MRNA (messenger ribonucleic acid) purification device capable of being used by circulating continuous flow

Abstract

The utility model relates to the technical field of purification devices, and discloses an mRNA (messenger ribonucleic acid) purification device capable of being used by circulating continuous flow, which comprises a fixed frame and a control panel, the control panel is fixedly mounted on the side part of the fixed frame, a collecting barrel is mounted in the middle of the fixed frame, a material pumping opening is formed in the side part of the collecting barrel, and the material pumping opening is communicated with the control panel. A protection assembly is arranged in the middle of the collecting barrel, a discharging pipe is connected to the bottom of the collecting barrel, a discharging pump is installed on the side portion of the discharging pipe, a treatment barrel is connected to the side portion of the discharging pump, and a treatment assembly is arranged in the middle of the treatment barrel. The mRNA purification device is simple in structure, convenient to operate, capable of reducing manual operation errors and improving the stability of the purification process, convenient to replace and maintain due to the arrangement of the collecting barrel and the treatment barrel, capable of prolonging the service life of the device, and capable of protecting mRNA from being damaged in the purification process and keeping the biological activity of the mRNA due to the arrangement of the protection assembly in the collecting barrel.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices, in particular to an mRNA purification device that can be used in a cyclic continuous flow. Background Art

[0002] With the development of biotechnology, the demand for automated and integrated equipment is growing. These devices can reduce human intervention and improve the accuracy and repeatability of the production process. Existing mRNA purification devices may have problems such as complex operation, low purification efficiency, and inability to operate continuously. Some devices may not have the ability to be recycled, resulting in waste of raw materials and increased operating costs. Market and clinical needs have driven the development of efficient, continuous, and recyclable mRNA purification devices to adapt to large-scale production and high-quality requirements. The innovation of the recyclable continuous flow mRNA purification device lies in its design that enables continuous sample processing, reduces downtime, and improves purification efficiency. The modular design of the device facilitates maintenance and upgrades to adapt to different purification needs. The integration of automated control systems makes operation easier and reduces human error.

[0003] Traditional purification methods may require a lot of manual operations, which not only increases the difficulty of operation but also increases the possibility of errors. Therefore, they do not meet existing needs. To this end, we propose an mRNA purification device that can be used in a circular continuous flow. Utility Model Content

[0004] The utility model provides an mRNA purification device that can be used in a cyclic continuous flow, which has the beneficial effect of improving purification efficiency and solves the problem mentioned in the above background technology that the traditional purification method may require a lot of manual operation, which not only increases the difficulty of operation but also increases the possibility of error.

[0005] The utility model provides the following technical solution: an mRNA purification device that can be used in a circulating continuous flow, comprising a fixing frame and a control board, wherein the control board is fixedly installed on the side of the fixing frame, a collecting bucket is installed in the middle of the fixing frame, a material extraction port is opened on the side of the collecting bucket, a protective component is provided in the middle of the collecting bucket, a discharge pipe is connected to the bottom of the collecting bucket, a discharge pump is installed on the side of the discharge pipe, a treatment bucket is connected to the side of the discharge pump, and a treatment component is provided in the middle of the treatment bucket.

[0006] As an optional solution for an mRNA purification device that can be used in a circulating continuous flow according to the utility model, the processing barrel is installed in the middle of the mounting frame, and the mounting frame and the bottom of the fixed frame are both equipped with a rotary wheel. The side of the processing barrel is connected to a connecting pipe, and the side of the connecting pipe is equipped with a discharge pump, and the side of the discharge pump is provided with a discharge port.

[0007] As an optional solution for the mRNA purification device that can be used in a circulating continuous flow according to the present invention, the processing component includes a mounting groove opened in the middle of the processing barrel, a mounting frame is installed in the middle of the mounting groove, a flow tube is inserted in the middle of the mounting frame, and the flow tube is inserted in the middle of the processing groove.

[0008] As an optional solution for the mRNA purification device that can be used in a circulating continuous flow according to the present invention, the processing tank is arranged at the bottom of the installation frame, a motor is installed in the middle of the installation frame, a transmission column is installed on the side of the motor, a processing tube is arranged on the side of the transmission column, and the processing tube is inserted into the middle of the processing tank.

[0009] As an optional solution for the mRNA purification device that can be used in a circulating continuous flow according to the utility model, wherein: the side of the processing tube is connected to a fixed tube, the fixed tube is connected to the feed pump, the side of the transmission column is installed with a stirring blade, and the bottom of the processing tank is installed with a feed pump.

[0010] As an optional solution for an mRNA purification device that can be used in a circulating continuous flow according to the utility model, the protection component includes a collection tank opened in the middle of the collection barrel, an extrusion plate is installed in the middle of the collection tank, a slider is installed on the side of the extrusion plate through a connecting rod, a slide groove is opened on the side of the collection barrel, the slider slides in the slide groove, and an extrusion block is fixedly installed on the bottom of the extrusion plate.

[0011] As an optional solution for the mRNA purification device that can be used in a circular continuous flow according to the utility model, wherein: a rotating shaft is installed in the middle of the extrusion block, a rotating rod is hinged to the side of the rotating shaft, a connecting block is hinged to the side of the rotating rod through the installation shaft, an elastic rod is fixedly connected to the side of the connecting block, and a telescopic rod is fixedly connected to the bottom of the extrusion block.

[0012] As an optional solution for the mRNA purification device that can be used in a circulating continuous flow according to the utility model, wherein: the side of the telescopic rod is sleeved with an extrusion spring, the bottom of the telescopic rod is installed with a fixed block, the middle of the fixed block is hinged with a rotating rod through a rotating shaft, the rotating rod is hinged to the installation shaft, and a reinforcement plate is fixedly installed at the bottom of the fixed block.

[0013] The utility model has the following beneficial effects:

[0014] 1. This mRNA purification device that can be used in a cyclic continuous flow can achieve automated control of the purification process through the provision of a fixed frame and a control panel, reduce manual operation errors, and improve the stability of the purification process. The provision of a collection barrel and a processing barrel facilitates replacement and maintenance, thereby increasing the service life of the device. The provision of a protective component in the collection barrel can protect the mRNA from damage during the purification process and maintain its biological activity. The provision of a processing component, including a flow tube and a processing tank, can effectively separate and purify mRNA and improve the purification quality. The provision of a motor and a transmission column, in conjunction with the use of a stirring blade, can accurately control the flow and mixing of the solution, thereby optimizing the purification effect.

[0015] 2. This mRNA purification device that can be used in a circular continuous flow has a slider and a chute, which makes the operation of the extrusion plate more flexible and convenient, and facilitates the adjustment of the pressure during the purification process. The extrusion spring and the telescopic rod provide elastic adjustment functions to adapt to different purification requirements and material characteristics. The reinforcement plate increases the stability of the device, ensuring that it will not move or be damaged accidentally during the purification process. The coordinated use of the device can clean and maintain the equipment, which helps to maintain the long-term stable operation of the equipment. The closed system design and appropriate pumping system reduce the risk of cross contamination, thereby ensuring the quality of the purified product. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model.

[0017] Figure 2 It is a schematic diagram of the three-dimensional side structure of the utility model.

[0018] Figure 3 This is a schematic diagram of the processing component structure of the present utility model.

[0019] Figure 4 This is a schematic diagram of the protection component structure of the present utility model.

[0020] In the figure: 110, rotating wheel; 120, fixing frame; 130, discharge pipe; 140, control panel; 150, collecting barrel; 160, extraction port; 170, discharge pump; 180, processing barrel; 190, discharge pump; 200, connecting pipe; 210, discharge port; 220, mounting frame; 230, chute; 240, slider; 250, connecting rod; 260, extrusion plate; 270, extrusion block; 280, rotating shaft; 290, rotating rod; 300, telescopic rod; 310, extrusion Spring; 320, fixed block; 330, rotating shaft; 340, rotating rod; 350, connecting block; 360, mounting shaft; 370, elastic rod; 380, reinforcing plate; 390, collecting tank; 400, mounting tank; 410, mounting frame; 420, circulation pipe; 430, processing tank; 440, motor; 450, transmission column; 460, fixed pipe; 470, processing pipe; 480, stirring blade; 490, suction pump; 500, processing component; 510, protection component. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0022] Example 1: This example aims to solve the problem that traditional purification methods may require a lot of manual operations, which not only increases the difficulty of operation but also increases the possibility of error. Figures 1-4 A mRNA purification device that can be used in a circulating continuous flow includes a fixing frame 120 and a control board 140. The control board 140 is fixedly installed on the side of the fixing frame 120, a collecting barrel 150 is installed in the middle of the fixing frame 120, a material extraction port 160 is opened on the side of the collecting barrel 150, a protection component 510 is provided in the middle of the collecting barrel 150, a discharge pipe 130 is connected to the bottom of the collecting barrel 150, a discharge pump 170 is installed on the side of the discharge pipe 130, a processing barrel 180 is connected to the side of the discharge pump 170, and a processing component 500 is provided in the middle of the processing barrel 180.

[0023] The processing barrel 180 is installed in the middle of the mounting frame 220, and the mounting frame 220 and the bottom of the fixed frame 120 are both installed with a rotary wheel 110. The side of the processing barrel 180 is connected to a connecting pipe 200, and the side of the connecting pipe 200 is installed with a discharge pump 190. The side of the discharge pump 190 is provided with a discharge port 210. The processing component 500 includes a mounting groove 400 opened in the middle of the processing barrel 180, and a mounting frame 410 is installed in the middle of the mounting groove 400. A circulation pipe 420 is inserted in the middle of the mounting frame 410, and the circulation pipe 420 is inserted in the middle of the processing groove 430.

[0024] When the equipment needs to be operated, the transmission column 450 is driven by the motor 440. The transmission column 450 is connected to the processing tube 470 and the stirring blade 480. The stirring blade 480 is used to stir the mixture in the processing tank 430 to ensure uniform treatment. The processing tube 470 can be used for further fluid transmission or as a reaction container. The fixed tube 460 on the side of the processing tube 470 is connected to the discharge pump 190, and the discharge pump 190 puts the chemicals into the processing tank 430 through the discharge port 210, and then continues to be processed in the collection barrel 150, thereby processing the items in the processing tank 430.

[0025] The rotating wheel 110 at the bottom of the mounting frame 220 and the fixing frame 120 allows the processing barrel 180 to be moved, which is convenient for operation and maintenance. The extrusion plate 260 slides in the slide groove 230 through the slider 240. The design of the extrusion block 270 and the rotating shaft 280 allows the extrusion plate 260 to move in the collection tank 390, thereby exerting pressure on the mixture, which helps to separate and purify mRNA. The design of the elastic rod 370 and the telescopic rod 300 provides adjustable extrusion force. The extrusion spring 310 and the reinforcement plate 380 ensure the stability and safety of the operation. After a series of processing steps, the purified items flow into the collection tank 390 from the discharge pipe 130. When the items in the collection tank 390 reach a certain amount, the processed items can be discharged through the extraction port 160, or collected through a specific outlet for subsequent experiments or production processes.

[0026] In this embodiment: through the provision of the fixing frame 120 and the control panel 140, automated control of the purification process can be achieved, manual operation errors can be reduced, and the stability of the purification process can be improved; through the provision of the collecting barrel 150 and the processing barrel 180, replacement and maintenance are facilitated, thereby increasing the service life of the device; through the provision of the protective component 510 in the collecting barrel 150, the mRNA can be protected from damage during the purification process and its biological activity can be maintained; through the provision of the processing component 500, including the flow tube 420 and the processing tank 430, the mRNA can be effectively separated and purified, thereby improving the purification quality; through the provision of the motor 440 and the transmission column 450, in conjunction with the use of the stirring blade 480, the flow and mixing of the solution can be precisely controlled, thereby optimizing the purification effect.

[0027] Example 2: This example aims to solve the problem that traditional mRNA purification methods usually involve multiple steps, which often need to be repeated many times, are cumbersome to operate, and are prone to sample loss and contamination. This example is an improvement made on the basis of Example 1. For details, please refer to Figures 1-4 The processing tank 430 is set at the bottom of the installation frame 410, a motor 440 is installed in the middle of the installation frame 410, a transmission column 450 is installed on the side of the motor 440, a processing tube 470 is set on the side of the transmission column 450, and the processing tube 470 is inserted in the middle of the processing tank 430.

[0028] The side of the processing tube 470 is connected to a fixed tube 460, the fixed tube 460 is connected to the discharge pump 190, the side of the transmission column 450 is installed with a stirring blade 480, the bottom of the processing tank 430 is installed with a suction pump 490, the protection component 510 includes a collection tank 390 opened in the middle of the collecting barrel 150, the middle of the collecting tank 390 is installed with an extrusion plate 260, the side of the extrusion plate 260 is installed with a slider 240 through a connecting rod 250, the side of the collecting barrel 150 is opened with a slide groove 230, the slider 240 slides in the slide groove 230, and the bottom of the extrusion plate 260 is fixedly installed with an extrusion block 270.

[0029] A rotating shaft 280 is installed in the middle of the extrusion block 270, and a rotating rod 290 is hinged on the side of the rotating shaft 280. The side of the rotating rod 290 is hinged to a connecting block 350 through an installation shaft 360. The side of the connecting block 350 is fixedly connected to an elastic rod 370. The bottom of the extrusion block 270 is fixedly connected to a telescopic rod 300. The side of the telescopic rod 300 is sleeved with an extrusion spring 310. A fixed block 320 is installed at the bottom of the telescopic rod 300. The middle of the fixed block 320 is hinged to a rotating rod 340 through a rotating shaft 330. The rotating rod 340 is hinged to the installation shaft 360. A reinforcing plate 380 is fixedly installed on the bottom of the fixed block 320.

[0030] In this embodiment, the slider 240 and the chute 230 are provided to make the operation of the extrusion plate 260 more flexible and convenient, and facilitate the adjustment of the pressure during the purification process. The extrusion spring 310 and the telescopic rod 300 provide an elastic adjustment function to adapt to different purification requirements and material properties. The reinforcement plate 380 is provided to increase the stability of the device, ensuring that no accidental movement or damage occurs during the purification process. The coordinated use of the device allows for cleaning and maintenance of the equipment, helping to maintain long-term stable operation of the equipment. The closed system design and the appropriate pumping system reduce the risk of cross contamination, thereby ensuring the quality of the purified product.

[0031] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0032] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. An mRNA purification device capable of continuous circulation, comprising a fixing frame (120) and a control panel (140), characterized in that: The control panel (140) is fixedly mounted on the side of the fixing frame (120), a collecting barrel (150) is mounted in the middle of the fixing frame (120), a material extraction port (160) is provided on the side of the collecting barrel (150), a protective component (510) is arranged in the middle of the collecting barrel (150), a discharge pipe (130) is connected to the bottom of the collecting barrel (150), a discharge pump (170) is mounted on the side of the discharge pipe (130), a treatment barrel (180) is connected to the side of the discharge pump (170), and a treatment component (500) is arranged in the middle of the treatment barrel (180).

2. The mRNA purification device capable of continuous circulation according to claim 1, characterized in that: The processing barrel (180) is installed in the middle of the mounting frame (220), and the bottom of the mounting frame (220) and the fixing frame (120) are both installed with a rotating wheel (110). The side of the processing barrel (180) is connected to a connecting pipe (200), and the side of the connecting pipe (200) is installed with a discharge pump (190), and the side of the discharge pump (190) is provided with a discharge port (210).

3. The mRNA purification device capable of continuous circulation according to claim 2, characterized in that: The processing assembly (500) includes a mounting groove (400) opened in the middle of the processing barrel (180), a mounting frame (410) is installed in the middle of the mounting groove (400), a flow tube (420) is plugged into the middle of the mounting frame (410), and the flow tube (420) is plugged into the middle of the processing groove (430).

4. The mRNA purification device capable of continuous circulation according to claim 3, characterized in that: The processing tank (430) is arranged at the bottom of the installation frame (410), a motor (440) is installed in the middle of the installation frame (410), a transmission column (450) is installed on the side of the motor (440), a processing tube (470) is arranged on the side of the transmission column (450), and the processing tube (470) is plugged into the middle of the processing tank (430).

5. The mRNA purification device capable of continuous circulation according to claim 4, characterized in that: The side of the processing pipe (470) is connected to a fixed pipe (460), and the fixed pipe (460) is connected to the feed pump (190). The side of the transmission column (450) is installed with a stirring blade (480), and the bottom of the processing tank (430) is installed with a pumping pump (490).

6. The mRNA purification device capable of continuous circulation according to claim 1, characterized in that: The protection component (510) includes a collection trough (390) opened in the middle of the collection barrel (150), an extrusion plate (260) is installed in the middle of the collection trough (390), a slider (240) is installed on the side of the extrusion plate (260) through a connecting rod (250), a sliding groove (230) is opened on the side of the collection barrel (150), the slider (240) slides in the sliding groove (230), and an extrusion block (270) is fixedly installed on the bottom of the extrusion plate (260).

7. The mRNA purification device capable of continuous circulation according to claim 6, characterized in that: A rotating shaft (280) is installed in the middle of the extrusion block (270), a rotating rod (290) is hinged to the side of the rotating shaft (280), a connecting block (350) is hinged to the side of the rotating rod (290) through an installation shaft (360), an elastic rod (370) is fixedly connected to the side of the connecting block (350), and a telescopic rod (300) is fixedly connected to the bottom of the extrusion block (270).

8. The mRNA purification device capable of continuous circulation according to claim 7, characterized in that: The side of the telescopic rod (300) is sleeved with an extrusion spring (310), the bottom of the telescopic rod (300) is installed with a fixed block (320), the middle of the fixed block (320) is hinged with a rotating rod (340) through a rotating shaft (330), the rotating rod (340) is hinged with the installation shaft (360), and the bottom of the fixed block (320) is fixedly installed with a reinforcing plate (380).

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