Heating equipment for polypeptide synthesis

Abstract

The utility model relates to heating equipment for polypeptide synthesis, which comprises a heating box body, an upper frame body piece, a first heating piece, a liquid pumping piece, a second heating piece and a stirring piece, and the upper frame body piece is fixedly arranged above the heating box body; the first heating piece is fixedly arranged below the upper frame body piece; the number of the liquid pumping pieces is at least one, and the liquid pumping pieces are all arranged in the heating box body. The second heating piece is arranged at the bottom of the heating box body; the stirring piece is arranged on the heating box body. The technical scheme has the beneficial technical effects that the upper frame body part is arranged on the heating box body, the first heating part is arranged below the upper frame body part, the liquid pumping part is used for pumping polypeptide liquid to the upper frame body part from the bottom of the heating box body, and the first heating part is used for heating the polypeptide liquid discharged by the upper frame body part; meanwhile, the polypeptide liquid at the bottom of the heating box body is heated through the second heating part, and the polypeptide liquid at the bottom of the heating box body is stirred through the stirring part, so that the polypeptide liquid is heated more uniformly. And the problem of non-uniform heating is solved.

Description

Technical Field

[0001] The utility model relates to the field of polypeptide synthesis, in particular to heating equipment for polypeptide synthesis. Background Art

[0002] Peptide synthesis is a complex but important chemical synthesis method that involves linking multiple amino acids in a specific sequence to form a polypeptide chain. Peptides are compounds composed of multiple amino acids linked by peptide bonds, with a molecular structure intermediate between amino acids and proteins. The core principle of peptide synthesis is the condensation reaction between amino acids, where the α-carboxyl group of one amino acid reacts with the α-amino group of another via dehydration condensation to form a peptide bond. During the synthesis process, it is generally important to maintain a uniform temperature to avoid unwanted reactions.

[0003] Existing technologies, such as CN208485830U, provide a semi-automatic peptide synthesizer that uses a microwave heater and a temperature probe to heat a solution. A motor, rotating shaft, and rotating base are configured to thoroughly stir and mix the solution, ensuring complete peptide reaction and improving synthesis efficiency. However, this method suffers from uneven heating, with temperatures higher at the microwave heating location and lower at other locations. This leads to uneven heating and, in turn, low peptide synthesis efficiency.

[0004] Therefore, it is very necessary to provide a heating device for polypeptide synthesis to solve the above technical problems. Utility Model Content

[0005] Based on the above description, the present invention provides a heating device for polypeptide synthesis to solve the problem of uneven heating in the prior art, that is, the temperature is higher at the microwave heating position and lower at other positions, which leads to uneven heating and thus low polypeptide synthesis efficiency.

[0006] The technical solution of the utility model for solving the above-mentioned technical problems is as follows: A heating device for polypeptide synthesis includes a heating box, an upper frame, a first heating element, a liquid pumping element, a second heating element and a stirring element. The upper frame is fixedly arranged above the heating box, for accommodating polypeptide liquid and playing a role in fixing the frame; the first heating element is fixedly arranged below the upper frame, for heating the polypeptide liquid discharged from the upper frame; there is at least one liquid pumping element, and all of the liquid pumping elements are arranged in the heating box, for transporting the polypeptide liquid at the bottom of the heating box to the upper frame; the second heating element is arranged at the bottom of the heating box, for heating the polypeptide liquid at the bottom of the heating box; the stirring element is arranged at the bottom of the heating box, for stirring the polypeptide liquid at the bottom of the heating box.

[0007] Furthermore, the upper frame body includes a storage block, a liquid collecting tank, a liquid outlet and an external connecting rod, the storage block is arranged above the heating box body; there is at least one liquid collecting tank, and the liquid collecting tank is arranged in the middle of the storage block; the liquid outlet is arranged in the liquid collecting tank; there are several external connecting rods, one end of each of the external connecting rods is fixedly connected to the heating box body, and the other end is fixedly connected to the storage block.

[0008] Furthermore, the first heating element includes a connecting bolt, a heating coil, a thermometer receiving groove and a first temperature sensor, the connecting bolt is fixed on the liquid outlet; the heating coil is sleeved on the connecting bolt; the thermometer receiving groove is provided on the heating coil, and the thermometer receiving groove is provided with at least one; the first temperature sensor is fixed in the thermometer receiving groove and is used to detect the temperature at the thermometer receiving groove.

[0009] Furthermore, the stirring element includes a stirring motor and a stirring blade. The stirring motor is provided with a fixed end and a rotating end. The fixed end of the stirring motor is fixed on the storage block; the stirring blade is fixed on the rotating end of the stirring motor.

[0010] Furthermore, a pump body receiving groove is provided on the heating box body, and the pump body receiving groove is arranged in a one-to-one correspondence with the pump liquid component.

[0011] Furthermore, the liquid pump component includes a pump body, a liquid inlet pipe and a liquid outlet pipe, and the pump body is fixed in the pump body receiving tank; one end of the liquid inlet pipe is connected to the water inlet of the pump body, and the other end of the liquid inlet pipe is arranged at the bottom of the heating box body, for inputting the polypeptide liquid at the bottom of the heating box body into the pump body; one end of the liquid outlet pipe is connected to the water outlet of the pump body, and the other end of the liquid outlet pipe is connected to the liquid collecting tank.

[0012] Furthermore, it also includes a heat-insulating sleeve, which is arranged in the pump body receiving groove and is sleeved on the liquid inlet pipe and the liquid outlet pipe to keep the polypeptide liquid in the liquid inlet pipe and the liquid outlet pipe warm.

[0013] Furthermore, a heating chamber is provided at the bottom of the heating box.

[0014] Furthermore, the second heating element includes a heating ring and a second temperature sensor, and the heating ring is provided with at least one, and the heating ring is fixed in the heating chamber; the second temperature sensor is arranged corresponding to the heating ring, and the second temperature sensor is fixed on the heating chamber for detecting the temperature at the heating ring.

[0015] Furthermore, it also includes a cover, transparent glass and a box port, the cover is arranged on the heating box; the transparent glass is fixed on the cover, and the transparent glass is coated with a heat insulation coating; the box port is arranged on one side of the heating box.

[0016] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0017] By arranging an upper frame on the heating box, and a first heating element below the upper frame, a liquid pump is used to pump the polypeptide liquid from the bottom of the heating box to the upper frame, and the polypeptide liquid discharged from the upper frame is heated by the first heating element; at the same time, a second heating element is used to heat the polypeptide liquid at the bottom of the heating box, and a stirring element is used to stir the polypeptide liquid at the bottom of the heating box, so that the polypeptide liquid is heated more evenly. This effectively solves the problem of uneven heating in the prior art, that is, the temperature is higher at the microwave heating position and lower at other positions, which leads to uneven heating and thus low polypeptide synthesis efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is one of the overall structural diagrams of a heating device for polypeptide synthesis provided in an embodiment of the present utility model;

[0019] Figure 2 The second schematic diagram of the overall structure of a heating device for polypeptide synthesis provided by an embodiment of the present utility model;

[0020] Figure 3 A schematic diagram of the structure of a heating device for polypeptide synthesis without the cover provided in an embodiment of the present invention;

[0021] Figure 4 for Figure 3 Enlarged schematic diagram of the middle Q;

[0022] Figure 5 A schematic diagram of the structure of a heating device for polypeptide synthesis provided in an embodiment of the present invention without the bottom;

[0023] Figure 6 for Figure 5 Schematic diagram of the cross section at W in the middle;

[0024] Figure 7 A partial schematic diagram of a heating chamber of a heating device for polypeptide synthesis provided in an embodiment of the present utility model.

[0025] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0026] 1. Heating box; 11. Pump housing tank; 12. Heating chamber; 13. Cover; 14. Transparent glass; 15. Box port;

[0027] 2. Upper frame; 21. Storage block; 22. Liquid collecting tank; 23. Liquid outlet; 24. External connecting rod;

[0028] 3. First heating element; 31. Connecting bolt; 32. Heating coil; 33. Thermometer receiving groove; 34. First temperature sensor;

[0029] 4. Pump parts; 41. Pump body; 42. Liquid inlet pipe; 43. Liquid outlet pipe; 44. Insulation sleeve;

[0030] 5. Second heating element; 51. Heating ring; 52. Second temperature sensor;

[0031] 6. Stirring element; 61. Stirring motor; 62. Stirring blade. DETAILED DESCRIPTION

[0032] To facilitate understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. The accompanying drawings provide embodiments of the present application. However, the present application may be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of the present application more thorough and comprehensive.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application.

[0034] It will be understood that spatial relational terms such as "under," "beneath," "below," "under," "above," "above," etc., may be used herein to describe the relationship of an element or feature shown in the figures to other elements or features. It will be understood that in addition to the orientations shown in the figures, spatial relational terms also include different orientations of the device in use and operation. For example, if the device in the drawings is turned over, the element or feature described as "under" or "beneath" or "beneath" the other elements will be oriented as "above" the other elements or features. Thus, the exemplary terms "under" and "under" may include both upper and lower orientations. In addition, the device may also include alternative orientations (e.g., rotated 90 degrees or other orientations), and the spatial descriptors used herein are interpreted accordingly.

[0035] It should be noted that when an element is considered to be "connected" to another element, it can be directly connected to the other element or connected to the other element through an intermediate element. In the following embodiments, "connection" should be understood as "electrical connection", "communication connection", etc., if the connected circuits, modules, units, etc. can transmit electrical signals or data to each other.

[0036] When used herein, the singular forms "a", "an", and "the" may also include the plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "include / comprise" or "have" and the like specify the presence of stated features, integers, steps, operations, components, parts, or combinations thereof, but do not preclude the possibility of the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

[0037] like Figures 1 to 7 As shown, a heating device for polypeptide synthesis includes a heating box 1, an upper frame body 2, a first heating element 3, a liquid pumping element 4, a second heating element 5 and a stirring element 6. The upper frame body 2 is fixedly arranged above the heating box 1, for accommodating the polypeptide liquid and playing a role in fixing the frame; the first heating element 3 is fixedly arranged below the upper frame body 2, for heating the polypeptide liquid discharged from the upper frame body 2; there is at least one liquid pumping element 4, and the liquid pumping elements 4 are all arranged in the heating box 1, for transporting the polypeptide liquid at the bottom of the heating box 1 to the upper frame body 2; the second heating element 5 is arranged at the bottom of the heating box 1, for heating the polypeptide liquid at the bottom of the heating box 1; the stirring element 6 is arranged at the bottom of the heating box 1, for stirring the polypeptide liquid at the bottom of the heating box 1.

[0038] In this embodiment, an upper frame 2 is provided on the heating box 1, a first heating element 3 is provided below the upper frame 2, a liquid pump 4 is used to pump the polypeptide liquid from the bottom of the heating box 1 to the upper frame 2, and the first heating element 3 is used to heat the polypeptide liquid discharged from the upper frame 2; at the same time, a second heating element 5 is used to heat the polypeptide liquid at the bottom of the heating box 1, and a stirring element 6 is used to stir the polypeptide liquid at the bottom of the heating box 1, so that the polypeptide liquid is heated more evenly. This effectively solves the problem of uneven heating in the prior art, that is, the temperature is higher at the microwave heating position and lower at other positions, which leads to uneven heating and thus low polypeptide synthesis efficiency.

[0039] In some embodiments, the upper shelf body 2 includes a storage block 21, a liquid collecting tank 22, a liquid outlet 23 and an external connecting rod 24, the storage block 21 is arranged above the heating box body 1; there is at least one liquid collecting tank 22, and the liquid collecting tank 22 is arranged in the middle of the storage block 21; the liquid outlet 23 is arranged in the liquid collecting tank 22; there are several external connecting rods 24, one end of the several external connecting rods 24 is fixedly connected to the heating box body 1, and the other end is fixedly connected to the storage block 21.

[0040] In this embodiment, a plurality of external connecting rods 24 are provided to firmly secure the receiving block 21 to the heating box 1. Furthermore, protrusions are provided on all sides and in the center of the receiving block 21. Thus, while the liquid collecting trough 22 is provided, a position for securing the stirring member 6 is also provided.

[0041] In some embodiments, the first heating element 3 includes a connecting bolt 31, a heating coil 32, a thermometer receiving groove 33 and a first temperature sensor 34, wherein the connecting bolt 31 is fixed on the liquid outlet 23; the heating coil 32 is sleeved on the connecting bolt 31; the thermometer receiving groove 33 is provided on the heating coil 32, and at least one thermometer receiving groove 33 is provided; the first temperature sensor 34 is fixed in the thermometer receiving groove 33 for detecting the temperature at the thermometer receiving groove 33.

[0042] In this embodiment, a connecting bolt 31 is fixed to the liquid outlet 23. A through hole is provided in the middle of the heating coil 32, through which the connecting bolt 31 is inserted. Thus, the connecting bolt 31 secures the heating coil 32 to the liquid outlet 23. Since the liquid outlet 23 is located within the liquid collecting tank 22, the polypeptide liquid within the collecting tank 22 flows out and lands on the heating coil 32. The heating coil 32 then heats the polypeptide liquid. Simultaneously, a first temperature sensor 34 measures the temperature of the heating coil 32 and the polypeptide liquid. The first temperature sensor 34 is a WZP-PT100 temperature sensor.

[0043] In some embodiments, the stirring member 6 includes a stirring motor 61 and a stirring blade 62 . The stirring motor 61 is provided with a fixed end and a rotating end. The fixed end of the stirring motor 61 is fixed on the storage block 21 ; the stirring blade 62 is fixed on the rotating end of the stirring motor 61 .

[0044] In this embodiment, the polypeptide liquid is stirred by the cooperation of the stirring motor 61 and the stirring blade 62, so that the heating temperature of the polypeptide liquid is more uniform. At the same time, the stirring motor 61 is model HG-KR43J.

[0045] In some embodiments, a pump body receiving groove 11 is provided on the heating box 1 , and the pump body receiving groove 11 is arranged in a one-to-one correspondence with the pump liquid component 4 .

[0046] In some embodiments, the liquid pump component 4 includes a pump body 41, a liquid inlet pipe 42 and a liquid outlet pipe 43. The pump body 41 is fixed in the pump body receiving tank 11; one end of the liquid inlet pipe 42 is connected to the water inlet of the pump body 41, and the other end of the liquid inlet pipe 42 is arranged at the bottom of the heating box 1, for inputting the polypeptide liquid at the bottom of the heating box 1 into the pump body; one end of the liquid outlet pipe 43 is connected to the water outlet of the pump body 41, and the other end of the liquid outlet pipe 43 is connected to the liquid collecting tank 22.

[0047] In this embodiment, the pump body 41, the liquid inlet pipe 42 and the liquid outlet pipe 43 are all arranged in the pump body receiving tank 11, and the pump body 41 pumps the polypeptide liquid at the bottom of the heating box 1 into the liquid collecting tank 22. In addition, the pump body 41 is an MP10RN micro pump.

[0048] In some embodiments, a heat-insulating sleeve 44 is further included. The heat-insulating sleeve 44 is disposed in the pump body receiving tank 11 and is sleeved on the liquid inlet pipe 42 and the liquid outlet pipe 43 to keep the polypeptide liquid in the liquid inlet pipe 42 and the liquid outlet pipe 43 warm.

[0049] In this embodiment, in order to reduce the temperature loss of the polypeptide liquid in the liquid inlet pipe 42 and the liquid outlet pipe 43, a thermal insulation sleeve 44 is provided to keep the polypeptide liquid in the liquid inlet pipe 42 and the liquid outlet pipe 43 warm.

[0050] In some embodiments, a heating chamber 12 is provided at the bottom of the heating box 1 .

[0051] In some embodiments, the second heating element 5 includes a heating ring 51 and a second temperature sensor 52. The heating ring 51 is provided with at least one, and the heating ring 51 is fixed in the heating chamber 12; the second temperature sensor 52 is arranged corresponding to the heating ring 51, and the second temperature sensor 52 is fixed on the heating chamber 12 for detecting the temperature at the heating ring 51.

[0052] In this embodiment, a heating ring 51 is provided in the heating chamber 12 to heat the polypeptide liquid at the bottom of the heating chamber 1. A second temperature sensor 52 is used to measure the temperature of the heating ring 51 and the polypeptide liquid at the bottom of the heating chamber 1. Furthermore, if a local overtemperature is detected, the controller controls the heating ring 51 to reduce the heating power in the corresponding area, which also falls within the scope of protection of this application.

[0053] In some embodiments, it also includes a cover 13, a transparent glass 14 and a box port 15, wherein the cover 13 is arranged on the heating box 1; the transparent glass 14 is fixed on the cover 13, and the transparent glass 14 is coated with a heat-insulating coating; the box port 15 is arranged on one side of the heating box 1.

[0054] In this embodiment, transparent glass 14 is provided on lid 13, allowing the operator to observe the interior of heating chamber 1 through transparent glass 14. Furthermore, transparent glass 14 is coated with a heat-insulating coating to reduce temperature loss within heating chamber 1. Furthermore, a chamber port 15 is provided to drain the polypeptide liquid within heating chamber 1.

[0055] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0056] By arranging an upper frame on the heating box, and a first heating element below the upper frame, a liquid pump is used to pump the polypeptide liquid from the bottom of the heating box to the upper frame, and the polypeptide liquid discharged from the upper frame is heated by the first heating element; at the same time, a second heating element is used to heat the polypeptide liquid at the bottom of the heating box, and a stirring element is used to stir the polypeptide liquid at the bottom of the heating box, so that the polypeptide liquid is heated more evenly. This effectively solves the problem of uneven heating in the prior art, that is, the temperature is higher at the microwave heating position and lower at other positions, which leads to uneven heating and thus low polypeptide synthesis efficiency.

[0057] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A heating device for polypeptide synthesis, characterized in that: include: Heating box (1); An upper frame member (2) is fixedly arranged above the heating box (1) and is used to contain the polypeptide liquid and to fix the frame; a first heating element (3) fixedly disposed below the upper frame body (2) and used for heating the polypeptide liquid discharged from the upper frame body (2); a liquid pumping member (4), of which at least one is provided, and each of the liquid pumping members (4) is arranged in the heating box (1), and is used to transport the polypeptide liquid at the bottom of the heating box (1) to the upper frame member (2); a second heating element (5), which is provided at the bottom of the heating box (1) and is used to heat the polypeptide liquid at the bottom of the heating box (1); A stirring member (6) is provided at the bottom of the heating box (1) and is used to stir the polypeptide liquid at the bottom of the heating box (1).

2. A heating device for polypeptide synthesis according to claim 1, characterized in that: The upper frame member (2) comprises: A storage block (21) is provided above the heating box (1); a liquid collecting trough (22), at least one of which is provided, and the liquid collecting trough (22) is provided in the middle of the receiving block (21); a liquid outlet (23) disposed in the liquid collecting tank (22); A plurality of external connecting rods (24) are provided, one end of each of the external connecting rods (24) is fixedly connected to the heating box (1), and the other end is fixedly connected to the receiving block (21).

3. A heating device for polypeptide synthesis according to claim 2, characterized in that: The first heating element (3) comprises: a connecting bolt (31) fixed on the liquid outlet hole (23); A heating ring (32) is sleeved on the connecting bolt (31); A thermometer receiving groove (33) is provided on the heating ring (32), and the thermometer receiving groove (33) is provided with at least one; A first temperature sensor (34) is fixedly disposed in the thermometer receiving groove (33) and is used to detect the temperature at the thermometer receiving groove (33).

4. A heating device for polypeptide synthesis according to claim 2, characterized in that: The stirring member (6) comprises: A stirring motor (61) is provided with a fixed end and a rotating end, wherein the fixed end of the stirring motor (61) is fixed on the receiving block (21); A stirring blade (62) is fixed to the rotating end of the stirring motor (61).

5. A heating device for polypeptide synthesis according to claim 2, characterized in that: The heating box (1) is provided with a pump body receiving groove (11), and the pump body receiving groove (11) is arranged in a one-to-one correspondence with the pump liquid component (4).

6. A heating device for polypeptide synthesis according to claim 5, characterized in that: The pumping element (4) comprises: A pump body (41) fixed in the pump body receiving groove (11); a liquid inlet pipe (42), one end of which is connected to the water inlet of the pump body (41), and the other end of which is arranged at the bottom of the heating box (1), for inputting the polypeptide liquid at the bottom of the heating box (1) into the pump body; A liquid outlet pipe (43) has one end connected to the water outlet of the pump body (41) and the other end connected to the liquid collecting tank (22).

7. A heating device for polypeptide synthesis according to claim 6, characterized in that: Also includes: The heat-insulating sleeve (44) is arranged in the pump body receiving groove (11) and is sleeved on the liquid inlet pipe (42) and the liquid outlet pipe (43) to keep the polypeptide liquid in the liquid inlet pipe (42) and the liquid outlet pipe (43) warm.

8. The heating device for polypeptide synthesis according to claim 1, characterized in that: A heating chamber (12) is provided at the bottom of the heating box (1).

9. A heating device for polypeptide synthesis according to claim 8, characterized in that: The second heating element (5) comprises: a heating ring (51), at least one of which is provided, wherein the heating ring (51) is fixed in the heating chamber (12); A second temperature sensor (52) is provided corresponding to the heating ring (51), and the second temperature sensor (52) is fixed on the heating chamber (12) and is used to detect the temperature at the heating ring (51).

10. The heating device for polypeptide synthesis according to claim 1, characterized in that: Also includes: a cover (13) disposed on the heating box (1); Transparent glass (14) is fixed on the cover (13), and the transparent glass (14) is coated with a heat-insulating coating; The box port (15) is provided on one side of the heating box (1).

Images