Protein extraction separator capable of quantitatively filling

Abstract

The utility model discloses a protein extraction separator capable of quantitatively filling, which relates to the technical field of protein extraction separators and comprises a reaction tank and a connecting box, and a support frame is fixedly mounted on the outer side of the reaction tank. When the protein extraction separator capable of quantitatively filling works, an unquantitative reagent or solution is firstly put into a liquid storage cavity, the reagent or solution in the liquid storage cavity falls into a quantitative cylinder from an opening in the lower part of the liquid storage cavity, then a motor I is started to drive a gear to rotate, and the gear is in meshed connection with a moving rod; the gear enables the moving rod to pull the quantitative cylinder to move to the liquid guide groove, meanwhile, the two ends of the quantitative cylinder slide in the sliding grooves, then the baffle abuts against the opening of the liquid storage cavity, after the opening in the bottom end of the quantitative cylinder is aligned with the liquid guide groove, the reagent or the solution flows into the liquid guide groove from the quantitative cylinder, and finally the reagent or the solution flows into an inner cavity of the reaction tank through the material guide pipe. The reagent or the solution can be quantitatively injected through the connecting box and the quantitative cylinder, so that the working efficiency of the protein extraction separator is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein extraction and separation machines, in particular to a protein extraction and separation machine capable of quantitative filling. Background Art

[0002] Proteins are among the most important molecules in living organisms, playing a key role in nearly all life processes and biochemical reactions. The extraction, separation, and purification of proteins are crucial steps in biochemistry and molecular biology experiments, requiring the use of a protein extractor / separator. A protein extractor / separator is a device used to extract and separate proteins from biological samples. Its primary application areas include biomedical research, the food industry, and the pharmaceutical industry. This machine can help researchers and engineers purify specific proteins from complex biological mixtures, providing high-quality protein samples for subsequent analysis and applications.

[0003] However, existing protein extraction and separation machines have the following disadvantages. Since most protein extraction and separation machines need to add some reagents or solutions when releasing proteins from biological samples, these reagents or solutions are usually measured in a specified amount by the staff in advance and then placed in the protein extraction and separation machine. This is more troublesome and inconvenient to quickly refill the quantitative amount, which affects the working efficiency of the protein extraction and separation machine. Therefore, it is necessary to improve the existing protein extraction and separation machines. Utility Model Content

[0004] The purpose of the utility model is to provide a protein extraction and separation machine capable of quantitative filling, so as to solve the problem raised in the above background technology that the protein extraction and separation machines currently on the market are not convenient for rapid quantitative filling of reagents or solutions, which affects the working efficiency of the protein extraction and separation machines.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a protein extraction and separation machine that can be quantitatively filled, comprising a reaction tank and a connecting box, a support frame fixedly installed on the outside of the reaction tank, a feed pipe connected to the top of the reaction tank, and a discharge pipe connected to the bottom of the reaction tank, the connecting box is connected to the reaction tank through a bracket, a liquid storage cavity is provided inside the connecting box, a quantitative cylinder is provided at the lower opening of the liquid storage cavity, the quantitative cylinder is located in the built-in groove of the connecting box, one end of the quantitative cylinder is connected to a moving mechanism, the other end of the quantitative cylinder is connected to a baffle, and one end of the moving mechanism is located outside the connecting box.

[0006] Preferably, the moving mechanism includes a No. 1 motor, a gear and a moving rod, and one end of the quantitative cylinder is fixedly connected to the moving rod.

[0007] Preferably, one side of the moving rod is meshed with a gear, one side of the gear is connected to motor No. 1 through a coupling, and motor No. 1 is connected to the outside of the connection box.

[0008] Preferably, a slide groove is provided on the inner wall of the connection box near the quantitative cylinder, and the slide groove is slidably connected to the protrusions at both ends of the quantitative cylinder, and a liquid guide groove is provided inside the connection box near the bottom of the quantitative cylinder.

[0009] Preferably, a material guide pipe is connected below the liquid guide groove, one end of the material guide pipe is connected to the reaction tank, a stirring rod No. 1 is installed in the inner cavity of the reaction tank through a bearing, one end of the stirring rod No. 1 is connected to a filter plate through a bearing, and the filter plate is located in the inner cavity of the reaction tank.

[0010] Preferably, a No. 2 motor connected to the No. 1 stirring rod through a coupling is installed on the outside of the reaction tank, a No. 2 stirring rod is installed in the inner cavity of the reaction tank close to the No. 1 stirring rod through a bearing, and the end of the No. 2 stirring rod close to the outside of the reaction tank is connected to a transmission mechanism.

[0011] Preferably, the transmission mechanism includes a driven wheel, a belt, a No. 3 motor and a driving wheel, and one end of the No. 2 stirring rod is connected to the driven wheel and the driving wheel.

[0012] Preferably, belts are sleeved on the outer sides of the driven wheel and the driving wheel, and the end of the driving wheel away from the No. 2 stirring rod is connected to the No. 3 motor through a coupling.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. A moving mechanism and a quantitative cylinder are provided. First, an unquantified amount of reagent or solution is placed in the liquid storage chamber. The reagent or solution in the liquid storage chamber falls into the quantitative cylinder from the opening below the liquid storage chamber. Then the No. 1 motor is started to drive the gear to rotate. The gear is engaged with the moving rod. The gear causes the moving rod to pull the quantitative cylinder to move toward the liquid guide groove. At the same time, the two ends of the quantitative cylinder slide in the slide groove. Then the baffle is blocked at the opening of the liquid storage chamber to prevent the reagent or solution from flowing downward. After the bottom end opening of the quantitative cylinder is aligned with the liquid guide groove, a quantitative amount of reagent or solution flows from the quantitative cylinder into the liquid guide groove. Finally, the reagent or solution flows into the inner cavity of the reaction tank through the guide tube to mix and react with the biological sample. The connecting box and the quantitative cylinder can perform quantitative filling of the reagent or solution, thereby improving the working efficiency of the protein extraction and separation machine.

[0015] 2. A transmission mechanism and a No. 2 stirring rod are provided. While the No. 2 motor drives the No. 1 stirring rod to rotate, the No. 3 motor drives the driving wheel to rotate. The driving wheel then pulls the belt to drive the driven wheel to rotate. Then the driving wheel and the driven wheel drive the No. 2 stirring rod to rotate. The No. 2 stirring rod and the No. 1 stirring rod fully mix the biological sample and other components to prevent uneven reactions. The setting of multiple stirring rods improves the practicality of the protein extraction and separation machine. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the three-dimensional structure of the reaction tank of the utility model;

[0017] Figure 2 This is a schematic diagram of the three-dimensional cross-sectional structure of the reaction tank of the utility model;

[0018] Figure 3 This is a schematic diagram of the three-dimensional structure of the connection box of the utility model;

[0019] Figure 4 This is a schematic diagram of the three-dimensional cross-sectional structure of the connection box of the utility model;

[0020] Figure 5 This is a schematic diagram of the three-dimensional cross-sectional structure of the chute of the utility model;

[0021] Figure 6 This is a schematic diagram of the three-dimensional cross-sectional structure of the liquid guide groove of the utility model;

[0022] Figure 7 It is a schematic diagram of the three-dimensional structure of the transmission mechanism of the utility model.

[0023] In the figure: 1. Reaction tank; 2. Support frame; 3. Feed pipe; 4. Discharge pipe; 5. Connecting box; 6. Moving mechanism; 601. Motor No. 1; 602. Gear; 603. Moving rod; 7. Motor No. 2; 8. Transmission mechanism; 801. Driven pulley; 802. Belt; 803. Motor No. 3; 804. Driving pulley; 9. Feeding pipe; 10. Stirring rod No. 2; 11. Stirring rod No. 1; 12. Filter plate; 13. Liquid storage chamber; 14. Measuring cylinder; 15. Baffle; 16. Liquid guide trough; 17. Slide. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] See also Figures 1-6The utility model provides a technical solution: a protein extraction and separation machine that can be quantitatively filled, comprising a reaction tank 1 and a connecting box 5, a support frame 2 is fixedly installed on the outside of the reaction tank 1, a feed pipe 3 is connected to the top of the reaction tank 1, and a discharge pipe 4 is connected to the bottom of the reaction tank 1, the connecting box 5 is connected to the reaction tank 1 through a bracket, a No. 1 stirring rod 11 is installed in the inner cavity of the reaction tank 1 through a bearing, one end of the No. 1 stirring rod 11 is connected to a filter plate 12 through a bearing, and the filter plate 12 is located in the inner cavity of the reaction tank 1, a No. 2 motor 7 connected to the No. 1 stirring rod 11 through a coupling is installed on the outside of the reaction tank 1, and an ultrasonic oscillator is installed on the inner wall of the reaction tank 1.

[0026] See also Figures 1-6 , the interior of the connecting box 5 is provided with a liquid storage chamber 13, and a quantitative cylinder 14 is provided at the lower opening of the liquid storage chamber 13. The quantitative cylinder 14 is located in the built-in groove of the connecting box 5, and one end of the quantitative cylinder 14 is connected to the moving mechanism 6, and the other end of the quantitative cylinder 14 is connected to the baffle 15. One end of the moving mechanism 6 is located outside the connecting box 5, and the moving mechanism 6 includes a No. 1 motor 601, a gear 602 and a moving rod 603. One end of the quantitative cylinder 14 is fixedly connected to the moving rod 603, and one side of the moving rod 603 is meshed with a gear 602. One side of the gear 602 is connected to the moving rod 603. A No. 1 motor 601 is connected through a coupling, and the No. 1 motor 601 is connected to the outside of the connecting box 5. A slide groove 17 is provided on the inner wall of the connecting box 5 near the metering cylinder 14, and the slide groove 17 is slidably connected to the protrusions at both ends of the metering cylinder 14. A liquid guide groove 16 is provided inside the connecting box 5 near the bottom of the metering cylinder 14, and a material guide pipe 9 is connected to the bottom of the liquid guide groove 16. One end of the material guide pipe 9 is connected to the reaction tank 1. The movable rod 603 is provided with equally spaced tooth blocks on the side near the gear 602. The metering cylinder 14 is set to be cylindrical, and the cross-section of the liquid guide groove 16 is set to be inclined.

[0027] In specific implementation, since most protein extraction and separation machines need to add some reagents or solutions when releasing proteins from biological samples, these reagents or solutions are usually measured in a specified amount in advance by the staff and then put into the protein extraction and separation machine, which is more troublesome and inconvenient to quickly re-fill quantitatively, affecting the working efficiency of the protein extraction and separation machine. It can be done by first putting an unquantified amount of reagents or solutions into the liquid storage chamber 13, and the reagents or solutions in the liquid storage chamber 13 fall into the quantitative cylinder 14 from the opening below the liquid storage chamber 13, and then start the No. 1 motor 601 to drive the gear 602 to rotate, and the gear 602 and the moving rod 603 move forward. The gear 602 is meshed and connected, and the moving rod 603 pulls the quantitative cylinder 14 to move toward the liquid guide groove 16. At the same time, the two ends of the quantitative cylinder 14 slide in the slide groove 17. Then the baffle 15 abuts against the opening of the liquid storage chamber 13 to prevent the reagent or solution from flowing downward. After the bottom end opening of the quantitative cylinder 14 is aligned with the liquid guide groove 16, a quantitative amount of reagent or solution flows from the quantitative cylinder 14 into the liquid guide groove 16. Finally, the reagent or solution flows into the inner cavity of the reaction tank 1 through the guide tube 9 to mix and react with the biological sample. The connection box 5 and the quantitative cylinder 14 can perform quantitative filling of the reagent or solution, thereby improving the working efficiency of the protein extraction and separation machine.

[0028] See also Figure 1-Figure 3 and Figure 7 A No. 2 stirring rod 10 is installed in the inner cavity of the reaction tank 1 near the No. 1 stirring rod 11 through a bearing. The end of the No. 2 stirring rod 10 near the outside of the reaction tank 1 is connected to a transmission mechanism 8. The transmission mechanism 8 includes a driven wheel 801, a belt 802, a No. 3 motor 803 and a driving wheel 804. One end of the No. 2 stirring rod 10 is connected to the driven wheel 801 and the driving wheel 804. The outer sides of the driven wheel 801 and the driving wheel 804 are sleeved with a belt 802. The end of the driving wheel 804 away from the No. 2 stirring rod 10 is connected to the No. 3 motor 803 through a coupling.

[0029] During specific implementation, since a stirring rod is used in the protein extraction and separation machine to mix the biological sample with other components, but the stirring efficiency of one stirring rod is not high, the No. 3 motor 803 can drive the driving wheel 804 to rotate while the No. 2 motor 7 drives the No. 1 stirring rod 11 to rotate. The driving wheel 804 then pulls the belt 802 to drive the driven wheel 801 to rotate. Then the driving wheel 804 and the driven wheel 801 drive the No. 2 stirring rod 10 to rotate. The No. 2 stirring rod 10 and the No. 1 stirring rod 11 fully mix the biological sample and other components to prevent uneven reactions. The setting of multiple stirring rods improves the practicality of the protein extraction and separation machine.

[0030] Working principle: When using the protein extraction and separation machine with quantitative filling, first put the biological sample into the reaction tank 1 from the feed pipe 3, and then connect the box 5, the moving mechanism 6 and the quantitative cylinder 14 to quantitatively fill the reagent or solution into the reaction tank 1, and the No. 2 motor 7 drives the No. 1 stirring rod 11 to rotate, and at the same time the transmission mechanism 8 drives the No. 2 stirring rod 10 to rotate. The No. 1 stirring rod 11 and the No. 2 stirring rod 10 fully mix the biological sample with the reagent or solvent. During mixing, the ultrasonic oscillator breaks the structure of the biological sample. After the mixing is completed, the biological sample is precipitated, and the precipitated waste residue is blocked on the filter plate 12. Finally, the biological sample after precipitation and filtration is discharged from the discharge pipe 4, which is convenient for the subsequent completion of protein extraction and purification, thereby improving the working efficiency and practicality of the protein extraction and separation machine. The content not described in detail in the description belongs to the existing technology well known to professional and technical personnel in this field.

[0031] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A protein extraction and separation machine capable of quantitative filling, comprising a reaction tank (1) and a connection box (5), characterized in that: A support frame (2) is fixedly installed on the outside of the reaction tank (1), a feed pipe (3) is connected to the top of the reaction tank (1), and a discharge pipe (4) is connected to the bottom of the reaction tank (1). The connecting box (5) is connected to the reaction tank (1) through a bracket, and a liquid storage cavity (13) is provided inside the connecting box (5). A quantitative cylinder (14) is provided at the lower opening of the liquid storage cavity (13). The quantitative cylinder (14) is located in a built-in groove of the connecting box (5), one end of the quantitative cylinder (14) is connected to a moving mechanism (6), and the other end of the quantitative cylinder (14) is connected to a baffle (15). One end of the moving mechanism (6) is located outside the connecting box (5).

2. A protein extraction and separation machine capable of quantitative filling according to claim 1, characterized in that: The moving mechanism (6) comprises a No. 1 motor (601), a gear (602) and a moving rod (603), and one end of the metering cylinder (14) is fixedly connected to the moving rod (603).

3. A protein extraction and separation machine capable of quantitative filling according to claim 2, characterized in that: One side of the moving rod (603) is meshedly connected to a gear (602), one side of the gear (602) is connected to a No. 1 motor (601) via a coupling, and the No. 1 motor (601) is connected to the outside of the connection box (5).

4. The protein extraction and separation machine capable of quantitative filling according to claim 1, characterized in that: A chute (17) is provided on the inner wall of the connection box (5) near the dosing cylinder (14), and the chute (17) is slidably connected to the protrusions at both ends of the dosing cylinder (14). A liquid guide groove (16) is provided inside the connection box (5) near the bottom of the dosing cylinder (14).

5. The protein extraction and separation machine capable of quantitative filling according to claim 4, characterized in that: A material guide pipe (9) is connected below the liquid guide groove (16), one end of the material guide pipe (9) is connected to the reaction tank (1), a stirring rod (11) is installed in the inner cavity of the reaction tank (1) via a bearing, one end of the stirring rod (11) is connected to a filter plate (12) via a bearing, and the filter plate (12) is located in the inner cavity of the reaction tank (1).

6. The protein extraction and separation machine capable of quantitative filling according to claim 5, characterized in that: A second motor (7) connected to a first stirring rod (11) via a coupling is installed on the outside of the reaction tank (1); a second stirring rod (10) is installed in the inner cavity of the reaction tank (1) near the first stirring rod (11) via a bearing; and a transmission mechanism (8) is connected to one end of the second stirring rod (10) near the outside of the reaction tank (1).

7. The protein extraction and separation machine capable of quantitative filling according to claim 6, characterized in that: The transmission mechanism (8) comprises a driven wheel (801), a belt (802), a third motor (803) and a driving wheel (804), and one end of the second stirring rod (10) is connected to the driven wheel (801) and the driving wheel (804).

8. The protein extraction and separation machine capable of quantitative filling according to claim 7, characterized in that: The outer sides of the driven wheel (801) and the driving wheel (804) are sleeved with a belt (802), and the end of the driving wheel (804) away from the second stirring rod (10) is connected to the third motor (803) via a coupling.

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