Intelligent control system for cell culture
Through multi-level monitoring and intelligent control systems, precise control of the environment in the cell culture tank is achieved, which solves the problem of single control parameters of the cell culture tank, and improves the success rate and automation of cell culture.
Patent Information
- Application Number
- CN202421333911.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-12
- Publication Date
- 2025-08-08
- Estimated Expiration
- 2034-06-12
AI Technical Summary
The existing cell culture tank control parameters are relatively single, which makes it impossible to adjust the environment to the standard state in a timely manner when starting a new batch of cell culture or in an emergency.
An intelligent control system for cell culture is designed, including multiple monitoring modules, logical judgment modules, first control modules and second control modules. Through multi-level monitoring and intelligent judgment, automatic regulation of the environment in the cell culture tank is realized. The first control module is used to control each operation module separately, and the second control module controls multiple operation modules at the same time.
It improves the quality and efficiency of cell culture, reduces artificial operation errors, improves the control accuracy and automation level of cell culture tanks, and ensures the stability and rapid response of the culture environment.
Smart Images
Figure CN223201858U_ABST
Abstract
Description
Technical Field
[0001] The present disclosure relates to the field of intelligent control technology, and in particular to a cell culture intelligent control system. Background Art
[0002] The function of a cell culture tank is to provide an optimized physical and chemical environment for cell metabolism, enabling faster and better cell growth and producing more cell products. Using a cell culture tank makes it easy to observe and assess the progress of cell culture, especially in the laboratory, allowing for timely identification of problems and adjustments. Accurate control of various parameters during the culture process is key to ensuring stable cell growth.
[0003] However, the current control parameters of cell culture tanks are relatively simple, resulting in the inability to adjust them in a timely manner, for example, when starting a new batch of cell culture or quickly restoring the environment in the entire cell culture tank to a standard state in an emergency.
[0004] Therefore, an automatic control scheme for cell culture tanks is proposed to solve the above problems. Utility Model Content
[0005] The disclosed embodiments provide an intelligent cell culture control system to solve the problem that the method for controlling parameters of a cell culture tank is relatively single.
[0006] The present disclosure provides an intelligent cell culture control system, comprising:
[0007] Multiple monitoring modules, a logic judgment module, a first control module, a second control module, and multiple operation modules;
[0008] The multiple monitoring modules are all arranged in a cell culture tank;
[0009] The multiple monitoring modules are respectively connected to the logic judgment module; the logic judgment module is respectively connected to the first control module and the second control module;
[0010] The first control module is connected to the plurality of operating modules respectively; the first control module is configured to control each operating module individually;
[0011] The second control module is connected to the multiple operation modules respectively; the second control module is configured to control the multiple operation modules simultaneously;
[0012] The multiple operation modules are all arranged inside the cell culture tank.
[0013] In an exemplary embodiment of the present disclosure, the plurality of monitoring modules include: a first monitoring module, a second monitoring module, and a third monitoring module;
[0014] The logic judgment module includes:
[0015] Comparator U1, comparator U2, comparator U3;
[0016] A first end of the comparator U1 is connected to the first monitoring module, a second end of the comparator U1 is used to connect to the first input source, and a third end of the comparator U1 is connected to the first control module and the second control module respectively;
[0017] A first end of the comparator U2 is connected to the second monitoring module, a second end of the comparator U2 is used to connect to a second input source, and a third end of the comparator U2 is connected to the first control module and the second control module respectively;
[0018] A first end of the comparator U3 is connected to the third monitoring module, a second end of the comparator U3 is used to connect to a third input source, and a third end of the comparator U3 is connected to the first control module and the second control module respectively.
[0019] In an exemplary embodiment of the present disclosure, the multiple operation modules include:
[0020] Heating pipes, gas pipelines and stirring motors;
[0021] The heating pipe is used to heat liquid; the gas pipeline is used to transport gas; the heating pipe and the gas pipeline are arranged in sequence at the bottom of the cell culture tank; the stirring motor is arranged at the top of the cell culture tank.
[0022] In an exemplary embodiment of the present disclosure, the first control module includes:
[0023] Resistor R1, resistor R2, resistor R3, transistor Q1, transistor Q2, transistor Q3, diode D1, diode D2, diode D3, relay J1, relay J2, relay J3;
[0024] The first end of the resistor R1, the first end of the resistor R2, and the first end of the resistor R3 are all connected to the logic judgment module;
[0025] The base of the transistor Q1 is connected to the second end of the resistor R2, the collector of the transistor Q1 is connected to the anode of the diode D1 and the first end of the relay J1 respectively, the emitter of the transistor Q1 is grounded, the cathode of the diode D1 and the second end of the relay J1 are both connected to VCC; the normally open contact of the relay J1 is used to control the switch of the heating pipe;
[0026] The base of the transistor Q2 is connected to the second end of the resistor R2, the collector of the transistor Q2 is connected to the anode of the diode D2 and the first end of the relay J2 respectively, the emitter of the transistor Q2 is grounded, the cathode of the diode D2 and the second end of the relay J2 are both connected to VCC; the normally open contact of the relay J2 is used to control the on and off of the gas pipeline;
[0027] The base of the transistor Q3 is connected to the second end of the resistor R3, the collector of the transistor Q3 is connected to the positive electrode of the diode D3 and the first end of the relay J3 respectively, the emitter of the transistor Q3 is grounded, the negative electrode of the diode D3 and the second end of the relay J3 are both connected to VCC; the normally open contact of the relay J3 is used to control the start and stop of the stirring motor.
[0028] In an exemplary embodiment of the present disclosure, the second control module includes:
[0029] AND gate U4, transistor Q4, relay J4, diode D4, resistor R4;
[0030] The first end, the second end and the third end of the AND gate U4 are all connected to the logic judgment module, the fourth end of the AND gate U4 is connected to the first end of the resistor R4, and the second end of the resistor R4 is connected to the base of the transistor Q4;
[0031] The collector of the transistor Q4 is connected to the anode of the diode D4 and the first end of the relay J4 respectively, the emitter of the transistor Q4 is grounded, and the cathode of the diode D4 and the second end of the relay J4 are both connected to VCC.
[0032] In an exemplary embodiment of the present disclosure, the relay J4 includes:
[0033] a first normally open contact, a second normally open contact, and a third normally open contact;
[0034] The first normally open contact is connected in parallel with the normally open contact of the relay J1;
[0035] The second normally open contact is connected in parallel with the normally open contact of relay J2;
[0036] The third normally open contact is connected in parallel with the normally open contact of the relay J3.
[0037] In an exemplary embodiment of the present disclosure, a cell culture intelligent control system further includes:
[0038] Multiple alarm modules;
[0039] The multiple monitoring modules are respectively connected to the multiple alarm modules in a one-to-one correspondence.
[0040] In an exemplary embodiment of the present disclosure, a cell culture intelligent control system further includes:
[0041] Display module;
[0042] The multiple monitoring modules are all connected to the display module.
[0043] The beneficial effects of the cell culture intelligent control system provided by the embodiments of the present disclosure are:
[0044] First, the embodiment of the present disclosure can select the first control module or the second control module according to the monitoring results of multiple monitoring modules to realize automatic regulation of the internal environment without human intervention, thereby realizing intelligent control of the cell culture tank, improving the control effect during the cell culture process, and increasing the probability of successful cell culture.
[0045] Second, the embodiment of the present disclosure can select different control methods to regulate different environmental indicators in the cell culture tank, realize individual control of each environmental indicator according to the first control module, realize unified control of all environmental indicators through the second control module, and combine multiple control methods to realize precise control of the cell culture tank, thereby improving control flexibility and accuracy. BRIEF DESCRIPTION OF THE DRAWINGS
[0046] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.
[0047] Figure 1 This is a schematic structural diagram of a cell culture intelligent control system provided by an embodiment of the present disclosure;
[0048] Figure 2 This is a circuit diagram of a cell culture intelligent control system provided by one embodiment of the present disclosure;
[0049] Figure 3 This is a circuit diagram of a cell culture intelligent control system provided by another embodiment of the present disclosure. DETAILED DESCRIPTION
[0050] To help those skilled in the art better understand this solution, the following will clearly describe the technical solutions in the embodiments of this solution in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of this solution, not all of it. Based on the embodiments of this solution, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of this solution.
[0051] Throughout the specification, claims, and accompanying figures of this solution, the term "including" and any variations thereof mean "including, but not limited to," and are intended to cover non-exclusive inclusions and are not limited to the examples listed herein. Furthermore, the terms "first" and "second," etc., are used to distinguish between different objects, not to describe a specific order.
[0052] The following describes the implementation of the present disclosure in detail with reference to the accompanying drawings:
[0053] Figure 1 This is a schematic diagram of the structure of a cell culture intelligent control system provided by the embodiment of the present disclosure. Figure 1 , the cell culture intelligent control system 10 includes:
[0054] Multiple monitoring modules 11 , a logic judgment module 12 , a first control module 13 , a second control module 14 , and multiple operation modules 15 .
[0055] The multiple monitoring modules 11 are all arranged in the cell culture tank.
[0056] The plurality of monitoring modules 11 are respectively connected to the logic judgment module 12. The logic judgment module 12 is respectively connected to the first control module 13 and the second control module 14.
[0057] The first control module 13 is respectively connected to the plurality of operating modules 15. The first control module 13 is configured to control each operating module individually.
[0058] The second control module 14 is respectively connected to the plurality of operating modules 15. The second control module 14 is configured to control the plurality of operating modules 15 simultaneously.
[0059] The multiple operation modules 15 are all arranged inside the cell culture tank.
[0060] In this embodiment, the multiple monitoring modules 11 may include a variety of monitoring sensors for monitoring various environmental parameters within the cell culture tank. For example, the multiple monitoring modules 11 may include temperature sensors, humidity sensors, rotation speed sensors, pH monitors, concentration sensors, etc., respectively used to monitor parameters such as temperature, humidity, stirring motor speed, pH value, oxygen concentration, and carbon dioxide concentration within the cell culture tank. These parameters are important indicators of cell growth and reproduction and are crucial to the success of cell culture. The multiple monitoring modules 11 transmit various monitoring data to the logic judgment module 12, which compares the monitoring data from the multiple monitoring modules 11 with standard data.
[0061] The logic judgment module 12 serves as the central decision-making unit of the entire control system. It receives real-time data from multiple monitoring modules 11 to determine whether the current culture environment meets optimal culture conditions or whether any environmental indicators need adjustment. Based on the comparison results, it selects whether to use the first control module 13 to adjust a single environmental indicator, or to use the second control module 14 to comprehensively adjust all environmental indicators, ensuring that the cell culture tank always maintains a suitable environment for cell growth.
[0062] The first control module 13 and the second control module 14 respectively assume different control strategies, which enhances the flexibility and response speed of the system. The first control module 13 is responsible for individually controlling each operating module to achieve fine-grained adjustment. For example, if it is monitored that the temperature in the current cell culture tank is too low and the heater in the culture tank needs to be adjusted separately, the heater can be started separately by the first control module 13 to raise the temperature in the cell culture tank to a suitable temperature without causing changes in other environmental parameters. For example, if it is monitored that the speed of the stirring motor in the current cell culture tank is low, so that the liquid in the cell culture tank cannot be evenly mixed, the speed of the stirring motor can be adjusted by the first control module 13.
[0063] The second control module 14 is capable of simultaneously controlling multiple operating modules 15 and is suitable for rapid overall environmental adjustments. For example, if an emergency requires rapid restoration of the entire cell culture tank environment to a standard state, the second control module 14 can be used to uniformly adjust all environmental parameters.
[0064] Multiple operating modules 15: These modules can directly operate within the cell culture tank, executing instructions issued by the first control module 13 and the second control module 14. For example, the multiple operating modules 15 can include: adjusting temperature, humidity, pH value, gas concentration, stirring speed, etc. to ensure a stable and optimized environment within the cell culture tank.
[0065] From the above, it can be concluded that the cell culture intelligent control system 10 realizes comprehensive automated regulation of the environment inside the cell culture tank through multi-level monitoring, intelligent judgment and flexible control, which can not only effectively improve the quality and efficiency of cell culture, but also reduce human operation errors and improve the accuracy and automation level of cell culture tank control.
[0066] Figure 2 This is a circuit diagram of a cell culture intelligent control system provided by an embodiment of the present disclosure, referring to Figure 2 In one embodiment of the present disclosure, the plurality of monitoring modules 11 include: a first monitoring module, a second monitoring module, and a third monitoring module.
[0067] The logic judgment module 12 includes:
[0068] Comparator U1, comparator U2, comparator U3.
[0069] A first end of the comparator U1 is connected to the first monitoring module, a second end of the comparator U1 is used to connect to the first input source, and a third end of the comparator U1 is connected to the first control module 13 and the second control module 14 respectively.
[0070] A first end of the comparator U2 is connected to the second monitoring module, a second end of the comparator U2 is used to connect to the second input source, and a third end of the comparator U2 is connected to the first control module 13 and the second control module 14 respectively.
[0071] A first end of the comparator U3 is connected to the third monitoring module, a second end of the comparator U3 is used to connect to the third input source, and a third end of the comparator U3 is connected to the first control module 13 and the second control module 14 respectively.
[0072] In this embodiment, the multiple monitoring modules 11 can be further divided into a first monitoring module, a second monitoring module and a third monitoring module, which correspond to different monitoring parameters, such as temperature, carbon dioxide concentration, stirring speed, etc., to ensure comprehensive coverage of key environmental variables in the cell culture tank.
[0073] The logic judgment module 12 can implement real-time comparison of monitoring data by integrating comparators U1, U2, and U3. The first terminal of comparator U1 is connected to the first monitoring module, and the second terminal is connected to a first input source. The first input source is an ideal parameter value or a preset parameter value that is the same as the environmental indicator corresponding to the first monitoring module. The third terminal of comparator U1 sends an adjustment signal to the first control module 13 and the second control module 14 based on the difference between the monitored value of the first environmental indicator and the standard value.
[0074] Comparator U2 and comparator U3 follow similar principles and correspond to the real-time monitoring and deviation judgment of the other two key environmental indicators respectively. Comparator U2 is connected to the second monitoring module and the second input source respectively, wherein the second input source is the ideal parameter value or preset parameter value that is the same as the environmental indicator corresponding to the second monitoring module. The third end of comparator U2 sends an adjustment signal to the first control module 13 and the second control module 14 based on the difference between the monitored value and the standard value of the second environmental indicator. Comparator U3 is connected to the third monitoring module and the third input source respectively, wherein the third input source is the ideal parameter value or preset parameter value that is the same as the environmental indicator corresponding to the third monitoring module. The third end of comparator U3 sends an adjustment signal to the first control module 13 and the second control module 14 based on the difference between the monitored value and the standard value of the third environmental indicator.
[0075] The first control module 13 and the second control module 14 respectively perform precise single adjustments (first control module 13) or synchronous comprehensive adjustments (second control module 14) based on the output of the comparator, ensuring rapid response and fine regulation of the culture environment. They can handle both fine-tuning of individual parameters and meet the overall optimization needs of the environment.
[0076] From the above, it can be concluded that this embodiment achieves real-time monitoring, intelligent judgment, and precise control of the cell culture process through highly integrated hardware logic. This not only improves the efficiency and success rate of cell culture, but also optimizes the level of automation of operations and reduces the process of manual intervention. It is an important advancement in cell culture automation technology.
[0077] In one embodiment of the present disclosure, the plurality of operation modules 15 include:
[0078] Heating pipes, gas pipelines and stirring motors.
[0079] The heating pipe is used to heat liquid. The gas pipe is used to transport gas. The heating pipe and gas pipe are arranged sequentially at the bottom of the cell culture tank. The stirring motor is installed at the top of the cell culture tank.
[0080] In this embodiment, temperature control can be achieved through a heating pipe, which can be located at the bottom of the cell culture tank. By precisely controlling the heating power, the temperature of the culture medium can be quickly and evenly adjusted to meet the specific temperature requirements of different cell types and maintain an optimal growth environment.
[0081] For example, the heating pipe can be manually set to a heating temperature through PID (Proportional Integral Derivative) control. When the heating pipe or heater is started, it can be automatically heated to the set temperature to ensure a constant temperature inside the cell culture tank.
[0082] The regulation of carbon dioxide concentration, oxygen concentration or pH value can be achieved through the gas pipeline, which is also set at the bottom of the cell culture tank. Carbon dioxide or oxygen is input into the cell culture tank through the gas pipeline to change the internal gas concentration or pH value, ensuring a stable supply of gas to maintain the appropriate ratio of gas components in the culture medium, which is crucial for cell respiration and metabolism.
[0083] For example, gas concentration can be controlled by manually setting the gas pump value in advance. When the gas pipeline is activated, the corresponding gas is fed into the gas pipeline according to the set value of the gas pump. PH value can be adjusted by manually setting the value of the acid pump or alkali pump. When the gas pipeline is activated, carbon dioxide gas or alkaline gas is automatically fed into the gas pipeline through the acid pump or alkali pump until the pH value inside the cell culture tank reaches the preset value, thereby ensuring the stability of the pH value inside the cell culture tank.
[0084] The stirring speed can be controlled by the stirring motor. By adjusting the speed of the internal motor, the liquid in the cell culture tank can be quickly and evenly stirred. The stirring motor can be installed on the top of the cell culture tank. By rotating the stirring blade, the stirring motor promotes the uniform mixing of substances in the culture medium, improves the uniformity of nutrient distribution, and increases the amount of dissolved oxygen, ensuring the uniformity and efficiency of cell culture.
[0085] For example, the stirring motor can be equipped with a variable speed pump or a fixed speed pump. The speed range of the variable speed pump can be controlled within 0-180 rpm. The speed of the fixed speed pump can be set to 18 rpm.
[0086] In addition, the injection of liquid into the cell culture tank can be achieved through a peristaltic pump, and the speed of liquid injection can be adjusted by adjusting the flow rate of the peristaltic pump. The peristaltic pump can adopt volume mode: set the speed and duty cycle in advance, and when the peristaltic pump is started, the flow accumulation begins. When the accumulated amount reaches the set volume value, the peristaltic pump can be controlled to stop running. When the peristaltic pump adopts volume mode, the flow rate of the peristaltic pump can be calibrated in advance. The peristaltic pump can also adopt time mode: operate according to the set speed and duty cycle, and the timing starts when the peristaltic pump is turned on. When the timing reaches the set value of the time control, the peristaltic pump stops running. According to different needs, you can choose the corresponding mode to regulate the environment in the cell culture tank.
[0087] As can be seen from the above, this embodiment not only considers the functionality of the operating modules, but also fully considers space utilization and operational efficiency. Through the precise regulation of the intelligent control module, the entire system achieves comprehensive automated management of the cell culture environment, effectively promoting cell growth and differentiation, and improving scientific research and production efficiency.
[0088] Reference Figure 2 In one embodiment of the present disclosure, the first control module 13 includes:
[0089] Resistor R1, resistor R2, resistor R3, transistor Q1, transistor Q2, transistor Q3, diode D1, diode D2, diode D3, relay J1, relay J2, relay J3.
[0090] The first end of the resistor R1 , the first end of the resistor R2 , and the first end of the resistor R3 are all connected to the logic judgment module 12 .
[0091] The base of transistor Q1 is connected to the second end of resistor R2. The collector of transistor Q1 is connected to the anode of diode D1 and the first end of relay J1, respectively. The emitter of transistor Q1 is grounded, and the cathode of diode D1 and the second end of relay J1 are both connected to VCC. The normally open contact of relay J1 is used to control the heating pipe on and off.
[0092] The base of transistor Q2 is connected to the second end of resistor R2. The collector of transistor Q2 is connected to the anode of diode D2 and the first end of relay J2, respectively. The emitter of transistor Q2 is grounded, and the cathode of diode D2 and the second end of relay J2 are both connected to VCC. The normally open contact of relay J2 is used to control the on / off of the gas pipeline.
[0093] The base of transistor Q3 is connected to the second end of resistor R3. The collector of transistor Q3 is connected to the anode of diode D3 and the first end of relay J3, respectively. The emitter of transistor Q3 is grounded, and the cathode of diode D3 and the second end of relay J3 are both connected to VCC. The normally open contact of relay J3 is used to control the start and stop of the stirring motor.
[0094] In this embodiment, the three resistors R1, R2, and R3 serve as pull-up or voltage-dividing resistors and are connected to the logic judgment module 12 to limit the current flowing into the base of the transistor and convert the control signal output by the logic judgment module 12 into a voltage level suitable for the base of the transistor.
[0095] Transistors Q1, Q2, and Q3 act as switching elements. Based on the signal from the logic judgment module 12, they control the current flowing through the relay coil, thereby controlling the start and stop of the heating pipe, gas pipeline, and stirring motor. The base of transistor Q1 is connected to the logic judgment module 12 via resistor R1, the collector is connected to the first end of relay J1 via diode D1, and the emitter is grounded, forming a current loop. Diode D1 provides protection, preventing the back electromotive force generated when the relay J1 coil is powered off from damaging transistor Q1. Relay J1 controls the heating pipe. When the corresponding transistor Q1 is turned on, the relay J1 coil is energized, and its normally open contacts are closed, thereby controlling the working state of the heating pipe, which is used to heat liquids.
[0096] The base of transistor Q2 is connected to logic judgment module 12 via resistor R2. Its collector is connected to the first terminal of relay J2 via diode D2, and its emitter is grounded, forming a current loop. Diode D2 provides protection, preventing damage to transistor Q2 from the back electromotive force generated when relay J2's coil is de-energized. Relay J2 controls the gas pipeline. When the corresponding transistor Q2 is turned on, relay J2's coil is energized, closing its normally open contacts and controlling the operating state of the gas pipeline, which is used to transport various gases.
[0097] The base of transistor Q3 is connected to logic judgment module 12 via resistor R3. Its collector is connected to the first terminal of relay J3 via diode D3, and its emitter is grounded, forming a current loop. Diode D3 provides protection, preventing damage to transistor Q3 from the back electromotive force generated when relay J3's coil is de-energized. Relay J3 controls the stirring motor. When the corresponding transistor Q3 is turned on, relay J3's coil is energized, closing its normally open contacts, thereby controlling the operating state of the stirring motor, which is used to stir the culture medium.
[0098] VCC represents the positive power supply of the circuit, which provides the required voltage for the entire control circuit.
[0099] As can be seen from the above, in this embodiment, the first control module 13 can respond to the instructions of the logic judgment module 12 and independently control each operating module to achieve precise adjustment of the cell culture environment. This design embodies the application of electronic circuits in the field of automated control and ensures the stability and efficiency of the cell culture process.
[0100] Reference Figure 2 In one embodiment of the present disclosure, the second control module 14 includes:
[0101] AND gate U4, transistor Q4, relay J4, diode D4, resistor R4.
[0102] The first, second and third terminals of the AND gate U4 are connected to the logic judgment module 12 , the fourth terminal of the AND gate U4 is connected to the first terminal of the resistor R4 , and the second terminal of the resistor R4 is connected to the base of the transistor Q4 .
[0103] The collector of the transistor Q4 is connected to the anode of the diode D4 and the first end of the relay J4 respectively, the emitter of the transistor Q4 is grounded, and the cathode of the diode D4 and the second end of the relay J4 are both connected to VCC.
[0104] In this embodiment, AND gate U4 can be an AND gate, whose three input terminals are respectively connected to logic judgment module 12. The integrated signal output by logic judgment module 12 determines whether to open or close relay J4. The output (fourth terminal) of AND gate U4 is connected to resistor R4, and together they control the state of transistor Q4, demonstrating the module's logic judgment and signal integration capabilities.
[0105] Transistor Q4 serves as the driver for relay J4. Its base is connected to the output of AND gate U4 via resistor R4. Its collector is connected to the anode of diode D4 and the first terminal of relay J4, and its emitter is grounded. Transistor Q4 is turned on or off based on the control signal from AND gate U4, thereby controlling the operation of relay J4.
[0106] In this embodiment, relay J4 may be designed to simultaneously control multiple operating modules 15 (such as simultaneously turning on or off the heating pipe, gas pipeline, and stirring motor). The closing and opening of its normally open contact are indirectly controlled by transistor Q4, thereby achieving rapid overall adjustment of the cell culture environment.
[0107] Diode D4 protects transistor Q4 from the back electromotive force generated when the coil of relay J4 is de-energized. Resistor R4 provides an appropriate bias resistor for the base of transistor Q4, ensuring that the transistor can correctly respond to the output signal of AND gate U4.
[0108] From the above, it can be concluded that after receiving the comprehensive control signal from the logic judgment module 12, the second control module 14 of this embodiment can quickly perform simultaneous control of multiple operation modules 15, which is suitable for scenarios that require rapid response to overall environmental changes, thereby improving the automation level and response speed of the cell culture process.
[0109] Figure 3 This is a circuit diagram of a cell culture intelligent control system provided by another embodiment of the present disclosure, referring to Figure 3 In one embodiment of the present disclosure, the relay J4 includes:
[0110] A first normally open contact, a second normally open contact, and a third normally open contact.
[0111] The first normally open contact is connected in parallel with the normally open contact of the relay J1.
[0112] The second normally open contact is connected in parallel with the normally open contact of relay J2.
[0113] The third normally open contact is connected in parallel with the normally open contact of relay J3.
[0114] In this embodiment, the design of relay J4 further enhances the flexibility and response speed of the system, and realizes the simultaneous control process of multiple operation modules 15 through the parallel connection of its three normally open contacts with a single operation control relay (J1, J2, J3).
[0115] Relay J4 may include a first normally open contact, a second normally open contact and a third normally open contact. This configuration allows J4 to act as a "master control" relay, capable of simultaneously affecting multiple operating modules 15 under a single instruction, namely the control of the heating pipeline, the gas pipeline and the stirring motor.
[0116] The first normally open contact is connected in parallel with the normally open contact of relay J1. When relay J4 is activated, its first normally open contact closes and is connected in parallel with the normally open contact of relay J1, jointly controlling the opening of the heating pipe to achieve a fast heating response.
[0117] Similarly, the second normally open contact of relay J4 is connected in parallel with the normally open contact of relay J2, jointly controlling the on / off of the gas pipeline and ensuring immediate adjustment of the gas supply. The third normally open contact of relay J4 is connected in parallel with the normally open contact of the stirring motor control relay J3, synchronously controlling the start or stop of the stirring motor to maintain the uniformity of the culture medium.
[0118] As can be seen from the above, this embodiment simplifies the operation process of adjusting multiple parameters at the same time, so that when a quick response or execution of a specific program is required (such as environmental recovery in an emergency situation, switching of a preset culture program), it is only necessary to send a single instruction to the second control module 14 through the logic judgment module 12, and the synchronous control of heating, gas supply and stirring can be triggered at one time through the relay J4, thereby greatly improving the degree of automation and emergency handling capabilities of the cell culture process.
[0119] In one embodiment of the present disclosure, a cell culture intelligent control system 10 further includes:
[0120] Multiple alarm modules.
[0121] The plurality of monitoring modules 11 are connected to the plurality of alarm modules in a one-to-one correspondence.
[0122] In this embodiment, multiple alarm modules are configured to draw the operator's attention promptly to any anomalies in each environmental parameter. Each alarm module is linked to its corresponding monitoring module. This design ensures immediate alerts for any deviations from predefined parameters during the cell culture process. Each monitoring module monitors a key parameter (such as temperature, pH, or gas concentration). If the monitored data exceeds a predefined safety range or reaches an alert level, the corresponding alarm module is activated.
[0123] This one-to-one connection ensures targeted and accurate alarms. For example, if the first monitoring module detects an abnormal rise in culture fluid temperature, it immediately notifies its corresponding alarm module, which then activates, emitting audible, visual, or other alarm signals to alert the operator or automatically initiate emergency measures, such as calling the control module to adjust parameters or perform an emergency shutdown, to prevent deterioration of the cell culture environment and protect the safety of cell samples and equipment.
[0124] It can be concluded from the above that this embodiment not only enhances the self-monitoring and fault response capabilities of the control system, but also improves the reliability of the entire cell culture process and the intervention efficiency of the operator, ensuring the continuity and success rate of cell culture experiments or production.
[0125] In one embodiment of the present disclosure, a cell culture intelligent control system 10 further includes:
[0126] Display module.
[0127] The plurality of monitoring modules 11 are all connected to the display module.
[0128] In this embodiment, to facilitate the operator's timely observation of various environmental parameters in the current cell culture tank, a display module can be provided to receive and display real-time data from multiple monitoring modules 11. This means that all key monitoring parameters, including but not limited to temperature, pH value, oxygen concentration, carbon dioxide concentration, humidity, stirring motor speed, etc., can be intuitively presented to the operator or researcher on the display module, allowing them to understand the status of the cell culture environment at any time.
[0129] By connecting directly to each monitoring module, the display module can update and integrate all monitoring data in real time, providing a unified and easy-to-interpret view. This design not only simplifies the data acquisition process and improves the timeliness of data, but also makes the monitoring process more efficient. Operators can gain a comprehensive understanding of the entire culture environment without having to check each monitoring point one by one.
[0130] From the above, it can be concluded that this embodiment can greatly improve the transparency and convenience of experimental operations through the display module, allowing operators to quickly respond to environmental changes and make corresponding adjustments, which helps promote the standardization and efficiency of cell culture research and production processes.
[0131] The above embodiments are only used to illustrate the technical solutions of the present disclosure, rather than to limit them. Although the present disclosure has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present disclosure.
Claims
1. A cell culture intelligent control system, characterized in that: include: Multiple monitoring modules, a logic judgment module, a first control module, a second control module, and multiple operation modules; The multiple monitoring modules are all arranged in a cell culture tank; The multiple monitoring modules are respectively connected to the logic judgment module; the logic judgment module is respectively connected to the first control module and the second control module; The first control module is connected to the plurality of operating modules respectively; the first control module is configured to control each operating module individually; The second control module is connected to the multiple operation modules respectively; the second control module is configured to control the multiple operation modules simultaneously; The multiple operation modules are all arranged inside the cell culture tank; The multiple monitoring modules include: a first monitoring module, a second monitoring module, and a third monitoring module; The logic judgment module includes: Comparator U1, comparator U2, comparator U3; A first end of the comparator U1 is connected to the first monitoring module, a second end of the comparator U1 is used to connect to the first input source, and a third end of the comparator U1 is connected to the first control module and the second control module respectively; A first end of the comparator U2 is connected to the second monitoring module, a second end of the comparator U2 is used to connect to a second input source, and a third end of the comparator U2 is connected to the first control module and the second control module respectively; A first end of the comparator U3 is connected to the third monitoring module, a second end of the comparator U3 is used to connect to a third input source, and a third end of the comparator U3 is connected to the first control module and the second control module respectively.
2. The cell culture intelligent control system according to claim 1, characterized in that: The multiple operation modules include: Heating pipes, gas pipelines and stirring motors; The heating pipe is used to heat liquid; the gas pipeline is used to transport gas; the heating pipe and the gas pipeline are arranged in sequence at the bottom of the cell culture tank; the stirring motor is arranged at the top of the cell culture tank.
3. The cell culture intelligent control system according to claim 2, characterized in that: The first control module includes: Resistor R1, resistor R2, resistor R3, transistor Q1, transistor Q2, transistor Q3, diode D1, diode D2, diode D3, relay J1, relay J2, relay J3; The first end of the resistor R1, the first end of the resistor R2, and the first end of the resistor R3 are all connected to the logic judgment module; The base of the transistor Q1 is connected to the second end of the resistor R2, the collector of the transistor Q1 is connected to the anode of the diode D1 and the first end of the relay J1 respectively, the emitter of the transistor Q1 is grounded, the cathode of the diode D1 and the second end of the relay J1 are both connected to VCC; the normally open contact of the relay J1 is used to control the switch of the heating pipe; The base of the transistor Q2 is connected to the second end of the resistor R2, the collector of the transistor Q2 is connected to the anode of the diode D2 and the first end of the relay J2 respectively, the emitter of the transistor Q2 is grounded, the cathode of the diode D2 and the second end of the relay J2 are both connected to VCC; the normally open contact of the relay J2 is used to control the on and off of the gas pipeline; The base of the transistor Q3 is connected to the second end of the resistor R3, the collector of the transistor Q3 is connected to the positive electrode of the diode D3 and the first end of the relay J3 respectively, the emitter of the transistor Q3 is grounded, the negative electrode of the diode D3 and the second end of the relay J3 are both connected to VCC; the normally open contact of the relay J3 is used to control the start and stop of the stirring motor.
4. The cell culture intelligent control system according to claim 2, characterized in that: The second control module includes: AND gate U4, transistor Q4, relay J4, diode D4, resistor R4; The first end, the second end and the third end of the AND gate U4 are all connected to the logic judgment module, the fourth end of the AND gate U4 is connected to the first end of the resistor R4, and the second end of the resistor R4 is connected to the base of the transistor Q4; The collector of the transistor Q4 is connected to the anode of the diode D4 and the first end of the relay J4 respectively, the emitter of the transistor Q4 is grounded, and the cathode of the diode D4 and the second end of the relay J4 are both connected to VCC.
5. The cell culture intelligent control system according to claim 4, characterized in that: The relay J4 includes: a first normally open contact, a second normally open contact, and a third normally open contact; The first normally open contact is connected in parallel with the normally open contact of the relay J1; The second normally open contact is connected in parallel with the normally open contact of relay J2; The third normally open contact is connected in parallel with the normally open contact of the relay J3.
6. The cell culture intelligent control system according to claim 1, characterized in that: Also includes: Multiple alarm modules; The multiple monitoring modules are respectively connected to the multiple alarm modules in a one-to-one correspondence.
7. The cell culture intelligent control system according to claim 1, characterized in that: Also includes: Display module; The multiple monitoring modules are all connected to the display module.