Novel culture mold for 3d cell culture for toxicological research

Abstract

The utility model discloses a novel culture mould for 3d cell culture capable of being used for toxicological research, which belongs to the technical field of cell culture moulds and comprises a micropore mould, a partition mould is arranged on the micropore mould, a plurality of culture chambers are arranged inside the partition mould, a plurality of micro holes are arranged on the culture chambers, and the micro holes are communicated with the micropore mould. According to the three-dimensional cell culture mould, a plurality of cells with different drug concentrations can be cultured in the same mould at the same time, so that the operation amount and complexity of an experiment are greatly reduced, the preparation operation process of a three-dimensional cell structure is simplified, and the production efficiency is improved. According to the invention, the experimental error is reduced, the drug concentration can be ensured not to interfere with each other between partitions, the experimental efficiency is improved, a plurality of samples can be treated in the same mold at the same time, the experimental cost is reduced, and the consumption of reagents and materials is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture molds, in particular to a novel culture mold for 3D cell culture which can be used for toxicology research. Background Art

[0002] Cell biology experimental molds are widely used in a variety of processes, particularly in basic biological research, providing a crucial aid for conducting experiments. Compared to traditional two-dimensional cell culture, three-dimensional cell culture better simulates the in vivo environment, improving experimental reliability and accuracy. It more closely resembles true physiological conditions, facilitating the study of cell-cell interactions, cell morphology and function, and the effects of drugs on cells. Within the mold, cells can grow, reproduce, and interact in three dimensions, forming structures and functions similar to in vivo tissues.

[0003] The preparation of three-dimensional cell structures requires the use of microporous molds. While existing microporous molds can efficiently generate cells, they only allow the use of a single drug or concentration during drug treatment, making multi-drug or multi-concentration experiments complex. To simplify the process of treating 3D cells with different drug concentrations, a mold with multiple compartments can be used to ensure that drug concentrations do not interfere with each other. A new 3D cell culture mold for toxicology studies has been proposed. Utility Model Content

[0004] The utility model aims to solve at least one of the technical problems existing in the prior art.

[0005] To this end, one purpose of the present invention is to propose a new type of 3D cell culture mold that can be used for toxicology research. This new type of 3D cell culture mold that can be used for toxicology research can ensure that drug concentrations do not interfere with each other between compartments, thereby improving experimental efficiency, and can process multiple samples simultaneously in the same mold, thereby reducing experimental costs and reducing the consumption of reagents and materials.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a new type of culture mold for 3D cell culture that can be used for toxicology research, including a microporous mold, a preparation block is provided on the microporous mold, a plurality of partition areas are opened inside the preparation block, and a plurality of culture chambers are integrally formed at the bottom of the preparation block.

[0007] Preferably, the diameter of each culture chamber is 400 μm.

[0008] Preferably, the number of the preparation blocks is 4 or 8.

[0009] Preferably, the material of the preparation block is silica gel.

[0010] Compared with the prior art, the beneficial effects of the present invention are:

[0011] The utility model can simultaneously culture multiple cells with different drug concentrations in the same mold, thereby greatly reducing the amount and complexity of experimental operations, simplifying the preparation operation process of three-dimensional cell structures, reducing experimental errors, ensuring that drug concentrations do not interfere with each other between compartments, improving experimental efficiency, and being able to process multiple samples simultaneously in the same mold, reducing experimental costs and reducing the consumption of reagents and materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a schematic structural diagram of a novel 3D cell culture mold that can be used for toxicology research in the present utility model;

[0013] Figure 2 This is a schematic structural diagram of a culture chamber in a new culture mold for 3D cell culture that can be used for toxicology research in the present utility model;

[0014] Figure 3 This utility model is a new type of 3D cell culture mold that can be used for toxicology research Figure 2 Schematic diagram of the enlarged structure of area A in .

[0015] In the figure: 1. Microporous mold; 2. Preparation block; 3. Separation area; 4. Culture chamber. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0017] See also Figure 1-Figure 3 An embodiment of the present invention provides a novel 3D cell culture mold that can be used for toxicology research, including a microporous mold 1, a preparation block 2 is provided on the microporous mold 1, a plurality of partition areas 3 are opened inside the preparation block 2, and a plurality of culture chambers 4 are integrally formed at the bottom of the preparation block 2.

[0018] When in use, DMEM / F-12 culture medium is added to the multiple culture chambers 4 for immersion. The multiple culture chambers 4 can be used to form and prepare cell clusters required for various three-dimensional cell cultures. The multiple culture chambers 4 are separated by the partition area 3 so that different drug concentrations will not interfere with each other.

[0019] The number of culture chambers 3 can be set to 4 or 8 according to experimental requirements.

[0020] According to the above technical solution, the working steps of this solution are summarized and sorted out: When the utility model is in use, DMEM / F-12 culture medium is added to multiple culture chambers 4 for immersion and standby use. Multiple culture chambers 4 can be used for the formation and preparation of cell clusters required for various three-dimensional cell cultures. The multiple culture chambers 4 are separated by the partition area 3 so that different drug concentrations will not interfere with each other.

[0021] Any portion not described in the present invention is the same as the prior art or can be implemented using the prior art. Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A novel 3D cell culture mold for toxicology research, comprising a microporous mold (1), characterized in that: A preparation block (2) is provided on the microporous mold (1), a plurality of partitions (3) are provided inside the preparation block (2), and a plurality of culture chambers (4) are integrally formed at the bottom of the preparation block (2).

2. A novel 3D cell culture mold for toxicology research according to claim 1, characterized in that: The diameter of each of the culture chambers (4) is 400 μm.

3. The novel 3D cell culture mold for toxicology research according to claim 1, characterized in that: The number of the preparation blocks (2) is 4 or 8.

4. The novel 3D cell culture mold for toxicology research according to claim 1, characterized in that: The material of the preparation block (2) is silica gel.

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