1. A column-by-column and control-by-column pallet device, comprising a composite plate (11), on which a plurality of composite columns (3) are arranged in a matrix to form N columns of composite columns (3), characterized in that: Also includes: A tray (1) is arranged below the synthetic board (11), and the tray (1) is provided with N accommodating cavities, one accommodating cavity being used to accommodate a row of synthetic columns (3), and an interface for communicating with the interior of the accommodating cavity is provided on one side of the accommodating cavity, the N accommodating cavities corresponding to the positions of the N rows of synthetic columns (3), and one end of each row of synthetic columns (3) is located in a corresponding accommodating cavity; A sealing structure is provided between the tray (1) and the synthetic board (11) and is used to form a sealed connection between the tray (1) and the synthetic board (11), so that each accommodating cavity forms a sealed chamber.
2. A tray device with separate columns and separate controls according to claim 1, characterized in that: An opening is provided at the top of the accommodating cavity, and the four sides of the opening are sealed and connected to the synthetic board (11).
3. A tray device with separate columns and separate controls as claimed in claim 2, characterized in that: The sealing structure comprises a sealing gasket (2), which is arranged between the pallet (1) and the synthetic board (11). The sealing gasket (2) is provided with N avoidance holes, and the shape and position of the avoidance holes match the shape and position of the opening.
4. A tray device with separate columns and separate controls as claimed in claim 3, characterized in that: A limiting groove is provided on the tray (1), the sealing gasket (2) is arranged in the limiting groove, and the bottom of the synthetic board (11) is also arranged in the limiting groove.
5. The tray device with separate columns and separate controls according to claim 1, characterized in that: Replace N columns of composite columns (3) with N rows of composite columns (3).
6. The tray device with separate columns and separate controls according to claim 1, characterized in that: A plurality of pressing blocks (7) are provided on the tray (1), the pressing blocks (7) are detachably connected to the tray (1), and the pressing blocks (7) are used to limit the position of the synthetic board (11).
7. The tray device with separate columns and separate controls according to claim 6, characterized in that: One end of the pressing block (7) is connected to a rotating rod, and the rotating rod is rotatably connected to the pressing block (7). The other end of the pressing block (7) is arranged on a side of the synthetic board (11) away from the tray (1), and the rotating rod is threadedly connected to the tray (1).
8. The tray device with separate columns and separate controls according to claim 1, characterized in that: The trays (1) are provided in plurality, and the plurality of trays (1) are connected as one body; the synthetic boards (11) are provided in plurality, and the plurality of synthetic boards (11) are respectively provided on corresponding trays (1).