Cell cryopreservation drawer

Abstract

The utility model discloses a cell cryopreservation drawer which comprises a main body mechanism, the main body mechanism comprises a base, a cabinet body fixed to the top end of the base, a plurality of sealing doors installed on the cabinet body, a box body installed on the inner wall of the cabinet body, a main liquid outlet pipe arranged on one side of the cabinet body, a plurality of first connecting pipes installed on one side of the main liquid outlet pipe and a pump machine installed on the inner wall of the base. The bottom end of the main liquid outlet pipe communicates with the box body. The base is used for installing other assemblies, the cabinet body is made of a thermal insulation material and is used for cryopreserving cells, a plurality of partition plates are fixed on the inner wall of the cabinet body in an array mode, an inner cavity of the cabinet body is divided into a plurality of storage cavities, and the storage cavities are used for storing the cells. The cell freezing and storing drawer has the advantages that the cells can be independently stored, and the waste of liquid nitrogen is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell freezing, in particular to a cell freezing drawer. Background Art

[0002] Cells are the basic structural and functional units of living organisms. All organisms, with the exception of viruses, are composed of cells, though viruses must function within them. Cells are extremely small, visible only under a microscope, and come in a variety of shapes. They are primarily composed of a nucleus and cytoplasm, with a cell membrane on their surface. Higher plant cells have a cell wall outside the cell membrane, often containing plastids within the cytoplasm, chloroplasts, vacuoles, and mitochondria within the cell. Animal cells lack cell walls and often have centrioles within their cytoplasm, which are absent in higher plant cells. Cells perform functions such as movement, nutrition, and reproduction. During various experiments, cryostat storage is required to maintain cell activity, typically by submerging the cells in liquid nitrogen.

[0003] Existing cell freezing drawers have the following main drawbacks during use: when the freezing cabinet is opened to access cells, a large amount of liquid nitrogen in the cabinet will be vaporized, thereby increasing the temperature of the cabinet cavity and affecting other stored cells. Therefore, there is room for improvement. Utility Model Content

[0004] The utility model aims to solve one of the technical problems existing in the prior art or related technologies.

[0005] To this end, the technical solution adopted by the present invention is: a cell freezing drawer, comprising: a main body mechanism, the main body mechanism including a base, a cabinet fixed on the top of the base, a plurality of sealed doors installed on the cabinet, a box installed on the inner wall of the cabinet, a main liquid outlet pipe arranged on one side of the cabinet and connected to the box at the bottom end, a plurality of first connecting pipes installed on one side of the main liquid outlet pipe, a pump installed on the inner wall of the base, a main liquid inlet pipe arranged on the other side of the cabinet and connected to the pump outlet, and a plurality of second connecting pipes installed on the main liquid inlet pipe.

[0006] A plurality of partitions are fixed in an array on the inner wall of the cabinet to divide the inner cavity of the cabinet into a plurality of storage cavities. Each storage cavity corresponds to a sealed door. A liquid outlet channel is provided at the bottom end of the storage cavity to communicate with the first connecting pipe, and a liquid inlet channel is provided at the top end of the storage cavity to communicate with the second connecting pipe.

[0007] A temperature sensor is installed on the inner top wall of the storage cavity, and the second connecting pipe includes a second branch pipe connecting the liquid inlet channel and the main liquid inlet pipe and a flow valve installed on the second branch pipe.

[0008] In a preferred example, the present invention can be further configured as follows: the sealed door includes a door panel hingedly mounted on the cabinet body, a control panel mounted on the door panel, and a handle fixed on the door panel.

[0009] In a preferred example, the present invention can be further configured as follows: the first connecting pipe includes a first branch pipe connecting the liquid outlet channel and the main liquid outlet pipe, and an electric valve installed on the first branch pipe.

[0010] In a preferred example, the present invention can be further configured as follows: the inlet of the pump is connected to the inner cavity of the box through a water pumping pipe, and the outlet of the pump is connected to the end of the main liquid inlet pipe.

[0011] In a preferred example, the present invention can be further configured as follows: the cabinet, the main liquid inlet pipe, the box body and the main liquid outlet pipe are all made of thermal insulation materials.

[0012] By adopting the above technical solution, the beneficial effects achieved by the utility model are as follows:

[0013] 1. In the present invention, a plurality of partitions are fixed on the inner wall of the cabinet to divide the inner cavity of the cabinet into a plurality of independent storage cavities, and each storage space is individually provided with a sealed door. Through the above arrangement, it is possible to avoid the cabinet being opened when taking cells, resulting in a large amount of liquid nitrogen being vaporized and affecting the storage environment of other cells. At the same time, a box is arranged in the inner cavity of the base, and a main liquid outlet pipe is arranged on the base to communicate with the water tank, and a liquid outlet channel is arranged at the bottom end of each storage cavity. The liquid outlet channel corresponding to each storage cavity is connected to the main liquid outlet pipe through a first connecting pipe. A main liquid inlet pipe is arranged on one side of the cabinet, and a pump is installed in the base. One end of the pump is connected to the box, and the other end is connected to the main liquid inlet pipe. At the same time, a liquid inlet channel is provided at the top of each storage cavity, and the liquid inlet channel corresponding to each storage cavity is connected to the main liquid inlet channel through the second connecting pipe. When taking cells, the first connecting pipe corresponding to the storage cavity where the cells are placed is opened, so that the liquid nitrogen in the storage cavity passes through the liquid outlet channel, the first connecting pipe, and the main liquid outlet pipe and enters the box for temporary storage. After the taking is completed, the sealing door is closed. At this time, the pump re-pumps the liquid nitrogen temporarily stored in the box through the main liquid inlet pipe, the second connecting pipe and the liquid inlet channel into the storage cavity. Through the above arrangement, the waste of liquid nitrogen caused by the large amount of liquid nitrogen vaporizing when sampling cells can be avoided, and the practical performance is further improved.

[0014] 2. In the present invention, a temperature sensor is installed on the inner top wall of each storage cavity, and the second connecting pipe is composed of a second branch pipe and a flow valve. When liquid nitrogen is re-injected into the storage cavity, the flow valve controls the liquid nitrogen to be slowly injected into the storage cavity, thereby ensuring that the temperature in the storage cavity is slowly lowered, avoiding the formation of ice crystals in the cells during the freezing process, which may cause damage to the cells, and further increasing practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural diagram of the utility model;

[0016] Figure 2 It is a cross-sectional schematic diagram of the utility model;

[0017] Figure 3 This is a schematic diagram of the cross-sectional structure of the partition of the present utility model.

[0018] Reference numerals:

[0019] 100. Main body; 110. Base; 120. Cabinet; 121. Partition; 122. Storage cavity; 123. Liquid outlet channel; 124. Liquid inlet channel; 125. Temperature sensor; 130. Sealing door; 131. Door panel; 132. Control panel; 133. Handle; 140. Box; 150. Main liquid outlet pipe; 160. First connecting pipe; 161. First branch pipe; 162. Electric valve; 170. Pump; 171. Suction pipe; 180. Main liquid inlet pipe; 190. Second connecting pipe; 191. Second branch pipe; 192. Flow valve. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. It should be noted that the embodiments of the present invention and the features therein can be combined with each other unless there is any conflict.

[0021] Some embodiments of the present invention are described below with reference to the accompanying drawings.

[0022] Example 1:

[0023] Combine Figure 1-3 As shown, this embodiment provides a cell freezing drawer, including: a main body mechanism 100.

[0024] The main body mechanism 100 includes a base 110, a cabinet 120 fixed to the top of the base 110, a plurality of sealed doors 130 installed on the cabinet 120, a box 140 installed on the inner wall of the cabinet 120, a main liquid outlet pipe 150 arranged on one side of the cabinet 120 and connected to the box 140 at the bottom end, a plurality of first connecting pipes 160 installed on one side of the main liquid outlet pipe 150, a pump 170 installed on the inner wall of the base 110, a main liquid inlet pipe 180 arranged on the other side of the cabinet 120 and connected to the outlet of the pump 170, and a plurality of second connecting pipes 190 installed on the main liquid inlet pipe 180.

[0025] The base 110 is used to install other components. The cabinet 120 is made of thermal insulation material and is used to freeze cells. A plurality of partitions 121 are fixed in an array on the inner wall of the cabinet 120, dividing the inner cavity of the cabinet 120 into multiple storage cavities 122, so that each cell dish can be stored independently, avoiding the situation where the cabinet 120 is opened when the cells are taken, causing a large amount of liquid nitrogen to be vaporized and affecting the storage environment of other cells.

[0026] Each storage cavity 122 corresponds to a sealed door 130, which includes a door panel 131 hingedly mounted on the cabinet body 120, a control panel 132 mounted on the door panel 131, and a handle 133 fixed on the door panel 131. The door panel 131 is used to control the opening and closing of the storage cavity 122, the control panel 132 is used to display the temperature data in the storage cavity 122 and control the first connecting pipe 160 and the second connecting pipe 190, and the handle 133 facilitates the user to open the door panel 131.

[0027] A temperature sensor 125 is mounted on the inner top wall of the storage cavity 122 for monitoring the ambient temperature within the storage cavity 122 and transmitting the data to the control panel 132 for display.

[0028] A liquid outlet channel 123 is provided at the bottom end of each storage cavity 122 and is connected to the first connecting pipe 160, so that the liquid nitrogen in the storage cavity 122 can enter the main liquid outlet pipe 150 through the first connecting pipe 160. The first connecting pipe 160 includes a first branch pipe 161 connecting the liquid outlet channel 123 and the main liquid outlet pipe 150 and an electric valve 162 installed on the first branch pipe 161. The first branch pipe 161 is used to introduce the liquid nitrogen from the liquid outlet channel 123 into the main liquid outlet pipe 150, and the electric valve 162 is used to control the opening and closing of the first branch pipe 161.

[0029] The box body 140 is made of thermal insulation material. Liquid nitrogen enters the box body 140 through the main liquid outlet pipe 150 and can be temporarily stored. The pump 170 is used to pump out the temporarily stored liquid nitrogen in the box body 140. The inlet of the pump 170 is connected to the inner cavity of the box body 140 through the water pumping pipe 171, and the outlet of the pump 170 is connected to the end of the main liquid inlet pipe 180, so as to facilitate the pumping of the liquid nitrogen in the box body 140 into the main liquid inlet pipe 180.

[0030] A liquid inlet channel 124 is provided at the top of each storage cavity 122 and is connected to the second connecting pipe 190. The second connecting pipe 190 includes a second branch pipe 191 connecting the liquid inlet channel 124 and the main liquid inlet pipe 180 and a flow valve 192 installed on the second branch pipe 191. The second branch pipe 191 is used to introduce the liquid nitrogen in the main liquid inlet pipe 180 into the liquid inlet channel 124. The flow valve 192 is used to control the opening and closing of the second branch pipe 191 and control the flow of liquid nitrogen passing through the second branch pipe 191 when the second branch pipe 191 is opened. By controlling the flow of liquid nitrogen entering the storage cavity 122, liquid chlorine is slowly injected to keep the temperature in the storage cavity 122 slowly decreasing, thereby preventing ice crystals from forming in the cells during the freezing process and causing damage to the cells.

[0031] The working principle and use process of the utility model are as follows: when taking cells, the first connecting pipe 160 corresponding to the cell storage cavity 122 is opened through the control panel 132. At this time, the electric valve 162 is opened, and the liquid nitrogen in the storage cavity 122 enters the box body 140 for temporary storage through the liquid outlet channel 123, the first connecting pipe 160, and the main liquid outlet pipe 150. The door panel 131 is opened to remove the cells in the storage cavity 122, and the door panel 131 is closed. At this time, the pump 170 is started, and the liquid nitrogen in the storage cavity 122 is temporarily stored. The water pipe 171 draws the liquid nitrogen out of the box 140 and pumps it back into the storage cavity 122 through the main liquid inlet pipe 180, the second connecting pipe 190 and the liquid inlet channel 124. At the same time, the temperature sensor 125 monitors the temperature in the storage cavity 122 in real time and controls the flow valve 192 to control the flow of liquid nitrogen entering the storage cavity 122. Liquid chlorine is slowly injected to keep the temperature in the storage cavity 122 slowly decreasing, thereby preventing ice crystals from forming during the freezing process and causing damage to the cells.

[0032] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and purpose of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A cell freezing drawer, comprising: The main body mechanism (100) is characterized in that the main body mechanism (100) comprises a base (110), a cabinet (120) fixed to the top of the base (110), a plurality of sealing doors (130) mounted on the cabinet (120), a box (140) mounted on the inner wall of the cabinet (120), a main liquid outlet pipe (150) arranged on one side of the cabinet (120) and connected to the box (140) at its bottom end, a plurality of first connecting pipes (160) mounted on one side of the main liquid outlet pipe (150), a pump (170) mounted on the inner wall of the base (110), a main liquid inlet pipe (180) arranged on the other side of the cabinet (120) and connected to an outlet of the pump (170), and a plurality of second connecting pipes (190) mounted on the main liquid inlet pipe (180); A plurality of partitions (121) are fixed in an array on the inner wall of the cabinet (120), dividing the inner cavity of the cabinet (120) into a plurality of storage cavities (122), each storage cavity (122) corresponding to a sealing door (130), a liquid outlet channel (123) is provided at the bottom end of the storage cavity (122) and communicates with the first connecting pipe (160), and a liquid inlet channel (124) is provided at the top end of the storage cavity (122) and communicates with the second connecting pipe (190); A temperature sensor (125) is installed on the inner top wall of the storage cavity (122), and the second connecting pipe (190) includes a second branch pipe (191) connecting the liquid inlet channel (124) and the main liquid inlet pipe (180), and a flow valve (192) installed on the second branch pipe (191).

2. A cell freezing drawer according to claim 1, characterized in that: The sealed door (130) comprises a door panel (131) hingedly mounted on the cabinet body (120), a control panel (132) mounted on the door panel (131), and a handle (133) fixed on the door panel (131).

3. A cell freezing drawer according to claim 1, characterized in that: The first connecting pipe (160) comprises a first branch pipe (161) connecting the liquid outlet channel (123) and the main liquid outlet pipe (150), and an electric valve (162) installed on the first branch pipe (161).

4. A cell freezing drawer according to claim 1, characterized in that: The inlet of the pump (170) is communicated with the inner cavity of the box (140) through a water pumping pipe (171), and the outlet of the pump (170) is communicated with the end of the main liquid inlet pipe (180).

5. The cell freezing drawer according to claim 1, characterized in that: The cabinet (120), the main liquid inlet pipe (180), the box (140) and the main liquid outlet pipe (150) are all made of thermal insulation materials.

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